Spg019971 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg019971
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RING/U-box superfamily protein
Location	scaffold5: 36936788 .. 36942862 (+)
RNA-Seq Expression	Spg019971
Synteny	Spg019971

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAATTATAGAAAGAACCCAACGAAGACAAAGACCGAGTGAAGGAGAACGCCAACAAGAACCCCATAAACAGAAACCCACTTTCCTTTAATCTTACGGCATCAAATTAGCAAATAGTAACTGAAGATTCTTCCTTTTTCTCTCTTTCTTCTGATTCATCTTACTCTCTCTGAAAATCCCAATTAATGGATCCACCCGTTTTCTAGAGAGAGAGACTGAGTGAGGAGAGAGAAATTTGGGGCACTGGTTTTGTGCTCATTCTTGCAGTTTGTCAGATTAGGGTTTTTTAATATCATTTTTCAGTCCCCAGAATTCCCTTCACATGAGGTGAATTTTTTCATGGTATGGAACCTTCATTCTTTTCTTTGCCCATGTTTTTTTAGAATACTCTTTTTTTTTTCTTTTTTGTGTATGTTCCCATTTCTTCAGTCTCCAACCCCCAATCCCGAAATTTGTGTAAATTTTTATTTGGTGCTAATAAATGGGGTTTGATGGATTGGTTAGTAGGTTAGTGGGTCGATCTTGATTTGCACAGAAAATCCAAGAACTATTTGTTTTATTTACATTTTCTTCGATTCGGACATGTTCCTCTTTTTAGTATTCGGTTTTTGGAATTTGGTGTCCTCGATGTGGAAATCTTACGAATCTTAATCCTTTCGGGCTGATTCTACGATGGTTATTCTGTCGCTTTTTGATCTAGAATATAGACTTGAAAGAAATTTCCGAGTGATGTAGATCGTTTTCTGTGATTTTTAGGGGCGATTGCTGCTTCAATTTGGGAATTAGGGAAAGTTTGCTTCAGAGATTCTCTTTTGGTTAACGTTTGGAAATTTGGAGTCCTCGATCAGCGGTGGATATGAGCTTCTTCACGTGATTCTTTTGAGTAATTAAGAAGAGCGAAGGACGGTGTTAAATTCATTTGCCGGTGGGTCATCTCTAATTTGCTGCCACTTTAATTTAAAACCTTCTGGGATTTGTGGGTCGTTTACAAAATTGTGAGAATGGAGCCTCGTGAGATTTTTGGTCGTGGGTCTGCTCATGGTCCAGCTGAGGAAGATGATGTTGAAGATTTTCGAAGTTGTTGTGAAGATCACGAGGTATGGAAAGAGACCGAGGATGTAGTAGACGAAGGGTCTGAGGAGGAGGAACTTGATGAATTTTCAGTAAAAATGTTCTTTAAGGGTGTTTCAATAGCTGAGATTGGTGACCCTGCTTCGGGTCTTTCTGGTATTGGAGTATTCATGGAAAAATCAGGGAAAGTCCCTTTTATTCAGGTCCAGAAAAAGCTTGATTTTTATGTTGATGAGTTAGTTGCTGATTATTTAGCTCTAATGGATGGTCTGGTGGAGGCCATGCAGAATGACATTCGCTGTATTCGAGCTTTCACTGACTCCGATCTGTTATATAATCAGGTTATTATTTCCCCTATCTGCCTAAACCTTGCGTGTTTTGATTTATGCTAATTTTCATTTTTATTTCTATATCATACTCATTTTTGAAAGTTTGTTGCTGTTTGTTGACTCTATTTTTGGGGTTCTTTTGCATTGTTTTCAAAAGAAAAATGGAGAATTGTTCTCACTCCTACTCTCAGTAATGTGGTTTTCTGGAAACTCCAGGCTTTTTAACATTTGACTAGTTGTATTTGATTTTCTTTATAAGAATTGAATTTATTGATGAAATATAATTTGTTGAGAAAGGGGAGGGGATAGAATTGATTATTACCATTTCTTCTGCACAAGTCAGAGGAGTATGGGGTTGGTTACATGTTTCACGGCCAGTAATTTGAGTGTTGGGACTTTATGAGCTGTGGAAGTCGTGGAGAGTGGAGAGATGCATAAACTCTTACAGTTTTCTAGCCATGCGCTTACAAGTTTTTCTTATATGCTTAAGATAATGTGAATGTTTGACAGATAACAAATGAAGAAGAGCCTGAAATTCCACTTCTTGTAGCGCTTAAAGAAAGGATTCTAGAACATGCTAGCACTTTTGAAGCCTTTTCCCTGAATCTTTCTTCAAGTACTGATCTTGAGCAGCCATTTCATCTTGCCAAAGTGGCCATTGGGGTTCTTTCTTGTCCGCAAAAGGGAGATAAATCAGTTGAAAATTGTTCTATTTGTTGTGAGAACAAACCATCTGCTATGATGGTTGCATTGAAGTGTTGTCACAAGTTCTGCTCTCATTGTATGAAGACTTATGTTGATGGAAAATTAGAGACATCTCAAGTTCCTATCAGATGCCCTCAGTTGAGATGCAAATATTACATCAAAAGCAATGAGTTTAAATCTTTCCTTCCACTGACTTTATATGAATTGTTAGAGACTACCTTCGCCGAAGCAAATATTCAATCAGATAGAATTTACTGCCCTTTTCCAAATTGCTCTGTCCTGTTAGACCCCACCGAATGTTTGTCAGCTCGAGCAAGTTCATCAAGTCAATCAGATAATAGCTGTATTGAGTGCCCAGTTTGTCAGAGATTTATCTGTGTTGAATGTCGTGTTCCTTGGCACTCATCCATGAGTTGTGAAGAGTTTCAGAACATTCCATTAGATGAGAGAGACACTGCAGATATTACCTTGCATCGTCTTGCACAAAACAAAAGGTGGAGGCGTTGTCAGCAGTGTCGTAGAATGATTGAATTGTCTCAAGGTTGCTACCATATGACTTGCTGGTAAGGCTACTTCCTTCATATCTCTCTTTATTTCTATTAACCTGTTTGTTTATGAGATATTGTGGAAGCACCATCATCAGAAATATATATCACAAGTTTAGTTGGATGTTAGATTTTTAAGGTTTTGGACCTTTGGTTGTAGGACGATTTGAAAATTTGGTGCACTTTATATGCTTATGTAGTTATGTGGACAATCAACAGGCATGGCATACAAATAGAATCTTCCCTCCCCATTTTAATGTCTTTGTTTTCTGCTAGATTAGATATTTTATTGTTTTACAAATTTTCTAGCGACCATCATTTACTATTTAGTCTATCTCCCTGAGGAACTTCTGTTTATCCCAAACTCTACTGGTTTACTAATCTCATGGGCTACCTAAATTCAGGCTGCTAGAATTCACCTATTAGGCTATTTACTTACTTATCACAAATCCACAGATTAATTACATGTTGGAACTTGGAACTCATTTCTCTAGTCTACGCAATTCAAACACATACTACATAAACCTAAACTTCTCGCCTCAAGCTTACCAAACCCAAGTTTATTGGAATTGCTAGCCAAATATTCCCATAGATTTTTATGTCAATACAGTGCCTATACGGTAGGGAAAATCTTAATTTGGTGCATGTGTTATAACAGCCTTATTGTTCAATGTGATGAACATAATTGATGTATGCATCACAAGTAGGTCTAACTTATTTGAAGAAATGAAATGTCGGGGAACACATGATAGTGCTAAAATATCATTTGCCATTATGTATTGTGCAGTCTAAGTTGGTCATACAATGATTACAGCCTTTCTAGGGATTTCTCTAGAGATGGAACCCCTCCTCTTAAATTAATTATAGTTTGAAAATATTAACGGTTGCTGTGGTAAAGAGATGAAATATTAGCTCAACTTTAAATGTTTCTACTTGTCTTCAGAGTTCCTCCATCCGTCGTTTTTGGGGCAAGCTAGGATGTGTGCTCTGTTATTGAGTCTATGGGGGGAGAGAAATAATAGAGTGTTTAGAGGGATAGAGAGAGGTCCGTGTGATATGTGGTCCCTTTCGAGGTTCCATGTATCCCTATGGCCATCATCAATTACGAAATTATTGTGTAATTACTCGTTAGGTCTCATTTTACTTGATTGGAGTCTCTTCCTTCAATTGGACTCCTTTTTGTAGGCTTGTTTTTTTGTATGTTGGCTTATTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTGCTTGTCTTGTAATTTTTTCATATCTTTTGGGTGTTGAACATGATGAAAGGGATTTTCTTATTTTTGCTTTTCCAACTTTTAACAGTTTCTGGCTGTTTCCTTTTAATTTCACATTCAAACTCTTATCCAAGAGTCTGTCAAGCACGCTACTATTAGATCAACCAAATGTTTGTGCACACTGTATTCTTGTTGTCACTCACACATGGCTAAATGTCAGTCAACGTAATATTGAGTCATATCTTATTCCTTCCTCATTACTGGACTTTGGACTTTGCGTACAAGTTGATCGTTGTGTGAATTATAATAGCTTATCAGTGAATAATATTACATATATACCCCAGCCACCCCCCCCCCCCCCCCCCCCCAATGAGGGGCTGGATTCGAGTCTTTTACATGTGTAATCGTTTCAAGAAATACCTTCCACAGATGCATTTCTCCTGCTCATTAGTTGCATATTTAATCTGCCCATGAAACTCTTTTTTCTCACACTACCAATCTAGAGTCGTCTTTTTAATTTTGTTGCACACAGTTCTCCTAGTAGGTTCTAAAGTTTAGATCCCAACACTTGGATGATGAATCCAAGGTCTTTTTATTCCAAAACCATTGATTTACATTCTTTGTCCATTTAGTGAATATAATTCCTGTCTGGGTGTTTGGCGAGTCGAGATCTTCTCTTACTACCAAGCTAGAGCTGTCTTGCTAATTTTATTGCACACATTTCTCCTAGTAGGTTCTAAAGTTTAGAACCCAACACTTGGTTGGATGATGTATCCGACGTCTTTTATTCTAAACCCATTGTTTTACATTCTTTGTTCATTTAGTGACTATTATTCCTGTGTGAGTGTTTGGTGAATTGAGATCTTTGAACGTGCACTAGCACTACTGTCACATACATTTGTGGTCAAGCGAATGAGATTCAATTTTAGCAAAACATGATCTGCAAATGCCAAATTGCAATTCTTGGTTTCTAGTTGGTGCAGGGCTTTATGTATCGGACTTTAATGTTTATACTGCTTTGCTTAATATGTTTACAGGTGCGGACACGAATTCTGCTATTCTTGTGGTGCTGAGTACCAAGATGGGCAACAGACCTGCCAATGCGCCTTTTGGGACGACGACAATTCCGAGGACTTGGTCACCCACTCCATCCAAGAATCTGAGCAGTGGGCATGGGAGACTTTTAATTCCCTTCCCATGATAATGGATGCATACTCAGAACAAGAGAGGTCACAATTAGCATTAATTCAACGATTCCTCGCAGGGGGATTTAGTTTGAGCGACCACCAACCATATCAATCTCCACCACGATGTACAGACTCTTATGTCGACACGATGAAGGACCTACACCAGCTTCCTTGGCTCGAGAGGTTTGTTTCTGTGATAAGCGATAGCTACTACGAAGATTATGTACAATGATATCAATTTTTAGGTGCCTTACTGAAGTTGAAAAATAAAAGGGAGGTAAAAGAGTTCCTCACTCTAGTTTTCCTCCCTCATTTACACCGCATGGCTTGGCGTTTCGATCCTTAGTTAAGCTCTCTTTATCTAGAAATTATGATGTGACCCTCAAAGGTCCTCCTTGCATTAGATTAGAATGAAGGAATCTTATGCAAGAATTTCAGCTTTATTTCTTTCCTCTTTCAAAGAAATGTTTGAGAAAAAAAAAAAAAAAGGGAAAAGACAAAAAGAAATAGAGAACAATTTAATGATGTCAGTCAATGAAAATGAAAATTGTCTTCATTCTGTTGTATGGATGTGCAATTTTACCTGCAATCCTTTCTCATTGCAGAGGTAGGGTTTTTGTCATTTTTAAATGGTCCCAATTTCATTTCTTACATGATTGTGCTGCACATTTGGATGCTGTATTGTTCTTAGCTGCAGGTTGAATTCAGTTGGGTCAGTTTTGGACTCATCAGGAGTCACTTTTACTTTTTTTTTTTTTTTTCTTTTTTGGAAAAGTTAAAATCTCTTCTGCCCAAAATTTTAAACCGATAACATCCTGTTCGAACAATAATGAGGAGATGAGGATTCAAACCTCTAACTTTTTGGTCGAGGATATAATGCTTTAACTAGTTGAGTTATACTCAAGTTAGCAATCCAATGGTACTTTGAGAGTATTGTTTTGATATTTTGCTATTTTGCTGCATACCATTTGGATGAGAGAGTTCTTTCTCTAGTC

mRNA sequence

ATGGAGCCTCGTGAGATTTTTGGTCGTGGGTCTGCTCATGGTCCAGCTGAGGAAGATGATGTTGAAGATTTTCGAAGTTGTTGTGAAGATCACGAGGTATGGAAAGAGACCGAGGATGTAGTAGACGAAGGGTCTGAGGAGGAGGAACTTGATGAATTTTCAGTAAAAATGTTCTTTAAGGGTGTTTCAATAGCTGAGATTGGTGACCCTGCTTCGGGTCTTTCTGGTATTGGAGTATTCATGGAAAAATCAGGGAAAGTCCCTTTTATTCAGGTCCAGAAAAAGCTTGATTTTTATGTTGATGAGTTAGTTGCTGATTATTTAGCTCTAATGGATGGTCTGGTGGAGGCCATGCAGAATGACATTCGCTGTATTCGAGCTTTCACTGACTCCGATCTGTTATATAATCAGATAACAAATGAAGAAGAGCCTGAAATTCCACTTCTTGTAGCGCTTAAAGAAAGGATTCTAGAACATGCTAGCACTTTTGAAGCCTTTTCCCTGAATCTTTCTTCAAGTACTGATCTTGAGCAGCCATTTCATCTTGCCAAAGTGGCCATTGGGGTTCTTTCTTGTCCGCAAAAGGGAGATAAATCAGTTGAAAATTGTTCTATTTGTTGTGAGAACAAACCATCTGCTATGATGGTTGCATTGAAGTGTTGTCACAAGTTCTGCTCTCATTGTATGAAGACTTATGTTGATGGAAAATTAGAGACATCTCAAGTTCCTATCAGATGCCCTCAGTTGAGATGCAAATATTACATCAAAAGCAATGAGTTTAAATCTTTCCTTCCACTGACTTTATATGAATTGTTAGAGACTACCTTCGCCGAAGCAAATATTCAATCAGATAGAATTTACTGCCCTTTTCCAAATTGCTCTGTCCTGTTAGACCCCACCGAATGTTTGTCAGCTCGAGCAAGTTCATCAAGTCAATCAGATAATAGCTGTATTGAGTGCCCAGTTTGTCAGAGATTTATCTGTGTTGAATGTCGTGTTCCTTGGCACTCATCCATGAGTTGTGAAGAGTTTCAGAACATTCCATTAGATGAGAGAGACACTGCAGATATTACCTTGCATCGTCTTGCACAAAACAAAAGGTGGAGGCGTTGTCAGCAGTGTCGTAGAATGATTGAATTGTCTCAAGGTTGCTACCATATGACTTGCTGGTGCGGACACGAATTCTGCTATTCTTGTGGTGCTGAGTACCAAGATGGGCAACAGACCTGCCAATGCGCCTTTTGGGACGACGACAATTCCGAGGACTTGGTCACCCACTCCATCCAAGAATCTGAGCAGTGGGCATGGGAGACTTTTAATTCCCTTCCCATGATAATGGATGCATACTCAGAACAAGAGAGGTCACAATTAGCATTAATTCAACGATTCCTCGCAGGGGGATTTAGTTTGAGCGACCACCAACCATATCAATCTCCACCACGATGTACAGACTCTTATGTCGACACGATGAAGGACCTACACCAGCTTCCTTGGCTCGAGAGGTTTGTTTCTGTGATAAGCGATAGCTACTACGAAGATTATGTACAATGA

Coding sequence (CDS)

ATGGAGCCTCGTGAGATTTTTGGTCGTGGGTCTGCTCATGGTCCAGCTGAGGAAGATGATGTTGAAGATTTTCGAAGTTGTTGTGAAGATCACGAGGTATGGAAAGAGACCGAGGATGTAGTAGACGAAGGGTCTGAGGAGGAGGAACTTGATGAATTTTCAGTAAAAATGTTCTTTAAGGGTGTTTCAATAGCTGAGATTGGTGACCCTGCTTCGGGTCTTTCTGGTATTGGAGTATTCATGGAAAAATCAGGGAAAGTCCCTTTTATTCAGGTCCAGAAAAAGCTTGATTTTTATGTTGATGAGTTAGTTGCTGATTATTTAGCTCTAATGGATGGTCTGGTGGAGGCCATGCAGAATGACATTCGCTGTATTCGAGCTTTCACTGACTCCGATCTGTTATATAATCAGATAACAAATGAAGAAGAGCCTGAAATTCCACTTCTTGTAGCGCTTAAAGAAAGGATTCTAGAACATGCTAGCACTTTTGAAGCCTTTTCCCTGAATCTTTCTTCAAGTACTGATCTTGAGCAGCCATTTCATCTTGCCAAAGTGGCCATTGGGGTTCTTTCTTGTCCGCAAAAGGGAGATAAATCAGTTGAAAATTGTTCTATTTGTTGTGAGAACAAACCATCTGCTATGATGGTTGCATTGAAGTGTTGTCACAAGTTCTGCTCTCATTGTATGAAGACTTATGTTGATGGAAAATTAGAGACATCTCAAGTTCCTATCAGATGCCCTCAGTTGAGATGCAAATATTACATCAAAAGCAATGAGTTTAAATCTTTCCTTCCACTGACTTTATATGAATTGTTAGAGACTACCTTCGCCGAAGCAAATATTCAATCAGATAGAATTTACTGCCCTTTTCCAAATTGCTCTGTCCTGTTAGACCCCACCGAATGTTTGTCAGCTCGAGCAAGTTCATCAAGTCAATCAGATAATAGCTGTATTGAGTGCCCAGTTTGTCAGAGATTTATCTGTGTTGAATGTCGTGTTCCTTGGCACTCATCCATGAGTTGTGAAGAGTTTCAGAACATTCCATTAGATGAGAGAGACACTGCAGATATTACCTTGCATCGTCTTGCACAAAACAAAAGGTGGAGGCGTTGTCAGCAGTGTCGTAGAATGATTGAATTGTCTCAAGGTTGCTACCATATGACTTGCTGGTGCGGACACGAATTCTGCTATTCTTGTGGTGCTGAGTACCAAGATGGGCAACAGACCTGCCAATGCGCCTTTTGGGACGACGACAATTCCGAGGACTTGGTCACCCACTCCATCCAAGAATCTGAGCAGTGGGCATGGGAGACTTTTAATTCCCTTCCCATGATAATGGATGCATACTCAGAACAAGAGAGGTCACAATTAGCATTAATTCAACGATTCCTCGCAGGGGGATTTAGTTTGAGCGACCACCAACCATATCAATCTCCACCACGATGTACAGACTCTTATGTCGACACGATGAAGGACCTACACCAGCTTCCTTGGCTCGAGAGGTTTGTTTCTGTGATAAGCGATAGCTACTACGAAGATTATGTACAATGA

Protein sequence

MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVDEGSEEEELDEFSVKMFFKGVSIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQNDIRCIRAFTDSDLLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSSTDLEQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKTYVDGKLETSQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLETTFAEANIQSDRIYCPFPNCSVLLDPTECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITLHRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDNSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ

Homology

[Close]

BLAST of Spg019971 vs. NCBI nr
Match: XP_008459101.1 (PREDICTED: E3 ubiquitin-protein ligase arih1 [Cucumis melo] >XP_008459102.1 PREDICTED: E3 ubiquitin-protein ligase arih1 [Cucumis melo] >KAA0043320.1 E3 ubiquitin-protein ligase arih1 [Cucumis melo var. makuwa] >TYK29385.1 E3 ubiquitin-protein ligase arih1 [Cucumis melo var. makuwa])

HSP 1 Score: 1003.0 bits (2592), Expect = 9.1e-289
Identity = 475/516 (92.05%), Postives = 500/516 (96.90%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVDEGSEEEELDEFSVKMFFK 60
           ME R+IFGRGS+HGPAEEDDVE+FRSCCEDHEVWKETED V+E S EE+LDEFSVKMFFK
Sbjct: 1   MEHRQIFGRGSSHGPAEEDDVEEFRSCCEDHEVWKETEDAVEEES-EEDLDEFSVKMFFK 60

Query: 61  GVSIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQN 120
           GVSIAEIGDPASGLSGIGVFMEKSGK+PF+QVQKKLDFYVDELVADYLALMDGLVEAMQN
Sbjct: 61  GVSIAEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQN 120

Query: 121 DIRCIRAFTDSDLLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSSTDLEQPF 180
           D+RCIRAFTDSDLLY QITNEEEPEIPLLVALKERILEHAS FE+FSL LSSS DLEQPF
Sbjct: 121 DVRCIRAFTDSDLLYKQITNEEEPEIPLLVALKERILEHASNFESFSLILSSSADLEQPF 180

Query: 181 HLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKTYVDGKLETS 240
           HLAKVAIGVLSCPQKGDKSVENCSICC+NKPSAMMVALKCCHKFCS C+KTYVDGK++ S
Sbjct: 181 HLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCLKTYVDGKVDAS 240

Query: 241 QVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLETTFAEANIQSDRIYCPFPNCSVLLDPT 300
           QVPIRCPQLRCKYYI SNEFKSFLPLTLYELLE T AEANI +DRIYCPFPNCSVLLDP+
Sbjct: 241 QVPIRCPQLRCKYYITSNEFKSFLPLTLYELLENTLAEANIHADRIYCPFPNCSVLLDPS 300

Query: 301 ECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITLH 360
           ECLSARASSSSQSDNSCIECPVCQRFICVEC+VPWHSS+SCEEFQNIPL+ERDTADITLH
Sbjct: 301 ECLSARASSSSQSDNSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLH 360

Query: 361 RLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDNS 420
           RLAQNKRWRRCQ+C RMIEL+QGC+HMTCWCGHEFCYSCG EYQDGQQTCQCAFWD+DNS
Sbjct: 361 RLAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWDEDNS 420

Query: 421 EDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPP 480
           E+LVTHS+QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPP
Sbjct: 421 ENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPP 480

Query: 481 RCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 517
           RCTDSYVDTMKD+HQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 RCTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

[Close]

BLAST of Spg019971 vs. NCBI nr
Match: XP_022142466.1 (uncharacterized protein LOC111012584 [Momordica charantia] >XP_022142467.1 uncharacterized protein LOC111012584 [Momordica charantia] >XP_022142468.1 uncharacterized protein LOC111012584 [Momordica charantia] >XP_022142469.1 uncharacterized protein LOC111012584 [Momordica charantia])

HSP 1 Score: 995.3 bits (2572), Expect = 1.9e-286
Identity = 479/517 (92.65%), Postives = 495/517 (95.74%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVDEGSEEEE-LDEFSVKMFF 60
           ME REIFGRGS+HGPAEEDDVEDFRSCCEDHEVWKE ED+V+EGSEEEE LDEFSVKMFF
Sbjct: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKEAEDLVEEGSEEEEHLDEFSVKMFF 60

Query: 61  KGVSIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KG+SIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGISIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIRCIRAFTDSDLLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSSTDLEQP 180
           N+IRCIRAFTDSDLLYNQITNEEE EIPLL+ALKERILEHASTFEAFSLNLSSSTDLEQP
Sbjct: 121 NEIRCIRAFTDSDLLYNQITNEEESEIPLLIALKERILEHASTFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKTYVDGKLET 240
           FHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMV LKCCHKFCSHCMKTYVDGK+++
Sbjct: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVGLKCCHKFCSHCMKTYVDGKVQS 240

Query: 241 SQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLETTFAEANIQSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRCKYYI  NEF+SFLPLTLYE LE T AEANI SDRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITINEFRSFLPLTLYESLENTLAEANIHSDRIYCPFPNCSVLLDP 300

Query: 301 TECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITL 360
           +ECLS RASSSSQSDNSCIECPVCQRFICV C VPWHSSMSCEEFQNI     DT DITL
Sbjct: 301 SECLSGRASSSSQSDNSCIECPVCQRFICVYCGVPWHSSMSCEEFQNI-----DTEDITL 360

Query: 361 HRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDN 420
           HRLAQNKRW+RCQQCRRMIEL+QGCYHMTCWCGHEFCYSCGAEYQD QQTCQCA WD++N
Sbjct: 361 HRLAQNKRWKRCQQCRRMIELTQGCYHMTCWCGHEFCYSCGAEYQDSQQTCQCALWDEEN 420

Query: 421 SEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQP+QSP
Sbjct: 421 SEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPHQSP 480

Query: 481 PRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 517
           PRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 PRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 512

[Close]

BLAST of Spg019971 vs. NCBI nr
Match: XP_004145453.1 (E3 ubiquitin-protein ligase arih1 [Cucumis sativus] >XP_011660321.1 E3 ubiquitin-protein ligase arih1 [Cucumis sativus] >KGN66894.1 hypothetical protein Csa_007170 [Cucumis sativus])

HSP 1 Score: 988.8 bits (2555), Expect = 1.8e-284
Identity = 469/516 (90.89%), Postives = 494/516 (95.74%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVDEGSEEEELDEFSVKMFFK 60
           ME REIFGRGS+HGPAEEDDVEDFRSCCEDHEVWKETED V+E S EE+LDEFSVKMFFK
Sbjct: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEES-EEDLDEFSVKMFFK 60

Query: 61  GVSIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQN 120
           GVSIAEIGD ASGLSGIGV MEKSGK+PF+QVQKKLDFYVDELVADYLALMDGLVEAMQN
Sbjct: 61  GVSIAEIGDSASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQN 120

Query: 121 DIRCIRAFTDSDLLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSSTDLEQPF 180
           D+RCIRAFT SDLLY QITN EEPEIPLLVALKERILEHAS FE+FSL+LSS  DL+QPF
Sbjct: 121 DVRCIRAFTSSDLLYKQITNHEEPEIPLLVALKERILEHASNFESFSLSLSSGADLQQPF 180

Query: 181 HLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKTYVDGKLETS 240
           HLAKVAIGVLSCPQKGDKSVENCSICC+NKPSAMMVALKCCHKFCS CMKTYVDGK++ S
Sbjct: 181 HLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDAS 240

Query: 241 QVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLETTFAEANIQSDRIYCPFPNCSVLLDPT 300
           QVPIRCPQLRCKYYI SNEFKSFLPLTLYE LE T AEANI +DRIYCPFPNCSVLLDP+
Sbjct: 241 QVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDPS 300

Query: 301 ECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITLH 360
           ECLSARASSSSQS+NSCIECPVCQRFICVEC+VPWHSS+SCEEFQNIPL+ERDTADITLH
Sbjct: 301 ECLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLH 360

Query: 361 RLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDNS 420
           RLAQNKRWRRCQ+C RMIEL+QGC+HMTCWCGHEFCYSCG EYQDGQQTCQCAFWD+DNS
Sbjct: 361 RLAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWDEDNS 420

Query: 421 EDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPP 480
           E+LVTHS+QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQP QSPP
Sbjct: 421 ENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPCQSPP 480

Query: 481 RCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 517
           RCTDSYVDTMKD+HQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 RCTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

[Close]

BLAST of Spg019971 vs. NCBI nr
Match: XP_038894440.1 (uncharacterized protein LOC120083025 [Benincasa hispida])

HSP 1 Score: 985.3 bits (2546), Expect = 2.0e-283
Identity = 465/508 (91.54%), Postives = 491/508 (96.65%), Query Frame = 0


Query: 9   RGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVDEGSEEEELDEFSVKMFFKGVSIAEIG 68
           RGS+HGPAEED+VEDFRSCCEDHEVWKETE+ V+E  E E+LDEFSVKMFFKGVSIAEIG
Sbjct: 5   RGSSHGPAEEDEVEDFRSCCEDHEVWKETEEAVEE--ESEDLDEFSVKMFFKGVSIAEIG 64

Query: 69  DPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQNDIRCIRAF 128
           DPASGLSGIGVFMEKSGK+PF+QVQKKLDFYVDELVADYLALMDGLVEAMQND+ CIRAF
Sbjct: 65  DPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQNDVCCIRAF 124

Query: 129 TDSDLLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSSTDLEQPFHLAKVAIG 188
           TDSDLLY QITNEE+PEIPLLVALKERILEHAS FEAFSLNLSSSTDLEQP HLAKVAIG
Sbjct: 125 TDSDLLYKQITNEEDPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQPLHLAKVAIG 184

Query: 189 VLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKTYVDGKLETSQVPIRCPQ 248
           VLSCPQKGDKSVENCSICC+NKPSAMMVALKCCHKFCSHCMKTYVDGK++ SQVPIRCPQ
Sbjct: 185 VLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSHCMKTYVDGKVDASQVPIRCPQ 244

Query: 249 LRCKYYIKSNEFKSFLPLTLYELLETTFAEANIQSDRIYCPFPNCSVLLDPTECLSARAS 308
           LRCKYYI SNEFKSFLPLTLYE LE T AEANI SDRIYCPFPNCSVLLDP+ECLSARAS
Sbjct: 245 LRCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDRIYCPFPNCSVLLDPSECLSARAS 304

Query: 309 SSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITLHRLAQNKRW 368
           SSSQSDNSCIECPVCQRFICVEC+VPWHSSMSCEEFQ++PL+ERDTADITLHRLAQNKRW
Sbjct: 305 SSSQSDNSCIECPVCQRFICVECQVPWHSSMSCEEFQSVPLEERDTADITLHRLAQNKRW 364

Query: 369 RRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDNSEDLVTHSI 428
           RRCQ+C RMIEL+ GCYHMTCWCGHEFCYSCG EYQDG+QTCQCAFWD+D+SE++VTHS+
Sbjct: 365 RRCQECHRMIELAHGCYHMTCWCGHEFCYSCGVEYQDGEQTCQCAFWDEDDSENVVTHSV 424

Query: 429 QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSYVD 488
           QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSYVD
Sbjct: 425 QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSYVD 484

Query: 489 TMKDLHQLPWLERFVSVISDSYYEDYVQ 517
           TMKD+HQLPWLERFVSVISDSYYEDYVQ
Sbjct: 485 TMKDIHQLPWLERFVSVISDSYYEDYVQ 510

[Close]

BLAST of Spg019971 vs. NCBI nr
Match: XP_022994789.1 (probable E3 ubiquitin-protein ligase ARI11 [Cucurbita maxima])

HSP 1 Score: 965.7 bits (2495), Expect = 1.6e-277
Identity = 470/521 (90.21%), Postives = 491/521 (94.24%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVDEGSEEEE-LDEFSVKMFF 60
           ME RE+F      GPAEEDDVEDFRSCCEDH      EDVV+EGSEEEE LDEFSVKMFF
Sbjct: 1   MEHREMF------GPAEEDDVEDFRSCCEDH------EDVVEEGSEEEEDLDEFSVKMFF 60

Query: 61  KGVSIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KG+SIAEIGDP+SGLSGIGVFME+SGKVPFIQVQKKLDFYVDELVADYLALMDGLVEA+Q
Sbjct: 61  KGISIAEIGDPSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQ 120

Query: 121 NDIRCIRAFTDSDLLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSSTDLEQP 180
           N +R IRAFTDS+ LYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSS +DLEQP
Sbjct: 121 NGVRSIRAFTDSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQP 180

Query: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKTYVDGKLET 240
           FHLAKVAIGVLSCPQKGDKS+ENCSICCENKP+AMMVALKCCHKFCSHCMK+YVDGKLET
Sbjct: 181 FHLAKVAIGVLSCPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLET 240

Query: 241 SQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLETTFAEANIQSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRCKYYI SNEFKSFLPLTLYE LE T AEANI SD+IYCP+PNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDP 300

Query: 301 TECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITL 360
           +ECLSARASSSSQSDN+CIECPVCQRFICVEC VPWHSSMSCEEFQ+IPL+ERD ADITL
Sbjct: 301 SECLSARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITL 360

Query: 361 HRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFW---- 420
           HRLA+NKRWRRCQQCRRMIEL+QGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFW    
Sbjct: 361 HRLARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDD 420

Query: 421 DDDNSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQP 480
           DDDNSEDLVTHSIQESEQWAWETFNSLPMI DAYSEQERSQLALIQRFLAGGFSLSDHQP
Sbjct: 421 DDDNSEDLVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQP 480

Query: 481 YQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 517
           YQSPPRCTDSYVDTMKDLHQLPWLERFVSVISD+YYEDYVQ
Sbjct: 481 YQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 509

[Close]

BLAST of Spg019971 vs. ExPASy Swiss-Prot
Match: D3YYI7 (Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 PE=3 SV=2)

HSP 1 Score: 95.5 bits (236), Expect = 1.9e-18
Identity = 64/220 (29.09%), Postives = 100/220 (45.45%), Query Frame = 0


Query: 203 CSICCENKPSAMMVALKCCHK-FCSHCMKTYVDGKLETSQVPIRCPQLRCKYYIKSNEFK 262
           C +C E+KP   +  L CC K  C  C+K Y+  +++  QV I+CP   C  +++  E  
Sbjct: 236 CRVCLEDKP---IKPLPCCKKAVCEECLKIYLSSQVQLGQVEIKCPVTECFEFLE--ETT 295

Query: 263 SFLPLTLYELLETTFAEANIQSDRIYCPFPNCSVLLDPTECLSARASSSSQSDNSCIECP 322
               LT  + ++  +     + D    P P C       +       S S+S    I+CP
Sbjct: 296 VVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESRYK-IQCP 355

Query: 323 VCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITLHRLAQNKRWRRCQQCRRMIELS 382
            CQ   C +C  PWH  ++C+E++      R  A    H     +  ++C +C+  I+ +
Sbjct: 356 TCQLIWCFKCHSPWHEGVNCKEYKKGDKLLRHWASEIEH---GQRNAQKCPKCKIHIQRT 415

Query: 383 QGCYHMTC-WCGHEFCYSCGAEYQDGQQTCQCAFWDDDNS 421
           +GC HMTC  C   FCY CG  Y+      Q  F+ D  S
Sbjct: 416 EGCDHMTCSQCNTNFCYRCGERYR------QLRFFGDHTS 440

[Close]

BLAST of Spg019971 vs. ExPASy Swiss-Prot
Match: Q8TC41 (Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE=2 SV=4)

HSP 1 Score: 95.1 bits (235), Expect = 2.4e-18
Identity = 64/220 (29.09%), Postives = 100/220 (45.45%), Query Frame = 0


Query: 203 CSICCENKPSAMMVALKCCHK-FCSHCMKTYVDGKLETSQVPIRCPQLRCKYYIKSNEFK 262
           C +C E+KP   +  L CC K  C  C+K Y+  +++  QV I+CP   C  +++  E  
Sbjct: 263 CRVCLEDKP---IKPLPCCKKAVCEECLKVYLSAQVQLGQVEIKCPITECFEFLE--ETT 322

Query: 263 SFLPLTLYELLETTFAEANIQSDRIYCPFPNCSVLLDPTECLSARASSSSQSDNSCIECP 322
               LT  + ++  +     + D    P P C       +       S S+S    I+CP
Sbjct: 323 VVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESKYK-IQCP 382

Query: 323 VCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITLHRLAQNKRWRRCQQCRRMIELS 382
            CQ   C +C  PWH  ++C+E++      R  A    H     +  ++C +C+  I+ +
Sbjct: 383 TCQFVWCFKCHSPWHEGVNCKEYKKGDKLLRHWASEIEH---GQRNAQKCPKCKIHIQRT 442

Query: 383 QGCYHMTC-WCGHEFCYSCGAEYQDGQQTCQCAFWDDDNS 421
           +GC HMTC  C   FCY CG  Y+      Q  F+ D  S
Sbjct: 443 EGCDHMTCSQCNTNFCYRCGERYR------QLRFFGDHTS 467

[Close]

BLAST of Spg019971 vs. ExPASy Swiss-Prot
Match: Q9SKC4 (Probable E3 ubiquitin-protein ligase ARI10 OS=Arabidopsis thaliana OX=3702 GN=ARI10 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.5e-15
Identity = 61/233 (26.18%), Postives = 93/233 (39.91%), Query Frame = 0


Query: 187 IGVLSCPQKGDKSVE---NCSICCENKPSAMMVALKCCHKFCSHCMKTYVDGKLETSQ-- 246
           +G+L  P       E    C IC E+     + ++ C H +C  C   Y+  K+E     
Sbjct: 102 VGILKEPVVDVNGTEVDIQCGICFESYTRKEIASVSCGHPYCKTCWTGYITTKIEDGPGC 161

Query: 247 VPIRCPQLRCKYYIKSNEFKSFLPLTLYELLETTFAEANIQSDR--IYCPFPNCSVLLDP 306
           + ++CP+  C   +  +           +     F  + ++  +   +CP P C      
Sbjct: 162 LRVKCPEPSCYAVVGQDMIDEVTEKKDKDKYYRYFLRSYVEDGKKMKWCPSPGC------ 221

Query: 307 TECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITL 366
            EC +     SS  D +C+    C    C  C    HS + CE         +D      
Sbjct: 222 -EC-AVEFGESSGYDVACL----CSYRFCWNCSEDAHSPVDCETVSKWIFKNQDE----- 281

Query: 367 HRLAQNKRW-----RRCQQCRRMIELSQGCYHMTC--WCGHEFCYSCGAEYQD 406
              ++NK W     + C +C+R IE S GC HMTC   CGH FC+ CG  Y D
Sbjct: 282 ---SENKNWILANSKPCPKCKRPIEKSHGCNHMTCSASCGHRFCWICGKSYSD 314

[Close]

BLAST of Spg019971 vs. ExPASy Swiss-Prot
Match: Q9SKC3 (Probable E3 ubiquitin-protein ligase ARI9 OS=Arabidopsis thaliana OX=3702 GN=ARI9 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.5e-15
Identity = 55/214 (25.70%), Postives = 88/214 (41.12%), Query Frame = 0


Query: 203 CSICCENKPSAMMVALKCCHKFCSHCMKTYVDGKLETSQ--VPIRCPQLRCKYYIKSNEF 262
           C IC E+     +  + C H +C  C   Y+  K+E     + ++CP+  C   +  +  
Sbjct: 128 CGICFESYTREEIARVSCGHPYCKTCWAGYITTKIEDGPGCLRVKCPEPSCSAAVGKDMI 187

Query: 263 KSFLPLTLYELLETTFAEANIQSDR--IYCPFPNCSVLLDPTECLSARASSSSQSDNSCI 322
           +      + E        + ++  +   +CP P C   ++         S SS  D SC+
Sbjct: 188 EDVTETKVNEKYSRYILRSYVEDGKKIKWCPSPGCGYAVE------FGGSESSSYDVSCL 247

Query: 323 ECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITLHRLAQNKRWRRCQQCRRMI 382
               C    C  C    HS + C+         +D ++     LA +K    C +C+R I
Sbjct: 248 ----CSYRFCWNCSEDAHSPVDCDTVSKWIFKNQDESENKNWMLANSK---PCPECKRPI 307

Query: 383 ELSQGCYHMTCW--CGHEFCYSCGAEYQDGQQTC 411
           E + GC HMTC   CGHEFC+ C   Y+     C
Sbjct: 308 EKNDGCNHMTCSAPCGHEFCWICLKAYRRHSGAC 328

[Close]

BLAST of Spg019971 vs. ExPASy Swiss-Prot
Match: Q6T486 (Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 GN=rbrA PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 9.5e-15
Identity = 69/284 (24.30%), Postives = 109/284 (38.38%), Query Frame = 0


Query: 202 NCSICCENKPSAMMVALKCCHKFCSHCMKTYVDGKLETSQ--VPIRCPQLRCKYYIKSNE 261
           +C IC E+ P     AL C H++C  C K Y++ K+      +   CP  +CK  +  + 
Sbjct: 138 SCLICLEDYPPTQTFALICNHRYCLPCYKNYLEIKVSEGPECIYTPCPAPKCKVIVHQDA 197

Query: 262 FKSFLPLTLYELLETTFAEANIQSDR--IYCPFPNCSVLLDPTECLSARASSSSQSDNSC 321
           FK  +   ++E       ++ +  +    +CP P C          S R     + +   
Sbjct: 198 FKQIVSPEVFERFNNFILKSYVDDNPQVKWCPAPGC--------IYSIRCDRKERKEAVN 257

Query: 322 IECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITLHRLAQNKRWRRCQQCRRM 381
            +C     F C +  +  H    C +         D ++     LA  K   +C +CR  
Sbjct: 258 CKCGFQYCFNCNDYEIGDHMPCPCSQVDKWLQKASDESENVTWMLANTK---KCPECRSP 317

Query: 382 IELSQGCYHMTC-----WCGHEFCYSC-----------GAEYQDGQQTCQCAFWDDDNSE 441
           IE + GC HMTC      CG EFC+ C           G  Y   +     A  DDD + 
Sbjct: 318 IEKNGGCMHMTCRKNAGGCGFEFCWLCRGPWTEHGSTTGGYYNCNKYDKSKAKEDDDKAH 377

Query: 442 DLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLA 466
           D  T    E+  + +  + S    M    EQ R+     Q+ L+
Sbjct: 378 DAKTE--LEAYMFYYHRYESHRNAMKIADEQRRNAHLKEQQILS 408

[Close]

BLAST of Spg019971 vs. ExPASy TrEMBL
Match: A0A1S3C9E4 (E3 ubiquitin-protein ligase arih1 OS=Cucumis melo OX=3656 GN=LOC103498311 PE=3 SV=1)

HSP 1 Score: 1003.0 bits (2592), Expect = 4.4e-289
Identity = 475/516 (92.05%), Postives = 500/516 (96.90%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVDEGSEEEELDEFSVKMFFK 60
           ME R+IFGRGS+HGPAEEDDVE+FRSCCEDHEVWKETED V+E S EE+LDEFSVKMFFK
Sbjct: 1   MEHRQIFGRGSSHGPAEEDDVEEFRSCCEDHEVWKETEDAVEEES-EEDLDEFSVKMFFK 60

Query: 61  GVSIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQN 120
           GVSIAEIGDPASGLSGIGVFMEKSGK+PF+QVQKKLDFYVDELVADYLALMDGLVEAMQN
Sbjct: 61  GVSIAEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQN 120

Query: 121 DIRCIRAFTDSDLLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSSTDLEQPF 180
           D+RCIRAFTDSDLLY QITNEEEPEIPLLVALKERILEHAS FE+FSL LSSS DLEQPF
Sbjct: 121 DVRCIRAFTDSDLLYKQITNEEEPEIPLLVALKERILEHASNFESFSLILSSSADLEQPF 180

Query: 181 HLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKTYVDGKLETS 240
           HLAKVAIGVLSCPQKGDKSVENCSICC+NKPSAMMVALKCCHKFCS C+KTYVDGK++ S
Sbjct: 181 HLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCLKTYVDGKVDAS 240

Query: 241 QVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLETTFAEANIQSDRIYCPFPNCSVLLDPT 300
           QVPIRCPQLRCKYYI SNEFKSFLPLTLYELLE T AEANI +DRIYCPFPNCSVLLDP+
Sbjct: 241 QVPIRCPQLRCKYYITSNEFKSFLPLTLYELLENTLAEANIHADRIYCPFPNCSVLLDPS 300

Query: 301 ECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITLH 360
           ECLSARASSSSQSDNSCIECPVCQRFICVEC+VPWHSS+SCEEFQNIPL+ERDTADITLH
Sbjct: 301 ECLSARASSSSQSDNSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLH 360

Query: 361 RLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDNS 420
           RLAQNKRWRRCQ+C RMIEL+QGC+HMTCWCGHEFCYSCG EYQDGQQTCQCAFWD+DNS
Sbjct: 361 RLAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWDEDNS 420

Query: 421 EDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPP 480
           E+LVTHS+QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPP
Sbjct: 421 ENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPP 480

Query: 481 RCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 517
           RCTDSYVDTMKD+HQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 RCTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

[Close]

BLAST of Spg019971 vs. ExPASy TrEMBL
Match: A0A5D3E1X1 (E3 ubiquitin-protein ligase arih1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G001170 PE=3 SV=1)

HSP 1 Score: 1003.0 bits (2592), Expect = 4.4e-289
Identity = 475/516 (92.05%), Postives = 500/516 (96.90%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVDEGSEEEELDEFSVKMFFK 60
           ME R+IFGRGS+HGPAEEDDVE+FRSCCEDHEVWKETED V+E S EE+LDEFSVKMFFK
Sbjct: 1   MEHRQIFGRGSSHGPAEEDDVEEFRSCCEDHEVWKETEDAVEEES-EEDLDEFSVKMFFK 60

Query: 61  GVSIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQN 120
           GVSIAEIGDPASGLSGIGVFMEKSGK+PF+QVQKKLDFYVDELVADYLALMDGLVEAMQN
Sbjct: 61  GVSIAEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQN 120

Query: 121 DIRCIRAFTDSDLLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSSTDLEQPF 180
           D+RCIRAFTDSDLLY QITNEEEPEIPLLVALKERILEHAS FE+FSL LSSS DLEQPF
Sbjct: 121 DVRCIRAFTDSDLLYKQITNEEEPEIPLLVALKERILEHASNFESFSLILSSSADLEQPF 180

Query: 181 HLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKTYVDGKLETS 240
           HLAKVAIGVLSCPQKGDKSVENCSICC+NKPSAMMVALKCCHKFCS C+KTYVDGK++ S
Sbjct: 181 HLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCLKTYVDGKVDAS 240

Query: 241 QVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLETTFAEANIQSDRIYCPFPNCSVLLDPT 300
           QVPIRCPQLRCKYYI SNEFKSFLPLTLYELLE T AEANI +DRIYCPFPNCSVLLDP+
Sbjct: 241 QVPIRCPQLRCKYYITSNEFKSFLPLTLYELLENTLAEANIHADRIYCPFPNCSVLLDPS 300

Query: 301 ECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITLH 360
           ECLSARASSSSQSDNSCIECPVCQRFICVEC+VPWHSS+SCEEFQNIPL+ERDTADITLH
Sbjct: 301 ECLSARASSSSQSDNSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLH 360

Query: 361 RLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDNS 420
           RLAQNKRWRRCQ+C RMIEL+QGC+HMTCWCGHEFCYSCG EYQDGQQTCQCAFWD+DNS
Sbjct: 361 RLAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWDEDNS 420

Query: 421 EDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPP 480
           E+LVTHS+QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPP
Sbjct: 421 ENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPP 480

Query: 481 RCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 517
           RCTDSYVDTMKD+HQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 RCTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

[Close]

BLAST of Spg019971 vs. ExPASy TrEMBL
Match: A0A6J1CLL9 (uncharacterized protein LOC111012584 OS=Momordica charantia OX=3673 GN=LOC111012584 PE=3 SV=1)

HSP 1 Score: 995.3 bits (2572), Expect = 9.2e-287
Identity = 479/517 (92.65%), Postives = 495/517 (95.74%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVDEGSEEEE-LDEFSVKMFF 60
           ME REIFGRGS+HGPAEEDDVEDFRSCCEDHEVWKE ED+V+EGSEEEE LDEFSVKMFF
Sbjct: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKEAEDLVEEGSEEEEHLDEFSVKMFF 60

Query: 61  KGVSIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KG+SIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGISIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIRCIRAFTDSDLLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSSTDLEQP 180
           N+IRCIRAFTDSDLLYNQITNEEE EIPLL+ALKERILEHASTFEAFSLNLSSSTDLEQP
Sbjct: 121 NEIRCIRAFTDSDLLYNQITNEEESEIPLLIALKERILEHASTFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKTYVDGKLET 240
           FHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMV LKCCHKFCSHCMKTYVDGK+++
Sbjct: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVGLKCCHKFCSHCMKTYVDGKVQS 240

Query: 241 SQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLETTFAEANIQSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRCKYYI  NEF+SFLPLTLYE LE T AEANI SDRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITINEFRSFLPLTLYESLENTLAEANIHSDRIYCPFPNCSVLLDP 300

Query: 301 TECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITL 360
           +ECLS RASSSSQSDNSCIECPVCQRFICV C VPWHSSMSCEEFQNI     DT DITL
Sbjct: 301 SECLSGRASSSSQSDNSCIECPVCQRFICVYCGVPWHSSMSCEEFQNI-----DTEDITL 360

Query: 361 HRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDN 420
           HRLAQNKRW+RCQQCRRMIEL+QGCYHMTCWCGHEFCYSCGAEYQD QQTCQCA WD++N
Sbjct: 361 HRLAQNKRWKRCQQCRRMIELTQGCYHMTCWCGHEFCYSCGAEYQDSQQTCQCALWDEEN 420

Query: 421 SEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQP+QSP
Sbjct: 421 SEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPHQSP 480

Query: 481 PRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 517
           PRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 PRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 512

[Close]

BLAST of Spg019971 vs. ExPASy TrEMBL
Match: A0A0A0M0W5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G706610 PE=3 SV=1)

HSP 1 Score: 988.8 bits (2555), Expect = 8.6e-285
Identity = 469/516 (90.89%), Postives = 494/516 (95.74%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVDEGSEEEELDEFSVKMFFK 60
           ME REIFGRGS+HGPAEEDDVEDFRSCCEDHEVWKETED V+E S EE+LDEFSVKMFFK
Sbjct: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEES-EEDLDEFSVKMFFK 60

Query: 61  GVSIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQN 120
           GVSIAEIGD ASGLSGIGV MEKSGK+PF+QVQKKLDFYVDELVADYLALMDGLVEAMQN
Sbjct: 61  GVSIAEIGDSASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQN 120

Query: 121 DIRCIRAFTDSDLLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSSTDLEQPF 180
           D+RCIRAFT SDLLY QITN EEPEIPLLVALKERILEHAS FE+FSL+LSS  DL+QPF
Sbjct: 121 DVRCIRAFTSSDLLYKQITNHEEPEIPLLVALKERILEHASNFESFSLSLSSGADLQQPF 180

Query: 181 HLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKTYVDGKLETS 240
           HLAKVAIGVLSCPQKGDKSVENCSICC+NKPSAMMVALKCCHKFCS CMKTYVDGK++ S
Sbjct: 181 HLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDAS 240

Query: 241 QVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLETTFAEANIQSDRIYCPFPNCSVLLDPT 300
           QVPIRCPQLRCKYYI SNEFKSFLPLTLYE LE T AEANI +DRIYCPFPNCSVLLDP+
Sbjct: 241 QVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDPS 300

Query: 301 ECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITLH 360
           ECLSARASSSSQS+NSCIECPVCQRFICVEC+VPWHSS+SCEEFQNIPL+ERDTADITLH
Sbjct: 301 ECLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLH 360

Query: 361 RLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDNS 420
           RLAQNKRWRRCQ+C RMIEL+QGC+HMTCWCGHEFCYSCG EYQDGQQTCQCAFWD+DNS
Sbjct: 361 RLAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWDEDNS 420

Query: 421 EDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPP 480
           E+LVTHS+QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQP QSPP
Sbjct: 421 ENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPCQSPP 480

Query: 481 RCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 517
           RCTDSYVDTMKD+HQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 RCTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

[Close]

BLAST of Spg019971 vs. ExPASy TrEMBL
Match: A0A6J1K3V2 (probable E3 ubiquitin-protein ligase ARI11 OS=Cucurbita maxima OX=3661 GN=LOC111490412 PE=3 SV=1)

HSP 1 Score: 965.7 bits (2495), Expect = 7.8e-278
Identity = 470/521 (90.21%), Postives = 491/521 (94.24%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVDEGSEEEE-LDEFSVKMFF 60
           ME RE+F      GPAEEDDVEDFRSCCEDH      EDVV+EGSEEEE LDEFSVKMFF
Sbjct: 1   MEHREMF------GPAEEDDVEDFRSCCEDH------EDVVEEGSEEEEDLDEFSVKMFF 60

Query: 61  KGVSIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KG+SIAEIGDP+SGLSGIGVFME+SGKVPFIQVQKKLDFYVDELVADYLALMDGLVEA+Q
Sbjct: 61  KGISIAEIGDPSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQ 120

Query: 121 NDIRCIRAFTDSDLLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSSTDLEQP 180
           N +R IRAFTDS+ LYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSS +DLEQP
Sbjct: 121 NGVRSIRAFTDSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQP 180

Query: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKTYVDGKLET 240
           FHLAKVAIGVLSCPQKGDKS+ENCSICCENKP+AMMVALKCCHKFCSHCMK+YVDGKLET
Sbjct: 181 FHLAKVAIGVLSCPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLET 240

Query: 241 SQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLETTFAEANIQSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRCKYYI SNEFKSFLPLTLYE LE T AEANI SD+IYCP+PNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDP 300

Query: 301 TECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITL 360
           +ECLSARASSSSQSDN+CIECPVCQRFICVEC VPWHSSMSCEEFQ+IPL+ERD ADITL
Sbjct: 301 SECLSARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITL 360

Query: 361 HRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFW---- 420
           HRLA+NKRWRRCQQCRRMIEL+QGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFW    
Sbjct: 361 HRLARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDD 420

Query: 421 DDDNSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQP 480
           DDDNSEDLVTHSIQESEQWAWETFNSLPMI DAYSEQERSQLALIQRFLAGGFSLSDHQP
Sbjct: 421 DDDNSEDLVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQP 480

Query: 481 YQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 517
           YQSPPRCTDSYVDTMKDLHQLPWLERFVSVISD+YYEDYVQ
Sbjct: 481 YQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 509

[Close]

BLAST of Spg019971 vs. TAIR 10
Match: AT4G19670.1 (RING/U-box superfamily protein )

HSP 1 Score: 664.8 bits (1714), Expect = 5.5e-191
Identity = 335/514 (65.18%), Postives = 407/514 (79.18%), Query Frame = 0


Query: 20  DVEDFRSCCEDHEVWKETED----VVDEGSEEEELDEFSVKMFFKGVSIAEIGDPASGLS 79
           D E+FRSCC D E+W +  D    VV+E  +++  DEFSVKM FKGVSI E GD  SG S
Sbjct: 20  DEEEFRSCCGDEELWLKGSDDIAKVVEEEKKDDLDDEFSVKMVFKGVSIFERGDLGSGYS 79

Query: 80  GIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQNDIRCIRAFTDSDLLY 139
           GIGV +E+SG +  IQVQKKLDFYV+E VA+YLALMDGL  A+QN++  + A TDS+LLY
Sbjct: 80  GIGVVLERSGDLELIQVQKKLDFYVEESVANYLALMDGLEVALQNNLSSVVAVTDSELLY 139

Query: 140 NQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSSTDLEQPFHLAKVAIGVLSCPQK 199
           NQIT EE+ EIPLLVAL+ER+LE  S    F L L+   DL++   LA+VA+G++S    
Sbjct: 140 NQITREEQLEIPLLVALRERVLEKTSNLNGFVLKLAPFCDLDEALSLAQVAVGIVSSNLD 199

Query: 200 GDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKTYVDGKLETSQVPIRCPQLRCKYYI 259
           GDK +ENCSICCE++ S MM++LKC HKFCSHCMKTYV+GK++TS+VPIRCPQ++CK+Y+
Sbjct: 200 GDKPIENCSICCEDRQSEMMLSLKCTHKFCSHCMKTYVEGKVKTSEVPIRCPQVQCKHYL 259

Query: 260 KSNEFKSFLPLTLYELLETTFAEANI---QSDRIYCPFPNCSVLLDPTECLS---ARASS 319
            + E KSFLP+T ++    +F EAN+    + +IYCP+PNCS LLDP ECLS   A +SS
Sbjct: 260 SAAECKSFLPVTTFK----SFEEANVCSKNNGKIYCPYPNCSFLLDPQECLSSGRASSSS 319

Query: 320 SSQSDNS-CIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITLHRLAQNKRW 379
           S+QS+NS C+ECPVC+RF+CV+C VPWH+SMSCEEFQ +P+DER   DITLHRLA+ KRW
Sbjct: 320 STQSENSCCVECPVCERFVCVDCGVPWHASMSCEEFQILPVDERYPDDITLHRLARYKRW 379

Query: 380 RRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDNSED---LVT 439
           RRCQQCR MIEL+QGC HMTC CGHEFCYSCGAEY++GQQTC CAFWDDD  E    +  
Sbjct: 380 RRCQQCRIMIELAQGCNHMTCRCGHEFCYSCGAEYREGQQTCTCAFWDDDEEEQENAVEE 439

Query: 440 HSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLA-GGFSLSDHQ-PYQS-PPRC 499
           ++IQE EQW W+TFNS+P +MDAYSEQERSQLALIQRFLA GGFSLSDH   YQS PP C
Sbjct: 440 NTIQEVEQWPWDTFNSIPTVMDAYSEQERSQLALIQRFLAGGGFSLSDHHTTYQSPPPPC 499

Query: 500 TD-SYVD-TMKDLHQLPWLERFVSVISDSYYEDY 515
           T+ SYV+  MKDLHQLPWLERFVSVISD YYE+Y
Sbjct: 500 TESSYVEAAMKDLHQLPWLERFVSVISDDYYEEY 529

[Close]

BLAST of Spg019971 vs. TAIR 10
Match: AT4G19670.2 (RING/U-box superfamily protein )

HSP 1 Score: 664.8 bits (1714), Expect = 5.5e-191
Identity = 335/514 (65.18%), Postives = 407/514 (79.18%), Query Frame = 0


Query: 20  DVEDFRSCCEDHEVWKETED----VVDEGSEEEELDEFSVKMFFKGVSIAEIGDPASGLS 79
           D E+FRSCC D E+W +  D    VV+E  +++  DEFSVKM FKGVSI E GD  SG S
Sbjct: 20  DEEEFRSCCGDEELWLKGSDDIAKVVEEEKKDDLDDEFSVKMVFKGVSIFERGDLGSGYS 79

Query: 80  GIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQNDIRCIRAFTDSDLLY 139
           GIGV +E+SG +  IQVQKKLDFYV+E VA+YLALMDGL  A+QN++  + A TDS+LLY
Sbjct: 80  GIGVVLERSGDLELIQVQKKLDFYVEESVANYLALMDGLEVALQNNLSSVVAVTDSELLY 139

Query: 140 NQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSSTDLEQPFHLAKVAIGVLSCPQK 199
           NQIT EE+ EIPLLVAL+ER+LE  S    F L L+   DL++   LA+VA+G++S    
Sbjct: 140 NQITREEQLEIPLLVALRERVLEKTSNLNGFVLKLAPFCDLDEALSLAQVAVGIVSSNLD 199

Query: 200 GDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKTYVDGKLETSQVPIRCPQLRCKYYI 259
           GDK +ENCSICCE++ S MM++LKC HKFCSHCMKTYV+GK++TS+VPIRCPQ++CK+Y+
Sbjct: 200 GDKPIENCSICCEDRQSEMMLSLKCTHKFCSHCMKTYVEGKVKTSEVPIRCPQVQCKHYL 259

Query: 260 KSNEFKSFLPLTLYELLETTFAEANI---QSDRIYCPFPNCSVLLDPTECLS---ARASS 319
            + E KSFLP+T ++    +F EAN+    + +IYCP+PNCS LLDP ECLS   A +SS
Sbjct: 260 SAAECKSFLPVTTFK----SFEEANVCSKNNGKIYCPYPNCSFLLDPQECLSSGRASSSS 319

Query: 320 SSQSDNS-CIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTADITLHRLAQNKRW 379
           S+QS+NS C+ECPVC+RF+CV+C VPWH+SMSCEEFQ +P+DER   DITLHRLA+ KRW
Sbjct: 320 STQSENSCCVECPVCERFVCVDCGVPWHASMSCEEFQILPVDERYPDDITLHRLARYKRW 379

Query: 380 RRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDNSED---LVT 439
           RRCQQCR MIEL+QGC HMTC CGHEFCYSCGAEY++GQQTC CAFWDDD  E    +  
Sbjct: 380 RRCQQCRIMIELAQGCNHMTCRCGHEFCYSCGAEYREGQQTCTCAFWDDDEEEQENAVEE 439

Query: 440 HSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLA-GGFSLSDHQ-PYQS-PPRC 499
           ++IQE EQW W+TFNS+P +MDAYSEQERSQLALIQRFLA GGFSLSDH   YQS PP C
Sbjct: 440 NTIQEVEQWPWDTFNSIPTVMDAYSEQERSQLALIQRFLAGGGFSLSDHHTTYQSPPPPC 499

Query: 500 TD-SYVD-TMKDLHQLPWLERFVSVISDSYYEDY 515
           T+ SYV+  MKDLHQLPWLERFVSVISD YYE+Y
Sbjct: 500 TESSYVEAAMKDLHQLPWLERFVSVISDDYYEEY 529

[Close]

BLAST of Spg019971 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 187.6 bits (475), Expect = 2.6e-47
Identity = 120/379 (31.66%), Postives = 200/379 (52.77%), Query Frame = 0


Query: 56  KMFFKGVSIAE--IGDPASGLSGIGV-FMEKSGKVPFIQVQKKLDFYVDELVADYLALMD 115
           +++FKG+   E   G     +SG GV   ++   + F      +D  +    A+  AL+ 
Sbjct: 146 RLYFKGLVSDENGKGKMTDVVSGFGVAICDQRDNLLFEMKGPLIDNGMSRQGAELKALIR 205

Query: 116 GLVEAMQNDIRCIRAFTDSDLLYNQITNE---EEPEIPLLVALKERILEHASTFEAFSLN 175
           GL EA++  I+ I  F DS  ++  +T +   ++ +I LL+   + I++H S+++     
Sbjct: 206 GLTEALKLGIKHIVFFCDSYPIFQYVTGKWMAKQKKISLLLDDLQSIMQHFSSYQHV--- 265

Query: 176 LSSSTDLEQPFHLAKVAIGVLSCPQKGDKSV-----ENCSICCENKPSAMMVAL-KCCHK 235
           L +  D++  + LA+ +I  +  P +  +       E C+IC  +  +  M ++ KC H+
Sbjct: 266 LVARNDVKFAYKLARESILSMVTPHEDPRQAKAVLKEECAICFNDIVAEGMFSVDKCRHR 325

Query: 236 FCSHCMKTYVDGKLETSQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLETTFAEANIQ- 295
           FC  C+K +V+ KL     P +CP   CK  +  +     L   L +L +    E  I  
Sbjct: 326 FCFQCVKQHVEVKLLHGMAP-KCPHDGCKSELVIDACGKLLTPKLSKLWQQRLQENAIPV 385

Query: 296 SDRIYCPFPNCSVLLDPTECL-SARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSC 355
           ++R+YCP+P CS L+  T+   SA++  S    +    C  C+   CV+C+VPWH ++SC
Sbjct: 386 TERVYCPYPRCSALMSKTKISESAKSLLSLYPKSGVRRCVECRGLFCVDCKVPWHGNLSC 445

Query: 356 EEFQNIPLDERDTADITLHRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGA 415
            E++ +   E    D+ L  LA NK WR+C +C+ MIELSQGC H+TC CGHEFCY+CG 
Sbjct: 446 TEYKKLH-PEPPADDVKLKSLANNKMWRQCGKCQHMIELSQGCNHITCRCGHEFCYNCGG 505

Query: 416 EYQDGQQTC--QCAFWDDD 419
            +     TC  +C  W+++
Sbjct: 506 GWNKIMGTCLNRCPTWNEE 519

[Close]

BLAST of Spg019971 vs. TAIR 10
Match: AT2G25360.1 (RING/U-box superfamily protein )

HSP 1 Score: 181.8 bits (460), Expect = 1.4e-45
Identity = 112/308 (36.36%), Postives = 167/308 (54.22%), Query Frame = 0


Query: 108 LALMDGLVEAMQNDIRCIRAFTDSDLLYNQIT--NEEEPEIPLLVALKERILEHASTFEA 167
           +AL+ GL E+    IR +  + D  LLY  I    + + ++  LV   + +LE  +  +A
Sbjct: 1   MALIRGLRESFDLGIRQVVVYCDDQLLYQYIVGRGKIKKKVVHLVDEFQLLLEEMTYTDA 60

Query: 168 FSLNLSSSTDLEQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMM--VALKCCHK 227
              +L +  D+   F LA+ AI       + D   E CSIC E      M     KC H+
Sbjct: 61  ---DLIALNDVNFAFKLAREAI-----VSRDDVKAEICSICFEETEGERMFFTTEKCVHR 120

Query: 228 FCSHCMKTYVDGKLETSQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLETTFAEANI-Q 287
            C  C+K YV+ KL +  VP  C    CK+ +        L L L E+ +    E +I  
Sbjct: 121 HCFPCVKQYVEVKLLSGTVP-TCLDDGCKFKLTLESCSKVLTLELIEMWKQKMKEDSIPA 180

Query: 288 SDRIYCPFPNCSVLLDPTECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCE 347
           ++RIYCP+PNCS+L+  TE  S    S   +D SC++C  C  F C++C+VP HS +SC 
Sbjct: 181 AERIYCPYPNCSMLMSKTELSS---ESDLSNDRSCVKC--CGLF-CIDCKVPSHSDLSCA 240

Query: 348 EFQNIPLDERDTADITLHRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAE 407
           E++ +  D     ++ L  LA++K+WR+C+ CR MIELS  C HMTC CG++FCY C  E
Sbjct: 241 EYKKLHHDPL-VDELKLKSLAKDKKWRQCKMCRHMIELSHACNHMTCRCGYQFCYQCEVE 292

Query: 408 YQDGQQTC 411
           +++ Q+TC
Sbjct: 301 WKNDQKTC 292

[Close]

BLAST of Spg019971 vs. TAIR 10
Match: AT2G25370.1 (RING/U-box superfamily protein )

HSP 1 Score: 174.1 bits (440), Expect = 2.9e-43
Identity = 120/364 (32.97%), Postives = 187/364 (51.37%), Query Frame = 0


Query: 50  LDEFSVKMFFKG-VSIAEIGDPASGL----SGIGVFMEKSGKVPFIQVQKKLDFYVDELV 109
           +D    ++ FKG V+   + D          G+ +  EK   +  I+ +   D  +    
Sbjct: 149 MDNVVYRLCFKGLVNYEAVADDVEKAVKAGFGVAICDEKDNLLHEIK-ESLRDIEISRRG 208

Query: 110 ADYLALMDGLVEAMQNDIRCIRAFTDSDLLYNQI--TNEEEPEIPLLVALKERILEHAST 169
            + +AL+ GL E+    +R +  + D D +Y  I    + + +I  LV   + ILE  + 
Sbjct: 209 VEIMALVRGLSESFDLGMRNVVIYCDDDWIYQSIIGRGKSKKKIDHLVEEVQGILEKMAC 268

Query: 170 FEAFSLNLSSSTDLEQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKP-SAMMVALKCC 229
            +A    L +  D++  F LA+ AIG  S     ++  E C I  E      M V  KC 
Sbjct: 269 IDAV---LVARNDVKFAFRLAREAIGRNSVDVNAEQG-ETCGIFFEETDVEHMFVTEKCL 328

Query: 230 HKFCSHCMKTYVDGKLETSQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLETTFAEANI 289
           H+ C  C+K +V  KL +   P  C +  CK+ +        L L L E+ +    E +I
Sbjct: 329 HRHCFPCVKQHVKVKLRSGTEP-TCLEYGCKFKLTLERCSKVLTLKLIEMWKQKMKEDSI 388

Query: 290 -QSDRIYCPFPNCSVLLDPTECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMS 349
             ++RIYCP+PNCS+L+  TE LS+ A  S+    +C++C  C  F C++C+VP H+ +S
Sbjct: 389 PAAERIYCPYPNCSMLMSKTE-LSSEADLSNV--RTCVKC--CGLF-CIDCKVPSHTDLS 448

Query: 350 CEEFQNIPLDERDTADITLHRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCG 405
            ++++ +  D     D+ L  LA +K WR+C +CR MIELS GC HMTC CG+EFCY CG
Sbjct: 449 YDDYKKLHPDPL-VDDLKLKSLANDKMWRQCVKCRHMIELSHGCNHMTCRCGYEFCYECG 499

The following BLAST results are available for this feature:

BLAST of Spg019971 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008459101.1	9.1e-289	92.05	PREDICTED: E3 ubiquitin-protein ligase arih1 [Cucumis melo] >XP_008459102.1 PRED...	[more]
XP_022142466.1	1.9e-286	92.65	uncharacterized protein LOC111012584 [Momordica charantia] >XP_022142467.1 uncha...	[more]
XP_004145453.1	1.8e-284	90.89	E3 ubiquitin-protein ligase arih1 [Cucumis sativus] >XP_011660321.1 E3 ubiquitin...	[more]
XP_038894440.1	2.0e-283	91.54	uncharacterized protein LOC120083025 [Benincasa hispida]	[more]
XP_022994789.1	1.6e-277	90.21	probable E3 ubiquitin-protein ligase ARI11 [Cucurbita maxima]	[more]

BLAST of Spg019971 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
D3YYI7	1.9e-18	29.09	Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 P...	[more]
Q8TC41	2.4e-18	29.09	Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE...	[more]
Q9SKC4	1.5e-15	26.18	Probable E3 ubiquitin-protein ligase ARI10 OS=Arabidopsis thaliana OX=3702 GN=AR...	[more]
Q9SKC3	1.5e-15	25.70	Probable E3 ubiquitin-protein ligase ARI9 OS=Arabidopsis thaliana OX=3702 GN=ARI...	[more]
Q6T486	9.5e-15	24.30	Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 G...	[more]

BLAST of Spg019971 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C9E4	4.4e-289	92.05	E3 ubiquitin-protein ligase arih1 OS=Cucumis melo OX=3656 GN=LOC103498311 PE=3 S...	[more]
A0A5D3E1X1	4.4e-289	92.05	E3 ubiquitin-protein ligase arih1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A6J1CLL9	9.2e-287	92.65	uncharacterized protein LOC111012584 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A0A0M0W5	8.6e-285	90.89	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G706610 PE=3 SV=1	[more]
A0A6J1K3V2	7.8e-278	90.21	probable E3 ubiquitin-protein ligase ARI11 OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]

BLAST of Spg019971 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19670.1	5.5e-191	65.18	RING/U-box superfamily protein	[more]
AT4G19670.2	5.5e-191	65.18	RING/U-box superfamily protein	[more]
AT5G60250.1	2.6e-47	31.66	zinc finger (C3HC4-type RING finger) family protein	[more]
AT2G25360.1	1.4e-45	36.36	RING/U-box superfamily protein	[more]
AT2G25370.1	2.9e-43	32.97	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002867	IBR domain	SMART	SM00647	ibrneu5	coord: 351..410 e-value: 0.066 score: 14.9 coord: 270..341 e-value: 5.4E-10 score: 49.2
IPR002867	IBR domain	PFAM	PF01485	IBR	coord: 281..341 e-value: 2.9E-9 score: 37.0 coord: 362..410 e-value: 2.6E-6 score: 27.5
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 203..249 e-value: 0.0039 score: 26.4 coord: 371..412 e-value: 11.0 score: 2.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 203..250 score: 10.792683
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 73..169 e-value: 1.6E-8 score: 34.4
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 197..282 e-value: 1.8E-13 score: 52.2
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 55..188 e-value: 6.6E-7 score: 31.3
None	No IPR available	GENE3D	1.20.120.1750		coord: 338..465 e-value: 3.0E-15 score: 58.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR11685:SF353	RBR-TYPE E3 UBIQUITIN TRANSFERASE	coord: 18..515
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 354..418
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 198..279
IPR031127	E3 ubiquitin ligase RBR family	PANTHER	PTHR11685	RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING	coord: 18..515
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 220..229
IPR044066	TRIAD supradomain	PROSITE	PS51873	TRIAD	coord: 199..416 score: 29.094425
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 72..166

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg019971.1	Spg019971.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process	GO:0000209	protein polyubiquitination
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0004386	helicase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0004842	ubiquitin-protein transferase activity