Spg026594 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg026594
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Calcium-binding EF-hand family protein
Location	scaffold6: 18450779 .. 18454137 (-)
RNA-Seq Expression	Spg026594
Synteny	Spg026594

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGCGAAGAACCAGAGACCCCAATCGGAATCCGGATTCGAGGACTGGCTGCCCGTGATGGCCTGCAACCTCGGCGGAGAGGGGCTGATCGGAGAGCTCTGCAACGGATTCAATCTGCTGATGGACAGAGAGACAGGAGTCATCAATTTCGACAGCTTGAAGAGGAATGCGGCCGCGCTAGGCCTCGAGGATCTCACCGACGACGAGCTCAGAGGGATGTTGAGGGAAGGGGATTCCGACGGCGATGGGGCTCTCAATCAGATGGACTTTTGTGTTCTTATGTTCAGATTAAGCCCCGATTTGATGGACGCCTCTCGATACTGGTTCCAACAAACTCTCCGCCCAGGGAATTTCTCTTGATTCTTTTTTTTTTTTTTTTTTAATAAATGGTTATTAAATAATTAAAATTTGGAACTAATTCATGCACTTTCAATACTCAGATTTACTCCCCTGCTTCAGTTTAGTTGGAAGAGTCTATGTCTCCTTTGGTGGATCAGATTTACCTTCCCACAATTTTGTTCCATACTCTTAACAAACTTACATAACTTTTTAGTAATAAATTTAAATGGAGTTCACGATTCCATTCTAGTTTAATGGATAAGATATCTATTGTTGTTCTAAAGCGTCATTAGTTGCATCTCCACCCTACAATCATCCAACTAAAAATTTAATTGAAATAAGAATGATTTTGTAAGAGTGTAAAATATCATTTTGGAGAGAGATGTGTATGAAATTTAATTTGTGTTAATACAGATAGTGTCCACTTTTTTTCTTTTTTCTTTTTTTTTTTTTGAGTTCAAACTAATAAATACATTCGACTTGCAAGTGGGTTTGATTTTGAATTAAAAATAATATTGAGTTTATTACAACAATATCTACAAATAATTTCTTGTGCAATTACCCTATTTCTTATCATAAGTTTCTTATTTTTTTAAACAACAAAACCAAAGGAATAAATTGCCACACACGTTTACCATAACTTTACTCAAAGGCGCTCAATAATTCAACCTGCTTGTAGATTTACCTCTCTACTATCATGAAAAACTAAATTTGAAAACGAGTCATCCAATGAAACTATTTCATATTTTCAAATATGTGTTTGGTAGCAGATTCATAAATTAGATCATAATTTAAACAATTGTTCAAGATGTGTTTGATCATATATTTGTACTTATCAACTAAATACTAAGTTGAATATAAACTATTATTAATTAATTTTTAAAAATGATATGTGGTATAAATTTTTTTATAATTATTATTTTACAATATCATCCAATTTATAATACAAATTTAATTTTACAAAAAACAATTGAGTTTATAACCTGAAATAATATATTTATTAATTTTTTTTTTTATCTTTTTTAATTTAATTATTCATTTAAAATTTTCAAATTCAAATTCTGAATACATTGAAAACATAAACCTTTATTATATGTATCTGTTTTATACTTGACGCTCATCCATCTATTATACGTATCTGTTTTATTTTTTCTTTTGGATGAGTAAACGAGAAATAATTAACTCTTATGAAGCTATGCTTCTTCCATTTTATCTTTTTTTTTTTTTTTTTGTTGAGTTCATCATTTTAAGATGAGAGATTCAAACCTCTGATTTCTTGATCGATGGTACATGCTAATTATTGTTGAGTTATGCTTACTTTGACCCTTCTATTTTATAAACATGAAATTTCAAAATCGTTAAGAATATTTTATTTTAATTAGGTTGAAAATTTATGAAACTGATTTCAATAATTATCGGGTTCACACCCAACTGAGGTATAAGACCAATTCTTTTTGTAATAGCCATAGCTTTACCATTTGATAGTTGGAATTTTTTAAATATAAAGACAGTTGATAGTTGGAAACAATTTAAAGCTAATAATAGTCAAAGTCACTCCCATTTACCATTTGTGATTGCGATCAGTTAACTTTTAGGGAAAATACACTTTTAGTCCCTGAGATTTGAGTTAGGTTTCATTTTGATCCCTGAGTTTTCAAACTCAACAATTTAGTTCCTGAGGTTTCATTTTGGTTTCATTTTGGTCCATCCATCCAATTTTCCGTTAAAAACTAATGGACCGATGACGTGACCCTATTTTTTTTATTTAAAAAATTATTTTTAAAAATTTTTAATTAAAAATATATTAAAGTAATAAAAATATTATTATTTTTATTTCTTTTATTACTTCTCTCTCTACCATCTTCTTCTTCCCCTTCATCTTCTTCAAACCAAGGATGAAAATATCGATATCAGTACTGATATTGGTAACTCAGATTTACGGATATATCGACGGATATTCTGGTAAAAATGACGGATATCATGGAAACTCATAAAATTATTCTGATTTTTTTTATGAAACTTTGAACTAAGCTTATTTAAGTAATAAACTATTAGTTTAAGCATACATATCAATAAGGGCAAAATGCACTTTTGGTCCCCGAAGTTTGGAGTTGATGCCTATTTGGTCCCTGAAGTTTCAAAACATACACTTTAGTCCTCCAGGTTTGTAAAATCATGCTAAATGGTCCCTGACTTAACTTTAACTTAACGGAATGTTGACTGGACCGTTATGTGCTGATGTGGATATGATGACAAGGCCCTCATTTGCTTATTAGGCACATCAATTAAAAAAATAAAATAAAAAACTAAACTCTTAAAACAAAGAGGAGATGGGTTCTGGTTAAAAAAAAGTAAAAAAAGAAAGTCACATCAATTAACCCATCCCCAATCTTTGTTCCTCATCATTTGAGATTCAGGTGCACTGTTCATGGAGATGGGTTCGTGAAGATTGGGGAAGAATTGGGTTTCTGTTGTCTTCTCTTTGTCTACACTGTATTAGCAATTAATAATGAGAAGTTTTGGATCTTCGTTTTACCAAAACAGCTCTGCGATTTTTCTGTTGACTTCATGTCCACGGCGGCCTTAGTGGTGGACCAGTACATGCGAGCTCCACCGTCCGTCGGTCACGTGATGCTGCTCCGGCAGATTCTCTCCGGCGGAGTTCGATTGCGGATGGTCGATTTGTCGGGGGAATTTCAATAAGGAGAAATAGTTGCGCAAGTTTTTCAATCAGGCAAACTAGCCACTCAAGTTTTTCTGTTCATTGCCTCCAGGATTCACATGAAGATTCGGAGCGTCCCGATGAGGTAGGGCGAAAATCGGCCGGGCAAATACTCAGTACGAGACGAGCCGTGCTCGGTGTTCCATTAATTGCAATTGGCGCACAGTTTCTACAATCAGCAGTGGTCAGAGCGGAAGAGAAGTCGCCGGAGACCGTGACTCCGGTGCTGGAGGCGGTGACATCGCCGTTGCCGTTGCCGTCGACTACGAGCCGTGCTTGGTGTTCCATGGGTTAA

