Spg022745 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg022745
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCOBRA-like protein
Locationscaffold2: 4284701 .. 4287632 (-)
RNA-Seq ExpressionSpg022745
SyntenySpg022745
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTACAGTCTTGTGGAGTACTTCCCATTCTCTCTCTCTCTCTCCACAGGACACGCCAAGTTTCCTGTGCAGATCTTGAGCCATTAGCAGAACGCCGCCTGCAGCTCGGTCTGCATCGGAAAGATGAGGTCTCGGCGCTTCGCTTCCACTGGATCCGTCGCCGAACTTACCGGCTTTTCCGTCGTGCTTCTCTTTCTACTTTCCAGCTTCAGTTTCACTTCAACAGGTCAGTCAGTGAACTCAAACTTTGTTACTTATTGGTGGTTCCAGACTTGGCTACTGGACGTGGTTTTGTTATCTGTTCATTAATCCATTCTGTTTATCTGATAGTTGGAAGTATTTGGAAATCATGTGTACTTCAACAGTTCAACTCTTCTTAATTACTCGATTCTATGTTCTTCTTTACTGATTGATCGATTTGAAAGGTGGAGGCGAGTTTTTGGTTCGATTACATTGAGAACTAATGACCGGATGTAAGAAAATGGTGGAATCTAGGGAGAAATGGTGATGCTTCTAGTTACATTGAGAGTCAACGAGTTCTTTAGTTTCAAATCTTTGGTGCATCGTATCGATAGTTATTATTTTCATTAATGCTGTTTTTAATTGAAATTAGAAGAATGAGATGCAACCGTGACTTAAAAAATTGTCTGAGGAACTTCGATTTTCATCTTTTTCATTGGATCTCTTTGCAGAAGCCTACGATGCACTTGATCCCAACGGGAACATCACAATCAAATGGGATGTAATGAGCTGGACTCCGGATGGCTATGTTGTAAGTTTCATAGAAGAATTGCAGTTCTTTGGTTTTCTGTGACCAAGCTTTCTACAAACATAATGGTCAATAATGGGGGATACTAGACTATAAATCTTTTATTTGTAAAAAATGTGTTAAGGAGATTATCAGTACCTCTATCATTTGTTTGTTTAGTCTTTGCACTTTTTATTGCCTACTCTAGTGTATTACTAGGTGTAGTGTTTATGAGGATATGCTATAAAATGGAAACTCCTCATATCTGAAGTATATATATATATATATAATAAAATATATATTTGATCGTTTGTTTTTTTCCACCAATAAGTTCCTCTAGTGTTTGTTAGCTCATTTTCCCTCTTTCGTATTGATTCTCTGATCTAAAGGGTTATCTATTTGGTGGCAGGCTGTTGTTACCATGTACAACTTCCAGCAATACCGCCATATTCAAGCTCCAGGTTGGACATTGGGGTGGACATGGGCAAAGAAAGAAGTAATTTGGAGCATGATGGGAGGCCAAACAACAGAACAGGGTGACTGTTCAAGATTCAAAGGAAATACTCCACATTGCTGCAAGAAAGATCCAACCGTTGTGGATTTATTGCCAGGAACTCCTTACAATCAGCAGATTGCAAATTGTTGCAAAGGTGGAGTGATAAATTCCTGGGCCCAAGACCCAGCCAATGCAGCTAGTTCATTTCAAGTCAGTGTTGGTGCTGCAGGAACAAGTAACAAAACTGTCAGACTCCCCAAAAACTTCACTCTTAAAGCGCCAGGACCTGGATACACTTGTGGTCCGGCAAAGATTGTGAAGCCAACCAAATTTATGTCTGGAGATAAAAGAAGAATAACTCAAGCTTTGAGTAAGTAAACTTGCCGTAGATTTGCACATTAAATATTAGGTTCAATGTAGAATTCTAGTGTTGTCATTTGTCAACATGCACATAGATTTCATTAACAATATGAACTTAGGTTGTTTCTGTCTGAGACTTTATAGATCGAATTGGGGTTTAATATTGAATTTGTTGGTTGTTGAACAGTCATATATTCATCATTATTAAATTTGACTTGATATCTGACTAAATGCAAGTTGTAAGTTTGTAACTATGTAATTTACATGTGAGCATGAACACAAGATATCAATATCAGTTAACGGCATTTGATTGAATCTGTCCTTGCTTGTCCTATGTCAGTGACTTGGAATGTTACATGCACCTATTCTCAATTCCTTGCTCAGAAGACACCCACTTGTTGTGTTTCCCTCTCCTCCTTCTACAATGATACCATCGTCAACTGCCCAACCTGCACTTGTGGATGTCAAAACAACACGGCGGGTCCTGGGAGCTGTGTGGAGTAAGTTATTTTTTGCTGTACATCAGGCTCTGTGTTGTTTATTTTTCTTTCAACATTGTGTTTGTGGTTAGGGTGAAAGATTGAATCTATTGTACTCAACTCAATAATCTGTTTCTGCTTAATTTTTTGCAGTCCAGATACTCCACATTTAGCTTCAGTAGTATCAACTCCTGATAAATCGGGCAACATTGAACCCCTGGTTCAGTGTACTAGCCACATGTGTCCCATTCGAGTCCATTGGCATGTGAAGCTGAATTACAAGGAGTACTGGAGGGTGAAGATCACAATCACCAATTTCAATTACAGAATGAACTACACACTCTGGAATCTCGTGGTGCAGCATCCCAATTTTGATAATGTAACTCAATTATTCAGCTTTAACTATAAGTCGTTGAGCCCATATGGTGACCTAAGTAAGTTCTCTTCTCTCTTGGAAATATATTTACATATAATGTTGACCATTTGTTTGGAAAGAACCGGCTTTATGCAATCTATTTTCTCTACTTTGTGGTTGGTGAGGTAACTATGGTACTGTTTTGCAGATGATACTGCCATGCTATGGGGATTTAAGTTCTACAATGATCTGCTCAATCAAGCTGGCCCTCTTGGTAATGTGCAGTCTGAGCTCCTATTCCGGAAAGACCAGAACACTTTCACTTTTGACAAGGGTTGGGCTTTTCCAAGAAGAGTTTATTTCAATGGTGACAACTGTGTGATGCCACCTCCAGATGCCTATCCCTATTTGCCAAATGCCAGTTCCCGACCAGCTATATCTCTACTCATGACAATCTTAATATCTTTGGCATTATTGTTCAATTTTGAACTATAA

mRNA sequence

ATGAGGTCTCGGCGCTTCGCTTCCACTGGATCCGTCGCCGAACTTACCGGCTTTTCCGTCGTGCTTCTCTTTCTACTTTCCAGCTTCAGTTTCACTTCAACAGAAGCCTACGATGCACTTGATCCCAACGGGAACATCACAATCAAATGGGATGTAATGAGCTGGACTCCGGATGGCTATGTTGCTGTTGTTACCATGTACAACTTCCAGCAATACCGCCATATTCAAGCTCCAGGTTGGACATTGGGGTGGACATGGGCAAAGAAAGAAGTAATTTGGAGCATGATGGGAGGCCAAACAACAGAACAGGGTGACTGTTCAAGATTCAAAGGAAATACTCCACATTGCTGCAAGAAAGATCCAACCGTTGTGGATTTATTGCCAGGAACTCCTTACAATCAGCAGATTGCAAATTGTTGCAAAGGTGGAGTGATAAATTCCTGGGCCCAAGACCCAGCCAATGCAGCTAGTTCATTTCAAGTCAGTGTTGGTGCTGCAGGAACAAGTAACAAAACTGTCAGACTCCCCAAAAACTTCACTCTTAAAGCGCCAGGACCTGGATACACTTGTGGTCCGGCAAAGATTGTGAAGCCAACCAAATTTATGTCTGGAGATAAAAGAAGAATAACTCAAGCTTTGATGACTTGGAATGTTACATGCACCTATTCTCAATTCCTTGCTCAGAAGACACCCACTTGTTGTGTTTCCCTCTCCTCCTTCTACAATGATACCATCGTCAACTGCCCAACCTGCACTTGTGGATGTCAAAACAACACGGCGGGTCCTGGGAGCTGTGTGGATCCAGATACTCCACATTTAGCTTCAGTAGTATCAACTCCTGATAAATCGGGCAACATTGAACCCCTGGTTCAGTGTACTAGCCACATGTGTCCCATTCGAGTCCATTGGCATGTGAAGCTGAATTACAAGGAGTACTGGAGGGTGAAGATCACAATCACCAATTTCAATTACAGAATGAACTACACACTCTGGAATCTCGTGGTGCAGCATCCCAATTTTGATAATGTAACTCAATTATTCAGCTTTAACTATAAGTCGTTGAGCCCATATGGTGACCTAAATGATACTGCCATGCTATGGGGATTTAAGTTCTACAATGATCTGCTCAATCAAGCTGGCCCTCTTGGTAATGTGCAGTCTGAGCTCCTATTCCGGAAAGACCAGAACACTTTCACTTTTGACAAGGGTTGGGCTTTTCCAAGAAGAGTTTATTTCAATGGTGACAACTGTGTGATGCCACCTCCAGATGCCTATCCCTATTTGCCAAATGCCAGTTCCCGACCAGCTATATCTCTACTCATGACAATCTTAATATCTTTGGCATTATTGTTCAATTTTGAACTATAA

Coding sequence (CDS)

ATGAGGTCTCGGCGCTTCGCTTCCACTGGATCCGTCGCCGAACTTACCGGCTTTTCCGTCGTGCTTCTCTTTCTACTTTCCAGCTTCAGTTTCACTTCAACAGAAGCCTACGATGCACTTGATCCCAACGGGAACATCACAATCAAATGGGATGTAATGAGCTGGACTCCGGATGGCTATGTTGCTGTTGTTACCATGTACAACTTCCAGCAATACCGCCATATTCAAGCTCCAGGTTGGACATTGGGGTGGACATGGGCAAAGAAAGAAGTAATTTGGAGCATGATGGGAGGCCAAACAACAGAACAGGGTGACTGTTCAAGATTCAAAGGAAATACTCCACATTGCTGCAAGAAAGATCCAACCGTTGTGGATTTATTGCCAGGAACTCCTTACAATCAGCAGATTGCAAATTGTTGCAAAGGTGGAGTGATAAATTCCTGGGCCCAAGACCCAGCCAATGCAGCTAGTTCATTTCAAGTCAGTGTTGGTGCTGCAGGAACAAGTAACAAAACTGTCAGACTCCCCAAAAACTTCACTCTTAAAGCGCCAGGACCTGGATACACTTGTGGTCCGGCAAAGATTGTGAAGCCAACCAAATTTATGTCTGGAGATAAAAGAAGAATAACTCAAGCTTTGATGACTTGGAATGTTACATGCACCTATTCTCAATTCCTTGCTCAGAAGACACCCACTTGTTGTGTTTCCCTCTCCTCCTTCTACAATGATACCATCGTCAACTGCCCAACCTGCACTTGTGGATGTCAAAACAACACGGCGGGTCCTGGGAGCTGTGTGGATCCAGATACTCCACATTTAGCTTCAGTAGTATCAACTCCTGATAAATCGGGCAACATTGAACCCCTGGTTCAGTGTACTAGCCACATGTGTCCCATTCGAGTCCATTGGCATGTGAAGCTGAATTACAAGGAGTACTGGAGGGTGAAGATCACAATCACCAATTTCAATTACAGAATGAACTACACACTCTGGAATCTCGTGGTGCAGCATCCCAATTTTGATAATGTAACTCAATTATTCAGCTTTAACTATAAGTCGTTGAGCCCATATGGTGACCTAAATGATACTGCCATGCTATGGGGATTTAAGTTCTACAATGATCTGCTCAATCAAGCTGGCCCTCTTGGTAATGTGCAGTCTGAGCTCCTATTCCGGAAAGACCAGAACACTTTCACTTTTGACAAGGGTTGGGCTTTTCCAAGAAGAGTTTATTTCAATGGTGACAACTGTGTGATGCCACCTCCAGATGCCTATCCCTATTTGCCAAATGCCAGTTCCCGACCAGCTATATCTCTACTCATGACAATCTTAATATCTTTGGCATTATTGTTCAATTTTGAACTATAA

Protein sequence

MRSRRFASTGSVAELTGFSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGYVAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKDPTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFTLKAPGPGYTCGPAKIVKPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSFYNDTIVNCPTCTCGCQNNTAGPGSCVDPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIRVHWHVKLNYKEYWRVKITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDLNDTAMLWGFKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMPPPDAYPYLPNASSRPAISLLMTILISLALLFNFEL
Homology
BLAST of Spg022745 vs. NCBI nr
Match: XP_038878869.1 (protein COBRA-like [Benincasa hispida])

HSP 1 Score: 920.6 bits (2378), Expect = 5.2e-264
Identity = 432/453 (95.36%), Postives = 445/453 (98.23%), Query Frame = 0

Query: 1   MRSRRFASTGSVAELTGFSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60
           MRSRRFASTGSVA++TGF+V+LLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY
Sbjct: 1   MRSRRFASTGSVADITGFAVLLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60

Query: 61  VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKD 120
           VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKD
Sbjct: 61  VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKD 120

Query: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFT 180
           PTVVDLLPGTPYNQQIANCCKGGVINSWAQDP NAASSFQVSVGAAGTSNKTVRLPKNFT
Sbjct: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPGNAASSFQVSVGAAGTSNKTVRLPKNFT 180

Query: 181 LKAPGPGYTCGPAKIVKPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240
           LKAPGPGYTCGPAKIVKPTKF+SGDKRR+TQALMTWNVTCTYSQFLAQKTPTCCVSLSSF
Sbjct: 181 LKAPGPGYTCGPAKIVKPTKFVSGDKRRVTQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240

Query: 241 YNDTIVNCPTCTCGCQNNTAGPGSCVDPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIR 300
           YNDTIVNCPTCTCGCQNNTAG GSCVDPD+P+LASVVS PDKSGN  PLVQCTSHMCPIR
Sbjct: 241 YNDTIVNCPTCTCGCQNNTAGSGSCVDPDSPYLASVVSAPDKSGNNAPLVQCTSHMCPIR 300

Query: 301 VHWHVKLNYKEYWRVKITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDL 360
           VHWHVKLNYK+YWRVKITITNFNYRMNYTLWNLVVQHPNFDN+T+LFSFNYKSLSPYGDL
Sbjct: 301 VHWHVKLNYKDYWRVKITITNFNYRMNYTLWNLVVQHPNFDNITKLFSFNYKSLSPYGDL 360

Query: 361 NDTAMLWGFKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420
           NDTAMLWG KFYNDLLNQAGPLGNVQSELLF+KDQNTFTFDKGWAFPRRVYFNGDNCVMP
Sbjct: 361 NDTAMLWGVKFYNDLLNQAGPLGNVQSELLFQKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420

Query: 421 PPDAYPYLPNASSRPAISLLMTILISLALLFNF 454
           PPDAYPYLPNASSR AISLL+TILISLALL NF
Sbjct: 421 PPDAYPYLPNASSRSAISLLVTILISLALLLNF 453

BLAST of Spg022745 vs. NCBI nr
Match: XP_022933389.1 (protein COBRA-like [Cucurbita moschata] >KAG6587595.1 Protein COBRA, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 918.3 bits (2372), Expect = 2.6e-263
Identity = 430/455 (94.51%), Postives = 443/455 (97.36%), Query Frame = 0

Query: 1   MRSRRFASTGSVAELTGFSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60
           MRSR F+STGSVAELTGF ++LLFLLS FSFTSTEAYDALDPNGNITIKWDVMSWTPDGY
Sbjct: 1   MRSRLFSSTGSVAELTGFGILLLFLLSCFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60

Query: 61  VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKD 120
           VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMG QTTEQGDCSRFKGNTPHCCKKD
Sbjct: 61  VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGAQTTEQGDCSRFKGNTPHCCKKD 120

Query: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFT 180
           PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTV+LPKNFT
Sbjct: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVKLPKNFT 180

Query: 181 LKAPGPGYTCGPAKIVKPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240
           LKAPGPGYTCGPAKIVK TKF+SGDKRR+TQALMTWNVTCTYSQFLAQKTPTCCVSLSSF
Sbjct: 181 LKAPGPGYTCGPAKIVKSTKFISGDKRRVTQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240

Query: 241 YNDTIVNCPTCTCGCQNNTAGPGSCVDPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIR 300
           YNDTIVNCPTCTCGCQNNTAG GSCV+PD+PHLASVVSTPDKSGN EPLVQCTSHMCPIR
Sbjct: 241 YNDTIVNCPTCTCGCQNNTAGSGSCVNPDSPHLASVVSTPDKSGNYEPLVQCTSHMCPIR 300

Query: 301 VHWHVKLNYKEYWRVKITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDL 360
           VHWHVKLNYK+YWRVKITITNFNYRMNYTLWNLV QHPNFDN+TQLFSFNYKSLSPYGDL
Sbjct: 301 VHWHVKLNYKDYWRVKITITNFNYRMNYTLWNLVAQHPNFDNITQLFSFNYKSLSPYGDL 360

Query: 361 NDTAMLWGFKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420
           NDTAMLWG KFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP
Sbjct: 361 NDTAMLWGVKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420

Query: 421 PPDAYPYLPNASSRPAISLLMTILISLALLFNFEL 456
           PP+AYPYLPNASSRPA+SLL T+LISLALL NF L
Sbjct: 421 PPEAYPYLPNASSRPAMSLLATVLISLALLLNFAL 455

BLAST of Spg022745 vs. NCBI nr
Match: KAG7021561.1 (Protein COBRA, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 918.3 bits (2372), Expect = 2.6e-263
Identity = 430/455 (94.51%), Postives = 443/455 (97.36%), Query Frame = 0

Query: 1   MRSRRFASTGSVAELTGFSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60
           MRSR F+STGSVAELTGF ++LLFLLS FSFTSTEAYDALDPNGNITIKWDVMSWTPDGY
Sbjct: 53  MRSRLFSSTGSVAELTGFGILLLFLLSCFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 112

Query: 61  VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKD 120
           VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMG QTTEQGDCSRFKGNTPHCCKKD
Sbjct: 113 VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGAQTTEQGDCSRFKGNTPHCCKKD 172

Query: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFT 180
           PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTV+LPKNFT
Sbjct: 173 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVKLPKNFT 232

Query: 181 LKAPGPGYTCGPAKIVKPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240
           LKAPGPGYTCGPAKIVK TKF+SGDKRR+TQALMTWNVTCTYSQFLAQKTPTCCVSLSSF
Sbjct: 233 LKAPGPGYTCGPAKIVKSTKFISGDKRRVTQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 292

Query: 241 YNDTIVNCPTCTCGCQNNTAGPGSCVDPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIR 300
           YNDTIVNCPTCTCGCQNNTAG GSCV+PD+PHLASVVSTPDKSGN EPLVQCTSHMCPIR
Sbjct: 293 YNDTIVNCPTCTCGCQNNTAGSGSCVNPDSPHLASVVSTPDKSGNYEPLVQCTSHMCPIR 352

Query: 301 VHWHVKLNYKEYWRVKITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDL 360
           VHWHVKLNYK+YWRVKITITNFNYRMNYTLWNLV QHPNFDN+TQLFSFNYKSLSPYGDL
Sbjct: 353 VHWHVKLNYKDYWRVKITITNFNYRMNYTLWNLVAQHPNFDNITQLFSFNYKSLSPYGDL 412

Query: 361 NDTAMLWGFKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420
           NDTAMLWG KFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP
Sbjct: 413 NDTAMLWGVKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP 472

Query: 421 PPDAYPYLPNASSRPAISLLMTILISLALLFNFEL 456
           PP+AYPYLPNASSRPA+SLL T+LISLALL NF L
Sbjct: 473 PPEAYPYLPNASSRPAMSLLATVLISLALLLNFAL 507

BLAST of Spg022745 vs. NCBI nr
Match: XP_023531135.1 (protein COBRA-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 917.1 bits (2369), Expect = 5.8e-263
Identity = 429/455 (94.29%), Postives = 443/455 (97.36%), Query Frame = 0

Query: 1   MRSRRFASTGSVAELTGFSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60
           MRSR F+STGSVAELTGF ++LLFLLS FSFTSTEAYDALDPNGNITIKWDVMSWTPDGY
Sbjct: 1   MRSRLFSSTGSVAELTGFGLLLLFLLSCFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60

Query: 61  VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKD 120
           VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMG QTTEQGDCSRFKGNTPHCCKKD
Sbjct: 61  VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGAQTTEQGDCSRFKGNTPHCCKKD 120

Query: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFT 180
           PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTV+LPKNFT
Sbjct: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVKLPKNFT 180

Query: 181 LKAPGPGYTCGPAKIVKPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240
           LKAPGPGYTCGPAKIVK TKF+SGDKRR+TQALMTWNVTCTYSQFLAQKTPTCCVSLSSF
Sbjct: 181 LKAPGPGYTCGPAKIVKSTKFISGDKRRVTQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240

Query: 241 YNDTIVNCPTCTCGCQNNTAGPGSCVDPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIR 300
           YNDTIVNCPTCTCGCQNNTAG GSCV+PD+PHLASVVSTPDKSGN EPL+QCTSHMCPIR
Sbjct: 241 YNDTIVNCPTCTCGCQNNTAGSGSCVNPDSPHLASVVSTPDKSGNYEPLIQCTSHMCPIR 300

Query: 301 VHWHVKLNYKEYWRVKITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDL 360
           VHWHVKLNYK+YWRVKITITNFNYRMNYTLWNLV QHPNFDN+TQLFSFNYKSLSPYGDL
Sbjct: 301 VHWHVKLNYKDYWRVKITITNFNYRMNYTLWNLVAQHPNFDNITQLFSFNYKSLSPYGDL 360

Query: 361 NDTAMLWGFKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420
           NDTAMLWG KFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP
Sbjct: 361 NDTAMLWGVKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420

Query: 421 PPDAYPYLPNASSRPAISLLMTILISLALLFNFEL 456
           PP+AYPYLPNASSRPA+SLL T+LISLALL NF L
Sbjct: 421 PPEAYPYLPNASSRPAMSLLATVLISLALLLNFAL 455

BLAST of Spg022745 vs. NCBI nr
Match: XP_022966079.1 (protein COBRA-like [Cucurbita maxima])

HSP 1 Score: 914.8 bits (2363), Expect = 2.9e-262
Identity = 429/455 (94.29%), Postives = 442/455 (97.14%), Query Frame = 0

Query: 1   MRSRRFASTGSVAELTGFSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60
           MRSR F+STGSVAELTGF ++LLFLLS FSFTSTEAYDALDPNGNITIKWDVMSWTPDGY
Sbjct: 1   MRSRPFSSTGSVAELTGFGLLLLFLLSCFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60

Query: 61  VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKD 120
           VAVVTMYNFQQYRHIQAPGWT+GWTWAKKEVIWSMMG QTTEQGDCSRFKGNTPHCCKKD
Sbjct: 61  VAVVTMYNFQQYRHIQAPGWTVGWTWAKKEVIWSMMGAQTTEQGDCSRFKGNTPHCCKKD 120

Query: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFT 180
           PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTV+LPKNFT
Sbjct: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVKLPKNFT 180

Query: 181 LKAPGPGYTCGPAKIVKPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240
           LKAPGPGYTCGPAKIVK TKF+SGDKRR+TQALMTWNVTCTYSQFLAQKTPTCCVSLSSF
Sbjct: 181 LKAPGPGYTCGPAKIVKSTKFISGDKRRVTQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240

Query: 241 YNDTIVNCPTCTCGCQNNTAGPGSCVDPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIR 300
           YNDTIVNCPTCTCGCQNNTAG GSCV+PD+PHLASVVSTPDKSGN EPLVQCTSHMCPIR
Sbjct: 241 YNDTIVNCPTCTCGCQNNTAGSGSCVNPDSPHLASVVSTPDKSGNYEPLVQCTSHMCPIR 300

Query: 301 VHWHVKLNYKEYWRVKITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDL 360
           VHWHVKLNYK+YWRVKITITNFNYRMNYTLWNLV QHPNFDN+TQLFSFNYKSLSPYGDL
Sbjct: 301 VHWHVKLNYKDYWRVKITITNFNYRMNYTLWNLVAQHPNFDNITQLFSFNYKSLSPYGDL 360

Query: 361 NDTAMLWGFKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420
           NDTAMLWG KFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP
Sbjct: 361 NDTAMLWGVKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420

Query: 421 PPDAYPYLPNASSRPAISLLMTILISLALLFNFEL 456
           PP+AYPYLPNASSRPA+SLL T LISLALL NF L
Sbjct: 421 PPEAYPYLPNASSRPAMSLLATALISLALLLNFAL 455

BLAST of Spg022745 vs. ExPASy Swiss-Prot
Match: Q94KT8 (Protein COBRA OS=Arabidopsis thaliana OX=3702 GN=COB PE=2 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 7.2e-224
Identity = 351/447 (78.52%), Postives = 397/447 (88.81%), Query Frame = 0

Query: 8   STGSVAELTGFSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGYVAVVTMY 67
           ST  V++L+  ++ ++FL+SS SFTSTEAYDALDP GNIT+KWDVMSWTPDGYVAVVTM+
Sbjct: 8   STSIVSKLSFLALWIVFLISSSSFTSTEAYDALDPEGNITMKWDVMSWTPDGYVAVVTMF 67

Query: 68  NFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKDPTVVDLL 127
           NFQ+YRHIQ+PGWTLGW WAKKEVIWSM+G QTTEQGDCS++KGN PHCCKKDPTVVDLL
Sbjct: 68  NFQKYRHIQSPGWTLGWKWAKKEVIWSMVGAQTTEQGDCSKYKGNIPHCCKKDPTVVDLL 127

Query: 128 PGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFTLKAPGPG 187
           PGTPYNQQIANCCKGGV+NSW QDPA AASSFQ+SVGAAGT+NKTVR+P+NFTL  PGPG
Sbjct: 128 PGTPYNQQIANCCKGGVMNSWVQDPATAASSFQISVGAAGTTNKTVRVPRNFTLMGPGPG 187

Query: 188 YTCGPAKIVKPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSFYNDTIVN 247
           YTCGPAKIV+PTKF++ D RR TQA+MTWN+TCTYSQFLAQ+TPTCCVSLSSFYN+TIV 
Sbjct: 188 YTCGPAKIVRPTKFVTTDTRRTTQAMMTWNITCTYSQFLAQRTPTCCVSLSSFYNETIVG 247

Query: 248 CPTCTCGCQNNTAGPGSCVDPDTPHLASVVSTPDKSGNI-EPLVQCTSHMCPIRVHWHVK 307
           CPTC CGCQNN    G+C+DPDTPHLASVVS P K G +  PLVQCT HMCPIRVHWHVK
Sbjct: 248 CPTCACGCQNNRTESGACLDPDTPHLASVVSPPTKKGTVLPPLVQCTRHMCPIRVHWHVK 307

Query: 308 LNYKEYWRVKITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDLNDTAML 367
            NYKEYWRVKITITNFNYR+NYT WNLV QHPN DN+TQ+FSFNYKSL+PY  LNDTAML
Sbjct: 308 QNYKEYWRVKITITNFNYRLNYTQWNLVAQHPNLDNITQIFSFNYKSLTPYAGLNDTAML 367

Query: 368 WGFKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMPPPDAYP 427
           WG KFYND L++AGPLGNVQSE+LFRKDQ+TFTF+KGWAFPRR+YFNGDNCVMPPPD+YP
Sbjct: 368 WGVKFYNDFLSEAGPLGNVQSEILFRKDQSTFTFEKGWAFPRRIYFNGDNCVMPPPDSYP 427

Query: 428 YLPNASSRPAISLLMTILISLALLFNF 454
           +LPN  SR   S +  +L+ L + F F
Sbjct: 428 FLPNGGSRSQFSFVAAVLLPLLVFFFF 454

BLAST of Spg022745 vs. ExPASy Swiss-Prot
Match: Q60E70 (COBRA-like protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=BC1L4 PE=2 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 1.8e-211
Identity = 343/439 (78.13%), Postives = 379/439 (86.33%), Query Frame = 0

Query: 8   STGSVAELTGFSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGYVAVVTMY 67
           S+ SVA     +V+L   L   +  +TEAYDALDPNGNITIKWDVMSWTPDGYVAVVTM+
Sbjct: 8   SSRSVAPCCCCAVLLAAALLFSAPATTEAYDALDPNGNITIKWDVMSWTPDGYVAVVTMF 67

Query: 68  NFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKDPTVVDLL 127
           N+QQ+RHIQAPGW LGWTWAKKEVIWSM+G QTTEQGDCS+FKG TPHCCKKDPTVVDLL
Sbjct: 68  NYQQFRHIQAPGWQLGWTWAKKEVIWSMVGAQTTEQGDCSKFKGGTPHCCKKDPTVVDLL 127

Query: 128 PGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFTLKAPGPG 187
           PGTPYN QIANCCK GVIN++ QDP+NAASSFQ+SVG AGT+NKTV+LPKNFTLKAPGPG
Sbjct: 128 PGTPYNMQIANCCKAGVINTFNQDPSNAASSFQISVGLAGTTNKTVKLPKNFTLKAPGPG 187

Query: 188 YTCGPAKIVKPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSFYNDTIVN 247
           YTCG A IV+PTKF +GD RR TQALMTWNVTCTYSQFLAQKTP+CCVSLSSFYNDTIVN
Sbjct: 188 YTCGRAMIVRPTKFFTGDGRRATQALMTWNVTCTYSQFLAQKTPSCCVSLSSFYNDTIVN 247

Query: 248 CPTCTCGCQNNTAGPGSCVDPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIRVHWHVKL 307
           CPTC+CGCQNN   PGSCV+ ++P+L S +  P K    +PLVQCTSHMCPIR+HWHVKL
Sbjct: 248 CPTCSCGCQNNGTSPGSCVNENSPYLQSAIDGPGKWTG-QPLVQCTSHMCPIRIHWHVKL 307

Query: 308 NYKEYWRVKITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGD-LNDTAML 367
           NYKEYWRVKITITNFNYRMNYT WNLV QHPNF+N+TQLFSFNYK L+PYG  +NDTAM 
Sbjct: 308 NYKEYWRVKITITNFNYRMNYTQWNLVAQHPNFNNITQLFSFNYKPLTPYGSKINDTAMF 367

Query: 368 WGFKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMPPPDAYP 427
           WG KFYNDLL QAGPLGN QSELL RKD   FTFDKGWAFP RVYFNGDNCVMPPPDAYP
Sbjct: 368 WGVKFYNDLLMQAGPLGNAQSELLLRKDSKDFTFDKGWAFPHRVYFNGDNCVMPPPDAYP 427

Query: 428 YLPNASSRPAISLLMTILI 446
           +LPNAS      L +++L+
Sbjct: 428 WLPNASPLTKQPLTLSVLV 445

BLAST of Spg022745 vs. ExPASy Swiss-Prot
Match: Q9SRT7 (COBRA-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=COBL1 PE=2 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 3.2e-208
Identity = 320/434 (73.73%), Postives = 376/434 (86.64%), Query Frame = 0

Query: 18  FSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGYVAVVTMYNFQQYRHIQA 77
           F + ++FL+S    T +EAYD LDP+GNIT+KWD+++WT DGYVA VT+YNFQQYRHIQA
Sbjct: 15  FGISIIFLVSFSGLTPSEAYDPLDPSGNITVKWDIITWTGDGYVATVTVYNFQQYRHIQA 74

Query: 78  PGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKDPTVVDLLPGTPYNQQIA 137
           PGWTLGW+WAK+EVIW M GGQTTEQGDCS+FKG  PHCCKK P+VVDLLPG+PYNQQIA
Sbjct: 75  PGWTLGWSWAKREVIWGMNGGQTTEQGDCSKFKGTIPHCCKKTPSVVDLLPGSPYNQQIA 134

Query: 138 NCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFTLKAPGPGYTCGPAKIVK 197
           NCC+GGV+NSWAQDPA A S+FQ++VG AGT+NKTVR+PKNFTLKAPGPGYTC PAKIVK
Sbjct: 135 NCCRGGVLNSWAQDPATAVSAFQLTVGQAGTTNKTVRVPKNFTLKAPGPGYTCSPAKIVK 194

Query: 198 PTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSFYNDTIVNCPTCTCGCQN 257
           PT+F+  DKRR+TQALMTWNVTCTYSQFLAQKTPTCCVSLSSFYN TIV+CPTC+CGC+ 
Sbjct: 195 PTRFIGTDKRRVTQALMTWNVTCTYSQFLAQKTPTCCVSLSSFYNKTIVSCPTCSCGCR- 254

Query: 258 NTAGPGSCVDPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIRVHWHVKLNYKEYWRVKI 317
           NT+ PG+CVDP  P +ASV+  P K+  I PLVQCT HMCP+R+HWHVK+NYK+YWRVK+
Sbjct: 255 NTSQPGNCVDPKGPRIASVIPNPGKNAYIPPLVQCTKHMCPVRIHWHVKVNYKQYWRVKV 314

Query: 318 TITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDLNDTAMLWGFKFYNDLLN 377
           TITNFNY MNY+ WNLVVQHPNFDN+TQ FSFNYK L+PY  +NDT +LWG KFYNDLL 
Sbjct: 315 TITNFNYNMNYSQWNLVVQHPNFDNLTQTFSFNYKPLTPYASINDTGILWGIKFYNDLLM 374

Query: 378 QAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMPPPDAYPYLPNASSRPAI 437
           QAGP GNVQSELLF+K+ + FTF+KGWAFPRR+YFNGDNCVMPPPD+YP+LPN  S  ++
Sbjct: 375 QAGPFGNVQSELLFQKEASAFTFEKGWAFPRRIYFNGDNCVMPPPDSYPWLPNTGSHKSV 434

Query: 438 SLLMTILISLALLF 452
             L   +  L ++F
Sbjct: 435 GSLFAAMALLLIVF 447

BLAST of Spg022745 vs. ExPASy Swiss-Prot
Match: Q6Z4G8 (COBRA-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BC1L6 PE=2 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 1.2e-207
Identity = 334/436 (76.61%), Postives = 380/436 (87.16%), Query Frame = 0

Query: 17  GFSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGYVAVVTMYNFQQYRHIQ 76
           G SV LL    S    S+EAYD LDPNGNITIKWDV+ WTPDGYVAVV++YN+QQYRHIQ
Sbjct: 9   GVSVALLVAFFSSLIPSSEAYDPLDPNGNITIKWDVLQWTPDGYVAVVSLYNYQQYRHIQ 68

Query: 77  APGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKDPTVVDLLPGTPYNQQI 136
           +PGW LGW WAKKE+IW+M GGQ TEQGDCS+FK N PHCCKKDP +VDLLPGTPYN QI
Sbjct: 69  SPGWKLGWVWAKKEIIWAMNGGQATEQGDCSKFKSNIPHCCKKDPEIVDLLPGTPYNMQI 128

Query: 137 ANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFTLKAPGPGYTCGPAKIV 196
           ANCCKGGV+NSWAQDPANA +SFQVSVG AGT+NKTVR+P+NFTLK+PGPGYTCG AK+V
Sbjct: 129 ANCCKGGVLNSWAQDPANAIASFQVSVGQAGTTNKTVRVPRNFTLKSPGPGYTCGSAKVV 188

Query: 197 KPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSFYNDTIVNCPTCTCGCQ 256
           +PTKF S D RR TQA MTWNVTCTYSQ +AQ++PTCCVSLSSFYNDTIVNCPTC+CGCQ
Sbjct: 189 RPTKFFSQDGRRTTQAHMTWNVTCTYSQIVAQRSPTCCVSLSSFYNDTIVNCPTCSCGCQ 248

Query: 257 NNTAGPGSCVDPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIRVHWHVKLNYKEYWRVK 316
           NN   PGSCV+ ++P+LASVV+T +K  ++ PLVQCTSHMCPIRVHWHVK+NYKEYWRVK
Sbjct: 249 NNK--PGSCVEGNSPYLASVVNTHNKD-SLTPLVQCTSHMCPIRVHWHVKVNYKEYWRVK 308

Query: 317 ITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDLNDTAMLWGFKFYNDLL 376
           IT+TNFNYRMNY+ WNLV QHP+FDN+T +FSFNYKSL+PYG +NDTAMLWG K+YNDLL
Sbjct: 309 ITVTNFNYRMNYSQWNLVTQHPSFDNLTTIFSFNYKSLNPYGVINDTAMLWGIKYYNDLL 368

Query: 377 NQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMPPPDAYPYLPNASSRPA 436
             AGP GNVQSELLF+KD  +FTF+KGWAFPRRVYFNGDNCVMPPPDAYP+LPNAS+R  
Sbjct: 369 MTAGPDGNVQSELLFKKDPKSFTFEKGWAFPRRVYFNGDNCVMPPPDAYPWLPNASTRVM 428

Query: 437 ISLLMT-ILISLALLF 452
            S+L+  I I  AL F
Sbjct: 429 SSILLPFITIWTALTF 441

BLAST of Spg022745 vs. ExPASy Swiss-Prot
Match: Q8L8Q7 (COBRA-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=COBL2 PE=2 SV=2)

HSP 1 Score: 707.6 bits (1825), Expect = 9.1e-203
Identity = 326/439 (74.26%), Postives = 383/439 (87.24%), Query Frame = 0

Query: 18  FSVVLLFLLS--SFSFTSTEAYDALDPNGNITIKWDVMSWTPDGYVAVVTMYNFQQYRHI 77
           FS +LLFL S  SF+FT+TEAYDALDP GNITIKWD+MSWT DGYVAVVT++NFQQYRHI
Sbjct: 8   FSFLLLFLCSWTSFTFTTTEAYDALDPYGNITIKWDIMSWTGDGYVAVVTIFNFQQYRHI 67

Query: 78  QAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKDPTVVDLLPGTPYNQQ 137
           +APGW LGW+W KKEVIWSM+GGQ TEQGDCS+FKGN PHCCKK P +VDLLPGTPYNQQ
Sbjct: 68  EAPGWQLGWSWMKKEVIWSMVGGQATEQGDCSKFKGNIPHCCKKTPAIVDLLPGTPYNQQ 127

Query: 138 IANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFTLKAPGPGYTCGPAKI 197
           I+NCC+GGVI++WAQDPA A SSFQ+SVG +GT+N TVR P+N TLKAPGPGYTCGPAK+
Sbjct: 128 ISNCCRGGVISAWAQDPATAISSFQISVGQSGTTNTTVRAPRNITLKAPGPGYTCGPAKL 187

Query: 198 VKPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSFYNDTIVNCPTCTCGC 257
           VKP++F+S DKRR TQ+L+TWN+TCTYSQFLA+KTPTCCVSLS+FYN+TIV CPTC+CGC
Sbjct: 188 VKPSRFISADKRRKTQSLLTWNITCTYSQFLARKTPTCCVSLSAFYNETIVPCPTCSCGC 247

Query: 258 QNNTAGPGSCVDPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIRVHWHVKLNYKEYWRV 317
           QN++   G+CVD   P +ASVV    K+ N+EPL+QCT HMCPIRVHWHVK +YKEYWRV
Sbjct: 248 QNSSQA-GTCVD---PKIASVVPALGKN-NLEPLLQCTQHMCPIRVHWHVKTSYKEYWRV 307

Query: 318 KITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDLNDTAMLWGFKFYNDL 377
           K+ ITNFNY MNY+ WNLVVQHPNFDN+T+LFSFNYK L+PY ++NDTAMLWG KFYND 
Sbjct: 308 KVAITNFNYNMNYSQWNLVVQHPNFDNLTKLFSFNYKPLNPYLNINDTAMLWGIKFYNDF 367

Query: 378 LNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMPPPDAYPYLPNAS--- 437
           L+QAGP+GNVQSELLF+K+   FTF+KGWAFPRR+YFNGDNCVMPPPD+YP+LPNAS   
Sbjct: 368 LSQAGPVGNVQSELLFQKNPLEFTFEKGWAFPRRIYFNGDNCVMPPPDSYPWLPNASPNI 427

Query: 438 -SRPAISLLMTILISLALL 451
            + P + LL+T L  L L+
Sbjct: 428 ATSPFVILLITFLSVLILM 441

BLAST of Spg022745 vs. ExPASy TrEMBL
Match: A0A6J1EZM8 (COBRA-like protein OS=Cucurbita moschata OX=3662 GN=LOC111440818 PE=3 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 1.3e-263
Identity = 430/455 (94.51%), Postives = 443/455 (97.36%), Query Frame = 0

Query: 1   MRSRRFASTGSVAELTGFSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60
           MRSR F+STGSVAELTGF ++LLFLLS FSFTSTEAYDALDPNGNITIKWDVMSWTPDGY
Sbjct: 1   MRSRLFSSTGSVAELTGFGILLLFLLSCFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60

Query: 61  VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKD 120
           VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMG QTTEQGDCSRFKGNTPHCCKKD
Sbjct: 61  VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGAQTTEQGDCSRFKGNTPHCCKKD 120

Query: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFT 180
           PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTV+LPKNFT
Sbjct: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVKLPKNFT 180

Query: 181 LKAPGPGYTCGPAKIVKPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240
           LKAPGPGYTCGPAKIVK TKF+SGDKRR+TQALMTWNVTCTYSQFLAQKTPTCCVSLSSF
Sbjct: 181 LKAPGPGYTCGPAKIVKSTKFISGDKRRVTQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240

Query: 241 YNDTIVNCPTCTCGCQNNTAGPGSCVDPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIR 300
           YNDTIVNCPTCTCGCQNNTAG GSCV+PD+PHLASVVSTPDKSGN EPLVQCTSHMCPIR
Sbjct: 241 YNDTIVNCPTCTCGCQNNTAGSGSCVNPDSPHLASVVSTPDKSGNYEPLVQCTSHMCPIR 300

Query: 301 VHWHVKLNYKEYWRVKITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDL 360
           VHWHVKLNYK+YWRVKITITNFNYRMNYTLWNLV QHPNFDN+TQLFSFNYKSLSPYGDL
Sbjct: 301 VHWHVKLNYKDYWRVKITITNFNYRMNYTLWNLVAQHPNFDNITQLFSFNYKSLSPYGDL 360

Query: 361 NDTAMLWGFKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420
           NDTAMLWG KFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP
Sbjct: 361 NDTAMLWGVKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420

Query: 421 PPDAYPYLPNASSRPAISLLMTILISLALLFNFEL 456
           PP+AYPYLPNASSRPA+SLL T+LISLALL NF L
Sbjct: 421 PPEAYPYLPNASSRPAMSLLATVLISLALLLNFAL 455

BLAST of Spg022745 vs. ExPASy TrEMBL
Match: A0A6J1HSM9 (COBRA-like protein OS=Cucurbita maxima OX=3661 GN=LOC111465789 PE=3 SV=1)

HSP 1 Score: 914.8 bits (2363), Expect = 1.4e-262
Identity = 429/455 (94.29%), Postives = 442/455 (97.14%), Query Frame = 0

Query: 1   MRSRRFASTGSVAELTGFSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60
           MRSR F+STGSVAELTGF ++LLFLLS FSFTSTEAYDALDPNGNITIKWDVMSWTPDGY
Sbjct: 1   MRSRPFSSTGSVAELTGFGLLLLFLLSCFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60

Query: 61  VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKD 120
           VAVVTMYNFQQYRHIQAPGWT+GWTWAKKEVIWSMMG QTTEQGDCSRFKGNTPHCCKKD
Sbjct: 61  VAVVTMYNFQQYRHIQAPGWTVGWTWAKKEVIWSMMGAQTTEQGDCSRFKGNTPHCCKKD 120

Query: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFT 180
           PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTV+LPKNFT
Sbjct: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVKLPKNFT 180

Query: 181 LKAPGPGYTCGPAKIVKPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240
           LKAPGPGYTCGPAKIVK TKF+SGDKRR+TQALMTWNVTCTYSQFLAQKTPTCCVSLSSF
Sbjct: 181 LKAPGPGYTCGPAKIVKSTKFISGDKRRVTQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240

Query: 241 YNDTIVNCPTCTCGCQNNTAGPGSCVDPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIR 300
           YNDTIVNCPTCTCGCQNNTAG GSCV+PD+PHLASVVSTPDKSGN EPLVQCTSHMCPIR
Sbjct: 241 YNDTIVNCPTCTCGCQNNTAGSGSCVNPDSPHLASVVSTPDKSGNYEPLVQCTSHMCPIR 300

Query: 301 VHWHVKLNYKEYWRVKITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDL 360
           VHWHVKLNYK+YWRVKITITNFNYRMNYTLWNLV QHPNFDN+TQLFSFNYKSLSPYGDL
Sbjct: 301 VHWHVKLNYKDYWRVKITITNFNYRMNYTLWNLVAQHPNFDNITQLFSFNYKSLSPYGDL 360

Query: 361 NDTAMLWGFKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420
           NDTAMLWG KFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP
Sbjct: 361 NDTAMLWGVKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420

Query: 421 PPDAYPYLPNASSRPAISLLMTILISLALLFNFEL 456
           PP+AYPYLPNASSRPA+SLL T LISLALL NF L
Sbjct: 421 PPEAYPYLPNASSRPAMSLLATALISLALLLNFAL 455

BLAST of Spg022745 vs. ExPASy TrEMBL
Match: A0A5D3BHU5 (COBRA-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold346G00030 PE=3 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 7.6e-261
Identity = 426/453 (94.04%), Postives = 442/453 (97.57%), Query Frame = 0

Query: 1   MRSRRFASTGSVAELTGFSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60
           MRS RF STGSVA+L+  +V+ LFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY
Sbjct: 1   MRSWRFVSTGSVADLSVIAVLFLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60

Query: 61  VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKD 120
           VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKD
Sbjct: 61  VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKD 120

Query: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFT 180
           PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTV+LPKNFT
Sbjct: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVKLPKNFT 180

Query: 181 LKAPGPGYTCGPAKIVKPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240
           LKAPGPGYTCGPAKIV+PTKF+SGDKRR+TQALMTWNVTCTYSQFLAQKTPTCCVSLSSF
Sbjct: 181 LKAPGPGYTCGPAKIVRPTKFVSGDKRRVTQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240

Query: 241 YNDTIVNCPTCTCGCQNNTAGPGSCVDPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIR 300
           YNDTIVNCPTCTCGCQNNTAG GSCVDPD+P+LASVVS PDKSGN  PLVQCTSHMCPIR
Sbjct: 241 YNDTIVNCPTCTCGCQNNTAGSGSCVDPDSPYLASVVSAPDKSGNSAPLVQCTSHMCPIR 300

Query: 301 VHWHVKLNYKEYWRVKITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDL 360
           VHWHVKLNYK+YWRVKITITNFNYRMNYTLWNLVVQHPNFDN+T+LFSFNYKSLSPYGDL
Sbjct: 301 VHWHVKLNYKDYWRVKITITNFNYRMNYTLWNLVVQHPNFDNITKLFSFNYKSLSPYGDL 360

Query: 361 NDTAMLWGFKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420
           NDTAMLWG KFYNDLLNQAGPLGNVQSELLF+KDQNTFTFDKGWAFPRRVYFNGDNCVMP
Sbjct: 361 NDTAMLWGVKFYNDLLNQAGPLGNVQSELLFQKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420

Query: 421 PPDAYPYLPNASSRPAISLLMTILISLALLFNF 454
           PP+AYPYLPNASSRPAISLL+TILISLALL NF
Sbjct: 421 PPEAYPYLPNASSRPAISLLVTILISLALLLNF 453

BLAST of Spg022745 vs. ExPASy TrEMBL
Match: A0A1S3BVW7 (COBRA-like protein OS=Cucumis melo OX=3656 GN=LOC103494060 PE=3 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 7.6e-261
Identity = 426/453 (94.04%), Postives = 442/453 (97.57%), Query Frame = 0

Query: 1   MRSRRFASTGSVAELTGFSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60
           MRS RF STGSVA+L+  +V+ LFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY
Sbjct: 1   MRSWRFVSTGSVADLSVIAVLFLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60

Query: 61  VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKD 120
           VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKD
Sbjct: 61  VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKD 120

Query: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFT 180
           PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTV+LPKNFT
Sbjct: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVKLPKNFT 180

Query: 181 LKAPGPGYTCGPAKIVKPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240
           LKAPGPGYTCGPAKIV+PTKF+SGDKRR+TQALMTWNVTCTYSQFLAQKTPTCCVSLSSF
Sbjct: 181 LKAPGPGYTCGPAKIVRPTKFVSGDKRRVTQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240

Query: 241 YNDTIVNCPTCTCGCQNNTAGPGSCVDPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIR 300
           YNDTIVNCPTCTCGCQNNTAG GSCVDPD+P+LASVVS PDKSGN  PLVQCTSHMCPIR
Sbjct: 241 YNDTIVNCPTCTCGCQNNTAGSGSCVDPDSPYLASVVSAPDKSGNSAPLVQCTSHMCPIR 300

Query: 301 VHWHVKLNYKEYWRVKITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDL 360
           VHWHVKLNYK+YWRVKITITNFNYRMNYTLWNLVVQHPNFDN+T+LFSFNYKSLSPYGDL
Sbjct: 301 VHWHVKLNYKDYWRVKITITNFNYRMNYTLWNLVVQHPNFDNITKLFSFNYKSLSPYGDL 360

Query: 361 NDTAMLWGFKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420
           NDTAMLWG KFYNDLLNQAGPLGNVQSELLF+KDQNTFTFDKGWAFPRRVYFNGDNCVMP
Sbjct: 361 NDTAMLWGVKFYNDLLNQAGPLGNVQSELLFQKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420

Query: 421 PPDAYPYLPNASSRPAISLLMTILISLALLFNF 454
           PP+AYPYLPNASSRPAISLL+TILISLALL NF
Sbjct: 421 PPEAYPYLPNASSRPAISLLVTILISLALLLNF 453

BLAST of Spg022745 vs. ExPASy TrEMBL
Match: A0A6J1E1U2 (COBRA-like protein OS=Cucurbita moschata OX=3662 GN=LOC111430062 PE=3 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 1.7e-260
Identity = 424/453 (93.60%), Postives = 442/453 (97.57%), Query Frame = 0

Query: 1   MRSRRFASTGSVAELTGFSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGY 60
           M++RRFASTGSVA LTGF+V+LLFLLSSFSFT+TEAYDALDPNGNITIKWDVMSWTPDGY
Sbjct: 1   MKARRFASTGSVAGLTGFAVLLLFLLSSFSFTATEAYDALDPNGNITIKWDVMSWTPDGY 60

Query: 61  VAVVTMYNFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKD 120
           VAVVTMYNFQQYRHI APGWTLGWTWAKKEVIWSMMG QTTEQGDCS+FKGN PHCCKKD
Sbjct: 61  VAVVTMYNFQQYRHIAAPGWTLGWTWAKKEVIWSMMGAQTTEQGDCSKFKGNIPHCCKKD 120

Query: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFT 180
           PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVG AGTSNKTV+LPKNFT
Sbjct: 121 PTVVDLLPGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGGAGTSNKTVKLPKNFT 180

Query: 181 LKAPGPGYTCGPAKIVKPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSF 240
           LKAPGPGYTCGPAKIVKPT+F+SGDKRRITQALMTWN+TCTYSQFLAQKTPTCCVSLSSF
Sbjct: 181 LKAPGPGYTCGPAKIVKPTRFVSGDKRRITQALMTWNITCTYSQFLAQKTPTCCVSLSSF 240

Query: 241 YNDTIVNCPTCTCGCQNNTAGPGSCVDPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIR 300
           YNDTIVNCPTCTCGCQNNTAG GSCVDPD+P+LASVVSTPDKSG+  PLVQCTSHMCPIR
Sbjct: 241 YNDTIVNCPTCTCGCQNNTAGSGSCVDPDSPYLASVVSTPDKSGSNAPLVQCTSHMCPIR 300

Query: 301 VHWHVKLNYKEYWRVKITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDL 360
           VHWHVKLNYK+YWRVKITITNFNYRMNYTLWNLVVQHPNFDN+T+LFSFNYKSLSPYGDL
Sbjct: 301 VHWHVKLNYKDYWRVKITITNFNYRMNYTLWNLVVQHPNFDNITKLFSFNYKSLSPYGDL 360

Query: 361 NDTAMLWGFKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMP 420
           NDTAMLWG KFYNDLLNQAGPLGNVQSELLF+KDQNTFTFDKGWAFPRRVYFNGDNCV+P
Sbjct: 361 NDTAMLWGVKFYNDLLNQAGPLGNVQSELLFQKDQNTFTFDKGWAFPRRVYFNGDNCVLP 420

Query: 421 PPDAYPYLPNASSRPAISLLMTILISLALLFNF 454
           PPDAYPYLPNASSRPAISLLMTILISL LL NF
Sbjct: 421 PPDAYPYLPNASSRPAISLLMTILISLTLLLNF 453

BLAST of Spg022745 vs. TAIR 10
Match: AT5G60920.1 (COBRA-like extracellular glycosyl-phosphatidyl inositol-anchored protein family )

HSP 1 Score: 777.7 bits (2007), Expect = 5.1e-225
Identity = 351/447 (78.52%), Postives = 397/447 (88.81%), Query Frame = 0

Query: 8   STGSVAELTGFSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGYVAVVTMY 67
           ST  V++L+  ++ ++FL+SS SFTSTEAYDALDP GNIT+KWDVMSWTPDGYVAVVTM+
Sbjct: 8   STSIVSKLSFLALWIVFLISSSSFTSTEAYDALDPEGNITMKWDVMSWTPDGYVAVVTMF 67

Query: 68  NFQQYRHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKDPTVVDLL 127
           NFQ+YRHIQ+PGWTLGW WAKKEVIWSM+G QTTEQGDCS++KGN PHCCKKDPTVVDLL
Sbjct: 68  NFQKYRHIQSPGWTLGWKWAKKEVIWSMVGAQTTEQGDCSKYKGNIPHCCKKDPTVVDLL 127

Query: 128 PGTPYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFTLKAPGPG 187
           PGTPYNQQIANCCKGGV+NSW QDPA AASSFQ+SVGAAGT+NKTVR+P+NFTL  PGPG
Sbjct: 128 PGTPYNQQIANCCKGGVMNSWVQDPATAASSFQISVGAAGTTNKTVRVPRNFTLMGPGPG 187

Query: 188 YTCGPAKIVKPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSFYNDTIVN 247
           YTCGPAKIV+PTKF++ D RR TQA+MTWN+TCTYSQFLAQ+TPTCCVSLSSFYN+TIV 
Sbjct: 188 YTCGPAKIVRPTKFVTTDTRRTTQAMMTWNITCTYSQFLAQRTPTCCVSLSSFYNETIVG 247

Query: 248 CPTCTCGCQNNTAGPGSCVDPDTPHLASVVSTPDKSGNI-EPLVQCTSHMCPIRVHWHVK 307
           CPTC CGCQNN    G+C+DPDTPHLASVVS P K G +  PLVQCT HMCPIRVHWHVK
Sbjct: 248 CPTCACGCQNNRTESGACLDPDTPHLASVVSPPTKKGTVLPPLVQCTRHMCPIRVHWHVK 307

Query: 308 LNYKEYWRVKITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDLNDTAML 367
            NYKEYWRVKITITNFNYR+NYT WNLV QHPN DN+TQ+FSFNYKSL+PY  LNDTAML
Sbjct: 308 QNYKEYWRVKITITNFNYRLNYTQWNLVAQHPNLDNITQIFSFNYKSLTPYAGLNDTAML 367

Query: 368 WGFKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMPPPDAYP 427
           WG KFYND L++AGPLGNVQSE+LFRKDQ+TFTF+KGWAFPRR+YFNGDNCVMPPPD+YP
Sbjct: 368 WGVKFYNDFLSEAGPLGNVQSEILFRKDQSTFTFEKGWAFPRRIYFNGDNCVMPPPDSYP 427

Query: 428 YLPNASSRPAISLLMTILISLALLFNF 454
           +LPN  SR   S +  +L+ L + F F
Sbjct: 428 FLPNGGSRSQFSFVAAVLLPLLVFFFF 454

BLAST of Spg022745 vs. TAIR 10
Match: AT3G02210.1 (COBRA-like protein 1 precursor )

HSP 1 Score: 725.7 bits (1872), Expect = 2.3e-209
Identity = 320/434 (73.73%), Postives = 376/434 (86.64%), Query Frame = 0

Query: 18  FSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGYVAVVTMYNFQQYRHIQA 77
           F + ++FL+S    T +EAYD LDP+GNIT+KWD+++WT DGYVA VT+YNFQQYRHIQA
Sbjct: 15  FGISIIFLVSFSGLTPSEAYDPLDPSGNITVKWDIITWTGDGYVATVTVYNFQQYRHIQA 74

Query: 78  PGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKDPTVVDLLPGTPYNQQIA 137
           PGWTLGW+WAK+EVIW M GGQTTEQGDCS+FKG  PHCCKK P+VVDLLPG+PYNQQIA
Sbjct: 75  PGWTLGWSWAKREVIWGMNGGQTTEQGDCSKFKGTIPHCCKKTPSVVDLLPGSPYNQQIA 134

Query: 138 NCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFTLKAPGPGYTCGPAKIVK 197
           NCC+GGV+NSWAQDPA A S+FQ++VG AGT+NKTVR+PKNFTLKAPGPGYTC PAKIVK
Sbjct: 135 NCCRGGVLNSWAQDPATAVSAFQLTVGQAGTTNKTVRVPKNFTLKAPGPGYTCSPAKIVK 194

Query: 198 PTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSFYNDTIVNCPTCTCGCQN 257
           PT+F+  DKRR+TQALMTWNVTCTYSQFLAQKTPTCCVSLSSFYN TIV+CPTC+CGC+ 
Sbjct: 195 PTRFIGTDKRRVTQALMTWNVTCTYSQFLAQKTPTCCVSLSSFYNKTIVSCPTCSCGCR- 254

Query: 258 NTAGPGSCVDPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIRVHWHVKLNYKEYWRVKI 317
           NT+ PG+CVDP  P +ASV+  P K+  I PLVQCT HMCP+R+HWHVK+NYK+YWRVK+
Sbjct: 255 NTSQPGNCVDPKGPRIASVIPNPGKNAYIPPLVQCTKHMCPVRIHWHVKVNYKQYWRVKV 314

Query: 318 TITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDLNDTAMLWGFKFYNDLLN 377
           TITNFNY MNY+ WNLVVQHPNFDN+TQ FSFNYK L+PY  +NDT +LWG KFYNDLL 
Sbjct: 315 TITNFNYNMNYSQWNLVVQHPNFDNLTQTFSFNYKPLTPYASINDTGILWGIKFYNDLLM 374

Query: 378 QAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMPPPDAYPYLPNASSRPAI 437
           QAGP GNVQSELLF+K+ + FTF+KGWAFPRR+YFNGDNCVMPPPD+YP+LPN  S  ++
Sbjct: 375 QAGPFGNVQSELLFQKEASAFTFEKGWAFPRRIYFNGDNCVMPPPDSYPWLPNTGSHKSV 434

Query: 438 SLLMTILISLALLF 452
             L   +  L ++F
Sbjct: 435 GSLFAAMALLLIVF 447

BLAST of Spg022745 vs. TAIR 10
Match: AT3G29810.1 (COBRA-like protein 2 precursor )

HSP 1 Score: 707.6 bits (1825), Expect = 6.5e-204
Identity = 326/439 (74.26%), Postives = 383/439 (87.24%), Query Frame = 0

Query: 18  FSVVLLFLLS--SFSFTSTEAYDALDPNGNITIKWDVMSWTPDGYVAVVTMYNFQQYRHI 77
           FS +LLFL S  SF+FT+TEAYDALDP GNITIKWD+MSWT DGYVAVVT++NFQQYRHI
Sbjct: 8   FSFLLLFLCSWTSFTFTTTEAYDALDPYGNITIKWDIMSWTGDGYVAVVTIFNFQQYRHI 67

Query: 78  QAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKDPTVVDLLPGTPYNQQ 137
           +APGW LGW+W KKEVIWSM+GGQ TEQGDCS+FKGN PHCCKK P +VDLLPGTPYNQQ
Sbjct: 68  EAPGWQLGWSWMKKEVIWSMVGGQATEQGDCSKFKGNIPHCCKKTPAIVDLLPGTPYNQQ 127

Query: 138 IANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFTLKAPGPGYTCGPAKI 197
           I+NCC+GGVI++WAQDPA A SSFQ+SVG +GT+N TVR P+N TLKAPGPGYTCGPAK+
Sbjct: 128 ISNCCRGGVISAWAQDPATAISSFQISVGQSGTTNTTVRAPRNITLKAPGPGYTCGPAKL 187

Query: 198 VKPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSFYNDTIVNCPTCTCGC 257
           VKP++F+S DKRR TQ+L+TWN+TCTYSQFLA+KTPTCCVSLS+FYN+TIV CPTC+CGC
Sbjct: 188 VKPSRFISADKRRKTQSLLTWNITCTYSQFLARKTPTCCVSLSAFYNETIVPCPTCSCGC 247

Query: 258 QNNTAGPGSCVDPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIRVHWHVKLNYKEYWRV 317
           QN++   G+CVD   P +ASVV    K+ N+EPL+QCT HMCPIRVHWHVK +YKEYWRV
Sbjct: 248 QNSSQA-GTCVD---PKIASVVPALGKN-NLEPLLQCTQHMCPIRVHWHVKTSYKEYWRV 307

Query: 318 KITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDLNDTAMLWGFKFYNDL 377
           K+ ITNFNY MNY+ WNLVVQHPNFDN+T+LFSFNYK L+PY ++NDTAMLWG KFYND 
Sbjct: 308 KVAITNFNYNMNYSQWNLVVQHPNFDNLTKLFSFNYKPLNPYLNINDTAMLWGIKFYNDF 367

Query: 378 LNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMPPPDAYPYLPNAS--- 437
           L+QAGP+GNVQSELLF+K+   FTF+KGWAFPRR+YFNGDNCVMPPPD+YP+LPNAS   
Sbjct: 368 LSQAGPVGNVQSELLFQKNPLEFTFEKGWAFPRRIYFNGDNCVMPPPDSYPWLPNASPNI 427

Query: 438 -SRPAISLLMTILISLALL 451
            + P + LL+T L  L L+
Sbjct: 428 ATSPFVILLITFLSVLILM 441

BLAST of Spg022745 vs. TAIR 10
Match: AT5G15630.1 (COBRA-like extracellular glycosyl-phosphatidyl inositol-anchored protein family )

HSP 1 Score: 649.0 bits (1673), Expect = 2.7e-186
Identity = 301/431 (69.84%), Postives = 345/431 (80.05%), Query Frame = 0

Query: 18  FSVVLLFLLSSFSFTSTEAYDALDPNGNITIKWDVMSWTPDGYVAVVTMYNFQQYRHIQA 77
           FS    F +  F+ T   AYD LDP+GNITIKWD+MSWT DGYVA VTM NFQ YRHIQ 
Sbjct: 5   FSFCFFFFMIIFTAT---AYDPLDPSGNITIKWDIMSWTADGYVATVTMNNFQIYRHIQN 64

Query: 78  PGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRFKGNTPHCCKKDPTVVDLLPGTPYNQQIA 137
           PGWTLGWTWAKKEVIWSM+G QTTEQGDCS+FKGN PHCCKK PTVVDLLPG PYNQQ +
Sbjct: 65  PGWTLGWTWAKKEVIWSMVGAQTTEQGDCSKFKGNVPHCCKKTPTVVDLLPGVPYNQQFS 124

Query: 138 NCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFTLKAPGPGYTCGPAKIVK 197
           NCCKGGVI +W QDP+ A S FQVS G AGT+NKTV+LPKNFTL  PGPGYTCGPAKIV 
Sbjct: 125 NCCKGGVIGAWGQDPSAAVSQFQVSAGLAGTTNKTVKLPKNFTLLGPGPGYTCGPAKIVP 184

Query: 198 PTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSFYNDTIVNCPTCTCGCQN 257
            T F++ DKRR TQALMTWNVTCTYSQFLA+K P+CCVS SSFYNDTI  CP+C CGC+N
Sbjct: 185 STVFLTTDKRRKTQALMTWNVTCTYSQFLARKHPSCCVSFSSFYNDTITPCPSCACGCEN 244

Query: 258 NTAGPGSCVDPDTPHLASV-VSTPDKSGNIEPLVQCTSHMCPIRVHWHVKLNYKEYWRVK 317
                 SCV  D+  L    ++TP K     PL+QCT HMCP+RVHWHVK NYK+YWRVK
Sbjct: 245 KK----SCVKADSKILTKKGLNTPKKDNT--PLLQCTHHMCPVRVHWHVKTNYKDYWRVK 304

Query: 318 ITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPYGDLNDTAMLWGFKFYNDLL 377
           I ITNFNYRMN+TLW L +QHPN +NVTQ+FSF+YK +SPYG +NDT M +G KFYNDLL
Sbjct: 305 IAITNFNYRMNHTLWTLAIQHPNLNNVTQVFSFDYKPVSPYGSINDTGMFYGTKFYNDLL 364

Query: 378 NQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMPPPDAYPYLPNASSRPA 437
            +AGP GNVQSE+L +KDQ TFTF +GWAFPR+VYFNGD C++PPPD+YP+LPN++    
Sbjct: 365 MEAGPSGNVQSEVLLQKDQKTFTFKQGWAFPRKVYFNGDECMLPPPDSYPFLPNSAQGNF 424

Query: 438 ISLLMTILISL 448
            S  +TIL+ L
Sbjct: 425 ASFSLTILLLL 426

BLAST of Spg022745 vs. TAIR 10
Match: AT1G09790.1 (COBRA-like protein 6 precursor )

HSP 1 Score: 465.3 bits (1196), Expect = 5.6e-131
Identity = 227/447 (50.78%), Postives = 294/447 (65.77%), Query Frame = 0

Query: 19  SVVLLFLLSSFSF------TSTEAYDALDPNGNITIKWDVMSWTPDGYVAVVTMYNFQQY 78
           +V+L  +LS   F         + YD LDP G I IKWD++  +P  +   VT+ N Q+Y
Sbjct: 12  TVILCSILSPTRFMIMIDKMVADGYDPLDPFGKIIIKWDLLLSSPGQHHVQVTLENMQEY 71

Query: 79  RHIQAPGWTLGWTWAKKEVIWSMMGGQTTEQGDCSRF--KGNTPHCCKKDPTVVDLLPGT 138
           RH++ PGW L W W  +EVIW M G +TTEQG+CS F   GN PHCC + PT+VDLLPG 
Sbjct: 72  RHVEKPGWKLSWHWLNQEVIWDMKGAETTEQGNCSAFASSGNLPHCCLERPTIVDLLPGA 131

Query: 139 PYNQQIANCCKGGVINSWAQDPANAASSFQVSVGAAGTSNKTVRLPKNFTLKAPGPGYTC 198
             N Q+ANCC+GGV+ S +QD AN  S+F ++VG++    +   +P NF +    PGY+C
Sbjct: 132 SLNVQVANCCRGGVLTSMSQDHANHVSAFHMTVGSSPDGPEEFNMPSNFDIGV--PGYSC 191

Query: 199 GPAKIVKPTKFMSGDKRRITQALMTWNVTCTYSQFLAQKTPTCCVSLSSFYNDTIVNCPT 258
             A  V PTKF +   RR TQAL TW   C YSQF +  +P CCVSLS+FY   IV CPT
Sbjct: 192 DNATSVSPTKFSTDKGRRKTQALATWEAVCVYSQFRSSPSPKCCVSLSAFYYQNIVPCPT 251

Query: 259 CTCGCQNNTAGPGSCV-DPDTPHLASVVSTPDKSGNIEPLVQCTSHMCPIRVHWHVKLNY 318
           C+CGC ++      CV D + P        PD+   + P+V+C+ HMCPIR+HWHVK+NY
Sbjct: 252 CSCGCSSS-----HCVKDGELPPYLEQKHDPDE--EVSPVVKCSDHMCPIRIHWHVKVNY 311

Query: 319 KEYWRVKITITNFNYRMNYTLWNLVVQHPNFDNVTQLFSFNYKSLSPY-GDLNDTAMLWG 378
           +EYWRVKIT TNFN   NYT WNLVV HPN  +V Q+FSFNYKSL+PY   +NDT M WG
Sbjct: 312 REYWRVKITATNFNTMKNYTNWNLVVLHPNLKSVQQVFSFNYKSLTPYQNSINDTGMFWG 371

Query: 379 FKFYNDLLNQAGPLGNVQSELLFRKDQNTFTFDKGWAFPRRVYFNGDNCVMPPPDAYPYL 438
            +FYND+L Q G +GNVQ+ELL +KD   FTF +GWAFPRR+ FNGD CVMP PD +P L
Sbjct: 372 VQFYNDVLLQEGKIGNVQTELLLKKDMGNFTFREGWAFPRRILFNGDECVMPSPDDFPRL 431

Query: 439 PNASSRPAISLLMTILISLALLFNFEL 456
           P ++   + S    ++ S++++F F L
Sbjct: 432 PKSAHSSSSS--SAVISSVSVVFCFLL 447

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878869.15.2e-26495.36protein COBRA-like [Benincasa hispida][more]
XP_022933389.12.6e-26394.51protein COBRA-like [Cucurbita moschata] >KAG6587595.1 Protein COBRA, partial [Cu... [more]
KAG7021561.12.6e-26394.51Protein COBRA, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023531135.15.8e-26394.29protein COBRA-like [Cucurbita pepo subsp. pepo][more]
XP_022966079.12.9e-26294.29protein COBRA-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q94KT87.2e-22478.52Protein COBRA OS=Arabidopsis thaliana OX=3702 GN=COB PE=2 SV=1[more]
Q60E701.8e-21178.13COBRA-like protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=BC1L4 PE=2 SV=1[more]
Q9SRT73.2e-20873.73COBRA-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=COBL1 PE=2 SV=1[more]
Q6Z4G81.2e-20776.61COBRA-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BC1L6 PE=2 SV=1[more]
Q8L8Q79.1e-20374.26COBRA-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=COBL2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1EZM81.3e-26394.51COBRA-like protein OS=Cucurbita moschata OX=3662 GN=LOC111440818 PE=3 SV=1[more]
A0A6J1HSM91.4e-26294.29COBRA-like protein OS=Cucurbita maxima OX=3661 GN=LOC111465789 PE=3 SV=1[more]
A0A5D3BHU57.6e-26194.04COBRA-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold346G0... [more]
A0A1S3BVW77.6e-26194.04COBRA-like protein OS=Cucumis melo OX=3656 GN=LOC103494060 PE=3 SV=1[more]
A0A6J1E1U21.7e-26093.60COBRA-like protein OS=Cucurbita moschata OX=3662 GN=LOC111430062 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G60920.15.1e-22578.52COBRA-like extracellular glycosyl-phosphatidyl inositol-anchored protein family [more]
AT3G02210.12.3e-20973.73COBRA-like protein 1 precursor [more]
AT3G29810.16.5e-20474.26COBRA-like protein 2 precursor [more]
AT5G15630.12.7e-18669.84COBRA-like extracellular glycosyl-phosphatidyl inositol-anchored protein family [more]
AT1G09790.15.6e-13150.78COBRA-like protein 6 precursor [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006918COBRA, plantPFAMPF04833COBRAcoord: 60..223
e-value: 6.1E-74
score: 247.4
IPR006918COBRA, plantPIRSFPIRSF038122COBRAcoord: 9..455
e-value: 4.2E-255
score: 844.0
IPR006918COBRA, plantPANTHERPTHR31673PROTEIN COBRAcoord: 10..452
NoneNo IPR availablePANTHERPTHR31673:SF36COBRA-LIKE PROTEINcoord: 10..452

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg022745.1Spg022745.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010215 cellulose microfibril organization
biological_process GO:0052324 plant-type cell wall cellulose biosynthetic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0031225 anchored component of membrane