Spg025773 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg025773
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptioncytochrome P450 CYP749A22-like
Locationscaffold7: 3035176 .. 3037700 (+)
RNA-Seq ExpressionSpg025773
SyntenySpg025773
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCTTTCGGTTTCAGTGACACTACCGTCCTCCTCCTCGGCCTTCTCTGTGCTTCCATTATCTACCTATTCTTCAAGCTTTTTCTCAAGCTATGCTGGAAACCTTTTCGGATTCGGCGTTTTATGCGATCGCAGGGGATTCAGGGTCCTCCTTCCAGCTTCATCTATGGAAACCTCTTTCAAATCATGAAGATGAAGATGGAATCCATGCGCACCTCTCTCGATATCACCGACCATAACATACTTCCCAGAGTTCATCCTCATGTTCACTTGTGGATCAACAAATATGGTTCGTCTTCACTCTCCGAGTCCGAGCTTCAGCTTAATTTCATTGTTTTCGGTTTGATTGACTGATTTGAGTTACAGGGAAAAACTTCCTTCAGTGGAATGGGAATATGGCTCAGTTGATCGTTTCGGATCCGGAGACGATCAAGGAGATACTTCATAATCAAGAACGAGCTATTTTCAAAATGACTCTCAACGAAGATTCTCGGAGGATATTCGGGAATGGACAGTTGACTTCCGAAGGAGAAAAATGGGCCAAGATTCGAAGAATTGCTGATTATGCTTTCCATGGGGAACTCCTAAAAGTTAGTGGGATTTGGTTCTGTTTAATGATTTTACTGATTCAGTTGTGCCTTTAGATTCAATTTCTTGAAAATGGTTTTGCAGAAGATGGGGCCAACCATGGTTTCTTGTGCTGAGGCGATGGTGGAAAAGTGGAAGAAGCAACCTGAAGGCAAAGAGGTTGATTTGTTTGAAGAGTTTAAGGTGTTAACTTCAGATATCATTGCACATACAGCCTTTGGAAGCAGTTATTTGGAAGGGAAATTCATTTTTGAGACTCTGACTAAACTGGCCATGATATTTTTTAAGAATCAATTCAAACGAGGCATTCCTGGAATCAGGTGTTCTTTTAAGTATTTTTGAAACAATCTGCTTACTTGCATTGATGCTATCTAAGTAAAACTAAATTATTAAGTTTGTGTGCAGCAAGTTATTCAAATCAAAGGATGCTAGGGAGGGAGATGAGCTAGCAAGAAAGATGCAAAGCTCCATAATTTCAATAATAAAAAATAGAGAAGAGAAGGTGATAAGTGGTGAAGCAGAGAACTATGGTAATGATTTTCTTGCATTACTTTTGAAGGCAAAGAATAATTCTGACCAGAGGCAGAGGATTTCTGTTGAGGATGTGGTAGATGAATGCAAAGCAATGTACTTTTCGGGGCAAGAAACTACGGGTGTTTTGCTTTCTTGGACTGCGTTTCTGTTGGCAACTCATGTGCACTGGCAAGAGGAAGCAAGAAAGGAAGTGTTGGATATGTTTGGCAACGATAATCCAACTTTAGAGGGCATCACAAAGTTAAAGATTGTAAGCATGTCTCTCCATTATTAAGGCCATTACTTTTTCATAGAGTAGGCTTAAGATTTGTTGGTGTAGCAGAACTCCGAAAAGGGCCATGATGGATGATAAACATCCAATTCCCATATGCTTGATTTAGTAGTCTTTCATTAAGGGTTTAGATTGAGATTTTGTAATTTATCTTTATCTAGAATAGAGATTTTAGTTCAGGTTTTTCCAGTCTTATTTTAGATTTACACTGTTCAAATCTGCAATTGGATTCAGGAAGGTTTGGTTTAAACTGCCATTCTTGGAAAATGAAAATCTTCCCAACTTTCTATAGGCTAACATGAGAATCTGCCAACCATAGCTTAGTTAGTTAAAACATATACTTTGAGGTTCGAAAACTTCATCCCCATTTCCTCTCTTTTACAATGACTGCTTTTCTTCCATTATGAATCAAGAACTGAGAGTTGTTTTCACTCTTGTCTTATAGATGAGCATGATCATCAAGGAAACGTTAAGATTATATCCTCCAACCCAAGTCATGTCAAGGTTGGTTAAGAAGGGAGTCAGACTGGGGAAGCTCGTTCTCCCAGCCAACATTCAAGTTAGCATCTCAACGATTGCAGTTCATCATGATACTGCAATATGGGGTGAAGATGCTCATGAGTTCAAACCAGAAAGATTTTCAGAAGGAAAAGCTGAAGATATCCCATCAGGTGCATACATACCATTTGGGTTGGGTCCTCGAAACTGCGTTGGCAATATGTTGGCCATCAACGAAACTAAGATTACATTATCGATGATTCTACAACGATTTTCTTTCACCATTTCCCCAGGCCACGTCCACGCACCGTACCAGTTCCTCACCAATTGCCCGCAACATGGAGTTCGGGTGAAGCTTCAGTCTCTGACTGGATGAAAGGGGAAGGAAGATGTATAATTGTATATGGTTTAACTGCCCACTCAGACACCTTCCATTGTCGGGCCCTTTTTTAGGTTTTGGTAAATTAAACCTTTGAATCAATTCATCCTCTCTTTATGTTGTTTCACTATATTTAGTCTTAGTTTTGTATTTTGTCCATGTACTCTATTGAGTTGTGTTTCATGTTCCTTCGTCCTTTTCTTCTATTAGCTCAACGACTAAAATATCAATAATAGTTTTCTTGAGTTTGAG

mRNA sequence

ATGGCCGCTTTCGGTTTCAGTGACACTACCGTCCTCCTCCTCGGCCTTCTCTGTGCTTCCATTATCTACCTATTCTTCAAGCTTTTTCTCAAGCTATGCTGGAAACCTTTTCGGATTCGGCGTTTTATGCGATCGCAGGGGATTCAGGGTCCTCCTTCCAGCTTCATCTATGGAAACCTCTTTCAAATCATGAAGATGAAGATGGAATCCATGCGCACCTCTCTCGATATCACCGACCATAACATACTTCCCAGAGTTCATCCTCATTGGAATGGGAATATGGCTCAGTTGATCGTTTCGGATCCGGAGACGATCAAGGAGATACTTCATAATCAAGAACGAGCTATTTTCAAAATGACTCTCAACGAAGATTCTCGGAGGATATTCGGGAATGGACAGTTGACTTCCGAAGGAGAAAAATGGGCCAAGATTCGAAGAATTGCTGATTATGCTTTCCATGGGGAACTCCTAAAAAAGATGGGGCCAACCATGGTTTCTTGTGCTGAGGCGATGGTGGAAAAGTGGAAGAAGCAACCTGAAGGCAAAGAGGTTGATTTGTTTGAAGAGTTTAAGGTGTTAACTTCAGATATCATTGCACATACAGCCTTTGGAAGCAGTTATTTGGAAGGGAAATTCATTTTTGAGACTCTGACTAAACTGGCCATGATATTTTTTAAGAATCAATTCAAACGAGGCATTCCTGGAATCAGCAAGTTATTCAAATCAAAGGATGCTAGGGAGGGAGATGAGCTAGCAAGAAAGATGCAAAGCTCCATAATTTCAATAATAAAAAATAGAGAAGAGAAGGTGATAAGTGGTGAAGCAGAGAACTATGGTAATGATTTTCTTGCATTACTTTTGAAGGCAAAGAATAATTCTGACCAGAGGCAGAGGATTTCTGTTGAGGATGTGGTAGATGAATGCAAAGCAATGTACTTTTCGGGGCAAGAAACTACGGGTGTTTTGCTTTCTTGGACTGCGTTTCTGTTGGCAACTCATGTGCACTGGCAAGAGGAAGCAAGAAAGGAAGTGTTGGATATGTTTGGCAACGATAATCCAACTTTAGAGGGCATCACAAAGTTAAAGATTGTAAGCATGTCTCTCCATTATTAA

Coding sequence (CDS)

ATGGCCGCTTTCGGTTTCAGTGACACTACCGTCCTCCTCCTCGGCCTTCTCTGTGCTTCCATTATCTACCTATTCTTCAAGCTTTTTCTCAAGCTATGCTGGAAACCTTTTCGGATTCGGCGTTTTATGCGATCGCAGGGGATTCAGGGTCCTCCTTCCAGCTTCATCTATGGAAACCTCTTTCAAATCATGAAGATGAAGATGGAATCCATGCGCACCTCTCTCGATATCACCGACCATAACATACTTCCCAGAGTTCATCCTCATTGGAATGGGAATATGGCTCAGTTGATCGTTTCGGATCCGGAGACGATCAAGGAGATACTTCATAATCAAGAACGAGCTATTTTCAAAATGACTCTCAACGAAGATTCTCGGAGGATATTCGGGAATGGACAGTTGACTTCCGAAGGAGAAAAATGGGCCAAGATTCGAAGAATTGCTGATTATGCTTTCCATGGGGAACTCCTAAAAAAGATGGGGCCAACCATGGTTTCTTGTGCTGAGGCGATGGTGGAAAAGTGGAAGAAGCAACCTGAAGGCAAAGAGGTTGATTTGTTTGAAGAGTTTAAGGTGTTAACTTCAGATATCATTGCACATACAGCCTTTGGAAGCAGTTATTTGGAAGGGAAATTCATTTTTGAGACTCTGACTAAACTGGCCATGATATTTTTTAAGAATCAATTCAAACGAGGCATTCCTGGAATCAGCAAGTTATTCAAATCAAAGGATGCTAGGGAGGGAGATGAGCTAGCAAGAAAGATGCAAAGCTCCATAATTTCAATAATAAAAAATAGAGAAGAGAAGGTGATAAGTGGTGAAGCAGAGAACTATGGTAATGATTTTCTTGCATTACTTTTGAAGGCAAAGAATAATTCTGACCAGAGGCAGAGGATTTCTGTTGAGGATGTGGTAGATGAATGCAAAGCAATGTACTTTTCGGGGCAAGAAACTACGGGTGTTTTGCTTTCTTGGACTGCGTTTCTGTTGGCAACTCATGTGCACTGGCAAGAGGAAGCAAGAAAGGAAGTGTTGGATATGTTTGGCAACGATAATCCAACTTTAGAGGGCATCACAAAGTTAAAGATTGTAAGCATGTCTCTCCATTATTAA

Protein sequence

MAAFGFSDTTVLLLGLLCASIIYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLDITDHNILPRVHPHWNGNMAQLIVSDPETIKEILHNQERAIFKMTLNEDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFKSKDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVEDVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLKIVSMSLHY
Homology
BLAST of Spg025773 vs. NCBI nr
Match: XP_022149008.1 (cytochrome P450 CYP749A22-like [Momordica charantia])

HSP 1 Score: 558.9 bits (1439), Expect = 3.2e-155
Identity = 283/372 (76.08%), Postives = 315/372 (84.68%), Query Frame = 0

Query: 9   TTVLLLGLLCASIIYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKM 68
           T V LLG+L AS I   F + LKL WKP RI+R MRSQG+ GPPS+FIYGN+ Q+MKMK 
Sbjct: 5   TAVFLLGVLSASSI---FYVLLKLWWKPLRIQRIMRSQGVYGPPSNFIYGNMIQVMKMKF 64

Query: 69  ESMRTSLDITDHNILPRVHPH--------------WNGNMAQLIVSDPETIKEILHNQER 128
           ESMRTSLDI+DH+ILPRV+PH              WNG  AQLIVSDPETIKEILHN+ER
Sbjct: 65  ESMRTSLDISDHHILPRVNPHIHLWIDKYGKNFIQWNGTQAQLIVSDPETIKEILHNRER 124

Query: 129 AIFKMTLNEDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEK 188
           AI K+ L  D+RRIFG+G  TS+GEKWAK RRIADYAFHGELLK M PTMVSCAEAMVEK
Sbjct: 125 AITKIDLGGDARRIFGDGLSTSDGEKWAKARRIADYAFHGELLKNMMPTMVSCAEAMVEK 184

Query: 189 WKKQPEGKEVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIP 248
           WKK  EGKE+DLFEEFK+LTSDIIAHTAFGSSYLEGKFIFETL+KL+MI FKNQFKR IP
Sbjct: 185 WKKNDEGKELDLFEEFKLLTSDIIAHTAFGSSYLEGKFIFETLSKLSMILFKNQFKRRIP 244

Query: 249 GISKLFKSKDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSD 308
           G SKLFK+KDAREGDEL  +M++SI+SIIK REEKV+SGEA+NYGNDFL LLLKAKN+ D
Sbjct: 245 GFSKLFKTKDAREGDELEMRMKNSIVSIIKKREEKVMSGEADNYGNDFLGLLLKAKNDPD 304

Query: 309 QRQRISVEDVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPT 367
            RQRISV+DVVDECK +YF+GQETT VLLSWT+FLLATHV WQEEARKEV DMFGN NPT
Sbjct: 305 PRQRISVDDVVDECKTVYFAGQETTNVLLSWTSFLLATHVDWQEEARKEVFDMFGNTNPT 364

BLAST of Spg025773 vs. NCBI nr
Match: XP_022149052.1 (cytochrome P450 CYP749A22-like [Momordica charantia] >XP_022149053.1 cytochrome P450 CYP749A22-like [Momordica charantia])

HSP 1 Score: 383.3 bits (983), Expect = 2.4e-102
Identity = 189/363 (52.07%), Postives = 258/363 (71.07%), Query Frame = 0

Query: 21  IIYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLDITDH 80
           +++   KLF+KL W P RI+RFMRSQGIQGP  +FI GN  ++   +  ++ T +D++ H
Sbjct: 17  LLFGTIKLFIKLWWTPIRIQRFMRSQGIQGPSYNFIQGNAREMHTRRTHAIATPIDLS-H 76

Query: 81  NILPRVHPH--------------WNGNMAQLIVSDPETIKEILHNQERAIFKMTLNEDSR 140
            ILPRV PH              W G  AQLI++DPE I+E+LH++++   K  L+    
Sbjct: 77  RILPRVLPHTHSWLHRYGRNFIQWFGVEAQLIITDPEMIREVLHDRQKKFPKAKLHGFLN 136

Query: 141 RIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDL 200
           +IFGNG +T+EGE WAK R+IA++AFHG+ LK M P M+ CAE M+E+WK   EGKE+D+
Sbjct: 137 KIFGNGLVTAEGETWAKSRKIANFAFHGDTLKNMIPAMIGCAETMMEEWKHY-EGKELDV 196

Query: 201 FEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFKSKDAR 260
           F+  KV TSD+I+HTAFGSSY +GK IF+ L  L  +  +N FK  +PGI K+FKSKD  
Sbjct: 197 FQHLKVYTSDVISHTAFGSSYEQGKNIFQMLQNLCELIIRNGFKIRLPGIRKIFKSKDDV 256

Query: 261 EGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVEDVVD 320
           EG+ L ++M+   + +IK+REEK I+GEA+ YGNDFL LL+KAKN+ ++ QRISV+D+VD
Sbjct: 257 EGERLEKRMKDCFMEVIKSREEKFINGEADGYGNDFLGLLVKAKNDPEESQRISVDDIVD 316

Query: 321 ECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLKIVSM 370
           ECK  YF+G ETT VLL+WT FLLA H  WQ++AR EV  +FG+ NPT EG+ KLK ++M
Sbjct: 317 ECKTFYFAGHETTSVLLAWTMFLLALHKQWQDQARDEVFKVFGDKNPTSEGLPKLKTMTM 376

BLAST of Spg025773 vs. NCBI nr
Match: XP_038892924.1 (cytochrome P450 CYP749A22-like isoform X1 [Benincasa hispida])

HSP 1 Score: 382.5 bits (981), Expect = 4.2e-102
Identity = 194/367 (52.86%), Postives = 256/367 (69.75%), Query Frame = 0

Query: 17  LCASIIYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLD 76
           L   +++   KLF+KL W P RI+RFMRSQGIQGP   FI GN   +   +M++M T +D
Sbjct: 13  LFGCLLFGVIKLFVKLWWTPMRIQRFMRSQGIQGPSYKFIQGNTRDVYTKRMQAMATPMD 72

Query: 77  ITDHNILPRVHP--------------HWNGNMAQLIVSDPETIKEILHNQERAIFKMTLN 136
           ++ H+ILPRV P               W G  AQLI++DPE IKE+LH++++   K  L 
Sbjct: 73  LS-HHILPRVMPSVHSWLNLYGRNFLQWFGVDAQLIITDPEMIKEVLHDRQKNFPKAKLQ 132

Query: 137 EDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK 196
               RIFGNG  T+EGE+WAK R+IA++AFHG+ LK M PTM+ C E M E WK   EGK
Sbjct: 133 GHIHRIFGNGLATAEGERWAKSRKIANFAFHGDSLKNMIPTMIECGEKMTEGWKNY-EGK 192

Query: 197 EVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFKS 256
           E+D+F+EFKV T D+I+HTAFGSSY +GK IF  L +L  +  +N +K  +P ISK+ KS
Sbjct: 193 ELDVFKEFKVYTLDVISHTAFGSSYQQGKDIFHMLQELCELSIRNGYKIKLPIISKILKS 252

Query: 257 KDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVE 316
           KD  EG+ L ++M+   + IIK REEK+ +GEA+ YGNDFL LL+KAKN+ +  QRISVE
Sbjct: 253 KDDYEGERLEKRMKDCFMEIIKGREEKLRNGEADGYGNDFLGLLVKAKNDPEISQRISVE 312

Query: 317 DVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLK 370
           D+VDECK  YF+G ETT VLL+WT FLLA H  WQEEAR EVL +FG+ NPTLEG+ KL+
Sbjct: 313 DIVDECKTFYFAGHETTNVLLAWTMFLLALHKEWQEEARNEVLKVFGDKNPTLEGLPKLR 372

BLAST of Spg025773 vs. NCBI nr
Match: XP_004135430.2 (cytochrome P450 CYP749A22 isoform X1 [Cucumis sativus] >KAE8648770.1 hypothetical protein Csa_008223 [Cucumis sativus])

HSP 1 Score: 381.3 bits (978), Expect = 9.3e-102
Identity = 191/367 (52.04%), Postives = 257/367 (70.03%), Query Frame = 0

Query: 17  LCASIIYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLD 76
           L   +++  FKLF+KL W P RI+RFMRSQGIQGP   FI GN   +   +M++M T +D
Sbjct: 13  LSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVYIKRMQAMATPMD 72

Query: 77  ITDHNILPRVHP--------------HWNGNMAQLIVSDPETIKEILHNQERAIFKMTLN 136
           ++ HNIL RV P               W+G  AQL+++DPE IKE+LH+++++  K  L 
Sbjct: 73  LS-HNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHDRQKSFPKAKLK 132

Query: 137 EDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK 196
               RIFGNG  T+EG++WA  RRIA++AFHG+ LK M PTM+ C E M+E WK   EGK
Sbjct: 133 GHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNY-EGK 192

Query: 197 EVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFKS 256
           E+D F+EFKV T D+I+HTAFGSSY +GK IF  L +L  +  +N +K  +P ISK+ KS
Sbjct: 193 ELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIRLPIISKILKS 252

Query: 257 KDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVE 316
           KD  EG+ L ++++   + IIK REEK+ +GEA +YGNDFL LL+KAKN+ +  Q IS+E
Sbjct: 253 KDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKNDPETSQCISME 312

Query: 317 DVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLK 370
           D+VDECK  YF+G ETT VLL+WT  LLA H  WQE+AR EVLD+FG++NPTLEG+ KLK
Sbjct: 313 DIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNNPTLEGLPKLK 372

BLAST of Spg025773 vs. NCBI nr
Match: XP_008446466.1 (PREDICTED: cytochrome P450 CYP749A22-like [Cucumis melo])

HSP 1 Score: 377.9 bits (969), Expect = 1.0e-100
Identity = 188/367 (51.23%), Postives = 255/367 (69.48%), Query Frame = 0

Query: 17  LCASIIYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLD 76
           L   ++++ FKLF+KL W P RI+RFMRSQGIQGP   FI GN   +   +M++M T +D
Sbjct: 13  LSGCLLFVVFKLFVKLWWTPMRIQRFMRSQGIQGPSYKFIQGNTRDVYMKRMQAMATPMD 72

Query: 77  ITDHNILPRVHP--------------HWNGNMAQLIVSDPETIKEILHNQERAIFKMTLN 136
           ++ HNILPRV P               W G  AQL+++DPE IKE+LH+++++  K  L 
Sbjct: 73  LS-HNILPRVMPSVHSWLNLYGRNYLQWAGVDAQLMITDPEMIKEVLHDRQKSFPKAKLK 132

Query: 137 EDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK 196
               RIFGNG  T+EG++W   RRIA++AFHG+ LK M PTM+ C E M+E WK   EGK
Sbjct: 133 GHIDRIFGNGLATAEGQRWVNSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNY-EGK 192

Query: 197 EVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFKS 256
           E+D F+EFKV T D+I+HTAFGSSY +GK IF  L +L  +  +N +K  +P ISK+ KS
Sbjct: 193 ELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLQELCDLSIRNGYKIKLPIISKILKS 252

Query: 257 KDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVE 316
           KD  EG+ L ++++   + IIK REEK+ + EA +YGNDFL LL+KAKN+ +  Q IS+E
Sbjct: 253 KDDYEGERLEKRLKDCFMEIIKEREEKLRNDEASDYGNDFLGLLIKAKNDPETSQSISME 312

Query: 317 DVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLK 370
           D+VDECK  YF+G ETT VLL+WT  LLA H  WQE+AR EV D+FG+++PTLEG+ KLK
Sbjct: 313 DIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVFDVFGHNHPTLEGLPKLK 372

BLAST of Spg025773 vs. ExPASy Swiss-Prot
Match: H2DH17 (Cytochrome P450 CYP749A22 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 5.9e-91
Identity = 165/372 (44.35%), Postives = 249/372 (66.94%), Query Frame = 0

Query: 13  LLGLLCASIIYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMR 72
           LL  LC  ++++F +    + W P R++R  R QGI+GP   F+YGN  +I+ M+ ESM 
Sbjct: 19  LLSSLCVFLLFVFIRFLNDIWWTPIRLQRVFRQQGIRGPSYGFLYGNTKEILNMRKESMS 78

Query: 73  TSLDITDHNILPRVHPH--------------WNGNMAQLIVSDPETIKEILHNQERAIFK 132
             +D   HNI PR+ PH              W G  AQ +V+  + +KE +  +++A  K
Sbjct: 79  RPMDYLSHNIFPRLQPHLYSWLNIYGKNFLNWYGPRAQFVVTQVDFVKETM-IKDQAYPK 138

Query: 133 MTLNEDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQ 192
           M     ++++ G+G +TS+G+KWAK RR+A++AFH E LK M P M++  E M+++W KQ
Sbjct: 139 MDPEWFAKKLLGDGIVTSKGKKWAKHRRLANHAFHAESLKSMTPAMIASVEMMLKRW-KQ 198

Query: 193 PEGKEVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISK 252
            EG+E+D+F+EFK+LTS++I+ TAFGSSYL+GK IF+ LT+L +I  +N +K  +PGIS 
Sbjct: 199 HEGREIDVFQEFKILTSEVISRTAFGSSYLDGKDIFDRLTQLGIIITRNSYKVKLPGISL 258

Query: 253 LFKSKDAREGDELARKMQSSIISIIKNRE-EKVISGEAENYGNDFLALLLKAKNNSDQRQ 312
            +KS D  E ++L + +  SI+ I++ RE E  +SGE  ++G DFL LL+KA N++D++ 
Sbjct: 259 FYKSNDEIEAEKLDQGLYDSILRIMEKREKESTMSGEVGSFGTDFLGLLMKAMNDADEKN 318

Query: 313 RISVEDVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEG 370
           RI+ +DVVDECK  Y +GQETT  LL+W  FLL  H  WQE+AR+EVL++FG + P  +G
Sbjct: 319 RITAQDVVDECKTFYVAGQETTTTLLAWVIFLLGIHTDWQEKARQEVLNLFGQEIPNSDG 378

BLAST of Spg025773 vs. ExPASy Swiss-Prot
Match: Q9ASR3 (Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.6e-56
Identity = 136/381 (35.70%), Postives = 209/381 (54.86%), Query Frame = 0

Query: 11  VLLLGLLCASII--YLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKM 70
           V+ L L+   +I     FK F+ L W PF + R +++QGI GP     YGNL +I KMK 
Sbjct: 4   VIFLALIVLILIIGLRIFKAFMILVWHPFVLTRRLKNQGISGPNYRIFYGNLSEIKKMKR 63

Query: 71  ESMRTSLDITDHNILPRVHPH--------------WNGNMAQLIVSDPETIKEILHNQER 130
           ES  + LD + ++I PR+ PH              WNG   ++ +SDPE  K +L N+  
Sbjct: 64  ESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTMLSNKLG 123

Query: 131 AIFKMTLNEDSRRIFGN-GQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVE 190
              K     ++ ++ G+ G +  EG  W + RRI + AF  + LK M   MV C   M+E
Sbjct: 124 FFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVDCTLKMLE 183

Query: 191 KWKKQPEGKEV-------DLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFK 250
           +W+K+   +E        ++ EEF+ LT+DIIA +AFGSSY+EG  +F +  +L   +  
Sbjct: 184 EWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELKRCYTT 243

Query: 251 NQFKRGIPGISKLFKSKDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALL 310
           +  +  IPG   L    + R   +L RKM +SI  II +R +        +YG+D L +L
Sbjct: 244 SLNQVSIPGTQYLPTPSNIRVW-KLERKMDNSIKRIISSRLQ-----SKSDYGDDLLGIL 303

Query: 311 LKAKNNSDQRQRISVEDVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLD 367
           LKA N   + +++S+E+++ EC+  +F G ETT  LL+WT  LL+ H  WQE+ R+E+  
Sbjct: 304 LKAYNTEGKERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKLREEIFK 363

BLAST of Spg025773 vs. ExPASy Swiss-Prot
Match: F4IK45 (Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.6e-54
Identity = 128/353 (36.26%), Postives = 192/353 (54.39%), Query Frame = 0

Query: 32  LCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLDITDHNILPRVHPH-- 91
           L W+P+ + R  + QGI GP    +YGNL +I KMK E+    LD   ++I+PRV PH  
Sbjct: 84  LVWRPWMLSRRFKKQGISGPKYRILYGNLREIRKMKNEAKLMVLDPNSNDIVPRVLPHLQ 143

Query: 92  ------------WNGNMAQLIVSDPETIKEILHNQERAIFKMTLNEDSRRIFGNGQLTSE 151
                       W G   +L +SD E  K+IL N+     K     +  ++ GNG +   
Sbjct: 144 QWKSQYGETFLYWQGTDPRLCISDHELAKQILSNKFVFFSKSKTKPEILKLSGNGLIFVN 203

Query: 152 GEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDLF----EEFKVL 211
           G  W + RRI + AF  + LK M   MV C   M  +WKKQ  G E + F     EFK L
Sbjct: 204 GLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFRMFLEWKKQRNGVETEQFVLISREFKRL 263

Query: 212 TSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFKSKDAREGDELAR 271
           T+DIIA  AFGSSY EG  +F++  +L             PGI  L    + +   +L  
Sbjct: 264 TADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGIQYLPTPSNLQIW-KLDM 323

Query: 272 KMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVEDVVDECKAMYF 331
           K+ SSI  II  R    ++ E+++YGND L ++L A ++++  +++S++++++ECK  +F
Sbjct: 324 KVNSSIKRIIDAR----LTSESKDYGNDLLGIMLTAASSNESEKKMSIDEIIEECKTFFF 383

Query: 332 SGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDN-PTLEGITKLKIVS 366
           +G ETT  LL+W+  LL+ H  WQE+ R+EV +  G D  P  E  +KLK+++
Sbjct: 384 AGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPDAETCSKLKLMN 431

