Spg021805 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg021805
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionisoleucine N-monooxygenase 2-like
Locationscaffold2: 13375271 .. 13379900 (-)
RNA-Seq ExpressionSpg021805
SyntenySpg021805
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGCCAGTGCCCTAGCTCTGTCTTCTTCAACCTTTCTTCTAGCTATTTGCAAGAGCTTCATGATCTTCTTGACTGGCTCATTCATGGCAGCTGTTCTCATCTGGTTGTTTTCCTATTATGGGAAAAGAAAGCAGTCTGTAGGCACTGCAGCTCTCCCACCCGGTCCGTCTCCATGGCCGATCGTCGGCTGCCTCCCGACGATATGGCGGAGGAAGCCGAGATACCGATGGATTCACAGCGTCATGGAAGACCTAAACACCGAAATCGCTTGCATCCGTCTCGGCAAAACCCATGTCATTCCTGTGATGTCTCCAGAACTCGCCGTCGAGTTTCTAAAGACGCACGACTCTCTATTTGCGTCGCGACCGATCACCGTCACCACCGACATGTTCAGCGACGGGTTCCTCACGGCAGGCGTCGCGCCGTGGGGAGACCAATGGAGGAAGATGAGAAGAGTCCTCACGTCGGAAATCCTCAGTCCGGCTCGACACCGGTGGATGCTCGCAAAGAGGACCGAAGAAGCCGACAATCTCCTACGATACGTGTTCCATTTAACGACGACGGGGAACGACGACGACGATGTTGGCGGAGGGGCAGTGGTTAACCTGAGAAGCATAACGCAGCATTATTCGGGGAACGTGATGCGACGAATGATGTTCGATAGGCGATACTACGGTGGAGGAAGAGCGGACGGGGGGCCGACGGCGGAGGAAGAAGAGCATATTGGAGCATTGTTTACGATGCTTCAACATGTTTATGCATTTTGTGTATCGGATTACATGCCGTGTTTGAAGGCGTTTGATTTGGATGGTCACGAGAGGACTGTTAAGAAGGCTTTGAATGTTATTAGAAAGTATGAAGAGCCCATCATTGACGAGAGAGTTCAGCAATGGAGAGATGGGAGGAGGACGGAGGCTGAGGACATGTTGGATATCTTCATCTCGCTCAAAGATGGCGATGGGAGGCCATTGTTGTCAGTCAGGGAGATTAAAGCACAGATTACAGTAAGAATTTGATTACAACTTTTTTTTTTTTTTTAATTATTTAGCTAGGTTTGCATGTGTCGTGTGGTGAATAATTAGGTTTGGTTTTAGTTTTTTTTCGTTTGTGAAGTTTTAGAGTGTTGTCTGATAACTGATAAGTGACTGATATACATGTTGTTTTCTATTTTTTTTAAAAAATGTTTTTGAAATTCTAGGTAGGGATGTCAAAAAGACTATGCAACCCAAACTGTAAGTGTTGGGTTGGGTTATGATTTTTTTCGGTTTGGGTTGAGTTGATTTTTTACAAAACTGAAATTTTGGTTCGGTTCACGAGTCTAAAAGATTTGGACCCATGCATTATTATTTTTTAAGAAATTTAAAACTCTATAATTAAGAAATTTTAAATAATTATTATTTTTAAAAAATAAAAAATTTTAAAAAAAATTAAGAAATTTTAAATAATTATTATTAAAAAAAAAAACCGGCAACCCAACCCAACCCAACTAAAAAATAGAAGAGTTAGGTTGGATTGGGTTGTCATCTACATACATTTTCGGTTCCTTCGGATAGCCAACTCGGCCAACCTAACCTAGATCTCTGTTTTTCAATAACCAAAAAGAAAAAAAAAGAGTTTTAAAAATGGATCTCTGTTTTTCAAAATCCCACGTTTTCTTCTAATGTTCAGTTTCTTGTTTGTTCTCTTTTGAATTCAAGAAGGAAAAAAAAAATCTATTTCACTTAGGGAAATTGCAATAGGTAGCAAATAACATTAGGATATTTACAAATTTAGCTATGTTTTTTTAGAATTCCAAATATAGAAAAATTTTTAAAATCTCATTTTCAATTTTTTTATTATGTTCATGAAATATACATCTAATCTACTATATATTTAGTATATCAATTGATTGATATACCAAATATCTAGTATATCTGTCACACTAAATAATTGAATGATGTAACTGATATACGGTAAATCGATAACACTATTGATATACTTTTTAAACTTCTCACTTCCAGCGTGTATTTTAGGGTTTTTTTTTTTTTTTACTTTTCAACTTTAATGTTTAGGTTGGACCAACTTTGTTTTGTCTTTTTTTTTTTTGTTATATTTGCAACATTAAAAAACTGATGACATGGTTGCAAATTTCATTTTTATTTTTTCTATTCTTGCAACTATCCTTTCACTTATTGTTTTCCAAAAAAAATCACTAACTTTTCTCTCTCTAAAATCTTTCCATAAATTTCTCTTTCCTAAATTTATCACTCTAAGGTTTCTTTCTAACTTTCTCTCTGATCTCTATCTATCTGATTTTTATTTTATTTTTCTCCCTCTAGCCCTCTGTGTCTCTAATTTTTTGAAAACAAAAATGTCAAAAACTATTATGACACAAAAAAAAAAAATAAAATAAAATAAAACAAGAAATGAAAAAACCAAATAAAATAGTTATTGAATGAGACTTTAATAAAATCTAAATTTATTTTGAAATAAATAAGAAATTAAAACAAAATGTTTAACGATCAAAATAAAATGAACACAAAAATTGAAGAATACTTGGCTACATAATGGTGTGTAGGTATCTTTTTACACATCCCATTAAAATCTTGACAATTAAAGGAGAGAAAGTAGAAAATGAGAAAATTTTGTTATCTCTTATAGTTGTCAATATTTTAATGGTGGTGTGTAGGAAGATATCTACTAGCTAAGTATTTTCCACAAAAATTTAAATCTTATTACTATTATTTATGTTAAGTTCAAACTTATCATACTTTGACTTTTTGTTGGTTGGATAAAGAAATGTTTTTGTTTTTGCTACTGAAATTTGATTGAGATTTTTTTTTAGTTCATTTGATTAAGATTTTAAAAGCATTGTTTTTAAATGTGAGAAACAAAATAAAGAAATTGTTTTTAACTAAGATGATGTTTTTGTTTTTTGTTTTAAAAAATCATGCTTTCGTTAACGTTTTTTCTAATCTATTTTTATAAAATAAAAGTTTGAACCCTAACCAAATACTGATAATTTCTTTTTTTTATCACAAAGCAGCCTAAGACGCTTGGGACATTTTTTATTAAATACACCAACATATTTTCAGTTTTTCTTTTGTTTTTAAACCCTAACCAAATACTGATAATTTCTTTTCAGTTTTTGTTTTGTTTTTAAATACACCACAACATATTTTTTTTCGAACTTTTCTTGTTTTCAGTTTTTGTTTTTGTTTTTTAGATAAATTTTTGTTTCTTTTTTAAAATACACCAATTTTCTTTTTAAAATTAAAATTTATATAATTTGAGATTTAAATACTTTCACTCCTCTTATCAAACAAAATAATTATTGTGGGTGCTTTTATCATTAATAAATAATAATTATTTAAAATTTTATAATAATAAATAAATAAGAAACATCAACCTCTTTTTCCATTTTTTATTTGATAAGAGAAGTGAAAATATTTAAATTTCAAATTATATAAATTTTAATTTTAAAAAGAAAATTGGAAAAAAAAATTGTTTGTGTATTTTAAAAAAGAAACAAAAACTTATCTAAAAAAAGAGAAAAAAAGAAAAAAAGAAACAAAAACTGAAGAGTAAAAAACATAAAAATGAAAAGGAAAGTTGCACACATGCCAAGATCGATTTCGTCTACGGATGGATTATAGCTTGAGAAGACACCACTTTGACCAAATACATAATTGTTTTCAAATTGCATATTGGTCGTCTCTACCATCTCCAGCGACATGTTGACACATGGCATAGGAAACATAAGAGCGCGAATTTAAAATTACAATATTTAAATAATAAGATCGAAAACTATTATATTTTAATAAAAAGTTTCAAAAAATTATAAAATTAATAAAAAATGTCCTAAGACGCTTGTATCTTTTTCTACTTATGATTTTGATTAAGAAGTATTTAGGGTCATAATATAAAAGCACTTTCAAACATAGCGTATGATTGATTTGAAGTAAGAAAGTGCTTTTGTTGTTGAAATGACAGGAACTGCTTCTTGCAACAGTGGACAATCCATCGAACGCAGTGGAATGGGCCATAGCAGAGATGCTAAACCAGCCCCAAATCCTCCAAACCGCCACCGAAGAACTTGACAGAGTCGTCGGAAAACAGCGCCTCGTCCAAGAATCCGACCTCCCAAATCTCAAATTCCTCACCGCCTGCGCCAGAGAGGCCCTCCGCCTCCACCCAATCGCCCCCTTCAACCTCCCTCACATCGCCACCGCCGATGCCGTCGTCGCCGGCTACTTCATCCCCGCCGGCAGCCACGTCCTCCTAAGCCGCCTTGGACTCGGTCGGAACCCTAGGATTTGGCCCGACCCGATGCGATTCGATCCGAACCGCCATCTCCAAGATCCGGGCGCCGATCCCAACTTGGGGGAGCCTGATCTGAGGTTTATTACGTTCACCAGAGGGCGGAGAGGGTGTATGGGCAGCGCACTTGGGACTGCCATTACGATGATGCTGTTGGCTAGGCTTGTGCAGGGATTTTCTTGGCGGTTGCCGCCCGGTGCGGCGGAGATTGACCTGTCGGAGTCTGACCAGTTGTTTCTGAAGAATCCTCTGTTCGTGTTTGCAGAGCCGCGGCTGCTGGAATCTCTGTACCCTGATTCCGAAACAGGGGAGAAACTTTTTTTATAG

mRNA sequence

ATGGCAGCCAGTGCCCTAGCTCTGTCTTCTTCAACCTTTCTTCTAGCTATTTGCAAGAGCTTCATGATCTTCTTGACTGGCTCATTCATGGCAGCTGTTCTCATCTGGTTGTTTTCCTATTATGGGAAAAGAAAGCAGTCTGTAGGCACTGCAGCTCTCCCACCCGGTCCGTCTCCATGGCCGATCGTCGGCTGCCTCCCGACGATATGGCGGAGGAAGCCGAGATACCGATGGATTCACAGCGTCATGGAAGACCTAAACACCGAAATCGCTTGCATCCGTCTCGGCAAAACCCATGTCATTCCTGTGATGTCTCCAGAACTCGCCGTCGAGTTTCTAAAGACGCACGACTCTCTATTTGCGTCGCGACCGATCACCGTCACCACCGACATGTTCAGCGACGGGTTCCTCACGGCAGGCGTCGCGCCGTGGGGAGACCAATGGAGGAAGATGAGAAGAGTCCTCACGTCGGAAATCCTCAGTCCGGCTCGACACCGGTGGATGCTCGCAAAGAGGACCGAAGAAGCCGACAATCTCCTACGATACGTGTTCCATTTAACGACGACGGGGAACGACGACGACGATGTTGGCGGAGGGGCAGTGGTTAACCTGAGAAGCATAACGCAGCATTATTCGGGGAACGTGATGCGACGAATGATGTTCGATAGGCGATACTACGGTGGAGGAAGAGCGGACGGGGGGCCGACGGCGGAGGAAGAAGAGCATATTGGAGCATTGTTTACGATGCTTCAACATGTTTATGCATTTTGTGTATCGGATTACATGCCGTGTTTGAAGGCGTTTGATTTGGATGGTCACGAGAGGACTGTTAAGAAGGCTTTGAATGTTATTAGAAAGTATGAAGAGCCCATCATTGACGAGAGAGTTCAGCAATGGAGAGATGGGAGGAGGACGGAGGCTGAGGACATGTTGGATATCTTCATCTCGCTCAAAGATGGCGATGGGAGGCCATTGTTGTCAGTCAGGGAGATTAAAGCACAGATTACAGAACTGCTTCTTGCAACAGTGGACAATCCATCGAACGCAGTGGAATGGGCCATAGCAGAGATGCTAAACCAGCCCCAAATCCTCCAAACCGCCACCGAAGAACTTGACAGAGTCGTCGGAAAACAGCGCCTCGTCCAAGAATCCGACCTCCCAAATCTCAAATTCCTCACCGCCTGCGCCAGAGAGGCCCTCCGCCTCCACCCAATCGCCCCCTTCAACCTCCCTCACATCGCCACCGCCGATGCCGTCGTCGCCGGCTACTTCATCCCCGCCGGCAGCCACGTCCTCCTAAGCCGCCTTGGACTCGGTCGGAACCCTAGGATTTGGCCCGACCCGATGCGATTCGATCCGAACCGCCATCTCCAAGATCCGGGCGCCGATCCCAACTTGGGGGAGCCTGATCTGAGGTTTATTACGTTCACCAGAGGGCGGAGAGGGTGTATGGGCAGCGCACTTGGGACTGCCATTACGATGATGCTGTTGGCTAGGCTTGTGCAGGGATTTTCTTGGCGGTTGCCGCCCGGTGCGGCGGAGATTGACCTGTCGGAGTCTGACCAGTTGTTTCTGAAGAATCCTCTGTTCGTGTTTGCAGAGCCGCGGCTGCTGGAATCTCTGTACCCTGATTCCGAAACAGGGGAGAAACTTTTTTTATAG

Coding sequence (CDS)

ATGGCAGCCAGTGCCCTAGCTCTGTCTTCTTCAACCTTTCTTCTAGCTATTTGCAAGAGCTTCATGATCTTCTTGACTGGCTCATTCATGGCAGCTGTTCTCATCTGGTTGTTTTCCTATTATGGGAAAAGAAAGCAGTCTGTAGGCACTGCAGCTCTCCCACCCGGTCCGTCTCCATGGCCGATCGTCGGCTGCCTCCCGACGATATGGCGGAGGAAGCCGAGATACCGATGGATTCACAGCGTCATGGAAGACCTAAACACCGAAATCGCTTGCATCCGTCTCGGCAAAACCCATGTCATTCCTGTGATGTCTCCAGAACTCGCCGTCGAGTTTCTAAAGACGCACGACTCTCTATTTGCGTCGCGACCGATCACCGTCACCACCGACATGTTCAGCGACGGGTTCCTCACGGCAGGCGTCGCGCCGTGGGGAGACCAATGGAGGAAGATGAGAAGAGTCCTCACGTCGGAAATCCTCAGTCCGGCTCGACACCGGTGGATGCTCGCAAAGAGGACCGAAGAAGCCGACAATCTCCTACGATACGTGTTCCATTTAACGACGACGGGGAACGACGACGACGATGTTGGCGGAGGGGCAGTGGTTAACCTGAGAAGCATAACGCAGCATTATTCGGGGAACGTGATGCGACGAATGATGTTCGATAGGCGATACTACGGTGGAGGAAGAGCGGACGGGGGGCCGACGGCGGAGGAAGAAGAGCATATTGGAGCATTGTTTACGATGCTTCAACATGTTTATGCATTTTGTGTATCGGATTACATGCCGTGTTTGAAGGCGTTTGATTTGGATGGTCACGAGAGGACTGTTAAGAAGGCTTTGAATGTTATTAGAAAGTATGAAGAGCCCATCATTGACGAGAGAGTTCAGCAATGGAGAGATGGGAGGAGGACGGAGGCTGAGGACATGTTGGATATCTTCATCTCGCTCAAAGATGGCGATGGGAGGCCATTGTTGTCAGTCAGGGAGATTAAAGCACAGATTACAGAACTGCTTCTTGCAACAGTGGACAATCCATCGAACGCAGTGGAATGGGCCATAGCAGAGATGCTAAACCAGCCCCAAATCCTCCAAACCGCCACCGAAGAACTTGACAGAGTCGTCGGAAAACAGCGCCTCGTCCAAGAATCCGACCTCCCAAATCTCAAATTCCTCACCGCCTGCGCCAGAGAGGCCCTCCGCCTCCACCCAATCGCCCCCTTCAACCTCCCTCACATCGCCACCGCCGATGCCGTCGTCGCCGGCTACTTCATCCCCGCCGGCAGCCACGTCCTCCTAAGCCGCCTTGGACTCGGTCGGAACCCTAGGATTTGGCCCGACCCGATGCGATTCGATCCGAACCGCCATCTCCAAGATCCGGGCGCCGATCCCAACTTGGGGGAGCCTGATCTGAGGTTTATTACGTTCACCAGAGGGCGGAGAGGGTGTATGGGCAGCGCACTTGGGACTGCCATTACGATGATGCTGTTGGCTAGGCTTGTGCAGGGATTTTCTTGGCGGTTGCCGCCCGGTGCGGCGGAGATTGACCTGTCGGAGTCTGACCAGTTGTTTCTGAAGAATCCTCTGTTCGTGTTTGCAGAGCCGCGGCTGCTGGAATCTCTGTACCCTGATTCCGAAACAGGGGAGAAACTTTTTTTATAG

Protein sequence

MAASALALSSSTFLLAICKSFMIFLTGSFMAAVLIWLFSYYGKRKQSVGTAALPPGPSPWPIVGCLPTIWRRKPRYRWIHSVMEDLNTEIACIRLGKTHVIPVMSPELAVEFLKTHDSLFASRPITVTTDMFSDGFLTAGVAPWGDQWRKMRRVLTSEILSPARHRWMLAKRTEEADNLLRYVFHLTTTGNDDDDVGGGAVVNLRSITQHYSGNVMRRMMFDRRYYGGGRADGGPTAEEEEHIGALFTMLQHVYAFCVSDYMPCLKAFDLDGHERTVKKALNVIRKYEEPIIDERVQQWRDGRRTEAEDMLDIFISLKDGDGRPLLSVREIKAQITELLLATVDNPSNAVEWAIAEMLNQPQILQTATEELDRVVGKQRLVQESDLPNLKFLTACAREALRLHPIAPFNLPHIATADAVVAGYFIPAGSHVLLSRLGLGRNPRIWPDPMRFDPNRHLQDPGADPNLGEPDLRFITFTRGRRGCMGSALGTAITMMLLARLVQGFSWRLPPGAAEIDLSESDQLFLKNPLFVFAEPRLLESLYPDSETGEKLFL
Homology
BLAST of Spg021805 vs. NCBI nr
Match: TYK27737.1 (tyrosine N-monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 893.6 bits (2308), Expect = 8.3e-256
Identity = 448/549 (81.60%), Postives = 477/549 (86.89%), Query Frame = 0

Query: 8   LSSSTFLLAICKSFMIFLTGSFMAAVLIWLFSYYGKR--KQSVGT--AALPPGPSPWPIV 67
           LSSST        FMI   GS M    I    +YGKR  K  VG+  AALPPGPSPWPIV
Sbjct: 7   LSSST--------FMILFYGSIMVVAFI---LFYGKRRNKYCVGSTAAALPPGPSPWPIV 66

Query: 68  GCLPTIWRRKPRYRWIHSVMEDLNTEIACIRLGKTHVIPVMSPELAVEFLKTHDSLFASR 127
           GCLPT+WR+KPRYRW+H +ME+LNTEIACIR+GK HVIPV SPELA+EFLKTHDS+FASR
Sbjct: 67  GCLPTLWRKKPRYRWVHKIMEELNTEIACIRIGKIHVIPVTSPELAMEFLKTHDSVFASR 126

Query: 128 PITVTTDMFSDGFLTAGVAPWGDQWRKMRRVLTSEILSPARHRWMLAKRTEEADNLLRYV 187
           PITVTTDMFSDGFLTAGVAPWG+QW+KMRR+LTSEILSPARHRWML+KRTEEADNLLRYV
Sbjct: 127 PITVTTDMFSDGFLTAGVAPWGNQWKKMRRILTSEILSPARHRWMLSKRTEEADNLLRYV 186

Query: 188 FHLTTTGNDDDDVGGGAVVNLRSITQHYSGNVMRRMMFDRRYYGGGRADGGPTAEEEEHI 247
           F LT T            V++RSITQHYSGNVMRRMMF+RRY+G GRADGGP  EEEEHI
Sbjct: 187 FSLTKTTPTS--------VSVRSITQHYSGNVMRRMMFNRRYFGKGRADGGPATEEEEHI 246

Query: 248 GALFTMLQHVYAFCVSDYMPCLKAFDLDGHERTVKKALNVIRKYEEPIIDERVQQWRDGR 307
           GALFTMLQHVYAFCVSDYM CLKAFDLDGHER VKKALNVIRKYEEPIIDERVQQWRDG+
Sbjct: 247 GALFTMLQHVYAFCVSDYMSCLKAFDLDGHERIVKKALNVIRKYEEPIIDERVQQWRDGK 306

Query: 308 RTEAEDMLDIFISLKDGDGRPLLSVREIKAQITELLLATVDNPSNAVEWAIAEMLNQPQI 367
           R EAEDMLDIFISLKD  G PLLSV+EIKAQITELLLATVDNPSNAVEWA+AEMLNQPQ 
Sbjct: 307 RIEAEDMLDIFISLKDESGEPLLSVKEIKAQITELLLATVDNPSNAVEWAMAEMLNQPQH 366

Query: 368 LQTATEELDRVVGKQRLVQESDLPNLKFLTACAREALRLHPIAPFNLPHIATADAVVAGY 427
           LQ ATEELDRVVGK+RLVQESD+PNLKFLTACAREALRLHPIAPFNLPHIAT D VVAGY
Sbjct: 367 LQQATEELDRVVGKERLVQESDIPNLKFLTACAREALRLHPIAPFNLPHIATTDVVVAGY 426

Query: 428 FIPAGSHVLLSRLGLGRNPRIWPDPMRFDPNRHLQDPGADPNLGEPDLRFITFTRGRRGC 487
            IPAGSHVLLSRLGLGRN RIWPDPMRFDPNRHLQDPGAD  L EPDLRFITFTRGRRGC
Sbjct: 427 LIPAGSHVLLSRLGLGRNHRIWPDPMRFDPNRHLQDPGADLGLAEPDLRFITFTRGRRGC 486

Query: 488 MGSALGTAITMMLLARLVQGFSWRLPPGAAEIDLSESDQLFLKNPLFVFAEPRLLESLYP 547
           MG  LGTAITMMLLARLVQGF+W+LPPG A I+LSESDQLFLK+PLF  A+PRL ESLYP
Sbjct: 487 MGGTLGTAITMMLLARLVQGFTWQLPPGVAAIELSESDQLFLKDPLFALAQPRLPESLYP 536

Query: 548 DSETGEKLF 553
           D E  +K F
Sbjct: 547 DFEIEKKDF 536

BLAST of Spg021805 vs. NCBI nr
Match: KAA0056052.1 (tyrosine N-monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 889.4 bits (2297), Expect = 1.6e-254
Identity = 446/549 (81.24%), Postives = 476/549 (86.70%), Query Frame = 0

Query: 8   LSSSTFLLAICKSFMIFLTGSFMAAVLIWLFSYYGKR--KQSVGT--AALPPGPSPWPIV 67
           LSSST        FMI   GS M    I    +YGKR  K  VG+  AALPPGP PWPIV
Sbjct: 7   LSSST--------FMILFYGSIMVVAFI---LFYGKRRNKYCVGSTAAALPPGPIPWPIV 66

Query: 68  GCLPTIWRRKPRYRWIHSVMEDLNTEIACIRLGKTHVIPVMSPELAVEFLKTHDSLFASR 127
           GCLPT+WR+KPRYRW+H +ME+LNTEIACIR+GK HVIPV SPELA+EFLKTHDS+FASR
Sbjct: 67  GCLPTLWRKKPRYRWVHKIMEELNTEIACIRIGKIHVIPVTSPELAMEFLKTHDSVFASR 126

Query: 128 PITVTTDMFSDGFLTAGVAPWGDQWRKMRRVLTSEILSPARHRWMLAKRTEEADNLLRYV 187
           PITVTTDMFSDGFLTAGVAPWG+QW+KMRR+LTSEILSPARHRWML+KRTEEADNLLRYV
Sbjct: 127 PITVTTDMFSDGFLTAGVAPWGNQWKKMRRILTSEILSPARHRWMLSKRTEEADNLLRYV 186

Query: 188 FHLTTTGNDDDDVGGGAVVNLRSITQHYSGNVMRRMMFDRRYYGGGRADGGPTAEEEEHI 247
           F LT T            V++RSITQHYSGNVMRRMMF+RRY+G GRADGGP  EEEEHI
Sbjct: 187 FSLTKTTPTS--------VSVRSITQHYSGNVMRRMMFNRRYFGKGRADGGPATEEEEHI 246

Query: 248 GALFTMLQHVYAFCVSDYMPCLKAFDLDGHERTVKKALNVIRKYEEPIIDERVQQWRDGR 307
           GALFTMLQHVYAFCVSDYM CLKAFDLDGHER VKKALNVIRKYEEPIIDERVQQWRDG+
Sbjct: 247 GALFTMLQHVYAFCVSDYMSCLKAFDLDGHERIVKKALNVIRKYEEPIIDERVQQWRDGK 306

Query: 308 RTEAEDMLDIFISLKDGDGRPLLSVREIKAQITELLLATVDNPSNAVEWAIAEMLNQPQI 367
           R EAEDMLDIFISLKD  G PLLSV+EIKAQITELLLATVDNPSNAVEWA+AEMLNQPQ 
Sbjct: 307 RIEAEDMLDIFISLKDESGEPLLSVKEIKAQITELLLATVDNPSNAVEWAMAEMLNQPQH 366

Query: 368 LQTATEELDRVVGKQRLVQESDLPNLKFLTACAREALRLHPIAPFNLPHIATADAVVAGY 427
           LQ ATEELDRVVGK+RLVQESD+PNLKFLTACAREALRLHPIAPFNLPHIAT D VVAGY
Sbjct: 367 LQQATEELDRVVGKERLVQESDIPNLKFLTACAREALRLHPIAPFNLPHIATTDVVVAGY 426

Query: 428 FIPAGSHVLLSRLGLGRNPRIWPDPMRFDPNRHLQDPGADPNLGEPDLRFITFTRGRRGC 487
            IPAGSHVLLSRLGLGRN RIWPDPMRFDPNRHLQDPGAD  L EPDLRFITFTRGRRGC
Sbjct: 427 LIPAGSHVLLSRLGLGRNHRIWPDPMRFDPNRHLQDPGADLGLAEPDLRFITFTRGRRGC 486

Query: 488 MGSALGTAITMMLLARLVQGFSWRLPPGAAEIDLSESDQLFLKNPLFVFAEPRLLESLYP 547
           MG  LGTAITMMLLARLVQGF+W+LPPG A I+LSESDQLFLK+PLF  A+PRL ESLYP
Sbjct: 487 MGGTLGTAITMMLLARLVQGFTWQLPPGVAAIELSESDQLFLKDPLFALAQPRLPESLYP 536

Query: 548 DSETGEKLF 553
           + E  +K F
Sbjct: 547 NFEIEKKDF 536

BLAST of Spg021805 vs. NCBI nr
Match: XP_004139121.1 (tyrosine N-monooxygenase [Cucumis sativus] >KGN66827.2 hypothetical protein Csa_007093 [Cucumis sativus])

HSP 1 Score: 888.3 bits (2294), Expect = 3.5e-254
Identity = 444/549 (80.87%), Postives = 477/549 (86.89%), Query Frame = 0

Query: 6   LALSSSTFLLAICKSFMIFLTGSFMAAVLIWLFSYYGKRKQSVGT--AALPPGPSPWPIV 65
           L LSSSTF++       I   GS +    I LF    + K  VG+  AALPPGP+PWPIV
Sbjct: 7   LPLSSSTFMI-------ILFYGSILVVAFI-LFYAKRRNKYCVGSTAAALPPGPTPWPIV 66

Query: 66  GCLPTIWRRKPRYRWIHSVMEDLNTEIACIRLGKTHVIPVMSPELAVEFLKTHDSLFASR 125
           GCLPTIWR+KPRYRW+H +ME+LNTEIACIRLGK HVIPV SPELA+EFLKTHDS+FASR
Sbjct: 67  GCLPTIWRKKPRYRWVHKIMEELNTEIACIRLGKIHVIPVTSPELAIEFLKTHDSVFASR 126

Query: 126 PITVTTDMFSDGFLTAGVAPWGDQWRKMRRVLTSEILSPARHRWMLAKRTEEADNLLRYV 185
           PITVTTD+FSDGFLTAGVAPWG+QW+KMRR+LTSEILSPARHR ML+KRTEEADNLLRYV
Sbjct: 127 PITVTTDIFSDGFLTAGVAPWGNQWKKMRRILTSEILSPARHRSMLSKRTEEADNLLRYV 186

Query: 186 FHLTTTGNDDDDVGGGAVVNLRSITQHYSGNVMRRMMFDRRYYGGGRADGGPTAEEEEHI 245
           F LT T            VN+RSITQHYSGNVMRRMMF+RRYYG GR DGGP  EEEEHI
Sbjct: 187 FSLTKTTPTS--------VNVRSITQHYSGNVMRRMMFNRRYYGKGRPDGGPATEEEEHI 246

Query: 246 GALFTMLQHVYAFCVSDYMPCLKAFDLDGHERTVKKALNVIRKYEEPIIDERVQQWRDGR 305
           GALFTMLQHVYAFCVSDYM CLKAFDLDGHER VKKALNVIRKYEEPIIDERVQQWRDG+
Sbjct: 247 GALFTMLQHVYAFCVSDYMSCLKAFDLDGHERIVKKALNVIRKYEEPIIDERVQQWRDGK 306

Query: 306 RTEAEDMLDIFISLKDGDGRPLLSVREIKAQITELLLATVDNPSNAVEWAIAEMLNQPQI 365
           R E EDMLDIFISLKDG+G PLLSV+EIKAQITELLLATVDNPSNAVEWA+AEMLNQPQ+
Sbjct: 307 RKETEDMLDIFISLKDGNGEPLLSVKEIKAQITELLLATVDNPSNAVEWAMAEMLNQPQL 366

Query: 366 LQTATEELDRVVGKQRLVQESDLPNLKFLTACAREALRLHPIAPFNLPHIATADAVVAGY 425
           LQ ATEELDRVVGKQRLVQESD+PNLKFLTACAREALRLHPIAPFNLPHIAT D VVAGY
Sbjct: 367 LQQATEELDRVVGKQRLVQESDIPNLKFLTACAREALRLHPIAPFNLPHIATTDVVVAGY 426

Query: 426 FIPAGSHVLLSRLGLGRNPRIWPDPMRFDPNRHLQDPGADPNLGEPDLRFITFTRGRRGC 485
            IPAGSHVLLSRLGLGRN RIWPDPMRFDPNRHLQDPGAD  LGEPDLRFITFTRGRRGC
Sbjct: 427 LIPAGSHVLLSRLGLGRNHRIWPDPMRFDPNRHLQDPGADLGLGEPDLRFITFTRGRRGC 486

Query: 486 MGSALGTAITMMLLARLVQGFSWRLPPGAAEIDLSESDQLFLKNPLFVFAEPRLLESLYP 545
           MG  LGTAITMMLLARLVQGF+W+LPPG A I+LSES QLFLK+P+F  A+PRL ESLYP
Sbjct: 487 MGGTLGTAITMMLLARLVQGFTWQLPPGVAAIELSESHQLFLKDPMFALAQPRLPESLYP 539

Query: 546 DSETGEKLF 553
           + E  +K F
Sbjct: 547 NFEIEKKEF 539

BLAST of Spg021805 vs. NCBI nr
Match: XP_008450479.1 (PREDICTED: tyrosine N-monooxygenase [Cucumis melo])

HSP 1 Score: 886.3 bits (2289), Expect = 1.3e-253
Identity = 434/517 (83.95%), Postives = 464/517 (89.75%), Query Frame = 0

Query: 40  YYGKR--KQSVGT--AALPPGPSPWPIVGCLPTIWRRKPRYRWIHSVMEDLNTEIACIRL 99
           +YGKR  K  VG+  AALPPGP PWPIVGCLPT+WR+KPRYRW+H +ME+LNTEIACIR+
Sbjct: 8   FYGKRRNKYCVGSTAAALPPGPIPWPIVGCLPTLWRKKPRYRWVHKIMEELNTEIACIRI 67

Query: 100 GKTHVIPVMSPELAVEFLKTHDSLFASRPITVTTDMFSDGFLTAGVAPWGDQWRKMRRVL 159
           GK HVIPV SPELA+EFLKTHDS+FASRPITVTTDMFSDGFLTAGVAPWG+QW+KMRR+L
Sbjct: 68  GKIHVIPVTSPELAMEFLKTHDSVFASRPITVTTDMFSDGFLTAGVAPWGNQWKKMRRIL 127

Query: 160 TSEILSPARHRWMLAKRTEEADNLLRYVFHLTTTGNDDDDVGGGAVVNLRSITQHYSGNV 219
           TSEILSPARHRWML+KRTEEADNLLRYVF LT T            V++RSITQHYSGNV
Sbjct: 128 TSEILSPARHRWMLSKRTEEADNLLRYVFSLTKTTPTS--------VSVRSITQHYSGNV 187

Query: 220 MRRMMFDRRYYGGGRADGGPTAEEEEHIGALFTMLQHVYAFCVSDYMPCLKAFDLDGHER 279
           MRRMMF+RRY+G GRADGGP  EEEEHIGALFTMLQHVYAFCVSDYM CLKAFDLDGHER
Sbjct: 188 MRRMMFNRRYFGKGRADGGPATEEEEHIGALFTMLQHVYAFCVSDYMSCLKAFDLDGHER 247

Query: 280 TVKKALNVIRKYEEPIIDERVQQWRDGRRTEAEDMLDIFISLKDGDGRPLLSVREIKAQI 339
            VKKALNVIRKYEEPIIDERVQQWRDG+R EAEDMLDIFISLKD  G PLLSV+EIKAQI
Sbjct: 248 IVKKALNVIRKYEEPIIDERVQQWRDGKRIEAEDMLDIFISLKDESGEPLLSVKEIKAQI 307

Query: 340 TELLLATVDNPSNAVEWAIAEMLNQPQILQTATEELDRVVGKQRLVQESDLPNLKFLTAC 399
           TELLLATVDNPSNAVEWA+AEMLNQPQ LQ ATEELDRVVGK+RLVQESD+PNLKFLTAC
Sbjct: 308 TELLLATVDNPSNAVEWAMAEMLNQPQHLQQATEELDRVVGKERLVQESDIPNLKFLTAC 367

Query: 400 AREALRLHPIAPFNLPHIATADAVVAGYFIPAGSHVLLSRLGLGRNPRIWPDPMRFDPNR 459
           AREALRLHPIAPFNLPHIAT D VVAGY IPAGSHVLLSRLGLGRN RIWPDPMRFDPNR
Sbjct: 368 AREALRLHPIAPFNLPHIATTDVVVAGYLIPAGSHVLLSRLGLGRNHRIWPDPMRFDPNR 427

Query: 460 HLQDPGADPNLGEPDLRFITFTRGRRGCMGSALGTAITMMLLARLVQGFSWRLPPGAAEI 519
           HLQDPGAD  L EPDLRFITFTRGRRGCMG  LGTAITMMLLARLVQGF+W+LPPG A I
Sbjct: 428 HLQDPGADLGLAEPDLRFITFTRGRRGCMGGTLGTAITMMLLARLVQGFTWQLPPGVAAI 487

Query: 520 DLSESDQLFLKNPLFVFAEPRLLESLYPDSETGEKLF 553
           +LSESDQLFLK+PLF  A+PRL ESLYP+ E  +K F
Sbjct: 488 ELSESDQLFLKDPLFALAQPRLPESLYPNFEIEKKDF 516

BLAST of Spg021805 vs. NCBI nr
Match: KAG2675236.1 (hypothetical protein I3760_13G174900 [Carya illinoinensis])

HSP 1 Score: 733.0 bits (1891), Expect = 1.9e-207
Identity = 345/500 (69.00%), Postives = 418/500 (83.60%), Query Frame = 0

Query: 46  QSVGTAALPPGPSPWPIVGCLPTIWRRKPRYRWIHSVMEDLNTEIACIRLGKTHVIPVMS 105
           +S   A LPPGP PWPIVG LP +++ KPRY+W+H +M++ NTEIACIRLG+ HVIPV S
Sbjct: 2   KSKRVAPLPPGPKPWPIVGNLPELFKNKPRYKWLHRLMKEFNTEIACIRLGRVHVIPVAS 61

Query: 106 PELAVEFLKTHDSLFASRPITVTTDMFSDGFLTAGVAPWGDQWRKMRRVLTSEILSPARH 165
           PE+++EFLK +D++FASRPITVTT+MFS+GFLTAGV+PWGDQW+KMRRVLTSEILSPAR 
Sbjct: 62  PEISMEFLKKYDAVFASRPITVTTNMFSNGFLTAGVSPWGDQWKKMRRVLTSEILSPARL 121

Query: 166 RWMLAKRTEEADNLLRYVFHLTTTGNDDDDVG-GGAVVNLRSITQHYSGNVMRRMMFDRR 225
           +W+LAKR EEADNL+R ++++       D     G VV++R+++QH++GNVMRRM+F++R
Sbjct: 122 KWLLAKRNEEADNLVRVIYNMCKGSAISDTCSTSGTVVDVRTVSQHFTGNVMRRMIFNKR 181

Query: 226 YYGGGRADGGPTAEEEEHIGALFTMLQHVYAFCVSDYMPCLKAFDLDGHERTVKKALNVI 285
           YYG GR DGGP  EE+EHI ALF ML +VYAFCVSDYM  LK FDLDGHE+ V+ AL VI
Sbjct: 182 YYGKGREDGGPCFEEQEHIQALFAMLLYVYAFCVSDYMSILKPFDLDGHEKVVRNALKVI 241

Query: 286 RKYEEPIIDERVQQWRDGRRTEAEDMLDIFISLKDGDGRPLLSVREIKAQITELLLATVD 345
           +KYE+PI+DERVQ W++G+RTE ED+LDIFIS+KD DG PLLS+ EIKAQITELLLATVD
Sbjct: 242 KKYEDPIVDERVQMWKEGKRTEPEDLLDIFISIKDDDGNPLLSIEEIKAQITELLLATVD 301

Query: 346 NPSNAVEWAIAEMLNQPQILQTATEELDRVVGKQRLVQESDLPNLKFLTACAREALRLHP 405
           NPSNA EWA+AEMLNQP+ +Q A EE+DRVVGK+RLVQESDLP L +LTACAREALR+HP
Sbjct: 302 NPSNAAEWALAEMLNQPEQIQKAVEEIDRVVGKERLVQESDLPKLPYLTACAREALRIHP 361

Query: 406 IAPFNLPHIATADAVVAGYFIPAGSHVLLSRLGLGRNPRIWPDPMRFDPNRHLQDPGADP 465
           IAPFNLPH++TA+  VAGYFIP GS VLLSRLGLGRNPR+W +P+RFDP RHL+D   + 
Sbjct: 362 IAPFNLPHVSTAEVTVAGYFIPKGSQVLLSRLGLGRNPRVWEEPLRFDPERHLKDNLCEL 421

Query: 466 NLGEPDLRFITFTRGRRGCMGSALGTAITMMLLARLVQGFSWRLPP--GAAEIDLSESDQ 525
            L EP+LRFITFTRGRRGCMG  LGT IT+MLLARL+QGF W +PP     +IDL+E+DQ
Sbjct: 422 GLAEPELRFITFTRGRRGCMGGTLGTLITLMLLARLLQGFKWSIPPNNNMEKIDLTENDQ 481

Query: 526 LFLKNPLFVFAEPRLLESLY 543
           LFL  PL  +AEPRL   LY
Sbjct: 482 LFLAEPLQAYAEPRLPPHLY 501

BLAST of Spg021805 vs. ExPASy Swiss-Prot
Match: A0A068Q605 (Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 8.8e-184
Identity = 306/526 (58.17%), Postives = 402/526 (76.43%), Query Frame = 0

Query: 23  IFLTGSFMAAVLIWLFSYYGKRKQSVGTAALPPGPSPWPIVGCLPTIWRRKPRYRWIHSV 82
           I L+ +F+  + +  F      K SV    LPPGPSPWPIVG LP +WR +P +RWIHS+
Sbjct: 16  ISLSLAFIIFMFLVKFILKTHNKNSVPVVPLPPGPSPWPIVGSLPEMWRNRPAHRWIHSL 75

Query: 83  MEDLNTEIACIRLGKTHVIPVMSPELAVEFLKTHDSLFASRPITVTTDMFSDGFLTAGVA 142
           M+ LNT+IACIRLG  HVIPV SPE+A EFLK +D++FASRP+T+ T   S G+LT  V 
Sbjct: 76  MKKLNTDIACIRLGNVHVIPVTSPEIAREFLKKNDAVFASRPVTMATKTLSSGYLTTVVG 135

Query: 143 PWGDQWRKMRRVLTSEILSPARHRWMLAKRTEEADNLLRYVFHLTTTGNDDDDVGGGAVV 202
           PWGDQWRKMRRVL +E  +P+R  W+L KR EEADNL++++++  +   +      GAVV
Sbjct: 136 PWGDQWRKMRRVLVAEAFNPSRVHWLLGKRNEEADNLVKFLYNQCSANQN------GAVV 195

Query: 203 NLRSITQHYSGNVMRRMMFDRRYYGGGRADGGPTAEEEEHIGALFTMLQHVYAFCVSDYM 262
           N+R   Q YSG++MR+M+F+R Y+G GR DGGP  EEEEH+ AL T+L + YAFCVSDY+
Sbjct: 196 NVRIAAQFYSGSIMRKMIFNRTYFGKGREDGGPGVEEEEHVSALLTLLTYAYAFCVSDYL 255

Query: 263 PCLKAFDLDGHERTVKKALNVIRKYEEPIIDERVQQWRDGRRTEAEDMLDIFISLKDGDG 322
           P L+ FD+DGHE+ V+KA+N+++K++EPI++ER+Q+WRDG+R E +D+LD+FISLKD +G
Sbjct: 256 PWLRVFDIDGHEKKVRKAMNIVKKHQEPIVNERLQEWRDGKRNEPDDLLDVFISLKDANG 315

Query: 323 RPLLSVREIKAQITELLLATVDNPSNAVEWAIAEMLNQPQILQTATEELDRVVGKQRLVQ 382
           +PLLS  EIKAQ TEL L+TVD+P N  EWA+ EMLNQP++L+ A EELDRVVGK+ LVQ
Sbjct: 316 QPLLSDEEIKAQTTELQLSTVDSPFNIAEWALTEMLNQPEMLKKAEEELDRVVGKKTLVQ 375

Query: 383 ESDLPNLKFLTACAREALRLHPIAPFNLPHIATADAVVAGYFIPAGSHVLLSRLGLGRNP 442
           ES +P+L ++ ACA+E +RLHP+ PFNLPH++ ADA VAGYFIP GS+V+LSRL LGRNP
Sbjct: 376 ESHVPHLPYIRACAKEVMRLHPVGPFNLPHVSIADAEVAGYFIPKGSNVILSRLELGRNP 435

Query: 443 RIWPDPMRFDPNRHLQ---DPGADPNLGEPDLRFITFTRGRRGCMGSALGTAITMMLLAR 502
           R+W +P+RF+P RHL    D   D  L E DLRF++F+ GRRGCMG  LG+ I +MLLAR
Sbjct: 436 RVWEEPLRFNPERHLNIAVDQQVD--LEENDLRFVSFSTGRRGCMGVGLGSTIVVMLLAR 495

Query: 503 LVQGFSWRLPPGAAEIDLSESDQLFLK--NPLFVFAEPRLLESLYP 544
           L+QGFSW LPP   +ID +E DQ++LK  +PL   A+PRL  S+YP
Sbjct: 496 LLQGFSWSLPPDVDKIDFTE-DQIYLKKASPLLAQAKPRLPASVYP 532

BLAST of Spg021805 vs. ExPASy Swiss-Prot
Match: Q6J540 (Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 1.2e-167
Identity = 282/531 (53.11%), Postives = 379/531 (71.37%), Query Frame = 0

Query: 20  SFMIFLTGSFMAAVLIWLFSYYGKRKQSVGTA---ALPPGPSPWPIVGCLPTIWRRKPRY 79
           SF +  + +F+  V+ +   +   +  SV  +    LPPGP PWPIVG LP +   +P  
Sbjct: 9   SFCLEFSWTFLLVVIFFFIIFKVTKSHSVNKSKKYKLPPGPKPWPIVGNLPEMLANRPAT 68

Query: 80  RWIHSVMEDLNTEIACIRLGKTHVIPVMSPELAVEFLKTHDSLFASRPITVTTDMFSDGF 139
            WIH +M+++NTEIACIRL  T VIPV  P +A EFLK HD+ FASRP  ++TD+ SDGF
Sbjct: 69  IWIHKLMKEMNTEIACIRLANTIVIPVTCPTIACEFLKKHDASFASRPKIMSTDIASDGF 128

Query: 140 LTAGVAPWGDQWRKMRRVLTSEILSPARHRWMLAKRTEEADNLLRYVFHLTTTGNDDDDV 199
           LT  + P+G+QW+KM+RVL + +LSP +H+W+L KR EEADNL+ Y+++      +D   
Sbjct: 129 LTTVLVPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNKCCKDVND--- 188

Query: 200 GGGAVVNLRSITQHYSGNVMRRMMFDRRYYGGGRADGGPTAEEEEHIGALFTMLQHVYAF 259
            G  +VN+R   QHY GNV R+++F+ RY+G    DGGP  EE EHI A FT+L++VYAF
Sbjct: 189 -GPGLVNIRIAAQHYGGNVFRKLIFNTRYFGKVMEDGGPGFEEVEHINATFTILKYVYAF 248

Query: 260 CVSDYMPCLKAFDLDGHERTVKKALNVIRKYEEPIIDERVQQWRDGRRTEAEDMLDIFIS 319
            +SD++P L+  DLDGH   + KA+ +++KY +PII +R++QW DG +T  ED+LD+ I 
Sbjct: 249 SISDFIPFLRRLDLDGHRSKIMKAMGIMKKYHDPIIHDRIKQWNDGLKTVEEDLLDVLIK 308

Query: 320 LKDGDGRPLLSVREIKAQITELLLATVDNPSNAVEWAIAEMLNQPQILQTATEELDRVVG 379
           LKD   +PLL+++EIKAQITEL +  VDNPSNA EWA+AEMLNQP++L+ ATEELD VVG
Sbjct: 309 LKDASNKPLLTLKEIKAQITELAIEMVDNPSNAFEWALAEMLNQPELLKRATEELDNVVG 368

Query: 380 KQRLVQESDLPNLKFLTACAREALRLHPIAPFNLPHIATADAVVAGYFIPAGSHVLLSRL 439
           K+RLVQESD+P L+F+ ACAREALRLHP+  FN+PH+   D +V  Y  P G+ VLLSR+
Sbjct: 369 KERLVQESDIPKLQFVKACAREALRLHPMEYFNVPHLCMNDTMVGDYLFPKGTQVLLSRV 428

Query: 440 GLGRNPRIWPDPMRFDPNRHLQDPGADPNLGEPDLRFITFTRGRRGCMGSALGTAITMML 499
            LGRNP+ W DP++F+P RHL++ G D  L EPDLRFI+FT GRR C G  LGT +T+ML
Sbjct: 429 ALGRNPKFWTDPLKFNPERHLKE-GIDVVLTEPDLRFISFTTGRRSCPGVTLGTTMTIML 488

Query: 500 LARLVQGFSWRLPPGAAEIDLSE-SDQLFLKNPLFVFAEPRLLESLYPDSE 547
            AR++ GFSW  PP  + IDL++ SD LF+  PL V A+PRL   LY  +E
Sbjct: 489 FARMLHGFSWSAPPNVSSIDLTQSSDDLFMAKPLCVVAKPRLAAELYSTNE 534

BLAST of Spg021805 vs. ExPASy Swiss-Prot
Match: Q6J541 (Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 3.7e-166
Identity = 287/537 (53.45%), Postives = 381/537 (70.95%), Query Frame = 0

Query: 12  TFLLAICKSFMIF-LTGSFMAAVLIWLFSYYGKRKQSVGTAALPPGPSPWPIVGCLPTIW 71
           TFLL +  SFMIF +T + +            K K+      LPPGP PWPIVG LP + 
Sbjct: 17  TFLLVVIFSFMIFKVTKTHLV----------NKSKK----YKLPPGPKPWPIVGNLPEML 76

Query: 72  RRKPRYRWIHSVMEDLNTEIACIRLGKTHVIPVMSPELAVEFLKTHDSLFASRPITVTTD 131
             +P   WIH +M+++NTEIACIRL  T VIPV  P +A EFLK HD+ FASRP  ++TD
Sbjct: 77  ANRPATIWIHKLMKEMNTEIACIRLANTIVIPVTCPTIACEFLKKHDASFASRPKIMSTD 136

Query: 132 MFSDGFLTAGVAPWGDQWRKMRRVLTSEILSPARHRWMLAKRTEEADNLLRYVFHLTTTG 191
           + SDGF+T  + P+G+QW+KM+RVL + +LSP +H+W+L KR EEADNL+ Y+++     
Sbjct: 137 IASDGFITTVLVPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNKCCKD 196

Query: 192 NDDDDVGGGAVVNLRSITQHYSGNVMRRMMFDRRYYGGGRADGGPTAEEEEHIGALFTML 251
            +D    G  +VN+R   QHY GNV R+++F+ RY+G    DGGP  EE EHI A FT+L
Sbjct: 197 VND----GPGLVNIRIAAQHYGGNVFRKLIFNSRYFGKVMEDGGPGFEEVEHINATFTIL 256

Query: 252 QHVYAFCVSDYMPCLKAFDLDGHERTVKKALNVIRKYEEPIIDERVQQWRDGRRTEAEDM 311
           ++VYAF +SD++P L+  DLDGH   + KA+ ++RKY +PIID+R++QW DG +T  ED+
Sbjct: 257 KYVYAFSISDFVPFLRRLDLDGHRSKIMKAMRIMRKYHDPIIDDRIKQWNDGLKTVEEDL 316

Query: 312 LDIFISLKDGDGRPLLSVREIKAQITELLLATVDNPSNAVEWAIAEMLNQPQILQTATEE 371
           LD+ I LKD + +PLL+++E+KAQI EL +  VDNPSNA EWA+AEM+NQP++L+ ATEE
Sbjct: 317 LDVLIKLKDANNKPLLTLKELKAQIIELAIEMVDNPSNAFEWALAEMINQPELLKRATEE 376

Query: 372 LDRVVGKQRLVQESDLPNLKFLTACAREALRLHPIAPFNLPHIATADAVVAGYFIPAGSH 431
           LD VVGK+RLVQESD+P L+F+ ACAREALRLHP+  FN+PH+   D +V  Y  P G+ 
Sbjct: 377 LDNVVGKERLVQESDIPKLQFVKACAREALRLHPMEYFNVPHLCMNDTMVGDYLFPKGTQ 436

Query: 432 VLLSRLGLGRNPRIWPDPMRFDPNRHLQDPGADPNLGEPDLRFITFTRGRRGCMGSALGT 491
           VLLSR+ LGRNP+ W DP++F+P RHL++ G D  L EPDLRFI+FT GRR C G ALGT
Sbjct: 437 VLLSRVALGRNPKFWTDPLKFNPERHLKE-GIDVVLTEPDLRFISFTTGRRSCPGVALGT 496

Query: 492 AITMMLLARLVQGFSWRLPPGAAEIDLSES-DQLFLKNPLFVFAEPRLLESLYPDSE 547
            +T+ML AR++ GFSW  PP  + IDL  S D LFL  PL + A+PRL   LY  +E
Sbjct: 497 TMTVMLFARMLHGFSWSPPPDVSSIDLVPSKDDLFLAKPLLLVAKPRLAAELYRTNE 534

BLAST of Spg021805 vs. ExPASy Swiss-Prot
Match: Q43135 (Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3)

HSP 1 Score: 585.5 bits (1508), Expect = 6.3e-166
Identity = 269/497 (54.12%), Postives = 361/497 (72.64%), Query Frame = 0

Query: 48  VGTAALPPGPSPWPIVGCLPTIWRRKPRYRWIHSVMEDLNTEIACIRLGKTHVIPVMSPE 107
           VG   LPPGP PWP+VG LP +   KP +RWIH +M ++ T+IAC++LG  HV+ +  PE
Sbjct: 63  VGNPPLPPGPVPWPVVGNLPEMLLNKPAFRWIHQMMREMGTDIACVKLGGVHVVSITCPE 122

Query: 108 LAVEFLKTHDSLFASRPITVTTDMFSDGFLTAGVAPWGDQWRKMRRVLTSEILSPARHRW 167
           +A E L+  D+ F SRP+T  ++ FS G+  A ++P+GDQW+KMRRVLTSEI+ P+RH W
Sbjct: 123 IAREVLRKQDANFISRPLTFASETFSGGYRNAVLSPYGDQWKKMRRVLTSEIICPSRHAW 182

Query: 168 MLAKRTEEADNLLRYVFHLTTTGNDDDDVGGGAVVNLRSITQHYSGNVMRRMMFDRRYYG 227
           +  KRT+EADNL RYV++L T         G   V++R + +HY GNV+RR+MF+RRY+G
Sbjct: 183 LHDKRTDEADNLTRYVYNLATKA-----ATGDVAVDVRHVARHYCGNVIRRLMFNRRYFG 242

Query: 228 GGRADGGPTAEEEEHIGALFTMLQHVYAFCVSDYMPCLKAFDLDGHERTVKKALNVIRKY 287
             +ADGGP   E  H+ A+FT L  +YAFCVSDY+P L+  DLDGHE+ VK+A   + + 
Sbjct: 243 EPQADGGPGPMEVLHMDAVFTSLGLLYAFCVSDYLPWLRGLDLDGHEKIVKEANVAVNRL 302

Query: 288 EEPIIDERVQQWRDGRRTEAEDMLDIFISLKDGDGRPLLSVREIKAQITELLLATVDNPS 347
            + +ID+R +QW+ G R E ED LD+ I+LKD  G PLL++ E+KAQ  ++  A VDNPS
Sbjct: 303 HDTVIDDRWRQWKSGERQEMEDFLDVLITLKDAQGNPLLTIEEVKAQSQDITFAAVDNPS 362

Query: 348 NAVEWAIAEMLNQPQILQTATEELDRVVGKQRLVQESDLPNLKFLTACAREALRLHPIAP 407
           NAVEWA+AEM+N P+++  A EELDRVVG++RLVQESD+P L ++ AC REA RLHP+AP
Sbjct: 363 NAVEWALAEMVNNPEVMAKAMEELDRVVGRERLVQESDIPKLNYVKACIREAFRLHPVAP 422

Query: 408 FNLPHIATADAVVAGYFIPAGSHVLLSRLGLGRNPRIWPDPMRFDPNRHLQDPGADPNLG 467
           FN+PH+A AD  +AGY +P GSHV+LSR GLGRNPR+W +P+RF P+RHL    +D  L 
Sbjct: 423 FNVPHVALADTTIAGYRVPKGSHVILSRTGLGRNPRVWDEPLRFYPDRHLATAASDVALT 482

Query: 468 EPDLRFITFTRGRRGCMGSALGTAITMMLLARLVQGFSWRLPPGAAEIDLSES-DQLFLK 527
           E DLRFI+F+ GRRGC+ ++LGTA+++ML  RL+QGF+W  P G   +DLSES    F+ 
Sbjct: 483 ENDLRFISFSTGRRGCIAASLGTAMSVMLFGRLLQGFTWSKPAGVEAVDLSESKSDTFMA 542

Query: 528 NPLFVFAEPRLLESLYP 544
            PL + AEPRL   LYP
Sbjct: 543 TPLVLHAEPRLPAHLYP 554

BLAST of Spg021805 vs. ExPASy Swiss-Prot
Match: Q9M7B8 (Valine N-monooxygenase 1 OS=Manihot esculenta OX=3983 GN=CYP79D1 PE=1 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 3.5e-164
Identity = 271/494 (54.86%), Postives = 365/494 (73.89%), Query Frame = 0

Query: 53  LPPGPSPWPIVGCLPTIWRRKPRYRWIHSVMEDLNTEIACIRLGKTHVIPVMSPELAVEF 112
           LPPGP+PWP++G +P + R +P +RWIH +M+D+NT+I  IR G+T+ +P+  P LA E 
Sbjct: 59  LPPGPTPWPLIGNIPEMIRYRPTFRWIHQLMKDMNTDICLIRFGRTNFVPISCPVLAREI 118

Query: 113 LKTHDSLFASRPITVTTDMFSDGFLTAGVAPWGDQWRKMRRVLTSEILSPARHRWMLAKR 172
           LK +D++F++RP T++    S G+LT  V P+ DQW+KMR++LTSEI+SPARH+W+  KR
Sbjct: 119 LKKNDAIFSNRPKTLSAKSMSGGYLTTIVVPYNDQWKKMRKILTSEIISPARHKWLHDKR 178

Query: 173 TEEADNLLRYVFHLTTTGNDDDDVGGGAVVNLRSITQHYSGNVMRRMMFDRRYYGGGRAD 232
            EEADNL+ Y+ H     N +        VNLR+ T+HY GNV+R+M+F +RY+G G  D
Sbjct: 179 AEEADNLVFYI-HNQFKANKN--------VNLRTATRHYGGNVIRKMVFSKRYFGKGMPD 238

Query: 233 GGPTAEEEEHIGALFTMLQHVYAFCVSDYMPCLKAFDLDGHERTVKKALNVIRKYEEPII 292
           GGP  EE EHI A+FT L+++Y FC+SD++P L   DLDG E+ V  A   IR Y+ P+I
Sbjct: 239 GGPGPEEIEHIDAVFTALKYLYGFCISDFLPFLLGLDLDGQEKFVLDANKTIRDYQNPLI 298

Query: 293 DERVQQWRDGRRTEAEDMLDIFISLKDGDGRPLLSVREIKAQITELLLATVDNPSNAVEW 352
           DER+QQW+ G R E ED+LD+FI+LKD DG PLL+  EIK QI E+++ATVDNPSNA+EW
Sbjct: 299 DERIQQWKSGERKEMEDLLDVFITLKDSDGNPLLTPDEIKNQIAEIMIATVDNPSNAIEW 358

Query: 353 AIAEMLNQPQILQTATEELDRVVGKQRLVQESDLPNLKFLTACAREALRLHPIAPFNLPH 412
           A+ EMLNQP+IL+ ATEELDRVVGK RLVQESD+PNL ++ ACAREA RLHP+A FN+PH
Sbjct: 359 AMGEMLNQPEILKKATEELDRVVGKDRLVQESDIPNLDYVKACAREAFRLHPVAHFNVPH 418

Query: 413 IATADAVVAGYFIPAGSHVLLSRLGLGRNPRIWPDPMRFDPNRHLQDPGADPNLGEPDLR 472
           +A  D V+  YFIP GS  +LSR GLGRNP+ W DP+++DP RH+ +   +  L E +LR
Sbjct: 419 VAMEDTVIGDYFIPKGSWAVLSRYGLGRNPKTWSDPLKYDPERHMNE--GEVVLTEHELR 478

Query: 473 FITFTRGRRGCMGSALGTAITMMLLARLVQGFSWRLPPGAAEIDLSES-DQLFLKNPLFV 532
           F+TF+ GRRGC+ S LG+ +T MLLAR++Q F+W  P   ++IDL+E+ D+L    P+  
Sbjct: 479 FVTFSTGRRGCVASLLGSCMTTMLLARMLQCFTWTPPANVSKIDLAETLDELTPATPISA 538

Query: 533 FAEPRLLESLYPDS 546
           FA+PRL   LYP S
Sbjct: 539 FAKPRLAPHLYPTS 541

BLAST of Spg021805 vs. ExPASy TrEMBL
Match: A0A5D3DVB8 (Tyrosine N-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold225G00870 PE=3 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 4.0e-256
Identity = 448/549 (81.60%), Postives = 477/549 (86.89%), Query Frame = 0

Query: 8   LSSSTFLLAICKSFMIFLTGSFMAAVLIWLFSYYGKR--KQSVGT--AALPPGPSPWPIV 67
           LSSST        FMI   GS M    I    +YGKR  K  VG+  AALPPGPSPWPIV
Sbjct: 7   LSSST--------FMILFYGSIMVVAFI---LFYGKRRNKYCVGSTAAALPPGPSPWPIV 66

Query: 68  GCLPTIWRRKPRYRWIHSVMEDLNTEIACIRLGKTHVIPVMSPELAVEFLKTHDSLFASR 127
           GCLPT+WR+KPRYRW+H +ME+LNTEIACIR+GK HVIPV SPELA+EFLKTHDS+FASR
Sbjct: 67  GCLPTLWRKKPRYRWVHKIMEELNTEIACIRIGKIHVIPVTSPELAMEFLKTHDSVFASR 126

Query: 128 PITVTTDMFSDGFLTAGVAPWGDQWRKMRRVLTSEILSPARHRWMLAKRTEEADNLLRYV 187
           PITVTTDMFSDGFLTAGVAPWG+QW+KMRR+LTSEILSPARHRWML+KRTEEADNLLRYV
Sbjct: 127 PITVTTDMFSDGFLTAGVAPWGNQWKKMRRILTSEILSPARHRWMLSKRTEEADNLLRYV 186

Query: 188 FHLTTTGNDDDDVGGGAVVNLRSITQHYSGNVMRRMMFDRRYYGGGRADGGPTAEEEEHI 247
           F LT T            V++RSITQHYSGNVMRRMMF+RRY+G GRADGGP  EEEEHI
Sbjct: 187 FSLTKTTPTS--------VSVRSITQHYSGNVMRRMMFNRRYFGKGRADGGPATEEEEHI 246

Query: 248 GALFTMLQHVYAFCVSDYMPCLKAFDLDGHERTVKKALNVIRKYEEPIIDERVQQWRDGR 307
           GALFTMLQHVYAFCVSDYM CLKAFDLDGHER VKKALNVIRKYEEPIIDERVQQWRDG+
Sbjct: 247 GALFTMLQHVYAFCVSDYMSCLKAFDLDGHERIVKKALNVIRKYEEPIIDERVQQWRDGK 306

Query: 308 RTEAEDMLDIFISLKDGDGRPLLSVREIKAQITELLLATVDNPSNAVEWAIAEMLNQPQI 367
           R EAEDMLDIFISLKD  G PLLSV+EIKAQITELLLATVDNPSNAVEWA+AEMLNQPQ 
Sbjct: 307 RIEAEDMLDIFISLKDESGEPLLSVKEIKAQITELLLATVDNPSNAVEWAMAEMLNQPQH 366

Query: 368 LQTATEELDRVVGKQRLVQESDLPNLKFLTACAREALRLHPIAPFNLPHIATADAVVAGY 427
           LQ ATEELDRVVGK+RLVQESD+PNLKFLTACAREALRLHPIAPFNLPHIAT D VVAGY
Sbjct: 367 LQQATEELDRVVGKERLVQESDIPNLKFLTACAREALRLHPIAPFNLPHIATTDVVVAGY 426

Query: 428 FIPAGSHVLLSRLGLGRNPRIWPDPMRFDPNRHLQDPGADPNLGEPDLRFITFTRGRRGC 487
            IPAGSHVLLSRLGLGRN RIWPDPMRFDPNRHLQDPGAD  L EPDLRFITFTRGRRGC
Sbjct: 427 LIPAGSHVLLSRLGLGRNHRIWPDPMRFDPNRHLQDPGADLGLAEPDLRFITFTRGRRGC 486

Query: 488 MGSALGTAITMMLLARLVQGFSWRLPPGAAEIDLSESDQLFLKNPLFVFAEPRLLESLYP 547
           MG  LGTAITMMLLARLVQGF+W+LPPG A I+LSESDQLFLK+PLF  A+PRL ESLYP
Sbjct: 487 MGGTLGTAITMMLLARLVQGFTWQLPPGVAAIELSESDQLFLKDPLFALAQPRLPESLYP 536

Query: 548 DSETGEKLF 553
           D E  +K F
Sbjct: 547 DFEIEKKDF 536

BLAST of Spg021805 vs. ExPASy TrEMBL
Match: A0A5A7UJK2 (Tyrosine N-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold319G002190 PE=3 SV=1)

HSP 1 Score: 889.4 bits (2297), Expect = 7.6e-255
Identity = 446/549 (81.24%), Postives = 476/549 (86.70%), Query Frame = 0

Query: 8   LSSSTFLLAICKSFMIFLTGSFMAAVLIWLFSYYGKR--KQSVGT--AALPPGPSPWPIV 67
           LSSST        FMI   GS M    I    +YGKR  K  VG+  AALPPGP PWPIV
Sbjct: 7   LSSST--------FMILFYGSIMVVAFI---LFYGKRRNKYCVGSTAAALPPGPIPWPIV 66

Query: 68  GCLPTIWRRKPRYRWIHSVMEDLNTEIACIRLGKTHVIPVMSPELAVEFLKTHDSLFASR 127
           GCLPT+WR+KPRYRW+H +ME+LNTEIACIR+GK HVIPV SPELA+EFLKTHDS+FASR
Sbjct: 67  GCLPTLWRKKPRYRWVHKIMEELNTEIACIRIGKIHVIPVTSPELAMEFLKTHDSVFASR 126

Query: 128 PITVTTDMFSDGFLTAGVAPWGDQWRKMRRVLTSEILSPARHRWMLAKRTEEADNLLRYV 187
           PITVTTDMFSDGFLTAGVAPWG+QW+KMRR+LTSEILSPARHRWML+KRTEEADNLLRYV
Sbjct: 127 PITVTTDMFSDGFLTAGVAPWGNQWKKMRRILTSEILSPARHRWMLSKRTEEADNLLRYV 186

Query: 188 FHLTTTGNDDDDVGGGAVVNLRSITQHYSGNVMRRMMFDRRYYGGGRADGGPTAEEEEHI 247
           F LT T            V++RSITQHYSGNVMRRMMF+RRY+G GRADGGP  EEEEHI
Sbjct: 187 FSLTKTTPTS--------VSVRSITQHYSGNVMRRMMFNRRYFGKGRADGGPATEEEEHI 246

Query: 248 GALFTMLQHVYAFCVSDYMPCLKAFDLDGHERTVKKALNVIRKYEEPIIDERVQQWRDGR 307
           GALFTMLQHVYAFCVSDYM CLKAFDLDGHER VKKALNVIRKYEEPIIDERVQQWRDG+
Sbjct: 247 GALFTMLQHVYAFCVSDYMSCLKAFDLDGHERIVKKALNVIRKYEEPIIDERVQQWRDGK 306

Query: 308 RTEAEDMLDIFISLKDGDGRPLLSVREIKAQITELLLATVDNPSNAVEWAIAEMLNQPQI 367
           R EAEDMLDIFISLKD  G PLLSV+EIKAQITELLLATVDNPSNAVEWA+AEMLNQPQ 
Sbjct: 307 RIEAEDMLDIFISLKDESGEPLLSVKEIKAQITELLLATVDNPSNAVEWAMAEMLNQPQH 366

Query: 368 LQTATEELDRVVGKQRLVQESDLPNLKFLTACAREALRLHPIAPFNLPHIATADAVVAGY 427
           LQ ATEELDRVVGK+RLVQESD+PNLKFLTACAREALRLHPIAPFNLPHIAT D VVAGY
Sbjct: 367 LQQATEELDRVVGKERLVQESDIPNLKFLTACAREALRLHPIAPFNLPHIATTDVVVAGY 426

Query: 428 FIPAGSHVLLSRLGLGRNPRIWPDPMRFDPNRHLQDPGADPNLGEPDLRFITFTRGRRGC 487
            IPAGSHVLLSRLGLGRN RIWPDPMRFDPNRHLQDPGAD  L EPDLRFITFTRGRRGC
Sbjct: 427 LIPAGSHVLLSRLGLGRNHRIWPDPMRFDPNRHLQDPGADLGLAEPDLRFITFTRGRRGC 486

Query: 488 MGSALGTAITMMLLARLVQGFSWRLPPGAAEIDLSESDQLFLKNPLFVFAEPRLLESLYP 547
           MG  LGTAITMMLLARLVQGF+W+LPPG A I+LSESDQLFLK+PLF  A+PRL ESLYP
Sbjct: 487 MGGTLGTAITMMLLARLVQGFTWQLPPGVAAIELSESDQLFLKDPLFALAQPRLPESLYP 536

Query: 548 DSETGEKLF 553
           + E  +K F
Sbjct: 547 NFEIEKKDF 536

BLAST of Spg021805 vs. ExPASy TrEMBL
Match: A0A1S3BPC7 (tyrosine N-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103492071 PE=3 SV=1)

HSP 1 Score: 886.3 bits (2289), Expect = 6.4e-254
Identity = 434/517 (83.95%), Postives = 464/517 (89.75%), Query Frame = 0

Query: 40  YYGKR--KQSVGT--AALPPGPSPWPIVGCLPTIWRRKPRYRWIHSVMEDLNTEIACIRL 99
           +YGKR  K  VG+  AALPPGP PWPIVGCLPT+WR+KPRYRW+H +ME+LNTEIACIR+
Sbjct: 8   FYGKRRNKYCVGSTAAALPPGPIPWPIVGCLPTLWRKKPRYRWVHKIMEELNTEIACIRI 67

Query: 100 GKTHVIPVMSPELAVEFLKTHDSLFASRPITVTTDMFSDGFLTAGVAPWGDQWRKMRRVL 159
           GK HVIPV SPELA+EFLKTHDS+FASRPITVTTDMFSDGFLTAGVAPWG+QW+KMRR+L
Sbjct: 68  GKIHVIPVTSPELAMEFLKTHDSVFASRPITVTTDMFSDGFLTAGVAPWGNQWKKMRRIL 127

Query: 160 TSEILSPARHRWMLAKRTEEADNLLRYVFHLTTTGNDDDDVGGGAVVNLRSITQHYSGNV 219
           TSEILSPARHRWML+KRTEEADNLLRYVF LT T            V++RSITQHYSGNV
Sbjct: 128 TSEILSPARHRWMLSKRTEEADNLLRYVFSLTKTTPTS--------VSVRSITQHYSGNV 187

Query: 220 MRRMMFDRRYYGGGRADGGPTAEEEEHIGALFTMLQHVYAFCVSDYMPCLKAFDLDGHER 279
           MRRMMF+RRY+G GRADGGP  EEEEHIGALFTMLQHVYAFCVSDYM CLKAFDLDGHER
Sbjct: 188 MRRMMFNRRYFGKGRADGGPATEEEEHIGALFTMLQHVYAFCVSDYMSCLKAFDLDGHER 247

Query: 280 TVKKALNVIRKYEEPIIDERVQQWRDGRRTEAEDMLDIFISLKDGDGRPLLSVREIKAQI 339
            VKKALNVIRKYEEPIIDERVQQWRDG+R EAEDMLDIFISLKD  G PLLSV+EIKAQI
Sbjct: 248 IVKKALNVIRKYEEPIIDERVQQWRDGKRIEAEDMLDIFISLKDESGEPLLSVKEIKAQI 307

Query: 340 TELLLATVDNPSNAVEWAIAEMLNQPQILQTATEELDRVVGKQRLVQESDLPNLKFLTAC 399
           TELLLATVDNPSNAVEWA+AEMLNQPQ LQ ATEELDRVVGK+RLVQESD+PNLKFLTAC
Sbjct: 308 TELLLATVDNPSNAVEWAMAEMLNQPQHLQQATEELDRVVGKERLVQESDIPNLKFLTAC 367

Query: 400 AREALRLHPIAPFNLPHIATADAVVAGYFIPAGSHVLLSRLGLGRNPRIWPDPMRFDPNR 459
           AREALRLHPIAPFNLPHIAT D VVAGY IPAGSHVLLSRLGLGRN RIWPDPMRFDPNR
Sbjct: 368 AREALRLHPIAPFNLPHIATTDVVVAGYLIPAGSHVLLSRLGLGRNHRIWPDPMRFDPNR 427

Query: 460 HLQDPGADPNLGEPDLRFITFTRGRRGCMGSALGTAITMMLLARLVQGFSWRLPPGAAEI 519
           HLQDPGAD  L EPDLRFITFTRGRRGCMG  LGTAITMMLLARLVQGF+W+LPPG A I
Sbjct: 428 HLQDPGADLGLAEPDLRFITFTRGRRGCMGGTLGTAITMMLLARLVQGFTWQLPPGVAAI 487

Query: 520 DLSESDQLFLKNPLFVFAEPRLLESLYPDSETGEKLF 553
           +LSESDQLFLK+PLF  A+PRL ESLYP+ E  +K F
Sbjct: 488 ELSESDQLFLKDPLFALAQPRLPESLYPNFEIEKKDF 516

BLAST of Spg021805 vs. ExPASy TrEMBL
Match: A0A7N2KLH4 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 712.2 bits (1837), Expect = 1.7e-201
Identity = 335/523 (64.05%), Postives = 420/523 (80.31%), Query Frame = 0

Query: 21  FMIFLTGSFMAAVLIWLFSYYGKRKQSVGTAALPPGPSPWPIVGCLPTIWRRKPRYRWIH 80
           ++ FL     A +  +L   + K K+ +    LPPGP PWPIVG LP +WR+KPRYRW+H
Sbjct: 24  YVTFLFALLFAFIFFFLSKIFTKSKRPL----LPPGPKPWPIVGNLPEMWRKKPRYRWLH 83

Query: 81  SVMEDLNTEIACIRLGKTHVIPVMSPELAVEFLKTHDSLFASRPITVTTDMFSDGFLTAG 140
           S+M++LNTEIAC+RLG  +V+PV SPE+A+EFL+ HD++ ASRPITVTT MFSDGFLTA 
Sbjct: 84  SLMKELNTEIACVRLGSVYVVPVTSPEIAMEFLRQHDAVMASRPITVTTSMFSDGFLTAV 143

Query: 141 VAPWGDQWRKMRRVLTSEILSPARHRWMLAKRTEEADNLLRYVFHLTTTGNDDDDVGGGA 200
           VAPWG QW+KMR+VLT EIL+P R  W+ +KR EEADNLL+ V ++  +         G+
Sbjct: 144 VAPWGKQWKKMRKVLTHEILNPTRLEWLRSKRKEEADNLLQVVNNICKS---------GS 203

Query: 201 VVNLRSITQHYSGNVMRRMMFDRRYYGGGRADGGPTAEEEEHIGALFTMLQHVYAFCVSD 260
           VV++R +++H++GN++RRMMF++RYY  GR DGGP  EE+EHI ALFT+L +VYA CVSD
Sbjct: 204 VVDVRLVSRHFTGNILRRMMFNKRYYRQGREDGGPCVEEQEHIKALFTVLLYVYALCVSD 263

Query: 261 YMPCLKAFDLDGHERTVKKALNVIRKYEEPIIDERVQQWRDGRRTEAEDMLDIFISLKDG 320
           Y+P LK  DLDGHE+TV+ ALNVI+KYE+PII+ERV+ W++G+RT+ ED+LD+FIS+KD 
Sbjct: 264 YLPILKPLDLDGHEKTVRNALNVIKKYEDPIINERVEMWKEGKRTQPEDLLDVFISVKDD 323

Query: 321 DGRPLLSVREIKAQITELLLATVDNPSNAVEWAIAEMLNQPQILQTATEELDRVVGKQRL 380
           +G+PLLSV EIKAQITELLLAT+DNPSN  EWA+AEMLNQP+ LQ A EELDRVVGK+RL
Sbjct: 324 NGKPLLSVAEIKAQITELLLATLDNPSNIAEWALAEMLNQPEQLQKAVEELDRVVGKERL 383

Query: 381 VQESDLPNLKFLTACAREALRLHPIAPFNLPHIATADAVVAGYFIPAGSHVLLSRLGLGR 440
           V+ESD+PNL ++ ACARE+LRLHPIAPFNLPH+A  D  VAGYFIP GS VLLSRLGLGR
Sbjct: 384 VEESDIPNLSYVVACARESLRLHPIAPFNLPHVAKEDLTVAGYFIPKGSQVLLSRLGLGR 443

Query: 441 NPRIWPDPMRFDPNRHLQDPGADPNLGEPDLRFITFTRGRRGCMGSALGTAITMMLLARL 500
           NPR+W DP+RF+P RH +D   +  L EP LRFI+FTRGRRGCMG  LGT IT+ML ARL
Sbjct: 444 NPRVWEDPLRFNPERHFKDNSHELGLAEPGLRFISFTRGRRGCMGGTLGTLITVMLFARL 503

Query: 501 VQGFSWRLPPGAAEIDLSESDQLFLKNPLFVFAEPRLLESLYP 544
           +QGF+WR+PP   +IDL E+D+LFL  PL V+AEPRL   LYP
Sbjct: 504 LQGFTWRIPPELEKIDLKENDRLFLAKPLHVYAEPRLPAHLYP 533

BLAST of Spg021805 vs. ExPASy TrEMBL
Match: A0A6J1D6G9 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498 PE=3 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 9.7e-194
Identity = 337/532 (63.35%), Postives = 418/532 (78.57%), Query Frame = 0

Query: 16  AICKSFMIFLTGSFMAAVLIWLFSYYGKRKQSVGTAALPPGPSPWPIVGCLPTIWRRK-P 75
           AI K+F +F+  +F++A+       Y KRK SV    LPPGP+PWP+VGCLP +   K P
Sbjct: 18  AITKTFTLFVFMAFISAI---FSQCYRKRKHSV--LQLPPGPNPWPLVGCLPAMLATKSP 77

Query: 76  RYRWIHSVMEDLNTEIACIRLGKTHVIPVMSPELAVEFLKTHDSLFASR-PITVTTDMFS 135
           +Y+WIH+VM+  +TEIACIRLG T+V+PV SPELAVEFLK HDS+FASR  I+ T ++ S
Sbjct: 78  KYKWIHAVMKQFDTEIACIRLGNTYVMPVTSPELAVEFLKRHDSVFASRSTISNTVNVLS 137

Query: 136 DGFLTAGVAPWGDQWRKMRRVLTSEILSPARHRWMLAKRTEEADNLLRYVFHLTTTGNDD 195
            G LT   +P G QW+KMRR+L SEI  P+    ML  RT EAD LLRY+F LTTT   +
Sbjct: 138 RGLLTTAFSPLGHQWKKMRRILASEIFCPSTLHQMLGHRTNEADTLLRYIFSLTTTNTVN 197

Query: 196 DDVGGGAVVNLRSITQHYSGNVMRRMMFDRRYYGGGRADGGPTAEEEEHIGALFTMLQHV 255
            D   GAVVN+RS+TQHY GN++RRM+F++RYYG GR DGGP+ EEEEH  AL T+L+HV
Sbjct: 198 KD---GAVVNVRSVTQHYCGNIIRRMLFNKRYYGKGREDGGPSFEEEEHNQALLTILRHV 257

Query: 256 YAFCVSDYMPCLKAFDLDGHERTVKKALNVIRKYEEPIIDERVQQWRDGRRTEAEDMLDI 315
            AF +SD+MPCLK FDLDGHE+ +K+AL  + KY+EPII+ERVQQW+DG+R  AED+LDI
Sbjct: 258 NAFSISDFMPCLKPFDLDGHEKIMKRALKALWKYDEPIINERVQQWKDGKREGAEDILDI 317

Query: 316 FISLKDGDGRPLLSVREIKAQITELLLATVDNPSNAVEWAIAEMLNQPQILQTATEELDR 375
            IS KDG+G+ LL++ EIKAQITEL +AT+DNPSNAVEW +AE+LNQP+ILQ A EELDR
Sbjct: 318 LISTKDGNGKSLLTIDEIKAQITELQIATIDNPSNAVEWTMAELLNQPKILQKAIEELDR 377

Query: 376 VVGKQRLVQESDLPNLKFLTACAREALRLHPIAPFNLPHIATADAVVAGYFIPAGSHVLL 435
           VVG++RLVQESD+P LK+LTACARE+ RLHP +PFN+PH++ AD VVAGYFIP GS V+L
Sbjct: 378 VVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNIPHVSNADIVVAGYFIPKGSEVIL 437

Query: 436 SRLGLGRNPRIWPDPMRFDPNRHLQDPGADPNLGEPDLRFITFTRGRRGCMGSALGTAIT 495
           SR GLGRNPRIW DPMRFDP RHL+D   +  L EP+LRFI+FTRGRRGC+GS+LGT IT
Sbjct: 438 SRSGLGRNPRIWEDPMRFDPERHLKDETVELGLSEPNLRFISFTRGRRGCVGSSLGTNIT 497

Query: 496 MMLLARLVQGFSWRLPPGAAEIDLSESDQLFLKNPLFVFAEPRLLESLYPDS 546
           MMLL RL+QGFSW LP G  +I+ SE+D+L L  PL + A+PRL   +YP S
Sbjct: 498 MMLLGRLLQGFSWSLPFGTTKIEFSEADELSLPKPLHLHAKPRLPHIMYPTS 541

BLAST of Spg021805 vs. TAIR 10
Match: AT5G05260.1 (cytochrome p450 79a2 )

HSP 1 Score: 573.2 bits (1476), Expect = 2.3e-163
Identity = 278/495 (56.16%), Postives = 360/495 (72.73%), Query Frame = 0

Query: 52  ALPPGPSPWPIVGCLPTI-WRRKPRYRWIHSVMEDLNTEIACIRLGKTHVIPVMSPELAV 111
           +LPPGP  WP++G LP I  R KP +RWIHS+M++LNT+IACIRL  THVIPV SP +A 
Sbjct: 33  SLPPGPKSWPLIGNLPEILGRNKPVFRWIHSLMKELNTDIACIRLANTHVIPVTSPRIAR 92

Query: 112 EFLKTHDSLFASRPITVTTDMFSDGFLTAGVAPWGDQWRKMRRVLTSEILSPARHRWMLA 171
           E LK  DS+FA+RP+T+ T+  S G+LT  V P G+QW+KMRRV+ S + S    + ML 
Sbjct: 93  EILKKQDSVFATRPLTMGTEYCSRGYLTVAVEPQGEQWKKMRRVVASHVTSKKSFQMMLQ 152

Query: 172 KRTEEADNLLRYVFHLTTTGNDDDDVGGGAVVNLRSITQHYSGNVMRRMMFDRRYYGGGR 231
           KRTEEADNL+RY+ + +     +  V    V++LR   + YSGNV R+MMF  R++G G 
Sbjct: 153 KRTEEADNLVRYINNRSVKNRGNAFV----VIDLRLAVRQYSGNVARKMMFGIRHFGKGS 212

Query: 232 ADG-GPTAEEEEHIGALFTMLQHVYAFCVSDYMPCLKAFDLDGHERTVKKALNVIRKYEE 291
            DG GP  EE EH+ +LFT+L H+YAF +SDY+P L+  DL+GHE+ V  A+  + KY +
Sbjct: 213 EDGSGPGLEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVSNAMRNVSKYND 272

Query: 292 PIIDERVQQWRDGRRTEAEDMLDIFISLKDGDGRPLLSVREIKAQITELLLATVDNPSNA 351
           P +DER+ QWR+G+  E +D LD+FI  KD DG+P LS  EIKAQ+TEL+LATVDNPSNA
Sbjct: 273 PFVDERLMQWRNGKMKEPQDFLDMFIIAKDTDGKPTLSDEEIKAQVTELMLATVDNPSNA 332

Query: 352 VEWAIAEMLNQPQILQTATEELDRVVGKQRLVQESDLPNLKFLTACAREALRLHPIAPFN 411
            EW +AEM+N+P I+Q A EE+DRVVGK RLV ESDLPNL ++ AC +EA RLHP+APFN
Sbjct: 333 AEWGMAEMINEPSIMQKAVEEIDRVVGKDRLVIESDLPNLNYVKACVKEAFRLHPVAPFN 392

Query: 412 LPHIATADAVVAGYFIPAGSHVLLSRLGLGRNPRIWPDPMRFDPNRHLQDPGADPNLGEP 471
           LPH++T D VV GYFIP GSHVL+SR+G+GRNP +W  P +FDP RHL       +L E 
Sbjct: 393 LPHMSTTDTVVDGYFIPKGSHVLISRMGIGRNPSVWDKPHKFDPERHL-STNTCVDLNES 452

Query: 472 DLRFITFTRGRRGCMGSALGTAITMMLLARLVQGFSWRLPPGAAEIDLSES-DQLFLKNP 531
           DL  I+F+ GRRGCMG  +G+A+T MLLARL+QGF+W   PG  +ID+SES + LF+  P
Sbjct: 453 DLNIISFSAGRRGCMGVDIGSAMTYMLLARLIQGFTWLPVPGKNKIDISESKNDLFMAKP 512

Query: 532 LFVFAEPRLLESLYP 544
           L+  A PRL   +YP
Sbjct: 513 LYAVATPRLAPHVYP 522

BLAST of Spg021805 vs. TAIR 10
Match: AT4G39950.1 (cytochrome P450, family 79, subfamily B, polypeptide 2 )

HSP 1 Score: 552.0 bits (1421), Expect = 5.5e-157
Identity = 259/493 (52.54%), Postives = 358/493 (72.62%), Query Frame = 0

Query: 53  LPPGPSPWPIVGCLPTIWRRKPRYRWIHSVMEDLNTEIACIRLGKTHVIPVMSPELAVEF 112
           LPPGP+ WPI+G +PT+ + +P +RW+HS+M+ LNTEIAC++LG THVI V  P++A E 
Sbjct: 56  LPPGPTGWPIIGMIPTMLKSRPVFRWLHSIMKQLNTEIACVKLGNTHVITVTCPKIAREI 115

Query: 113 LKTHDSLFASRPITVTTDMFSDGFLTAGVAPWGDQWRKMRRVLTSEILSPARHRWMLAKR 172
           LK  D+LFASRP+T    + S+G+ T  + P+GDQ++KMR+V+ +E++ PARHRW+  KR
Sbjct: 116 LKQQDALFASRPLTYAQKILSNGYKTCVITPFGDQFKKMRKVVMTELVCPARHRWLHQKR 175

Query: 173 TEEADNLLRYVFHLTTTGNDDDDVGGGAVVNLRSITQHYSGNVMRRMMFDRRYYGGGRA- 232
           +EE D+L  +V+++         V     V+ R +T+HY GN ++++MF  R +    A 
Sbjct: 176 SEENDHLTAWVYNM---------VKNSGSVDFRFMTRHYCGNAIKKLMFGTRTFSKNTAP 235

Query: 233 DGGPTAEEEEHIGALFTMLQHVYAFCVSDYMPCLKAFDLDGHERTVKKALNVIRKYEEPI 292
           DGGPT E+ EH+ A+F  L   +AFC+SDY+P L   DL+GHE+ ++++  ++ KY +PI
Sbjct: 236 DGGPTVEDVEHMEAMFEALGFTFAFCISDYLPMLTGLDLNGHEKIMRESSAIMDKYHDPI 295

Query: 293 IDERVQQWRDGRRTEAEDMLDIFISLKDGDGRPLLSVREIKAQITELLLATVDNPSNAVE 352
           IDER++ WR+G+RT+ ED LDIFIS+KD  G PLL+  EIK  I EL++A  DNPSNAVE
Sbjct: 296 IDERIKMWREGKRTQIEDFLDIFISIKDEQGNPLLTADEIKPTIKELVMAAPDNPSNAVE 355

Query: 353 WAIAEMLNQPQILQTATEELDRVVGKQRLVQESDLPNLKFLTACAREALRLHPIAPFNLP 412
           WA+AEM+N+P+IL+ A EE+DRVVGK+RLVQESD+P L ++ A  REA RLHP+A FNLP
Sbjct: 356 WAMAEMVNKPEILRKAMEEIDRVVGKERLVQESDIPKLNYVKAILREAFRLHPVAAFNLP 415

Query: 413 HIATADAVVAGYFIPAGSHVLLSRLGLGRNPRIWPDPMRFDPNRHLQDPGADPNLGEPDL 472
           H+A +D  VAGY IP GS VLLSR GLGRNP++W DP+ F P RHL +  ++  L E DL
Sbjct: 416 HVALSDTTVAGYHIPKGSQVLLSRYGLGRNPKVWADPLCFKPERHLNE-CSEVTLTENDL 475

Query: 473 RFITFTRGRRGCMGSALGTAITMMLLARLVQGFSWRLPPGAAEIDLSESD-QLFLKNPLF 532
           RFI+F+ G+RGC   ALGTA+T M+LARL+QGF+W+LP     ++L ES   +FL  PL 
Sbjct: 476 RFISFSTGKRGCAAPALGTALTTMMLARLLQGFTWKLPENETRVELMESSHDMFLAKPLV 535

Query: 533 VFAEPRLLESLYP 544
           +  + RL E LYP
Sbjct: 536 MVGDLRLPEHLYP 538

BLAST of Spg021805 vs. TAIR 10
Match: AT2G22330.1 (cytochrome P450, family 79, subfamily B, polypeptide 3 )

HSP 1 Score: 542.7 bits (1397), Expect = 3.3e-154
Identity = 265/543 (48.80%), Postives = 371/543 (68.32%), Query Frame = 0

Query: 3   ASALALSSSTFLLAICKSFMIFLTGSFMAAVLIWLFSYYGKRKQSVGTAALPPGPSPWPI 62
           +S L   SS  + +    +++    +  A   + + +      ++     LPPGP+ +PI
Sbjct: 8   SSDLTTKSSLGMSSFTNMYLLTTLQALAALCFLMILNKIKSSSRNKKLHPLPPGPTGFPI 67

Query: 63  VGCLPTIWRRKPRYRWIHSVMEDLNTEIACIRLGKTHVIPVMSPELAVEFLKTHDSLFAS 122
           VG +P + + +P +RW+HS+M++LNTEIAC+RLG THVIPV  P++A E  K  D+LFAS
Sbjct: 68  VGMIPAMLKNRPVFRWLHSLMKELNTEIACVRLGNTHVIPVTCPKIAREIFKQQDALFAS 127

Query: 123 RPITVTTDMFSDGFLTAGVAPWGDQWRKMRRVLTSEILSPARHRWMLAKRTEEADNLLRY 182
           RP+T    + S+G+ T  + P+G+Q++KMR+V+ +EI+ PARHRW+   R EE D+L  +
Sbjct: 128 RPLTYAQKILSNGYKTCVITPFGEQFKKMRKVIMTEIVCPARHRWLHDNRAEETDHLTAW 187

Query: 183 VFHLTTTGNDDDDVGGGAVVNLRSITQHYSGNVMRRMMFDRRYYG-GGRADGGPTAEEEE 242
           ++++         V     V+LR +T+HY GN ++R+MF  R +     ADGGPT E+ E
Sbjct: 188 LYNM---------VKNSEPVDLRFVTRHYCGNAIKRLMFGTRTFSEKTEADGGPTLEDIE 247

Query: 243 HIGALFTMLQHVYAFCVSDYMPCLKAFDLDGHERTVKKALNVIRKYEEPIIDERVQQWRD 302
           H+ A+F  L   +AFC+SDY+P L   DL+GHE+ ++++  ++ KY +PIIDER++ WR+
Sbjct: 248 HMDAMFEGLGFTFAFCISDYLPMLTGLDLNGHEKIMRESSAIMDKYHDPIIDERIKMWRE 307

Query: 303 GRRTEAEDMLDIFISLKDGDGRPLLSVREIKAQITELLLATVDNPSNAVEWAIAEMLNQP 362
           G+RT+ ED LDIFIS+KD  G+PLL+  EIK  I EL++A  DNPSNAVEWAIAEM+N+P
Sbjct: 308 GKRTQIEDFLDIFISIKDEAGQPLLTADEIKPTIKELVMAAPDNPSNAVEWAIAEMINKP 367

Query: 363 QILQTATEELDRVVGKQRLVQESDLPNLKFLTACAREALRLHPIAPFNLPHIATADAVVA 422
           +IL  A EE+DRVVGK+R VQESD+P L ++ A  REA RLHP+A FNLPH+A +D  VA
Sbjct: 368 EILHKAMEEIDRVVGKERFVQESDIPKLNYVKAIIREAFRLHPVAAFNLPHVALSDTTVA 427

Query: 423 GYFIPAGSHVLLSRLGLGRNPRIWPDPMRFDPNRHLQDPGADPNLGEPDLRFITFTRGRR 482
           GY IP GS VLLSR GLGRNP++W DP+ F P RHL +  ++  L E DLRFI+F+ G+R
Sbjct: 428 GYHIPKGSQVLLSRYGLGRNPKVWSDPLSFKPERHLNE-CSEVTLTENDLRFISFSTGKR 487

Query: 483 GCMGSALGTAITMMLLARLVQGFSWRLPPGAAEIDLSESD-QLFLKNPLFVFAEPRLLES 542
           GC   ALGTAIT M+LARL+QGF W+L      ++L ES   +FL  PL +  E RL E 
Sbjct: 488 GCAAPALGTAITTMMLARLLQGFKWKLAGSETRVELMESSHDMFLSKPLVLVGELRLSED 540

Query: 543 LYP 544
           LYP
Sbjct: 548 LYP 540

BLAST of Spg021805 vs. TAIR 10
Match: AT5G35917.1 (cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene )

HSP 1 Score: 490.3 bits (1261), Expect = 2.0e-138
Identity = 258/529 (48.77%), Postives = 344/529 (65.03%), Query Frame = 0

Query: 28  SFMAAVL-IWLFSYYGKRKQSVGT---------AALPPGPSPWPIVGCLPTI-WRRKPRY 87
           S +A +L + L S   KRK++  T          +LPPGP  WP+VG LP I  R KP +
Sbjct: 7   SMLAFILGLLLLSLTMKRKETKKTMLMTSHTRNLSLPPGPKSWPLVGNLPEILGRNKPVF 66

Query: 88  RWIHSVMEDLNTEIACIRLGKTHVIPVMSPELAVEFLKTHDSLFASRPITVTTDMFSDGF 147
           RWIHS+ME+LNT+IACIRL  THVIPV SP +A E LK  DS+FA RP+T+ T+  S G+
Sbjct: 67  RWIHSLMEELNTDIACIRLANTHVIPVTSPRIAREILKKQDSVFAIRPLTMGTEYCSRGY 126

Query: 148 LTAGVAPWGDQWRKMRRVLTSEILSPARHRWMLAKRTEEADNLLRYVFHLTTTGNDDDDV 207
           LT  V   G+QW+KMRRV+ S + S    + ML KRTEEADNL+RY+ +       + + 
Sbjct: 127 LTIAVQSQGEQWKKMRRVVASHVTSKKSFKLMLEKRTEEADNLVRYINNRCVKNRGNGN- 186

Query: 208 GGGAVVNLRSITQHYSGNVMRRMMFDRRYYGGGRAD-GGPTAEEEEHIGALFTMLQHVYA 267
            G AV++LR + + YSGNV R+MMF  R++G G  D  GP +EE EH+ +LFT+L H+YA
Sbjct: 187 -GLAVIDLRFVVRQYSGNVARKMMFGIRHFGKGSEDESGPGSEEIEHVESLFTVLTHLYA 246

Query: 268 FCVSDYMPCLKAFDLDGHERTVKKALNVIRKYEEPIIDERVQQWRDGRRTEAEDMLDIFI 327
           F +SDY+P L+  DL+GHE+ V  A+  + KY +P +DER+ QWR+G+  E +D LD+FI
Sbjct: 247 FALSDYVPWLRFLDLEGHEKVVADAMRNVSKYNDPFVDERLMQWRNGKMKEPQDFLDMFI 306

Query: 328 SLKDGDGRPLLSVREIKAQITELLLATVDNPSNAVEWAIAEMLNQPQILQTATEELDRVV 387
             KD +G+P LS  EIKAQ+TEL+LATVDNPSNA EWA+AEM+N+P I+Q A EE+DR  
Sbjct: 307 MAKDTNGKPTLSEEEIKAQVTELMLATVDNPSNAAEWAMAEMINEPSIMQKAVEEIDR-- 366

Query: 388 GKQRLVQESDLPNLKFLTACAREALRLHPIAPFNLPHIATADAVVAGYFIPAGSHVLLSR 447
                       NL+   +   E  RLHP+APFNLPH++TADAVV GYFIP GSH+L+SR
Sbjct: 367 -----------SNLR--ESVCEETFRLHPVAPFNLPHMSTADAVVDGYFIPKGSHMLISR 426

Query: 448 LGLGRNPRIWPDPMRFDPNRHLQDPGADPNLGEPDLRFITFTRGRRGCMGSALGTAITMM 507
           +G+GRNP +W  P++F+P RHL +                              +++T M
Sbjct: 427 MGIGRNPNVWDKPLKFNPERHLGN-----------------------------NSSMTYM 486

Query: 508 LLARLVQGFSWRLPPGAAEIDLSES-DQLFLKNPLFVFAEPRLLESLYP 544
           LLARL+QGF+W   PG ++ID+SES   LF+  PL   A PRL   +YP
Sbjct: 487 LLARLIQGFTWSPVPGESKIDISESKSDLFMAKPLHAVATPRLDPHVYP 489

BLAST of Spg021805 vs. TAIR 10
Match: AT1G79370.1 (cytochrome P450, family 79, subfamily C, polypeptide 1 )

HSP 1 Score: 423.7 bits (1088), Expect = 2.3e-118
Identity = 227/548 (41.42%), Postives = 340/548 (62.04%), Query Frame = 0

Query: 14  LLAICKSFMIFLTGSFMAAVLIWLFSYYGKRKQSVGTAALPPGPSPWPIVGCLPTIWRRK 73
           + ++  +F I L   F+   ++  F   G+RK+      LPP P  +PI+G L  + + +
Sbjct: 9   IFSVVVTFSITLNIVFLIKSVVARF--LGRRKK------LPPCPRGFPIIGNLVGMLKNR 68

Query: 74  PRYRWIHSVMEDLNTEIACIRLGKTHVIPVMSPELAVEFLKTHDSLFASRPITVTTDMFS 133
           P  +WI  VM D+ T+IAC R G+ HVI + S  +A E ++  D++FA RP + + +  S
Sbjct: 69  PTSKWIVRVMNDMKTDIACFRFGRVHVIVITSDVIAREVVREKDAVFADRPDSYSAEYIS 128

Query: 134 DGFLTAGVAPWGDQWRKMRRVLTSEILSPARHRWMLAKRTEEADNLLRYVFHLTTTGNDD 193
            G+       +G++  KM++V++SE++S      +L  R  E+DNLL YV +L     D+
Sbjct: 129 GGYNGVVFDEYGERQMKMKKVMSSELMSTKALNLLLKVRNLESDNLLAYVHNL--YNKDE 188

Query: 194 DDVGGGAVVNLRSITQHYSGNVMRRMMFDRRYYGGGRADGGPTAEEEEHIGALFTMLQHV 253
                GAVVN+R I   ++ NV  R++F RR++     DG     E+EH  A+F  L   
Sbjct: 189 SKTKHGAVVNVRDIVCTHTHNVKMRLLFGRRHFKETTMDGSLGLMEKEHFDAIFAALDCF 248

Query: 254 YAFCVSDYMPCLKAFDLDGHERTVKKALNVIRKYEEPIIDERVQQWR----DGRRTEA-- 313
           ++F V+DY P L+ ++L G E  +++A++VI +Y + IIDE+++ WR    D  R E   
Sbjct: 249 FSFYVADYYPFLRGWNLQGEEAELREAVDVIARYNKMIIDEKIELWRGQNKDYNRAETKN 308

Query: 314 -----EDMLDIFISLKDGDGRPLLSVREIKAQITELLLATVDNPSNAVEWAIAEMLNQPQ 373
                +D LDI  +LKD +G+PLL+ +EI     +L +  +DN  N +EW +AEMLNQ +
Sbjct: 309 DVPMIKDWLDILFTLKDENGKPLLTPQEITHLSVDLDVVGIDNAVNVIEWTLAEMLNQRE 368

Query: 374 ILQTATEELDRVVGKQRLVQESDLPNLKFLTACAREALRLHPIAPFNLPHIATADAVVAG 433
           IL+ A EE+D VVGK+RLVQESD+PNL ++ AC RE LRLHP  PF +PH+A  D  +AG
Sbjct: 369 ILEKAVEEIDMVVGKERLVQESDVPNLNYVKACCRETLRLHPTNPFLVPHMARHDTTLAG 428

Query: 434 YFIPAGSHVLLSRLGLGRNPRIWPDPMRFDPNRHLQDPGADPNLGEPDLRFITFTRGRRG 493
           YFIP GSH+L+SR G+GRNP+ W +P+ + P RH+   G +  L EPDLR ++F  GRRG
Sbjct: 429 YFIPKGSHILVSRPGVGRNPKTWDEPLIYRPERHI--TGNEVVLTEPDLRLVSFGTGRRG 488

Query: 494 CMGSALGTAITMMLLARLVQGFSWRLPPGAAE-IDLSES-DQLFLKNPLFVFAEPRLLES 549
           C+G+ LGT++ + LL RL+QGF W +PPG  + ++L ES + LF+ NPL    +PRL  +
Sbjct: 489 CVGAKLGTSMIVTLLGRLLQGFDWTIPPGTTDRVELVESKENLFMANPLMACVKPRLDPN 544

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK27737.18.3e-25681.60tyrosine N-monooxygenase [Cucumis melo var. makuwa][more]
KAA0056052.11.6e-25481.24tyrosine N-monooxygenase [Cucumis melo var. makuwa][more]
XP_004139121.13.5e-25480.87tyrosine N-monooxygenase [Cucumis sativus] >KGN66827.2 hypothetical protein Csa_... [more]
XP_008450479.11.3e-25383.95PREDICTED: tyrosine N-monooxygenase [Cucumis melo][more]
KAG2675236.11.9e-20769.00hypothetical protein I3760_13G174900 [Carya illinoinensis][more]
Match NameE-valueIdentityDescription
A0A068Q6058.8e-18458.17Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV... [more]
Q6J5401.2e-16753.11Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1[more]
Q6J5413.7e-16653.45Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1[more]
Q431356.3e-16654.12Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3[more]
Q9M7B83.5e-16454.86Valine N-monooxygenase 1 OS=Manihot esculenta OX=3983 GN=CYP79D1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3DVB84.0e-25681.60Tyrosine N-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A5A7UJK27.6e-25581.24Tyrosine N-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A1S3BPC76.4e-25483.95tyrosine N-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103492071 PE=3 SV=1[more]
A0A7N2KLH41.7e-20164.05Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1[more]
A0A6J1D6G99.7e-19463.35isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498... [more]
Match NameE-valueIdentityDescription
AT5G05260.12.3e-16356.16cytochrome p450 79a2 [more]
AT4G39950.15.5e-15752.54cytochrome P450, family 79, subfamily B, polypeptide 2 [more]
AT2G22330.13.3e-15448.80cytochrome P450, family 79, subfamily B, polypeptide 3 [more]
AT5G35917.12.0e-13848.77cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene [more]
AT1G79370.12.3e-11841.42cytochrome P450, family 79, subfamily C, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 350..376
score: 32.06
coord: 473..483
score: 44.74
coord: 108..129
score: 26.22
coord: 330..347
score: 18.78
coord: 211..229
score: 22.42
coord: 393..411
score: 35.99
coord: 483..506
score: 28.06
coord: 434..458
score: 27.49
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 48..538
e-value: 1.5E-100
score: 339.1
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 52..536
IPR001128Cytochrome P450PFAMPF00067p450coord: 54..527
e-value: 2.9E-80
score: 270.3
NoneNo IPR availablePANTHERPTHR24298:SF675PHENYLALANINE N-MONOOXYGENASEcoord: 31..541
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 31..541

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg021805.1Spg021805.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen