Spg024845 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg024845
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	enoyl-CoA hydratase/isomerase A
Location	scaffold12: 11806709 .. 11808148 (+)
RNA-Seq Expression	Spg024845
Synteny	Spg024845

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCGTTCTTCGCCGGAGAAGCTCATCCTCGTCAACCGAGAACCAAATGGGATCGCATTCGTCACTATCAACCGGCCCAAGTCGTTGAACTCGTTGACGAGGCCGATGATGACGGATTTGGCTCAGGCGTTCAAGAGCTTGGACCGCGACGAGTCAGTGCGAGTCATCGTGCTCTCTGGTTCAGGACGAGCGTTTTGTTCCGGTGTAGACCTTACTGCGGCGGAGGACGTCTTCAAGGGAGATGTGAAGGATGTTGAATCCGATCCGGTTGTGCAAATGGAACGCTGCCGGAAGCCGATCATCGGAGCGATTGCAGGTTTCGCCATCACCGCTGGGTTTGAGATCGCGTTGGCTTGTGACATGCTCATCGCCGCCAAAGGAGCTAAGTTTATCGATACTCATGCTAGGTTTGTCATTGTACTAAGTTCACCCAATTTTTTGTGCGCTAGGACGATTCTTATTATTAGCTAGGGTTATGGCGATTACGATTCAGAATCAAGAATGTGTGATTTTCTGAAATCTATTGGCAACGCTGTTCTAGTGGAATTTCTGATTTCTGGACAAATTTGTGGCAGATTTGGGATATTTCCTTCATGGGGATTATCTCAGAAACTCCCAAGGATCATTGGAGTTAACAAAGCTCGTGAAGTATCCCTAACGGCGATTCCTCTAACTGCAGAGGAAGCAGAGAAGAGGGGTTTGGTGAATCATGTGGTCGAAGGAGGTGAATTGTTGAAGAAAGCTCGAGAAGTTGCAGAGGCCATTTTAAAGAACAATCAGGACTTGGTGTTGAGATACAAGTCAGTCATTAACGATGGCCTGAAGCTGGAGCTTGGCCATGCTCTTGCGCTGGAAAAGGTAATTTCCTTGATAACTTCACATACCGAACCTGTTGATCATCGAAACTAGAAATTCTCATTGTGATTTCTATTATACTCGAGTTTATCCCCAAATCTGTATTGATAATTGACTTGGGATAGTTTTTTAGTACTTTTTGGATGACAGAACTTGTGTATTAGCATTCTGCAACTGACTGTTTGTTTTGTTGATTCAGGAGAGAGCTCATGCGTATTACAATGGAATGACCAAAGAGCAATTCCAGAAAATGCAGGAATTCATAGCTGGTCGGAGCTCCAAGAAACCTTCCAAGTTATAGTCTAAACGACGTAGTTTCCTGTCTCTGTTTCTGTACGGTAACAATAATATGGAAAAACTTGCCTCCTCACTGGTTAGTTGATTCCTAGTCCCTACTTTTATTTATACATGTAATTGACTTGAGTGGATAAGAAGTATCTGTGCCGTTAATATATTTATTGAATATAAGTTAACTTGTAGAAATAGATTAGGTAGAAAATAAGGAGGGGATGGGAAGGCTAGTTTAATAAGTGTAGTAATATTTGGTGGCTATTTTGAGAAGAATGCAGTCCGGTTTCTGGG

mRNA sequence

ATGGATCGTTCTTCGCCGGAGAAGCTCATCCTCGTCAACCGAGAACCAAATGGGATCGCATTCGTCACTATCAACCGGCCCAAGTCGTTGAACTCGTTGACGAGGCCGATGATGACGGATTTGGCTCAGGCGTTCAAGAGCTTGGACCGCGACGAGTCAGTGCGAGTCATCGTGCTCTCTGGTTCAGGACGAGCGTTTTGTTCCGGTGTAGACCTTACTGCGGCGGAGGACGTCTTCAAGGGAGATGTGAAGGATGTTGAATCCGATCCGGTTGTGCAAATGGAACGCTGCCGGAAGCCGATCATCGGAGCGATTGCAGGTTTCGCCATCACCGCTGGGTTTGAGATCGCGTTGGCTTGTGACATGCTCATCGCCGCCAAAGGAGCTAAGTTTATCGATACTCATGCTAGATTTGGGATATTTCCTTCATGGGGATTATCTCAGAAACTCCCAAGGATCATTGGAGTTAACAAAGCTCGTGAAGTATCCCTAACGGCGATTCCTCTAACTGCAGAGGAAGCAGAGAAGAGGGGTTTGGTGAATCATGTGGTCGAAGGAGGTGAATTGTTGAAGAAAGCTCGAGAAGTTGCAGAGGCCATTTTAAAGAACAATCAGGACTTGGTGTTGAGATACAAGTCAGTCATTAACGATGGCCTGAAGCTGGAGCTTGGCCATGCTCTTGCGCTGGAAAAGGAGAGAGCTCATGCGTATTACAATGGAATGACCAAAGAGCAATTCCAGAAAATGCAGGAATTCATAGCTGGTCGGAGCTCCAAGAAACCTTCCAAGTTATAG