mRNA sequence

ATGGCCGCGAAGAACCAGAGACCCCAATCGGAATCCGGATTCGAGGACTGGCTGCCCGTGATGGCCTGCAACCTCGGCGGAGAGGGGCTGATCGGAGAGCTCTGCAACGGATTCAATCTGCTGATGGACAGAGAGACAGGAGTCATCAATTTCGACAGCTTGAAGAGGAATGCGGCCGCGCTAGGCCTCGAGGATCTCACCGACGACGAGCTCAGAGGGATGTTGAGGGAAGGGGATTCCGACGGCGATGGGGCTCTCAATCAGATGGACTTTTGTGTTCTTATGTTCAGATTAAGCCCCGATTTGATGGACGCCTCTCGATACTGGTGCACTGTTCATGGAGATGGGTTCGTGAAGATTGGGGAAGAATTGGGTTTCTGTTGTCTTCTCTTTGTCTACACTGTATTAGCAATTAATAATGAGAAGTTTTGGATCTTCGTTTTACCAAAACAGCTCTGCGATTTTTCTGTTGACTTCATGTCCACGGCGGCCTTAGTGGTGGACCAGTACATGCGAGCTCCACCGTCCGTCGGTCACGTGATGCTGCTCCGGCAGATTCTCTCCGGCGGAGTTCGATTGCGGATGGATTCACATGAAGATTCGGAGCGTCCCGATGAGGTAGGGCGAAAATCGGCCGGGCAAATACTCAGTACGAGACGAGCCGTGCTCGGTGTTCCATTAATTGCAATTGGCGCACAGTTTCTACAATCAGCAGTGGTCAGAGCGGAAGAGAAGTCGCCGGAGACCGTGACTCCGGTGCTGGAGGCGGTGACATCGCCGTTGCCGTTGCCGTCGACTACGAGCCGTGCTTGGTGTTCCATGGGTTAA

Coding sequence (CDS)

ATGGCCGCGAAGAACCAGAGACCCCAATCGGAATCCGGATTCGAGGACTGGCTGCCCGTGATGGCCTGCAACCTCGGCGGAGAGGGGCTGATCGGAGAGCTCTGCAACGGATTCAATCTGCTGATGGACAGAGAGACAGGAGTCATCAATTTCGACAGCTTGAAGAGGAATGCGGCCGCGCTAGGCCTCGAGGATCTCACCGACGACGAGCTCAGAGGGATGTTGAGGGAAGGGGATTCCGACGGCGATGGGGCTCTCAATCAGATGGACTTTTGTGTTCTTATGTTCAGATTAAGCCCCGATTTGATGGACGCCTCTCGATACTGGTGCACTGTTCATGGAGATGGGTTCGTGAAGATTGGGGAAGAATTGGGTTTCTGTTGTCTTCTCTTTGTCTACACTGTATTAGCAATTAATAATGAGAAGTTTTGGATCTTCGTTTTACCAAAACAGCTCTGCGATTTTTCTGTTGACTTCATGTCCACGGCGGCCTTAGTGGTGGACCAGTACATGCGAGCTCCACCGTCCGTCGGTCACGTGATGCTGCTCCGGCAGATTCTCTCCGGCGGAGTTCGATTGCGGATGGATTCACATGAAGATTCGGAGCGTCCCGATGAGGTAGGGCGAAAATCGGCCGGGCAAATACTCAGTACGAGACGAGCCGTGCTCGGTGTTCCATTAATTGCAATTGGCGCACAGTTTCTACAATCAGCAGTGGTCAGAGCGGAAGAGAAGTCGCCGGAGACCGTGACTCCGGTGCTGGAGGCGGTGACATCGCCGTTGCCGTTGCCGTCGACTACGAGCCGTGCTTGGTGTTCCATGGGTTAA

Protein sequence

MAAKNQRPQSESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAALGLEDLTDDELRGMLREGDSDGDGALNQMDFCVLMFRLSPDLMDASRYWCTVHGDGFVKIGEELGFCCLLFVYTVLAINNEKFWIFVLPKQLCDFSVDFMSTAALVVDQYMRAPPSVGHVMLLRQILSGGVRLRMDSHEDSERPDEVGRKSAGQILSTRRAVLGVPLIAIGAQFLQSAVVRAEEKSPETVTPVLEAVTSPLPLPSTTSRAWCSMG

Homology

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BLAST of Spg026594 vs. NCBI nr
Match: XP_022144078.1 (calcium-binding protein PBP1-like [Momordica charantia])

HSP 1 Score: 202.2 bits (513), Expect = 5.7e-48
Identity = 96/109 (88.07%), Postives = 101/109 (92.66%), Query Frame = 0


Query: 1   MAAKNQRPQSESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAA 60
           MAAKNQ PQS+SGFEDWLP MA  LGGEGLIGELCNGFNLLMDRE GVINF+SLKRNAAA
Sbjct: 1   MAAKNQSPQSQSGFEDWLPAMAWKLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLEDLTDDELRGMLREGDSDGDGALNQMDFCVLMFRLSPDLMDASRYW 110
           LGL DL+D+ELRGML EGD DGDGALNQM+FCVLMFRLSPDLMDASRYW
Sbjct: 61  LGLGDLSDEELRGMLTEGDFDGDGALNQMEFCVLMFRLSPDLMDASRYW 109

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BLAST of Spg026594 vs. NCBI nr
Match: XP_004152909.1 (calcium-binding protein KRP1 [Cucumis sativus] >KGN56165.1 hypothetical protein Csa_010073 [Cucumis sativus])

HSP 1 Score: 198.0 bits (502), Expect = 1.1e-46
Identity = 95/108 (87.96%), Postives = 101/108 (93.52%), Query Frame = 0


Query: 1   MAAKNQRPQSESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAA 60
           M  KNQ PQSESGF+DWLPVMA NLGGEGLIGELCNGFNLLMDRE GVINF+SLKRNAAA
Sbjct: 1   MTPKNQIPQSESGFQDWLPVMAGNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLEDLTDDELRGMLREGDSDGDGALNQMDFCVLMFRLSPDLMDASRY 109
           LGL DL+DDELRGMLREGD DGDGALNQM+FCVLMFRLSP+LM+ASRY
Sbjct: 61  LGLGDLSDDELRGMLREGDFDGDGALNQMEFCVLMFRLSPELMEASRY 108

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BLAST of Spg026594 vs. NCBI nr
Match: XP_038905287.1 (calcium-binding protein KRP1-like [Benincasa hispida])

HSP 1 Score: 193.4 bits (490), Expect = 2.7e-45
Identity = 93/108 (86.11%), Postives = 99/108 (91.67%), Query Frame = 0


Query: 1   MAAKNQRPQSESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAA 60
           MA+KNQ PQSES FEDWLP MA NLGGEGLIGELCNGFNLLMDRE GVINF+SLKRNAA 
Sbjct: 1   MASKNQIPQSESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAV 60

Query: 61  LGLEDLTDDELRGMLREGDSDGDGALNQMDFCVLMFRLSPDLMDASRY 109
           LGL DLTD+ELRGMLREGD DGDGALN+M+FCVLMFRLSP LM+ASRY
Sbjct: 61  LGLGDLTDEELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRY 108

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BLAST of Spg026594 vs. NCBI nr
Match: XP_022932158.1 (calcium-binding protein PBP1-like [Cucurbita moschata])

HSP 1 Score: 191.8 bits (486), Expect = 7.7e-45
Identity = 92/108 (85.19%), Postives = 97/108 (89.81%), Query Frame = 0


Query: 1   MAAKNQRPQSESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAA 60
           MA  NQ PQSESGFEDWLP MAC LGGEGLIGELCNGFNLLMD E GVIN +SLKRNAAA
Sbjct: 65  MAGNNQIPQSESGFEDWLPEMACKLGGEGLIGELCNGFNLLMDPEKGVINLESLKRNAAA 124

Query: 61  LGLEDLTDDELRGMLREGDSDGDGALNQMDFCVLMFRLSPDLMDASRY 109
           LGL D +D+ELRGMLREGD DGDGALNQM+FCVLMFRLSP+LMDASRY
Sbjct: 125 LGLGDQSDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPELMDASRY 172

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BLAST of Spg026594 vs. NCBI nr
Match: KAG6577444.1 (Calcium-binding protein KRP1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015516.1 Calcium-binding protein PBP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 191.8 bits (486), Expect = 7.7e-45
Identity = 92/108 (85.19%), Postives = 97/108 (89.81%), Query Frame = 0


Query: 1   MAAKNQRPQSESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAA 60
           MA  NQ PQSESGFEDWLP MAC LGGEGLIGELCNGFNLLMD E GVIN +SLKRNAAA
Sbjct: 1   MAGNNQIPQSESGFEDWLPEMACKLGGEGLIGELCNGFNLLMDPEKGVINLESLKRNAAA 60

Query: 61  LGLEDLTDDELRGMLREGDSDGDGALNQMDFCVLMFRLSPDLMDASRY 109
           LGL D +D+ELRGMLREGD DGDGALNQM+FCVLMFRLSP+LMDASRY
Sbjct: 61  LGLGDQSDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPELMDASRY 108

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BLAST of Spg026594 vs. ExPASy Swiss-Prot
Match: O81831 (Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 4.0e-36
Identity = 83/122 (68.03%), Postives = 93/122 (76.23%), Query Frame = 0


Query: 6   QRPQSESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAA-LGLE 65
           Q PQ    F D+LP MA NLGGEGLIGELCNGF LLMDRE GVI F+SL+RNAAA LGL 
Sbjct: 13  QNPQ--PNFHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLG 72

Query: 66  DLTDDELRGMLREGDSDGDGALNQMDFCVLMFRLSPDLMDASRYWCTVHGDGFVKIGEEL 125
           DLTD+++R M++EGD D DGALNQM+FCVLMFRLSPDLM+ASR   T        I EE 
Sbjct: 73  DLTDEDVRCMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEASRCLVT------EVIEEEF 126

Query: 126 GF 127
           GF
Sbjct: 133 GF 126

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BLAST of Spg026594 vs. ExPASy Swiss-Prot
Match: Q9LSQ6 (Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 6.0e-32
Identity = 69/105 (65.71%), Postives = 84/105 (80.00%), Query Frame = 0


Query: 4   KNQRPQSESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAA-ALG 63
           +NQ PQ    F+D+ P MA  LGGEGLI E+C GF LLMD++ GVI F+SL+RNA+  LG
Sbjct: 13  ENQEPQ----FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLG 72

Query: 64  LEDLTDDELRGMLREGDSDGDGALNQMDFCVLMFRLSPDLMDASR 108
           L DLTDD++R M+ EGD D DGALNQM+FCVLMFRLSP+LM+ASR
Sbjct: 73  LGDLTDDDVRYMINEGDFDRDGALNQMEFCVLMFRLSPELMEASR 113

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BLAST of Spg026594 vs. ExPASy Swiss-Prot
Match: Q9ZPX9 (Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2)

HSP 1 Score: 112.5 bits (280), Expect = 7.8e-24
Identity = 51/99 (51.52%), Postives = 72/99 (72.73%), Query Frame = 0


Query: 11  ESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAALGLEDLTDDE 70
           E+ +ED LPVMA  +  E  + ELC GF+LL D E  +I  +SL+RN+  LG+E ++ ++
Sbjct: 19  ETKYEDMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEGMSKED 78

Query: 71  LRGMLREGDSDGDGALNQMDFCVLMFRLSPDLMDASRYW 110
            +GM+REGD DGDGALNQ +FCVLM RLSP++M+ +  W
Sbjct: 79  AQGMVREGDLDGDGALNQTEFCVLMVRLSPEMMEDAETW 117

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BLAST of Spg026594 vs. ExPASy Swiss-Prot
Match: Q06827 (Caltractin OS=Scherffelia dubia OX=3190 PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.9e-10
Identity = 36/86 (41.86%), Postives = 53/86 (61.63%), Query Frame = 0


Query: 14  FEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAALGLEDLTDDELRG 73
           FE++L +M   +G      E+   F L  D ETG I+F +LKR A  LG E++TD+EL+ 
Sbjct: 82  FEEFLQMMTAKMGERDSREEIMKAFRLFDDDETGKISFKNLKRVAKELG-ENMTDEELQE 141

Query: 74  MLREGDSDGDGALNQMDFCVLMFRLS 100
           M+ E D DGDG +N+ +F  +M + S
Sbjct: 142 MIDEADRDGDGEVNEEEFFRIMKKTS 166

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BLAST of Spg026594 vs. ExPASy Swiss-Prot
Match: P43646 (Caltractin (Fragment) OS=Tetraselmis striata OX=3165 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.9e-10
Identity = 36/86 (41.86%), Postives = 53/86 (61.63%), Query Frame = 0


Query: 14  FEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAALGLEDLTDDELRG 73
           FE++L +M   +G      E+   F L  D ETG I+F +LKR A  LG E++TD+EL+ 
Sbjct: 62  FEEFLQMMTAKMGERDSREEIMKAFRLFDDDETGKISFKNLKRVAKELG-ENMTDEELQE 121

Query: 74  MLREGDSDGDGALNQMDFCVLMFRLS 100
           M+ E D DGDG +N+ +F  +M + S
Sbjct: 122 MIDEADRDGDGEVNEEEFFRIMKKTS 146

