Spg026251 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg026251
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRNase H domain-containing protein
Locationscaffold13: 14862359 .. 14864011 (+)
RNA-Seq ExpressionSpg026251
SyntenySpg026251
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGGATCAAAAGTAATGAGGATTCTTCTTCTTTGGATAGAAATCGACGGTGGTCTCCTCCGAATGGCGTATTTTGGAAGCTTAACTCTGATGCTGCATGTTCGAAAGAGGTCTCGATGACAAGTCTTGGGATGATCTGCAGAAATTTTGAGGGTAAAGTTATGGTAGTTGCGTCAAAGGCTTTGGACGTGAGGTTGGAGGCCCATATTGCTCAATTATGGGCCATCCTTGAAGGTTTAAAGCTGGTTATTTCATTGGGCTGCACTATGTTAGTTGTGGAGTCCGATTGTCAACTTGCTATCAATTTCATCAATAAGGTTTCTCTTATTTGGTCAGATTCAGAGGCCATAGTTCTGTCTATTTGGAGCCTCTTACCTTTTTTTTAGGGATATCTCTTTCAATTTTGCTCCTAGATCTTGTAATAGGGTTGCCCATGAGATTGCTCGATATGGGAAATATGTAAATAGCCATGACGTTTGGGTCTCTAATTTCTCAAACTGGATGTGTATGTGGGTCGAGTATGACCGTTCATTTTTAGCCCATGTGGCGTTATAATATGAGGTATTATTTTTCAAAAAAAAAAAAAAAAAAAGCTTAAGGTTCCTTAAAGAGTGAAAGTTTTCCTCTAGACTTTTGCTTACAAGAATTCAAATTCTCGTTGATCACTTTTTTTTTTTTTTTTTATAGAAAACCAGCTTTCATGGAGAAAACATGAAAGAATACAAGGGCATACAAAAAGACCAAGCCCATAGAAGGGAGTCCTCTTAAAGGAAGGGGCTCCAATCCAATAAGAAAAGACCTAAAGGATAATTACAAAATAAATTTGTAACCGAGGCCCAAAAGGAAGCATGGAATCTGGATAAAGACCATACATCGCCCGGGCTTCTAACTATCCCCCTAAACACTCTGTTATTTCTCTCCCCCCATAAGTTCCATAAGAGCGCACACATGTCGGCCTACCATAAAAATCTTCCTTTGCCATGGAGGGTCAGATTGAGGAGGAACTCTTGACACTCTCAGATCTAGGTAGTTGATCCGTTGCTGCCCAAACGCATCAAAGAAAAGGTCCCAACGGGAGCGAACAAAGTCACAGCTCCAAAGAATATGATCCAAATCTTCCTCCACCTTCTGACAAAGGATGGAACAAAAGGGGCCAAACAAATGGGCATTCCTTCTCAAGAGCCTACCAAAGGTATTAACCCTACCATGGAGAACTTTCCAGACAAAAATCCTTACCTTCTTCGACACTTTCACCTTCCACATGAAAGAAAAGATAGACTTGCTATAGGGAGGATGATTCAACAAAGAAGTGAAGTAGGATTTACATGAGAACCCGACAGAAGGATCAGGGCTTCAACAACGAAAATCTCTCCTCCCCAACCAAAAGATAGACTCGCATTTTTGGAATGCTCCTTGACTATATCTTCGAGCTCAAACTCTCGAAGATATAGTTGTTGATCACCCAAGTCTTTTCAGATTGTGGTATCAAGTTAGTTGGTCCAGACTTTTGCAAAATCTTTTGGAATGTCTACGTGCACAGTGATGTGGATATTGTCCTTCCTGAACTCTCATTAAGTGAGTGGATCAAGGGAAAGGCTAGGTTCACGTGGAAGAGTGCGGCTAGAACTATTCCTTTGGTCTCTTTGGATTGA

mRNA sequence

ATGCGGATCAAAAGTAATGAGGATTCTTCTTCTTTGGATAGAAATCGACGGTGGTCTCCTCCGAATGGCGTATTTTGGAAGCTTAACTCTGATGCTGCATGTTCGAAAGAGGTCTCGATGACAAGTCTTGGGATGATCTGCAGAAATTTTGAGGGTAAAGTTATGGTAGTTGCGTCAAAGGCTTTGGACGTGAGGTTGGAGGCCCATATTGCTCAATTATGGGCCATCCTTGAAGGTTTAAAGCTGGTTATTTCATTGGGCTGCACTATGTTAGTTGTGGAGTCCGATTGTCAACTTGCTATCAATTTCATCAATAAGGTAGTTGATCCGTTGCTGCCCAAACGCATCAAAGAAAAGGTCCCAACGGGAGCGAACAAAGTCACAGCTCCAAAGAATATGATCCAAATCTTCCTCCACCTTCTGACAAAGGATGGAACAAAAGGGGCCAAACAAATGGGCATTCCTTCTCAAGAGCCTACCAAAGATTGTGGTATCAAGTTAGTTGGTCCAGACTTTTGCAAAATCTTTTGGAATGTCTACGTGCACAGTGATGTGGATATTGTCCTTCCTGAACTCTCATTAAGTGAGTGGATCAAGGGAAAGGCTAGGTTCACGTGGAAGAGTGCGGCTAGAACTATTCCTTTGGTCTCTTTGGATTGA