Coding sequence (CDS)

ATGGATCGTTCTTCGCCGGAGAAGCTCATCCTCGTCAACCGAGAACCAAATGGGATCGCATTCGTCACTATCAACCGGCCCAAGTCGTTGAACTCGTTGACGAGGCCGATGATGACGGATTTGGCTCAGGCGTTCAAGAGCTTGGACCGCGACGAGTCAGTGCGAGTCATCGTGCTCTCTGGTTCAGGACGAGCGTTTTGTTCCGGTGTAGACCTTACTGCGGCGGAGGACGTCTTCAAGGGAGATGTGAAGGATGTTGAATCCGATCCGGTTGTGCAAATGGAACGCTGCCGGAAGCCGATCATCGGAGCGATTGCAGGTTTCGCCATCACCGCTGGGTTTGAGATCGCGTTGGCTTGTGACATGCTCATCGCCGCCAAAGGAGCTAAGTTTATCGATACTCATGCTAGATTTGGGATATTTCCTTCATGGGGATTATCTCAGAAACTCCCAAGGATCATTGGAGTTAACAAAGCTCGTGAAGTATCCCTAACGGCGATTCCTCTAACTGCAGAGGAAGCAGAGAAGAGGGGTTTGGTGAATCATGTGGTCGAAGGAGGTGAATTGTTGAAGAAAGCTCGAGAAGTTGCAGAGGCCATTTTAAAGAACAATCAGGACTTGGTGTTGAGATACAAGTCAGTCATTAACGATGGCCTGAAGCTGGAGCTTGGCCATGCTCTTGCGCTGGAAAAGGAGAGAGCTCATGCGTATTACAATGGAATGACCAAAGAGCAATTCCAGAAAATGCAGGAATTCATAGCTGGTCGGAGCTCCAAGAAACCTTCCAAGTTATAG

Protein sequence

MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLSGSGRAFCSGVDLTAAEDVFKGDVKDVESDPVVQMERCRKPIIGAIAGFAITAGFEIALACDMLIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLVNHVVEGGELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLELGHALALEKERAHAYYNGMTKEQFQKMQEFIAGRSSKKPSKL

Homology

[Close]

BLAST of Spg024845 vs. NCBI nr
Match: XP_022959404.1 (probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 492.3 bits (1266), Expect = 2.7e-135
Identity = 249/264 (94.32%), Postives = 261/264 (98.86%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLS 60
           MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTR MM DLAQAFK+LDRDESVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVVQMERCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVE+DPVVQMERCRKPIIGA+AGFA+TAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAVTAGFEIALAC 120

Query: 121 DMLIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLV 180
           D+LIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTA+PLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLELGHALALEKERAHAYYNG 240
           NHVV+GGELLKK RE+AEAILKNNQDLVLRYKSVINDGLKL+LGHALALEKERAHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKKPSKL 265
           MTKEQF+KMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Spg024845 vs. NCBI nr
Match: KAG6575117.1 (putative enoyl-CoA hydratase 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 490.3 bits (1261), Expect = 1.0e-134
Identity = 249/264 (94.32%), Postives = 260/264 (98.48%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLS 60
           MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTR MM DLAQAFK+LDRDESVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVVQMERCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVE+DPVVQMERCRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DMLIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLV 180
           D+LIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTA+PLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLELGHALALEKERAHAYYNG 240
           NHVV+GGELLKK RE+AEAILKNNQDLVLRYKSVINDGLKL+LGHALALEKERAHAYY G
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYTG 240

Query: 241 MTKEQFQKMQEFIAGRSSKKPSKL 265
           MTKEQF+KMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Spg024845 vs. NCBI nr
Match: XP_023548460.1 (probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 490.3 bits (1261), Expect = 1.0e-134
Identity = 249/264 (94.32%), Postives = 260/264 (98.48%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLS 60
           MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTR MM DLAQAFKSLDRDESVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVVQMERCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVE+DPVVQMERCRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DMLIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLV 180
           D+LIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGV+KAREVSLTA+PLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLELGHALALEKERAHAYYNG 240
           NHVV+GGELLKK RE+AEAILKNNQDLVLRYKSVINDGLKL+LGHALALEKER HAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKKPSKL 265
           MTKEQF+KMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Spg024845 vs. NCBI nr
Match: XP_023006283.1 (probable enoyl-CoA hydratase 1, peroxisomal isoform X1 [Cucurbita maxima])

HSP 1 Score: 489.6 bits (1259), Expect = 1.7e-134
Identity = 249/264 (94.32%), Postives = 260/264 (98.48%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLS 60
           MDRSS EKLILVNREPNGIAFVTINRPKSLNSLTR MM DLAQAFKSLDRDESVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVVQMERCRKPIIGAIAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVE+DPVVQMERCRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DMLIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLV 180
           D+LIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTA+PLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLELGHALALEKERAHAYYNG 240
           NHVV+GGELLKK RE+AEAILKNNQDLVLRYKSVINDGLKL+LGHALALEKERAHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKKPSKL 265
           MTKEQF+KMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Spg024845 vs. NCBI nr
Match: XP_023006284.1 (probable enoyl-CoA hydratase 1, peroxisomal isoform X2 [Cucurbita maxima])

HSP 1 Score: 486.5 bits (1251), Expect = 1.5e-133
Identity = 248/264 (93.94%), Postives = 259/264 (98.11%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLS 60
           MDRSS EKLILVNREPNGIAFVTINRPKSLNSLTR MM DLAQAFKSLDRDESVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVVQMERCRKPIIGAIAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVE+DPVVQMERCRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DMLIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLV 180
           D+LIAAKGAKFIDTHARF IFPSWGLSQKLPRIIGVNKAREVSLTA+PLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLELGHALALEKERAHAYYNG 240
           NHVV+GGELLKK RE+AEAILKNNQDLVLRYKSVINDGLKL+LGHALALEKERAHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKKPSKL 265
           MTKEQF+KMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Spg024845 vs. ExPASy Swiss-Prot
Match: Q6NL24 (Probable enoyl-CoA hydratase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECHIA PE=1 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 8.7e-113
Identity = 205/262 (78.24%), Postives = 232/262 (88.55%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLS 60
           MD++  E LI V +E  GIA +TINRPKSLNSLTR MM DLA+AFK +D DESV+V++ +
Sbjct: 1   MDQTVSENLIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFT 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVVQMERCRKPIIGAIAGFAITAGFEIALAC 120
           GSGR+FCSGVDLTAAE VFKGDVKD E+DPVVQMER RKPIIGAI GFAITAGFE+ALAC
Sbjct: 61  GSGRSFCSGVDLTAAESVFKGDVKDPETDPVVQMERLRKPIIGAINGFAITAGFELALAC 120

Query: 121 DMLIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLV 180
           D+L+A++GAKF+DTHARFGIFPSWGLSQKL RIIG NKAREVSLT++PLTA+ A K G V
Sbjct: 121 DILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLELGHALALEKERAHAYYNG 240
           NHVVE GE LKKARE+AEAI+KN Q +VLR KSVINDGLKL+LGHAL LEKERAHAYY+G
Sbjct: 181 NHVVEEGEALKKAREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHALTLEKERAHAYYSG 240

Query: 241 MTKEQFQKMQEFIAGRSSKKPS 263
           MTKEQF+KMQEFIAGR SKKPS
Sbjct: 241 MTKEQFRKMQEFIAGRGSKKPS 262

[Close]

BLAST of Spg024845 vs. ExPASy Swiss-Prot
Match: Q1ZXF1 (Probable enoyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=echs1 PE=3 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.5e-32
Identity = 88/242 (36.36%), Postives = 136/242 (56.20%), Query Frame = 0


Query: 19  IAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLSGSGRAFCSGVDLTAAEDV 78
           +  VT+NRPKSLN+L+  +++++  A K    D+ V  I+++GS +AF +G D+   E V
Sbjct: 34  VGLVTLNRPKSLNALSDGLISEINSAVKLFQEDKDVGSIIITGSEKAFAAGADIKEMEKV 93

Query: 79  FKGDVKDVESDPVVQ---MERCRKPIIGAIAGFAITAGFEIALACDMLIAAKGAKFIDTH 138
              D     +D + Q   + + RKPII A+ G+A+  G E+A+ CD++IA + A F    
Sbjct: 94  TLPDA--YNNDLLAQWHDLTKIRKPIIAAVNGYALGGGCELAMMCDIIIAGEKAVFGQPE 153

Query: 139 ARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLVNHVVEGGELLKKARE 198
            + G  P  G +Q+L R IG +KA E+ LT   LTA EAEK GLV+ VV   ELL +A +
Sbjct: 154 IKLGTIPGCGGTQRLIRAIGKSKAMELVLTGNNLTAVEAEKAGLVSKVVPVEELLTEATK 213

Query: 199 VAEAILKNNQDLVLRYKSVINDGLKLELGHALALEKERAHAYYNGMTKEQFQKMQEFIAG 258
           +AE I   +Q  V   K  +N   +L L   +  E+   H+ +   T +Q + M  F+  
Sbjct: 214 MAEKIASYSQLTVAMAKEAVNASYELTLQEGIRFERRMFHSTFG--THDQKEGMNAFVEK 271

[Close]

BLAST of Spg024845 vs. ExPASy Swiss-Prot
Match: P52046 (Short-chain-enoyl-CoA hydratase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=crt PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 9.8e-32
Identity = 83/242 (34.30%), Postives = 140/242 (57.85%), Query Frame = 0


Query: 19  IAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLSGSG-RAFCSGVDLTAAED 78
           +A VTINRPK+LN+L    + ++      ++ D  V  ++L+G+G ++F +G D++  ++
Sbjct: 14  VAVVTINRPKALNALNSDTLKEMDYVIGEIENDSEVLAVILTGAGEKSFVAGADISEMKE 73

Query: 79  V--FKGDVKDVESDPVV-QMERCRKPIIGAIAGFAITAGFEIALACDMLIAAKGAKFIDT 138
           +   +G    +  + V  ++E   KP+I A+ GFA+  G EIA++CD+ IA+  A+F   
Sbjct: 74  MNTIEGRKFGILGNKVFRRLELLEKPVIAAVNGFALGGGCEIAMSCDIRIASSNARFGQP 133

Query: 139 HARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLVNHVVEGGELLKKAR 198
               GI P +G +Q+L R++G+  A+++  TA  + A+EA + GLVN VVE  EL+  A+
Sbjct: 134 EVGLGITPGFGGTQRLSRLVGMGMAKQLIFTAQNIKADEALRIGLVNKVVEPSELMNTAK 193

Query: 199 EVAEAILKNNQDLVLRYKSVINDGLKLELGHALALEKERAHAYYNGMTKEQFQKMQEFIA 257
           E+A  I+ N    V   K  IN G++ ++  ALA E E     ++  T++Q   M  FI 
Sbjct: 194 EIANKIVSNAPVAVKLSKQAINRGMQCDIDTALAFESEAFGECFS--TEDQKDAMTAFIE 253

[Close]

BLAST of Spg024845 vs. ExPASy Swiss-Prot
Match: P64017 (Probable enoyl-CoA hydratase echA8 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=echA8 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.7e-31
Identity = 84/249 (33.73%), Postives = 132/249 (53.01%), Query Frame = 0


Query: 10  ILVNREPNGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLSGSGRAFCSG 69
           ILV R+   +  +T+NRP++LN+L   +M ++  A   LD D  +  I+++GS +AF +G
Sbjct: 6   ILVERDQR-VGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAG 65

Query: 70  VDLTAAEDVFKGDVKDVESDPV-VQMERCRKPIIGAIAGFAITAGFEIALACDMLIAAKG 129
            D+    D+   D    +      ++   R P I A+AG+A+  G E+A+ CD+LIAA  
Sbjct: 66  ADIKEMADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADT 125

Query: 130 AKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLVNHVVEGGE 189
           AKF     + G+ P  G SQ+L R IG  KA ++ LT   + A EAE+ GLV+ VV   +
Sbjct: 126 AKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADD 185

Query: 190 LLKKAREVAEAILKNNQDLVLRYKSVINDGLKLELGHALALEKERAHAYYNGMTKEQFQK 249
           LL +AR  A  I + +       K  +N   +  L   L  E+   H+ +   T++Q + 
Sbjct: 186 LLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAF--ATEDQSEG 245

Query: 250 MQEFIAGRS 258
           M  FI  R+
Sbjct: 246 MAAFIEKRA 251

[Close]

BLAST of Spg024845 vs. ExPASy Swiss-Prot
Match: P9WNN8 (Probable enoyl-CoA hydratase echA8 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=echA8 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.7e-31
Identity = 84/249 (33.73%), Postives = 132/249 (53.01%), Query Frame = 0


Query: 10  ILVNREPNGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLSGSGRAFCSG 69
           ILV R+   +  +T+NRP++LN+L   +M ++  A   LD D  +  I+++GS +AF +G
Sbjct: 6   ILVERDQR-VGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAG 65

Query: 70  VDLTAAEDVFKGDVKDVESDPV-VQMERCRKPIIGAIAGFAITAGFEIALACDMLIAAKG 129
            D+    D+   D    +      ++   R P I A+AG+A+  G E+A+ CD+LIAA  
Sbjct: 66  ADIKEMADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADT 125

Query: 130 AKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLVNHVVEGGE 189
           AKF     + G+ P  G SQ+L R IG  KA ++ LT   + A EAE+ GLV+ VV   +
Sbjct: 126 AKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADD 185

Query: 190 LLKKAREVAEAILKNNQDLVLRYKSVINDGLKLELGHALALEKERAHAYYNGMTKEQFQK 249
           LL +AR  A  I + +       K  +N   +  L   L  E+   H+ +   T++Q + 
Sbjct: 186 LLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAF--ATEDQSEG 245

Query: 250 MQEFIAGRS 258
           M  FI  R+
Sbjct: 246 MAAFIEKRA 251

[Close]

BLAST of Spg024845 vs. ExPASy TrEMBL
Match: A0A6J1H7Z0 (probable enoyl-CoA hydratase 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111460389 PE=4 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.3e-135
Identity = 249/264 (94.32%), Postives = 261/264 (98.86%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLS 60
           MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTR MM DLAQAFK+LDRDESVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVVQMERCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVE+DPVVQMERCRKPIIGA+AGFA+TAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAVTAGFEIALAC 120

Query: 121 DMLIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLV 180
           D+LIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTA+PLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLELGHALALEKERAHAYYNG 240
           NHVV+GGELLKK RE+AEAILKNNQDLVLRYKSVINDGLKL+LGHALALEKERAHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKKPSKL 265
           MTKEQF+KMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Spg024845 vs. ExPASy TrEMBL
Match: A0A6J1L4H0 (probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499066 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 8.3e-135
Identity = 249/264 (94.32%), Postives = 260/264 (98.48%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLS 60
           MDRSS EKLILVNREPNGIAFVTINRPKSLNSLTR MM DLAQAFKSLDRDESVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVVQMERCRKPIIGAIAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVE+DPVVQMERCRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DMLIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLV 180
           D+LIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTA+PLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLELGHALALEKERAHAYYNG 240
           NHVV+GGELLKK RE+AEAILKNNQDLVLRYKSVINDGLKL+LGHALALEKERAHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKKPSKL 265
           MTKEQF+KMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Spg024845 vs. ExPASy TrEMBL
Match: A0A6J1L1R6 (probable enoyl-CoA hydratase 1, peroxisomal isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499066 PE=4 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 7.1e-134
Identity = 248/264 (93.94%), Postives = 259/264 (98.11%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLS 60
           MDRSS EKLILVNREPNGIAFVTINRPKSLNSLTR MM DLAQAFKSLDRDESVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVVQMERCRKPIIGAIAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVE+DPVVQMERCRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DMLIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLV 180
           D+LIAAKGAKFIDTHARF IFPSWGLSQKLPRIIGVNKAREVSLTA+PLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLELGHALALEKERAHAYYNG 240
           NHVV+GGELLKK RE+AEAILKNNQDLVLRYKSVINDGLKL+LGHALALEKERAHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKKPSKL 265
           MTKEQF+KMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Spg024845 vs. ExPASy TrEMBL
Match: A0A6J1CAG2 (probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009856 PE=4 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 6.6e-132
Identity = 245/264 (92.80%), Postives = 258/264 (97.73%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLS 60
           MD SSPEKLILV+REPNGIAFVTINRPKSLNSLTRPM+ DLA A KSLDRD+SVRVIVLS
Sbjct: 1   MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVVQMERCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDL+AAE+VFKGDVKDVESDPV+QMERCRKPIIGAIAGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DMLIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLV 180
           D+LIAAKGAKFIDTHARFGIFPSWGLSQKL RIIGVNKAREVSLTA+PLTAEEAE+RGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLELGHALALEKERAHAYYNG 240
           NHVVEGGELLKKAREVAEAILKNNQD+VLRYKSVINDGL+L+LGHALALEKER HAYYNG
Sbjct: 181 NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKKPSKL 265
           MTKEQF+KMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Spg024845 vs. ExPASy TrEMBL
Match: A0A1S3C8X4 (probable enoyl-CoA hydratase 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103497788 PE=4 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.1e-131
Identity = 247/264 (93.56%), Postives = 255/264 (96.59%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLS 60
           MDRSSPE LILVNRE NGIAF+TINRPKSLNSLTRPMM DLA+AFKSLDRD+SVRVI+LS
Sbjct: 1   MDRSSPENLILVNRESNGIAFITINRPKSLNSLTRPMMIDLARAFKSLDRDDSVRVIILS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVVQMERCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVESDPV QME CRKPIIGAIAGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DMLIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLV 180
           D+LIAAKGAKFIDTHARFGIFPSWGLSQKL RIIGVNKAREVSLTA+PLTAEEAEKRGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLELGHALALEKERAHAYYNG 240
           N VVEG ELLKKAREVAEAILKNNQDLVLRYKSVINDGLKL+LG ALALEKERAHAYYNG
Sbjct: 181 NQVVEGSELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKKPSKL 265
           MTKEQFQKMQEFIAGRSSK+PSKL
Sbjct: 241 MTKEQFQKMQEFIAGRSSKRPSKL 264

[Close]

BLAST of Spg024845 vs. TAIR 10
Match: AT4G16210.1 (enoyl-CoA hydratase/isomerase A )

HSP 1 Score: 407.9 bits (1047), Expect = 6.1e-114
Identity = 205/262 (78.24%), Postives = 232/262 (88.55%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLS 60
           MD++  E LI V +E  GIA +TINRPKSLNSLTR MM DLA+AFK +D DESV+V++ +
Sbjct: 1   MDQTVSENLIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFT 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVVQMERCRKPIIGAIAGFAITAGFEIALAC 120
           GSGR+FCSGVDLTAAE VFKGDVKD E+DPVVQMER RKPIIGAI GFAITAGFE+ALAC
Sbjct: 61  GSGRSFCSGVDLTAAESVFKGDVKDPETDPVVQMERLRKPIIGAINGFAITAGFELALAC 120

Query: 121 DMLIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLV 180
           D+L+A++GAKF+DTHARFGIFPSWGLSQKL RIIG NKAREVSLT++PLTA+ A K G V
Sbjct: 121 DILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLELGHALALEKERAHAYYNG 240
           NHVVE GE LKKARE+AEAI+KN Q +VLR KSVINDGLKL+LGHAL LEKERAHAYY+G
Sbjct: 181 NHVVEEGEALKKAREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHALTLEKERAHAYYSG 240

Query: 241 MTKEQFQKMQEFIAGRSSKKPS 263
           MTKEQF+KMQEFIAGR SKKPS
Sbjct: 241 MTKEQFRKMQEFIAGRGSKKPS 262

[Close]

BLAST of Spg024845 vs. TAIR 10
Match: AT1G60550.1 (enoyl-CoA hydratase/isomerase D )

HSP 1 Score: 114.4 bits (285), Expect = 1.4e-25
Identity = 78/214 (36.45%), Postives = 112/214 (52.34%), Query Frame = 0


Query: 18  GIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLSGSG-RAFCSGVDLTAAE 77
           GIA +TINRP+  N+     + +L +AF     D SV VI+L+G G +AFCSG D     
Sbjct: 86  GIAKITINRPERRNAFRPQTVKELMRAFNDARDDSSVGVIILTGKGTKAFCSGGDQALRT 145

Query: 78  DVFKGDVKDVES----DPVVQMERCRKPIIGAIAGFAITAGFEIALACDMLIAAKGAKFI 137
                D  DV      D  VQ+ R  KP+I  +AG+A+  G  + + CD+ IAA  A F 
Sbjct: 146 QDGYADPNDVGRLNVLDLQVQIRRLPKPVIAMVAGYAVGGGHILHMVCDLTIAADNAIFG 205

Query: 138 DTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLVNHVVEGGELLKK 197
            T  + G F +   S  + R++G  KARE+       TA EAEK GL+N VV   +L K+
Sbjct: 206 QTGPKVGSFDAGYGSSIMSRLVGPKKAREMWFMTRFYTASEAEKMGLINTVVPLEDLEKE 265

Query: 198 AREVAEAILKNNQDLVLRYKSVINDGLKLELGHA 227
             +    IL+N+   +   K+ +N    ++ GHA
Sbjct: 266 TVKWCREILRNSPTAIRVLKAALN---AVDDGHA 296

[Close]

BLAST of Spg024845 vs. TAIR 10
Match: AT4G29010.1 (Enoyl-CoA hydratase/isomerase family )

HSP 1 Score: 108.6 bits (270), Expect = 7.7e-24
Identity = 62/186 (33.33%), Postives = 109/186 (58.60%), Query Frame = 0


Query: 17  NGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLSGSGRAFCSGVDLTAAE 76
           +G+A +TI+ P  +NSL  P+++ L + F+  ++   V+ IVL G+   F  G D+   +
Sbjct: 14  DGVAVITISNP-PVNSLASPIISGLKEKFRDANQRNDVKAIVLIGNNGRFSGGFDINVFQ 73

Query: 77  DVFK-GDVK---DVESDPVVQ-MERCRKPIIGAIAGFAITAGFEIALACDMLIAAKGAKF 136
            V K GD+    +V  + V   ME  RKP++ A+ G A+  G E+A+AC   +AA  A+ 
Sbjct: 74  QVHKTGDLSLMPEVSVELVCNLMEDSRKPVVAAVEGLALGGGLELAMACHARVAAPKAQL 133

Query: 137 IDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLVNHVVEGGELLK 196
                  G+ P +G +Q+LPR++G+ KA ++ L +  +++EE  K GL++ +V  G++L 
Sbjct: 134 GLPELTLGVIPGFGGTQRLPRLVGLAKATDMILLSKSISSEEGHKLGLIDALVPPGDVLS 193

Query: 197 KAREVA 198
            +R+ A
Sbjct: 194 TSRKWA 198

[Close]

BLAST of Spg024845 vs. TAIR 10
Match: AT4G16800.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 108.6 bits (270), Expect = 7.7e-24
Identity = 72/235 (30.64%), Postives = 121/235 (51.49%), Query Frame = 0


Query: 4   SSPEKLILVNR---EPNGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVL- 63
           +SP + + +NR     +GI  V ++RP + N++ + M+  L  AF+S+ +D S RV+++ 
Sbjct: 37  TSPPEFVKLNRLSGSDSGIIEVNLDRPVTKNAINKEMLKSLQNAFESIHQDNSARVVMIR 96

Query: 64  SGSGRAFCSGVDLTAAEDVFKGDVKDVESDPVVQ---MERCRKPIIGAIAGFAITAGFEI 123
           S     FC+G DL     +   +V    +        +E    P I AI G A+  G E+
Sbjct: 97  SLVPGVFCAGADLKERRTMSPSEVHTYVNSLRYMFSFIEALSIPTIAAIEGAALGGGLEM 156

Query: 124 ALACDMLIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEK 183
           ALACD+ I  + A F        I P  G +Q+L R++G + ++E+  T   + A EA  
Sbjct: 157 ALACDLRICGENAVFGLPETGLAIIPGAGGTQRLSRLVGRSVSKELIFTGRKIDAIEAAN 216

Query: 184 RGLVNHVVEGGELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLELGHALALEK 232
           +GLVN  V  GE  +KA E+A+ I +     +   K  I++G++  +   L +E+
Sbjct: 217 KGLVNICVTAGEAHEKAIEMAQQINEKGPLAIKMAKKAIDEGIETNMASGLEVEE 271

[Close]

BLAST of Spg024845 vs. TAIR 10
Match: AT3G06860.1 (multifunctional protein 2 )

HSP 1 Score: 104.8 bits (260), Expect = 1.1e-22
Identity = 62/187 (33.16%), Postives = 106/187 (56.68%), Query Frame = 0


Query: 17  NGIAFVTINRPKSLNSLTRPMMTDLAQAFKSLDRDESVRVIVLSGSGRAFCSGVDLTAAE 76
           +G+A +T+  P  +NSL+  ++ +L   ++       V+ IV++G+   F  G D++   
Sbjct: 16  DGVAVITLINP-PVNSLSFDVLYNLKSNYEEALSRNDVKAIVITGAKGRFSGGFDISGFG 75

Query: 77  DVFKGDVKD-----VESDPVVQ-MERCRKPIIGAIAGFAITAGFEIALACDMLIAAKGAK 136
           ++ KG+VK+     +  D +   +E  RKP + AI G A+  G E+A+AC   I+A  A+
Sbjct: 76  EMQKGNVKEPKAGYISIDIITDLLEAARKPSVAAIDGLALGGGLELAMACHARISAPAAQ 135

Query: 137 FIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAIPLTAEEAEKRGLVNHVVEGGELL 196
                 + G+ P +G +Q+LPR++G+ KA E+ LT+ P+ AEE    GL++ VV   EL+
Sbjct: 136 LGLPELQLGVIPGFGGTQRLPRLVGLTKALEMILTSKPVKAEEGHSLGLIDAVVPPAELV 195

Query: 197 KKAREVA 198
             AR  A
Sbjct: 196 TTARRWA 201

The following BLAST results are available for this feature:

BLAST of Spg024845 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022959404.1	2.7e-135	94.32	probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita moschata]	[more]
KAG6575117.1	1.0e-134	94.32	putative enoyl-CoA hydratase 1, peroxisomal, partial [Cucurbita argyrosperma sub...	[more]
XP_023548460.1	1.0e-134	94.32	probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita pepo subsp. pepo]	[more]
XP_023006283.1	1.7e-134	94.32	probable enoyl-CoA hydratase 1, peroxisomal isoform X1 [Cucurbita maxima]	[more]
XP_023006284.1	1.5e-133	93.94	probable enoyl-CoA hydratase 1, peroxisomal isoform X2 [Cucurbita maxima]	[more]

BLAST of Spg024845 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NL24	8.7e-113	78.24	Probable enoyl-CoA hydratase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=E...	[more]
Q1ZXF1	1.5e-32	36.36	Probable enoyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum OX=44689...	[more]
P52046	9.8e-32	34.30	Short-chain-enoyl-CoA hydratase OS=Clostridium acetobutylicum (strain ATCC 824 /...	[more]
P64017	1.7e-31	33.73	Probable enoyl-CoA hydratase echA8 OS=Mycobacterium bovis (strain ATCC BAA-935 /...	[more]
P9WNN8	1.7e-31	33.73	Probable enoyl-CoA hydratase echA8 OS=Mycobacterium tuberculosis (strain CDC 155...	[more]

BLAST of Spg024845 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H7Z0	1.3e-135	94.32	probable enoyl-CoA hydratase 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1L4H0	8.3e-135	94.32	probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Cucurbita maxima OX=36...	[more]
A0A6J1L1R6	7.1e-134	93.94	probable enoyl-CoA hydratase 1, peroxisomal isoform X2 OS=Cucurbita maxima OX=36...	[more]
A0A6J1CAG2	6.6e-132	92.80	probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Momordica charantia OX...	[more]
A0A1S3C8X4	1.1e-131	93.56	probable enoyl-CoA hydratase 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103497...	[more]

BLAST of Spg024845 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16210.1	6.1e-114	78.24	enoyl-CoA hydratase/isomerase A	[more]
AT1G60550.1	1.4e-25	36.45	enoyl-CoA hydratase/isomerase D	[more]
AT4G29010.1	7.7e-24	33.33	Enoyl-CoA hydratase/isomerase family	[more]
AT4G16800.1	7.7e-24	30.64	ATP-dependent caseinolytic (Clp) protease/crotonase family protein	[more]
AT3G06860.1	1.1e-22	33.16	multifunctional protein 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001753	Enoyl-CoA hydratase/isomerase	PFAM	PF00378	ECH_1	coord: 15..240 e-value: 5.4E-54 score: 183.1
None	No IPR available	GENE3D	3.90.226.10		coord: 3..239 e-value: 1.5E-68 score: 232.5
None	No IPR available	PANTHER	PTHR43802	ENOYL-COA HYDRATASE	coord: 6..260
None	No IPR available	CDD	cd06558	crotonase-like	coord: 10..200 e-value: 3.75267E-70 score: 212.421
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 8..259

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg024845.1	Spg024845.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009536	plastid
molecular_function	GO:0003824	catalytic activity