BLAST of Spg025773 vs. ExPASy Swiss-Prot
Match: Q2MJ21 (Cytochrome P450 716A67 OS=Medicago truncatula OX=3880 GN=CYP72A67 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.6e-51
Identity = 127/361 (35.18%), Postives = 197/361 (54.57%), Query Frame = 0

Query: 35  KPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLDITDHNILPRVHPH----- 94
           KP R+ + +R QG  G     + G+L    KM  ++    ++++D +I+PRV P+     
Sbjct: 33  KPKRLEKLLREQGCNGNSYRLVLGDLKDSYKMGKKAKSKPMELSD-DIIPRVIPYIQQLV 92

Query: 95  ---------WNGNMAQLIVSDPETIKEILHNQERAIFKMTLNEDSRRIFG---NGQLTSE 154
                    W+G   +LI+++PE IK++L+          L +    IF    +G +  E
Sbjct: 93  QIYGKNPFIWSGTTPRLILTEPELIKDVLNRTSE------LQKPKYEIFKFLFSGLIIHE 152

Query: 155 GEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKK--QPEGK-EVDLFEEFKVLT 214
           GEKW K RR+ + AF  E LK M P+ ++    M+ KW+     +G  E+D++   + LT
Sbjct: 153 GEKWRKHRRLMNAAFQLEKLKIMAPSFLTSCIDMISKWESTLSSDGSGEIDIWPSLQNLT 212

Query: 215 SDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFKSKDAREGDELARK 274
           SD+I+  AFGSSY EGK IF+   +   +  KN  K  IP + +   +   R   E+ + 
Sbjct: 213 SDVISRNAFGSSYEEGKRIFDLQREQGELVMKNLVKSLIP-LWRFIPTATQRRMHEIEKD 272

Query: 275 MQSSIISIIKNREEKVISGEAENYGNDFLALLLKAK------NNSDQRQRISVEDVVDEC 334
           + SS+  II  RE+ + +GEA    ND L LLL++       + +++   +S+EDVV EC
Sbjct: 273 IDSSLRYIINKREKAMKAGEATE--NDLLGLLLESNHQEIRDHGNNKNMGMSLEDVVGEC 332

Query: 335 KAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLKIVSMSL 370
           K  Y +GQE+T  +L WT  LL+ +  WQE AR+EVL +FGN  P  EG+ KLKI+ M L
Sbjct: 333 KLFYLAGQESTSTMLVWTMILLSRYPDWQERAREEVLQIFGNKKPDYEGLNKLKILPMIL 383

BLAST of Spg025773 vs. ExPASy Swiss-Prot
Match: Q2MJ19 (Cytochrome P450 72A68 OS=Medicago truncatula OX=3880 GN=CYP72A68 PE=1 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 3.5e-51
Identity = 119/358 (33.24%), Postives = 200/358 (55.87%), Query Frame = 0

Query: 35  KPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLDITDHNILPRVHPH----- 94
           KP +I + +R QG+QG P   + G+      M+ +     ++++D +I PRV P+     
Sbjct: 33  KPKKIEKLLREQGLQGNPYRLLLGDAKDYFVMQKKVQSKPMNLSD-DIAPRVAPYIHHAV 92

Query: 95  ---------WNGNMAQLIVSDPETIKEILHNQERAIFKMTLNEDSRRIFGNGQLTSEGEK 154
                    W G    +I+++PE I+E+ +          +     ++   G +  EGEK
Sbjct: 93  QTHGKKSFIWFGMKPWVILNEPEQIREVFNKMSEF---PKVQYKFMKLITRGLVKLEGEK 152

Query: 155 WAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK---EVDLFEEFKVLTSDI 214
           W+K RRI + AFH E LK M PT +     ++  W+K        E+D++   + LTSD+
Sbjct: 153 WSKHRRIINPAFHMEKLKIMTPTFLKSCNDLISNWEKMLSSNGSCEMDVWPSLQSLTSDV 212

Query: 215 IAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFKSKDAREGDELARKMQS 274
           IA ++FGSSY EG+ +F+   +   +  KN  K  IP + +   + D R+ +E  +++++
Sbjct: 213 IARSSFGSSYEEGRKVFQLQIEQGELIMKNLMKSLIP-LWRFLPTADHRKINENEKQIET 272

Query: 275 SIISIIKNREEKVISGEAENYGNDFLALLLKA------KNNSDQRQRISVEDVVDECKAM 334
           ++ +II  RE+ + +GEA    ND L LLL++      ++ + +   +S+E+VV EC+  
Sbjct: 273 TLKNIINKREKAIKAGEATE--NDLLGLLLESNHREIKEHGNVKNMGLSLEEVVGECRLF 332

Query: 335 YFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLKIVSMSLH 370
           + +GQETT  LL WT  LL+ +  WQE ARKEVL++FGN+ P  +G+ KLKI++M L+
Sbjct: 333 HVAGQETTSDLLVWTMVLLSRYPDWQERARKEVLEIFGNEKPDFDGLNKLKIMAMILY 383

BLAST of Spg025773 vs. ExPASy TrEMBL
Match: A0A6J1D5N2 (cytochrome P450 CYP749A22-like OS=Momordica charantia OX=3673 GN=LOC111017532 PE=3 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.6e-155
Identity = 283/372 (76.08%), Postives = 315/372 (84.68%), Query Frame = 0

Query: 9   TTVLLLGLLCASIIYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKM 68
           T V LLG+L AS I   F + LKL WKP RI+R MRSQG+ GPPS+FIYGN+ Q+MKMK 
Sbjct: 5   TAVFLLGVLSASSI---FYVLLKLWWKPLRIQRIMRSQGVYGPPSNFIYGNMIQVMKMKF 64

Query: 69  ESMRTSLDITDHNILPRVHPH--------------WNGNMAQLIVSDPETIKEILHNQER 128
           ESMRTSLDI+DH+ILPRV+PH              WNG  AQLIVSDPETIKEILHN+ER
Sbjct: 65  ESMRTSLDISDHHILPRVNPHIHLWIDKYGKNFIQWNGTQAQLIVSDPETIKEILHNRER 124

Query: 129 AIFKMTLNEDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEK 188
           AI K+ L  D+RRIFG+G  TS+GEKWAK RRIADYAFHGELLK M PTMVSCAEAMVEK
Sbjct: 125 AITKIDLGGDARRIFGDGLSTSDGEKWAKARRIADYAFHGELLKNMMPTMVSCAEAMVEK 184

Query: 189 WKKQPEGKEVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIP 248
           WKK  EGKE+DLFEEFK+LTSDIIAHTAFGSSYLEGKFIFETL+KL+MI FKNQFKR IP
Sbjct: 185 WKKNDEGKELDLFEEFKLLTSDIIAHTAFGSSYLEGKFIFETLSKLSMILFKNQFKRRIP 244

Query: 249 GISKLFKSKDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSD 308
           G SKLFK+KDAREGDEL  +M++SI+SIIK REEKV+SGEA+NYGNDFL LLLKAKN+ D
Sbjct: 245 GFSKLFKTKDAREGDELEMRMKNSIVSIIKKREEKVMSGEADNYGNDFLGLLLKAKNDPD 304

Query: 309 QRQRISVEDVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPT 367
            RQRISV+DVVDECK +YF+GQETT VLLSWT+FLLATHV WQEEARKEV DMFGN NPT
Sbjct: 305 PRQRISVDDVVDECKTVYFAGQETTNVLLSWTSFLLATHVDWQEEARKEVFDMFGNTNPT 364

BLAST of Spg025773 vs. ExPASy TrEMBL
Match: A0A6J1D6R8 (cytochrome P450 CYP749A22-like OS=Momordica charantia OX=3673 GN=LOC111017564 PE=3 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 1.2e-102
Identity = 189/363 (52.07%), Postives = 258/363 (71.07%), Query Frame = 0

Query: 21  IIYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLDITDH 80
           +++   KLF+KL W P RI+RFMRSQGIQGP  +FI GN  ++   +  ++ T +D++ H
Sbjct: 17  LLFGTIKLFIKLWWTPIRIQRFMRSQGIQGPSYNFIQGNAREMHTRRTHAIATPIDLS-H 76

Query: 81  NILPRVHPH--------------WNGNMAQLIVSDPETIKEILHNQERAIFKMTLNEDSR 140
            ILPRV PH              W G  AQLI++DPE I+E+LH++++   K  L+    
Sbjct: 77  RILPRVLPHTHSWLHRYGRNFIQWFGVEAQLIITDPEMIREVLHDRQKKFPKAKLHGFLN 136

Query: 141 RIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDL 200
           +IFGNG +T+EGE WAK R+IA++AFHG+ LK M P M+ CAE M+E+WK   EGKE+D+
Sbjct: 137 KIFGNGLVTAEGETWAKSRKIANFAFHGDTLKNMIPAMIGCAETMMEEWKHY-EGKELDV 196

Query: 201 FEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFKSKDAR 260
           F+  KV TSD+I+HTAFGSSY +GK IF+ L  L  +  +N FK  +PGI K+FKSKD  
Sbjct: 197 FQHLKVYTSDVISHTAFGSSYEQGKNIFQMLQNLCELIIRNGFKIRLPGIRKIFKSKDDV 256

Query: 261 EGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVEDVVD 320
           EG+ L ++M+   + +IK+REEK I+GEA+ YGNDFL LL+KAKN+ ++ QRISV+D+VD
Sbjct: 257 EGERLEKRMKDCFMEVIKSREEKFINGEADGYGNDFLGLLVKAKNDPEESQRISVDDIVD 316

Query: 321 ECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLKIVSM 370
           ECK  YF+G ETT VLL+WT FLLA H  WQ++AR EV  +FG+ NPT EG+ KLK ++M
Sbjct: 317 ECKTFYFAGHETTSVLLAWTMFLLALHKQWQDQARDEVFKVFGDKNPTSEGLPKLKTMTM 376

BLAST of Spg025773 vs. ExPASy TrEMBL
Match: A0A0A0KQY3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G606830 PE=3 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 1.7e-101
Identity = 191/362 (52.76%), Postives = 253/362 (69.89%), Query Frame = 0

Query: 17  LCASIIYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLD 76
           L   +++  FKLF+KL W P RI+RFMRSQGIQGP   FI GN   +   +M++M T +D
Sbjct: 13  LSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVYIKRMQAMATPMD 72

Query: 77  ITDHNILPRVHP--------------HWNGNMAQLIVSDPETIKEILHNQERAIFKMTLN 136
           ++ HNIL RV P               W+G  AQL+++DPE IKE+LH+++++  K  L 
Sbjct: 73  LS-HNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHDRQKSFPKAKLK 132

Query: 137 EDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK 196
               RIFGNG  T+EG++WA  RRIA++AFHG+ LK M PTM+ C E M+E WK   EGK
Sbjct: 133 GHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNY-EGK 192

Query: 197 EVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFKS 256
           E+D F+EFKV T D+I+HTAFGSSY +GK IF  L +L  +  +N +K  +P ISK+ KS
Sbjct: 193 ELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIRLPIISKILKS 252

Query: 257 KDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVE 316
           KD  EG+ L ++++   + IIK REEK+ +GEA +YGNDFL LL+KAKN+ +  Q IS+E
Sbjct: 253 KDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKNDPETSQCISME 312

Query: 317 DVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLK 365
           D+VDECK  YF+G ETT VLL+WT  LLA H  WQE+AR EVLD+FG++NPTLEG+ KLK
Sbjct: 313 DIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNNPTLEGLPKLK 372

BLAST of Spg025773 vs. ExPASy TrEMBL
Match: A0A1S3BF46 (cytochrome P450 CYP749A22-like OS=Cucumis melo OX=3656 GN=LOC103489195 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 5.0e-101
Identity = 188/367 (51.23%), Postives = 255/367 (69.48%), Query Frame = 0

Query: 17  LCASIIYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLD 76
           L   ++++ FKLF+KL W P RI+RFMRSQGIQGP   FI GN   +   +M++M T +D
Sbjct: 13  LSGCLLFVVFKLFVKLWWTPMRIQRFMRSQGIQGPSYKFIQGNTRDVYMKRMQAMATPMD 72

Query: 77  ITDHNILPRVHP--------------HWNGNMAQLIVSDPETIKEILHNQERAIFKMTLN 136
           ++ HNILPRV P               W G  AQL+++DPE IKE+LH+++++  K  L 
Sbjct: 73  LS-HNILPRVMPSVHSWLNLYGRNYLQWAGVDAQLMITDPEMIKEVLHDRQKSFPKAKLK 132

Query: 137 EDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK 196
               RIFGNG  T+EG++W   RRIA++AFHG+ LK M PTM+ C E M+E WK   EGK
Sbjct: 133 GHIDRIFGNGLATAEGQRWVNSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNY-EGK 192

Query: 197 EVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFKS 256
           E+D F+EFKV T D+I+HTAFGSSY +GK IF  L +L  +  +N +K  +P ISK+ KS
Sbjct: 193 ELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLQELCDLSIRNGYKIKLPIISKILKS 252

Query: 257 KDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVE 316
           KD  EG+ L ++++   + IIK REEK+ + EA +YGNDFL LL+KAKN+ +  Q IS+E
Sbjct: 253 KDDYEGERLEKRLKDCFMEIIKEREEKLRNDEASDYGNDFLGLLIKAKNDPETSQSISME 312

Query: 317 DVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLK 370
           D+VDECK  YF+G ETT VLL+WT  LLA H  WQE+AR EV D+FG+++PTLEG+ KLK
Sbjct: 313 DIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVFDVFGHNHPTLEGLPKLK 372

BLAST of Spg025773 vs. ExPASy TrEMBL
Match: A0A5D3CAL6 (Cytochrome P450 CYP749A22-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00230 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 1.4e-98
Identity = 184/350 (52.57%), Postives = 244/350 (69.71%), Query Frame = 0

Query: 27  KLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLDITDHNILPRV 86
           +LF+KL W P RI+RFMRSQGIQGP   FI GN   +   +M++M T +D++ HNILPRV
Sbjct: 427 QLFVKLWWTPMRIQRFMRSQGIQGPSYKFIQGNTRDVYMKRMQAMATPMDLS-HNILPRV 486

Query: 87  HP--------------HWNGNMAQLIVSDPETIKEILHNQERAIFKMTLNEDSRRIFGNG 146
            P               W G  AQL+++DPE IKE+LH+++++  K  L     RIFGNG
Sbjct: 487 MPSVHSWLNLYGRNYLQWAGVDAQLMITDPEMIKEVLHDRQKSFPKAKLKGHIDRIFGNG 546

Query: 147 QLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDLFEEFKV 206
             T+EG++W   RRIA++AFHG+ LK M PTM+ C E M+E WK   EGKE+D F+EFKV
Sbjct: 547 LATAEGQRWVNSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNY-EGKELDAFKEFKV 606

Query: 207 LTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFKSKDAREGDELA 266
            T D+I+HTAFGSSY +GK IF  L +L  +  +N +K  +P ISK+ KSKD  EG+ L 
Sbjct: 607 FTLDVISHTAFGSSYQQGKKIFHMLQELCDLSIRNGYKIKLPIISKILKSKDDYEGERLE 666

Query: 267 RKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVEDVVDECKAMY 326
           ++++   + IIK REEK+ + EA +YGNDFL LL+KAKN+ +  Q IS+ED+VDECK  Y
Sbjct: 667 KRLKDCFMEIIKEREEKLRNDEASDYGNDFLGLLIKAKNDPETSQSISMEDIVDECKTFY 726

Query: 327 FSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLK 363
           F+G ETT VLL+WT  LLA H  WQE+AR EV D+FG+++PTLEG+ KLK
Sbjct: 727 FAGHETTNVLLAWTMLLLALHKEWQEKARNEVFDVFGHNHPTLEGLPKLK 774

BLAST of Spg025773 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 221.5 bits (563), Expect = 1.1e-57
Identity = 136/381 (35.70%), Postives = 209/381 (54.86%), Query Frame = 0

Query: 11  VLLLGLLCASII--YLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKM 70
           V+ L L+   +I     FK F+ L W PF + R +++QGI GP     YGNL +I KMK 
Sbjct: 4   VIFLALIVLILIIGLRIFKAFMILVWHPFVLTRRLKNQGISGPNYRIFYGNLSEIKKMKR 63

Query: 71  ESMRTSLDITDHNILPRVHPH--------------WNGNMAQLIVSDPETIKEILHNQER 130
           ES  + LD + ++I PR+ PH              WNG   ++ +SDPE  K +L N+  
Sbjct: 64  ESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTMLSNKLG 123

Query: 131 AIFKMTLNEDSRRIFGN-GQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVE 190
              K     ++ ++ G+ G +  EG  W + RRI + AF  + LK M   MV C   M+E
Sbjct: 124 FFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVDCTLKMLE 183

Query: 191 KWKKQPEGKEV-------DLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFK 250
           +W+K+   +E        ++ EEF+ LT+DIIA +AFGSSY+EG  +F +  +L   +  
Sbjct: 184 EWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELKRCYTT 243

Query: 251 NQFKRGIPGISKLFKSKDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALL 310
           +  +  IPG   L    + R   +L RKM +SI  II +R +        +YG+D L +L
Sbjct: 244 SLNQVSIPGTQYLPTPSNIRVW-KLERKMDNSIKRIISSRLQ-----SKSDYGDDLLGIL 303

Query: 311 LKAKNNSDQRQRISVEDVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLD 367
           LKA N   + +++S+E+++ EC+  +F G ETT  LL+WT  LL+ H  WQE+ R+E+  
Sbjct: 304 LKAYNTEGKERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKLREEIFK 363

BLAST of Spg025773 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 214.2 bits (544), Expect = 1.8e-55
Identity = 128/353 (36.26%), Postives = 192/353 (54.39%), Query Frame = 0

Query: 32  LCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLDITDHNILPRVHPH-- 91
           L W+P+ + R  + QGI GP    +YGNL +I KMK E+    LD   ++I+PRV PH  
Sbjct: 84  LVWRPWMLSRRFKKQGISGPKYRILYGNLREIRKMKNEAKLMVLDPNSNDIVPRVLPHLQ 143

Query: 92  ------------WNGNMAQLIVSDPETIKEILHNQERAIFKMTLNEDSRRIFGNGQLTSE 151
                       W G   +L +SD E  K+IL N+     K     +  ++ GNG +   
Sbjct: 144 QWKSQYGETFLYWQGTDPRLCISDHELAKQILSNKFVFFSKSKTKPEILKLSGNGLIFVN 203

Query: 152 GEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDLF----EEFKVL 211
           G  W + RRI + AF  + LK M   MV C   M  +WKKQ  G E + F     EFK L
Sbjct: 204 GLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFRMFLEWKKQRNGVETEQFVLISREFKRL 263

Query: 212 TSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFKSKDAREGDELAR 271
           T+DIIA  AFGSSY EG  +F++  +L             PGI  L    + +   +L  
Sbjct: 264 TADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGIQYLPTPSNLQIW-KLDM 323

Query: 272 KMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVEDVVDECKAMYF 331
           K+ SSI  II  R    ++ E+++YGND L ++L A ++++  +++S++++++ECK  +F
Sbjct: 324 KVNSSIKRIIDAR----LTSESKDYGNDLLGIMLTAASSNESEKKMSIDEIIEECKTFFF 383

Query: 332 SGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDN-PTLEGITKLKIVS 366
           +G ETT  LL+W+  LL+ H  WQE+ R+EV +  G D  P  E  +KLK+++
Sbjct: 384 AGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPDAETCSKLKLMN 431

BLAST of Spg025773 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 206.1 bits (523), Expect = 5.0e-53
Identity = 131/381 (34.38%), Postives = 215/381 (56.43%), Query Frame = 0

Query: 11  VLLLGLLCASIIYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMES 70
           VL+L ++ + +I     L   L W+P +I      QGI+GPP  F  GN+ +++ M +++
Sbjct: 13  VLVLSVILSLVIVKGMSL---LWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKA 72

Query: 71  MRTSLDITDHNILPRV---HPHWN-----------GNMAQLIVSDPETIKEILHNQERAI 130
               +  + HNILPRV   + HW            G   +L V+DP+ I+EI    E   
Sbjct: 73  SSHPMPFS-HNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIFSKSE-FY 132

Query: 131 FKMTLNEDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKW- 190
            K   +   +++ G+G L+ +GEKWA  R+I    FH E LK + P ++     MV+KW 
Sbjct: 133 EKNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWS 192

Query: 191 -KKQPEGK-EVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGI 250
            K    G+ EVD++E F++LT D+I+ TAFGSSY +G+ +F    +  ++  +   K  I
Sbjct: 193 DKLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFI 252

Query: 251 PGISKLFKSKDAREGDELARKMQSSIISIIKNREEKVISGEAEN----YGNDFLALLLKA 310
           PG  + F ++   +  +L ++++ S++ +I+ R +  I GE E        D L L+++A
Sbjct: 253 PGY-RFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQA 312

Query: 311 KNNSDQRQRISVEDVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFG 370
           KN       ++V+D+V+ECK+ +F+G++TT  LL+WT  LL+ H  WQ +AR EVL + G
Sbjct: 313 KN-------VTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCG 372

BLAST of Spg025773 vs. TAIR 10
Match: AT3G14650.1 (cytochrome P450, family 72, subfamily A, polypeptide 11 )

HSP 1 Score: 202.6 bits (514), Expect = 5.5e-52
Identity = 125/354 (35.31%), Postives = 191/354 (53.95%), Query Frame = 0

Query: 34  WKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLDITDHNILPRVHPH---- 93
           +KP  +  ++R QG+ G P + + G+L +   M+ E+    +++TD +I PR+ P+    
Sbjct: 30  FKPKMLESYLRRQGLAGTPYTPLVGDLKKNFSMRAEARSKPINLTD-DITPRIVPYPLQM 89

Query: 94  ----------WNGNMAQLIVSDPETIKEILHNQERAIFKMTLNEDSRRIFGNGQLTSEGE 153
                     W G +  + + DPE I E+L+      F+        R+   G L+ +G+
Sbjct: 90  LKTHGRTFFTWFGAIPTITIMDPEQITEVLNKVYD--FQKAHTFPLGRLIATGVLSYDGD 149

Query: 154 KWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK----EVDLFEEFKVLTS 213
           KWAK RRI + AFH E +K M P        +V KW K    K    EVD++     +T+
Sbjct: 150 KWAKHRRIINPAFHLEKIKNMVPAFHQSCSEIVCKWDKLVSDKESSCEVDVWPGLVSMTA 209

Query: 214 DIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFKSKDAREGDELARKM 273
           D+I+ TAFGSS +EG+ IFE   +LA +  +   K  IPG S L  +K  R     AR++
Sbjct: 210 DVISRTAFGSSCVEGQRIFELQAELAQLIIQTVRKAFIPGYSYL-PTKGNRRMKAKAREI 269

Query: 274 QSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVEDVVDECKAMYFSG 333
           Q  +  I+  R     +GEA N  +D L +LL++     +   +S ED+++ECK  YF G
Sbjct: 270 QVILRGIVNKRLRAREAGEAPN--DDLLGILLESNLGQTKGNGMSTEDLMEECKLFYFVG 329

Query: 334 QETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLKIVSMSLH 370
           QETT VLL WT  LL+ H  WQ  AR+EV  +FG+  P  EG+ +LK+++M L+
Sbjct: 330 QETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDAEGLNQLKVMTMILY 377

BLAST of Spg025773 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 199.5 bits (506), Expect = 4.7e-51
Identity = 124/353 (35.13%), Postives = 189/353 (53.54%), Query Frame = 0

Query: 36  PFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLDITDHNILPRVHPH------ 95
           P RI++FM  QGI GP    + GN+  I KM   S         HNI+PR+ PH      
Sbjct: 33  PRRIKKFMERQGITGPKPRLLTGNIIDISKMLSHSASNDCSSIHHNIVPRLLPHYVSWSK 92

Query: 96  --------WNGNMAQLIVSDPETIKEILHNQERAIFKMTLNEDSRRIF-GNGQLTSEGEK 155
                   WNG   +L +++ E IKE+L        K  L +   + F G G L + GE 
Sbjct: 93  QYGKRFIMWNGTEPRLCLTETEMIKELLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEA 152

Query: 156 WAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDLFEEFKVLTSDIIAH 215
           W   R +A  AF  + LK     MV C + M E+ +K+  G+EV++ EE + LT+DII+ 
Sbjct: 153 WHHQRHMAAPAFTRDRLKGYAKHMVECTKMMAERLRKE-VGEEVEIGEEMRRLTADIISR 212

Query: 216 TAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFKSKDAREGDELARKMQSSII 275
           T FGSS  +GK +F  LT L  +  +       PG S+   SK  RE   L  +++  ++
Sbjct: 213 TEFGSSCDKGKELFSLLTVLQRLCAQATRHLCFPG-SRFLPSKYNREIKSLKTEVERLLM 272

Query: 276 SIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVEDVVDECKAMYFSGQETTG 335
            II +R++ V  G + +YG+D L LLL   +++  +  ++V+ ++DECK  +F+G ETT 
Sbjct: 273 EIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSN--KNNLNVQMIMDECKTFFFTGHETTS 332

Query: 336 VLLSWTAFLLATHVHWQEEARKEVLDMFGNDN-PTLEGITKL----KIVSMSL 369
           +LL+WT  LLA +  WQ+  R EV  + G D  P++E ++ L    K+++ SL
Sbjct: 333 LLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESL 381

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149008.13.2e-15576.08cytochrome P450 CYP749A22-like [Momordica charantia][more]
XP_022149052.12.4e-10252.07cytochrome P450 CYP749A22-like [Momordica charantia] >XP_022149053.1 cytochrome ... [more]
XP_038892924.14.2e-10252.86cytochrome P450 CYP749A22-like isoform X1 [Benincasa hispida][more]
XP_004135430.29.3e-10252.04cytochrome P450 CYP749A22 isoform X1 [Cucumis sativus] >KAE8648770.1 hypothetica... [more]
XP_008446466.11.0e-10051.23PREDICTED: cytochrome P450 CYP749A22-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
H2DH175.9e-9144.35Cytochrome P450 CYP749A22 OS=Panax ginseng OX=4054 PE=2 SV=1[more]
Q9ASR31.6e-5635.70Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1[more]
F4IK452.6e-5436.26Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1[more]
Q2MJ211.6e-5135.18Cytochrome P450 716A67 OS=Medicago truncatula OX=3880 GN=CYP72A67 PE=2 SV=1[more]
Q2MJ193.5e-5133.24Cytochrome P450 72A68 OS=Medicago truncatula OX=3880 GN=CYP72A68 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D5N21.6e-15576.08cytochrome P450 CYP749A22-like OS=Momordica charantia OX=3673 GN=LOC111017532 PE... [more]
A0A6J1D6R81.2e-10252.07cytochrome P450 CYP749A22-like OS=Momordica charantia OX=3673 GN=LOC111017564 PE... [more]
A0A0A0KQY31.7e-10152.76Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G606830 PE=3 SV=1[more]
A0A1S3BF465.0e-10151.23cytochrome P450 CYP749A22-like OS=Cucumis melo OX=3656 GN=LOC103489195 PE=3 SV=1[more]
A0A5D3CAL61.4e-9852.57Cytochrome P450 CYP749A22-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
Match NameE-valueIdentityDescription
AT2G46960.21.1e-5735.70cytochrome P450, family 709, subfamily B, polypeptide 1 [more]
AT2G46950.11.8e-5536.26cytochrome P450, family 709, subfamily B, polypeptide 2 [more]
AT2G26710.15.0e-5334.38Cytochrome P450 superfamily protein [more]
AT3G14650.15.5e-5235.31cytochrome P450, family 72, subfamily A, polypeptide 11 [more]
AT1G67110.14.7e-5135.13cytochrome P450, family 735, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PFAMPF00067p450coord: 50..369
e-value: 9.6E-34
score: 116.9
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 36..369
e-value: 6.8E-60
score: 204.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 46..366
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 12..367
NoneNo IPR availablePANTHERPTHR24282:SF187CYTOCHROMEcoord: 12..367

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg025773.1Spg025773.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen