Spg018374 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg018374
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionagamous-like MADS-box protein AGL62
Locationscaffold3: 10265476 .. 10266741 (-)
RNA-Seq ExpressionSpg018374
SyntenySpg018374
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATTTGATAGGAAAAGTGTGGGAAAGAGGTGAGAAATTGACGGCGGTCAGAATCGGAGTGTCTTGGTACTACCGTTGCCTCCACCGTCGAATGCTTCGTCAACGTCGTTTCACCACACCGAAAATCACCAAAATTTTCTCTCTTCTACGATGTTTCATCTTGCTAGAAACCCCGATGTCCCGATGGGGTTAGGATTTTTCTACATTTTATTCATTAATCTATTTTGATTACTATACTTTATAGTTTTCTTTTTTAAATTTTAAGTGTTTTTGTAGCAACCCTGTTGTAAATATATATATATATACACATATATGTATGTATGTATTATGTATATATGTACTTGCCATAATTGAAATATAATTTGAATTCGTTTGTAAAGAACCAGAAGCTTCGTGTTCGTCTTTAATGGAGGAACTAAAATTGCAAAGAAAATCGATTGCGATGAAGAAAACCCTAAGCCGTCAGAATATTTAAATCAAGAAGTTAGAGAAGAAAAGCAGTAAAGAAGTCACCTTTTCGAAGCACCGTGCTGGACTTATCAAGAATGACGTCTAGGTCTCTATTTATTGCGGAGCAAAGGTTGCCATCATCGTCTTGTATCCCAACGGTAAGGTGTTTTCCTTCAGTCATTCTGACGATGATCTGCTTCTGGATCACTTTCTCATTGAAATCTTGTCGATGTCGAAGCCGGGTGAGAGATACGTCTCTATGGCGTTGTTTAACTGCGATTTTGTCGATTTAGCGGTAGAACTGGAAGCAGAGAAGAAGCTAGAGACAAAGTTGATAGAGACGATGGAGGATTTCAGGAAGAACGAAGGATTCCGGTGGGAGTAAACGATTGAAGGGATGAGATCGGAGGAATTGAGGGAATTTTATATTGGAGTTGATGTATTTGAGAGGCAAAGATGAAGGATTGGAATTTCGTAATTCCATAAAGCGGAAGCGTGATTGGGACCGATTCGTAATTAATTTTGTTTAATCTAAAATACCAATACATTGGCAATAATATTAGAACTATTCTAAATATATATTAAGGCTAAGCATACAAAATATTAGATCTAAATCTAATTTATAAAGTAACAAATTACTTACTCTCGTTGACGACTCAGATTAAATTCACCAACTGCTTGTGAATTAATCACGAACTAGTGCGAACACCACCACTATGGCTACACCGGTATGACTCTGAGACATCTAGAGACAGGAGACTGGTGGGAGTCTAGAGGAAGCCTTGAGGGAAATTCTCTGAGAGTAGGAGAATGA

mRNA sequence

ATGTATTTGATAGGAAAAGTGTGGGAAAGAGGTGAGAAATTGACGGCGGTCAGAATCGGAGTCTCTATTTATTGCGGAGCAAAGGTTGCCATCATCGTCTTGTATCCCAACGGTAAGGTGTTTTCCTTCAGTCATTCTGACGATGATCTGCTTCTGGATCACTTTCTCATTGAAATCTTGTCGATGTCGAAGCCGGGTGAGAGATACGTCTCTATGGCGTTGTTTAACTGCGATTTTGTCGATTTAGCGGTAGAACTGGAAGCAGAGAAGAAGCTAGAGACAAAGTTGATAGAGACGATGGAGGATTTCAGGAAGAACGAAGGATTCCGAGGCAAAGATGAAGGATTGGAATTTCGTAATTCCATAAAGCGGAAGCGTGATTGGGACCGATTCTGCGAACACCACCACTATGGCTACACCGGTATGACTCTGAGACATCTAGAGACAGGAGACTGGTGGGAGTCTAGAGGAAGCCTTGAGGGAAATTCTCTGAGAGTAGGAGAATGA

Coding sequence (CDS)

ATGTATTTGATAGGAAAAGTGTGGGAAAGAGGTGAGAAATTGACGGCGGTCAGAATCGGAGTCTCTATTTATTGCGGAGCAAAGGTTGCCATCATCGTCTTGTATCCCAACGGTAAGGTGTTTTCCTTCAGTCATTCTGACGATGATCTGCTTCTGGATCACTTTCTCATTGAAATCTTGTCGATGTCGAAGCCGGGTGAGAGATACGTCTCTATGGCGTTGTTTAACTGCGATTTTGTCGATTTAGCGGTAGAACTGGAAGCAGAGAAGAAGCTAGAGACAAAGTTGATAGAGACGATGGAGGATTTCAGGAAGAACGAAGGATTCCGAGGCAAAGATGAAGGATTGGAATTTCGTAATTCCATAAAGCGGAAGCGTGATTGGGACCGATTCTGCGAACACCACCACTATGGCTACACCGGTATGACTCTGAGACATCTAGAGACAGGAGACTGGTGGGAGTCTAGAGGAAGCCTTGAGGGAAATTCTCTGAGAGTAGGAGAATGA

Protein sequence

MYLIGKVWERGEKLTAVRIGVSIYCGAKVAIIVLYPNGKVFSFSHSDDDLLLDHFLIEILSMSKPGERYVSMALFNCDFVDLAVELEAEKKLETKLIETMEDFRKNEGFRGKDEGLEFRNSIKRKRDWDRFCEHHHYGYTGMTLRHLETGDWWESRGSLEGNSLRVGE
Homology
BLAST of Spg018374 vs. NCBI nr
Match: XP_008466532.1 (PREDICTED: agamous-like MADS-box protein AGL62 [Cucumis melo])

HSP 1 Score: 96.7 bits (239), Expect = 2.1e-16
Identity = 50/89 (56.18%), Postives = 59/89 (66.29%), Query Frame = 0

Query: 21  VSIYCGAKVAIIVLYPNGKVFSFSHSDDDLLLDHFLIEILSMSKPGERYVSMALFNCDFV 80
           +S+ CGA+VAIIV  PN KVF F H D D+LLD +L   LS  KP E Y+ +A FN DF 
Sbjct: 47  LSVLCGAEVAIIVFSPNDKVFCFGHPDVDVLLDRYLTGNLSPPKPAESYIPVAEFNRDFA 106

Query: 81  DLAVELEAEKKLETKLIETMEDFRKNEGF 110
           D A+E EAEKK   +LI   ED RKN GF
Sbjct: 107 DFALEFEAEKKRAAELIRAAEDSRKNGGF 135

BLAST of Spg018374 vs. NCBI nr
Match: KAA0041553.1 (agamous-like MADS-box protein AGL62 [Cucumis melo var. makuwa] >TYK19701.1 agamous-like MADS-box protein AGL62 [Cucumis melo var. makuwa])

HSP 1 Score: 96.7 bits (239), Expect = 2.1e-16
Identity = 50/89 (56.18%), Postives = 59/89 (66.29%), Query Frame = 0

Query: 21  VSIYCGAKVAIIVLYPNGKVFSFSHSDDDLLLDHFLIEILSMSKPGERYVSMALFNCDFV 80
           +S+ CGA+VAIIV  PN KVF F H D D+LLD +L   LS  KP E Y+ +A FN DF 
Sbjct: 39  LSVLCGAEVAIIVFSPNDKVFCFGHPDVDVLLDRYLTGNLSPPKPAESYIPVAEFNRDFA 98

Query: 81  DLAVELEAEKKLETKLIETMEDFRKNEGF 110
           D A+E EAEKK   +LI   ED RKN GF
Sbjct: 99  DFALEFEAEKKRAAELIRAAEDSRKNGGF 127

BLAST of Spg018374 vs. NCBI nr
Match: XP_004147877.3 (agamous-like MADS-box protein AGL62 [Cucumis sativus] >KGN59988.1 hypothetical protein Csa_002516 [Cucumis sativus])

HSP 1 Score: 95.1 bits (235), Expect = 6.0e-16
Identity = 49/89 (55.06%), Postives = 59/89 (66.29%), Query Frame = 0

Query: 21  VSIYCGAKVAIIVLYPNGKVFSFSHSDDDLLLDHFLIEILSMSKPGERYVSMALFNCDFV 80
           +S+ CGA+VAIIV  PN K+F F H D D+LLD +L   LS  KP E Y+ +A FN DF 
Sbjct: 47  LSVLCGAEVAIIVFSPNDKLFCFGHPDVDVLLDRYLTGNLSPPKPAESYIPVAEFNRDFA 106

Query: 81  DLAVELEAEKKLETKLIETMEDFRKNEGF 110
           D A+E EAEKK   +LI   ED RKN GF
Sbjct: 107 DCALEFEAEKKRAAELIRAAEDSRKNGGF 135

BLAST of Spg018374 vs. NCBI nr
Match: KAG7013413.1 (Agamous-like MADS-box protein AGL62, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 94.0 bits (232), Expect = 1.3e-15
Identity = 55/110 (50.00%), Postives = 70/110 (63.64%), Query Frame = 0

Query: 21  VSIYCGAKVAIIVLYPNGKVFSFSHSDDDLLLDHFLIEILSMSKPGERYVSMALFNCDFV 80
           +S+ CGA+VAIIV  PN KVF F H D D+LLD +L   LS  KP E YV +A FN DF 
Sbjct: 51  LSVLCGAEVAIIVFSPNDKVFCFGHPDVDVLLDRYLTGNLSPPKPAESYVPVAEFNRDFA 110

Query: 81  DLAVELEAEKKLETKLIETMEDFRKNEGFRGKD--EGL------EFRNSI 123
           DL +E EAEKK   +LI+  E+ R + GF  ++  EGL      EFR+S+
Sbjct: 111 DLVLEFEAEKKRAEELIQASEESRNSGGFWWEEAVEGLRLEELKEFRSSL 160

BLAST of Spg018374 vs. NCBI nr
Match: XP_023549141.1 (agamous-like MADS-box protein AGL62 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 93.2 bits (230), Expect = 2.3e-15
Identity = 54/110 (49.09%), Postives = 70/110 (63.64%), Query Frame = 0

Query: 21  VSIYCGAKVAIIVLYPNGKVFSFSHSDDDLLLDHFLIEILSMSKPGERYVSMALFNCDFV 80
           +S+ CGA+VAIIV  PN KVF F H D D+LLD +L   LS  KP E YV +A FN DF 
Sbjct: 39  LSVLCGAEVAIIVFSPNDKVFCFGHPDVDVLLDRYLTGNLSPPKPAESYVPVAEFNRDFA 98

Query: 81  DLAVELEAEKKLETKLIETMEDFRKNEGFRGKD--EGL------EFRNSI 123
           DL +E EAEKK   +LI+  E+ R + GF  ++  EG+      EFR+S+
Sbjct: 99  DLVLEFEAEKKRAEELIQASEESRNSGGFWWEEAVEGMRLEELKEFRSSL 148

BLAST of Spg018374 vs. ExPASy Swiss-Prot
Match: O80807 (Agamous-like MADS-box protein AGL23 OS=Arabidopsis thaliana OX=3702 GN=AGL23 PE=2 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 5.0e-05
Identity = 27/71 (38.03%), Postives = 41/71 (57.75%), Query Frame = 0

Query: 25  CGAKVAIIVLYPNGKVFSFSHSDDDLLLDHFLIEILSMSKPG--ERYVSM--ALFNCDFV 84
           C AK+A+IV  P GKVFSF H + D+LLDHF   ++  +     E Y  +   + N  + 
Sbjct: 44  CDAKIAMIVFSPAGKVFSFGHPNVDVLLDHFRGCVVGHNNTNLDESYTKLHVQMLNKSYT 103

Query: 85  DLAVELEAEKK 92
           ++  E+E E+K
Sbjct: 104 EVKAEVEKEQK 114

BLAST of Spg018374 vs. ExPASy TrEMBL
Match: A0A5D3D807 (Agamous-like MADS-box protein AGL62 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold242G001020 PE=4 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.0e-16
Identity = 50/89 (56.18%), Postives = 59/89 (66.29%), Query Frame = 0

Query: 21  VSIYCGAKVAIIVLYPNGKVFSFSHSDDDLLLDHFLIEILSMSKPGERYVSMALFNCDFV 80
           +S+ CGA+VAIIV  PN KVF F H D D+LLD +L   LS  KP E Y+ +A FN DF 
Sbjct: 39  LSVLCGAEVAIIVFSPNDKVFCFGHPDVDVLLDRYLTGNLSPPKPAESYIPVAEFNRDFA 98

Query: 81  DLAVELEAEKKLETKLIETMEDFRKNEGF 110
           D A+E EAEKK   +LI   ED RKN GF
Sbjct: 99  DFALEFEAEKKRAAELIRAAEDSRKNGGF 127

BLAST of Spg018374 vs. ExPASy TrEMBL
Match: A0A1S3CRI8 (agamous-like MADS-box protein AGL62 OS=Cucumis melo OX=3656 GN=LOC103503920 PE=4 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.0e-16
Identity = 50/89 (56.18%), Postives = 59/89 (66.29%), Query Frame = 0

Query: 21  VSIYCGAKVAIIVLYPNGKVFSFSHSDDDLLLDHFLIEILSMSKPGERYVSMALFNCDFV 80
           +S+ CGA+VAIIV  PN KVF F H D D+LLD +L   LS  KP E Y+ +A FN DF 
Sbjct: 47  LSVLCGAEVAIIVFSPNDKVFCFGHPDVDVLLDRYLTGNLSPPKPAESYIPVAEFNRDFA 106

Query: 81  DLAVELEAEKKLETKLIETMEDFRKNEGF 110
           D A+E EAEKK   +LI   ED RKN GF
Sbjct: 107 DFALEFEAEKKRAAELIRAAEDSRKNGGF 135

BLAST of Spg018374 vs. ExPASy TrEMBL
Match: A0A0A0LE01 (MADS-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G860240 PE=4 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 2.9e-16
Identity = 49/89 (55.06%), Postives = 59/89 (66.29%), Query Frame = 0

Query: 21  VSIYCGAKVAIIVLYPNGKVFSFSHSDDDLLLDHFLIEILSMSKPGERYVSMALFNCDFV 80
           +S+ CGA+VAIIV  PN K+F F H D D+LLD +L   LS  KP E Y+ +A FN DF 
Sbjct: 47  LSVLCGAEVAIIVFSPNDKLFCFGHPDVDVLLDRYLTGNLSPPKPAESYIPVAEFNRDFA 106

Query: 81  DLAVELEAEKKLETKLIETMEDFRKNEGF 110
           D A+E EAEKK   +LI   ED RKN GF
Sbjct: 107 DCALEFEAEKKRAAELIRAAEDSRKNGGF 135

BLAST of Spg018374 vs. ExPASy TrEMBL
Match: A0A6J1H4M6 (agamous-like MADS-box protein AGL62 OS=Cucurbita moschata OX=3662 GN=LOC111460353 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 1.1e-15
Identity = 54/110 (49.09%), Postives = 70/110 (63.64%), Query Frame = 0

Query: 21  VSIYCGAKVAIIVLYPNGKVFSFSHSDDDLLLDHFLIEILSMSKPGERYVSMALFNCDFV 80
           +S+ CGA+VAIIV  PN KVF F H D D+LLD +L   LS  KP E YV +A FN DF 
Sbjct: 51  LSVLCGAEVAIIVFSPNDKVFCFGHPDVDVLLDRYLTGNLSPPKPAESYVPVAEFNRDFA 110

Query: 81  DLAVELEAEKKLETKLIETMEDFRKNEGFRGKD--EGL------EFRNSI 123
           DL +E EAEKK   +LI+  E+ R + GF  ++  EG+      EFR+S+
Sbjct: 111 DLVLEFEAEKKRAEELIQASEESRNSGGFWWEEAVEGMRLEELKEFRSSL 160

BLAST of Spg018374 vs. ExPASy TrEMBL
Match: A0A6J1KVC9 (agamous-like MADS-box protein AGL62 OS=Cucurbita maxima OX=3661 GN=LOC111499040 PE=4 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 4.2e-15
Identity = 53/110 (48.18%), Postives = 70/110 (63.64%), Query Frame = 0

Query: 21  VSIYCGAKVAIIVLYPNGKVFSFSHSDDDLLLDHFLIEILSMSKPGERYVSMALFNCDFV 80
           +S+ CGA+VAIIV  PN KVF F H + D+LLD +L   LS  KP E YV +A FN DF 
Sbjct: 51  LSVLCGAEVAIIVFSPNDKVFCFGHPNVDVLLDRYLTGNLSPPKPAESYVPVAEFNRDFA 110

Query: 81  DLAVELEAEKKLETKLIETMEDFRKNEGFRGKD--EGL------EFRNSI 123
           DL +E EAEKK   +LI+  E+ R + GF  ++  EG+      EFR+S+
Sbjct: 111 DLVLEFEAEKKRAEELIQASEESRNSGGFWWEEAVEGMRLEELKEFRSSL 160

BLAST of Spg018374 vs. TAIR 10
Match: AT1G65360.1 (AGAMOUS-like 23 )

HSP 1 Score: 49.3 bits (116), Expect = 3.5e-06
Identity = 27/71 (38.03%), Postives = 41/71 (57.75%), Query Frame = 0

Query: 25  CGAKVAIIVLYPNGKVFSFSHSDDDLLLDHFLIEILSMSKPG--ERYVSM--ALFNCDFV 84
           C AK+A+IV  P GKVFSF H + D+LLDHF   ++  +     E Y  +   + N  + 
Sbjct: 44  CDAKIAMIVFSPAGKVFSFGHPNVDVLLDHFRGCVVGHNNTNLDESYTKLHVQMLNKSYT 103

Query: 85  DLAVELEAEKK 92
           ++  E+E E+K
Sbjct: 104 EVKAEVEKEQK 114

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008466532.12.1e-1656.18PREDICTED: agamous-like MADS-box protein AGL62 [Cucumis melo][more]
KAA0041553.12.1e-1656.18agamous-like MADS-box protein AGL62 [Cucumis melo var. makuwa] >TYK19701.1 agamo... [more]
XP_004147877.36.0e-1655.06agamous-like MADS-box protein AGL62 [Cucumis sativus] >KGN59988.1 hypothetical p... [more]
KAG7013413.11.3e-1550.00Agamous-like MADS-box protein AGL62, partial [Cucurbita argyrosperma subsp. argy... [more]
XP_023549141.12.3e-1549.09agamous-like MADS-box protein AGL62 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
O808075.0e-0538.03Agamous-like MADS-box protein AGL23 OS=Arabidopsis thaliana OX=3702 GN=AGL23 PE=... [more]
Match NameE-valueIdentityDescription
A0A5D3D8071.0e-1656.18Agamous-like MADS-box protein AGL62 OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3CRI81.0e-1656.18agamous-like MADS-box protein AGL62 OS=Cucumis melo OX=3656 GN=LOC103503920 PE=4... [more]
A0A0A0LE012.9e-1655.06MADS-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G860240 PE... [more]
A0A6J1H4M61.1e-1549.09agamous-like MADS-box protein AGL62 OS=Cucurbita moschata OX=3662 GN=LOC11146035... [more]
A0A6J1KVC94.2e-1548.18agamous-like MADS-box protein AGL62 OS=Cucurbita maxima OX=3661 GN=LOC111499040 ... [more]
Match NameE-valueIdentityDescription
AT1G65360.13.5e-0638.03AGAMOUS-like 23 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002100Transcription factor, MADS-boxPFAMPF00319SRF-TFcoord: 22..43
e-value: 2.4E-4
score: 20.6
IPR036879Transcription factor, MADS-box superfamilyGENE3D3.40.1810.10coord: 20..64
e-value: 2.1E-5
score: 26.3
IPR036879Transcription factor, MADS-box superfamilySUPERFAMILY55455SRF-likecoord: 22..55

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg018374.1Spg018374.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0110165 cellular anatomical entity
molecular_function GO:0003677 DNA binding
molecular_function GO:0046983 protein dimerization activity