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BLAST of Spg026594 vs. ExPASy TrEMBL
Match: A0A6J1CSD5 (calcium-binding protein PBP1-like OS=Momordica charantia OX=3673 GN=LOC111013856 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 2.8e-48
Identity = 96/109 (88.07%), Postives = 101/109 (92.66%), Query Frame = 0


Query: 1   MAAKNQRPQSESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAA 60
           MAAKNQ PQS+SGFEDWLP MA  LGGEGLIGELCNGFNLLMDRE GVINF+SLKRNAAA
Sbjct: 1   MAAKNQSPQSQSGFEDWLPAMAWKLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLEDLTDDELRGMLREGDSDGDGALNQMDFCVLMFRLSPDLMDASRYW 110
           LGL DL+D+ELRGML EGD DGDGALNQM+FCVLMFRLSPDLMDASRYW
Sbjct: 61  LGLGDLSDEELRGMLTEGDFDGDGALNQMEFCVLMFRLSPDLMDASRYW 109

[Close]

BLAST of Spg026594 vs. ExPASy TrEMBL
Match: A0A0A0L2H0 (EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G081900 PE=4 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 5.2e-47
Identity = 95/108 (87.96%), Postives = 101/108 (93.52%), Query Frame = 0


Query: 1   MAAKNQRPQSESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAA 60
           M  KNQ PQSESGF+DWLPVMA NLGGEGLIGELCNGFNLLMDRE GVINF+SLKRNAAA
Sbjct: 1   MTPKNQIPQSESGFQDWLPVMAGNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLEDLTDDELRGMLREGDSDGDGALNQMDFCVLMFRLSPDLMDASRY 109
           LGL DL+DDELRGMLREGD DGDGALNQM+FCVLMFRLSP+LM+ASRY
Sbjct: 61  LGLGDLSDDELRGMLREGDFDGDGALNQMEFCVLMFRLSPELMEASRY 108

[Close]

BLAST of Spg026594 vs. ExPASy TrEMBL
Match: A0A6J1F0V4 (calcium-binding protein PBP1-like OS=Cucurbita moschata OX=3662 GN=LOC111438477 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 3.7e-45
Identity = 92/108 (85.19%), Postives = 97/108 (89.81%), Query Frame = 0


Query: 1   MAAKNQRPQSESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAA 60
           MA  NQ PQSESGFEDWLP MAC LGGEGLIGELCNGFNLLMD E GVIN +SLKRNAAA
Sbjct: 65  MAGNNQIPQSESGFEDWLPEMACKLGGEGLIGELCNGFNLLMDPEKGVINLESLKRNAAA 124

Query: 61  LGLEDLTDDELRGMLREGDSDGDGALNQMDFCVLMFRLSPDLMDASRY 109
           LGL D +D+ELRGMLREGD DGDGALNQM+FCVLMFRLSP+LMDASRY
Sbjct: 125 LGLGDQSDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPELMDASRY 172

[Close]

BLAST of Spg026594 vs. ExPASy TrEMBL
Match: A0A6J1JCW0 (calcium-binding protein PBP1-like OS=Cucurbita maxima OX=3661 GN=LOC111483323 PE=4 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 4.9e-45
Identity = 92/108 (85.19%), Postives = 97/108 (89.81%), Query Frame = 0


Query: 1   MAAKNQRPQSESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAA 60
           MA  NQ PQSESGFEDWLP MAC LGGEGLIGELCNGFNLLMD E GVIN +SLKRNAAA
Sbjct: 1   MAGNNQIPQSESGFEDWLPEMACKLGGEGLIGELCNGFNLLMDPEKGVINLESLKRNAAA 60

Query: 61  LGLEDLTDDELRGMLREGDSDGDGALNQMDFCVLMFRLSPDLMDASRY 109
           LGL D +D+ELRGMLREGD DGDGALNQM+FCVLMFRLSP+LMDASRY
Sbjct: 61  LGLGDHSDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPELMDASRY 108

[Close]

BLAST of Spg026594 vs. ExPASy TrEMBL
Match: A0A5A7SII1 (Calcium-binding protein PBP1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G00340 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 8.4e-45
Identity = 91/108 (84.26%), Postives = 99/108 (91.67%), Query Frame = 0


Query: 1   MAAKNQRPQSESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAA 60
           M + N  PQSESGF+DWLPVMA  LGGEGLIGELCNGFNLLMDRE GVINF+SLKRNAAA
Sbjct: 1   MTSNNHIPQSESGFQDWLPVMAGKLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLEDLTDDELRGMLREGDSDGDGALNQMDFCVLMFRLSPDLMDASRY 109
           LGL DL+DDELRGMLREGD +GDGALNQM+FCVLMFRLSP+LM+ASRY
Sbjct: 61  LGLGDLSDDELRGMLREGDLNGDGALNQMEFCVLMFRLSPELMEASRY 108

[Close]

BLAST of Spg026594 vs. TAIR 10
Match: AT4G27280.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 153.3 bits (386), Expect = 2.8e-37
Identity = 83/122 (68.03%), Postives = 93/122 (76.23%), Query Frame = 0


Query: 6   QRPQSESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAA-LGLE 65
           Q PQ    F D+LP MA NLGGEGLIGELCNGF LLMDRE GVI F+SL+RNAAA LGL 
Sbjct: 13  QNPQ--PNFHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLG 72

Query: 66  DLTDDELRGMLREGDSDGDGALNQMDFCVLMFRLSPDLMDASRYWCTVHGDGFVKIGEEL 125
           DLTD+++R M++EGD D DGALNQM+FCVLMFRLSPDLM+ASR   T        I EE 
Sbjct: 73  DLTDEDVRCMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEASRCLVT------EVIEEEF 126

Query: 126 GF 127
           GF
Sbjct: 133 GF 126

[Close]

BLAST of Spg026594 vs. TAIR 10
Match: AT5G54490.1 (pinoid-binding protein 1 )

HSP 1 Score: 139.4 bits (350), Expect = 4.2e-33
Identity = 69/105 (65.71%), Postives = 84/105 (80.00%), Query Frame = 0


Query: 4   KNQRPQSESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAA-ALG 63
           +NQ PQ    F+D+ P MA  LGGEGLI E+C GF LLMD++ GVI F+SL+RNA+  LG
Sbjct: 13  ENQEPQ----FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLG 72

Query: 64  LEDLTDDELRGMLREGDSDGDGALNQMDFCVLMFRLSPDLMDASR 108
           L DLTDD++R M+ EGD D DGALNQM+FCVLMFRLSP+LM+ASR
Sbjct: 73  LGDLTDDDVRYMINEGDFDRDGALNQMEFCVLMFRLSPELMEASR 113

[Close]

BLAST of Spg026594 vs. TAIR 10
Match: AT2G46600.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 112.5 bits (280), Expect = 5.6e-25
Identity = 51/99 (51.52%), Postives = 72/99 (72.73%), Query Frame = 0


Query: 11  ESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAALGLEDLTDDE 70
           E+ +ED LPVMA  +  E  + ELC GF+LL D E  +I  +SL+RN+  LG+E ++ ++
Sbjct: 19  ETKYEDMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEGMSKED 78

Query: 71  LRGMLREGDSDGDGALNQMDFCVLMFRLSPDLMDASRYW 110
            +GM+REGD DGDGALNQ +FCVLM RLSP++M+ +  W
Sbjct: 79  AQGMVREGDLDGDGALNQTEFCVLMVRLSPEMMEDAETW 117

[Close]

BLAST of Spg026594 vs. TAIR 10
Match: AT3G50360.1 (centrin2 )

HSP 1 Score: 50.8 bits (120), Expect = 2.0e-06
Identity = 29/84 (34.52%), Postives = 46/84 (54.76%), Query Frame = 0


Query: 14  FEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAALGLEDLTDDELRG 73
           F++++ +M   +G      EL   F ++   + G I+ D +KR A  LG E+ TD E+R 
Sbjct: 81  FDEFVHMMTAKIGERDTKEELTKAFQIIDLDKNGKISPDDIKRMAKDLG-ENFTDAEIRE 140

Query: 74  MLREGDSDGDGALNQMDFCVLMFR 98
           M+ E D D DG +N  +F  +M R
Sbjct: 141 MVEEADRDRDGEVNMDEFMRMMRR 163

[Close]

BLAST of Spg026594 vs. TAIR 10
Match: AT4G37010.1 (centrin 2 )

HSP 1 Score: 48.9 bits (115), Expect = 7.6e-06
Identity = 29/99 (29.29%), Postives = 48/99 (48.48%), Query Frame = 0


Query: 1   MAAKNQRPQSESGFEDWLPVMACNLGGEGLIGELCNGFNLLMDRETGVINFDSLKRNAAA 60
           MA  ++       F++++ +M    G    I EL   F ++     G I+   +K  A  
Sbjct: 68  MAEVDKNQSGAIDFDEFVHMMTTKFGERDSIDELSKAFKIIDHDNNGKISPRDIKMIAKE 127

Query: 61  LGLEDLTDDELRGMLREGDSDGDGALNQMDFCVLMFRLS 100
           LG E+ TD+++  M+ E D D DG +N  +F  +M R S
Sbjct: 128 LG-ENFTDNDIEEMIEEADRDKDGEVNLEEFMKMMKRTS 165

The following BLAST results are available for this feature:

BLAST of Spg026594 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144078.1	5.7e-48	88.07	calcium-binding protein PBP1-like [Momordica charantia]	[more]
XP_004152909.1	1.1e-46	87.96	calcium-binding protein KRP1 [Cucumis sativus] >KGN56165.1 hypothetical protein ...	[more]
XP_038905287.1	2.7e-45	86.11	calcium-binding protein KRP1-like [Benincasa hispida]	[more]
XP_022932158.1	7.7e-45	85.19	calcium-binding protein PBP1-like [Cucurbita moschata]	[more]
KAG6577444.1	7.7e-45	85.19	Calcium-binding protein KRP1, partial [Cucurbita argyrosperma subsp. sororia] >K...	[more]

BLAST of Spg026594 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81831	4.0e-36	68.03	Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1	[more]
Q9LSQ6	6.0e-32	65.71	Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1	[more]
Q9ZPX9	7.8e-24	51.52	Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2	[more]
Q06827	2.9e-10	41.86	Caltractin OS=Scherffelia dubia OX=3190 PE=1 SV=1	[more]
P43646	2.9e-10	41.86	Caltractin (Fragment) OS=Tetraselmis striata OX=3165 PE=2 SV=1	[more]

BLAST of Spg026594 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CSD5	2.8e-48	88.07	calcium-binding protein PBP1-like OS=Momordica charantia OX=3673 GN=LOC111013856...	[more]
A0A0A0L2H0	5.2e-47	87.96	EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G081900 PE=...	[more]
A0A6J1F0V4	3.7e-45	85.19	calcium-binding protein PBP1-like OS=Cucurbita moschata OX=3662 GN=LOC111438477 ...	[more]
A0A6J1JCW0	4.9e-45	85.19	calcium-binding protein PBP1-like OS=Cucurbita maxima OX=3661 GN=LOC111483323 PE...	[more]
A0A5A7SII1	8.4e-45	84.26	Calcium-binding protein PBP1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]

BLAST of Spg026594 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27280.1	2.8e-37	68.03	Calcium-binding EF-hand family protein	[more]
AT5G54490.1	4.2e-33	65.71	pinoid-binding protein 1	[more]
AT2G46600.1	5.6e-25	51.52	Calcium-binding EF-hand family protein	[more]
AT3G50360.1	2.0e-06	34.52	centrin2	[more]
AT4G37010.1	7.6e-06	29.29	centrin 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 45..97 e-value: 1.7E-16 score: 59.7
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 66..101 score: 9.464338
None	No IPR available	GENE3D	1.10.238.10		coord: 11..115 e-value: 4.7E-18 score: 67.2
None	No IPR available	PANTHER	PTHR47319:SF6	CALCIUM-BINDING PROTEIN KRP1	coord: 9..110
IPR044205	Calcium-binding protein KIC/PBP1/KRP1	PANTHER	PTHR47319	CALCIUM-BINDING PROTEIN KIC	coord: 9..110
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 79..91
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 13..95

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg026594.1	Spg026594.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding