Spg022318 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg022318
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionMethylthioribose-1-phosphate isomerase
Locationscaffold2: 9147750 .. 9154557 (-)
RNA-Seq ExpressionSpg022318
SyntenySpg022318
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAATTTGTTGTTTATCAAAAAAAAAAAAAAAAAATGAAAGAGAACAAAATTTAAAATCAAATTCAACTCTTCATTGTTTTCAAAAGACTACAACTTTGACACCAATTCGACCTACGAGTGGACAAAAACATTATCTTTTTCCTCAAAAAAAAAAAAAAAAAAAACATTGTCTTTATTTTCTAACATTACTGCCAACGAAGCACGATTATCAAAGTTTTAATCTTACATTATTTTTCCTGTTCAAACTTCAAAGGGCGTCAGCGATTCTGCTCGACTGCAGTCACATTTTTAATTCACCCCTTCTAGACAAATTCGACCCCGTGATTCTTATTCTAATCTTTCCCCTAATCCAATGGAGAAAATTTATTCTTTTTTCTCTCTGAAATGTAATGGAGAAATTTGAATTTTGCTTTTGAAAGTCTCCATTATCACTGGAAAGTACCAGGAGCATTCATTCGCCGCCTCTGTTCTGTCATCTTCAGGTCAGCCCCTTCCCCTTTATTTTCGATGTCCGCTCAATTCTGAAGCAGCCCATCAGAGGAATTGAATTCCCCTTTTGAATCGTTTTGTTGTTTGGGGTTTCTTCTTCGCTTCTGCAGATTTCGCCGGTTTAATTTCTTGTAAGCATAATGGCTTGGGATTCGAGCAATGGCGGTGAAGTCGGAGCGGATAACTCTCTCCAGTCCATCTGTTACAAGCGCGGGTCCTTGCGGCTGCTCGATCAGGTAGTGTTTGGTTTTTTGGCTTTTGGGTTCTTGGTAGAAATTGAAATGATGCCTGTTGATGATGGAACTTTTCTCAGCTGATCGAACTGAAACGATGTTTGTTTTGCTTGTTCTCTGCTATTTAAGTATTAAGTGACAAAGAAACTTGAGTACTTCATTGATGTACTGTATGGACCACTATGCATGTTTTTGTTTCGTTCATGCTTTCTGTTCAATACTTTCCTTCAAGCATTGAACTATGTTTGTTTATTGACTGGTCCACACATGAAGACGCTTTAATTGACAGTGGTTGCTTTTGAACAGAGGAAGCTCCCTCTGGAGACTGTTTACCTGGACATCAAGGATGCAACAGATGGATGGTAATTTATTGCAATCTTTGTTTTGTCTAATCTGTTTCATGGTGTTTCTTCCTAACCCTCATGCTCTTTTGAACCCTTTTTGTTTTCACTTTTTGAAGATCGTGTTCTTTTGAATTTTGAAGATTTGTTGAATACCATTGCTTGGATATGATTTGCTAAACCCGAACCTCCATTAGCTGGTCTGTGGTTTAGAGGGTTTGTGTTGGACTTGTGTGTTTTTGGGATCTTGGGTTCAACTCCTATTTTATGGATTTCTGAGAACTTTTTAACTGTAGGATCAAATGATGGACTCGTAGATTACAAATGTTGAGCTCCTAGTGTGTCTTTATGATATGAGGCATCTGATTGTTAAAACCATTGTATCTAGTAAATTTTTGTTATACATTTCACCCTATGATGACTGTAACAAAGGATTCGAGATAGTTCCTCAGTACTCTTAGTCTCTTCCAGAAATTACATCCTTTCTGTTTATAAAACCAATGTCTTCTCATGAAATTACCCATCACTGCCTAAATATCTGACTTGTTGAATGCGAAGCATTTTCAAGCTCCGGTTGTTTTCAGCGAAGTTTGTGATATTCTAAAAAATCTACTTGTATGTCAGGCATGCAATAAGGGATATGGTTGTACGTGGTGCACCGGCCATTGCTATTGCAGCAGCCCTTGCTTTGGCAGCAGAAGTGTACAACTCGAATGCTTTTAATGGGACGTCCAATGATGCAGCTTCCTTCATTGCAGAGAAACTGGACTACTTAGTCTCCAGGTTGCCTATGCTGTGAATATAATTTTCTTCATTATTTATCAGTGTTTTTGCACAGGAATTTTTTATTGGCGATGTTGTTAAAATGTTTTTGTTCATTCCCATTAGCTTTTAGACCATAGCTCATGTGTTTTTTCCTCATTTGTAGCCGACCAACTGCTGTTAATCTTGGCGATGCTGCTATTAAACTTAAAGAAATTGTCTCTGAGGCTGCTGCTATTTCTTCTGAAGCCAAGAGTGTTGTCCAAGTATGAGTTTGGAGTTCCATTTGGCTGTTCAGTTTGAGTTGTTAATCTTTAGTTTACTTGTATTTCAGCATTACAAGTGCTATATTTCTTATATGCATTGTTAAAAACACTTTAGTTCTTTCATCTTTTATCAAATCTGTTTGAGGTCATGCTGAAATATGAAGGATGTCTCATTTGAAAATCCTTTTGAGTATTTATTACTTGTTGAATAATGAATAGGCGTATATAGAGGCTGCCGAAGCAATGCTGGAGGATGACGTTGCTTCAAATAAAGCCATTGGGTCCTATGGAGCAACTTATATCCGTGATCAACAAAAATCCTTGGAGAAGTTTTCCGTTCTAACGCATTGCAATACTGGCAGGTATCTTCTTAAACGTATATGCTAGCCTCTTCCCACTCCTTTGCTAATGTATTTCCTTCACTTTTTATTGTGTTCCTTTAACTTCTAATTTACAAAAATCCGACAACTGGGCGCTTGCATGGCCGTTTCACCCTTGGAACATAAATGCCTCCTGACTTACTATTCTTGATCTTCTTCACGTTTCAGTCTAGCAACGGCTGGATATGGTACTGCCCTTGGTGTAATTCGTGCACTTCACTCTGCTGGTGTGTTAGAAAGGGCATACTGTACCGAAACACGGCCTTTTAATCAAGTGAGTTTCCTTTATGAATTCTTTGATTTTCAATTTCCTCTCTTTATGATAGAACTATCAACATAATTTATGAAAAAACCTTGCTCAATTGTAAAGTAAGAACGTCTGAGTGTCTATATCAGCTCTAGTTAACTTTTCTATCAATATCAGTGGTGTTTGTATTAAGTAGATGTTTTCTTTTCAAACTTGTTATGCTCATTTTCCTGATTTTGATATCTTCAACTGTGTATATTACACCGTTTTGTGGACTAGGCATACGTCTCTCAGCACGTTGTTTATTAACTTAACTTTTGCCAATTATTGTAGGGATCCCGACTCACGGCCTATGAGTTGGTCCATGAAAAGATACCTTCTACTCTGATAGCAGATTCTGCTGCCGCCGCCTTGATGAAATTTGGAAGGGTGAATGCTGTTGTAGTTGGGGCTGATCGCGTAGCTGCAAATGGTATTTTGATGTTCATTTATATACGGATTGCGGTATCGGTTTGACAAGGAAGAGTGTTTGAGAGTTCTATTTAATGAATTGCAGGTGATACTGCAAATAAGATTGGAACCTATAGTCTTGCATTGTGTGCAATGCATCACAAGATTCCGTTCTTTGTGGCTGCACCATTGACTTCCATAGACTTATCCCTTTCTTCTGGAGAAGAAATTGTCATCGAGGAAAGGTCTCCCAAAGAGTTGTTGAACTCACGTGGAGGTCTCGGCGAGCAAGTCGCTGCATCTGGCATCGCTGTCTGGAACCCTGCTTTCGATGTTACTCCTGCTTATCTCATCGCAGGGATCATAACAGAAAAGGTGATCCTCTCTACCTGTTTCCTGTTTAGAAAAGTCGAAAGCATCCAACTTCCATTGACATGTTTTATCTTTGCAGGGAGTCATCACGAAGGCAGGTTCTGAATCTTTCAATATAAAGGAGTTCGTTCAAAAGACGGCAGGGACTCCTGCATAGAGAGGCGGCCTCGTGGACGACATGCTATTCTGCAGGTAAACTAGAAATAAAAATGAACTAGATATCTTCAAATTTGATGCAAACTGTGTAGGTCGAGTTGTCGATGAAATAAACGTTCTTGTTCATCAAATCAATAGATACAGGAATGCCATTTATCAAATCTCTGTCAGTTCATGGCTCTCTTTCTGAAGGTATCAGAGGAATCGATATATATACAACTTGATGATATTCTGGCTTTGTTTCCATTGTCCAATGAATGATGAAAAATGTTTACAGTTTTCTTATTTCTTTACATAACTAAAGAAAAATTTTGGAGTCGCCAACACGAGCATAGCTCAACTGATATAAAGCTTGTAATAATGATCAAGAGGCCTGCGGTTCAAATCTCTTCACATAAAAGAAAGAAAAATTTTGGAGTAACTTGCCAATCGCAATTCTTCTTGTGCATGCATTTTAAAACTGGGAAACTGATAAATCATGAACTGGTAAAATCACATAAGAAAACAAACTTGGAGAGAAAGGTTTGCACCTTTCACTCATAATCATATATAGCTGATAATTTTGGTCAGCACTGCAGAAAACTGTTGCTGGAGCCCCAATCAACACTGTAAAAGAATGGCAACCTCGTTTCAGGGGTACAAGTCGAAATCAAAAAAGCCAACGAAACCAACCAAGCTGACTCGAACTATACTTGTCAGTCAATTCAGCTCTTGAACGATAAACATTTGATCACATTCGTCAAGTTTGGTTCGGTTTTGTCGGTTTCAATCGTATCAGTTCAGACACTTATAAAGACTGCTCGAAATCGATCTAAGGCCAACAGAACTACAAGAAAACCACCACTGAAACTTGTACTTGAGTGGGCTCTATCCCAGCAAGACCTGACCTGGCCTCATTTCCCTTTCCAATATCAGTTGATCCAACACTTTCCATGTTCCTTTTTTCCTCTTCCTTTCTCTTTGCTGTGTAGTTTTCAATGAAGAGACAGAAGAAAAAGATCAGATCATGCTTAGCCTACAAGGCAAAAAGGGAAAATATCTTTGGTCTTAATCTTAATGACCAAACAAAGGGCAAAAGAAAACAAAAATGGGGGTGGTGCAGAACAGAGCCTTAGAGATGTACATGGCTGTCTGTCTCAAACACAAACACTTGAAAAGAAAAAAACAGACCGCCATGCACATCCATTTTGTAAAAAGATTCCTTTTTCTCAAAACATAGGAAGAAACGACATGTGGCCCCCGACCGACGAGGAAGTCGACAGACACAGACAGCAGCTCCCAATCGGATGCTATGGCTCTGTTGTTGTTGCGTGTCGCATCAGAAGAAAGTGGCATGGCTTTGCACATGGCGATATGGCTTCTCTGTTTCTTTAAAATCCCATGTCCTTTTCTGATACCCAATTTCAATCTTCTTCATCTTCACCTCCACCAACAACTTTTTCCTCTGGTTTTTCTGACTCGTTTCAGATCCTTTCTGCGACCGGTTTTCAGAGACAAAACCAAAACAAAACCAAACATTGTTTAATGATAATAATAACATAACAGTCTTGTGTCTGAAAACGTTTCGGGAATCCAAACGTTGTTGGGAACTTCAATGGCTAACAAGTATAAATAAAACTCACGAGCTTAGAAACAGAGTCATCTGATCGGATCAGAAGCAGAACAAGAAGAAAGCCATTGCTTTTACAAATGGGGTTGCTTTGTTTTATATTATTTTTCAAATGCGTTACCAAAAGAAGACGAAGATTCTCACCCAATGACCTTAAAAACTCCGAGTTTTTTTTTTTTTTTTTCTTCTTTTTCTTTTTCTCAACTACTTGGGGGGAAATGTAATTACCCACTATTTTGACTGGTTTAATGGCAATAAATCTATTGCTTAAACCCTTATTATAGCAATGCATTTACTACAAATGGGAAAGTGCCAACAGCCCAATTGTCAATAAATGATAAATCCTTTTGGTTGTCCTCTGGTTTTCTCTCCCTCTTCCAAAACAGAACAGAGAGGCCAAAGAACAGAGGAAGCAGATAAGAGTGAGTTGTCATGCTGCTGGCGATGGCGTTACCAAACTCCGACTCTCAGGCGGCGACGGGGAAGTCTACGGCGGCGGCTACGGCGGAGGTCGAGTGTGTGAAATGCTACTCCTGCGGATTTACAGAGGATTGCACCCCTGCTTACATTTCTCGAGTTCGTGATCGCTTCCATGGCCGGTGGATCTGTGGACTCTGCATCGAGGCGGTGAAAGACGAAGTTGTGAGATCAGGAACGCTGATTTCCACCGAAGAAGCGCTGGTTCGACACGCGAGTTTCTGCAAGGAGTTCAGATCGACGAACCCTCTGGACGAAACAGAGCACCCCATCTCCGCCATGGGGCGGCTAATGCGGCGGAGCTTGGATTCTCCCCGAGTGCACCGCTCGAATTCCAGCAGCGTAATCGAACTCGAACCGATCATCGACATCGGCGATTCGGCCAGGCTCCAACGATCCGGGAGTTGCTTTCCTTCCTTGTCTAGTTAAATTAAATAAAAAAAACAGAGTAACATTTTCAAGCGACTAACTTGCTTCAGTGGGTGGGTTCGATTAGACTTCTCCGACGATTTTCAAGCCATTTAACGAAATGGGTTTGGGAATTCGAGGGGAAAACTGACCATTTTCTAGCGTTCTGGTTCGTAAATTTCGTCTTTAGTTCTTGTATATCGGATTTACGGATTAAGATGTAATGTAAGTGGAAATTTGTAGCGAAAAAACAGAGCATATTTGTGTTTGCTTGAAAGTTGAAAGTGAAATGAATCCAAGTGTAGATTGAAAATTGAATGGAAAATTTGGGAAGATTGAAGAATCTTGCAGTGGCGGTCGTCTGAGCTCTGCGCTGTTACCATTGTCGGAGAGATAAAGTTCTGTTTCTGGTAATTCAGTAATGGAAGACGCTGGCAATGGTTTGCTTTCTTGTCTGTTTTTGCAAGAGAGTCAGATGTGACAGCTGGTATAGAATTGATAATGATTAATATAAGTTGTTAATCTATAAGTTTTTTCATGTAAAAAAGGTTTTAATTAATTATATTTCTAATCTAAACGTAGTTCATTCGATTAAGATATTTGGATATTAGAAAGGG

mRNA sequence

ATGGCTTGGGATTCGAGCAATGGCGGTGAAGTCGGAGCGGATAACTCTCTCCAGTCCATCTGTTACAAGCGCGGGTCCTTGCGGCTGCTCGATCAGAGGAAGCTCCCTCTGGAGACTGTTTACCTGGACATCAAGGATGCAACAGATGGATGGCATGCAATAAGGGATATGGTTGTACGTGGTGCACCGGCCATTGCTATTGCAGCAGCCCTTGCTTTGGCAGCAGAAGTGTACAACTCGAATGCTTTTAATGGGACGTCCAATGATGCAGCTTCCTTCATTGCAGAGAAACTGGACTACTTAGTCTCCAGCCGACCAACTGCTGTTAATCTTGGCGATGCTGCTATTAAACTTAAAGAAATTGTCTCTGAGGCTGCTGCTATTTCTTCTGAAGCCAAGAGTGTTGTCCAAGCGTATATAGAGGCTGCCGAAGCAATGCTGGAGGATGACGTTGCTTCAAATAAAGCCATTGGGTCCTATGGAGCAACTTATATCCGTGATCAACAAAAATCCTTGGAGAAGTTTTCCGTTCTAACGCATTGCAATACTGGCAGTCTAGCAACGGCTGGATATGGTACTGCCCTTGGTGTAATTCGTGCACTTCACTCTGCTGGTGTGTTAGAAAGGGCATACTGTACCGAAACACGGCCTTTTAATCAAGGATCCCGACTCACGGCCTATGAGTTGGTCCATGAAAAGATACCTTCTACTCTGATAGCAGATTCTGCTGCCGCCGCCTTGATGAAATTTGGAAGGGTGAATGCTGTTGTAGTTGGGGCTGATCGCGTAGCTGCAAATGGTGATACTGCAAATAAGATTGGAACCTATAGTCTTGCATTGTGTGCAATGCATCACAAGATTCCGTTCTTTGTGGCTGCACCATTGACTTCCATAGACTTATCCCTTTCTTCTGGAGAAGAAATTGTCATCGAGGAAAGGTCTCCCAAAGAGTTGTTGAACTCACGTGGAGGTCTCGGCGAGCAAGTCGCTGCATCTGGCATCGCTGTCTGGAACCCTGCTTTCGATGTTACTCCTGCTTATCTCATCGCAGGGATCATAACAGAAAAGGGAGTCATCACGAAGGCAGGTTCTGAATCTTTCAATATAAAGGAGTTCGTTCAAAAGACGGCAGGGACTCCTGCATAG

Coding sequence (CDS)

ATGGCTTGGGATTCGAGCAATGGCGGTGAAGTCGGAGCGGATAACTCTCTCCAGTCCATCTGTTACAAGCGCGGGTCCTTGCGGCTGCTCGATCAGAGGAAGCTCCCTCTGGAGACTGTTTACCTGGACATCAAGGATGCAACAGATGGATGGCATGCAATAAGGGATATGGTTGTACGTGGTGCACCGGCCATTGCTATTGCAGCAGCCCTTGCTTTGGCAGCAGAAGTGTACAACTCGAATGCTTTTAATGGGACGTCCAATGATGCAGCTTCCTTCATTGCAGAGAAACTGGACTACTTAGTCTCCAGCCGACCAACTGCTGTTAATCTTGGCGATGCTGCTATTAAACTTAAAGAAATTGTCTCTGAGGCTGCTGCTATTTCTTCTGAAGCCAAGAGTGTTGTCCAAGCGTATATAGAGGCTGCCGAAGCAATGCTGGAGGATGACGTTGCTTCAAATAAAGCCATTGGGTCCTATGGAGCAACTTATATCCGTGATCAACAAAAATCCTTGGAGAAGTTTTCCGTTCTAACGCATTGCAATACTGGCAGTCTAGCAACGGCTGGATATGGTACTGCCCTTGGTGTAATTCGTGCACTTCACTCTGCTGGTGTGTTAGAAAGGGCATACTGTACCGAAACACGGCCTTTTAATCAAGGATCCCGACTCACGGCCTATGAGTTGGTCCATGAAAAGATACCTTCTACTCTGATAGCAGATTCTGCTGCCGCCGCCTTGATGAAATTTGGAAGGGTGAATGCTGTTGTAGTTGGGGCTGATCGCGTAGCTGCAAATGGTGATACTGCAAATAAGATTGGAACCTATAGTCTTGCATTGTGTGCAATGCATCACAAGATTCCGTTCTTTGTGGCTGCACCATTGACTTCCATAGACTTATCCCTTTCTTCTGGAGAAGAAATTGTCATCGAGGAAAGGTCTCCCAAAGAGTTGTTGAACTCACGTGGAGGTCTCGGCGAGCAAGTCGCTGCATCTGGCATCGCTGTCTGGAACCCTGCTTTCGATGTTACTCCTGCTTATCTCATCGCAGGGATCATAACAGAAAAGGGAGTCATCACGAAGGCAGGTTCTGAATCTTTCAATATAAAGGAGTTCGTTCAAAAGACGGCAGGGACTCCTGCATAG

Protein sequence

MAWDSSNGGEVGADNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVRGAPAIAIAAALALAAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKEIVSEAAAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLTHCNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIADSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDLSLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEKGVITKAGSESFNIKEFVQKTAGTPA
Homology
BLAST of Spg022318 vs. NCBI nr
Match: XP_008450940.1 (PREDICTED: methylthioribose-1-phosphate isomerase [Cucumis melo] >XP_016900996.1 PREDICTED: methylthioribose-1-phosphate isomerase [Cucumis melo] >KAA0055765.1 methylthioribose-1-phosphate isomerase [Cucumis melo var. makuwa] >TYK10016.1 methylthioribose-1-phosphate isomerase [Cucumis melo var. makuwa])

HSP 1 Score: 681.8 bits (1758), Expect = 3.4e-192
Identity = 360/378 (95.24%), Postives = 367/378 (97.09%), Query Frame = 0

Query: 1   MAWDSSNGGEVGADNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR 60
           MA DS+NGGEVG DNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR
Sbjct: 1   MALDSNNGGEVGVDNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR 60

Query: 61  GAPAIAIAAALALAAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE 120
           GAPAIAI+AALALAAEVYNS  FNGT NDAAS IA+KLDYLVSSRPTAVNLGDAAIKLKE
Sbjct: 61  GAPAIAISAALALAAEVYNSAPFNGTPNDAASLIADKLDYLVSSRPTAVNLGDAAIKLKE 120

Query: 121 IVSEAAAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLTH 180
           IVS AAAISSEA+SV QA+IEAAEAMLEDDVASNKAIG YGA YIRDQQKSLEKFSVLTH
Sbjct: 121 IVSGAAAISSEARSVFQAFIEAAEAMLEDDVASNKAIGFYGANYIRDQQKSLEKFSVLTH 180

Query: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240
           CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA
Sbjct: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240

Query: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300
           DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL
Sbjct: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300

Query: 301 SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEKGVIT 360
           SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPA+LI GIITEKGVIT
Sbjct: 301 SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAHLICGIITEKGVIT 360

Query: 361 KAGSESFNIKEFVQKTAG 379
           KAGS+SFNIKEFVQKTAG
Sbjct: 361 KAGSDSFNIKEFVQKTAG 378

BLAST of Spg022318 vs. NCBI nr
Match: XP_011660028.1 (methylthioribose-1-phosphate isomerase [Cucumis sativus] >KGN66312.1 hypothetical protein Csa_006848 [Cucumis sativus])

HSP 1 Score: 681.0 bits (1756), Expect = 5.8e-192
Identity = 361/381 (94.75%), Postives = 367/381 (96.33%), Query Frame = 0

Query: 1   MAWDSSNGGEVGADNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR 60
           MA DS+NGGEVG DNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR
Sbjct: 1   MALDSNNGGEVGVDNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR 60

Query: 61  GAPAIAIAAALALAAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE 120
           GAPAIAI+AALALAAEV NS  FNGT NDAASFIA+KLDYLVSSRPTAVNLGDAAIKLKE
Sbjct: 61  GAPAIAISAALALAAEVNNSAPFNGTPNDAASFIADKLDYLVSSRPTAVNLGDAAIKLKE 120

Query: 121 IVSEAAAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLTH 180
           IVS AAAISSEA SV QA+IEAAEAMLEDDVASNKAIG YGA YIRDQQKSLEKFSVLTH
Sbjct: 121 IVSRAAAISSEASSVFQAFIEAAEAMLEDDVASNKAIGLYGANYIRDQQKSLEKFSVLTH 180

Query: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240
           CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA
Sbjct: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240

Query: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300
           DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL
Sbjct: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300

Query: 301 SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEKGVIT 360
           SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPA+LI GIITEKGVIT
Sbjct: 301 SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAHLICGIITEKGVIT 360

Query: 361 KAGSESFNIKEFVQKTAGTPA 382
           KAGS+SFNIKEFVQKTAG  A
Sbjct: 361 KAGSDSFNIKEFVQKTAGPSA 381

BLAST of Spg022318 vs. NCBI nr
Match: XP_038880122.1 (methylthioribose-1-phosphate isomerase [Benincasa hispida] >XP_038880123.1 methylthioribose-1-phosphate isomerase [Benincasa hispida])

HSP 1 Score: 678.3 bits (1749), Expect = 3.8e-191
Identity = 363/382 (95.03%), Postives = 369/382 (96.60%), Query Frame = 0

Query: 1   MAWDSSNGGEVGADNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR 60
           MA +S+NGGEVGADNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGW AIRDMVVR
Sbjct: 1   MALESNNGGEVGADNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWQAIRDMVVR 60

Query: 61  GAPAIAIAAALALAAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE 120
           GAPAIAIAAALALAAEVYNS+ FNGT NDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE
Sbjct: 61  GAPAIAIAAALALAAEVYNSDPFNGTPNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE 120

Query: 121 IVSEAAAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLTH 180
           IVS AAAISSEAKSV QAYIEAAEAMLEDDVASNKAIGSYGA YI DQQKSL+KFSVLTH
Sbjct: 121 IVSGAAAISSEAKSVFQAYIEAAEAMLEDDVASNKAIGSYGANYICDQQKSLQKFSVLTH 180

Query: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240
           CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA
Sbjct: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240

Query: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300
           DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL
Sbjct: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300

Query: 301 SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEKGVIT 360
            LSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPA+LI+GIITEKGVIT
Sbjct: 301 YLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAHLISGIITEKGVIT 360

Query: 361 KA-GSESFNIKEFVQKTAGTPA 382
           KA GSESFNIKEFVQKT G  A
Sbjct: 361 KAVGSESFNIKEFVQKTEGARA 382

BLAST of Spg022318 vs. NCBI nr
Match: XP_022147606.1 (methylthioribose-1-phosphate isomerase [Momordica charantia])

HSP 1 Score: 667.2 bits (1720), Expect = 8.7e-188
Identity = 348/381 (91.34%), Postives = 366/381 (96.06%), Query Frame = 0

Query: 1   MAWDSSNGGEVGADNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR 60
           MA DSSNGG+VGADNSLQ+ICY RGSLRLLDQRKLPLETVYLDIK+A DGWHAIRDMVVR
Sbjct: 1   MATDSSNGGDVGADNSLQAICYNRGSLRLLDQRKLPLETVYLDIKNAADGWHAIRDMVVR 60

Query: 61  GAPAIAIAAALALAAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE 120
           GAPAIAIAAALALA EV+NS  FNGTSNDAASFIAEKLDYLVSSRPTAVNL DAAIKLKE
Sbjct: 61  GAPAIAIAAALALAVEVHNSGTFNGTSNDAASFIAEKLDYLVSSRPTAVNLADAAIKLKE 120

Query: 121 IVSEAAAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLTH 180
           IVS A A+SSEA+SV QAYIEA+EAML+DDVASNKAIGSYGATYIR+QQKSLEKFSVLTH
Sbjct: 121 IVSGAVAVSSEARSVYQAYIEASEAMLDDDVASNKAIGSYGATYIREQQKSLEKFSVLTH 180

Query: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240
           CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIP+TLIA
Sbjct: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPATLIA 240

Query: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300
           DSAAAALMKFGRVNA+VVGADRVAANGDTANKIGTYSLALCA HHK+PFFVAAPLTS+DL
Sbjct: 241 DSAAAALMKFGRVNAIVVGADRVAANGDTANKIGTYSLALCAKHHKVPFFVAAPLTSVDL 300

Query: 301 SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEKGVIT 360
           +LSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPA+LI+GIITEKGVIT
Sbjct: 301 TLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAHLISGIITEKGVIT 360

Query: 361 KAGSESFNIKEFVQKTAGTPA 382
           K  SESFNI +FV+KTAGT A
Sbjct: 361 KGDSESFNINDFVKKTAGTCA 381

BLAST of Spg022318 vs. NCBI nr
Match: KAG6588092.1 (Methylthioribose-1-phosphate isomerase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 656.8 bits (1693), Expect = 1.2e-184
Identity = 351/382 (91.88%), Postives = 360/382 (94.24%), Query Frame = 0

Query: 1   MAWDSSNGGEVGADNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR 60
           MA D +NGGE+GA+NSLQSICYKRGSLRLLDQRKLPLET YLDIKD+ DGWHAIRDMVVR
Sbjct: 1   MACDPNNGGEIGAENSLQSICYKRGSLRLLDQRKLPLETRYLDIKDSKDGWHAIRDMVVR 60

Query: 61  GAPAIAIAAALALAAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE 120
           GAPAIAIAAALALAAEVYN +AFNG+ NDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE
Sbjct: 61  GAPAIAIAAALALAAEVYNLDAFNGSPNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE 120

Query: 121 IVSEAAAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLTH 180
           IVS A AIS +AKSV QAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSL K SVLTH
Sbjct: 121 IVSRAVAISPDAKSVFQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLAKLSVLTH 180

Query: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240
           CNTGSLATAGYGTALGVIRALHSA VLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA
Sbjct: 181 CNTGSLATAGYGTALGVIRALHSADVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240

Query: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300
           DSAAAALMK GRVNAVVVGADRVAANGDTANKIGTYSLALCAMHH IPFFVAAPLTSIDL
Sbjct: 241 DSAAAALMKSGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHNIPFFVAAPLTSIDL 300

Query: 301 SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEKGVIT 360
           SLSSGEEIVIEERSPKELLN+RGGLGEQVAASGI VWNPAFDVTPA LI+GIITEKGVIT
Sbjct: 301 SLSSGEEIVIEERSPKELLNARGGLGEQVAASGIDVWNPAFDVTPARLISGIITEKGVIT 360

Query: 361 KAGSESFNIKEFVQK-TAGTPA 382
           K GSE FNIKEFVQK  AGT A
Sbjct: 361 KVGSEDFNIKEFVQKIAAGTLA 382

BLAST of Spg022318 vs. ExPASy Swiss-Prot
Match: B9RR88 (Methylthioribose-1-phosphate isomerase OS=Ricinus communis OX=3988 GN=RCOM_0711420 PE=2 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 5.0e-162
Identity = 294/378 (77.78%), Postives = 345/378 (91.27%), Query Frame = 0

Query: 1   MAWDSSNGGEVGADNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR 60
           MA +S++G E+  D++LQSICY RGSL+LLDQRKLPLET +LDI++++DGW AIR+MVVR
Sbjct: 1   MANNSNSGSELEVDDTLQSICYNRGSLKLLDQRKLPLETEFLDIRNSSDGWSAIREMVVR 60

Query: 61  GAPAIAIAAALALAAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE 120
           GAPAIAIAAAL+LA EV+N + F GTSNDAASF+ +KL+YLVSSRPTAVNL DAA KLKE
Sbjct: 61  GAPAIAIAAALSLAVEVFNFDDFTGTSNDAASFLFKKLEYLVSSRPTAVNLSDAANKLKE 120

Query: 121 IVSEAAAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLTH 180
           ++ +AAA +S++ SV QAYIEAAE ML+DD+ASNKAIGS+GA++I++Q K+ ++ S+LTH
Sbjct: 121 VIYKAAATASDSNSVFQAYIEAAEIMLKDDIASNKAIGSHGASFIQNQLKNPQRLSILTH 180

Query: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240
           CNTGSLATAGYGTALGVIRALH+AGVL RAYCTETRPFNQGSRLTA+ELVHEKIP+TL+A
Sbjct: 181 CNTGSLATAGYGTALGVIRALHAAGVLRRAYCTETRPFNQGSRLTAFELVHEKIPATLVA 240

Query: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300
           DSAAAALMK  +V+AV+VGADRVAANGDTANKIGTYSLALCAMHH IPF+VAAPLTS+DL
Sbjct: 241 DSAAAALMKDDQVSAVIVGADRVAANGDTANKIGTYSLALCAMHHNIPFYVAAPLTSVDL 300

Query: 301 SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEKGVIT 360
           SLSSG+ IVIEERSPKELLNSRGGLGEQVAASGI+VWNPAFDVTPA LI+GIITEKGVIT
Sbjct: 301 SLSSGKHIVIEERSPKELLNSRGGLGEQVAASGISVWNPAFDVTPANLISGIITEKGVIT 360

Query: 361 KAGSESFNIKEFVQKTAG 379
           K  S  F+IK FV++TAG
Sbjct: 361 KIDSSDFDIKNFVKRTAG 378

BLAST of Spg022318 vs. ExPASy Swiss-Prot
Match: B9HCR2 (Methylthioribose-1-phosphate isomerase OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_832064 PE=3 SV=2)

HSP 1 Score: 569.3 bits (1466), Expect = 3.2e-161
Identity = 301/375 (80.27%), Postives = 333/375 (88.80%), Query Frame = 0

Query: 1   MAWDSSNGGEVGADNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR 60
           MA + SNG E   DN+LQSICY RGSL+LLDQRKLPLET YLDIKDA+DGW AIR+MVVR
Sbjct: 1   MATNPSNGLE--GDNTLQSICYHRGSLKLLDQRKLPLETTYLDIKDASDGWLAIREMVVR 60

Query: 61  GAPAIAIAAALALAAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE 120
           GAPAIAI+AAL+LA EV N   FNGT  +AASF+A KLDYLVSSRPTAVNL DAA KLKE
Sbjct: 61  GAPAIAISAALSLAVEVSNLENFNGTPVEAASFLAGKLDYLVSSRPTAVNLSDAATKLKE 120

Query: 121 IVSEAAAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLTH 180
           +VS+AAA +S  +SV QAYIEAAE ML DDVASNKAIGSYGA +I++QQK   K SVLTH
Sbjct: 121 VVSKAAAAASNCQSVFQAYIEAAEIMLADDVASNKAIGSYGARFIQNQQKDPTKLSVLTH 180

Query: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240
           CNTGSLATAGYGTALGVIRALH  GVL+RAYCTETRPFNQGSRLTA+ELVHEKIP+TLIA
Sbjct: 181 CNTGSLATAGYGTALGVIRALHGEGVLQRAYCTETRPFNQGSRLTAFELVHEKIPATLIA 240

Query: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300
           DSAAAALMK  +V+AVVVGADRVAANGDTANKIGTYSLALCAMHH IPF+VAAPLTS D 
Sbjct: 241 DSAAAALMKDSKVSAVVVGADRVAANGDTANKIGTYSLALCAMHHNIPFYVAAPLTSFDS 300

Query: 301 SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEKGVIT 360
           SLSSG+EI+IEERSPKE+LN+RGGLGEQVAASGI+VWNPAFDVTPA LI+GIITEKGVIT
Sbjct: 301 SLSSGKEIIIEERSPKEMLNARGGLGEQVAASGISVWNPAFDVTPASLISGIITEKGVIT 360

Query: 361 KAGSESFNIKEFVQK 376
           K G + F+IK+F+ K
Sbjct: 361 KTGMDDFDIKDFINK 373

BLAST of Spg022318 vs. ExPASy Swiss-Prot
Match: Q948X8 (Methylthioribose-1-phosphate isomerase OS=Nicotiana tabacum OX=4097 GN=CIG2 PE=2 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 1.4e-156
Identity = 285/373 (76.41%), Postives = 333/373 (89.28%), Query Frame = 0

Query: 6   SNGGEVGADNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVRGAPAI 65
           +NG E   D +L SICY RGSL+LLDQRKLPLET+YLDI+D  DGW AI++MVVRGAPAI
Sbjct: 2   ANGNE--GDKTLLSICYNRGSLQLLDQRKLPLETIYLDIQDTKDGWDAIKEMVVRGAPAI 61

Query: 66  AIAAALALAAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKEIVSEA 125
           AIAAAL+LA EV N ++F+GTS+DA+ F++ KLDYLVSSRPTAVNL DAA+KLKE++ +A
Sbjct: 62  AIAAALSLAVEVSNLSSFDGTSDDASDFLSNKLDYLVSSRPTAVNLSDAAVKLKEVIKKA 121

Query: 126 AAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLTHCNTGS 185
           A  + +A SV QAYIEAAE MLEDDV SNKAIGSYGA+++++     +K ++LTHCNTGS
Sbjct: 122 ATTAKDANSVFQAYIEAAEVMLEDDVTSNKAIGSYGASFLKEHLTDSQKITILTHCNTGS 181

Query: 186 LATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIADSAAA 245
           LATAGYGTALGVIRAL++ GVLERA+CTETRPFNQGSRLTA+ELVHE IP+TLIADSAAA
Sbjct: 182 LATAGYGTALGVIRALYTDGVLERAFCTETRPFNQGSRLTAFELVHENIPATLIADSAAA 241

Query: 246 ALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDLSLSSG 305
           ALM  GRV AV+VGADRVAANGDTANKIGTY+LA+ AMHH IPF+VAAPLTSIDLSLSSG
Sbjct: 242 ALMNAGRVKAVIVGADRVAANGDTANKIGTYNLAVSAMHHGIPFYVAAPLTSIDLSLSSG 301

Query: 306 EEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEKGVITKAGSE 365
           +EIVIEERSPKELL++RGGLGEQVAASGI+VWNPAFDVTPA LI+GIITEKGVITK G+E
Sbjct: 302 QEIVIEERSPKELLHTRGGLGEQVAASGISVWNPAFDVTPANLISGIITEKGVITKKGAE 361

Query: 366 SFNIKEFVQKTAG 379
           +F+I EFV+KT+G
Sbjct: 362 TFDIAEFVRKTSG 372

BLAST of Spg022318 vs. ExPASy Swiss-Prot
Match: Q9ZUG4 (Methylthioribose-1-phosphate isomerase OS=Arabidopsis thaliana OX=3702 GN=At2g05830 PE=1 SV=2)

HSP 1 Score: 542.7 bits (1397), Expect = 3.2e-153
Identity = 272/365 (74.52%), Postives = 329/365 (90.14%), Query Frame = 0

Query: 14  DNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVRGAPAIAIAAALAL 73
           D +L++ICYK GSL+LLDQRKLPLET+YL+I+DA+DGW AI++MVVRGAPAIAIAAAL+L
Sbjct: 6   DTTLKAICYKPGSLQLLDQRKLPLETIYLEIRDASDGWSAIQEMVVRGAPAIAIAAALSL 65

Query: 74  AAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKEIVSEAAAISSEAK 133
           A EV+N + F+G+++DA +F+  KLDYLVSSRPTAVNL DAA+KLK ++++A A ++EAK
Sbjct: 66  AVEVFNFHGFDGSASDAVAFLENKLDYLVSSRPTAVNLADAALKLKHVIAKALATATEAK 125

Query: 134 SVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLTHCNTGSLATAGYGT 193
           S+ +AYIEA+E MLEDDV SNKAIG++G + +R Q K+ +K SVLTHCNTGSLATAGYGT
Sbjct: 126 SIFKAYIEASEDMLEDDVVSNKAIGNFGLSLLRQQAKNPDKLSVLTHCNTGSLATAGYGT 185

Query: 194 ALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIADSAAAALMKFGRV 253
           ALGVIRALH+ G+LERAYCTETRPFNQGSRLTA+ELVHEKIP+TLIADSAAAALMK GRV
Sbjct: 186 ALGVIRALHTQGILERAYCTETRPFNQGSRLTAFELVHEKIPATLIADSAAAALMKDGRV 245

Query: 254 NAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDLSLSSGEEIVIEER 313
           + V+VGADRVA+NGDTANKIGTYSLALCA HH IPF+VAAPLTS+DLSLSSG+EIVIEER
Sbjct: 246 DGVIVGADRVASNGDTANKIGTYSLALCAKHHGIPFYVAAPLTSVDLSLSSGKEIVIEER 305

Query: 314 SPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEKGVITKAGSESFNIKEFV 373
           SPKEL+++ GGLGE++AA GI+VWNPAFD+TPA LIAGIITEKGVITK G+++F+I  F 
Sbjct: 306 SPKELMHTHGGLGERIAAPGISVWNPAFDMTPAELIAGIITEKGVITKNGNDTFDISSFA 365

Query: 374 QKTAG 379
           +K  G
Sbjct: 366 KKITG 370

BLAST of Spg022318 vs. ExPASy Swiss-Prot
Match: D7TCD0 (Methylthioribose-1-phosphate isomerase OS=Vitis vinifera OX=29760 GN=VIT_11s0016g00830 PE=2 SV=2)

HSP 1 Score: 535.4 bits (1378), Expect = 5.2e-151
Identity = 281/359 (78.27%), Postives = 318/359 (88.58%), Query Frame = 0

Query: 15  NSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVRGAPAIAIAAALALA 74
           +SLQSICYKRGSL+LLDQRKLPLETVYL+IK A DGWHAIRDMVVRGAPAIAIAAAL+LA
Sbjct: 10  DSLQSICYKRGSLQLLDQRKLPLETVYLEIKGADDGWHAIRDMVVRGAPAIAIAAALSLA 69

Query: 75  AEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKEIVSEAAAISSEAKS 134
            EV N   F GTS+DAASF+  KLDYLV+SRPTAVNL DA  KLK ++S AAA + EA S
Sbjct: 70  VEVSNLEDFIGTSDDAASFLNSKLDYLVTSRPTAVNLSDAVTKLKGVISNAAATAFEAMS 129

Query: 135 VVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLTHCNTGSLATAGYGTA 194
           V QA++EAAE ML +DVA+N+AIG YGA++++   K  +  S+LTHCNTGSLATAGYGTA
Sbjct: 130 VFQAFLEAAEDMLREDVAANRAIGLYGASFLQSHIKDSKNLSILTHCNTGSLATAGYGTA 189

Query: 195 LGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIADSAAAALMKFGRVN 254
           LGVIR++H+ G+L RAYCTETRPFNQGSRLTA+ELVH+ IP+TLIADSAAAALM+ GRV+
Sbjct: 190 LGVIRSVHAEGILLRAYCTETRPFNQGSRLTAFELVHDNIPATLIADSAAAALMQAGRVD 249

Query: 255 AVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDLSLSSGEEIVIEERS 314
           AV+VGADRVAANGDTANKIGTYSLAL A HH IPFFVAAPLTSIDL+LSSG+EIVIEERS
Sbjct: 250 AVIVGADRVAANGDTANKIGTYSLALSAKHHHIPFFVAAPLTSIDLTLSSGQEIVIEERS 309

Query: 315 PKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEKGVITKAGSESFNIKEFV 374
           PKELLNSRGGLGEQVAASGI VWNPAFDVTPA LIAGIITEKGVITK G + F+IK+F+
Sbjct: 310 PKELLNSRGGLGEQVAASGICVWNPAFDVTPADLIAGIITEKGVITKTG-DVFDIKDFI 367

BLAST of Spg022318 vs. ExPASy TrEMBL
Match: A0A1S3BPT0 (Methylthioribose-1-phosphate isomerase OS=Cucumis melo OX=3656 GN=LOC103492378 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.7e-192
Identity = 360/378 (95.24%), Postives = 367/378 (97.09%), Query Frame = 0

Query: 1   MAWDSSNGGEVGADNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR 60
           MA DS+NGGEVG DNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR
Sbjct: 1   MALDSNNGGEVGVDNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR 60

Query: 61  GAPAIAIAAALALAAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE 120
           GAPAIAI+AALALAAEVYNS  FNGT NDAAS IA+KLDYLVSSRPTAVNLGDAAIKLKE
Sbjct: 61  GAPAIAISAALALAAEVYNSAPFNGTPNDAASLIADKLDYLVSSRPTAVNLGDAAIKLKE 120

Query: 121 IVSEAAAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLTH 180
           IVS AAAISSEA+SV QA+IEAAEAMLEDDVASNKAIG YGA YIRDQQKSLEKFSVLTH
Sbjct: 121 IVSGAAAISSEARSVFQAFIEAAEAMLEDDVASNKAIGFYGANYIRDQQKSLEKFSVLTH 180

Query: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240
           CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA
Sbjct: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240

Query: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300
           DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL
Sbjct: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300

Query: 301 SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEKGVIT 360
           SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPA+LI GIITEKGVIT
Sbjct: 301 SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAHLICGIITEKGVIT 360

Query: 361 KAGSESFNIKEFVQKTAG 379
           KAGS+SFNIKEFVQKTAG
Sbjct: 361 KAGSDSFNIKEFVQKTAG 378

BLAST of Spg022318 vs. ExPASy TrEMBL
Match: A0A5D3CHZ0 (Methylthioribose-1-phosphate isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001480 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.7e-192
Identity = 360/378 (95.24%), Postives = 367/378 (97.09%), Query Frame = 0

Query: 1   MAWDSSNGGEVGADNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR 60
           MA DS+NGGEVG DNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR
Sbjct: 1   MALDSNNGGEVGVDNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR 60

Query: 61  GAPAIAIAAALALAAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE 120
           GAPAIAI+AALALAAEVYNS  FNGT NDAAS IA+KLDYLVSSRPTAVNLGDAAIKLKE
Sbjct: 61  GAPAIAISAALALAAEVYNSAPFNGTPNDAASLIADKLDYLVSSRPTAVNLGDAAIKLKE 120

Query: 121 IVSEAAAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLTH 180
           IVS AAAISSEA+SV QA+IEAAEAMLEDDVASNKAIG YGA YIRDQQKSLEKFSVLTH
Sbjct: 121 IVSGAAAISSEARSVFQAFIEAAEAMLEDDVASNKAIGFYGANYIRDQQKSLEKFSVLTH 180

Query: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240
           CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA
Sbjct: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240

Query: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300
           DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL
Sbjct: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300

Query: 301 SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEKGVIT 360
           SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPA+LI GIITEKGVIT
Sbjct: 301 SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAHLICGIITEKGVIT 360

Query: 361 KAGSESFNIKEFVQKTAG 379
           KAGS+SFNIKEFVQKTAG
Sbjct: 361 KAGSDSFNIKEFVQKTAG 378

BLAST of Spg022318 vs. ExPASy TrEMBL
Match: A0A0A0LZL4 (Methylthioribose-1-phosphate isomerase OS=Cucumis sativus OX=3659 GN=Csa_1G597100 PE=3 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 2.8e-192
Identity = 361/381 (94.75%), Postives = 367/381 (96.33%), Query Frame = 0

Query: 1   MAWDSSNGGEVGADNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR 60
           MA DS+NGGEVG DNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR
Sbjct: 1   MALDSNNGGEVGVDNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR 60

Query: 61  GAPAIAIAAALALAAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE 120
           GAPAIAI+AALALAAEV NS  FNGT NDAASFIA+KLDYLVSSRPTAVNLGDAAIKLKE
Sbjct: 61  GAPAIAISAALALAAEVNNSAPFNGTPNDAASFIADKLDYLVSSRPTAVNLGDAAIKLKE 120

Query: 121 IVSEAAAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLTH 180
           IVS AAAISSEA SV QA+IEAAEAMLEDDVASNKAIG YGA YIRDQQKSLEKFSVLTH
Sbjct: 121 IVSRAAAISSEASSVFQAFIEAAEAMLEDDVASNKAIGLYGANYIRDQQKSLEKFSVLTH 180

Query: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240
           CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA
Sbjct: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240

Query: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300
           DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL
Sbjct: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300

Query: 301 SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEKGVIT 360
           SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPA+LI GIITEKGVIT
Sbjct: 301 SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAHLICGIITEKGVIT 360

Query: 361 KAGSESFNIKEFVQKTAGTPA 382
           KAGS+SFNIKEFVQKTAG  A
Sbjct: 361 KAGSDSFNIKEFVQKTAGPSA 381

BLAST of Spg022318 vs. ExPASy TrEMBL
Match: A0A6J1D2V9 (Methylthioribose-1-phosphate isomerase OS=Momordica charantia OX=3673 GN=LOC111016489 PE=3 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 4.2e-188
Identity = 348/381 (91.34%), Postives = 366/381 (96.06%), Query Frame = 0

Query: 1   MAWDSSNGGEVGADNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR 60
           MA DSSNGG+VGADNSLQ+ICY RGSLRLLDQRKLPLETVYLDIK+A DGWHAIRDMVVR
Sbjct: 1   MATDSSNGGDVGADNSLQAICYNRGSLRLLDQRKLPLETVYLDIKNAADGWHAIRDMVVR 60

Query: 61  GAPAIAIAAALALAAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE 120
           GAPAIAIAAALALA EV+NS  FNGTSNDAASFIAEKLDYLVSSRPTAVNL DAAIKLKE
Sbjct: 61  GAPAIAIAAALALAVEVHNSGTFNGTSNDAASFIAEKLDYLVSSRPTAVNLADAAIKLKE 120

Query: 121 IVSEAAAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLTH 180
           IVS A A+SSEA+SV QAYIEA+EAML+DDVASNKAIGSYGATYIR+QQKSLEKFSVLTH
Sbjct: 121 IVSGAVAVSSEARSVYQAYIEASEAMLDDDVASNKAIGSYGATYIREQQKSLEKFSVLTH 180

Query: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240
           CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIP+TLIA
Sbjct: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPATLIA 240

Query: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300
           DSAAAALMKFGRVNA+VVGADRVAANGDTANKIGTYSLALCA HHK+PFFVAAPLTS+DL
Sbjct: 241 DSAAAALMKFGRVNAIVVGADRVAANGDTANKIGTYSLALCAKHHKVPFFVAAPLTSVDL 300

Query: 301 SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEKGVIT 360
           +LSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPA+LI+GIITEKGVIT
Sbjct: 301 TLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAHLISGIITEKGVIT 360

Query: 361 KAGSESFNIKEFVQKTAGTPA 382
           K  SESFNI +FV+KTAGT A
Sbjct: 361 KGDSESFNINDFVKKTAGTCA 381

BLAST of Spg022318 vs. ExPASy TrEMBL
Match: A0A6J1F5M2 (Methylthioribose-1-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111441053 PE=3 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 7.4e-185
Identity = 349/382 (91.36%), Postives = 360/382 (94.24%), Query Frame = 0

Query: 1   MAWDSSNGGEVGADNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVR 60
           MA D +NGGE+GA+NSLQSICYKRGSLRLLDQRKLPLET YLDIKD+ DGWHAIRDMVVR
Sbjct: 1   MACDPNNGGEIGAENSLQSICYKRGSLRLLDQRKLPLETRYLDIKDSKDGWHAIRDMVVR 60

Query: 61  GAPAIAIAAALALAAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE 120
           GAPAIAIAAALALAAEVYN +AFNG+ NDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE
Sbjct: 61  GAPAIAIAAALALAAEVYNLDAFNGSPNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKE 120

Query: 121 IVSEAAAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLTH 180
           IVS A AIS +AKSV QAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSL K SVLTH
Sbjct: 121 IVSRAVAISPDAKSVFQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLAKLSVLTH 180

Query: 181 CNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240
           CNTGSLATAGYGTALGVIRALHSA VLER YCTETRPFNQGSRLTAYELVHEKIPSTLIA
Sbjct: 181 CNTGSLATAGYGTALGVIRALHSADVLERTYCTETRPFNQGSRLTAYELVHEKIPSTLIA 240

Query: 241 DSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDL 300
           DSAAAALMK GRVNAVVVGADRVAANGDTANKIGTYSLALCAMHH IPFFVAAPLTSIDL
Sbjct: 241 DSAAAALMKSGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHNIPFFVAAPLTSIDL 300

Query: 301 SLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEKGVIT 360
           SLSSGEEIVIEERSPKELLN+RGGLGEQVAASGI VWNPAFDVTPA+LI+GIITEKGVIT
Sbjct: 301 SLSSGEEIVIEERSPKELLNARGGLGEQVAASGIDVWNPAFDVTPAHLISGIITEKGVIT 360

Query: 361 KAGSESFNIKEFVQK-TAGTPA 382
           K GSE FN+KEFVQK  AGT A
Sbjct: 361 KVGSEDFNVKEFVQKIAAGTLA 382

BLAST of Spg022318 vs. TAIR 10
Match: AT2G05830.1 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 542.7 bits (1397), Expect = 2.3e-154
Identity = 272/365 (74.52%), Postives = 329/365 (90.14%), Query Frame = 0

Query: 14  DNSLQSICYKRGSLRLLDQRKLPLETVYLDIKDATDGWHAIRDMVVRGAPAIAIAAALAL 73
           D +L++ICYK GSL+LLDQRKLPLET+YL+I+DA+DGW AI++MVVRGAPAIAIAAAL+L
Sbjct: 6   DTTLKAICYKPGSLQLLDQRKLPLETIYLEIRDASDGWSAIQEMVVRGAPAIAIAAALSL 65

Query: 74  AAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAIKLKEIVSEAAAISSEAK 133
           A EV+N + F+G+++DA +F+  KLDYLVSSRPTAVNL DAA+KLK ++++A A ++EAK
Sbjct: 66  AVEVFNFHGFDGSASDAVAFLENKLDYLVSSRPTAVNLADAALKLKHVIAKALATATEAK 125

Query: 134 SVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLTHCNTGSLATAGYGT 193
           S+ +AYIEA+E MLEDDV SNKAIG++G + +R Q K+ +K SVLTHCNTGSLATAGYGT
Sbjct: 126 SIFKAYIEASEDMLEDDVVSNKAIGNFGLSLLRQQAKNPDKLSVLTHCNTGSLATAGYGT 185

Query: 194 ALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLIADSAAAALMKFGRV 253
           ALGVIRALH+ G+LERAYCTETRPFNQGSRLTA+ELVHEKIP+TLIADSAAAALMK GRV
Sbjct: 186 ALGVIRALHTQGILERAYCTETRPFNQGSRLTAFELVHEKIPATLIADSAAAALMKDGRV 245

Query: 254 NAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSIDLSLSSGEEIVIEER 313
           + V+VGADRVA+NGDTANKIGTYSLALCA HH IPF+VAAPLTS+DLSLSSG+EIVIEER
Sbjct: 246 DGVIVGADRVASNGDTANKIGTYSLALCAKHHGIPFYVAAPLTSVDLSLSSGKEIVIEER 305

Query: 314 SPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEKGVITKAGSESFNIKEFV 373
           SPKEL+++ GGLGE++AA GI+VWNPAFD+TPA LIAGIITEKGVITK G+++F+I  F 
Sbjct: 306 SPKELMHTHGGLGERIAAPGISVWNPAFDMTPAELIAGIITEKGVITKNGNDTFDISSFA 365

Query: 374 QKTAG 379
           +K  G
Sbjct: 366 KKITG 370

BLAST of Spg022318 vs. TAIR 10
Match: AT2G05830.4 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 476.1 bits (1224), Expect = 2.7e-134
Identity = 242/323 (74.92%), Postives = 290/323 (89.78%), Query Frame = 0

Query: 56  DMVVRGAPAIAIAAALALAAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAA 115
           +MVVRGAPAIAIAAAL+LA EV+N + F+G+++DA +F+  KLDYLVSSRPTAVNL DAA
Sbjct: 2   EMVVRGAPAIAIAAALSLAVEVFNFHGFDGSASDAVAFLENKLDYLVSSRPTAVNLADAA 61

Query: 116 IKLKEIVSEAAAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKF 175
           +KLK ++++A A ++EAKS+ +AYIEA+E MLEDDV SNKAIG++G + +R Q K+ +K 
Sbjct: 62  LKLKHVIAKALATATEAKSIFKAYIEASEDMLEDDVVSNKAIGNFGLSLLRQQAKNPDKL 121

Query: 176 SVLTHCNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIP 235
           SVLTHCNTGSLATAGYGTALGVIRALH+ G+LERAYCTETRPFNQGSRLTA+ELVHEKIP
Sbjct: 122 SVLTHCNTGSLATAGYGTALGVIRALHTQGILERAYCTETRPFNQGSRLTAFELVHEKIP 181

Query: 236 STLIADSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPL 295
           +TLIADSAAAALMK GRV+ V+VGADRVA+NGDTANKIGTYSLALCA HH IPF+VAAPL
Sbjct: 182 ATLIADSAAAALMKDGRVDGVIVGADRVASNGDTANKIGTYSLALCAKHHGIPFYVAAPL 241

Query: 296 TSIDLSLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITE 355
           TS+DLSLSSG+EIVIEERSPKEL+++ GGLGE++AA GI+VWNPAFD+TPA LIAGIITE
Sbjct: 242 TSVDLSLSSGKEIVIEERSPKELMHTHGGLGERIAAPGISVWNPAFDMTPAELIAGIITE 301

Query: 356 KGVITKAGSESFNIKEFVQKTAG 379
           KGVITK G+++F+I  F +K  G
Sbjct: 302 KGVITKNGNDTFDISSFAKKITG 324

BLAST of Spg022318 vs. TAIR 10
Match: AT2G05830.2 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 475.3 bits (1222), Expect = 4.5e-134
Identity = 242/322 (75.16%), Postives = 289/322 (89.75%), Query Frame = 0

Query: 57  MVVRGAPAIAIAAALALAAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAI 116
           MVVRGAPAIAIAAAL+LA EV+N + F+G+++DA +F+  KLDYLVSSRPTAVNL DAA+
Sbjct: 1   MVVRGAPAIAIAAALSLAVEVFNFHGFDGSASDAVAFLENKLDYLVSSRPTAVNLADAAL 60

Query: 117 KLKEIVSEAAAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFS 176
           KLK ++++A A ++EAKS+ +AYIEA+E MLEDDV SNKAIG++G + +R Q K+ +K S
Sbjct: 61  KLKHVIAKALATATEAKSIFKAYIEASEDMLEDDVVSNKAIGNFGLSLLRQQAKNPDKLS 120

Query: 177 VLTHCNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPS 236
           VLTHCNTGSLATAGYGTALGVIRALH+ G+LERAYCTETRPFNQGSRLTA+ELVHEKIP+
Sbjct: 121 VLTHCNTGSLATAGYGTALGVIRALHTQGILERAYCTETRPFNQGSRLTAFELVHEKIPA 180

Query: 237 TLIADSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLT 296
           TLIADSAAAALMK GRV+ V+VGADRVA+NGDTANKIGTYSLALCA HH IPF+VAAPLT
Sbjct: 181 TLIADSAAAALMKDGRVDGVIVGADRVASNGDTANKIGTYSLALCAKHHGIPFYVAAPLT 240

Query: 297 SIDLSLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEK 356
           S+DLSLSSG+EIVIEERSPKEL+++ GGLGE++AA GI+VWNPAFD+TPA LIAGIITEK
Sbjct: 241 SVDLSLSSGKEIVIEERSPKELMHTHGGLGERIAAPGISVWNPAFDMTPAELIAGIITEK 300

Query: 357 GVITKAGSESFNIKEFVQKTAG 379
           GVITK G+++F+I  F +K  G
Sbjct: 301 GVITKNGNDTFDISSFAKKITG 322

BLAST of Spg022318 vs. TAIR 10
Match: AT2G05830.3 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 475.3 bits (1222), Expect = 4.5e-134
Identity = 242/322 (75.16%), Postives = 289/322 (89.75%), Query Frame = 0

Query: 57  MVVRGAPAIAIAAALALAAEVYNSNAFNGTSNDAASFIAEKLDYLVSSRPTAVNLGDAAI 116
           MVVRGAPAIAIAAAL+LA EV+N + F+G+++DA +F+  KLDYLVSSRPTAVNL DAA+
Sbjct: 1   MVVRGAPAIAIAAALSLAVEVFNFHGFDGSASDAVAFLENKLDYLVSSRPTAVNLADAAL 60

Query: 117 KLKEIVSEAAAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFS 176
           KLK ++++A A ++EAKS+ +AYIEA+E MLEDDV SNKAIG++G + +R Q K+ +K S
Sbjct: 61  KLKHVIAKALATATEAKSIFKAYIEASEDMLEDDVVSNKAIGNFGLSLLRQQAKNPDKLS 120

Query: 177 VLTHCNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPS 236
           VLTHCNTGSLATAGYGTALGVIRALH+ G+LERAYCTETRPFNQGSRLTA+ELVHEKIP+
Sbjct: 121 VLTHCNTGSLATAGYGTALGVIRALHTQGILERAYCTETRPFNQGSRLTAFELVHEKIPA 180

Query: 237 TLIADSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLT 296
           TLIADSAAAALMK GRV+ V+VGADRVA+NGDTANKIGTYSLALCA HH IPF+VAAPLT
Sbjct: 181 TLIADSAAAALMKDGRVDGVIVGADRVASNGDTANKIGTYSLALCAKHHGIPFYVAAPLT 240

Query: 297 SIDLSLSSGEEIVIEERSPKELLNSRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITEK 356
           S+DLSLSSG+EIVIEERSPKEL+++ GGLGE++AA GI+VWNPAFD+TPA LIAGIITEK
Sbjct: 241 SVDLSLSSGKEIVIEERSPKELMHTHGGLGERIAAPGISVWNPAFDMTPAELIAGIITEK 300

Query: 357 GVITKAGSESFNIKEFVQKTAG 379
           GVITK G+++F+I  F +K  G
Sbjct: 301 GVITKNGNDTFDISSFAKKITG 322

BLAST of Spg022318 vs. TAIR 10
Match: AT3G07300.3 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 3.7e-11
Identity = 69/242 (28.51%), Postives = 101/242 (41.74%), Query Frame = 0

Query: 120 EIVSEAAAISSEAKSVVQAYIEAAEAMLEDDVASNKAIGSYGATYIRDQQKSLEKFSVLT 179
           E  S++A  SS  + +    IE    ++++    ++ I      +I              
Sbjct: 163 ESKSKSADKSSLTRKLKHDVIEGVNQLIQEIAGCHEQIAEQAIEHI-------------- 222

Query: 180 HCNTGSLATAGYGTALGVIRALHSAGVLERAYCTETRPFNQGSRLTAYELVHEKIPSTLI 239
           H N   L      T L  + A        R +  E  P  QG  L A ELV   + +T+I
Sbjct: 223 HQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVAEGAPRYQG-HLLAKELVARGLQTTVI 282

Query: 240 ADSAAAALMKFGRVNAVVVGADRVAANGDTANKIGTYSLALCAMHHKIPFFVAAPLTSID 299
            DSA  A++   RVN V++GA  V ANG     +G    AL A  H +PF V A    + 
Sbjct: 283 TDSAVFAMI--SRVNMVIIGAHAVMANGGVIGPVGVNMAALAAQKHAVPFVVLAGSHKLC 342

Query: 300 LSLSSGEEIVIEE-RSPKELLN---SRGGLGEQVAASGIAVWNPAFDVTPAYLIAGIITE 358
                  E+++ E RSP ELL+       L     +  + V NP FD  P  L++  IT+
Sbjct: 343 PLYPHNPEVLLNELRSPSELLDFGEFSDCLDFGAGSGSLQVVNPTFDYVPPNLVSLFITD 387

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008450940.13.4e-19295.24PREDICTED: methylthioribose-1-phosphate isomerase [Cucumis melo] >XP_016900996.1... [more]
XP_011660028.15.8e-19294.75methylthioribose-1-phosphate isomerase [Cucumis sativus] >KGN66312.1 hypothetica... [more]
XP_038880122.13.8e-19195.03methylthioribose-1-phosphate isomerase [Benincasa hispida] >XP_038880123.1 methy... [more]
XP_022147606.18.7e-18891.34methylthioribose-1-phosphate isomerase [Momordica charantia][more]
KAG6588092.11.2e-18491.88Methylthioribose-1-phosphate isomerase, partial [Cucurbita argyrosperma subsp. s... [more]
Match NameE-valueIdentityDescription
B9RR885.0e-16277.78Methylthioribose-1-phosphate isomerase OS=Ricinus communis OX=3988 GN=RCOM_07114... [more]
B9HCR23.2e-16180.27Methylthioribose-1-phosphate isomerase OS=Populus trichocarpa OX=3694 GN=POPTRDR... [more]
Q948X81.4e-15676.41Methylthioribose-1-phosphate isomerase OS=Nicotiana tabacum OX=4097 GN=CIG2 PE=2... [more]
Q9ZUG43.2e-15374.52Methylthioribose-1-phosphate isomerase OS=Arabidopsis thaliana OX=3702 GN=At2g05... [more]
D7TCD05.2e-15178.27Methylthioribose-1-phosphate isomerase OS=Vitis vinifera OX=29760 GN=VIT_11s0016... [more]
Match NameE-valueIdentityDescription
A0A1S3BPT01.7e-19295.24Methylthioribose-1-phosphate isomerase OS=Cucumis melo OX=3656 GN=LOC103492378 P... [more]
A0A5D3CHZ01.7e-19295.24Methylthioribose-1-phosphate isomerase OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A0A0LZL42.8e-19294.75Methylthioribose-1-phosphate isomerase OS=Cucumis sativus OX=3659 GN=Csa_1G59710... [more]
A0A6J1D2V94.2e-18891.34Methylthioribose-1-phosphate isomerase OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1F5M27.4e-18591.36Methylthioribose-1-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
Match NameE-valueIdentityDescription
AT2G05830.12.3e-15474.52NagB/RpiA/CoA transferase-like superfamily protein [more]
AT2G05830.42.7e-13474.92NagB/RpiA/CoA transferase-like superfamily protein [more]
AT2G05830.24.5e-13475.16NagB/RpiA/CoA transferase-like superfamily protein [more]
AT2G05830.34.5e-13475.16NagB/RpiA/CoA transferase-like superfamily protein [more]
AT3G07300.33.7e-1128.51NagB/RpiA/CoA transferase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042529Initiation factor 2B-like, C-terminalGENE3D3.40.50.10470coord: 208..373
e-value: 9.0E-58
score: 197.1
IPR027363Methylthioribose-1-phosphate isomerase, N-terminalGENE3D1.20.120.420coord: 15..207
e-value: 1.8E-69
score: 235.4
IPR011559Initiation factor 2B alpha/beta/deltaTIGRFAMTIGR00524TIGR00524coord: 44..361
e-value: 1.3E-107
score: 357.4
IPR000649Initiation factor 2B-relatedPFAMPF01008IF-2Bcoord: 59..360
e-value: 3.0E-78
score: 262.9
IPR005251Methylthioribose-1-phosphate isomeraseTIGRFAMTIGR00512TIGR00512coord: 18..361
e-value: 3.4E-142
score: 471.3
IPR005251Methylthioribose-1-phosphate isomeraseHAMAPMF_01678Salvage_MtnAcoord: 17..370
score: 71.951904
NoneNo IPR availablePANTHERPTHR43475:SF1METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASEcoord: 15..374
NoneNo IPR availablePANTHERPTHR43475METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASEcoord: 15..374
IPR037171NagB/RpiA transferase-likeSUPERFAMILY100950NagB/RpiA/CoA transferase-likecoord: 17..362

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg022318.1Spg022318.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019509 L-methionine salvage from methylthioadenosine
biological_process GO:0019284 L-methionine salvage from S-adenosylmethionine
biological_process GO:0044249 cellular biosynthetic process
biological_process GO:0044237 cellular metabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0046523 S-methyl-5-thioribose-1-phosphate isomerase activity