Spg020685 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg020685
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAP2/ERF and B3 domain-containing transcription factor RAV1-like
Locationscaffold10: 28501189 .. 28502169 (+)
RNA-Seq ExpressionSpg020685
SyntenySpg020685
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGCAAGCTCTGTAGACGAAAGCACCGCCAGTGACTCCACCTCCATTTCTCCCGTCACCCCATTCCTCTTCAAGTCATCGCCGTCGCCGGCCGCCGTCGGGATTCTGCACGAGGCCGAATCCCGCAAGCTTCCATCTTCCCGATTCAAAGGAGTGGTCCCTCAGCCCAACGGCCGATGGGGGGCTCAGATTTACGAGAAGCACCAACGCGTCTGGCTCGGCACATTCAACGAGGAGCACGACGCCGCCAAAGCCTACGACATCGCCGCCCTCCGCTTCCGCGGCCGCGACGCTGTCACCAACTTCAAGCCCGACGCCTCCGCCACCGCCACCGCCGACGCCGCCGTCGAGGCCGACTTCCTCAAATCCCACTCCAAGCTGGAGATCGTCGACATGCTCCGCAAGCACACCTACAATGACGAGCTCCAGCAGAGCAAGCGCCACCGCGGGGCCGCTCCGGCTCCGACGTCTCTCGAGCAGGACGGCTCCGATCGTGGGTCGGATTGTTCGCGGGAGGTGCTGTTCGAGAAAAGCGTGACGCCGAGCGACGTGGGGAAGTTGAACCGGCTGGTGATTCCGAAGCAGCACGCGGAGAAGAACTTCCCGTTGGGGGACGGCGTGGTTTCTTCGGCGAAAGGAATGCTTTTGAATTTCGAGGATATGGGAGGGAAGGTGTGGCGGTTCCGGTACTCGTACTGGAACAGCAGCCAGAGCTATGTGTTGACCAAGGGATGGAGCCGGTTCGTGAAAGAGAAGAGTCTCCGAGCCGGGGATGTGGTCCGGTTCCTCCGGTCGACCGGACCGGATAAGCAGCTTTATATTGATGGGAGACCGGGAGGGGTGAGGGCGGTCCAAGTGGTGAAGCTTTTTGGAGTGAATATTTTGGAAGTGCCGGTGGGTTGTTGTGGAAAGAGAGAGACAGAAGGGTTGTTGGATTTACAAGGAAGTAAGAAGCAAAGAATAATCAAAGCTTTGTAA

mRNA sequence

ATGGACGCAAGCTCTGTAGACGAAAGCACCGCCAGTGACTCCACCTCCATTTCTCCCGTCACCCCATTCCTCTTCAAGTCATCGCCGTCGCCGGCCGCCGTCGGGATTCTGCACGAGGCCGAATCCCGCAAGCTTCCATCTTCCCGATTCAAAGGAGTGGTCCCTCAGCCCAACGGCCGATGGGGGGCTCAGATTTACGAGAAGCACCAACGCGTCTGGCTCGGCACATTCAACGAGGAGCACGACGCCGCCAAAGCCTACGACATCGCCGCCCTCCGCTTCCGCGGCCGCGACGCTGTCACCAACTTCAAGCCCGACGCCTCCGCCACCGCCACCGCCGACGCCGCCGTCGAGGCCGACTTCCTCAAATCCCACTCCAAGCTGGAGATCGTCGACATGCTCCGCAAGCACACCTACAATGACGAGCTCCAGCAGAGCAAGCGCCACCGCGGGGCCGCTCCGGCTCCGACGTCTCTCGAGCAGGACGGCTCCGATCGTGGGTCGGATTGTTCGCGGGAGGTGCTGTTCGAGAAAAGCGTGACGCCGAGCGACGTGGGGAAGTTGAACCGGCTGGTGATTCCGAAGCAGCACGCGGAGAAGAACTTCCCGTTGGGGGACGGCGTGGTTTCTTCGGCGAAAGGAATGCTTTTGAATTTCGAGGATATGGGAGGGAAGGTGTGGCGGTTCCGGTACTCGTACTGGAACAGCAGCCAGAGCTATGTGTTGACCAAGGGATGGAGCCGGTTCGTGAAAGAGAAGAGTCTCCGAGCCGGGGATGTGGTCCGGTTCCTCCGGTCGACCGGACCGGATAAGCAGCTTTATATTGATGGGAGACCGGGAGGGGTGAGGGCGGTCCAAGTGGTGAAGCTTTTTGGAGTGAATATTTTGGAAGTGCCGGTGGGTTGTTGTGGAAAGAGAGAGACAGAAGGGTTGTTGGATTTACAAGGAAGTAAGAAGCAAAGAATAATCAAAGCTTTGTAA

Coding sequence (CDS)

ATGGACGCAAGCTCTGTAGACGAAAGCACCGCCAGTGACTCCACCTCCATTTCTCCCGTCACCCCATTCCTCTTCAAGTCATCGCCGTCGCCGGCCGCCGTCGGGATTCTGCACGAGGCCGAATCCCGCAAGCTTCCATCTTCCCGATTCAAAGGAGTGGTCCCTCAGCCCAACGGCCGATGGGGGGCTCAGATTTACGAGAAGCACCAACGCGTCTGGCTCGGCACATTCAACGAGGAGCACGACGCCGCCAAAGCCTACGACATCGCCGCCCTCCGCTTCCGCGGCCGCGACGCTGTCACCAACTTCAAGCCCGACGCCTCCGCCACCGCCACCGCCGACGCCGCCGTCGAGGCCGACTTCCTCAAATCCCACTCCAAGCTGGAGATCGTCGACATGCTCCGCAAGCACACCTACAATGACGAGCTCCAGCAGAGCAAGCGCCACCGCGGGGCCGCTCCGGCTCCGACGTCTCTCGAGCAGGACGGCTCCGATCGTGGGTCGGATTGTTCGCGGGAGGTGCTGTTCGAGAAAAGCGTGACGCCGAGCGACGTGGGGAAGTTGAACCGGCTGGTGATTCCGAAGCAGCACGCGGAGAAGAACTTCCCGTTGGGGGACGGCGTGGTTTCTTCGGCGAAAGGAATGCTTTTGAATTTCGAGGATATGGGAGGGAAGGTGTGGCGGTTCCGGTACTCGTACTGGAACAGCAGCCAGAGCTATGTGTTGACCAAGGGATGGAGCCGGTTCGTGAAAGAGAAGAGTCTCCGAGCCGGGGATGTGGTCCGGTTCCTCCGGTCGACCGGACCGGATAAGCAGCTTTATATTGATGGGAGACCGGGAGGGGTGAGGGCGGTCCAAGTGGTGAAGCTTTTTGGAGTGAATATTTTGGAAGTGCCGGTGGGTTGTTGTGGAAAGAGAGAGACAGAAGGGTTGTTGGATTTACAAGGAAGTAAGAAGCAAAGAATAATCAAAGCTTTGTAA

Protein sequence

MDASSVDESTASDSTSISPVTPFLFKSSPSPAAVGILHEAESRKLPSSRFKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNFKPDASATATADAAVEADFLKSHSKLEIVDMLRKHTYNDELQQSKRHRGAAPAPTSLEQDGSDRGSDCSREVLFEKSVTPSDVGKLNRLVIPKQHAEKNFPLGDGVVSSAKGMLLNFEDMGGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYIDGRPGGVRAVQVVKLFGVNILEVPVGCCGKRETEGLLDLQGSKKQRIIKAL
Homology
BLAST of Spg020685 vs. NCBI nr
Match: XP_023541268.1 (AP2/ERF and B3 domain-containing transcription factor RAV1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.4 bits (1300), Expect = 3.7e-139
Identity = 256/325 (78.77%), Postives = 283/325 (87.08%), Query Frame = 0

Query: 4   SSVDESTASDSTSISPVTPFLFKSSPSPAAVGILHEAESRKLPSSRFKGVVPQPNGRWGA 63
           SS+D+S ASDSTS+SPVTP LF SS S    G+ ++AESRKLPSSRFKGVVPQPNGRWGA
Sbjct: 5   SSLDDSAASDSTSVSPVTPLLFHSSSSSCPPGVPYDAESRKLPSSRFKGVVPQPNGRWGA 64

Query: 64  QIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNFKPDASATATADAAVEADFLK 123
           QIYEKHQRVWLGTFN EH AAKAYDIAALRFRGRDAVTNFKP   A ++ DAA EADFL 
Sbjct: 65  QIYEKHQRVWLGTFNHEHQAAKAYDIAALRFRGRDAVTNFKP--GAFSSVDAAFEADFLN 124

Query: 124 SHSKLEIVDMLRKHTYNDELQQSKRHRGAAPAPTSLEQDGS--DRGSDCSREVLFEKSVT 183
           SHSKLEIVDMLRKHTYNDE+QQSKR+ G     T+     S  +  S+  REVLFEK+VT
Sbjct: 125 SHSKLEIVDMLRKHTYNDEIQQSKRYGGGTTTTTTAAAAHSLQNAASESDREVLFEKTVT 184

Query: 184 PSDVGKLNRLVIPKQHAEKNFPLGDGVVSSAKGMLLNFEDMGGKVWRFRYSYWNSSQSYV 243
           PSDVGKLNRLVIPKQHAEKNFPLG+G + S KG+LLNFEDMGGK+WRFRYSYWNSSQSYV
Sbjct: 185 PSDVGKLNRLVIPKQHAEKNFPLGEG-MGSTKGILLNFEDMGGKLWRFRYSYWNSSQSYV 244

Query: 244 LTKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYIDGRPGGVRAVQVVKLFGVNILEVPVG 303
           LTKGWSRFVKEK+LRAGDVVRFLRSTGPDK+LYIDG+PGGV+AVQVVKLFGVNILEVPVG
Sbjct: 245 LTKGWSRFVKEKTLRAGDVVRFLRSTGPDKKLYIDGKPGGVKAVQVVKLFGVNILEVPVG 304

Query: 304 CCGKRETEGLLDLQGSKKQRIIKAL 327
            CGKR+ EGL +L+ +KKQRI+KAL
Sbjct: 305 WCGKRDREGLSELERTKKQRIVKAL 326

BLAST of Spg020685 vs. NCBI nr
Match: KAG6601748.1 (AP2/ERF and B3 domain-containing transcription factor ARF14, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 505.0 bits (1299), Expect = 4.9e-139
Identity = 257/325 (79.08%), Postives = 283/325 (87.08%), Query Frame = 0

Query: 4   SSVDESTASDSTSISPVTPFLFKSSPSPAAVGILHEAESRKLPSSRFKGVVPQPNGRWGA 63
           SS+D+S ASDSTS+SPVTP LF SS S ++ G+ H+AESRKLPSSRFKGVVPQPNGRWGA
Sbjct: 5   SSLDDSAASDSTSVSPVTPLLFHSSSSSSSPGVPHDAESRKLPSSRFKGVVPQPNGRWGA 64

Query: 64  QIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNFKPDASATATADAAVEADFLK 123
           QIYEKHQRVWLGTFN EH AAKAYDIAALRFRGRDAVTNFKP   A ++ DAA EADFL 
Sbjct: 65  QIYEKHQRVWLGTFNHEHQAAKAYDIAALRFRGRDAVTNFKP--GAFSSVDAAFEADFLN 124

Query: 124 SHSKLEIVDMLRKHTYNDELQQSKRHRGAAPAPTSLEQDGS--DRGSDCSREVLFEKSVT 183
           SHSKLEIVDMLRKHTYNDELQQSKR+ G     T+     S  +  S+  REVLFEK+VT
Sbjct: 125 SHSKLEIVDMLRKHTYNDELQQSKRYGGGTTTTTTAAAAHSLQNAASESDREVLFEKTVT 184

Query: 184 PSDVGKLNRLVIPKQHAEKNFPLGDGVVSSAKGMLLNFEDMGGKVWRFRYSYWNSSQSYV 243
           PSDVGKLNRLVIPKQHAEKNFPLG+G + SAKG+LLNFEDMGGK+WRFRYSYWNSSQSYV
Sbjct: 185 PSDVGKLNRLVIPKQHAEKNFPLGEG-MGSAKGLLLNFEDMGGKLWRFRYSYWNSSQSYV 244

Query: 244 LTKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYIDGRPGGVRAVQVVKLFGVNILEVPVG 303
           LTKGWSRFVKEK+LRAGDVVRFLRSTGPDK+LYIDG+ GGV+ VQVVKLFGVNILEVPVG
Sbjct: 245 LTKGWSRFVKEKTLRAGDVVRFLRSTGPDKKLYIDGKAGGVKPVQVVKLFGVNILEVPVG 304

Query: 304 CCGKRETEGLLDLQGSKKQRIIKAL 327
            CGKR+ EGL +L+ +KKQRI KAL
Sbjct: 305 WCGKRDREGLSELERAKKQRITKAL 326

BLAST of Spg020685 vs. NCBI nr
Match: KAG7032471.1 (AP2/ERF and B3 domain-containing transcription repressor TEM1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 504.6 bits (1298), Expect = 6.4e-139
Identity = 256/324 (79.01%), Postives = 282/324 (87.04%), Query Frame = 0

Query: 4   SSVDESTASDSTSISPVTPFLFKSSPSPAAVGILHEAESRKLPSSRFKGVVPQPNGRWGA 63
           SS+D+S ASDSTS+SPVTP LF SS S ++ G+ H+AESRKLPSSRFKGVVPQPNGRWGA
Sbjct: 5   SSLDDSAASDSTSVSPVTPLLFHSSSSSSSPGVPHDAESRKLPSSRFKGVVPQPNGRWGA 64

Query: 64  QIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNFKPDASATATADAAVEADFLK 123
           QIYEKHQRVWLGTFN EH AAKAYDIAALRFRGRDAVTNFKP   A ++ DAA EADFL 
Sbjct: 65  QIYEKHQRVWLGTFNHEHQAAKAYDIAALRFRGRDAVTNFKP--GAFSSVDAAFEADFLN 124

Query: 124 SHSKLEIVDMLRKHTYNDELQQSKRHRGAAPAPTSLEQDG-SDRGSDCSREVLFEKSVTP 183
           SHSKLEIVDMLRKHTYNDELQQSKR+ G     T+       +  S+  REVLFEK+VTP
Sbjct: 125 SHSKLEIVDMLRKHTYNDELQQSKRYGGGTTTTTTAAAHSLQNAASESDREVLFEKTVTP 184

Query: 184 SDVGKLNRLVIPKQHAEKNFPLGDGVVSSAKGMLLNFEDMGGKVWRFRYSYWNSSQSYVL 243
           SDVGKLNRLVIPKQHAEKNFPLG+G + SAKG+LLNFEDMGGK+WRFRYSYWNSSQSYVL
Sbjct: 185 SDVGKLNRLVIPKQHAEKNFPLGEG-MGSAKGVLLNFEDMGGKLWRFRYSYWNSSQSYVL 244

Query: 244 TKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYIDGRPGGVRAVQVVKLFGVNILEVPVGC 303
           TKGWSRFVKEK+LRAGDVVRFLRSTGPDK+LYIDG+ GGV+ VQVVKLFGVNILEVPVG 
Sbjct: 245 TKGWSRFVKEKTLRAGDVVRFLRSTGPDKKLYIDGKAGGVKPVQVVKLFGVNILEVPVGW 304

Query: 304 CGKRETEGLLDLQGSKKQRIIKAL 327
           CGKR+ EGL +L+ +KKQRI KAL
Sbjct: 305 CGKRDREGLSELERAKKQRITKAL 325

BLAST of Spg020685 vs. NCBI nr
Match: XP_022930067.1 (AP2/ERF and B3 domain-containing transcription factor RAV1-like [Cucurbita moschata])

HSP 1 Score: 504.2 bits (1297), Expect = 8.4e-139
Identity = 259/327 (79.20%), Postives = 283/327 (86.54%), Query Frame = 0

Query: 4   SSVDESTASDSTSISPVTPFLFKSSPSPAAVGILHEAESRKLPSSRFKGVVPQPNGRWGA 63
           SS+D+S ASDSTS+SPVTP LF SS SP   G+ H+AESRKLPSSRFKGVVPQPNGRWGA
Sbjct: 5   SSLDDSAASDSTSVSPVTPLLFHSSSSP---GVPHDAESRKLPSSRFKGVVPQPNGRWGA 64

Query: 64  QIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNFKPDASATATADAAVEADFLK 123
           QIYEKHQRVWLGTFN EH AAKAYDIAALRFRGRDAVTNFKP   A ++ DAA EADFL 
Sbjct: 65  QIYEKHQRVWLGTFNHEHQAAKAYDIAALRFRGRDAVTNFKP--GAFSSVDAAFEADFLN 124

Query: 124 SHSKLEIVDMLRKHTYNDELQQSKRHRGAAPAPTSLEQDGS----DRGSDCSREVLFEKS 183
           SHSKLEIVDMLRKHTYNDELQQSKR+ G     T+     +    +  S+  REVLFEK+
Sbjct: 125 SHSKLEIVDMLRKHTYNDELQQSKRYGGGTTTTTTTAAAAAHSLQNAASESDREVLFEKT 184

Query: 184 VTPSDVGKLNRLVIPKQHAEKNFPLGDGVVSSAKGMLLNFEDMGGKVWRFRYSYWNSSQS 243
           VTPSDVGKLNRLVIPKQHAEKNFPLG+G + SAKG+LLNFEDMGGK+WRFRYSYWNSSQS
Sbjct: 185 VTPSDVGKLNRLVIPKQHAEKNFPLGEG-MGSAKGVLLNFEDMGGKLWRFRYSYWNSSQS 244

Query: 244 YVLTKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYIDGRPGGVRAVQVVKLFGVNILEVP 303
           YVLTKGWSRFVKEK+LRAGDVVRFLRSTGPDK+LYIDGR GGV+ VQVVKLFGVNILEVP
Sbjct: 245 YVLTKGWSRFVKEKTLRAGDVVRFLRSTGPDKKLYIDGRAGGVKPVQVVKLFGVNILEVP 304

Query: 304 VGCCGKRETEGLLDLQGSKKQRIIKAL 327
           VG CGKR+ EGL +L+ +KKQRIIKAL
Sbjct: 305 VGWCGKRDREGLSELERTKKQRIIKAL 325

BLAST of Spg020685 vs. NCBI nr
Match: XP_022973620.1 (AP2/ERF and B3 domain-containing transcription factor RAV1-like [Cucurbita maxima])

HSP 1 Score: 500.7 bits (1288), Expect = 9.2e-138
Identity = 254/326 (77.91%), Postives = 284/326 (87.12%), Query Frame = 0

Query: 4   SSVDESTASDSTSISPVTPFLFKSSPSPAAVGILHEAESRKLPSSRFKGVVPQPNGRWGA 63
           SS+D+STASDSTS+SPVTP LF SS S +  G+ ++AESRKLPSSRFKGVVPQPNGRWGA
Sbjct: 5   SSLDDSTASDSTSVSPVTPLLFHSSSSSSPPGVPYDAESRKLPSSRFKGVVPQPNGRWGA 64

Query: 64  QIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNFKPDASATATADAAVEADFLK 123
           QIYEKHQRVWLGTFN+EH AAKAYDIAALR+RGRDAVTNFKP   A ++ DAA EADFL 
Sbjct: 65  QIYEKHQRVWLGTFNQEHQAAKAYDIAALRYRGRDAVTNFKP--GAFSSVDAAFEADFLN 124

Query: 124 SHSKLEIVDMLRKHTYNDELQQSKRHRG---AAPAPTSLEQDGSDRGSDCSREVLFEKSV 183
           SHSKLEIVDMLRKHTYNDELQQSKR+ G        T+      +  S+  REVLFEK+V
Sbjct: 125 SHSKLEIVDMLRKHTYNDELQQSKRYGGGTTTTATATAAAHSLHNAASESDREVLFEKTV 184

Query: 184 TPSDVGKLNRLVIPKQHAEKNFPLGDGVVSSAKGMLLNFEDMGGKVWRFRYSYWNSSQSY 243
           TPSDVGKLNRLVIPKQHAEKNFPLG+G + SAKG+LLNFEDMGGK+WRFRYSYWNSSQSY
Sbjct: 185 TPSDVGKLNRLVIPKQHAEKNFPLGEG-MGSAKGVLLNFEDMGGKLWRFRYSYWNSSQSY 244

Query: 244 VLTKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYIDGRPGGVRAVQVVKLFGVNILEVPV 303
           VLTKGWSRFVKEK+LRAGDVVRFLRSTGPDK+LYIDG+ GGV+ VQVV+LFGVNILEVPV
Sbjct: 245 VLTKGWSRFVKEKTLRAGDVVRFLRSTGPDKKLYIDGKAGGVKPVQVVRLFGVNILEVPV 304

Query: 304 GCCGKRETEGLLDLQGSKKQRIIKAL 327
           G CGKR+ EGL +L+ +KKQR+IKAL
Sbjct: 305 GWCGKRDREGLSELERTKKQRMIKAL 327

BLAST of Spg020685 vs. ExPASy Swiss-Prot
Match: P82280 (AP2/ERF and B3 domain-containing transcription repressor RAV2 OS=Arabidopsis thaliana OX=3702 GN=RAV2 PE=1 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 2.6e-95
Identity = 204/361 (56.51%), Postives = 244/361 (67.59%), Query Frame = 0

Query: 1   MDASSVDESTASDSTSISPVTPFLFKSSPSPAAVGILH-----------------EAESR 60
           MD+S +DE ++S S S S  T    K SP PAA   L+                 E ESR
Sbjct: 1   MDSSCIDEISSSTSESFSATT--AKKLSPPPAAALRLYRMGSGGSSVVLDPENGLETESR 60

Query: 61  KLPSSRFKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNF 120
           KLPSS++KGVVPQPNGRWGAQIYEKHQRVWLGTFNE+ +AA++YDIAA RFRGRDAV NF
Sbjct: 61  KLPSSKYKGVVPQPNGRWGAQIYEKHQRVWLGTFNEQEEAARSYDIAACRFRGRDAVVNF 120

Query: 121 KPDASATATADAAVEADFLKSHSKLEIVDMLRKHTYNDELQQSKRHRGAAPAPTSLEQDG 180
           K   +     D A    FL++HSK EIVDMLRKHTY DEL+Q+ + +       + +++G
Sbjct: 121 K---NVLEDGDLA----FLEAHSKAEIVDMLRKHTYADELEQNNKRQLFLSVDANGKRNG 180

Query: 181 SDRGSD----CSREVLFEKSVTPSDVGKLNRLVIPKQHAEKNFPLGDGVVSSAKGMLLNF 240
           S    +     +REVLFEK+VTPSDVGKLNRLVIPKQHAEK+FPL     +  KG+L+NF
Sbjct: 181 SSTTQNDKVLKTREVLFEKAVTPSDVGKLNRLVIPKQHAEKHFPLPSPSPAVTKGVLINF 240

Query: 241 EDMGGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYIDGR- 300
           ED+ GKVWRFRYSYWNSSQSYVLTKGWSRFVKEK+LRAGDVV F RSTG ++QLYID + 
Sbjct: 241 EDVNGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKNLRAGDVVTFERSTGLERQLYIDWKV 300

Query: 301 ---PGGVRAVQVVKLFGVNILEVP-------VGCCG---KRETEGLLDLQGSKKQRIIKA 327
              P       VV+LFGV+I  V        V  CG    R+ + +  L+ SKKQ II A
Sbjct: 301 RSGPRENPVQVVVRLFGVDIFNVTTVKPNDVVAVCGGKRSRDVDDMFALRCSKKQAIINA 352

BLAST of Spg020685 vs. ExPASy Swiss-Prot
Match: Q9ZWM9 (AP2/ERF and B3 domain-containing transcription factor RAV1 OS=Arabidopsis thaliana OX=3702 GN=RAV1 PE=1 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 5.0e-94
Identity = 211/353 (59.77%), Postives = 240/353 (67.99%), Query Frame = 0

Query: 1   MDASSVDESTAS-----DSTSISPVTPF----LFKSSPSPAAV-----GILHEAESRKLP 60
           M++SSVDEST S     ++ +I+P        L++     + V     G+  EAESRKLP
Sbjct: 1   MESSSVDESTTSTGSICETPAITPAKKSSVGNLYRMGSGSSVVLDSENGV--EAESRKLP 60

Query: 61  SSRFKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNFKPD 120
           SS++KGVVPQPNGRWGAQIYEKHQRVWLGTFNEE +AA+AYD+A  RFR RDAVTNFK  
Sbjct: 61  SSKYKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEDEAARAYDVAVHRFRRRDAVTNFK-- 120

Query: 121 ASATATADAAVEADFLKSHSKLEIVDMLRKHTYNDELQQSKRHRGAAPAPT------SLE 180
                  D   E DFL SHSK EIVDMLRKHTYN+EL+QSKR R      T       L 
Sbjct: 121 -DVKMDED---EVDFLNSHSKSEIVDMLRKHTYNEELEQSKRRRNGNGNMTRTLLTSGLS 180

Query: 181 QDGSDRGSDCSREVLFEKSVTPSDVGKLNRLVIPKQHAEKNFPLGDGVVSSAKGMLLNFE 240
            DG       S E LFEK+VTPSDVGKLNRLVIPK HAEK+FPL    V S KG+LLNFE
Sbjct: 181 NDGVSTTGFRSAEALFEKAVTPSDVGKLNRLVIPKHHAEKHFPLPSSNV-SVKGVLLNFE 240

Query: 241 DMGGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYI--DGR 300
           D+ GKVWRFRYSYWNSSQSYVLTKGWSRFVKEK+LRAGDVV F RS G D+QLYI    R
Sbjct: 241 DVNGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKNLRAGDVVSFSRSNGQDQQLYIGWKSR 300

Query: 301 PGG-VRAVQVVKLFGVNILEVP-----VGCCGKRETEGLLDLQGSKKQRIIKA 326
            G  + A +V++LFGVNI         VG     +TE +L L  SKKQRI  A
Sbjct: 301 SGSDLDAGRVLRLFGVNISPESSRNDVVGNKRVNDTE-MLSLVCSKKQRIFHA 343

BLAST of Spg020685 vs. ExPASy Swiss-Prot
Match: Q9C6M5 (AP2/ERF and B3 domain-containing transcription repressor TEM1 OS=Arabidopsis thaliana OX=3702 GN=TEM1 PE=1 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 7.2e-93
Identity = 210/367 (57.22%), Postives = 238/367 (64.85%), Query Frame = 0

Query: 1   MDASSVDE-STASDSTSIS----PVTPFLFKSSPSPAAVGILH----------------- 60
           M+ S VD+ ST S+S SIS    P T    K S  PA    L+                 
Sbjct: 1   MEYSCVDDSSTTSESLSISTTPKPTTTTEKKLSSPPATSMRLYRMGSGGSSVVLDSENGV 60

Query: 61  EAESRKLPSSRFKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRD 120
           E ESRKLPSS++KGVVPQPNGRWGAQIYEKHQRVWLGTFNEE +AA +YDIA  RFRGRD
Sbjct: 61  ETESRKLPSSKYKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEEEAASSYDIAVRRFRGRD 120

Query: 121 AVTNFKPDASATATADAAVEADFLKSHSKLEIVDMLRKHTYNDELQQSKRHRGAAPAPTS 180
           AVTNFK         DA  E+ FL +HSK EIVDMLRKHTY DE +QS+R         S
Sbjct: 121 AVTNFKSQVDGN---DA--ESAFLDAHSKAEIVDMLRKHTYADEFEQSRRKFVNGDGKRS 180

Query: 181 LEQDGSDRGSDC---SREVLFEKSVTPSDVGKLNRLVIPKQHAEKNFPLGDGVV------ 240
              + +  G+D    +REVLFEK+VTPSDVGKLNRLVIPKQHAEK+FPL           
Sbjct: 181 -GLETATYGNDAVLRAREVLFEKTVTPSDVGKLNRLVIPKQHAEKHFPLPAMTTAMGMNP 240

Query: 241 SSAKGMLLNFEDMGGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKSLRAGDVVRFLRSTGP 300
           S  KG+L+N ED  GKVWRFRYSYWNSSQSYVLTKGWSRFVKEK+LRAGDVV F RSTGP
Sbjct: 241 SPTKGVLINLEDRTGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKNLRAGDVVCFERSTGP 300

Query: 301 DKQLYIDGRPGGVRAVQVVKLFGVNIL--------EVPVGCCGKRET--EGLLDLQGSKK 327
           D+QLYI  +        VV+LFGVNI         +V V C GK+ +  + L  L  SKK
Sbjct: 301 DRQLYIHWKVRSSPVQTVVRLFGVNIFNVSNEKPNDVAVECVGKKRSREDDLFSLGCSKK 360

BLAST of Spg020685 vs. ExPASy Swiss-Prot
Match: Q9LS06 (AP2/ERF and B3 domain-containing transcription factor ARF14 OS=Arabidopsis thaliana OX=3702 GN=ARF14 PE=2 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 8.5e-86
Identity = 196/342 (57.31%), Postives = 228/342 (66.67%), Query Frame = 0

Query: 1   MDA-SSVDE-STASDS----TSISPVTPFLFKSS------PSPAAVGILHEAESRKLPSS 60
           MDA SSVDE ST +DS     S SP +      S       S   V +  EAESRKLPSS
Sbjct: 1   MDAMSSVDESSTTTDSIPARKSSSPASLLYRMGSGTSVVLDSENGVEVEVEAESRKLPSS 60

Query: 61  RFKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNFKPDAS 120
           RFKGVVPQPNGRWGAQIYEKHQRVWLGTFNEE +AA+AYD+AA RFRGRDAVTNFK    
Sbjct: 61  RFKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEDEAARAYDVAAHRFRGRDAVTNFKDTTF 120

Query: 121 ATATADAAVEADFLKSHSKLEIVDMLRKHTYNDELQQSKRHRGAAPAPTSLEQDGS--DR 180
                    E +FL +HSK EIVDMLRKHTY +EL Q KR+R      T+     S    
Sbjct: 121 EE-------EVEFLNAHSKSEIVDMLRKHTYKEELDQRKRNRDGNGKETTAFALASMVVM 180

Query: 181 GSDCSREVLFEKSVTPSDVGKLNRLVIPKQHAEKNF--PLGDGVVSSAKGMLLNFEDMGG 240
               + E+LFEK+VTPSDVGKLNRLVIPK  AEK+F  PLG+  V S KGMLLNFED+ G
Sbjct: 181 TGFKTAELLFEKTVTPSDVGKLNRLVIPKHQAEKHFPLPLGNNNV-SVKGMLLNFEDVNG 240

Query: 241 KVWRFRYSYWNSSQSYVLTKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYIDGRPGG--- 300
           KVWRFRYSYWNSSQSYVLTKGWSRFVKEK L AGD++ F RS   D++ +I  +      
Sbjct: 241 KVWRFRYSYWNSSQSYVLTKGWSRFVKEKRLCAGDLISFKRSNDQDQKFFIGWKSKSGLD 300

Query: 301 VRAVQVVKLFGVNILEVPVGCCGKRETEGLLDLQGSKKQRII 324
           +   +V++LFGV+I  +      K  TE L+     KKQR++
Sbjct: 301 LETGRVMRLFGVDI-SLNAVVVVKETTEVLMSSLRCKKQRVL 333

BLAST of Spg020685 vs. ExPASy Swiss-Prot
Match: Q8RZX9 (AP2/ERF and B3 domain-containing protein Os01g0693400 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0693400 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 8.0e-84
Identity = 185/320 (57.81%), Postives = 210/320 (65.62%), Query Frame = 0

Query: 44  KLPSSRFKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNF 103
           KLPSS+FKGVVPQPNGRWGAQIYE+HQRVWLGTF  E DAA+AYD+AA RFRGRDAVTNF
Sbjct: 76  KLPSSKFKGVVPQPNGRWGAQIYERHQRVWLGTFAGEDDAARAYDVAAQRFRGRDAVTNF 135

Query: 104 KPDASATATADAAVEADFLKSHSKLEIVDMLRKHTYNDELQQSKRHRGAAPAPTS----- 163
           +P   A A  DAA E  FL + SK E+VDMLRKHTY DEL QSKR   AA  P++     
Sbjct: 136 RP--LAEADPDAAAELRFLATRSKAEVVDMLRKHTYFDELAQSKR-TFAASTPSAATTTA 195

Query: 164 ------LEQDGSDRGSDCSREVLFEKSVTPSDVGKLNRLVIPKQHAEKNFPLG-DGVVSS 223
                 L    S      +R+ LF+K+VTPSDVGKLNRLVIPKQHAEK+FPL        
Sbjct: 196 SLSNGHLSSPRSPFAPAAARDHLFDKTVTPSDVGKLNRLVIPKQHAEKHFPLQLPSAGGE 255

Query: 224 AKGMLLNFEDMGGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKSLRAGDVVRFLR---STG 283
           +KG+LLNFED  GKVWRFRYSYWNSSQSYVLTKGWSRFVKEK L AGDVV F R   S G
Sbjct: 256 SKGVLLNFEDAAGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKGLHAGDVVGFYRSAASAG 315

Query: 284 PDKQLYIDGR----PGGVRA----------VQVVKLFGVNILEVP------VGCCGKRET 327
            D +L+ID +     G   A          V+ V+LFGV++L  P       GC   R+ 
Sbjct: 316 DDGKLFIDCKLVRSTGAALASPADQPAPSPVKAVRLFGVDLLTAPAPVEQMAGCKRARDL 375

BLAST of Spg020685 vs. ExPASy TrEMBL
Match: A0A6J1EPB7 (AP2/ERF and B3 domain-containing transcription factor RAV1-like OS=Cucurbita moschata OX=3662 GN=LOC111436555 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 4.0e-139
Identity = 259/327 (79.20%), Postives = 283/327 (86.54%), Query Frame = 0

Query: 4   SSVDESTASDSTSISPVTPFLFKSSPSPAAVGILHEAESRKLPSSRFKGVVPQPNGRWGA 63
           SS+D+S ASDSTS+SPVTP LF SS SP   G+ H+AESRKLPSSRFKGVVPQPNGRWGA
Sbjct: 5   SSLDDSAASDSTSVSPVTPLLFHSSSSP---GVPHDAESRKLPSSRFKGVVPQPNGRWGA 64

Query: 64  QIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNFKPDASATATADAAVEADFLK 123
           QIYEKHQRVWLGTFN EH AAKAYDIAALRFRGRDAVTNFKP   A ++ DAA EADFL 
Sbjct: 65  QIYEKHQRVWLGTFNHEHQAAKAYDIAALRFRGRDAVTNFKP--GAFSSVDAAFEADFLN 124

Query: 124 SHSKLEIVDMLRKHTYNDELQQSKRHRGAAPAPTSLEQDGS----DRGSDCSREVLFEKS 183
           SHSKLEIVDMLRKHTYNDELQQSKR+ G     T+     +    +  S+  REVLFEK+
Sbjct: 125 SHSKLEIVDMLRKHTYNDELQQSKRYGGGTTTTTTTAAAAAHSLQNAASESDREVLFEKT 184

Query: 184 VTPSDVGKLNRLVIPKQHAEKNFPLGDGVVSSAKGMLLNFEDMGGKVWRFRYSYWNSSQS 243
           VTPSDVGKLNRLVIPKQHAEKNFPLG+G + SAKG+LLNFEDMGGK+WRFRYSYWNSSQS
Sbjct: 185 VTPSDVGKLNRLVIPKQHAEKNFPLGEG-MGSAKGVLLNFEDMGGKLWRFRYSYWNSSQS 244

Query: 244 YVLTKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYIDGRPGGVRAVQVVKLFGVNILEVP 303
           YVLTKGWSRFVKEK+LRAGDVVRFLRSTGPDK+LYIDGR GGV+ VQVVKLFGVNILEVP
Sbjct: 245 YVLTKGWSRFVKEKTLRAGDVVRFLRSTGPDKKLYIDGRAGGVKPVQVVKLFGVNILEVP 304

Query: 304 VGCCGKRETEGLLDLQGSKKQRIIKAL 327
           VG CGKR+ EGL +L+ +KKQRIIKAL
Sbjct: 305 VGWCGKRDREGLSELERTKKQRIIKAL 325

BLAST of Spg020685 vs. ExPASy TrEMBL
Match: A0A6J1IBU1 (AP2/ERF and B3 domain-containing transcription factor RAV1-like OS=Cucurbita maxima OX=3661 GN=LOC111472203 PE=3 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 4.5e-138
Identity = 254/326 (77.91%), Postives = 284/326 (87.12%), Query Frame = 0

Query: 4   SSVDESTASDSTSISPVTPFLFKSSPSPAAVGILHEAESRKLPSSRFKGVVPQPNGRWGA 63
           SS+D+STASDSTS+SPVTP LF SS S +  G+ ++AESRKLPSSRFKGVVPQPNGRWGA
Sbjct: 5   SSLDDSTASDSTSVSPVTPLLFHSSSSSSPPGVPYDAESRKLPSSRFKGVVPQPNGRWGA 64

Query: 64  QIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNFKPDASATATADAAVEADFLK 123
           QIYEKHQRVWLGTFN+EH AAKAYDIAALR+RGRDAVTNFKP   A ++ DAA EADFL 
Sbjct: 65  QIYEKHQRVWLGTFNQEHQAAKAYDIAALRYRGRDAVTNFKP--GAFSSVDAAFEADFLN 124

Query: 124 SHSKLEIVDMLRKHTYNDELQQSKRHRG---AAPAPTSLEQDGSDRGSDCSREVLFEKSV 183
           SHSKLEIVDMLRKHTYNDELQQSKR+ G        T+      +  S+  REVLFEK+V
Sbjct: 125 SHSKLEIVDMLRKHTYNDELQQSKRYGGGTTTTATATAAAHSLHNAASESDREVLFEKTV 184

Query: 184 TPSDVGKLNRLVIPKQHAEKNFPLGDGVVSSAKGMLLNFEDMGGKVWRFRYSYWNSSQSY 243
           TPSDVGKLNRLVIPKQHAEKNFPLG+G + SAKG+LLNFEDMGGK+WRFRYSYWNSSQSY
Sbjct: 185 TPSDVGKLNRLVIPKQHAEKNFPLGEG-MGSAKGVLLNFEDMGGKLWRFRYSYWNSSQSY 244

Query: 244 VLTKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYIDGRPGGVRAVQVVKLFGVNILEVPV 303
           VLTKGWSRFVKEK+LRAGDVVRFLRSTGPDK+LYIDG+ GGV+ VQVV+LFGVNILEVPV
Sbjct: 245 VLTKGWSRFVKEKTLRAGDVVRFLRSTGPDKKLYIDGKAGGVKPVQVVRLFGVNILEVPV 304

Query: 304 GCCGKRETEGLLDLQGSKKQRIIKAL 327
           G CGKR+ EGL +L+ +KKQR+IKAL
Sbjct: 305 GWCGKRDREGLSELERTKKQRMIKAL 327

BLAST of Spg020685 vs. ExPASy TrEMBL
Match: A0A6J1E5P2 (AP2/ERF and B3 domain-containing transcription factor RAV1-like OS=Cucurbita moschata OX=3662 GN=LOC111430186 PE=3 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.5e-130
Identity = 258/334 (77.25%), Postives = 279/334 (83.53%), Query Frame = 0

Query: 1   MDASSVDESTASDSTSISPVTPFLFKSSPSPAAVGILHEAESRKLPSSRFKGVVPQPNGR 60
           MDASS+DES  SDSTSISPVTP LF SSP+P   G+ HEAESRKLPSSRFKGVVPQPNGR
Sbjct: 1   MDASSIDESRGSDSTSISPVTPLLFHSSPAP---GMPHEAESRKLPSSRFKGVVPQPNGR 60

Query: 61  WGAQIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNFKPDASATATADAAVEAD 120
           WGAQIYEKHQRVWLGTFN+EH AA AYDIAALRFRGRDAVTNFK  +        A+E +
Sbjct: 61  WGAQIYEKHQRVWLGTFNQEHHAANAYDIAALRFRGRDAVTNFK--SLDDEDDKDAMEGE 120

Query: 121 FLKSHSKLEIVDMLRKHTYNDELQQSKRHRGA-APAPTSLEQDGSDRGSDCSREVLFEKS 180
           FL SHSKLEIVDMLRKHTYN+EL+QSKR RGA A A T++       G D  REVLFEK+
Sbjct: 121 FLNSHSKLEIVDMLRKHTYNEELEQSKRQRGAIAAANTAVHHS----GVDSKREVLFEKT 180

Query: 181 VTPSDVGKLNRLVIPKQHAEKNFPLGDGVVSSAKGMLLNFEDMGGKVWRFRYSYWNSSQS 240
           VTPSDVGKLNRLVIPKQHAEKNFP+G+G V S KGMLLN EDMGGKVWRFRYSYWNSSQS
Sbjct: 181 VTPSDVGKLNRLVIPKQHAEKNFPMGEG-VGSGKGMLLNLEDMGGKVWRFRYSYWNSSQS 240

Query: 241 YVLTKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYIDGR---PGGVRAVQVVKLFGVNIL 300
           YVLTKGWSRFVKEKSLRAGDVVRFLRSTG DKQLYIDG+    GGV+AV VVKLFGVNIL
Sbjct: 241 YVLTKGWSRFVKEKSLRAGDVVRFLRSTGADKQLYIDGKATSEGGVKAVHVVKLFGVNIL 300

Query: 301 EVPV---GCCGKRETEG-LLDLQGSKKQRIIKAL 327
           EVP+    C G+ + +G LL+LQ SKKQRIIKAL
Sbjct: 301 EVPLPVGRCGGEPKNQGVLLELQPSKKQRIIKAL 324

BLAST of Spg020685 vs. ExPASy TrEMBL
Match: A0A6J1IG08 (AP2/ERF and B3 domain-containing transcription factor RAV1-like OS=Cucurbita maxima OX=3661 GN=LOC111472990 PE=3 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 3.4e-130
Identity = 257/334 (76.95%), Postives = 279/334 (83.53%), Query Frame = 0

Query: 1   MDASSVDESTASDSTSISPVTPFLFKSSPSPAAVGILHEAESRKLPSSRFKGVVPQPNGR 60
           MDASS+DES+ SDSTSISP+TP LF SSP+P   G+ HEAESRKLPSSRFKGVVPQPNGR
Sbjct: 26  MDASSIDESSGSDSTSISPITPLLFHSSPAP---GMPHEAESRKLPSSRFKGVVPQPNGR 85

Query: 61  WGAQIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNFKPDASATATADAAVEAD 120
           WGAQIYEKHQRVWLGTFN+EH AA AYDIAALRFRGRDAVTNFK  +      + A+E +
Sbjct: 86  WGAQIYEKHQRVWLGTFNQEHHAANAYDIAALRFRGRDAVTNFK--SLDDDDDNDAMEGE 145

Query: 121 FLKSHSKLEIVDMLRKHTYNDELQQSKRHRGA-APAPTSLEQDGSDRGSDCSREVLFEKS 180
           FL SHSKLEIVDMLRKHTYN+ELQQSKR RGA A A T +       G D  REVLFEK+
Sbjct: 146 FLNSHSKLEIVDMLRKHTYNEELQQSKRQRGAIAAANTPVHHS----GVDSKREVLFEKT 205

Query: 181 VTPSDVGKLNRLVIPKQHAEKNFPLGDGVVSSAKGMLLNFEDMGGKVWRFRYSYWNSSQS 240
           VTPSDVGKLNRLVIPKQHAEKNFP G+G + S KGMLLN EDMGGKVWRFRYSYWNSSQS
Sbjct: 206 VTPSDVGKLNRLVIPKQHAEKNFPTGEG-LGSGKGMLLNLEDMGGKVWRFRYSYWNSSQS 265

Query: 241 YVLTKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYIDGR---PGGVRAVQVVKLFGVNIL 300
           YVLTKGWSRFVKEKSLRAGDVVRFLRSTG DKQLYIDG+    GGV+AV VVKLFGVNIL
Sbjct: 266 YVLTKGWSRFVKEKSLRAGDVVRFLRSTGADKQLYIDGKAISEGGVKAVHVVKLFGVNIL 325

Query: 301 EVPV---GCCGKRETEG-LLDLQGSKKQRIIKAL 327
           EVP+    C G+ + +G LL+LQ SKKQRIIKAL
Sbjct: 326 EVPLPVGRCGGEPKNQGVLLELQPSKKQRIIKAL 349

BLAST of Spg020685 vs. ExPASy TrEMBL
Match: A0A5D3BWI9 (AP2/ERF and B3 domain-containing transcription factor RAV1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2119G00090 PE=3 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 1.7e-129
Identity = 259/334 (77.54%), Postives = 277/334 (82.93%), Query Frame = 0

Query: 1   MDASSVDESTASDSTSISPVTPFLFKSSPSPAAVGILHEAESRKLPSSRFKGVVPQPNGR 60
           M+ASS+DESTASDSTSISPVTP LF SSPS  A     EAESRKLPSSRFKGVVPQPNGR
Sbjct: 1   MEASSIDESTASDSTSISPVTPLLFHSSPSATA-----EAESRKLPSSRFKGVVPQPNGR 60

Query: 61  WGAQIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNFKPDASATATADAAVEAD 120
           WGAQIYEKHQRVWLGTFNEE+DAAKAYDIAALRFRGRDAVTNFKP  S     D A+EAD
Sbjct: 61  WGAQIYEKHQRVWLGTFNEENDAAKAYDIAALRFRGRDAVTNFKP--SLNHDHDHALEAD 120

Query: 121 FLKSHSKLEIVDMLRKHTYNDELQQSKRHRGAAPAPTS---LEQDGSDRGSDCSREVLFE 180
           FL SHSKLEIVDMLRKHTYN+ELQQSKRH+  A A  S       GS  GSD  REVLFE
Sbjct: 121 FLNSHSKLEIVDMLRKHTYNEELQQSKRHQRGAMAVDSGSFPHYSGSGSGSDSKREVLFE 180

Query: 181 KSVTPSDVGKLNRLVIPKQHAEKNFPLGDGVVSSAKGMLLNFEDMGGKVWRFRYSYWNSS 240
           K+VTPSDVGKLNRLVIPKQHAEKNFP+ +GVV S KGMLLNFEDMGGKVWRFRYSYWNSS
Sbjct: 181 KTVTPSDVGKLNRLVIPKQHAEKNFPMEEGVV-SGKGMLLNFEDMGGKVWRFRYSYWNSS 240

Query: 241 QSYVLTKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYIDGRP---GGVRAVQVVKLFGVN 300
           QSYVLTKGWSRFVK+ +LRAGDVVRFLRSTGPDKQLYI   P    G++ V VVKLFGVN
Sbjct: 241 QSYVLTKGWSRFVKDNTLRAGDVVRFLRSTGPDKQLYIHANPINAPGIKPVHVVKLFGVN 300

Query: 301 ILEVPVGCCGKRETEG-LLDL-QGSKKQRIIKAL 327
           IL++PVG     +TEG  L+L Q +KKQRIIK L
Sbjct: 301 ILQLPVG-----KTEGAFLELQQPTKKQRIIKPL 321

BLAST of Spg020685 vs. TAIR 10
Match: AT1G68840.1 (related to ABI3/VP1 2 )

HSP 1 Score: 350.1 bits (897), Expect = 1.9e-96
Identity = 204/361 (56.51%), Postives = 244/361 (67.59%), Query Frame = 0

Query: 1   MDASSVDESTASDSTSISPVTPFLFKSSPSPAAVGILH-----------------EAESR 60
           MD+S +DE ++S S S S  T    K SP PAA   L+                 E ESR
Sbjct: 1   MDSSCIDEISSSTSESFSATT--AKKLSPPPAAALRLYRMGSGGSSVVLDPENGLETESR 60

Query: 61  KLPSSRFKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNF 120
           KLPSS++KGVVPQPNGRWGAQIYEKHQRVWLGTFNE+ +AA++YDIAA RFRGRDAV NF
Sbjct: 61  KLPSSKYKGVVPQPNGRWGAQIYEKHQRVWLGTFNEQEEAARSYDIAACRFRGRDAVVNF 120

Query: 121 KPDASATATADAAVEADFLKSHSKLEIVDMLRKHTYNDELQQSKRHRGAAPAPTSLEQDG 180
           K   +     D A    FL++HSK EIVDMLRKHTY DEL+Q+ + +       + +++G
Sbjct: 121 K---NVLEDGDLA----FLEAHSKAEIVDMLRKHTYADELEQNNKRQLFLSVDANGKRNG 180

Query: 181 SDRGSD----CSREVLFEKSVTPSDVGKLNRLVIPKQHAEKNFPLGDGVVSSAKGMLLNF 240
           S    +     +REVLFEK+VTPSDVGKLNRLVIPKQHAEK+FPL     +  KG+L+NF
Sbjct: 181 SSTTQNDKVLKTREVLFEKAVTPSDVGKLNRLVIPKQHAEKHFPLPSPSPAVTKGVLINF 240

Query: 241 EDMGGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYIDGR- 300
           ED+ GKVWRFRYSYWNSSQSYVLTKGWSRFVKEK+LRAGDVV F RSTG ++QLYID + 
Sbjct: 241 EDVNGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKNLRAGDVVTFERSTGLERQLYIDWKV 300

Query: 301 ---PGGVRAVQVVKLFGVNILEVP-------VGCCG---KRETEGLLDLQGSKKQRIIKA 327
              P       VV+LFGV+I  V        V  CG    R+ + +  L+ SKKQ II A
Sbjct: 301 RSGPRENPVQVVVRLFGVDIFNVTTVKPNDVVAVCGGKRSRDVDDMFALRCSKKQAIINA 352

BLAST of Spg020685 vs. TAIR 10
Match: AT1G68840.2 (related to ABI3/VP1 2 )

HSP 1 Score: 350.1 bits (897), Expect = 1.9e-96
Identity = 204/361 (56.51%), Postives = 244/361 (67.59%), Query Frame = 0

Query: 1   MDASSVDESTASDSTSISPVTPFLFKSSPSPAAVGILH-----------------EAESR 60
           MD+S +DE ++S S S S  T    K SP PAA   L+                 E ESR
Sbjct: 1   MDSSCIDEISSSTSESFSATT--AKKLSPPPAAALRLYRMGSGGSSVVLDPENGLETESR 60

Query: 61  KLPSSRFKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNF 120
           KLPSS++KGVVPQPNGRWGAQIYEKHQRVWLGTFNE+ +AA++YDIAA RFRGRDAV NF
Sbjct: 61  KLPSSKYKGVVPQPNGRWGAQIYEKHQRVWLGTFNEQEEAARSYDIAACRFRGRDAVVNF 120

Query: 121 KPDASATATADAAVEADFLKSHSKLEIVDMLRKHTYNDELQQSKRHRGAAPAPTSLEQDG 180
           K   +     D A    FL++HSK EIVDMLRKHTY DEL+Q+ + +       + +++G
Sbjct: 121 K---NVLEDGDLA----FLEAHSKAEIVDMLRKHTYADELEQNNKRQLFLSVDANGKRNG 180

Query: 181 SDRGSD----CSREVLFEKSVTPSDVGKLNRLVIPKQHAEKNFPLGDGVVSSAKGMLLNF 240
           S    +     +REVLFEK+VTPSDVGKLNRLVIPKQHAEK+FPL     +  KG+L+NF
Sbjct: 181 SSTTQNDKVLKTREVLFEKAVTPSDVGKLNRLVIPKQHAEKHFPLPSPSPAVTKGVLINF 240

Query: 241 EDMGGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYIDGR- 300
           ED+ GKVWRFRYSYWNSSQSYVLTKGWSRFVKEK+LRAGDVV F RSTG ++QLYID + 
Sbjct: 241 EDVNGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKNLRAGDVVTFERSTGLERQLYIDWKV 300

Query: 301 ---PGGVRAVQVVKLFGVNILEVP-------VGCCG---KRETEGLLDLQGSKKQRIIKA 327
              P       VV+LFGV+I  V        V  CG    R+ + +  L+ SKKQ II A
Sbjct: 301 RSGPRENPVQVVVRLFGVDIFNVTTVKPNDVVAVCGGKRSRDVDDMFALRCSKKQAIINA 352

BLAST of Spg020685 vs. TAIR 10
Match: AT1G13260.1 (related to ABI3/VP1 1 )

HSP 1 Score: 345.9 bits (886), Expect = 3.5e-95
Identity = 211/353 (59.77%), Postives = 240/353 (67.99%), Query Frame = 0

Query: 1   MDASSVDESTAS-----DSTSISPVTPF----LFKSSPSPAAV-----GILHEAESRKLP 60
           M++SSVDEST S     ++ +I+P        L++     + V     G+  EAESRKLP
Sbjct: 1   MESSSVDESTTSTGSICETPAITPAKKSSVGNLYRMGSGSSVVLDSENGV--EAESRKLP 60

Query: 61  SSRFKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNFKPD 120
           SS++KGVVPQPNGRWGAQIYEKHQRVWLGTFNEE +AA+AYD+A  RFR RDAVTNFK  
Sbjct: 61  SSKYKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEDEAARAYDVAVHRFRRRDAVTNFK-- 120

Query: 121 ASATATADAAVEADFLKSHSKLEIVDMLRKHTYNDELQQSKRHRGAAPAPT------SLE 180
                  D   E DFL SHSK EIVDMLRKHTYN+EL+QSKR R      T       L 
Sbjct: 121 -DVKMDED---EVDFLNSHSKSEIVDMLRKHTYNEELEQSKRRRNGNGNMTRTLLTSGLS 180

Query: 181 QDGSDRGSDCSREVLFEKSVTPSDVGKLNRLVIPKQHAEKNFPLGDGVVSSAKGMLLNFE 240
            DG       S E LFEK+VTPSDVGKLNRLVIPK HAEK+FPL    V S KG+LLNFE
Sbjct: 181 NDGVSTTGFRSAEALFEKAVTPSDVGKLNRLVIPKHHAEKHFPLPSSNV-SVKGVLLNFE 240

Query: 241 DMGGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYI--DGR 300
           D+ GKVWRFRYSYWNSSQSYVLTKGWSRFVKEK+LRAGDVV F RS G D+QLYI    R
Sbjct: 241 DVNGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKNLRAGDVVSFSRSNGQDQQLYIGWKSR 300

Query: 301 PGG-VRAVQVVKLFGVNILEVP-----VGCCGKRETEGLLDLQGSKKQRIIKA 326
            G  + A +V++LFGVNI         VG     +TE +L L  SKKQRI  A
Sbjct: 301 SGSDLDAGRVLRLFGVNISPESSRNDVVGNKRVNDTE-MLSLVCSKKQRIFHA 343

BLAST of Spg020685 vs. TAIR 10
Match: AT1G25560.1 (AP2/B3 transcription factor family protein )

HSP 1 Score: 342.0 bits (876), Expect = 5.1e-94
Identity = 210/367 (57.22%), Postives = 238/367 (64.85%), Query Frame = 0

Query: 1   MDASSVDE-STASDSTSIS----PVTPFLFKSSPSPAAVGILH----------------- 60
           M+ S VD+ ST S+S SIS    P T    K S  PA    L+                 
Sbjct: 1   MEYSCVDDSSTTSESLSISTTPKPTTTTEKKLSSPPATSMRLYRMGSGGSSVVLDSENGV 60

Query: 61  EAESRKLPSSRFKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRD 120
           E ESRKLPSS++KGVVPQPNGRWGAQIYEKHQRVWLGTFNEE +AA +YDIA  RFRGRD
Sbjct: 61  ETESRKLPSSKYKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEEEAASSYDIAVRRFRGRD 120

Query: 121 AVTNFKPDASATATADAAVEADFLKSHSKLEIVDMLRKHTYNDELQQSKRHRGAAPAPTS 180
           AVTNFK         DA  E+ FL +HSK EIVDMLRKHTY DE +QS+R         S
Sbjct: 121 AVTNFKSQVDGN---DA--ESAFLDAHSKAEIVDMLRKHTYADEFEQSRRKFVNGDGKRS 180

Query: 181 LEQDGSDRGSDC---SREVLFEKSVTPSDVGKLNRLVIPKQHAEKNFPLGDGVV------ 240
              + +  G+D    +REVLFEK+VTPSDVGKLNRLVIPKQHAEK+FPL           
Sbjct: 181 -GLETATYGNDAVLRAREVLFEKTVTPSDVGKLNRLVIPKQHAEKHFPLPAMTTAMGMNP 240

Query: 241 SSAKGMLLNFEDMGGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKSLRAGDVVRFLRSTGP 300
           S  KG+L+N ED  GKVWRFRYSYWNSSQSYVLTKGWSRFVKEK+LRAGDVV F RSTGP
Sbjct: 241 SPTKGVLINLEDRTGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKNLRAGDVVCFERSTGP 300

Query: 301 DKQLYIDGRPGGVRAVQVVKLFGVNIL--------EVPVGCCGKRET--EGLLDLQGSKK 327
           D+QLYI  +        VV+LFGVNI         +V V C GK+ +  + L  L  SKK
Sbjct: 301 DRQLYIHWKVRSSPVQTVVRLFGVNIFNVSNEKPNDVAVECVGKKRSREDDLFSLGCSKK 360

BLAST of Spg020685 vs. TAIR 10
Match: AT3G25730.1 (ethylene response DNA binding factor 3 )

HSP 1 Score: 318.5 bits (815), Expect = 6.1e-87
Identity = 196/342 (57.31%), Postives = 228/342 (66.67%), Query Frame = 0

Query: 1   MDA-SSVDE-STASDS----TSISPVTPFLFKSS------PSPAAVGILHEAESRKLPSS 60
           MDA SSVDE ST +DS     S SP +      S       S   V +  EAESRKLPSS
Sbjct: 1   MDAMSSVDESSTTTDSIPARKSSSPASLLYRMGSGTSVVLDSENGVEVEVEAESRKLPSS 60

Query: 61  RFKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEHDAAKAYDIAALRFRGRDAVTNFKPDAS 120
           RFKGVVPQPNGRWGAQIYEKHQRVWLGTFNEE +AA+AYD+AA RFRGRDAVTNFK    
Sbjct: 61  RFKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEDEAARAYDVAAHRFRGRDAVTNFKDTTF 120

Query: 121 ATATADAAVEADFLKSHSKLEIVDMLRKHTYNDELQQSKRHRGAAPAPTSLEQDGS--DR 180
                    E +FL +HSK EIVDMLRKHTY +EL Q KR+R      T+     S    
Sbjct: 121 EE-------EVEFLNAHSKSEIVDMLRKHTYKEELDQRKRNRDGNGKETTAFALASMVVM 180

Query: 181 GSDCSREVLFEKSVTPSDVGKLNRLVIPKQHAEKNF--PLGDGVVSSAKGMLLNFEDMGG 240
               + E+LFEK+VTPSDVGKLNRLVIPK  AEK+F  PLG+  V S KGMLLNFED+ G
Sbjct: 181 TGFKTAELLFEKTVTPSDVGKLNRLVIPKHQAEKHFPLPLGNNNV-SVKGMLLNFEDVNG 240

Query: 241 KVWRFRYSYWNSSQSYVLTKGWSRFVKEKSLRAGDVVRFLRSTGPDKQLYIDGRPGG--- 300
           KVWRFRYSYWNSSQSYVLTKGWSRFVKEK L AGD++ F RS   D++ +I  +      
Sbjct: 241 KVWRFRYSYWNSSQSYVLTKGWSRFVKEKRLCAGDLISFKRSNDQDQKFFIGWKSKSGLD 300

Query: 301 VRAVQVVKLFGVNILEVPVGCCGKRETEGLLDLQGSKKQRII 324
           +   +V++LFGV+I  +      K  TE L+     KKQR++
Sbjct: 301 LETGRVMRLFGVDI-SLNAVVVVKETTEVLMSSLRCKKQRVL 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023541268.13.7e-13978.77AP2/ERF and B3 domain-containing transcription factor RAV1-like [Cucurbita pepo ... [more]
KAG6601748.14.9e-13979.08AP2/ERF and B3 domain-containing transcription factor ARF14, partial [Cucurbita ... [more]
KAG7032471.16.4e-13979.01AP2/ERF and B3 domain-containing transcription repressor TEM1, partial [Cucurbit... [more]
XP_022930067.18.4e-13979.20AP2/ERF and B3 domain-containing transcription factor RAV1-like [Cucurbita mosch... [more]
XP_022973620.19.2e-13877.91AP2/ERF and B3 domain-containing transcription factor RAV1-like [Cucurbita maxim... [more]
Match NameE-valueIdentityDescription
P822802.6e-9556.51AP2/ERF and B3 domain-containing transcription repressor RAV2 OS=Arabidopsis tha... [more]
Q9ZWM95.0e-9459.77AP2/ERF and B3 domain-containing transcription factor RAV1 OS=Arabidopsis thalia... [more]
Q9C6M57.2e-9357.22AP2/ERF and B3 domain-containing transcription repressor TEM1 OS=Arabidopsis tha... [more]
Q9LS068.5e-8657.31AP2/ERF and B3 domain-containing transcription factor ARF14 OS=Arabidopsis thali... [more]
Q8RZX98.0e-8457.81AP2/ERF and B3 domain-containing protein Os01g0693400 OS=Oryza sativa subsp. jap... [more]
Match NameE-valueIdentityDescription
A0A6J1EPB74.0e-13979.20AP2/ERF and B3 domain-containing transcription factor RAV1-like OS=Cucurbita mos... [more]
A0A6J1IBU14.5e-13877.91AP2/ERF and B3 domain-containing transcription factor RAV1-like OS=Cucurbita max... [more]
A0A6J1E5P21.5e-13077.25AP2/ERF and B3 domain-containing transcription factor RAV1-like OS=Cucurbita mos... [more]
A0A6J1IG083.4e-13076.95AP2/ERF and B3 domain-containing transcription factor RAV1-like OS=Cucurbita max... [more]
A0A5D3BWI91.7e-12977.54AP2/ERF and B3 domain-containing transcription factor RAV1-like OS=Cucumis melo ... [more]
Match NameE-valueIdentityDescription
AT1G68840.11.9e-9656.51related to ABI3/VP1 2 [more]
AT1G68840.21.9e-9656.51related to ABI3/VP1 2 [more]
AT1G13260.13.5e-9559.77related to ABI3/VP1 1 [more]
AT1G25560.15.1e-9457.22AP2/B3 transcription factor family protein [more]
AT3G25730.16.1e-8757.31ethylene response DNA binding factor 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003340B3 DNA binding domainSMARTSM01019B3_2coord: 176..281
e-value: 1.8E-23
score: 94.0
IPR003340B3 DNA binding domainPFAMPF02362B3coord: 176..270
e-value: 1.9E-26
score: 91.8
IPR003340B3 DNA binding domainPROSITEPS50863B3coord: 176..279
score: 13.662183
IPR003340B3 DNA binding domainCDDcd10017B3_DNAcoord: 175..263
e-value: 7.41513E-28
score: 102.406
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 49..110
e-value: 1.8E-28
score: 110.6
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 48..96
e-value: 7.9E-8
score: 32.4
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 49..104
score: 19.95434
IPR001471AP2/ERF domainCDDcd00018AP2coord: 48..103
e-value: 6.68661E-26
score: 96.1866
IPR015300DNA-binding pseudobarrel domain superfamilyGENE3D2.40.330.10coord: 165..282
e-value: 5.3E-43
score: 147.5
IPR015300DNA-binding pseudobarrel domain superfamilySUPERFAMILY101936DNA-binding pseudobarrel domaincoord: 174..268
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 48..105
e-value: 1.6E-20
score: 75.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 141..169
NoneNo IPR availablePANTHERPTHR31140:SF1AP2/ERF AND B3 DOMAIN-CONTAINING TRANSCRIPTION REPRESSOR TEM1coord: 37..301
IPR044800B3 domain-containing transcription factor LEC2-likePANTHERPTHR31140B3 DOMAIN-CONTAINING TRANSCRIPTION FACTOR ABI3coord: 37..301
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 48..105

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg020685.1Spg020685.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity