Spg017051 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg017051
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRhamnogalacturonan endolyase
Locationscaffold9: 41867094 .. 41872906 (+)
RNA-Seq ExpressionSpg017051
SyntenySpg017051
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCACCGTGTTATGTTTAAGTTGGCCGATTATTCAAACTCTAAAAACTAGTAAATTGGGAACGTTGCCTTGGCATTCCAAACACCTTCATCCATATAAATATCCGATCACCTCGCCAACTACGCGCGCCAGTTCCGTGCAGCACTAAATTCTAATCTTCGGCGTCGAGCAAAGTAATTCTCAATTGTTAACAGAATTAGAAAAGTCATTGTAAAGTGTCGATCATCGGAATCCATTTCATACCTAAAAACAAAGTGGAGAAACAATCCATTTTGTTCTCAGTCGATCGTTGTTCCCAATAAATTAGTATGTGCTCTCATTGCTCGTCTCCATCAGTCTCCGTCATCACAACAAACGGTTCTTCTTTTCTGTTGTTTGAGTTTTGATTTCGCCTTTCAATCTTCCAATTCTGTGTGTTCCACTGACTCTTGTTGCTCAAGAAATTTCAAAAGTGAAGGTTTCACAATGTCGTCCAGGGGAGTGCGATTGCAGATACGAGATCGTCATGTAAGTTAGCTTTTCCATTTTGTTCTAATTCTGTGAAGATATTCCAGAAGAAAATCACGAAAGGGAAGACTATGTATAATTGAAGATTGAAGAGAAGAAATGTTGTAATTTCCTTCCAGAACTCTGCAGATTATTCCTTGTTGATTCATGCTTTGTGCAGGTGGTGGTGGATAATGGAATACTGCAACTAACTTTATCAAAACCAGATGGAATCATCACTGGCATACGATATAATGGTGTTGACAATCTGCTTGAAGTTCTCAATCCTGAATCTAACAGAGGGTATACGCTGTTATAATCGTCACACCTCTGAAATTAATTTAATTAACAAAGAGTTGAAGATGACAATCTGCTTGTTTTTGTTATGAACATTCAGGTATTGGGATCTCGTTTGGAATTCTCCAGGAAGCAAGGGAATATTTGACGTGTATGGCCTTTTTCTTCTTTACTAGATCAATCATTGCAGATATTGGGGCATCGATACTATAATTTTCTCTGTTTTGTAGCATTTGATTGGTTTTGTTATTAGTTTATGTTTTCATGTAAATTGAGAGCCAATTTCCATACAGGATTAAAGGATCAAGTTTTAGGGTTATAGTGGAAAATGAGGAACAGGTTGAGCTTTCTTTTTTGAGGATGTGGGATCCCTCCCTCGAGGGAAAATTTGTTCCTTTGAATATCGACAAAAGGTTTGTCCTCATTTTTTTTTAATCGTTGTACTCGATCTTTCGATTGATTACTCAATTGAGCATTGCTGTTTTCTATTCTAGGTTCATAGTGCTACGTGGTTCCTCTGGTTTCTACTCTTATGCTATTTATGAGCACTTGAAGGATTGGCCTGATTTTGATATTGGTGAAACCAGGATCACTTTTAAACTCAGAAAAGACAAGTGAGTACACTTGATCCTTTGCCAGTCATTTATGATTTCATCGAGACTCGGTATAGTGCTTCAAGCTAGGACTTGTTTATCGAGTTGCTTAATTTCTCTTAGTTGGGTTATTTTCTTTCATGCCAACATATTATTCATTAGACCAAGGATTGAATTGATAGGTTTCAGTACATGGCTGTAGCTGACAACAGGCGGAGATTGATGCCCCTGCCCGATGATCGGTTATCTGGAAGTTGTCAAACTTTGGCCTACCCGGAAGCTGTCCTGCTTTCCAATCCTAAGAACCCTCAACTCAAGGGTGAGGTACTCTTAGACTATTATTCTCTCGTTTGGGAGTGGATTGGTTGATTGATCGGACCTATTGGGATGACTTAGGACTTTTAATTGTACACGTTTTCTCTTGATTCTGAAACTCAAGGTAGAGATGATTTCCAACTAGGTTTTGATAGGATCTTCCTTGCTTTGATATGATAATTTTTCGTTTAGGTGGATGACAAATACCAATACTCGTGTGAAAACAAGGACTTAAAGGTCCATGGATGGATAAGCACCAATCCGCCCATCGGGTTTTGGCAGATTACACCCAGTGATGAATTTCGATCGGGTGGGCCTCATAAGCAAAGTCTAACATCCCATGTGGGACCTACAACCCTGGCTGTAGGTGATCTAACAACATTAATCATCTTTAGTTTTTTGTTGTCTTGCACATTGATGTATCATAAATTCTGGAGATTTCATGTGACAATTAGGATTTCTGGTGATTCTCATCTTTCCTTTCAACTTGCTCTTTCAGATTTTTCTTAGTGCTCATTATGCGGGACAAGATTTGGTGCCAAAATTTAGAGCTGGTGAGCCATGGAAGAAGGTTTTTGGCCCTGTTTTTCTTTATCTTAATTCCACTTCAGCTGGAGATGATCCATTTTGGTTATGGGAGGATGCTAAAATACAGGTATGATGCATTAAGACACTCATCTTTTGTCTTCGTATGGATTACACTTTGACAAGAAATAATAATAATCATATGATTACCAGATGATGGCCGAAGTTCAAAGTTGGCCGTATAGTTTCCCTGCATCTGAGGATTTCCCGAAAGTGGATCAACGGGGTACTGTTAGCGGTAGGCTTCTGGTTTTTGACAGGTAGGTACGCATTTCATTTTCGTAAATTCCCTGTAAGAAATTTTTTAATATTGTGGCAGTGTTTATGTTGATTGGAAAAATGAAATGCATTGGCCTTTGTTGGTTGCTTTACAATTCATATACTTTCAAACAGAAACAGTGAGGAACCAGTAGTTTCTGTTAACTTATTAGATTTTTACAAAGAGGACATATTTGTTACATGAAAATTACAACTCCTTTCAACTTCAGGATGTAATATAGATGTTGATAAATTATCAATATCCAAAATATATAGCAGATAACCATACATAGAAAAGCAAAGTTTTGTCCAATAATCAAAATTTGTTGTTTTTTATTCATGAATTTATTTCCTATTTTATTTTTATGGTTTCTGAGAAAATCTAAAATTAAAAATGACACTTCTGTAAAGTGGAAATGAAATTCAAGAAACCATACTCCTATATTTGTAAGACTTCCACAAAGAAAATGAAAGGAGTTTTCCAACTTTTCAAATTTGAGATGAAAAAAGATAAACAAGAAACAAAATATTATCAATTAGAACCTCGATCTGTTTAATTTTCTGGTTTAGTTTACGTGCTTGGACCTTTGTTATTAAAATGACTAGGTATATGAGTCAAGACCATCTTCCAGCCAGTGGAGCCTTTGTCGGATTGTCTGCACCAGGAGAAGTTGGGTCATGGCAGAGAGAATGCAAGGTGATTAACTTTATTTCCTCTGGTTTCAATTCCATTACAAATATGCTAGTGTAACAAAGATTATCTATTGTTGTCTACCTCGGCCAAATGAAGTTCAAACTTGTACTTCTACAATTATTCGAGCATATCATTTTTTCCTTACTAGGATGACAGGCATACCACACCAATGAAATGATGGACCCTTGTTCTATGAAGTCATTAAGAAAACTGTTCCTTCTTCACACGTGTGACAAATTTCTTCGTTGAGCTTGATATCTATTGTCTTTAAAATTCAAGATAGCAAAGCTTATCAAAAAGAGATACTTCAATTCACATGTATTATATTTCTAGGATTGGAATGAACAACTTTTTAAATTTCCAAACTTTTCTCATACCTATAAAAGATGTGTTAGAGCCCTGGAAGGATAGACATCCTCTTATTGACTTTAAGGGAAAGGATACAGAGTTCAAAGATGTTTGAGCTTTGAAGGTTCACGTCTCTCCAATGAACTAGAAAAGACTTCTAATTTCCACTGCAAATTCCCTACCCGTTTCTCTTCCTTGTACATATAATTCCCCAGCCCTTACGTCAGTTAACTAACTTCACATTCTTTTGCTGACTTCAGTATAACTAACAACTTATAGCAAAAGACTATCCTGCCCTCCTTTCTGAGACCACCCGAACTCTCGATGATCTAACCTCCATAGTTTCACGATTCTTCCAATACAGCCAAGAACCAGTCTCATGTTTCTATCAAGATGGTATTGAAGAAATTAGAAAAGTAATATGATGAAAGAATAAATACAAAACTCCTTTTGCTTGAGACTTTCATCAGTGTCTATGAAGTCCTTGAAAAGAAATGGAAGATAATGAATGCCTAAGCTTTTAGAACTACAATTTCTCACATGTGGGGTCTACTAACTCTTCGAAACTTGAGGCTTTTAACTACATTTTACTATAAGAACCTTTCGCTAATAGTTTCTAAAAGCTTAAGTCACATGTATGCTTTAAAAGAATTTATCCTCGTTGTCAGGGATATCAATTCTGGACTCGAGCAGATGATCGTGGTTATTTTACCATCAACCACATACACACTGGTGACTACAATCTATATGCATGGGTCCCTGGCTTCATTGGAGATTACCGTTGCGATGCCCTCATCAACGTAAACCAAGGTACAGTTTCCTGTACCTGTAAATTGCCTCAAGTCCACAATTTCTCTTGTACACGGTTATGGTATTCCAAATTGAAATGTAGGATCTTGCATTGAAACGGGCGATCTTGTTTATGAACCTCCAAGAGATGGGCCCACACTATGGGAAATAGGCATTCCCGATCGCTCCGCATCTGAGTTCTATGTTCCTGATCCTAATCCAATGCATATTAATAAGCTCTTTGTAAATCATCCTGACAGGTAGCCCTTTTTCTATAGTCTTATGAGTTAGATGAACTTTGATATTATGGTCCATTTTTACAATCTCTTTCACTATGTTTCTAAATAGAAATTTGAGTTCTGTTACTGAGGATTGCAGTGTGTCTTAATATACCTTTGTAGAATGAAAATGATTTCTGTAGAACATGAGGGATCCAAGTCCAACTAAAGTTGAAATCTCTTCCATTTAACATTTATACTCTGCAGGTTCCGGCAGTACGGGTTGTGGGACAGATATGCGGAATTATACCCGGACGAGGATTTAGTATACACGGTTGGCACAAGTGATTATAGTAAAGATTGGTTCTTTGCTCAGGTCCCTAGGTAACATTTCTTTTGCTATTCACTGTCCATAACCAAGAACAACACCGACAATCATCGTCTCGTCGTCATCCCCACAATTTCTCCTCTTTATTAATGGCTATGATTGTTGTATGATCTTTGACATCTCCTGTATTTGTCATCAAACTATTCCATATATATTGCAGGAAGACAAACAGTAATTCACATGAAGGAACTACTTGGCAAATCAAATTCAAACTTGACAGAGTGGATCACAAGAGTATGTATACGCTACTAGTCGCAATCGCATCTGCAACTCTTGCTGAATTGCAGGTAAGACACGATGTCATTGGCAATAACTGAAGTGCTTAACATTTACTAAAACATGTTCTCTCTCTAGGTCCGGGTTAACGATCCGAGATCGAGGCGACCTTTGTTCACGAGCGGGTTGATAGGGAGAGACAATTCAATTGCAAGACATGGAATTCATGGAATTTATTGGCTTTACAGCGTGGGCATAGCAGGCTCTCATCTTGTTGAAGGAGACAACACAATTTTCTTCACTCAGCCACGCTGCACCAGCCCTTTCCAGGGTATCATGTATGATTATATCCGTCTGGAAGGTCCTCCAACGAATCTTTACTAAACTATTTTATATTGCACTTCTTTTAGCTAAAGTTAAACTCCCTATTGCCTATATATCTATCTTCAATATTTTGTATATGGAGTTATTTTTGGGTATCTCTCTAGGCTTGTATGGTTTTGAAATCGATTTTCTGATTTTTTTTTTTTGGGAGATGTGATAGTTTTTAATGTCTATAGGTATTCACAATCACAAACATATGGATTTTTTTTTTTTTTCCTTAAAGCATAGTAACTTTTAGATGTTC

mRNA sequence

ATGTCGTCCAGGGGAGTGCGATTGCAGATACGAGATCGTCATGTGGTGGTGGATAATGGAATACTGCAACTAACTTTATCAAAACCAGATGGAATCATCACTGGCATACGATATAATGGTGTTGACAATCTGCTTGAAGTTCTCAATCCTGAATCTAACAGAGGGTATTGGGATCTCGTTTGGAATTCTCCAGGAAGCAAGGGAATATTTGACGTGATTAAAGGATCAAGTTTTAGGGTTATAGTGGAAAATGAGGAACAGGTTGAGCTTTCTTTTTTGAGGATGTGGGATCCCTCCCTCGAGGGAAAATTTGTTCCTTTGAATATCGACAAAAGGTTCATAGTGCTACGTGGTTCCTCTGGTTTCTACTCTTATGCTATTTATGAGCACTTGAAGGATTGGCCTGATTTTGATATTGGTGAAACCAGGATCACTTTTAAACTCAGAAAAGACAAGTTTCAGTACATGGCTGTAGCTGACAACAGGCGGAGATTGATGCCCCTGCCCGATGATCGGTTATCTGGAAGTTGTCAAACTTTGGCCTACCCGGAAGCTGTCCTGCTTTCCAATCCTAAGAACCCTCAACTCAAGGGTGAGGTGGATGACAAATACCAATACTCGTGTGAAAACAAGGACTTAAAGGTCCATGGATGGATAAGCACCAATCCGCCCATCGGGTTTTGGCAGATTACACCCAGTGATGAATTTCGATCGGGTGGGCCTCATAAGCAAAGTCTAACATCCCATGTGGGACCTACAACCCTGGCTATTTTTCTTAGTGCTCATTATGCGGGACAAGATTTGGTGCCAAAATTTAGAGCTGGTGAGCCATGGAAGAAGGTTTTTGGCCCTGTTTTTCTTTATCTTAATTCCACTTCAGCTGGAGATGATCCATTTTGGTTATGGGAGGATGCTAAAATACAGATGATGGCCGAAGTTCAAAGTTGGCCGTATAGTTTCCCTGCATCTGAGGATTTCCCGAAAGTGGATCAACGGGGTACTGTTAGCGGTAGGCTTCTGGTTTTTGACAGGTATATGAGTCAAGACCATCTTCCAGCCAGTGGAGCCTTTGTCGGATTGTCTGCACCAGGAGAAGTTGGGTCATGGCAGAGAGAATGCAAGGGATATCAATTCTGGACTCGAGCAGATGATCGTGGTTATTTTACCATCAACCACATACACACTGGTGACTACAATCTATATGCATGGGTCCCTGGCTTCATTGGAGATTACCGTTGCGATGCCCTCATCAACGTAAACCAAGGATCTTGCATTGAAACGGGCGATCTTGTTTATGAACCTCCAAGAGATGGGCCCACACTATGGGAAATAGGCATTCCCGATCGCTCCGCATCTGAGTTCTATGTTCCTGATCCTAATCCAATGCATATTAATAAGCTCTTTGTAAATCATCCTGACAGGTTCCGGCAGTACGGGTTGTGGGACAGATATGCGGAATTATACCCGGACGAGGATTTAGTATACACGGTTGGCACAAGTGATTATAGTAAAGATTGGTTCTTTGCTCAGGTCCCTAGGAAGACAAACAGTAATTCACATGAAGGAACTACTTGGCAAATCAAATTCAAACTTGACAGAGTGGATCACAAGAGTATGTATACGCTACTAGTCGCAATCGCATCTGCAACTCTTGCTGAATTGCAGGTCCGGGTTAACGATCCGAGATCGAGGCGACCTTTGTTCACGAGCGGGTTGATAGGGAGAGACAATTCAATTGCAAGACATGGAATTCATGGAATTTATTGGCTTTACAGCGTGGGCATAGCAGGCTCTCATCTTGTTGAAGGAGACAACACAATTTTCTTCACTCAGCCACGCTGCACCAGCCCTTTCCAGGGTATCATGTATGATTATATCCGTCTGGAAGGTCCTCCAACGAATCTTTACTAA

Coding sequence (CDS)

ATGTCGTCCAGGGGAGTGCGATTGCAGATACGAGATCGTCATGTGGTGGTGGATAATGGAATACTGCAACTAACTTTATCAAAACCAGATGGAATCATCACTGGCATACGATATAATGGTGTTGACAATCTGCTTGAAGTTCTCAATCCTGAATCTAACAGAGGGTATTGGGATCTCGTTTGGAATTCTCCAGGAAGCAAGGGAATATTTGACGTGATTAAAGGATCAAGTTTTAGGGTTATAGTGGAAAATGAGGAACAGGTTGAGCTTTCTTTTTTGAGGATGTGGGATCCCTCCCTCGAGGGAAAATTTGTTCCTTTGAATATCGACAAAAGGTTCATAGTGCTACGTGGTTCCTCTGGTTTCTACTCTTATGCTATTTATGAGCACTTGAAGGATTGGCCTGATTTTGATATTGGTGAAACCAGGATCACTTTTAAACTCAGAAAAGACAAGTTTCAGTACATGGCTGTAGCTGACAACAGGCGGAGATTGATGCCCCTGCCCGATGATCGGTTATCTGGAAGTTGTCAAACTTTGGCCTACCCGGAAGCTGTCCTGCTTTCCAATCCTAAGAACCCTCAACTCAAGGGTGAGGTGGATGACAAATACCAATACTCGTGTGAAAACAAGGACTTAAAGGTCCATGGATGGATAAGCACCAATCCGCCCATCGGGTTTTGGCAGATTACACCCAGTGATGAATTTCGATCGGGTGGGCCTCATAAGCAAAGTCTAACATCCCATGTGGGACCTACAACCCTGGCTATTTTTCTTAGTGCTCATTATGCGGGACAAGATTTGGTGCCAAAATTTAGAGCTGGTGAGCCATGGAAGAAGGTTTTTGGCCCTGTTTTTCTTTATCTTAATTCCACTTCAGCTGGAGATGATCCATTTTGGTTATGGGAGGATGCTAAAATACAGATGATGGCCGAAGTTCAAAGTTGGCCGTATAGTTTCCCTGCATCTGAGGATTTCCCGAAAGTGGATCAACGGGGTACTGTTAGCGGTAGGCTTCTGGTTTTTGACAGGTATATGAGTCAAGACCATCTTCCAGCCAGTGGAGCCTTTGTCGGATTGTCTGCACCAGGAGAAGTTGGGTCATGGCAGAGAGAATGCAAGGGATATCAATTCTGGACTCGAGCAGATGATCGTGGTTATTTTACCATCAACCACATACACACTGGTGACTACAATCTATATGCATGGGTCCCTGGCTTCATTGGAGATTACCGTTGCGATGCCCTCATCAACGTAAACCAAGGATCTTGCATTGAAACGGGCGATCTTGTTTATGAACCTCCAAGAGATGGGCCCACACTATGGGAAATAGGCATTCCCGATCGCTCCGCATCTGAGTTCTATGTTCCTGATCCTAATCCAATGCATATTAATAAGCTCTTTGTAAATCATCCTGACAGGTTCCGGCAGTACGGGTTGTGGGACAGATATGCGGAATTATACCCGGACGAGGATTTAGTATACACGGTTGGCACAAGTGATTATAGTAAAGATTGGTTCTTTGCTCAGGTCCCTAGGAAGACAAACAGTAATTCACATGAAGGAACTACTTGGCAAATCAAATTCAAACTTGACAGAGTGGATCACAAGAGTATGTATACGCTACTAGTCGCAATCGCATCTGCAACTCTTGCTGAATTGCAGGTCCGGGTTAACGATCCGAGATCGAGGCGACCTTTGTTCACGAGCGGGTTGATAGGGAGAGACAATTCAATTGCAAGACATGGAATTCATGGAATTTATTGGCTTTACAGCGTGGGCATAGCAGGCTCTCATCTTGTTGAAGGAGACAACACAATTTTCTTCACTCAGCCACGCTGCACCAGCCCTTTCCAGGGTATCATGTATGATTATATCCGTCTGGAAGGTCCTCCAACGAATCTTTACTAA

Protein sequence

MSSRGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLVWNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSSGFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRRRLMPLPDDRLSGSCQTLAYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPPIGFWQITPSDEFRSGGPHKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFWLWEDAKIQMMAEVQSWPYSFPASEDFPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDYRCDALINVNQGSCIETGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMYTLLVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGSHLVEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPTNLY
Homology
BLAST of Spg017051 vs. NCBI nr
Match: XP_038897922.1 (probable rhamnogalacturonate lyase B [Benincasa hispida])

HSP 1 Score: 1280.4 bits (3312), Expect = 0.0e+00
Identity = 593/634 (93.53%), Postives = 610/634 (96.21%), Query Frame = 0

Query: 1   MSSRGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSSRGVRLQIRD HVVV+NGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV
Sbjct: 1   MSSRGVRLQIRDHHVVVENGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGSKGIFDVIKGSSFRVIVENEEQVELSF+RMWDPSLEGK VPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFMRMWDPSLEGKLVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRRRLMPLPDDRLSGSCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNR+++MPLPDDRLSG CQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQKVMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPPIGFWQITPSDEFRSGG 240
           AYPEAVLL+NPKNP+LKGEVDDKYQYSCENKDLKVHGWI TNPP GFW ITPSDEFRSGG
Sbjct: 181 AYPEAVLLTNPKNPELKGEVDDKYQYSCENKDLKVHGWICTNPPTGFWHITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           P KQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVF YLNSTS GDDPFW
Sbjct: 241 PLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFFYLNSTSVGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDFPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEV+SWPYSFPASEDFPKVDQRG V GRLLV DRY+SQD+LPASGAFVG+
Sbjct: 301 LWEDAKIQMMAEVESWPYSFPASEDFPKVDQRGVVRGRLLVLDRYVSQDYLPASGAFVGI 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDYRCDALINVN 420
           SAPGEVGSWQRECKGYQFWTRADD GYFTINH HTGDYNLY+WVPGFIGDYRCDALINVN
Sbjct: 361 SAPGEVGSWQRECKGYQFWTRADDGGYFTINHAHTGDYNLYSWVPGFIGDYRCDALINVN 420

Query: 421 QGSCIETGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGSCIE GDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNP HINKLFVNHPDRFRQYGL
Sbjct: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPTHINKLFVNHPDRFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMY 540
           WDRYAELYP+EDLVYTVGTSDYSKDWFFAQVPRKT SNSHEGTTWQIKF L+RV  KSMY
Sbjct: 481 WDRYAELYPNEDLVYTVGTSDYSKDWFFAQVPRKTKSNSHEGTTWQIKFYLNRVARKSMY 540

Query: 541 TLLVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGSHL 600
           TL VAIASATLAELQVRVNDPRS+RPLFTSGLIGRDNSIARHGIHGIYWLYSVG+ G HL
Sbjct: 541 TLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWLYSVGVGGCHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPTN 635
           VEG+NTIF TQPRCTSPFQGIMYDYIRLEGPPTN
Sbjct: 601 VEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPTN 634

BLAST of Spg017051 vs. NCBI nr
Match: XP_004136250.1 (probable rhamnogalacturonate lyase B isoform X1 [Cucumis sativus] >KGN60276.1 hypothetical protein Csa_001404 [Cucumis sativus])

HSP 1 Score: 1270.8 bits (3287), Expect = 0.0e+00
Identity = 588/633 (92.89%), Postives = 608/633 (96.05%), Query Frame = 0

Query: 1   MSSRGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSSR VRLQIRD HVVVDNGILQLTLSKPDGIITG+RYNGVDNLLEVLNPESNRGYWDLV
Sbjct: 1   MSSRAVRLQIRDHHVVVDNGILQLTLSKPDGIITGVRYNGVDNLLEVLNPESNRGYWDLV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGSKG+FDVIKGS FRVIVENEEQVELSF+RMWDPSLEGKFVPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSKGVFDVIKGSRFRVIVENEEQVELSFVRMWDPSLEGKFVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRRRLMPLPDDRLSGSCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNR+R+MPLPDDRLSG CQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRVMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPPIGFWQITPSDEFRSGG 240
           AYPEAVLL+NPKNP LKGEVDDKYQYSCEN +LKVHGWISTNPP GFWQITPSDEFRSGG
Sbjct: 181 AYPEAVLLTNPKNPALKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           P KQSLTSHVGPTTLAIFLS HYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTS GDDPFW
Sbjct: 241 PLKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDFPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEV SWPYSFPASEDFPK DQRG VSGRLLVFDRY+ QD+LPA GAFVGL
Sbjct: 301 LWEDAKIQMMAEVDSWPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDYRCDALINVN 420
           SAPGE GSWQRECKGYQFWTRADD GYFTINH+HTG+YNLYAWVPGFIGDYRCDALINV+
Sbjct: 361 SAPGEAGSWQRECKGYQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVS 420

Query: 421 QGSCIETGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGSCIE GDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNP+HINKLFV+HPDRFRQYGL
Sbjct: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMY 540
           W+RYAELYP+EDLVYTVGTSDY KDWFFA VPRKTN NSHEGTTWQIKFKLDRVDHKSMY
Sbjct: 481 WNRYAELYPNEDLVYTVGTSDYRKDWFFAHVPRKTN-NSHEGTTWQIKFKLDRVDHKSMY 540

Query: 541 TLLVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGSHL 600
           TL VAIASATLAELQVRVNDPRS+RPLFTSGLIGRDNSIARHGIHGIYW+Y VG+AG HL
Sbjct: 541 TLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPT 634
           VEG+NTIF TQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 601 VEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 632

BLAST of Spg017051 vs. NCBI nr
Match: XP_022980958.1 (probable rhamnogalacturonate lyase B [Cucurbita maxima])

HSP 1 Score: 1266.5 bits (3276), Expect = 0.0e+00
Identity = 584/634 (92.11%), Postives = 608/634 (95.90%), Query Frame = 0

Query: 1   MSSRGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSSRGVRLQIRD HVVVDNGILQLTLSKPDGIITGI YNGVDNLLEVLNPESNRGYWD+V
Sbjct: 1   MSSRGVRLQIRDHHVVVDNGILQLTLSKPDGIITGIHYNGVDNLLEVLNPESNRGYWDIV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGS+GIFDVIKGSSFRVIVENEEQVELSF RMWDPSLEGK VPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSQGIFDVIKGSSFRVIVENEEQVELSFTRMWDPSLEGKVVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRRRLMPLPDDRLSGSCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNR+RLMPLPDDRLSG CQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPPIGFWQITPSDEFRSGG 240
            +PEAVLL+NPKNP+ + EVDDKYQYSCENKDLKVHGWIS NPPIGFWQITPSDEFRSGG
Sbjct: 181 GFPEAVLLTNPKNPEHRDEVDDKYQYSCENKDLKVHGWISNNPPIGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           PHKQSLTSHVGPTTLAIFLS HYAGQDLVPKFR GEPWKKVFGPVFLYLNST AGDDPFW
Sbjct: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRNGEPWKKVFGPVFLYLNSTKAGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDFPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEVQSWPYSFPAS++FPK+DQRGT+SGRLLVFDRYMS+D+LPASGAFVGL
Sbjct: 301 LWEDAKIQMMAEVQSWPYSFPASDNFPKLDQRGTISGRLLVFDRYMSRDYLPASGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDYRCDALINVN 420
           SAPGEVGSWQRECKGYQFWTRADDRGYF INH+H G YNLYAWVPGFIGDYRCDALIN++
Sbjct: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFIINHVHIGQYNLYAWVPGFIGDYRCDALINIS 420

Query: 421 QGSCIETGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGS IE G+LVYEPPRDGPTLWEIGIPDRSASEFY+PDPNPMHINKLFVNHP+RFRQYGL
Sbjct: 421 QGSSIELGNLVYEPPRDGPTLWEIGIPDRSASEFYIPDPNPMHINKLFVNHPERFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMY 540
           W+RYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTN+NSH+GTTW+I FKLDRVD KS Y
Sbjct: 481 WERYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNNNSHQGTTWKIAFKLDRVDRKSRY 540

Query: 541 TLLVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGSHL 600
            L VAIASATLAELQVRVNDP S+RPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAG HL
Sbjct: 541 MLRVAIASATLAELQVRVNDPSSKRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGLHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPTN 635
           VEGDN IFFTQPRCTSPFQGIMYDYIRLEGP TN
Sbjct: 601 VEGDNAIFFTQPRCTSPFQGIMYDYIRLEGPSTN 634

BLAST of Spg017051 vs. NCBI nr
Match: XP_022940913.1 (probable rhamnogalacturonate lyase B isoform X1 [Cucurbita moschata])

HSP 1 Score: 1266.5 bits (3276), Expect = 0.0e+00
Identity = 583/634 (91.96%), Postives = 607/634 (95.74%), Query Frame = 0

Query: 1   MSSRGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSSRGVRLQIRD HVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWD+V
Sbjct: 1   MSSRGVRLQIRDHHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDIV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGS+GIFDVIKGSSFRVIVENEEQVELSF RMWDPSLEGK VPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSQGIFDVIKGSSFRVIVENEEQVELSFTRMWDPSLEGKVVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRRRLMPLPDDRLSGSCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNR+RLMPLPDDRLSG CQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPPIGFWQITPSDEFRSGG 240
            +PEAVLL+NPKNP+ + EVDDKYQYSC+NKDLKVHGWISTNPPIGFWQITPSDEFRSGG
Sbjct: 181 GFPEAVLLTNPKNPEHRDEVDDKYQYSCQNKDLKVHGWISTNPPIGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           PHKQSLTSHVGPTTLAIFLS HYAGQDLVPKFR GEPWKKVFGPVFLYLNST AGDDPFW
Sbjct: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRNGEPWKKVFGPVFLYLNSTKAGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDFPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEVQSWPYSFPAS+DFPK+DQRGT+SGRLLVFDRYMS+D+LPA GAFVGL
Sbjct: 301 LWEDAKIQMMAEVQSWPYSFPASDDFPKLDQRGTISGRLLVFDRYMSRDYLPAGGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDYRCDALINVN 420
           SAPGEVGSWQRECKGYQFWTRADDRGYF INH+H G YNLYAWVPGFIGDYRCDALIN++
Sbjct: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFIINHVHIGQYNLYAWVPGFIGDYRCDALINIS 420

Query: 421 QGSCIETGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGS IE GDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNP+HINKLFVNHP+RFRQYGL
Sbjct: 421 QGSSIELGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPLHINKLFVNHPERFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMY 540
           W+RYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTN NSH+GTTW+I FKLDRVD K+ Y
Sbjct: 481 WERYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNDNSHQGTTWKIAFKLDRVDRKNRY 540

Query: 541 TLLVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGSHL 600
            L VAIASATLAELQ+RVNDP S+RPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAG HL
Sbjct: 541 MLRVAIASATLAELQIRVNDPSSKRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGLHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPTN 635
           VEGDN IFFTQPRCTSPFQGIMYDYIRLE P TN
Sbjct: 601 VEGDNAIFFTQPRCTSPFQGIMYDYIRLEAPSTN 634

BLAST of Spg017051 vs. NCBI nr
Match: XP_008466137.1 (PREDICTED: probable rhamnogalacturonate lyase B isoform X1 [Cucumis melo] >KAA0038653.1 putative rhamnogalacturonate lyase B isoform X1 [Cucumis melo var. makuwa] >TYK31256.1 putative rhamnogalacturonate lyase B isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1263.1 bits (3267), Expect = 0.0e+00
Identity = 586/633 (92.58%), Postives = 606/633 (95.73%), Query Frame = 0

Query: 1   MSSRGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSS+ VRLQIRD HVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV
Sbjct: 1   MSSKAVRLQIRDHHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGSKGIFDVIKGS F VIVENEEQVELSF+RMWDPSLEGKFVPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSKGIFDVIKGSRFSVIVENEEQVELSFVRMWDPSLEGKFVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRRRLMPLPDDRLSGSCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNR+R+MPLPDDRLSG CQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRVMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPPIGFWQITPSDEFRSGG 240
           AYPEAVLL++PKN  LKGEVDDKYQYSCEN +LKVHGWISTNPP GFWQITPSDEFRSGG
Sbjct: 181 AYPEAVLLTDPKNSALKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           P KQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTS GDDPFW
Sbjct: 241 PLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDFPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEV+SWPYSFPASEDF K DQRG VSGRLLVFDRY+ QD+LP SGAFVGL
Sbjct: 301 LWEDAKIQMMAEVESWPYSFPASEDFSKKDQRGMVSGRLLVFDRYVDQDYLPGSGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDYRCDALINVN 420
           SAPGEVGSWQRECKGYQFWTRADD GYFTINH+ TGDYNLYAWVPGFIGDYRCDAL+NV+
Sbjct: 361 SAPGEVGSWQRECKGYQFWTRADDGGYFTINHVLTGDYNLYAWVPGFIGDYRCDALVNVS 420

Query: 421 QGSCIETGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGSCIE GDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNP++INKLFVNHPDRFRQYGL
Sbjct: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPIYINKLFVNHPDRFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMY 540
           WDRYAELYPDEDLVYTVGTSDY KDWFFA VPRKTNSNSHEGTTWQIKF+LDRVD KSMY
Sbjct: 481 WDRYAELYPDEDLVYTVGTSDYRKDWFFAHVPRKTNSNSHEGTTWQIKFQLDRVDRKSMY 540

Query: 541 TLLVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGSHL 600
            L VAIASATLAELQVRVNDPRS+RPLFTSGLIGRDNSIARHGIHGIYW+YSV +AG HL
Sbjct: 541 MLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYSVSVAGCHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPT 634
           VEG+NTIF TQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 601 VEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 633

BLAST of Spg017051 vs. ExPASy Swiss-Prot
Match: Q5AZ85 (Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglB PE=2 SV=2)

HSP 1 Score: 102.8 bits (255), Expect = 1.4e-20
Identity = 148/672 (22.02%), Postives = 246/672 (36.61%), Query Frame = 0

Query: 12  DRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLVWNSPGSKGIFD 71
           D  +V++N  L+ T  K  G I  +  +G D L           Y D      G      
Sbjct: 26  DTSIVLENDRLRATFDKGRGSIIDLYLDGQDFLGPQSGSTGIGPYLDCYCTPSG------ 85

Query: 72  VIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNID-KRFIVLR-GSSGFYSYAIYE 131
                S   + E  +  + +  +     L   + P   + +++  LR G +GF+ ++   
Sbjct: 86  FYTAGSTNPVTELVQGTDSTGTKYAGIILNDTYTPTGQEFQQYWFLRDGETGFHMFSRLA 145

Query: 132 HLKDWPDF--DIGETRITFKLRKDKFQYMAVADNRRRLMPLPDDRLSGSCQTLAYPEAVL 191
           +  +   F  ++ E R  F+   D + ++  +D   +  PLP D      Q +       
Sbjct: 146 YYNETTPFLRNLQELRTLFRPNTDLWTHLTSSD--LQTAPLPSDEAIAE-QIVVQDATWR 205

Query: 192 LSNPKNP---QLKGEVDDKYQYSCENKDLKVHGW-----ISTNPPIGFWQITPSDEFRSG 251
           L+N  +    Q   E   KY +S   +D  VHG       S     G W +  + +   G
Sbjct: 206 LNNTPDDAYYQQFSEYFTKYTFSNHWRDNDVHGLYADGSTSDGTTYGAWLVMNTKDTYYG 265

Query: 252 GPHKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPF 311
           GP    LT  V        +S H+   +  P    G  + + FGP F   N   +     
Sbjct: 266 GPLHSDLT--VDGIIYNYIVSNHHG--EGTPNITNG--FDRTFGPQFYLFNGGGSSS--- 325

Query: 312 WLWEDAKIQMMAEVQSWPYSFPAS-----EDFPKVDQRGTVSGRLLVFDRYMSQDHLP-- 371
            L E           SW   F  S       +    QRG+V G +           LP  
Sbjct: 326 -LEELRDEARSLASPSWNADFYDSIAKHVIGYVPSSQRGSVKGTI----------KLPKN 385

Query: 372 ASGAFVGLSAPGEVGSWQREC-KGYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDY 431
           A      L+  G            +Q+W   D  G F+I+ +  G Y L  +  G  GD+
Sbjct: 386 AKSPIAVLTVDGHYFQDNSAVPSSHQYWADIDKNGRFSIDRVVAGKYRLTVYADGIFGDF 445

Query: 432 RCDAL-INVNQGSCIETGDLVYEPPRDGPTLWEIGIPDRSASEF-YVPDPNPMHINKLFV 491
             D + +   + + I+     ++P   G  +W +G PD+S+ EF +    +P H      
Sbjct: 446 TRDGIVVKARKSTSIKE---TWKPESAGTEIWRLGTPDKSSGEFRHGAARDPTH-----P 505

Query: 492 NHPDRFRQY-GLWDRYAELYPDEDLVYTVGTSDYSKDW------FFAQVPRKTNSNSHEG 551
            HP  +  Y G +D  ++     D  Y +G SD + D+       F   P    + S+  
Sbjct: 506 RHPPEYLIYWGAYDWQSDFPGGID--YMIGESDPATDFNTVHWAVFGPTPDNPVAESNTT 565

Query: 552 TTWQIKFKLD--RVDHKSMYTLLVAIASATLAELQVRV---NDPRSRRPLFT-------- 611
             W+I+F L   ++  +   TL + +A A  A     V   ++P +  PL +        
Sbjct: 566 HDWRIRFDLSAKQLHARKTATLTIQLAGAKAASGNTDVYNASEPYANLPLRSYINEQEEP 625

Query: 612 -SGLIGRDNS---IARHGIHGIYWLYSVGIAGSHLVEGDNTIFFTQPRCTSPFQG----- 630
            + +IG D S   I R  +             S L EG N +  + P   + ++      
Sbjct: 626 LTMVIGYDQSSSCIVRSAVSCYQVREKWEFPASWLKEGSNLLRLSLPTNGTNYESAVLPT 658

BLAST of Spg017051 vs. ExPASy Swiss-Prot
Match: A5ABH4 (Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rglB PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 2.3e-18
Identity = 99/376 (26.33%), Postives = 150/376 (39.89%), Query Frame = 0

Query: 203 KYQYSCENKDLKVHGW-----ISTNPPIGFWQITPSDEFRSGGPHKQSLTSHVGPTTLAI 262
           KY +S   +D  VHG       S     G W +  + +   GGP    LT  V       
Sbjct: 216 KYTFSNAWRDNSVHGMYADGSTSNGSTFGAWLVMNTKDTYYGGPLHSDLT--VDGIVYNY 275

Query: 263 FLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW-LWEDAKIQMMAEVQSW 322
            +S H+   +  P    G  + + FGP + + N           L  DA  + +A+  SW
Sbjct: 276 LVSNHHG--EGTPNITYG--FDRTFGPQYYHFNGGKGSTASLQELKSDA--ETLAD-PSW 335

Query: 323 PYSFPAS-----EDFPKVDQRGTVSGRLLVFDRYMSQDHLP--ASGAFVGLSAPGEVGSW 382
              F  S       +    QRG+V G++           LP  A+     L+  G+    
Sbjct: 336 NVDFYDSIAKHVVGYTPSSQRGSVQGKI----------KLPKGATRPIAVLTVDGQYFQD 395

Query: 383 QR-ECKGYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDYRCDALINVNQGSCIETG 442
                  YQ+W   DD G+F+++H+  G Y L  +  G  GD+  D  + V  G    T 
Sbjct: 396 NSVNSSSYQYWAEIDDSGHFSVDHVKEGPYRLTVYADGIFGDFVRDG-VQVKAGK-KTTI 455

Query: 443 DLVYEPPRDGPTLWEIGIPDRSASEF---YVPDP-NPMHINKLFVNHPDRFRQYGLWDRY 502
              +E    G  +W +G PD+S+ EF      DP +P+H        P+    +G +D  
Sbjct: 456 QETWEAESAGTEIWRLGTPDKSSGEFRHGVARDPTHPLH-------PPEYLIYWGAYDWQ 515

Query: 503 AELYPDEDLVYTVGTSDYSKD-----W-FFAQVPRKTNSNSHEGTTWQIKFKL--DRVDH 553
           ++ +PD  + YT+GTSD + D     W  F   P            W I F L  D +  
Sbjct: 516 SD-FPD-GINYTIGTSDPATDLNTVHWSVFGPTPNDPRVEYDTTHDWTINFPLSEDDLAE 561

BLAST of Spg017051 vs. ExPASy TrEMBL
Match: A0A0A0LJQ9 (Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_3G892770 PE=3 SV=1)

HSP 1 Score: 1270.8 bits (3287), Expect = 0.0e+00
Identity = 588/633 (92.89%), Postives = 608/633 (96.05%), Query Frame = 0

Query: 1   MSSRGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSSR VRLQIRD HVVVDNGILQLTLSKPDGIITG+RYNGVDNLLEVLNPESNRGYWDLV
Sbjct: 1   MSSRAVRLQIRDHHVVVDNGILQLTLSKPDGIITGVRYNGVDNLLEVLNPESNRGYWDLV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGSKG+FDVIKGS FRVIVENEEQVELSF+RMWDPSLEGKFVPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSKGVFDVIKGSRFRVIVENEEQVELSFVRMWDPSLEGKFVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRRRLMPLPDDRLSGSCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNR+R+MPLPDDRLSG CQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRVMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPPIGFWQITPSDEFRSGG 240
           AYPEAVLL+NPKNP LKGEVDDKYQYSCEN +LKVHGWISTNPP GFWQITPSDEFRSGG
Sbjct: 181 AYPEAVLLTNPKNPALKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           P KQSLTSHVGPTTLAIFLS HYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTS GDDPFW
Sbjct: 241 PLKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDFPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEV SWPYSFPASEDFPK DQRG VSGRLLVFDRY+ QD+LPA GAFVGL
Sbjct: 301 LWEDAKIQMMAEVDSWPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDYRCDALINVN 420
           SAPGE GSWQRECKGYQFWTRADD GYFTINH+HTG+YNLYAWVPGFIGDYRCDALINV+
Sbjct: 361 SAPGEAGSWQRECKGYQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVS 420

Query: 421 QGSCIETGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGSCIE GDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNP+HINKLFV+HPDRFRQYGL
Sbjct: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMY 540
           W+RYAELYP+EDLVYTVGTSDY KDWFFA VPRKTN NSHEGTTWQIKFKLDRVDHKSMY
Sbjct: 481 WNRYAELYPNEDLVYTVGTSDYRKDWFFAHVPRKTN-NSHEGTTWQIKFKLDRVDHKSMY 540

Query: 541 TLLVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGSHL 600
           TL VAIASATLAELQVRVNDPRS+RPLFTSGLIGRDNSIARHGIHGIYW+Y VG+AG HL
Sbjct: 541 TLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPT 634
           VEG+NTIF TQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 601 VEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 632

BLAST of Spg017051 vs. ExPASy TrEMBL
Match: A0A6J1J0S6 (Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111480252 PE=3 SV=1)

HSP 1 Score: 1266.5 bits (3276), Expect = 0.0e+00
Identity = 584/634 (92.11%), Postives = 608/634 (95.90%), Query Frame = 0

Query: 1   MSSRGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSSRGVRLQIRD HVVVDNGILQLTLSKPDGIITGI YNGVDNLLEVLNPESNRGYWD+V
Sbjct: 1   MSSRGVRLQIRDHHVVVDNGILQLTLSKPDGIITGIHYNGVDNLLEVLNPESNRGYWDIV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGS+GIFDVIKGSSFRVIVENEEQVELSF RMWDPSLEGK VPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSQGIFDVIKGSSFRVIVENEEQVELSFTRMWDPSLEGKVVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRRRLMPLPDDRLSGSCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNR+RLMPLPDDRLSG CQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPPIGFWQITPSDEFRSGG 240
            +PEAVLL+NPKNP+ + EVDDKYQYSCENKDLKVHGWIS NPPIGFWQITPSDEFRSGG
Sbjct: 181 GFPEAVLLTNPKNPEHRDEVDDKYQYSCENKDLKVHGWISNNPPIGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           PHKQSLTSHVGPTTLAIFLS HYAGQDLVPKFR GEPWKKVFGPVFLYLNST AGDDPFW
Sbjct: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRNGEPWKKVFGPVFLYLNSTKAGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDFPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEVQSWPYSFPAS++FPK+DQRGT+SGRLLVFDRYMS+D+LPASGAFVGL
Sbjct: 301 LWEDAKIQMMAEVQSWPYSFPASDNFPKLDQRGTISGRLLVFDRYMSRDYLPASGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDYRCDALINVN 420
           SAPGEVGSWQRECKGYQFWTRADDRGYF INH+H G YNLYAWVPGFIGDYRCDALIN++
Sbjct: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFIINHVHIGQYNLYAWVPGFIGDYRCDALINIS 420

Query: 421 QGSCIETGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGS IE G+LVYEPPRDGPTLWEIGIPDRSASEFY+PDPNPMHINKLFVNHP+RFRQYGL
Sbjct: 421 QGSSIELGNLVYEPPRDGPTLWEIGIPDRSASEFYIPDPNPMHINKLFVNHPERFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMY 540
           W+RYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTN+NSH+GTTW+I FKLDRVD KS Y
Sbjct: 481 WERYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNNNSHQGTTWKIAFKLDRVDRKSRY 540

Query: 541 TLLVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGSHL 600
            L VAIASATLAELQVRVNDP S+RPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAG HL
Sbjct: 541 MLRVAIASATLAELQVRVNDPSSKRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGLHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPTN 635
           VEGDN IFFTQPRCTSPFQGIMYDYIRLEGP TN
Sbjct: 601 VEGDNAIFFTQPRCTSPFQGIMYDYIRLEGPSTN 634

BLAST of Spg017051 vs. ExPASy TrEMBL
Match: A0A6J1FQM1 (Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111446358 PE=3 SV=1)

HSP 1 Score: 1266.5 bits (3276), Expect = 0.0e+00
Identity = 583/634 (91.96%), Postives = 607/634 (95.74%), Query Frame = 0

Query: 1   MSSRGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSSRGVRLQIRD HVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWD+V
Sbjct: 1   MSSRGVRLQIRDHHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDIV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGS+GIFDVIKGSSFRVIVENEEQVELSF RMWDPSLEGK VPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSQGIFDVIKGSSFRVIVENEEQVELSFTRMWDPSLEGKVVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRRRLMPLPDDRLSGSCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNR+RLMPLPDDRLSG CQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPPIGFWQITPSDEFRSGG 240
            +PEAVLL+NPKNP+ + EVDDKYQYSC+NKDLKVHGWISTNPPIGFWQITPSDEFRSGG
Sbjct: 181 GFPEAVLLTNPKNPEHRDEVDDKYQYSCQNKDLKVHGWISTNPPIGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           PHKQSLTSHVGPTTLAIFLS HYAGQDLVPKFR GEPWKKVFGPVFLYLNST AGDDPFW
Sbjct: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRNGEPWKKVFGPVFLYLNSTKAGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDFPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEVQSWPYSFPAS+DFPK+DQRGT+SGRLLVFDRYMS+D+LPA GAFVGL
Sbjct: 301 LWEDAKIQMMAEVQSWPYSFPASDDFPKLDQRGTISGRLLVFDRYMSRDYLPAGGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDYRCDALINVN 420
           SAPGEVGSWQRECKGYQFWTRADDRGYF INH+H G YNLYAWVPGFIGDYRCDALIN++
Sbjct: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFIINHVHIGQYNLYAWVPGFIGDYRCDALINIS 420

Query: 421 QGSCIETGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGS IE GDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNP+HINKLFVNHP+RFRQYGL
Sbjct: 421 QGSSIELGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPLHINKLFVNHPERFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMY 540
           W+RYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTN NSH+GTTW+I FKLDRVD K+ Y
Sbjct: 481 WERYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNDNSHQGTTWKIAFKLDRVDRKNRY 540

Query: 541 TLLVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGSHL 600
            L VAIASATLAELQ+RVNDP S+RPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAG HL
Sbjct: 541 MLRVAIASATLAELQIRVNDPSSKRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGLHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPTN 635
           VEGDN IFFTQPRCTSPFQGIMYDYIRLE P TN
Sbjct: 601 VEGDNAIFFTQPRCTSPFQGIMYDYIRLEAPSTN 634

BLAST of Spg017051 vs. ExPASy TrEMBL
Match: A0A5D3E6J1 (Rhamnogalacturonan endolyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005450 PE=3 SV=1)

HSP 1 Score: 1263.1 bits (3267), Expect = 0.0e+00
Identity = 586/633 (92.58%), Postives = 606/633 (95.73%), Query Frame = 0

Query: 1   MSSRGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSS+ VRLQIRD HVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV
Sbjct: 1   MSSKAVRLQIRDHHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGSKGIFDVIKGS F VIVENEEQVELSF+RMWDPSLEGKFVPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSKGIFDVIKGSRFSVIVENEEQVELSFVRMWDPSLEGKFVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRRRLMPLPDDRLSGSCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNR+R+MPLPDDRLSG CQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRVMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPPIGFWQITPSDEFRSGG 240
           AYPEAVLL++PKN  LKGEVDDKYQYSCEN +LKVHGWISTNPP GFWQITPSDEFRSGG
Sbjct: 181 AYPEAVLLTDPKNSALKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           P KQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTS GDDPFW
Sbjct: 241 PLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDFPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEV+SWPYSFPASEDF K DQRG VSGRLLVFDRY+ QD+LP SGAFVGL
Sbjct: 301 LWEDAKIQMMAEVESWPYSFPASEDFSKKDQRGMVSGRLLVFDRYVDQDYLPGSGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDYRCDALINVN 420
           SAPGEVGSWQRECKGYQFWTRADD GYFTINH+ TGDYNLYAWVPGFIGDYRCDAL+NV+
Sbjct: 361 SAPGEVGSWQRECKGYQFWTRADDGGYFTINHVLTGDYNLYAWVPGFIGDYRCDALVNVS 420

Query: 421 QGSCIETGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGSCIE GDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNP++INKLFVNHPDRFRQYGL
Sbjct: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPIYINKLFVNHPDRFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMY 540
           WDRYAELYPDEDLVYTVGTSDY KDWFFA VPRKTNSNSHEGTTWQIKF+LDRVD KSMY
Sbjct: 481 WDRYAELYPDEDLVYTVGTSDYRKDWFFAHVPRKTNSNSHEGTTWQIKFQLDRVDRKSMY 540

Query: 541 TLLVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGSHL 600
            L VAIASATLAELQVRVNDPRS+RPLFTSGLIGRDNSIARHGIHGIYW+YSV +AG HL
Sbjct: 541 MLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYSVSVAGCHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPT 634
           VEG+NTIF TQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 601 VEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 633

BLAST of Spg017051 vs. ExPASy TrEMBL
Match: A0A1S3CQJ4 (Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103503644 PE=3 SV=1)

HSP 1 Score: 1263.1 bits (3267), Expect = 0.0e+00
Identity = 586/633 (92.58%), Postives = 606/633 (95.73%), Query Frame = 0

Query: 1   MSSRGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSS+ VRLQIRD HVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV
Sbjct: 1   MSSKAVRLQIRDHHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGSKGIFDVIKGS F VIVENEEQVELSF+RMWDPSLEGKFVPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSKGIFDVIKGSRFSVIVENEEQVELSFVRMWDPSLEGKFVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRRRLMPLPDDRLSGSCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNR+R+MPLPDDRLSG CQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRVMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPPIGFWQITPSDEFRSGG 240
           AYPEAVLL++PKN  LKGEVDDKYQYSCEN +LKVHGWISTNPP GFWQITPSDEFRSGG
Sbjct: 181 AYPEAVLLTDPKNSALKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           P KQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTS GDDPFW
Sbjct: 241 PLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDFPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEV+SWPYSFPASEDF K DQRG VSGRLLVFDRY+ QD+LP SGAFVGL
Sbjct: 301 LWEDAKIQMMAEVESWPYSFPASEDFSKKDQRGMVSGRLLVFDRYVDQDYLPGSGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDYRCDALINVN 420
           SAPGEVGSWQRECKGYQFWTRADD GYFTINH+ TGDYNLYAWVPGFIGDYRCDAL+NV+
Sbjct: 361 SAPGEVGSWQRECKGYQFWTRADDGGYFTINHVLTGDYNLYAWVPGFIGDYRCDALVNVS 420

Query: 421 QGSCIETGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGSCIE GDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNP++INKLFVNHPDRFRQYGL
Sbjct: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPIYINKLFVNHPDRFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMY 540
           WDRYAELYPDEDLVYTVGTSDY KDWFFA VPRKTNSNSHEGTTWQIKF+LDRVD KSMY
Sbjct: 481 WDRYAELYPDEDLVYTVGTSDYRKDWFFAHVPRKTNSNSHEGTTWQIKFQLDRVDRKSMY 540

Query: 541 TLLVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGSHL 600
            L VAIASATLAELQVRVNDPRS+RPLFTSGLIGRDNSIARHGIHGIYW+YSV +AG HL
Sbjct: 541 MLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYSVSVAGCHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPT 634
           VEG+NTIF TQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 601 VEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 633

BLAST of Spg017051 vs. TAIR 10
Match: AT1G09910.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 1006.1 bits (2600), Expect = 1.2e-293
Identity = 448/633 (70.77%), Postives = 531/633 (83.89%), Query Frame = 0

Query: 1   MSSRGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSS GV L + DR+VV+DNGILQ+TLSKP GIITGI YNG+DN+LEV N E+NRGYWDL 
Sbjct: 44  MSSHGVHLHVHDRYVVMDNGILQVTLSKPGGIITGIEYNGIDNVLEVRNKETNRGYWDLH 103

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WN PG KGIFDVI G +FRVIVE EEQVE+SFLR WDPSLEGK++PLNIDKRFI+LRGSS
Sbjct: 104 WNEPGGKGIFDVISGVTFRVIVETEEQVEISFLRTWDPSLEGKYIPLNIDKRFIMLRGSS 163

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRRRLMPLPDDRLSGSCQTL 180
           G YSY IYEHLKDWP F++GETRI FKLRKDKF YMAVAD+R+R+MP PDD   G CQTL
Sbjct: 164 GVYSYGIYEHLKDWPGFELGETRIAFKLRKDKFHYMAVADDRKRIMPFPDDLCKGRCQTL 223

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPPIGFWQITPSDEFRSGG 240
            Y EA LL+ P +P+L+GEVDDKYQYSCENKDL+VHGWIS +PP+GFWQITPS+EFRSGG
Sbjct: 224 DYQEASLLTAPCDPRLQGEVDDKYQYSCENKDLRVHGWISFDPPVGFWQITPSNEFRSGG 283

Query: 241 PHKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           P KQ+LTSHVGPTTLA+F S HYAG+ ++P+F  GEPWKKV+GPVF+YLNST+ GDDP  
Sbjct: 284 PLKQNLTSHVGPTTLAVFHSTHYAGKTMMPRFEHGEPWKKVYGPVFIYLNSTANGDDPLC 343

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDFPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LW+DAKI+MMAEV+ WPYSF AS+D+PK ++RGT  GRLL+ DR+++ D + A GA+VGL
Sbjct: 344 LWDDAKIKMMAEVERWPYSFVASDDYPKSEERGTARGRLLIRDRFINNDLISARGAYVGL 403

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDYRCDALINVN 420
           + PG+ GSWQ ECKGYQFW  AD+ GYF+I ++  G+YNLYAWVP FIGDY    ++ V 
Sbjct: 404 APPGDSGSWQIECKGYQFWAIADEAGYFSIGNVRPGEYNLYAWVPSFIGDYHNGTIVRVT 463

Query: 421 QGSCIETGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
            G  IE GD+VYEPPRDGPTLWEIGIPDR ASEF++PDP+P  +N++ V+H DRFRQYGL
Sbjct: 464 SGCMIEMGDIVYEPPRDGPTLWEIGIPDRKASEFFIPDPDPTLVNRVLVHHQDRFRQYGL 523

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMY 540
           W +Y ++YP++DLVYTVG SDY +DWFFA VPRK   + HEGTTWQI F L+ +D K+ Y
Sbjct: 524 WKKYTDMYPNDDLVYTVGVSDYRRDWFFAHVPRK-KGDVHEGTTWQIIFNLENIDQKANY 583

Query: 541 TLLVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGSHL 600
            L VAIASATLAELQ+R+ND  + RPLFT+GLIGRDNSIARHGIHG+Y LY+V I G+ L
Sbjct: 584 KLRVAIASATLAELQIRINDAEAIRPLFTTGLIGRDNSIARHGIHGVYMLYAVNIPGNRL 643

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPT 634
           V+GDNTIF  QPRC  PFQGIMYDYIRLEGPP+
Sbjct: 644 VQGDNTIFLKQPRCNGPFQGIMYDYIRLEGPPS 675

BLAST of Spg017051 vs. TAIR 10
Match: AT1G09890.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 953.7 bits (2464), Expect = 7.2e-278
Identity = 431/617 (69.85%), Postives = 505/617 (81.85%), Query Frame = 0

Query: 17  VDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLVWNSPGSKGIFDVIKGS 76
           +DNGI ++TLSKPDGI+TGI YNG+DNLLEVLN E NRGYWDLVW   G+ G FDVIKGS
Sbjct: 1   MDNGIARVTLSKPDGIVTGIEYNGIDNLLEVLNEEVNRGYWDLVWGGSGTAGGFDVIKGS 60

Query: 77  SFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSSGFYSYAIYEHLKDWPD 136
           +F VIV+NEEQ+ELSF R WDPS EGK VPLNIDKRF++L GSSGFY+YAIYEHLK+WP 
Sbjct: 61  NFEVIVKNEEQIELSFTRKWDPSQEGKAVPLNIDKRFVMLSGSSGFYTYAIYEHLKEWPA 120

Query: 137 FDIGETRITFKLRKDKFQYMAVADNRRRLMPLPDDRLSGSCQTLAYPEAVLLSNPKNPQL 196
           F + ETRI FKLRK+KF YMAV D+R+R MPLPDDRL    Q LAYPEAVLL NP   Q 
Sbjct: 121 FSLAETRIAFKLRKEKFHYMAVTDDRQRFMPLPDDRLPDRGQALAYPEAVLLVNPLESQF 180

Query: 197 KGEVDDKYQYSCENKDLKVHGWIST-NPPIGFWQITPSDEFRSGGPHKQSLTSHVGPTTL 256
           KGEVDDKYQYSCENKD+ VHGWI T  P +GFW ITPS E+R+GGP KQ+LTSHVGPT L
Sbjct: 181 KGEVDDKYQYSCENKDITVHGWICTEQPSVGFWLITPSHEYRTGGPQKQNLTSHVGPTAL 240

Query: 257 AIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGD-DPFWLWEDAKIQMMAEVQ 316
           A+F+SAHY G+DLVPKF  GE WKKVFGPVF+YLNS++  D DP WLW+DAK QM  E +
Sbjct: 241 AVFISAHYTGEDLVPKFSEGEAWKKVFGPVFVYLNSSTDDDNDPLWLWQDAKSQMNVEAE 300

Query: 317 SWPYSFPASEDFPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGLSAPGEVGSWQRECK 376
           SWPYSFPAS+D+ K +QRG V GRLLV DRY+ +D + A+  +VGL+ PG  GSWQRECK
Sbjct: 301 SWPYSFPASDDYVKTEQRGNVVGRLLVQDRYVDKDFIAANRGYVGLAVPGAAGSWQRECK 360

Query: 377 GYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDYRCDALINVNQGSCIETGDLVYEP 436
            YQFWTR D+ G+F I+ I  G YNLYAW+PGFIGDY+ D +I +  G  I   DLVY+P
Sbjct: 361 EYQFWTRTDEEGFFYISGIRPGQYNLYAWIPGFIGDYKYDDVITITSGCYIYVEDLVYQP 420

Query: 437 PRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGLWDRYAELYPDEDLV 496
           PR+G TLWEIG PDRSA+EFYVPDPNP +IN L+ NHPDRFRQYGLW+RYAELYPD+DLV
Sbjct: 421 PRNGATLWEIGFPDRSAAEFYVPDPNPKYINNLYQNHPDRFRQYGLWERYAELYPDKDLV 480

Query: 497 YTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMYTLLVAIASATLAEL 556
           Y VG+SDY KDWF+AQV RK ++ +++GTTWQIKF+L  +D    YTL VAIASAT +EL
Sbjct: 481 YVVGSSDYRKDWFYAQVTRKKDNKTYQGTTWQIKFELKNIDKNHSYTLRVAIASATFSEL 540

Query: 557 QVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGSHLVEGDNTIFFTQPRC 616
           Q+RVN+  +  P+FTSGLIGRDNSIARHGIHG+YWL++V +AGS L+EG+NT+F TQPR 
Sbjct: 541 QIRVNNANA-SPMFTSGLIGRDNSIARHGIHGLYWLFNVEVAGSKLLEGENTLFLTQPRS 600

Query: 617 TSPFQGIMYDYIRLEGP 632
           TSPFQGIMYDYIR E P
Sbjct: 601 TSPFQGIMYDYIRFEAP 616

BLAST of Spg017051 vs. TAIR 10
Match: AT4G24430.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 880.2 bits (2273), Expect = 1.0e-255
Identity = 403/638 (63.17%), Postives = 500/638 (78.37%), Query Frame = 0

Query: 6   VRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLVWN--- 65
           V+L +++ HVV+ NG +++T+SKPDG +TGI Y GVDNLLE  N + NRGYWDLVW+   
Sbjct: 7   VQLDVQESHVVMGNGKVKVTISKPDGFVTGISYQGVDNLLETHNEDFNRGYWDLVWSDEG 66

Query: 66  SPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSSGF 125
           +PG+ G  + IKG+SF V+VENEE VE+SF R WD SL+    P+N+DKRFI+ +  +GF
Sbjct: 67  TPGTTGKSERIKGTSFEVVVENEELVEISFSRKWDSSLQDSIAPINVDKRFIMRKDVTGF 126

Query: 126 YSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRRRLMPLPDDRLSGSCQTLAY 185
           YSYAI+EHL +WP F++ +TRI +KLRKDKF+YMA+ADNR+R MPLP+DRL    + LAY
Sbjct: 127 YSYAIFEHLAEWPAFNLPQTRIVYKLRKDKFKYMAIADNRQRKMPLPEDRLGKRGRPLAY 186

Query: 186 PEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPPIGFWQITPSDEFRSGGPH 245
           PEAVLL +P   + KGEVDDKY+YS ENKDLKVHGWIS N  +G WQI PS+EFRSGG  
Sbjct: 187 PEAVLLVHPVEDEFKGEVDDKYEYSSENKDLKVHGWISHNLDLGCWQIIPSNEFRSGGLS 246

Query: 246 KQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNS-TSAGDDPFWL 305
           KQ+LTSHVGP +LA+FLSAHYAG+D+V K +AG+ WKKVFGPVF YLN       DP  L
Sbjct: 247 KQNLTSHVGPISLAMFLSAHYAGEDMVMKVKAGDSWKKVFGPVFTYLNCLPDKTSDPLSL 306

Query: 306 WEDAKIQMMAEVQSWPYSFPASEDFPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGLS 365
           W+DAK QM+ EVQSWPY FPASEDFP  D+RG +SGRLLV D+++S D LPA+GAFVGL+
Sbjct: 307 WQDAKNQMLTEVQSWPYDFPASEDFPVSDKRGCISGRLLVCDKFLSDDFLPANGAFVGLA 366

Query: 366 APGEVGSWQRECKGYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDYRCDALINVNQ 425
            PGEVGSWQ E KGYQFWT AD  GYF IN I  G+YNL  +V G+IGDY+ + LIN+  
Sbjct: 367 PPGEVGSWQLESKGYQFWTEADSDGYFAINDIREGEYNLNGYVTGWIGDYQYEQLINITA 426

Query: 426 GSCIETGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGLW 485
           G  I+ G++VYEPPRDGPT+WEIGIPDRSA+EF+VPDPNP +INKL++ HPDRFRQYGLW
Sbjct: 427 GCDIDVGNIVYEPPRDGPTVWEIGIPDRSAAEFFVPDPNPKYINKLYIGHPDRFRQYGLW 486

Query: 486 DRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMYT 545
           +RY ELYP EDLV+T+G SDY KDWFFA V RK   ++++ TTWQIKFKL+ V     Y 
Sbjct: 487 ERYTELYPKEDLVFTIGVSDYKKDWFFAHVTRKMGDDTYQKTTWQIKFKLENVQKSCTYK 546

Query: 546 LLVAIASATLAELQVRVNDPRSRR--PLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGSH 605
           + +A+A+A +AELQVR+ND  + +  P+FT+G+IG DN+IARHGIHGIY LY+V +    
Sbjct: 547 IRIALATANVAELQVRMNDDDTEKTTPIFTTGVIGHDNAIARHGIHGIYRLYNVDVPSEK 606

Query: 606 LVEGDNTIFFTQPRCTS-PFQGIMYDYIRLEGPPTNLY 637
           LVEGDNT+F TQ   T+  F G+MYDYIRLEGPP + Y
Sbjct: 607 LVEGDNTLFLTQTMTTTGAFNGLMYDYIRLEGPPLDSY 644

BLAST of Spg017051 vs. TAIR 10
Match: AT1G09880.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 826.2 bits (2133), Expect = 1.7e-239
Identity = 375/628 (59.71%), Postives = 478/628 (76.11%), Query Frame = 0

Query: 17  VDNGILQLTLSKPDGIITGIRYNGVDNLLE-VLNPESNRGYWDLVWNSPG-----SKGIF 76
           ++N  LQLTLS P+G +TGI+YNG+DN+L    N E +RGYWD+VWN PG     +KG  
Sbjct: 1   MENRFLQLTLSNPEGFVTGIQYNGIDNVLAYYTNKEYDRGYWDVVWNFPGKKAKKTKGTL 60

Query: 77  DVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSSGFYSYAIYEH 136
           D I+ +   VI +N+E++ELSF R W+ S     VP+NIDKRF++L+ SSGFYSYAI+E 
Sbjct: 61  DRIEATKMEVITQNDEKIELSFTRTWNTS-STTAVPVNIDKRFVMLQNSSGFYSYAIFER 120

Query: 137 LKDWPDFDIGETRITFKLRKDKFQYMAVADNRRRLMPLPDDRLSGSCQTLAYPEAVLLSN 196
           L+ WP  ++   R+ FKL K KF YMA++D+R+R MP+PDDR+    Q LAYPEAV L +
Sbjct: 121 LQGWPAVELDNMRLVFKLNKKKFHYMAISDDRQRYMPVPDDRVPPRGQPLAYPEAVQLLD 180

Query: 197 PKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPPIGFWQITPSDEFRSGGPHKQSLTSHV 256
           P  P+ KGEVDDKY+YS E+KD+KVHGWISTN  +GFWQITPS+EFRS GP KQ L SHV
Sbjct: 181 PIEPEFKGEVDDKYEYSMESKDIKVHGWISTNDSVGFWQITPSNEFRSAGPLKQFLGSHV 240

Query: 257 GPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFWLWEDAKIQMM 316
           GPT LA+F S HY G DL+  F+ GE WKKVFGPVF+YLNS   G DP  LW +AK Q  
Sbjct: 241 GPTNLAVFHSTHYVGADLIMSFKNGEAWKKVFGPVFIYLNSFPKGVDPLLLWHEAKNQTK 300

Query: 317 AEVQSWPYSFPASEDFPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGLSAPGEVGSWQ 376
            E + WPY+F AS+DFP  DQRG+VSGRLLV DR++S + +PA+G++VGL+APG+VGSWQ
Sbjct: 301 IEEEKWPYNFTASDDFPASDQRGSVSGRLLVRDRFISSEDIPANGSYVGLAAPGDVGSWQ 360

Query: 377 RECKGYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDYRCDALINVNQGSCIETGDL 436
           RECKGYQFW++AD+ G F+IN++ +G YNLYA+ PGFIGDY  D + +++ GS I  GDL
Sbjct: 361 RECKGYQFWSKADENGSFSINNVRSGRYNLYAFAPGFIGDYHNDTVFDISPGSKISLGDL 420

Query: 437 VYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGLWDRYAELYPD 496
           VYEPPRDG TLWEIG+PDRSA+EFY+PDPNP  +NKL++NH D++RQYGLW+RY+ELYPD
Sbjct: 421 VYEPPRDGSTLWEIGVPDRSAAEFYIPDPNPSFVNKLYLNHSDKYRQYGLWERYSELYPD 480

Query: 497 EDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLD--RVDHKSMYTLLVAIAS 556
           ED+VY V   DYSK+WFF QV RK  +  ++GTTWQI+F+ D    +    + L +A+A+
Sbjct: 481 EDMVYNVDIDDYSKNWFFMQVTRKQANGGYKGTTWQIRFQFDDKMKNVTGNFKLRIALAT 540

Query: 557 ATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGSHLVEGDNTIF 616
           + +AELQVRVND  +  PLF +  IGRDN+IARHGIHG+YWLYSV +  + L  G+NTI+
Sbjct: 541 SNVAELQVRVNDLSADPPLFRTEQIGRDNTIARHGIHGLYWLYSVNVPAASLHVGNNTIY 600

Query: 617 FTQPRCTSPFQGIMYDYIRLEGPPTNLY 637
            TQ   TSPFQG+MYDYIRLE P +  Y
Sbjct: 601 LTQALATSPFQGLMYDYIRLEYPDSINY 627

BLAST of Spg017051 vs. TAIR 10
Match: AT2G22620.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 784.6 bits (2025), Expect = 5.8e-227
Identity = 362/618 (58.58%), Postives = 453/618 (73.30%), Query Frame = 0

Query: 15  VVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLVWNSPGSKGIFDVIK 74
           VVVDNGI+Q+T S P+G+ITGI+Y+G+DN+L+  +   +RGYWD+VW  P  K   D ++
Sbjct: 62  VVVDNGIVQVTFSNPEGLITGIKYHGIDNVLD--DKIDDRGYWDVVWYEPEKKQKTDKLE 121

Query: 75  GSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSSGFYSYAIYEHLKDW 134
           G+ F +I +NEEQ+E+SF R W  S  G  VPLN+DKR+I+  G SG Y Y I E L+ W
Sbjct: 122 GTKFEIITQNEEQIEISFTRTWTISRRGSLVPLNVDKRYIIRSGVSGLYMYGILERLEGW 181

Query: 135 PDFDIGETRITFKLRKDKFQYMAVADNRRRLMPLPDDRLSGSCQTLAYPEAVLLSNPKNP 194
           PD D+ + RI FKL   KF +MA++D+R+R MP   DR   + ++LAY EAVLL+NP NP
Sbjct: 182 PDVDMDQIRIVFKLNPKKFDFMAISDDRQRSMPSMADR--ENSKSLAYKEAVLLTNPSNP 241

Query: 195 QLKGEVDDKYQYSCENKDLKVHGWISTNPPIGFWQITPSDEFRSGGPHKQSLTSHVGPTT 254
             KGEVDDKY YS E+KD  VHGWIS++PP+GFW ITPSDEFR GGP KQ LTSH GP T
Sbjct: 242 MFKGEVDDKYMYSMEDKDNNVHGWISSDPPVGFWMITPSDEFRLGGPIKQDLTSHAGPIT 301

Query: 255 LAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFWLWEDAKIQMMAEVQ 314
           L++F S HYAG+++   +R GEPWKKVFGPV  YLNS S  D    LW DAK QM AEV+
Sbjct: 302 LSMFTSTHYAGKEMRMDYRNGEPWKKVFGPVLAYLNSVSPKDSTLRLWRDAKRQMAAEVK 361

Query: 315 SWPYSFPASEDFPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGLSAPGEVGSWQRECK 374
           SWPY F  SED+P   QRGT+ G+ L+ D Y+S+  +    AFVGL+  GE GSWQ E K
Sbjct: 362 SWPYDFITSEDYPLRHQRGTLEGQFLIKDSYVSRLKIYGKFAFVGLAPIGEAGSWQTESK 421

Query: 375 GYQFWTRADDRGYFTINHIHTGDYNLYAWVPGFIGDYRCDALINVNQGSCIETGDLVYEP 434
           GYQFWT+AD RG F I ++  G+Y+LYAW  GFIGDY+ +  I +  GS +  G LVYEP
Sbjct: 422 GYQFWTKADRRGRFIIENVRAGNYSLYAWGSGFIGDYKYEQNITITPGSEMNVGPLVYEP 481

Query: 435 PRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNH-PDRFRQYGLWDRYAELYPDEDL 494
           PR+GPTLWEIG+PDR+A EFY+PDP P  +NKL+VN   DRFRQYGLWDRYA+LYP  DL
Sbjct: 482 PRNGPTLWEIGVPDRTAGEFYIPDPYPTLMNKLYVNPLQDRFRQYGLWDRYADLYPQNDL 541

Query: 495 VYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMYTLLVAIASATLAE 554
           VYT+G SDY  DWFFA V R   +++++ TTWQI F L  V+    YTL +A+ASA  +E
Sbjct: 542 VYTIGVSDYRSDWFFAHVARNVGNDTYQPTTWQIIFNLKNVNRIGRYTLRIALASAADSE 601

Query: 555 LQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGSHLVEGDNTIFFTQPR 614
           LQ+R+NDP+S   +FT+G IG+DN+IARHGIHG+Y LYS+ +AG+ L  GDNTIF TQ R
Sbjct: 602 LQIRINDPKS-DAIFTTGFIGKDNAIARHGIHGLYRLYSIDVAGNLLSVGDNTIFLTQTR 661

Query: 615 CTSPFQGIMYDYIRLEGP 632
             +PFQGIMYDYIRLE P
Sbjct: 662 SRTPFQGIMYDYIRLESP 674

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038897922.10.0e+0093.53probable rhamnogalacturonate lyase B [Benincasa hispida][more]
XP_004136250.10.0e+0092.89probable rhamnogalacturonate lyase B isoform X1 [Cucumis sativus] >KGN60276.1 hy... [more]
XP_022980958.10.0e+0092.11probable rhamnogalacturonate lyase B [Cucurbita maxima][more]
XP_022940913.10.0e+0091.96probable rhamnogalacturonate lyase B isoform X1 [Cucurbita moschata][more]
XP_008466137.10.0e+0092.58PREDICTED: probable rhamnogalacturonate lyase B isoform X1 [Cucumis melo] >KAA00... [more]
Match NameE-valueIdentityDescription
Q5AZ851.4e-2022.02Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 ... [more]
A5ABH42.3e-1826.33Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / F... [more]
Match NameE-valueIdentityDescription
A0A0A0LJQ90.0e+0092.89Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_3G892770 PE=3 SV=... [more]
A0A6J1J0S60.0e+0092.11Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111480252 PE=3 SV... [more]
A0A6J1FQM10.0e+0091.96Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111446358 PE=3 ... [more]
A0A5D3E6J10.0e+0092.58Rhamnogalacturonan endolyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3CQJ40.0e+0092.58Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103503644 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G09910.11.2e-29370.77Rhamnogalacturonate lyase family protein [more]
AT1G09890.17.2e-27869.85Rhamnogalacturonate lyase family protein [more]
AT4G24430.11.0e-25563.17Rhamnogalacturonate lyase family protein [more]
AT1G09880.11.7e-23959.71Rhamnogalacturonate lyase family protein [more]
AT2G22620.15.8e-22758.58Rhamnogalacturonate lyase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.60.120.260coord: 438..629
e-value: 1.6E-20
score: 75.3
NoneNo IPR availableGENE3D2.60.40.1120coord: 329..423
e-value: 5.7E-6
score: 28.5
NoneNo IPR availablePANTHERPTHR32018RHAMNOGALACTURONATE LYASE FAMILY PROTEINcoord: 1..633
NoneNo IPR availablePANTHERPTHR32018:SF8RHAMNOGALACTURONATE LYASE FAMILY PROTEINcoord: 1..633
NoneNo IPR availableCDDcd10320RGL4_Ncoord: 6..297
e-value: 1.22969E-80
score: 253.85
NoneNo IPR availableCDDcd10317RGL4_Ccoord: 442..629
e-value: 2.64695E-55
score: 183.246
IPR029411Rhamnogalacturonan lyase, domain IIIPFAMPF14683CBM-likecoord: 440..628
e-value: 4.1E-56
score: 189.5
IPR029413Rhamnogalacturonan lyase, domain IIPFAMPF14686fn3_3coord: 354..425
e-value: 1.4E-23
score: 82.5
IPR029413Rhamnogalacturonan lyase, domain IICDDcd10316RGL4_Mcoord: 331..430
e-value: 3.5124E-33
score: 120.431
IPR014718Glycoside hydrolase-type carbohydrate-bindingGENE3D2.70.98.10coord: 7..305
e-value: 1.5E-12
score: 49.4
IPR010325Rhamnogalacturonate lyasePFAMPF06045Rhamnogal_lyasecoord: 10..200
e-value: 4.1E-90
score: 300.9
IPR013784Carbohydrate-binding-like foldSUPERFAMILY49452Starch-binding domain-likecoord: 329..421
IPR011013Galactose mutarotase-like domain superfamilySUPERFAMILY74650Galactose mutarotase-likecoord: 12..296
IPR008979Galactose-binding-like domain superfamilySUPERFAMILY49785Galactose-binding domain-likecoord: 437..629

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg017051.1Spg017051.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0102210 rhamnogalacturonan endolyase activity
molecular_function GO:0003824 catalytic activity