Coding sequence (CDS)

ATGCGGATCAAAAGTAATGAGGATTCTTCTTCTTTGGATAGAAATCGACGGTGGTCTCCTCCGAATGGCGTATTTTGGAAGCTTAACTCTGATGCTGCATGTTCGAAAGAGGTCTCGATGACAAGTCTTGGGATGATCTGCAGAAATTTTGAGGGTAAAGTTATGGTAGTTGCGTCAAAGGCTTTGGACGTGAGGTTGGAGGCCCATATTGCTCAATTATGGGCCATCCTTGAAGGTTTAAAGCTGGTTATTTCATTGGGCTGCACTATGTTAGTTGTGGAGTCCGATTGTCAACTTGCTATCAATTTCATCAATAAGGTAGTTGATCCGTTGCTGCCCAAACGCATCAAAGAAAAGGTCCCAACGGGAGCGAACAAAGTCACAGCTCCAAAGAATATGATCCAAATCTTCCTCCACCTTCTGACAAAGGATGGAACAAAAGGGGCCAAACAAATGGGCATTCCTTCTCAAGAGCCTACCAAAGATTGTGGTATCAAGTTAGTTGGTCCAGACTTTTGCAAAATCTTTTGGAATGTCTACGTGCACAGTGATGTGGATATTGTCCTTCCTGAACTCTCATTAAGTGAGTGGATCAAGGGAAAGGCTAGGTTCACGTGGAAGAGTGCGGCTAGAACTATTCCTTTGGTCTCTTTGGATTGA

Protein sequence

MRIKSNEDSSSLDRNRRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVMVVASKALDVRLEAHIAQLWAILEGLKLVISLGCTMLVVESDCQLAINFINKVVDPLLPKRIKEKVPTGANKVTAPKNMIQIFLHLLTKDGTKGAKQMGIPSQEPTKDCGIKLVGPDFCKIFWNVYVHSDVDIVLPELSLSEWIKGKARFTWKSAARTIPLVSLD
Homology
BLAST of Spg026251 vs. NCBI nr
Match: XP_040996092.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC121242268 [Juglans microcarpa x Juglans regia])

HSP 1 Score: 76.6 bits (187), Expect = 2.9e-10
Identity = 40/93 (43.01%), Postives = 58/93 (62.37%), Query Frame = 0

Query: 17  RWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVMVVASKALDVRLEAHIAQLWAI 76
           RWSPP+    KLN D A   E++ TSLG I RN  G V++  S+A  V+LE    +  A+
Sbjct: 317 RWSPPSQDSLKLNIDGALFFELNKTSLGAILRNHVGDVLMAVSRAKSVQLELEQVEAAAL 376

Query: 77  LEGLKLVISLGCTMLVVESDCQLAINFINKVVD 110
           L GL+L +++G + LV+ESDC   +  +NKV +
Sbjct: 377 LRGLQLCLNIGISKLVIESDCLFLVEEVNKVAE 409

BLAST of Spg026251 vs. NCBI nr
Match: OMO72757.1 (hypothetical protein CCACVL1_17615 [Corchorus capsularis])

HSP 1 Score: 70.9 bits (172), Expect = 1.6e-08
Identity = 39/89 (43.82%), Postives = 53/89 (59.55%), Query Frame = 0

Query: 17   RWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVMVVASKALDVRLEAHIAQLWAI 76
            RWSPP    WK+N+DAA  K++ +  LG + R+FEG+V+    K +    +   A L+AI
Sbjct: 1029 RWSPPAVGSWKINTDAAFDKDLGVAGLGAVLRDFEGRVLCSIIKKMHFIQDPLFAVLYAI 1088

Query: 77   LEGLKLVISLGCTMLVVESDCQLAINFIN 106
              GL+L  S G    V+ESDC LAI  IN
Sbjct: 1089 QLGLQLARSEGFLTCVIESDCLLAITAIN 1117

BLAST of Spg026251 vs. NCBI nr
Match: XP_023888246.1 (uncharacterized protein LOC112000364 [Quercus suber])

HSP 1 Score: 68.6 bits (166), Expect = 7.9e-08
Identity = 36/102 (35.29%), Postives = 58/102 (56.86%), Query Frame = 0

Query: 12  LDRNRRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVMVVASKALDVRLEAHIA 71
           + +++ WSPP   ++K+N DAA  KE     +G++ RN++G++M   SK ++  L A  A
Sbjct: 85  MSKHKHWSPPPQDWYKVNVDAAVFKEQGTCGIGVVIRNYKGQIMGAMSKKVEFPLRALEA 144

Query: 72  QLWAILEGLKLVISLGCTMLVVESDCQLAINFINKVVDPLLP 114
           +  A   G+ L   LG   ++VE D QL I  +N V  P +P
Sbjct: 145 EAKAAEAGIFLAWDLGLKNIIVEGDAQLVIQALNGVAAPAIP 186

BLAST of Spg026251 vs. NCBI nr
Match: XP_024037110.1 (uncharacterized protein LOC112097050 [Citrus clementina] >XP_024037144.1 uncharacterized protein LOC112097050 [Citrus clementina] >XP_024037175.1 uncharacterized protein LOC112097050 [Citrus clementina])

HSP 1 Score: 67.0 bits (162), Expect = 2.3e-07
Identity = 37/89 (41.57%), Postives = 51/89 (57.30%), Query Frame = 0

Query: 18  WSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVMVVASKALDVRLEAHIAQLWAIL 77
           W PP+   +KLN+DAA  KE  +T LG I RN +G VM   +       +  IA+  AIL
Sbjct: 148 WHPPDPPRYKLNTDAALDKEGGVTGLGCIIRNHQGAVMAAVTSKKPYFGDVEIAEALAIL 207

Query: 78  EGLKLVISLGCTMLVVESDCQLAINFINK 107
           EGLKL   +  +  ++ESD +  +NFI K
Sbjct: 208 EGLKLAAEVALSPHLIESDSKNVVNFILK 236

BLAST of Spg026251 vs. NCBI nr
Match: XP_018815747.1 (uncharacterized protein LOC108987311 [Juglans regia] >KAF5465179.1 hypothetical protein F2P56_015208 [Juglans regia])

HSP 1 Score: 67.0 bits (162), Expect = 2.3e-07
Identity = 37/103 (35.92%), Postives = 60/103 (58.25%), Query Frame = 0

Query: 9   SSSLDRNRRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVMVVASKALDVRLEA 68
           S +LD N +W PP     KLN+D A   ++S + +G+I R+ +GKV++  S    V  + 
Sbjct: 73  SQALDMNYKWHPPPAAALKLNTDGAIFADLSSSGIGVILRDSQGKVLLSTSGREIVVQDP 132

Query: 69  HIAQLWAILEGLKLVISLGCTMLVVESDCQLAINFINKVVDPL 112
              +L  IL GL+L I +G + L+VESD  L++  I  + +P+
Sbjct: 133 IEVELLVILRGLQLCIPMGISKLIVESDSLLSVQAIQNMKEPM 175

BLAST of Spg026251 vs. ExPASy TrEMBL
Match: A0A1R3HQR7 (Ribonuclease H OS=Corchorus capsularis OX=210143 GN=CCACVL1_17615 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 7.7e-09
Identity = 39/89 (43.82%), Postives = 53/89 (59.55%), Query Frame = 0

Query: 17   RWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVMVVASKALDVRLEAHIAQLWAI 76
            RWSPP    WK+N+DAA  K++ +  LG + R+FEG+V+    K +    +   A L+AI
Sbjct: 1029 RWSPPAVGSWKINTDAAFDKDLGVAGLGAVLRDFEGRVLCSIIKKMHFIQDPLFAVLYAI 1088

Query: 77   LEGLKLVISLGCTMLVVESDCQLAINFIN 106
              GL+L  S G    V+ESDC LAI  IN
Sbjct: 1089 QLGLQLARSEGFLTCVIESDCLLAITAIN 1117

BLAST of Spg026251 vs. ExPASy TrEMBL
Match: A0A1S8ABJ9 (Ribonuclease H-like superfamily protein (Fragment) OS=Citrus limon OX=2708 PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.1e-07
Identity = 37/89 (41.57%), Postives = 51/89 (57.30%), Query Frame = 0

Query: 18  WSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVMVVASKALDVRLEAHIAQLWAIL 77
           W PP+   +KLN+DAA  KE  +T LG I RN +G VM   +       +  IA+  AIL
Sbjct: 148 WHPPDPPRYKLNTDAALDKEGGVTGLGCIIRNHQGAVMAAVTSKKPYFGDVEIAEALAIL 207

Query: 78  EGLKLVISLGCTMLVVESDCQLAINFINK 107
           EGLKL   +  +  ++ESD +  +NFI K
Sbjct: 208 EGLKLAAEVALSPHLIESDSKNVVNFILK 236

BLAST of Spg026251 vs. ExPASy TrEMBL
Match: A0A2I4E8M5 (uncharacterized protein LOC108987311 OS=Juglans regia OX=51240 GN=LOC108987311 PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.1e-07
Identity = 37/103 (35.92%), Postives = 60/103 (58.25%), Query Frame = 0

Query: 9   SSSLDRNRRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVMVVASKALDVRLEA 68
           S +LD N +W PP     KLN+D A   ++S + +G+I R+ +GKV++  S    V  + 
Sbjct: 73  SQALDMNYKWHPPPAAALKLNTDGAIFADLSSSGIGVILRDSQGKVLLSTSGREIVVQDP 132

Query: 69  HIAQLWAILEGLKLVISLGCTMLVVESDCQLAINFINKVVDPL 112
              +L  IL GL+L I +G + L+VESD  L++  I  + +P+
Sbjct: 133 IEVELLVILRGLQLCIPMGISKLIVESDSLLSVQAIQNMKEPM 175

BLAST of Spg026251 vs. ExPASy TrEMBL
Match: A0A6J1DJL6 (uncharacterized protein LOC111021711 OS=Momordica charantia OX=3673 GN=LOC111021711 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.4e-07
Identity = 39/105 (37.14%), Postives = 60/105 (57.14%), Query Frame = 0

Query: 6   NEDSSSLDRNRRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVMVVASKALDVR 65
           N + +  D N  W+PP+  + K+N D AC    S T +G++C+N +G+++V   + LDV 
Sbjct: 117 NSNKAGKDVN-YWAPPSPDWVKINVDTACKLSNSRTGIGVVCQNDKGEIIVAMIRKLDVF 176

Query: 66  LEAHIAQLWAILEGLKLVISLGCTMLVVESDCQLAINFINKVVDP 111
            E    +  AILEGL L   L    + V+SD Q +IN I++  DP
Sbjct: 177 YEPLQVETLAILEGLWLTQCLDLIFVQVDSDSQNSINLISQEYDP 220

BLAST of Spg026251 vs. ExPASy TrEMBL
Match: A0A6J1BQ49 (uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004786 PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 2.5e-07
Identity = 43/100 (43.00%), Postives = 55/100 (55.00%), Query Frame = 0

Query: 5   SNEDSSSLDRNRRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVMVVASKALDV 64
           S + SS    + RW PP     K+NSDAAC    + T LG+I R+  G ++V  S  L +
Sbjct: 102 SPQQSSVPPPSCRWLPPRDSAMKVNSDAACRS--TSTGLGLIIRDHFGVLLVAKSMFLPM 161

Query: 65  RLEAHIAQLWAILEGLKLVISLGCTMLVVESDCQLAINFI 105
            L    A++  ILE LKL  S   T LVVESDCQ AI  +
Sbjct: 162 PLNPLFAEIRGILEALKLAASRSYTRLVVESDCQEAIRLV 199

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_040996092.12.9e-1043.01LOW QUALITY PROTEIN: uncharacterized protein LOC121242268 [Juglans microcarpa x ... [more]
OMO72757.11.6e-0843.82hypothetical protein CCACVL1_17615 [Corchorus capsularis][more]
XP_023888246.17.9e-0835.29uncharacterized protein LOC112000364 [Quercus suber][more]
XP_024037110.12.3e-0741.57uncharacterized protein LOC112097050 [Citrus clementina] >XP_024037144.1 unchara... [more]
XP_018815747.12.3e-0735.92uncharacterized protein LOC108987311 [Juglans regia] >KAF5465179.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A1R3HQR77.7e-0943.82Ribonuclease H OS=Corchorus capsularis OX=210143 GN=CCACVL1_17615 PE=4 SV=1[more]
A0A1S8ABJ91.1e-0741.57Ribonuclease H-like superfamily protein (Fragment) OS=Citrus limon OX=2708 PE=4 ... [more]
A0A2I4E8M51.1e-0735.92uncharacterized protein LOC108987311 OS=Juglans regia OX=51240 GN=LOC108987311 P... [more]
A0A6J1DJL61.4e-0737.14uncharacterized protein LOC111021711 OS=Momordica charantia OX=3673 GN=LOC111021... [more]
A0A6J1BQ492.5e-0743.00uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 29..107
e-value: 1.9E-13
score: 50.3
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 25..148
e-value: 3.9E-9
score: 38.6
NoneNo IPR availablePANTHERPTHR47074BNAC02G40300D PROTEINcoord: 13..107
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 28..145
e-value: 2.92369E-12
score: 59.6352
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 26..107

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg026251.1Spg026251.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity