Spg019810 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg019810
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRNase H domain-containing protein
Locationscaffold5: 31785645 .. 31786064 (+)
RNA-Seq ExpressionSpg019810
SyntenySpg019810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGAGATGAGGGCGATTGCAGAAGGGTTGAGTTGCTTACCGTCAATCAGAGCCTCCCGCCCGGATTTGCATGTCCCTCCCATTGTTGTTGAATCAGACGCTCTTAGCGTGATTAAGCTACTGAACAAGAAGGATGAAAACCTGTCTGAAATCACCTTCTTGGTTGATGAGATCGAGCAGCTGAGGAGTTCTTTTGGGGAATTTTCTTTCTCCTTTTGCCCGCGTTCGAGAAACGAGCATGCGCATTCTTTGGCGCGCAGTTTTTTGGGTGTTGGGGCTGGTTCTATTTTACCTTTTCTGGGGCCCTCTTCCATTTCGGAAGATTGTATTGATGCACAATTTATTTTTTTGGGTCGGGTCCTACTCGACTTTCTCCTTATTCTAAGGGAGGCTGATGTTTCCTGTTTGCTTGGTTAA

mRNA sequence

ATGTTGGAGATGAGGGCGATTGCAGAAGGGTTGAGTTGCTTACCGTCAATCAGAGCCTCCCGCCCGGATTTGCATGTCCCTCCCATTGTTGTTGAATCAGACGCTCTTAGCGTGATTAAGCTACTGAACAAGAAGGATGAAAACCTGTCTGAAATCACCTTCTTGGTTGATGAGATCGAGCAGCTGAGGAGTTCTTTTGGGGAATTTTCTTTCTCCTTTTGCCCGCGTTCGAGAAACGAGCATGCGCATTCTTTGGCGCGCAGTTTTTTGGGTGTTGGGGCTGGTTCTATTTTACCTTTTCTGGGGCCCTCTTCCATTTCGGAAGATTGTATTGATGCACAATTTATTTTTTTGGGTCGGGTCCTACTCGACTTTCTCCTTATTCTAAGGGAGGCTGATGTTTCCTGTTTGCTTGGTTAA

Coding sequence (CDS)

ATGTTGGAGATGAGGGCGATTGCAGAAGGGTTGAGTTGCTTACCGTCAATCAGAGCCTCCCGCCCGGATTTGCATGTCCCTCCCATTGTTGTTGAATCAGACGCTCTTAGCGTGATTAAGCTACTGAACAAGAAGGATGAAAACCTGTCTGAAATCACCTTCTTGGTTGATGAGATCGAGCAGCTGAGGAGTTCTTTTGGGGAATTTTCTTTCTCCTTTTGCCCGCGTTCGAGAAACGAGCATGCGCATTCTTTGGCGCGCAGTTTTTTGGGTGTTGGGGCTGGTTCTATTTTACCTTTTCTGGGGCCCTCTTCCATTTCGGAAGATTGTATTGATGCACAATTTATTTTTTTGGGTCGGGTCCTACTCGACTTTCTCCTTATTCTAAGGGAGGCTGATGTTTCCTGTTTGCTTGGTTAA

Protein sequence

MLEMRAIAEGLSCLPSIRASRPDLHVPPIVVESDALSVIKLLNKKDENLSEITFLVDEIEQLRSSFGEFSFSFCPRSRNEHAHSLARSFLGVGAGSILPFLGPSSISEDCIDAQFIFLGRVLLDFLLILREADVSCLLG
Homology
BLAST of Spg019810 vs. NCBI nr
Match: KAA3477308.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 57.8 bits (138), Expect = 8.8e-05
Identity = 32/91 (35.16%), Postives = 56/91 (61.54%), Query Frame = 0

Query: 2    LEMRAIAEGLSCLPSIRASRPDLHV--PPIVVESDALSVIKLLNKKDENLSEITFLVDEI 61
            ++   +AE ++C    RAS+  L++    +++E D+LS+IK  NK+D + S+++  + +I
Sbjct: 1003 VDSAVVAEAIACR---RASQIGLNINQEVVIIEGDSLSIIKKCNKRDFDKSQVSSYIHDI 1062

Query: 62   EQLRSSFGEFSFSFCPRSRNEHAHSLARSFL 91
              L++ F + SF F PRS N  AH+LA   L
Sbjct: 1063 HILKNRFKKVSFEFVPRSANNLAHTLATETL 1090

BLAST of Spg019810 vs. NCBI nr
Match: KAA3466751.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 57.0 bits (136), Expect = 1.5e-04
Identity = 32/86 (37.21%), Postives = 55/86 (63.95%), Query Frame = 0

Query: 7   IAEGLSCLPSIRASRPDLHV--PPIVVESDALSVIKLLNKKDENLSEITFLVDEIEQLRS 66
           ++E ++C    RAS+  L++    I++E D+LS+IK  NK+D + S+++  + +I+ L++
Sbjct: 361 VSEAIACR---RASQIGLNINQEDIIIEGDSLSIIKKCNKRDFDKSQVSSYIHDIQILKN 420

Query: 67  SFGEFSFSFCPRSRNEHAHSLARSFL 91
            F + SF F PRS N  AH+LA   L
Sbjct: 421 RFKKVSFVFVPRSANNLAHTLATETL 443

BLAST of Spg019810 vs. NCBI nr
Match: XP_015383853.1 (uncharacterized protein LOC107176237 [Citrus sinensis])

HSP 1 Score: 56.2 bits (134), Expect = 2.6e-04
Identity = 27/59 (45.76%), Postives = 39/59 (66.10%), Query Frame = 0

Query: 29  IVVESDALSVIKLLNKKDENLSEITFLVDEIEQLRSSFGEFSFSFCPRSRNEHAHSLAR 88
           I+VESD   V+KL+N K+ + +E+ +++ EI+ L   F  FS    PRS N HAHSLA+
Sbjct: 406 IMVESDCQEVVKLINNKEGSRTEVMWVISEIQSLSKDFQNFSCYHTPRSCNTHAHSLAK 464

BLAST of Spg019810 vs. NCBI nr
Match: MBA0841494.1 (hypothetical protein [Gossypium armourianum])

HSP 1 Score: 55.5 bits (132), Expect = 4.4e-04
Identity = 33/92 (35.87%), Postives = 52/92 (56.52%), Query Frame = 0

Query: 7   IAEGLSCLPSIRASRPDLHVPPIVVESDALSVIKLLNKKDENLSEITFLVDEIEQLRSSF 66
           IAE ++C  +I+     +    I++ESD+LS+I+   KK+ + S ++  +++I QLR   
Sbjct: 147 IAEAVACRKAIQTG-IGMKWASIIIESDSLSIIRKCKKKNPDESWVSAYINDIHQLRQKL 206

Query: 67  GEFSFSFCPRSRNEHAHSLARSFLGVGAGSIL 99
            E SF F PRS N  AH +A+  L    G  L
Sbjct: 207 KECSFEFVPRSANNLAHIIAKETLKRNEGVYL 237

BLAST of Spg019810 vs. NCBI nr
Match: XP_015384866.1 (uncharacterized protein LOC107176592 [Citrus sinensis] >XP_024953719.1 uncharacterized protein LOC107176592 [Citrus sinensis])

HSP 1 Score: 54.7 bits (130), Expect = 7.5e-04
Identity = 25/59 (42.37%), Postives = 39/59 (66.10%), Query Frame = 0

Query: 29  IVVESDALSVIKLLNKKDENLSEITFLVDEIEQLRSSFGEFSFSFCPRSRNEHAHSLAR 88
           ++VE+D   V KL+N ++ + +EI +++ EI+  R  F   SF + PRS N HAHSLA+
Sbjct: 112 LMVETDCQDVAKLINNQEGSKTEIVWVISEIQDQRKDFQHISFHYTPRSCNAHAHSLAK 170

BLAST of Spg019810 vs. ExPASy TrEMBL
Match: A0A5B6W814 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_011206 PE=4 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 4.3e-05
Identity = 32/91 (35.16%), Postives = 56/91 (61.54%), Query Frame = 0

Query: 2    LEMRAIAEGLSCLPSIRASRPDLHV--PPIVVESDALSVIKLLNKKDENLSEITFLVDEI 61
            ++   +AE ++C    RAS+  L++    +++E D+LS+IK  NK+D + S+++  + +I
Sbjct: 1003 VDSAVVAEAIACR---RASQIGLNINQEVVIIEGDSLSIIKKCNKRDFDKSQVSSYIHDI 1062

Query: 62   EQLRSSFGEFSFSFCPRSRNEHAHSLARSFL 91
              L++ F + SF F PRS N  AH+LA   L
Sbjct: 1063 HILKNRFKKVSFEFVPRSANNLAHTLATETL 1090

BLAST of Spg019810 vs. ExPASy TrEMBL
Match: A0A5B6VCL1 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_001820 PE=4 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 7.3e-05
Identity = 32/86 (37.21%), Postives = 55/86 (63.95%), Query Frame = 0

Query: 7   IAEGLSCLPSIRASRPDLHV--PPIVVESDALSVIKLLNKKDENLSEITFLVDEIEQLRS 66
           ++E ++C    RAS+  L++    I++E D+LS+IK  NK+D + S+++  + +I+ L++
Sbjct: 361 VSEAIACR---RASQIGLNINQEDIIIEGDSLSIIKKCNKRDFDKSQVSSYIHDIQILKN 420

Query: 67  SFGEFSFSFCPRSRNEHAHSLARSFL 91
            F + SF F PRS N  AH+LA   L
Sbjct: 421 RFKKVSFVFVPRSANNLAHTLATETL 443

BLAST of Spg019810 vs. ExPASy TrEMBL
Match: A0A7J9K4Z7 (RNase H domain-containing protein (Fragment) OS=Gossypium armourianum OX=34283 GN=Goarm_003982 PE=4 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 2.1e-04
Identity = 33/92 (35.87%), Postives = 52/92 (56.52%), Query Frame = 0

Query: 7   IAEGLSCLPSIRASRPDLHVPPIVVESDALSVIKLLNKKDENLSEITFLVDEIEQLRSSF 66
           IAE ++C  +I+     +    I++ESD+LS+I+   KK+ + S ++  +++I QLR   
Sbjct: 147 IAEAVACRKAIQTG-IGMKWASIIIESDSLSIIRKCKKKNPDESWVSAYINDIHQLRQKL 206

Query: 67  GEFSFSFCPRSRNEHAHSLARSFLGVGAGSIL 99
            E SF F PRS N  AH +A+  L    G  L
Sbjct: 207 KECSFEFVPRSANNLAHIIAKETLKRNEGVYL 237

BLAST of Spg019810 vs. ExPASy TrEMBL
Match: A0A5C7ITL2 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_001058 PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 4.7e-04
Identity = 33/85 (38.82%), Postives = 51/85 (60.00%), Query Frame = 0

Query: 7   IAEGLSCLPSIRASRPDLHVPPIVVESDALSVIKLLNKKDENLSEITFLVDEIEQLRSSF 66
           +AE L+ L   R +  +L + P V+ESDALSV++ +  K   LSE+  ++ +I ++ +SF
Sbjct: 107 VAETLAILHGCRLA-AELGLLPTVLESDALSVVEAVRLKSMPLSEVGVVIQDILEIFTSF 166

Query: 67  GEFSFSFCPRSRNEHAHSLARSFLG 92
             FS  F PR  N+  H LAR  +G
Sbjct: 167 PVFSICFVPRLANKVVHGLARLAVG 190

BLAST of Spg019810 vs. ExPASy TrEMBL
Match: A0A7J8UY98 (Uncharacterized protein OS=Gossypium klotzschianum OX=34286 GN=Goklo_007711 PE=4 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 4.7e-04
Identity = 29/79 (36.71%), Postives = 43/79 (54.43%), Query Frame = 0

Query: 8   AEGLSCLPSIRASRPDLHVPPIVVESDALSVIKLLNKKDENLSEITFLVDEIEQLRSSFG 67
           AE ++C  + +  R D+    I++E DALS+IK    K  + S I   + +I QL  +FG
Sbjct: 229 AEAIACRTATQIGR-DMQWSNIIIEGDALSIIKKCKLKSHDKSRIGAYIQDIHQLLKNFG 288

Query: 68  EFSFSFCPRSRNEHAHSLA 87
            + F + PR  N  AH LA
Sbjct: 289 NYYFEYIPREANNLAHKLA 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA3477308.18.8e-0535.16reverse transcriptase [Gossypium australe][more]
KAA3466751.11.5e-0437.21reverse transcriptase [Gossypium australe][more]
XP_015383853.12.6e-0445.76uncharacterized protein LOC107176237 [Citrus sinensis][more]
MBA0841494.14.4e-0435.87hypothetical protein [Gossypium armourianum][more]
XP_015384866.17.5e-0442.37uncharacterized protein LOC107176592 [Citrus sinensis] >XP_024953719.1 uncharact... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5B6W8144.3e-0535.16Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_011206 PE=4 SV=1[more]
A0A5B6VCL17.3e-0537.21Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_001820 PE=4 SV=1[more]
A0A7J9K4Z72.1e-0435.87RNase H domain-containing protein (Fragment) OS=Gossypium armourianum OX=34283 G... [more]
A0A5C7ITL24.7e-0438.82RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_001058 ... [more]
A0A7J8UY984.7e-0436.71Uncharacterized protein OS=Gossypium klotzschianum OX=34286 GN=Goklo_007711 PE=4... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..92
e-value: 2.2E-9
score: 39.3
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 22..87
e-value: 7.3E-13
score: 48.4
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 3..88
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 3..88
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 2..90

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg019810.1Spg019810.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity