Spg019622 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg019622
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionsuccinate dehydrogenase assembly factor 2, mitochondrial
Locationscaffold5: 30923035 .. 30926548 (-)
RNA-Seq ExpressionSpg019622
SyntenySpg019622
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCAAATGTAAGAAGAACGGTGCTGACGTGACGGTTAAAAATAATAAAATAATATTTTTTAAATTAAAATAATAATAAACTTAATAATTAAATAAATTAATAAAAATAATAAGGGTTAGTTGGGGGTTTGGGAAATAGGGTTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAGAAAAGAAAAAAGAAAAAAGAAAAATGAGTGATTTTGGAAAATGGAGAAAGAAATGGGGAGTGATCTTGGAAAATGGAAAGAGAAAAGTGGAATGATTTGTGAAGTGGGGGTTTGGGAAATAGGGGTTTTGGAAAATGGGAGCCCAAACACCAACTTGGAAAAATGGGGTGTTTTCCAAAACCCCCACTTCCAAAATAGGGACCCCAAACACCCCCTAAAAGTTCCTTTGTGTTCTTTAGTTGCAAAAAGACGTTTTCCCAAGTCCGTTCATGCCACGCCGATCCATTCGTCTCACTTTCTCTGTTCACAATTAAATTTTCTGGTTTGCAGTTAGAAAGAATACTTTTTTTTTTTTTTTTTTAATTTTTTTTTTTAATATAAAATTATTTGAATCCAATCATCCTCCCCATTTTGAAAAACAGAATGGCGACTTTCAGAAGGGCTCTGATCAGAGCTCATAAAGTTCTACACTTGGAAACCTCCACCGTTTTGAGAAGCGAATCAATTCCCATCTTGTCTAGCGCTTTTATTAGATTGGAGAACCCACAAATCCGATCAGCCAATCACTATCAGAATCACCATGGTTATTTCTTTGGTTCTCGATGCTCTTCGACGATTGCGAATTCTCTACCGACAGATATCGATCTCTCAAATGAAGAGAGCAAGAGGCGCTTGGTCAATAGGTTCGATCTTGGCTTGTTTTATTTTGCTTAGTAACTTTCACATCCTTCTCGCTCTCTCTCTCAATTATTGCCAATTGACAAGTCATTTTTGGTTTTGGATTTAACAGGTTGCTGTACCGGAGCAAACAACGAGGGTTTCTGGAATTGGATTTGGTACTTGGGAAATGGGTAGAGGATCACATCAATTCCTTGGATGCAGATGGTATCAAGGCTCTTATTAATGTTCTTGACCTGGTAATTTAGTCTTCTTACCATATTTGGAGTGATTTTCTATGCTTTATAGTCCAGCTCTAGTTGGTTTTGATCCATAGCTACACAGTTCTTTCATTTCTTCTACTGGTTCAAAATATTGCTGATAGTTCATTCGTTTCGAGTATAGGTGGTGAATTTAAAGTGCTTTTCAATTCTCTTGCAATGGATTTTGCAACTCATTTTATCCATATTAAGGAGAGAAAGGCAGCTGGCTGCCTCTTTTCTGGGAATGGCAAAGGCAATACTGAATCCCCTGATTGACTTTTACTTGAATTTTCTTGCCCACCAGATTGTGGGCGTTTTGACCTCAAAGGGGTGTATATATACTTTATTGGACAACTGGTTAATTATTAATGCTTGTTTACTAATAACTTAATTCTAATCAGGATTTTTCATGTATAACAAAAACAATCCTCTACCAGTGTTCTGCATCTCATTTTTGTGTAACCCTTAAATCAAAGGTTGTCTTTTTGGAACCTCGTGAGTACCCATTCCTTTTACCTATGTGGATCCTCCTTCAGGAGTGTCGTCTTTCTAGAACCTAATGGTGTAGAGGCTTCGAGGACAGCTATTTAAGGCCAATACCCTGTTACAAAGGGAATGGTGGAGGAGATAGTTCCAACAAGTGTGAAAGTTACAACAACGAGTAGTAAAATTGAGGGGAAGGGTTTTAGTTTAGTGATTATGCCCCCATTTTTTTTTTATACAATAAACCGAACTTTCATTGAGAAAAAATGAAAGAATAAACGGGCATACAAAAGAAAAGCCCACAAAAAAAGAAAGGGAGCCACACTATAAAAAGGGACTCCAATCAAGCAAAATAATGCCTAAAGTGTAATTACACTGCAAAAGGACTTAGACATCGAGGCCCAAAGAGAAACATAGAATCTAACAAGGGACCAAATATCACTCCAAGAGCTCTCACGTCCTTTGAAGATTTTGTTGTTTCTCTCCCCCAAAGGCCCCATACAATCGCACAAACCTCAGCTTGCCACAAAAAGCATCCTCTATCGCTGAAGGACGGATAAAGAAGAAGCTCCTCGAATGCCATCCTAAAAGCTTGAGCTCTGACAAAACGATAGCCAAACTCCTGGAATATGTGGAATCTTTCACTAGATTTCAGTTTAGTCTATGTGTAGAGGTTGAGTATTTGTGAGATACTGGGGAGGTTATTCATGATCCCGGTTTGGGAGAGGGGTGGTCTTCTTAAGTAGGTGGAAAGGAATGTAAGAGTACTTCGTGTAGAAATAGTTTGTGGGAGAATATTTGGTAGTCGACATGTTTTAACCCTTCTCTTTGGCTCCCAATTCATATTTCTTTTGCAAATATTCTCTACCGTTTATTACGAAAAGTTGGGGTTTTTGTTAAACTGCTGTAAATGATTTTCAGGACTTATTTTTGGACTATGGTTAGTTTTCAGAAAGAAAAGCAACGACTGGAGTGGGAGCTTCTTTATGTGTTTTTCTCTATGATTTTTAATGAAAGTTGGGTTTTCATTGAAAAAAAAAAAAAGAATAGAGATGTGTGCATGAGAGGTCCGATTGTTAAATCCATTGATTAAGGTTATTTCGTATTAGATGCGTTCACATATTATGCTCACCTAGAATTAAAATTGAAAAATATTTCATTTTGGTCTCCTTTCCTTCTTGTTTCCCAATTGTTGTCTCGGTTTTTCATTCCAACATGAAACTTAGTGGGAGATATCCTCGCAGATTAATGAATCATTCTTGAAGTTCCTTATAAAACTCGTTAAGGGGAACTTTGAAAAGAAGTGGAAATTTGAGAAATGGCTCCCAAAAGTGTTAAAGAGATCATCCGTGAAGGAGAGTTGATCTTGGGAAGGAGTAGTCTAGCCCTCATGAGAGAAAAAGAAGATAGGGTGGTTCAGGGGAAGAGAGTTGGGGATTAGGTTAGAGCTATAAGAATTTTTGGATAAAGACATAATAAAGTTTTGAGTTCATTTGCTTTGTTTGATTATAAAGAAAGCCTCCAAAGTCCTGATAACTACCCATTGAAAATGAAAAATAGTATAGACAATAGAATAGATATACTTGCTAAATCTTCTGTTCTTCAGGAAAATCCAGATTTGTGGAAATGGTTAACTGGGCAGGAGCAACCCCCTGAAGCAGTGAAAACAAATCCTGTGAGACATCTAAGCGTGACACTACTGCTTTCCTTTTCTTTTGTCTTTGGACTAAATATGTGATTTACAAATGGAGTAACTATGTTTAATTTGTAGGTATTTATTGGTGTGCAAGAGAAGGTTATGAACAACCTCGACAGGCATGCCTCGCCCGAGACACGAACACCGCCTGGGCAACAATGGGTAAGGGGTTGGGATGATTTCAAGAGAGGTCGGGATGGCCCCATAACAGGAAATCAGTAG

mRNA sequence

ATGGCGACTTTCAGAAGGGCTCTGATCAGAGCTCATAAAGTTCTACACTTGGAAACCTCCACCGTTTTGAGAAGCGAATCAATTCCCATCTTGTCTAGCGCTTTTATTAGATTGGAGAACCCACAAATCCGATCAGCCAATCACTATCAGAATCACCATGGTTATTTCTTTGGTTCTCGATGCTCTTCGACGATTGCGAATTCTCTACCGACAGATATCGATCTCTCAAATGAAGAGAGCAAGAGGCGCTTGGTCAATAGGTTGCTGTACCGGAGCAAACAACGAGGGTTTCTGGAATTGGATTTGGTACTTGGGAAATGGGTAGAGGATCACATCAATTCCTTGGATGCAGATGGTATCAAGGCTCTTATTAATGTTCTTGACCTGGAAAATCCAGATTTGTGGAAATGGTTAACTGGGCAGGAGCAACCCCCTGAAGCAGTGAAAACAAATCCTGTATTTATTGGTGTGCAAGAGAAGGTTATGAACAACCTCGACAGGCATGCCTCGCCCGAGACACGAACACCGCCTGGGCAACAATGGGTAAGGGGTTGGGATGATTTCAAGAGAGGTCGGGATGGCCCCATAACAGGAAATCAGTAG

Coding sequence (CDS)

ATGGCGACTTTCAGAAGGGCTCTGATCAGAGCTCATAAAGTTCTACACTTGGAAACCTCCACCGTTTTGAGAAGCGAATCAATTCCCATCTTGTCTAGCGCTTTTATTAGATTGGAGAACCCACAAATCCGATCAGCCAATCACTATCAGAATCACCATGGTTATTTCTTTGGTTCTCGATGCTCTTCGACGATTGCGAATTCTCTACCGACAGATATCGATCTCTCAAATGAAGAGAGCAAGAGGCGCTTGGTCAATAGGTTGCTGTACCGGAGCAAACAACGAGGGTTTCTGGAATTGGATTTGGTACTTGGGAAATGGGTAGAGGATCACATCAATTCCTTGGATGCAGATGGTATCAAGGCTCTTATTAATGTTCTTGACCTGGAAAATCCAGATTTGTGGAAATGGTTAACTGGGCAGGAGCAACCCCCTGAAGCAGTGAAAACAAATCCTGTATTTATTGGTGTGCAAGAGAAGGTTATGAACAACCTCGACAGGCATGCCTCGCCCGAGACACGAACACCGCCTGGGCAACAATGGGTAAGGGGTTGGGATGATTTCAAGAGAGGTCGGGATGGCCCCATAACAGGAAATCAGTAG

Protein sequence

MATFRRALIRAHKVLHLETSTVLRSESIPILSSAFIRLENPQIRSANHYQNHHGYFFGSRCSSTIANSLPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADGIKALINVLDLENPDLWKWLTGQEQPPEAVKTNPVFIGVQEKVMNNLDRHASPETRTPPGQQWVRGWDDFKRGRDGPITGNQ
Homology
BLAST of Spg019622 vs. NCBI nr
Match: XP_022994486.1 (succinate dehydrogenase assembly factor 2, mitochondrial [Cucurbita maxima])

HSP 1 Score: 336.3 bits (861), Expect = 1.8e-88
Identity = 166/201 (82.59%), Postives = 179/201 (89.05%), Query Frame = 0

Query: 1   MATFRRALIRAHKVLHLE-TSTVLRSESIPILSSAFIRLENPQIRSANHYQNHHGYFFGS 60
           MATFRRAL++AH+VL+L+ +STV RS+S+P+L SA+I LEN Q R  NH QN HG FF  
Sbjct: 1   MATFRRALVKAHEVLYLKPSSTVFRSKSMPLLPSAYILLENLQGRLPNHNQNQHGCFFSF 60

Query: 61  RCSSTIANSLPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG 120
           RCSST  N L TDIDLSNEESKRRL NRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG
Sbjct: 61  RCSSTSVNPLFTDIDLSNEESKRRLFNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG 120

Query: 121 IKALINVLDLENPDLWKWLTGQEQPPEAVKTNPVFIGVQEKVMNNLDRHASPETRTPPGQ 180
           I ALI VLDLENPDLWKWLTGQEQPP+AVKTNPVF  VQEKVMNNL++HASPETRTPPGQ
Sbjct: 121 ISALIKVLDLENPDLWKWLTGQEQPPKAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQ 180

Query: 181 QWVRGWDDFKRGRDGPITGNQ 201
           QWVRGWDDFKRGRDGPITGNQ
Sbjct: 181 QWVRGWDDFKRGRDGPITGNQ 201

BLAST of Spg019622 vs. NCBI nr
Match: XP_022930034.1 (succinate dehydrogenase assembly factor 2, mitochondrial [Cucurbita moschata])

HSP 1 Score: 334.3 bits (856), Expect = 7.0e-88
Identity = 164/201 (81.59%), Postives = 180/201 (89.55%), Query Frame = 0

Query: 1   MATFRRALIRAHKVLHLE-TSTVLRSESIPILSSAFIRLENPQIRSANHYQNHHGYFFGS 60
           MATFRRA+++AH+VL+L+ +STV RS+S+P+L +A+I LEN Q R  NH QN HG FF  
Sbjct: 1   MATFRRAVLKAHQVLYLKPSSTVFRSKSMPLLPTAYILLENLQGRLPNHNQNQHGCFFSF 60

Query: 61  RCSSTIANSLPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG 120
           RCSST  N L TDIDLS+EESKRRL NRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG
Sbjct: 61  RCSSTTVNPLFTDIDLSDEESKRRLFNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG 120

Query: 121 IKALINVLDLENPDLWKWLTGQEQPPEAVKTNPVFIGVQEKVMNNLDRHASPETRTPPGQ 180
           I+ALI VLDLENPDLWKWLTGQEQPPEAVKTNPVF  VQEKVMNNL++HASPETRTPPGQ
Sbjct: 121 IRALIKVLDLENPDLWKWLTGQEQPPEAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQ 180

Query: 181 QWVRGWDDFKRGRDGPITGNQ 201
           QWVRGWDDFKRGRDGPITGNQ
Sbjct: 181 QWVRGWDDFKRGRDGPITGNQ 201

BLAST of Spg019622 vs. NCBI nr
Match: KAG7032455.1 (Succinate dehydrogenase assembly factor 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 334.3 bits (856), Expect = 7.0e-88
Identity = 164/201 (81.59%), Postives = 179/201 (89.05%), Query Frame = 0

Query: 1   MATFRRALIRAHKVLHLE-TSTVLRSESIPILSSAFIRLENPQIRSANHYQNHHGYFFGS 60
           MATFRRA+++AH+VL+L+ +STV RS+S+P+L +A+I LEN Q R  NH QN HG FF  
Sbjct: 1   MATFRRAVVKAHQVLYLKPSSTVFRSKSMPLLPTAYILLENLQGRLPNHNQNQHGCFFSF 60

Query: 61  RCSSTIANSLPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG 120
           RCSST  N L  DIDLSNEESKRRL NRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG
Sbjct: 61  RCSSTTVNPLFPDIDLSNEESKRRLFNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG 120

Query: 121 IKALINVLDLENPDLWKWLTGQEQPPEAVKTNPVFIGVQEKVMNNLDRHASPETRTPPGQ 180
           I+ALI VLDLENPDLWKWLTGQEQPPEAVKTNPVF  VQEKVMNNL++HASPETRTPPGQ
Sbjct: 121 IRALIKVLDLENPDLWKWLTGQEQPPEAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQ 180

Query: 181 QWVRGWDDFKRGRDGPITGNQ 201
           QWVRGWDDFKRGRDGPITGNQ
Sbjct: 181 QWVRGWDDFKRGRDGPITGNQ 201

BLAST of Spg019622 vs. NCBI nr
Match: XP_023530673.1 (succinate dehydrogenase assembly factor 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 327.8 bits (839), Expect = 6.6e-86
Identity = 162/201 (80.60%), Postives = 177/201 (88.06%), Query Frame = 0

Query: 1   MATFRRALIRAHKVLHLE-TSTVLRSESIPILSSAFIRLENPQIRSANHYQNHHGYFFGS 60
           MATFRRA+++AH+VL+L+ +STV RS+S+ +L +A+I LEN Q R  N  QN HG FF  
Sbjct: 1   MATFRRAVLKAHQVLYLKPSSTVFRSKSMTLLPTAYILLENLQGRLPNRNQNQHGSFFRF 60

Query: 61  RCSSTIANSLPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG 120
           RCSST  N   TDIDLSNEESKRRL NRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG
Sbjct: 61  RCSSTTVNPFFTDIDLSNEESKRRLFNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG 120

Query: 121 IKALINVLDLENPDLWKWLTGQEQPPEAVKTNPVFIGVQEKVMNNLDRHASPETRTPPGQ 180
           I+ALI VLDLENPDLWKWLTGQEQPPEAVKTNPVF  VQEKVMNNL++HASPETRTPPGQ
Sbjct: 121 IRALIKVLDLENPDLWKWLTGQEQPPEAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQ 180

Query: 181 QWVRGWDDFKRGRDGPITGNQ 201
           QWVRGWDDFKRGRDGPITGNQ
Sbjct: 181 QWVRGWDDFKRGRDGPITGNQ 201

BLAST of Spg019622 vs. NCBI nr
Match: KAG6601703.1 (Succinate dehydrogenase assembly factor 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 325.5 bits (833), Expect = 3.3e-85
Identity = 159/196 (81.12%), Postives = 174/196 (88.78%), Query Frame = 0

Query: 6   RALIRAHKVLHLE-TSTVLRSESIPILSSAFIRLENPQIRSANHYQNHHGYFFGSRCSST 65
           RA+++AH+VL+L+ +STV RS+S+P+L +A+I LEN Q R  NH QN HG FF  RCSST
Sbjct: 281 RAVVKAHQVLYLKPSSTVFRSKSMPLLPTAYILLENLQGRLPNHIQNQHGCFFSFRCSST 340

Query: 66  IANSLPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADGIKALI 125
             N L  DIDLSNEESKRRL NRLLYRSKQRGFLELDLVLGKWVEDHINSLDADGI+ALI
Sbjct: 341 TVNPLFPDIDLSNEESKRRLFNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADGIRALI 400

Query: 126 NVLDLENPDLWKWLTGQEQPPEAVKTNPVFIGVQEKVMNNLDRHASPETRTPPGQQWVRG 185
            VLDLENPDLWKWLTGQEQPPEAVKTNPVF  VQEKVMNNL++HASPETRTPPGQQWVRG
Sbjct: 401 KVLDLENPDLWKWLTGQEQPPEAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQQWVRG 460

Query: 186 WDDFKRGRDGPITGNQ 201
           WDDFKRGRDGPITGNQ
Sbjct: 461 WDDFKRGRDGPITGNQ 476

BLAST of Spg019622 vs. ExPASy Swiss-Prot
Match: Q9FI44 (Succinate dehydrogenase assembly factor 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SDHAF2 PE=1 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 3.5e-58
Identity = 122/202 (60.40%), Postives = 150/202 (74.26%), Query Frame = 0

Query: 1   MATFRRALIRAHKVLHLETSTVLRSESIPILSSAFIRLENPQIRSANHYQNHHGYFFGSR 60
           MAT R+ALI  H+++   T+ V RS  IP    A      P I     ++N  G   G+R
Sbjct: 1   MAT-RKALINVHRIIR-STAVVGRSSIIP----AAANRSYPII-----FRN--GVDLGAR 60

Query: 61  --CSSTIANSLPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDAD 120
             C +T A++   DIDLSNEE+KRR +NRLLYRSKQRGFLELDLVLG WVE+++NS+D +
Sbjct: 61  FFCENT-ASAQNFDIDLSNEENKRRTINRLLYRSKQRGFLELDLVLGNWVEENVNSMDEN 120

Query: 121 GIKALINVLDLENPDLWKWLTGQEQPPEAVKTNPVFIGVQEKVMNNLDRHASPETRTPPG 180
           G+K+LI+VL+LENPDLWKWLT QEQPPEAV +NPVF  + EKVM NL++HA+PETR   G
Sbjct: 121 GVKSLIHVLNLENPDLWKWLTEQEQPPEAVSSNPVFSALHEKVMKNLNKHAAPETRAAAG 180

Query: 181 QQWVRGWDDFKRGRDGPITGNQ 201
           Q WVRGWDDFKRGRD PI+GNQ
Sbjct: 181 QPWVRGWDDFKRGRDAPISGNQ 188

BLAST of Spg019622 vs. ExPASy Swiss-Prot
Match: P0CR33 (Succinate dehydrogenase assembly factor 2, mitochondrial OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=CNBA4490 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.8e-07
Identity = 29/95 (30.53%), Postives = 52/95 (54.74%), Query Frame = 0

Query: 69  LPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADGIKALINVLD 128
           LP  +D + E+ K  L  RL+Y++++RG LE DL+L  +  D + ++D + +K    +LD
Sbjct: 64  LPEPLDRTGEDEK-TLRARLIYQTRKRGTLETDLILSTFARDELPNMDFEEMKQFDKLLD 123

Query: 129 LENPDLWKWLTGQEQPPEAVKTNPVFIGVQEKVMN 164
             + D++ W   +  PP   K  P+   +Q+   N
Sbjct: 124 EPDWDIFYWSVKKRDPPARWKGTPLLEKLQKHAKN 157

BLAST of Spg019622 vs. ExPASy Swiss-Prot
Match: P0CR32 (Succinate dehydrogenase assembly factor 2, mitochondrial OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=CNA04680 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.8e-07
Identity = 29/95 (30.53%), Postives = 52/95 (54.74%), Query Frame = 0

Query: 69  LPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADGIKALINVLD 128
           LP  +D + E+ K  L  RL+Y++++RG LE DL+L  +  D + ++D + +K    +LD
Sbjct: 64  LPEPLDRTGEDEK-TLRARLIYQTRKRGTLETDLILSTFARDELPNMDFEEMKQFDKLLD 123

Query: 129 LENPDLWKWLTGQEQPPEAVKTNPVFIGVQEKVMN 164
             + D++ W   +  PP   K  P+   +Q+   N
Sbjct: 124 EPDWDIFYWSVKKRDPPARWKGTPLLEKLQKHAKN 157

BLAST of Spg019622 vs. ExPASy Swiss-Prot
Match: B6JZ70 (Succinate dehydrogenase assembly factor 2, mitochondrial OS=Schizosaccharomyces japonicus (strain yFS275 / FY16936) OX=402676 GN=SJAG_01895 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.1e-06
Identity = 27/85 (31.76%), Postives = 54/85 (63.53%), Query Frame = 0

Query: 62  SSTIANSLPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADGIK 121
           S  + + L   +D S +E+++ L +RL+Y+S++RG LE DL+L  + +++++  + + +K
Sbjct: 32  SKLLEDKLIRPVDRS-KETEKLLRSRLVYQSRKRGILETDLILSGFAKEYLSKYNVELLK 91

Query: 122 ALINVLDLENPDLWKWLTGQEQPPE 147
              N+L+  + D+  W TG+ Q PE
Sbjct: 92  EYDNLLNEADWDILYWCTGERQAPE 115

BLAST of Spg019622 vs. ExPASy Swiss-Prot
Match: Q5WWK6 (FAD assembly factor SdhE OS=Legionella pneumophila (strain Lens) OX=297245 GN=sdhE PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 3.1e-06
Identity = 26/59 (44.07%), Postives = 41/59 (69.49%), Query Frame = 0

Query: 86  NRLLYRSKQRGFLELDLVLGKWVEDHINSLDADGIKALINVLDLENPDLWKWLTGQEQP 145
           +RLL++ + RG LELDLVL K++ + I+ L  + +KA  N+L   +P+L+ WL G E+P
Sbjct: 7   SRLLWKCR-RGMLELDLVLQKFIANEIDRLTENQLKAFDNLLTHNDPNLYAWLMGHEEP 64

BLAST of Spg019622 vs. ExPASy TrEMBL
Match: A0A6J1JZA0 (succinate dehydrogenase assembly factor 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111490192 PE=4 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 8.9e-89
Identity = 166/201 (82.59%), Postives = 179/201 (89.05%), Query Frame = 0

Query: 1   MATFRRALIRAHKVLHLE-TSTVLRSESIPILSSAFIRLENPQIRSANHYQNHHGYFFGS 60
           MATFRRAL++AH+VL+L+ +STV RS+S+P+L SA+I LEN Q R  NH QN HG FF  
Sbjct: 1   MATFRRALVKAHEVLYLKPSSTVFRSKSMPLLPSAYILLENLQGRLPNHNQNQHGCFFSF 60

Query: 61  RCSSTIANSLPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG 120
           RCSST  N L TDIDLSNEESKRRL NRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG
Sbjct: 61  RCSSTSVNPLFTDIDLSNEESKRRLFNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG 120

Query: 121 IKALINVLDLENPDLWKWLTGQEQPPEAVKTNPVFIGVQEKVMNNLDRHASPETRTPPGQ 180
           I ALI VLDLENPDLWKWLTGQEQPP+AVKTNPVF  VQEKVMNNL++HASPETRTPPGQ
Sbjct: 121 ISALIKVLDLENPDLWKWLTGQEQPPKAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQ 180

Query: 181 QWVRGWDDFKRGRDGPITGNQ 201
           QWVRGWDDFKRGRDGPITGNQ
Sbjct: 181 QWVRGWDDFKRGRDGPITGNQ 201

BLAST of Spg019622 vs. ExPASy TrEMBL
Match: A0A6J1EQG7 (succinate dehydrogenase assembly factor 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111436468 PE=4 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 3.4e-88
Identity = 164/201 (81.59%), Postives = 180/201 (89.55%), Query Frame = 0

Query: 1   MATFRRALIRAHKVLHLE-TSTVLRSESIPILSSAFIRLENPQIRSANHYQNHHGYFFGS 60
           MATFRRA+++AH+VL+L+ +STV RS+S+P+L +A+I LEN Q R  NH QN HG FF  
Sbjct: 1   MATFRRAVLKAHQVLYLKPSSTVFRSKSMPLLPTAYILLENLQGRLPNHNQNQHGCFFSF 60

Query: 61  RCSSTIANSLPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG 120
           RCSST  N L TDIDLS+EESKRRL NRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG
Sbjct: 61  RCSSTTVNPLFTDIDLSDEESKRRLFNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG 120

Query: 121 IKALINVLDLENPDLWKWLTGQEQPPEAVKTNPVFIGVQEKVMNNLDRHASPETRTPPGQ 180
           I+ALI VLDLENPDLWKWLTGQEQPPEAVKTNPVF  VQEKVMNNL++HASPETRTPPGQ
Sbjct: 121 IRALIKVLDLENPDLWKWLTGQEQPPEAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQ 180

Query: 181 QWVRGWDDFKRGRDGPITGNQ 201
           QWVRGWDDFKRGRDGPITGNQ
Sbjct: 181 QWVRGWDDFKRGRDGPITGNQ 201

BLAST of Spg019622 vs. ExPASy TrEMBL
Match: A0A6J1C0N3 (succinate dehydrogenase assembly factor 2, mitochondrial isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007362 PE=4 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 1.0e-84
Identity = 153/200 (76.50%), Postives = 173/200 (86.50%), Query Frame = 0

Query: 1   MATFRRALIRAHKVLHLETSTVLRSESIPILSSAFIRLENPQIRSANHYQNHHGYFFGSR 60
           MATF RA+I+AH+VL++ET  +LR +++P +S AFI LEN Q R  +HYQ+ HG FF  R
Sbjct: 1   MATFIRAMIKAHRVLYMETPAILRRKTMPFMSGAFIGLENLQTRLTDHYQSQHGSFFSFR 60

Query: 61  CSSTIANSLPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADGI 120
            SST  N LPTDIDLSNEESKRRL NRLLYRS+QRG LELDL+LGKWVEDHI S+DADGI
Sbjct: 61  SSSTNKNPLPTDIDLSNEESKRRLFNRLLYRSRQRGLLELDLILGKWVEDHIKSMDADGI 120

Query: 121 KALINVLDLENPDLWKWLTGQEQPPEAVKTNPVFIGVQEKVMNNLDRHASPETRTPPGQQ 180
           KALI+VLDLENPDLWKWLTGQE+PPEAVKTNPVF  VQEKV+NNL+RHA+PETRTPPGQQ
Sbjct: 121 KALISVLDLENPDLWKWLTGQEKPPEAVKTNPVFAAVQEKVVNNLNRHAAPETRTPPGQQ 180

Query: 181 WVRGWDDFKRGRDGPITGNQ 201
           WVRGWDDFK GRD PI GNQ
Sbjct: 181 WVRGWDDFKSGRDSPIAGNQ 200

BLAST of Spg019622 vs. ExPASy TrEMBL
Match: A0A6J1C101 (uncharacterized protein LOC111007362 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007362 PE=4 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 1.0e-84
Identity = 153/200 (76.50%), Postives = 173/200 (86.50%), Query Frame = 0

Query: 1   MATFRRALIRAHKVLHLETSTVLRSESIPILSSAFIRLENPQIRSANHYQNHHGYFFGSR 60
           MATF RA+I+AH+VL++ET  +LR +++P +S AFI LEN Q R  +HYQ+ HG FF  R
Sbjct: 100 MATFIRAMIKAHRVLYMETPAILRRKTMPFMSGAFIGLENLQTRLTDHYQSQHGSFFSFR 159

Query: 61  CSSTIANSLPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADGI 120
            SST  N LPTDIDLSNEESKRRL NRLLYRS+QRG LELDL+LGKWVEDHI S+DADGI
Sbjct: 160 SSSTNKNPLPTDIDLSNEESKRRLFNRLLYRSRQRGLLELDLILGKWVEDHIKSMDADGI 219

Query: 121 KALINVLDLENPDLWKWLTGQEQPPEAVKTNPVFIGVQEKVMNNLDRHASPETRTPPGQQ 180
           KALI+VLDLENPDLWKWLTGQE+PPEAVKTNPVF  VQEKV+NNL+RHA+PETRTPPGQQ
Sbjct: 220 KALISVLDLENPDLWKWLTGQEKPPEAVKTNPVFAAVQEKVVNNLNRHAAPETRTPPGQQ 279

Query: 181 WVRGWDDFKRGRDGPITGNQ 201
           WVRGWDDFK GRD PI GNQ
Sbjct: 280 WVRGWDDFKSGRDSPIAGNQ 299

BLAST of Spg019622 vs. ExPASy TrEMBL
Match: A0A5D3C9Y9 (Succinate dehydrogenase assembly factor 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold648G001200 PE=4 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 4.5e-80
Identity = 153/201 (76.12%), Postives = 171/201 (85.07%), Query Frame = 0

Query: 1   MATFRRALIRAHKVLHLETS-TVLRSESIPILSSAFIRLENPQIRSANHYQNHHGYFFGS 60
           MATF RAL +AH+VL+L+ S T+LRS+ +P L S FI LEN Q +  N  QNHHG  F  
Sbjct: 3   MATFIRALSKAHQVLYLKASPTILRSKLMPFLPSVFIGLENLQSQLTNRNQNHHGCSFSL 62

Query: 61  RCSSTIANSLPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDADG 120
             SST  N    DIDLSNE+SKR+L NRLLYRSKQRGFLELDL+LGKWVEDHI+SLDADG
Sbjct: 63  HFSSTSTN---PDIDLSNEDSKRQLFNRLLYRSKQRGFLELDLILGKWVEDHIHSLDADG 122

Query: 121 IKALINVLDLENPDLWKWLTGQEQPPEAVKTNPVFIGVQEKVMNNLDRHASPETRTPPGQ 180
           I+ LINVLDLENPDLWKWLTGQEQPPEA+KTNPVF GV+EKVMNNL++HASPETRTPPGQ
Sbjct: 123 IRDLINVLDLENPDLWKWLTGQEQPPEALKTNPVFTGVKEKVMNNLNKHASPETRTPPGQ 182

Query: 181 QWVRGWDDFKRGRDGPITGNQ 201
           QWVRGWDDFK+GRDGPITGNQ
Sbjct: 183 QWVRGWDDFKKGRDGPITGNQ 200

BLAST of Spg019622 vs. TAIR 10
Match: AT5G51040.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF339 (InterPro:IPR005631); Has 532 Blast hits to 532 proteins in 207 species: Archae - 0; Bacteria - 285; Metazoa - 16; Fungi - 41; Plants - 40; Viruses - 0; Other Eukaryotes - 150 (source: NCBI BLink). )

HSP 1 Score: 226.1 bits (575), Expect = 2.5e-59
Identity = 122/202 (60.40%), Postives = 150/202 (74.26%), Query Frame = 0

Query: 1   MATFRRALIRAHKVLHLETSTVLRSESIPILSSAFIRLENPQIRSANHYQNHHGYFFGSR 60
           MAT R+ALI  H+++   T+ V RS  IP    A      P I     ++N  G   G+R
Sbjct: 1   MAT-RKALINVHRIIR-STAVVGRSSIIP----AAANRSYPII-----FRN--GVDLGAR 60

Query: 61  --CSSTIANSLPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDAD 120
             C +T A++   DIDLSNEE+KRR +NRLLYRSKQRGFLELDLVLG WVE+++NS+D +
Sbjct: 61  FFCENT-ASAQNFDIDLSNEENKRRTINRLLYRSKQRGFLELDLVLGNWVEENVNSMDEN 120

Query: 121 GIKALINVLDLENPDLWKWLTGQEQPPEAVKTNPVFIGVQEKVMNNLDRHASPETRTPPG 180
           G+K+LI+VL+LENPDLWKWLT QEQPPEAV +NPVF  + EKVM NL++HA+PETR   G
Sbjct: 121 GVKSLIHVLNLENPDLWKWLTEQEQPPEAVSSNPVFSALHEKVMKNLNKHAAPETRAAAG 180

Query: 181 QQWVRGWDDFKRGRDGPITGNQ 201
           Q WVRGWDDFKRGRD PI+GNQ
Sbjct: 181 QPWVRGWDDFKRGRDAPISGNQ 188

BLAST of Spg019622 vs. TAIR 10
Match: AT5G51040.3 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF339 (InterPro:IPR005631). )

HSP 1 Score: 218.4 bits (555), Expect = 5.2e-57
Identity = 122/211 (57.82%), Postives = 150/211 (71.09%), Query Frame = 0

Query: 1   MATFRRALIRAHKVLHLETSTVLRSESIPILSSAFIRLENPQIRSANHYQNHHGYFFGSR 60
           MAT R+ALI  H+++   T+ V RS  IP    A      P I     ++N  G   G+R
Sbjct: 1   MAT-RKALINVHRIIR-STAVVGRSSIIP----AAANRSYPII-----FRN--GVDLGAR 60

Query: 61  --CSSTIANSLPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDAD 120
             C +T A++   DIDLSNEE+KRR +NRLLYRSKQRGFLELDLVLG WVE+++NS+D +
Sbjct: 61  FFCENT-ASAQNFDIDLSNEENKRRTINRLLYRSKQRGFLELDLVLGNWVEENVNSMDEN 120

Query: 121 GIKALINVLD---------LENPDLWKWLTGQEQPPEAVKTNPVFIGVQEKVMNNLDRHA 180
           G+K+LI+VL+         LENPDLWKWLT QEQPPEAV +NPVF  + EKVM NL++HA
Sbjct: 121 GVKSLIHVLNLVESEATKLLENPDLWKWLTEQEQPPEAVSSNPVFSALHEKVMKNLNKHA 180

Query: 181 SPETRTPPGQQWVRGWDDFKRGRDGPITGNQ 201
           +PETR   GQ WVRGWDDFKRGRD PI+GNQ
Sbjct: 181 APETRAAAGQPWVRGWDDFKRGRDAPISGNQ 197

BLAST of Spg019622 vs. TAIR 10
Match: AT5G51040.2 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF339 (InterPro:IPR005631); Has 368 Blast hits to 368 proteins in 141 species: Archae - 0; Bacteria - 216; Metazoa - 8; Fungi - 13; Plants - 40; Viruses - 0; Other Eukaryotes - 91 (source: NCBI BLink). )

HSP 1 Score: 212.6 bits (540), Expect = 2.9e-55
Identity = 118/202 (58.42%), Postives = 146/202 (72.28%), Query Frame = 0

Query: 1   MATFRRALIRAHKVLHLETSTVLRSESIPILSSAFIRLENPQIRSANHYQNHHGYFFGSR 60
           MAT R+ALI  H+++   T+ V RS  IP    A      P I     ++N  G   G+R
Sbjct: 1   MAT-RKALINVHRIIR-STAVVGRSSIIP----AAANRSYPII-----FRN--GVDLGAR 60

Query: 61  --CSSTIANSLPTDIDLSNEESKRRLVNRLLYRSKQRGFLELDLVLGKWVEDHINSLDAD 120
             C +T A++   DIDLSNEE+KRR +N    RSKQRGFLELDLVLG WVE+++NS+D +
Sbjct: 61  FFCENT-ASAQNFDIDLSNEENKRRTIN----RSKQRGFLELDLVLGNWVEENVNSMDEN 120

Query: 121 GIKALINVLDLENPDLWKWLTGQEQPPEAVKTNPVFIGVQEKVMNNLDRHASPETRTPPG 180
           G+K+LI+VL+LENPDLWKWLT QEQPPEAV +NPVF  + EKVM NL++HA+PETR   G
Sbjct: 121 GVKSLIHVLNLENPDLWKWLTEQEQPPEAVSSNPVFSALHEKVMKNLNKHAAPETRAAAG 180

Query: 181 QQWVRGWDDFKRGRDGPITGNQ 201
           Q WVRGWDDFKRGRD PI+GNQ
Sbjct: 181 QPWVRGWDDFKRGRDAPISGNQ 184

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022994486.11.8e-8882.59succinate dehydrogenase assembly factor 2, mitochondrial [Cucurbita maxima][more]
XP_022930034.17.0e-8881.59succinate dehydrogenase assembly factor 2, mitochondrial [Cucurbita moschata][more]
KAG7032455.17.0e-8881.59Succinate dehydrogenase assembly factor 2, mitochondrial [Cucurbita argyrosperma... [more]
XP_023530673.16.6e-8680.60succinate dehydrogenase assembly factor 2, mitochondrial [Cucurbita pepo subsp. ... [more]
KAG6601703.13.3e-8581.12Succinate dehydrogenase assembly factor 2, mitochondrial, partial [Cucurbita arg... [more]
Match NameE-valueIdentityDescription
Q9FI443.5e-5860.40Succinate dehydrogenase assembly factor 2, mitochondrial OS=Arabidopsis thaliana... [more]
P0CR332.8e-0730.53Succinate dehydrogenase assembly factor 2, mitochondrial OS=Cryptococcus neoform... [more]
P0CR322.8e-0730.53Succinate dehydrogenase assembly factor 2, mitochondrial OS=Cryptococcus neoform... [more]
B6JZ701.1e-0631.76Succinate dehydrogenase assembly factor 2, mitochondrial OS=Schizosaccharomyces ... [more]
Q5WWK63.1e-0644.07FAD assembly factor SdhE OS=Legionella pneumophila (strain Lens) OX=297245 GN=sd... [more]
Match NameE-valueIdentityDescription
A0A6J1JZA08.9e-8982.59succinate dehydrogenase assembly factor 2, mitochondrial OS=Cucurbita maxima OX=... [more]
A0A6J1EQG73.4e-8881.59succinate dehydrogenase assembly factor 2, mitochondrial OS=Cucurbita moschata O... [more]
A0A6J1C0N31.0e-8476.50succinate dehydrogenase assembly factor 2, mitochondrial isoform X2 OS=Momordica... [more]
A0A6J1C1011.0e-8476.50uncharacterized protein LOC111007362 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A5D3C9Y94.5e-8076.12Succinate dehydrogenase assembly factor 2 OS=Cucumis melo var. makuwa OX=1194695... [more]
Match NameE-valueIdentityDescription
AT5G51040.12.5e-5960.40unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF339 ... [more]
AT5G51040.35.2e-5757.82unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT5G51040.22.9e-5558.42unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF339 ... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036714Flavinator of succinate dehydrogenase superfamilyGENE3D1.10.150.250Flavinator of succinate dehydrogenasecoord: 71..167
e-value: 6.4E-22
score: 79.0
IPR036714Flavinator of succinate dehydrogenase superfamilySUPERFAMILY109910YgfY-likecoord: 84..151
IPR005631Flavinator of succinate dehydrogenasePFAMPF03937Sdh5coord: 86..154
e-value: 1.4E-21
score: 76.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 167..200
NoneNo IPR availablePANTHERPTHR12469PROTEIN EMI5 HOMOLOG, MITOCHONDRIALcoord: 2..200
NoneNo IPR availablePANTHERPTHR12469:SF5FLAVINATOR OF SUCCINATE DEHYDROGENASEcoord: 2..200

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg019622.1Spg019622.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006121 mitochondrial electron transport, succinate to ubiquinone
biological_process GO:0034553 mitochondrial respiratory chain complex II assembly
biological_process GO:0018293 protein-FAD linkage
biological_process GO:0006099 tricarboxylic acid cycle
cellular_component GO:0005739 mitochondrion