Spg016728 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg016728
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein KAKU4
Locationscaffold9: 42963011 .. 42970263 (-)
RNA-Seq ExpressionSpg016728
SyntenySpg016728
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AACCGAAGACCACCACTACAGACACTGAGAAACAACATCAAAATGGATCACGCACGATTGAGCGTTTGCAGAAATGATCAAAGCCAAGAATGTAATTTACTACACAGCCCCTTCCCATGCAGTACAAAACCAAAATTGCACAAAGGGTAGAATTGTCTTTTCGGAATTACAAAGCGTCAATTTTCAATTGCTTTCCCGGAGTGGGTTACTTCTCTTCTCCCTTCCATTACTGGAAAGACCCCTTCTCGCCTGAGCGACCAAGTTCTTCACTGTGAACTTCCATGGCGTCTCTTCCTGCATATCGCGAGGCCGCCGGGTCAAGATCCGGCGGGAAAATTGTTCGAGCTAGGCGAGTTCGAAGTAGAAACCATCCTTACGAGCGTCCTGGACTTTCGAAGTTGGGCCCTGGAGGAAACCCTAGTTGGCTCTCCAGGTTCATTTTCTCGCCTACTCTTACCATTGCTACCGGCGCCGGGAAGCTGCTCTCCTCTGTCTTCGTTTCCGACTCATCGTCCTCCTCTTCCGACAGCGATTCAGGTTGGCTTAACTTAACTGAATTTTTGTGTATGTTTTTTCAGAATTATGCGGATGGAGTAGGTTTTTTTAAAAAAAAAAAACTAAGAGTTGGGAGTGGTTAATGCGTCTGTGGTTGTTTCTTTGATGTGCTGCGGAATGTAAGCTTGTTTTGAGGATATGAGAAGTAGTAGGAAGAGTACTGAAGTTAGGCACTAGCTCAAGAAGGGTGAAATGTAGTTCGAGCTTTGAAGGGGGATGGGAAATGGAGGAAGTGCTTGAAAATAAGTAAGCAGCACTTCGTGAACTTAATGGTGTATGCTGTTAAGTATACTTAGTAAGCTGGGTAAGAATTCTGGAATTGATGAACTTAGGTGGTATGATGTGCTTGGGTTTCATAAAATTTTTGTTTTCAGTTGTATGATATGTTTTCCTAGTGGAACAATTTGATTCGTATTTAGGATGCTTAATTAGCCGATTTCTTTCATTTACCTACCTAGTTTAGATTTGTGGTTGGGCTTGCAATATTTTCAATTAGCATTTTATAAGTAACTTAAAAGTGTAATTCTACGTTTCCAGTCTTATCGCTTCTATTTCAAGGTCATGTCATGATGTTCACTGAAGTTTGATCTATTTCAGACCAAGAACAGCATTTTCATTGTTTTAGTGAAAAGTTACAATCAAGAATCTATACTGTATGGGAGCTTATAAGAATTCACCCCAATTGGAATCAATTACGTTAAGACAGAAGTATAATTATAAAATTCCTTAGGGAACCTTGTTAAGAACAACGAAACTTTCTAGCACCCTGTAAATTTAGAGTTGGTGAGAAAATGAACTTGGAGATTCAATTAGGGTTTCCTTCAGGGCCCAAATTTGAAGATCTGAGACGATCTTAAGGAGTCTTTAACTCCTAGCTTGCTTGGCTCGAATCATGTTTCCATTGGATCTTGGCCCATGGCATTGAGGTATGTAGGTCCAAACACCATTGAAGCCGCATACTCAAGAAGCTTAGACATGACGTTTCCAAATTGGTTTTTAGCCCTGAGACCATCTCAAATAACTTTAATACATTAAATATATTTTGAATATATATATATATATATATATTTTAATAGAAAAACCAGCTTTCATTGAGAAAAAGATGAAAGAATACAAGGGCATAAAAAAAAAAAAAACCCCACAAAAGGGAGTCCCCTTAAAGGAAGGTGCTCCAATCCAATAAGATAAGACCTAAAGGATAATTACACAATAAACTCGTAACTGAGGCCCAAAGGGAAGCATGGAATCTCGATAAAGACCATACATCACCTGGGCGTCTAACTATCCCCCTAAAAACCCTGTTATTTCTCTCTCCCCATAAGTTCCATATGAGTGCACACATGCCAGCTTGCCATAAAAATCTTCCTTTGCCATGGTGGGTCGGATTGAGGAGAAACTCTTCGATCAACACTCTCAAATCCGGTTTGGTCAGTATCCTAGAAGATAATTGTATCATCTTGACTTGAAAGTGAATTGCGTGAACCCTCCTCTTACCAACTCAAAAACCTTCCAAGAATCCCTTTTGCACTTTCTGACTGATCATGCTACCAAGAACGCCTCTTACAATGACAAAGAGAAAGGAAGAAAGTTGGTCCCCTTATCTTAGACTCTTTGATGCCACTACTATTTCTCTTGGTTTCCATTGACAAATGTTGAAAATTTAGTATCTGAAATACATCCCTATTCCATGCTAGCCATTCACGTCCAGACTTATTTCTTCAATGATATCATTTATCATTGACATATTTCTGCTTAGTGTGTGCAGAAAACATGTGATTTCATTTGCAATTGAAAGTTGATTTAAAACTTAATTCTTGCATGTTACAATAGGAAAGCAACAATATGTCAATTCATATAATATACGTACAGGCTTATGTAAGATTAATGGACTAAATTGATGACTAATCATACGCCTTTTATCTTGGATGTTTCATATGGTTTCTTCAAGTTCCTTGCACATTTTTCATTTTCATTTCACATTTATTTATGACAAAATTCAATGCATAGGAATTGTATAGTTTTATTATTCATTTAATTGACAAGTTCTCTGCTTTCGTTTTGCTCATATGGCTCTTTTAAAGATGTATTGTATCTTATTTTAACAGAGGATGGTGTTGAAGATGATGTTCATGATGAAAATCGTGTCTTCCAGGGAACTGAAGGAGTGAAGAAGGTAAACAATCTAGATGTTAAATCATGTACTTGTTTAGGTGTTTGTCTCATAAATATTGAAATTAGGTTGTTTGTCTTACTATTGGACAACATAAGAAATGTCTCGATATTTTTCTAGCAAACCTGCTAGTTTGATATGAGATTGAGTGTGGCAATATTATGTTTTAATAACATTATCCTTATTTTAATTCTTTTTATGTTGAAAGAAAAAGTTTTAATGGTTAAGTGACGCAGCATTGTATTAGCTTATGGTTTGAGGGTCAGCTAGAGCTATGTGGGTATACAGATTAATAAAATATATGAAGGGAAGATCTATTGTGGAGTCAACATGATAGTTATATATGCATCAAATATCTGACAAAATCAAAGTGTAAAACAATGGATTATTTGGTGTTTGTTTAATAGACTACAACATCATATCTGATTGAGATTTTTATGTTGGAAGAATGGGAAATCGGAGATGGTCAATTTATTTAGGAAGGACTTTCCACCAGAGAAGAAAGATAGCAAGCATCTAATTGAACAGCTACTGATGCAGGAGACCTTCTCTAGGTACGACATGATTAAGTCATTGTGTTGGTGCGACATGTAAATTGGTCTAGTGATTTCTTATGTTATTCTCTATCAATTAACCTGAGAAAAGAATTTTCGTTTTTGTTTCCTGTGAACTGTGATTAACAATGCTCATCTTCGTTTTACTAGCTTGCTTGTACTTTTATCTTGGTTTCAGTTTATCTAGACTTCCTGCACATGCTGAAATAAGTTATTTTTCCTGTTCAAACTGAAGTTCCTATATGTTCTCATGGTTAAATAGTTTAGTTCATGAGTGACAAATGACAGTACATTGAACTAGGTTTTCTCAACTACCACTTAGATGGTGGCCTTCAATTATTTGTTACTAGCGGCCTCTGGTTCATTCTCACTTTTATTCAAGCAGGGCAGAGCGTGACAAGTTATTTCAGATAATTGAATCAAGGGTTGTAGAATGTCAAACCATTGAAGGTGATGCTGCAGGGAGGCTGACTGAGATAACAAGCAGAGAAGTTGATGATGGTACTGACCTTCCAATTTCCTGTAAACCACAATTTTAATTTTATGCAAATAATGTGACTCTTGTTAATATTTAGGGAAATTTTTTTTTAAGGAGCGTATTTTTTTTATAAGAAATTTAAACCTTATTACCAGATTAAGATGTTGTACAAATTGATACAGATGATGACAGTCCTGCAGTTTGCAGCACAGCAATTCTCGAGGCAAAGAAATGGTTGAATGAAAAACGATTGGGATTGGGATCTAGTTCAACGTTGGAACTGGATCATGGGACATGCACCTTGAATTCCACGATGTTGCCAGTGGTTGGTGATTCTTTCTTTTCTTGTTCTTGAGTCTTGAGTGGCATATGAATAATCACGCAGTGGATGCAACTTTCACTTACATTAAAGTTCAATATTCCTCTCAGTTCACTTGTTATGGACTCCTTTTCTGAGCAAATGATTTATTTGTATGCACGACCATATCCTAATTGCCATAAATAAGTTTGATTATTATTTGTTAGTTGTAACTCAATGTCTTTTATGTAGCTATGATTTAGCTTCGATTTGTGTGTTATTTTTATACCCTTCTTAACTAGGGCTGTGATCTTTCCAGCCCCCCCCCTCCCCAAAAAAAAAAAATAGTTTCAATAAGGTTTCATCTCTTACATTCCCTGCTAATATTGAATTATCTACTGCTGCAGGTTAACGATGAAGAAATGGGTTCGCCTGTGGATGTAGCCAAGTCATACATGCGGGCACGTCCTCCATGGGCTTCTCCTTCCACAAATAATTTTGAGTTTAAATCTCCTTCACCATCAGGGTTACAGCTTTTCAAAGAAGAAACATCATATTCAATTAGTGGAAATCCGCTGTCTTCATCTAAGGTACCATTTTATCCATTAAACTACGCTCATGATGCTTCTGAAAAAAATTCCCCTTATATTTCTTCTTCTTCTTCTTTTAATTATGGTTTTTTTACCGTCTTATTTTATTTTATGCTTCTGCTGCTTATTTATAGTGACAGAAGGGTGTTTTTGCTCTAAATTAGATGGTTGTCTTTTCAAGTCGTGAGACCATATGAAAGGTCAATTGATAAATATTTTCAAGTTTCTCAAAACGTTGGTAATAAATATTTAGAGGAGTGACCAAAATTTAACAGTAGGTAGAATTTTGTGGTTTTTGGTTTTGCCCTTTCACCTCCCAATCATTCATGTATGCTTGTAAGAACAAGAGCATGTATACTTCTACAACTCAACCCGTCATTCTGTTTGTTGACATTGGAGTGCAGATAAAAAGGGAATCTCCTGCCGGTGGATCATGGAACATCCAGGAGGAAATACGACGCGTGCGATCAAGAGCAACCGAGGAAATGCTAAAAACTCCATCTTCCAAACTTGATTGGTCTTCATTTGCGTCAGACTACAGAAGTAATTTGAATTCTACACGTTCTGACTATCTGAAAATTTATAGTGAAGATAAAATGCAACATGCTGTAAAGTCAATAGATAAATCAATGAATTGGTCTGCAGATAATACTGTCACTCACACCTTATCAGGTACGCATTTCTGTGAGTCCAACGATGTACTTACATTTGTAAAAGATAATAAAAGTTTTCTTAATTTTCTATTTGGAATTTGGAATTCCCTAAACATTGTGGACCTCATTTGCGCCCTCACAGAGTCAAAGATCACACAAGATGTGTCGGAAAACGGAGCTTGCCTACTCGATACAATCAGCCCTATTCAGCAACAGGATAAGGTAACAGACTCCCAAAAGGGATTTGTTAGGCCTCCAATTGCGAATGTATATTCTAGGAGGGGCAACTTGCAAAATTGTAGATCTTACCGTTAGTTAAATTTTATTCTTGGTATATATGGGAGGGGATGTGAGCTTGAGATCTAACTTGGACAGTTTCTAGAGTGTAGGGGAGTGACCAGCCCTCTTATATTGGCTGGGTGGCTTGTAGGGTGATCTTCACCCCTTTTTGATATCGCTATACGTAAATAATACTTCATATCTGTCAGGCTTTTGCAAATTACTTATCGTTTGTCGTCTACACGGGTTTGGTTGTCTTGAGAATTATTAACTCAGTGCCTGTTCTATGAAAATTTTGCTAACACTTCCTCTGAGTAGAATGGTATCGATCTTGTTAGATGTTTGATACAGTTCAATTATTCCTTTCCCTTGCAGGATTTGGAAACCAATCCTACCACTGAGAGAAAAGGTACGTAATAGTTTTATCCTATGTTTTAGGCTCCTCGATTGTTGTAGGATTAAGCAGTAGTTCGATTGGATTTATCGGAGAGCAAACATGCAGATCTAGACATTCATGATCATTAAAAAACTATGAGAGCAAAAATTGAAAAACAAAAAAAGGAAAAAACTTTGATCAGCTCCTGTGTAGTATATGCTTCACTAACTAACAATTAAGTAGTAAACAGTATTTGCTTCTCTGTGCAGCATCAAATTCAAGTTTAGATGAGAGAGAATGCTCTACAGCACACGAAGTTACGAGGCTCGCTAATGGTTTTCCTTCAGTACCTAGGTATCTCGATCTTATCTTTCTCGATGTCTGTACCCAAGTGAATAGTGTGTAATAACTGATTTTTTTTTTCTTGCACTGCAGTTCCGGGGAGCTTGCTATAGAACAGAACCATGTCGAGAAGATAGTTGAAGAGAACGATTCATCAGGTCACAACAATGAGGCGAAAGGGCTGGCTTTGGAGGAGCGATGCGAACTATTAAGTGAAGTGTCCATGGAGGTTCCAAATGTGAATGAAAATGATACCGATAAAGTTCTATCAGATGGAAACGATGTCGGTAAAGTTGCATCGGAAGGAAACGATAATGGTGCTAGCAAGGAAAAAGATGGTGCTGGTAATGTCAATAAAGTTGAGAAGCCAAGTTCACGAAGCAACGTGGCAGCAGGGAAAACTAGCGGAACGGCATATCTGCGGCGAGGAAGACGAAGAAACTGAGGGGGGGGGGGGGGGGGGAACAAGAATGTAATTTGTGTTGAATTTTTGTTTGGAATCAAATATTAAGGATAAAACTTTTGACCATAGCTAAGGCAAGGCTGTTGTATCATCAGTAGTTTTGATTAGTTGTACGGATTTGTATAGAGCTAGATCATAGAATCAGCTTGAATAAGCTACACCTAAGAAGCTTTATGTATTTAAGTTGTGGAAAAATTCTGTGTTAGAAATGGCTAAATCTAGCTTCTTTATCCATGTTTTTTCTCTCCAAGAACTGTTTTTCTTTGTTCAAATATCATTTTGGTCTTGGAACTTTAGGGGTTGTTCTCCTTTATTTCTTGTATTTTATGGATTGCTTTCTTACTTTCAAAGATCTTAGATTTAGTATATAACTGTAGGTGGTTTTGGCATGGCGTTTTGTCATTGTAGGCGGTTTTTGAACACGCGCACTGTGTTGCGAGGAATGCTTTGGATTTCTCTTCGTCTTCAGGCGATTTCCATGGTAGCTGCTCCTAG

mRNA sequence

ATGGCGTCTCTTCCTGCATATCGCGAGGCCGCCGGGTCAAGATCCGGCGGGAAAATTGTTCGAGCTAGGCGAGTTCGAAGTAGAAACCATCCTTACGAGCGTCCTGGACTTTCGAAGTTGGGCCCTGGAGGAAACCCTAGTTGGCTCTCCAGGTTCATTTTCTCGCCTACTCTTACCATTGCTACCGGCGCCGGGAAGCTGCTCTCCTCTGTCTTCGTTTCCGACTCATCGTCCTCCTCTTCCGACAGCGATTCAGAGGATGGTGTTGAAGATGATGTTCATGATGAAAATCGTGTCTTCCAGGGAACTGAAGGAGTGAAGAAGAATGGGAAATCGGAGATGGTCAATTTATTTAGGAAGGACTTTCCACCAGAGAAGAAAGATAGCAAGCATCTAATTGAACAGCTACTGATGCAGGAGACCTTCTCTAGGTACGACATGATTAAGGCAGAGCGTGACAAGTTATTTCAGATAATTGAATCAAGGGTTGTAGAATGTCAAACCATTGAAGGTGATGCTGCAGGGAGGCTGACTGAGATAACAAGCAGAGAAGTTGATGATGATGATGACAGTCCTGCAGTTTGCAGCACAGCAATTCTCGAGGCAAAGAAATGGTTGAATGAAAAACGATTGGGATTGGGATCTAGTTCAACGTTGGAACTGGATCATGGGACATGCACCTTGAATTCCACGATGTTGCCAGTGGTTAACGATGAAGAAATGGGTTCGCCTGTGGATGTAGCCAAGTCATACATGCGGGCACGTCCTCCATGGGCTTCTCCTTCCACAAATAATTTTGAGTTTAAATCTCCTTCACCATCAGGGTTACAGCTTTTCAAAGAAGAAACATCATATTCAATTAGTGGAAATCCGCTGTCTTCATCTAAGATAAAAAGGGAATCTCCTGCCGGTGGATCATGGAACATCCAGGAGGAAATACGACGCGTGCGATCAAGAGCAACCGAGGAAATGCTAAAAACTCCATCTTCCAAACTTGATTGGTCTTCATTTGCGTCAGACTACAGAAGTAATTTGAATTCTACACGTTCTGACTATCTGAAAATTTATAGTGAAGATAAAATGCAACATGCTGTAAAGTCAATAGATAAATCAATGAATTGGTCTGCAGATAATACTGTCACTCACACCTTATCAGAGTCAAAGATCACACAAGATGTGTCGGAAAACGGAGCTTGCCTACTCGATACAATCAGCCCTATTCAGCAACAGGATAAGGATTTGGAAACCAATCCTACCACTGAGAGAAAAGCATCAAATTCAAGTTTAGATGAGAGAGAATGCTCTACAGCACACGAAGTTACGAGGCTCGCTAATGGTTTTCCTTCAGTACCTAGTTCCGGGGAGCTTGCTATAGAACAGAACCATGTCGAGAAGATAGTTGAAGAGAACGATTCATCAGGTCACAACAATGAGGCGAAAGGGCTGGCTTTGGAGGAGCGATGCGAACTATTAAGTGAAGTGTCCATGGAGGTTCCAAATGTGAATGAAAATGATACCGATAAAGTTCTATCAGATGGAAACGATGTCGGTAAAGTTGCATCGGAAGGAAACGATAATGGTGCTAGCAAGGAAAAAGATGGTGCTGGTAATGCGGTTTTTGAACACGCGCACTGTGTTGCGAGGAATGCTTTGGATTTCTCTTCGTCTTCAGGCGATTTCCATGGTAGCTGCTCCTAG

Coding sequence (CDS)

ATGGCGTCTCTTCCTGCATATCGCGAGGCCGCCGGGTCAAGATCCGGCGGGAAAATTGTTCGAGCTAGGCGAGTTCGAAGTAGAAACCATCCTTACGAGCGTCCTGGACTTTCGAAGTTGGGCCCTGGAGGAAACCCTAGTTGGCTCTCCAGGTTCATTTTCTCGCCTACTCTTACCATTGCTACCGGCGCCGGGAAGCTGCTCTCCTCTGTCTTCGTTTCCGACTCATCGTCCTCCTCTTCCGACAGCGATTCAGAGGATGGTGTTGAAGATGATGTTCATGATGAAAATCGTGTCTTCCAGGGAACTGAAGGAGTGAAGAAGAATGGGAAATCGGAGATGGTCAATTTATTTAGGAAGGACTTTCCACCAGAGAAGAAAGATAGCAAGCATCTAATTGAACAGCTACTGATGCAGGAGACCTTCTCTAGGTACGACATGATTAAGGCAGAGCGTGACAAGTTATTTCAGATAATTGAATCAAGGGTTGTAGAATGTCAAACCATTGAAGGTGATGCTGCAGGGAGGCTGACTGAGATAACAAGCAGAGAAGTTGATGATGATGATGACAGTCCTGCAGTTTGCAGCACAGCAATTCTCGAGGCAAAGAAATGGTTGAATGAAAAACGATTGGGATTGGGATCTAGTTCAACGTTGGAACTGGATCATGGGACATGCACCTTGAATTCCACGATGTTGCCAGTGGTTAACGATGAAGAAATGGGTTCGCCTGTGGATGTAGCCAAGTCATACATGCGGGCACGTCCTCCATGGGCTTCTCCTTCCACAAATAATTTTGAGTTTAAATCTCCTTCACCATCAGGGTTACAGCTTTTCAAAGAAGAAACATCATATTCAATTAGTGGAAATCCGCTGTCTTCATCTAAGATAAAAAGGGAATCTCCTGCCGGTGGATCATGGAACATCCAGGAGGAAATACGACGCGTGCGATCAAGAGCAACCGAGGAAATGCTAAAAACTCCATCTTCCAAACTTGATTGGTCTTCATTTGCGTCAGACTACAGAAGTAATTTGAATTCTACACGTTCTGACTATCTGAAAATTTATAGTGAAGATAAAATGCAACATGCTGTAAAGTCAATAGATAAATCAATGAATTGGTCTGCAGATAATACTGTCACTCACACCTTATCAGAGTCAAAGATCACACAAGATGTGTCGGAAAACGGAGCTTGCCTACTCGATACAATCAGCCCTATTCAGCAACAGGATAAGGATTTGGAAACCAATCCTACCACTGAGAGAAAAGCATCAAATTCAAGTTTAGATGAGAGAGAATGCTCTACAGCACACGAAGTTACGAGGCTCGCTAATGGTTTTCCTTCAGTACCTAGTTCCGGGGAGCTTGCTATAGAACAGAACCATGTCGAGAAGATAGTTGAAGAGAACGATTCATCAGGTCACAACAATGAGGCGAAAGGGCTGGCTTTGGAGGAGCGATGCGAACTATTAAGTGAAGTGTCCATGGAGGTTCCAAATGTGAATGAAAATGATACCGATAAAGTTCTATCAGATGGAAACGATGTCGGTAAAGTTGCATCGGAAGGAAACGATAATGGTGCTAGCAAGGAAAAAGATGGTGCTGGTAATGCGGTTTTTGAACACGCGCACTGTGTTGCGAGGAATGCTTTGGATTTCTCTTCGTCTTCAGGCGATTTCCATGGTAGCTGCTCCTAG

Protein sequence

MASLPAYREAAGSRSGGKIVRARRVRSRNHPYERPGLSKLGPGGNPSWLSRFIFSPTLTIATGAGKLLSSVFVSDSSSSSSDSDSEDGVEDDVHDENRVFQGTEGVKKNGKSEMVNLFRKDFPPEKKDSKHLIEQLLMQETFSRYDMIKAERDKLFQIIESRVVECQTIEGDAAGRLTEITSREVDDDDDSPAVCSTAILEAKKWLNEKRLGLGSSSTLELDHGTCTLNSTMLPVVNDEEMGSPVDVAKSYMRARPPWASPSTNNFEFKSPSPSGLQLFKEETSYSISGNPLSSSKIKRESPAGGSWNIQEEIRRVRSRATEEMLKTPSSKLDWSSFASDYRSNLNSTRSDYLKIYSEDKMQHAVKSIDKSMNWSADNTVTHTLSESKITQDVSENGACLLDTISPIQQQDKDLETNPTTERKASNSSLDERECSTAHEVTRLANGFPSVPSSGELAIEQNHVEKIVEENDSSGHNNEAKGLALEERCELLSEVSMEVPNVNENDTDKVLSDGNDVGKVASEGNDNGASKEKDGAGNAVFEHAHCVARNALDFSSSSGDFHGSCS
Homology
BLAST of Spg016728 vs. NCBI nr
Match: XP_038898993.1 (protein KAKU4 [Benincasa hispida])

HSP 1 Score: 788.1 bits (2034), Expect = 5.0e-224
Identity = 429/540 (79.44%), Postives = 468/540 (86.67%), Query Frame = 0

Query: 1   MASLPAYREAAGSRSGGKIVRARRVRSRNHPYERPGLSKLGPGGNPSWLSRFIFSPTLTI 60
           MASLPAY EA GSRSGGKIVRARRV+SR  PYERPG S LGPGGNPSW+S+FIFSPT TI
Sbjct: 1   MASLPAYSEAGGSRSGGKIVRARRVQSRKTPYERPGPSNLGPGGNPSWISKFIFSPTRTI 60

Query: 61  ATGAGKLLSSVFVSDSSSSSSDSDSEDGVEDDVHDENRVFQGTEGVKKNGKSEMVNLFRK 120
           ATGAGKLLSSVFVSDSSSSSSDS+SED  EDDVHDEN VFQG EGVKKNG SEMVNLFRK
Sbjct: 61  ATGAGKLLSSVFVSDSSSSSSDSESEDDDEDDVHDENCVFQGAEGVKKNGTSEMVNLFRK 120

Query: 121 DFPPEKKDSKHLIEQLLMQETFSRYDMIKAERDKLFQIIESRVVECQTIEGDAAGRLTEI 180
           DFPP KKDSKHLIEQLLMQETFSR     AERDKLFQIIESRVVECQT EG AAGRLTEI
Sbjct: 121 DFPPVKKDSKHLIEQLLMQETFSR-----AERDKLFQIIESRVVECQTNEGQAAGRLTEI 180

Query: 181 TSREVDDDDDSPAVCSTAILEAKKWLNEKRLGLGSSSTLELDHGTCTLNSTMLPVVNDEE 240
           ++R VD+DD  PAVC TAILEAKKWLNEKRLGLGS+STL+LD G CTLNSTMLPVVN+EE
Sbjct: 181 SNRAVDNDDGRPAVCGTAILEAKKWLNEKRLGLGSTSTLKLDDGPCTLNSTMLPVVNNEE 240

Query: 241 MGSPVDVAKSYMRARPPWASPSTNNFEFKSPSPSGLQLFKEETSYSISGNPLSSSKIKRE 300
           MGSPVDVAKSYM+ARPPWASPSTNNFEFKSPSP GLQLFKEETSYSISGNPLSSS+IKR+
Sbjct: 241 MGSPVDVAKSYMQARPPWASPSTNNFEFKSPSPLGLQLFKEETSYSISGNPLSSSRIKRD 300

Query: 301 SPAGGSWNIQEEIRRVRSRATEEMLKTPSSKLDWSSF--ASDYRSNLNSTRSDYLKIYSE 360
           SP  GSWNIQEE+RRVRS+AT+E+L+TPS+KLDWSSF  ASDY+SNL+S     LKI S 
Sbjct: 301 SPTSGSWNIQEELRRVRSKATKELLRTPSAKLDWSSFASASDYKSNLSS-----LKIPSG 360

Query: 361 DKMQHAVKSIDKSMNWSADNTVTHTLSESKITQDVSENGACLLDTISPIQQQDKDLETNP 420
           DK+QHAVKSIDKSMNWSADNTVTH LSESK  QDVSEN ACLL T S + Q+DKDLETNP
Sbjct: 361 DKIQHAVKSIDKSMNWSADNTVTHNLSESKTMQDVSENEACLLGTTSIVLQRDKDLETNP 420

Query: 421 TTERKASNSSLDERECSTAHEVTRLANGFPSVPSSGELAIEQNHVEKIVEENDSSGHNNE 480
           TTER   NSS D RECST HE   LANGFP +PSS ELA+EQNHV+ IVEEN+SS   +E
Sbjct: 421 TTERDVLNSSFDVRECSTLHEDAGLANGFPPLPSSCELAVEQNHVDHIVEENNSSSRGHE 480

Query: 481 AKGLALEERCELLSEVSMEVPNVNENDTDKVLSDGNDVGKVASEGNDN-GASKEKDGAGN 538
           AK  ++ ERCELLSEVS+EVP++NENDT +V+SDG+D  KV SEGN N   SKE++GAGN
Sbjct: 481 AKEPSVGERCELLSEVSVEVPDMNENDTVRVVSDGSDASKVVSEGNGNYKISKEENGAGN 530

BLAST of Spg016728 vs. NCBI nr
Match: XP_022982016.1 (protein KAKU4-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 782.3 bits (2019), Expect = 2.8e-222
Identity = 430/540 (79.63%), Postives = 472/540 (87.41%), Query Frame = 0

Query: 1   MASLPAYREAAGSRSGGKIVRARRVRSRNHPYERPGLSKLGPGGNPSWLSRFIFSPTLTI 60
           MAS+PAYREA GSR+GGKIVRARR  SR  PYERPGLS LGPGGNPSW+SRFIFSPTLTI
Sbjct: 1   MASVPAYREAGGSRTGGKIVRARRAWSRKTPYERPGLSNLGPGGNPSWISRFIFSPTLTI 60

Query: 61  ATGAGKLLSSVFVSDSSSSSSDSDSEDGVEDDVHDENRVFQGTEGVKKNGKSEMVNLFRK 120
           A+GAGK LSSVF+++SSSSSSDSDSED VEDDVHD+NRVFQG  GVKK+G SEMVN FRK
Sbjct: 61  ASGAGKFLSSVFITESSSSSSDSDSEDDVEDDVHDDNRVFQG-NGVKKSGTSEMVNSFRK 120

Query: 121 DFPPEKKDSKHLIEQLLMQETFSRYDMIKAERDKLFQIIESRVVECQTIEGDAAGRLTEI 180
           DFPPEKKDSKHLIEQLLMQETFSR     AERDKLFQIIESRVVECQ+IEG+AAGRLTE+
Sbjct: 121 DFPPEKKDSKHLIEQLLMQETFSR-----AERDKLFQIIESRVVECQSIEGEAAGRLTEL 180

Query: 181 TSREVDDDDDSPAVCSTAILEAKKWLNEKRLGLGSSSTLELDHGTCTLNSTMLPVVNDEE 240
           +++ VD DD   AVCSTAILEAK WLNEKRLGLGSSSTLELDHG CTLNSTMLP+VNDEE
Sbjct: 181 SNKAVDSDDGGSAVCSTAILEAKTWLNEKRLGLGSSSTLELDHGPCTLNSTMLPLVNDEE 240

Query: 241 MGSPVDVAKSYMRARPPWASPSTNNFEFKSPSPSGLQLFKEETSYSISGNPLSSSKIKRE 300
            GS VDVAKSYMR RP WASPS NNFEFKSPSPS LQLFKEETS+S+SGNPLSSSKIKRE
Sbjct: 241 TGSLVDVAKSYMRERPIWASPSVNNFEFKSPSPSRLQLFKEETSFSVSGNPLSSSKIKRE 300

Query: 301 SPAGGSWNIQEEIRRVRSRATEEMLKTPSS-KLDWSSFASDYRSNLNSTRSDYLKIYSED 360
           SPA GSWNIQEEIRRVRS+ATEEML++ SS KLDWS FASDY+ NL+S RSDY KI+S++
Sbjct: 301 SPASGSWNIQEEIRRVRSKATEEMLRSRSSAKLDWSLFASDYKGNLSSARSDYPKIHSQE 360

Query: 361 KMQHAVKSIDKSMNWSADNTVTHTLSESKITQDVSENGACLLDTISPIQQQDKDLETNPT 420
           KMQHAV+SIDKSMN  AD +VTH LSESKITQDV ENGACLL + S  QQQDKD ETNPT
Sbjct: 361 KMQHAVQSIDKSMNRFADISVTHNLSESKITQDVPENGACLLGSGSIFQQQDKDFETNPT 420

Query: 421 TERKASNSSLDERECSTAHEVTRLANGFPSVPSS-GELAIEQNHVEKIVEENDSSGHNNE 480
            ERKASNSSLDER CST HEV  LANG PS+PSS GELA+E+NHV+KIVEE++SSGH++E
Sbjct: 421 NERKASNSSLDERGCSTVHEVAGLANGLPSLPSSTGELAVEENHVDKIVEESNSSGHDHE 480

Query: 481 AKGLALEERCELLSEVSMEVPNVNEND--TDKVLSDGNDVGKVASEGNDNGASKEKDGAG 537
           AKGL ++ERCE LSEVSMEVPN N+ D   +KV SDGN VGK  SEGN NG SKEK+ AG
Sbjct: 481 AKGLPVKERCE-LSEVSMEVPNTNDADATANKVPSDGNQVGKDVSEGNHNG-SKEKNDAG 532

BLAST of Spg016728 vs. NCBI nr
Match: XP_022139992.1 (protein KAKU4 isoform X2 [Momordica charantia])

HSP 1 Score: 779.2 bits (2011), Expect = 2.3e-221
Identity = 429/560 (76.61%), Postives = 470/560 (83.93%), Query Frame = 0

Query: 1   MASLPAYREAAGSRSGGKIVRARRVRSRNHPYERPGLSKLGPGGNPSWLSRFIFSPTLTI 60
           MA++P+YR+  GSRSGGKIVRARR RSR +PYERPGLS LG GGNPSW+SRFIFSPTLTI
Sbjct: 1   MAAVPSYRDITGSRSGGKIVRARRARSRKNPYERPGLSNLGAGGNPSWISRFIFSPTLTI 60

Query: 61  ATGAGKLLSSVFVSDSSSSSSDSDSEDGVEDDVHDENRVFQGTEGVKKNGKSEMVNLFRK 120
           A+GAGKLLSSVFVSDS SSSSD DSED VE D HDEN  FQG EGVKKNG SE V+LFRK
Sbjct: 61  ASGAGKLLSSVFVSDSDSSSSDGDSEDDVEVDDHDENHFFQGAEGVKKNGTSETVDLFRK 120

Query: 121 DFPPEKKDSKHLIEQLLMQETFSRYDMIKAERDKLFQIIESRVVECQTIEGDAAGRLTEI 180
           DFPP+KKDSKHLIEQLLMQETFSR     AERDKLFQIIESRVVE QTI+ +AAGRLTEI
Sbjct: 121 DFPPQKKDSKHLIEQLLMQETFSR-----AERDKLFQIIESRVVEFQTIDAEAAGRLTEI 180

Query: 181 TSREVDDDDDSPAVCSTAILEAKKWLNEKRLGLGSSSTLELDHGTCTLNSTMLPVVNDEE 240
           ++R VD D+D PAVCSTAILEAKKWLNEKRLGLGSSST ++DHG CTLNSTML V NDEE
Sbjct: 181 SNRAVDSDNDRPAVCSTAILEAKKWLNEKRLGLGSSSTSDMDHGPCTLNSTMLTVANDEE 240

Query: 241 MGSPVDVAKSYMRARPPWASPSTNNFEFKSPSPSGLQLFKEETSYSISGNPLSSSKIKRE 300
           MGSPVDVAKSYMRARPPWASPS+NNFEFKSPSP GLQLFKEETSYSI+GNPLSSSK+KRE
Sbjct: 241 MGSPVDVAKSYMRARPPWASPSSNNFEFKSPSPLGLQLFKEETSYSITGNPLSSSKMKRE 300

Query: 301 SPAGGSWNIQEEIRRVRSRATEEMLKT-PSSKLDWSSFASDYRSNLNSTRSDYLKIYSED 360
           SPA GSWNIQEEIRRVRSRATEEML+T PS+KLDWSS  SDY+SNL+S  SD  KI+SED
Sbjct: 301 SPASGSWNIQEEIRRVRSRATEEMLRTLPSAKLDWSSLTSDYKSNLSSVHSDNPKIHSED 360

Query: 361 KMQHAVKSIDKSMNWSADNTVTHTLSESKITQDVSENGACLLDTISPIQQQDKDLETNPT 420
           KMQHA K IDK + WSADNT TH LSESKITQDVSENGACLLDT + I QQDKDL+TNPT
Sbjct: 361 KMQHAAKLIDKPLYWSADNTATHNLSESKITQDVSENGACLLDTTTIIVQQDKDLQTNPT 420

Query: 421 TERKASNSSLDERECSTAHEVTRLANGFPSVP-SSGELAIEQNHVEKIVEENDSSGHNNE 480
           TERK SNSSLDER+CST HEV  LANGF  VP SSGEL ++     KIVEEN+SSGH++E
Sbjct: 421 TERKVSNSSLDERDCSTVHEVAGLANGFSPVPSSSGELVVD----NKIVEENNSSGHDHE 480

Query: 481 AKGLALEERCELLSEVSMEVPNVNENDTDKVLSDGNDVGKVASEGNDNGASKEKDGAGNA 540
           AKG  +EERCELLSE SMEVPNV E  TDKV S+GND+GK+  E N   ASKEK   G  
Sbjct: 481 AKGPPVEERCELLSEASMEVPNVVEY-TDKVPSEGNDIGKILPEENGATASKEKTDGGK- 540

Query: 541 VFEHAHCVARNALDFSSSSG 559
                + V + +L  S+S G
Sbjct: 541 ----VNNVEKPSLQSSASVG 545

BLAST of Spg016728 vs. NCBI nr
Match: KAG6608200.1 (Protein KAKU4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 778.5 bits (2009), Expect = 4.0e-221
Identity = 432/544 (79.41%), Postives = 472/544 (86.76%), Query Frame = 0

Query: 1   MASLPAYREAAGSRSGGKIVRARRVRSRNHPYERPGLSKLGPGGNPSWLSRFIFSPTLTI 60
           MAS+PAYREA GSR+GGKIVRARR  SR  PYERPGLS LGPGGNPSW+SRFIFSPTLTI
Sbjct: 1   MASVPAYREAGGSRTGGKIVRARRAWSRKTPYERPGLSNLGPGGNPSWISRFIFSPTLTI 60

Query: 61  ATGAGKLLSSVFVSDSSSSSSDSDSEDGVEDDVHDENRVFQGTEGVKKNGKSEMVNLFRK 120
           A+GAGK LSSVF+S+SSSSSSDSDSEDGVEDDVHD+NR FQG  GVKK+G +EMVN FR 
Sbjct: 61  ASGAGKFLSSVFISESSSSSSDSDSEDGVEDDVHDDNRDFQG-NGVKKSGTTEMVNSFR- 120

Query: 121 DFPPEKKDSKHLIEQLLMQETFSRYDMIKAERDKLFQIIESRVVECQTIEGDAAGRLTEI 180
           DFPPEKKD K LIEQLLMQETFSR     AERDKLFQIIESRVVECQ+IEG+AAGRLTE+
Sbjct: 121 DFPPEKKDGKRLIEQLLMQETFSR-----AERDKLFQIIESRVVECQSIEGEAAGRLTEL 180

Query: 181 TSREVDDDDDSPAVCSTAILEAKKWLNEKRLGLGSSSTLELDHGTCTLNSTMLPVVNDEE 240
           +++ VD DD   AVCSTAILEAK WLNEKRLGLGSSS LELDHG CTLNSTMLPVVNDEE
Sbjct: 181 SNKAVDSDDGGSAVCSTAILEAKAWLNEKRLGLGSSSPLELDHGPCTLNSTMLPVVNDEE 240

Query: 241 MGSPVDVAKSYMRARPPWASPSTNNFEFKSPSPSGLQLFKEETSYSISGNPLSSSKIKRE 300
            GS VDVAKSYMR RP WASPS NNFEFKSPSPS LQLFKEETS+S+SGNPLSSSKIKRE
Sbjct: 241 TGSLVDVAKSYMRERPLWASPSANNFEFKSPSPSRLQLFKEETSFSVSGNPLSSSKIKRE 300

Query: 301 SPAGGSWNIQEEIRRVRSRATEEMLKTPSS-KLDWSSFASDYRSNLNSTRSDYLKIYSED 360
           SPA GSWNIQEEIRRVRS+ATEEML++ SS KLDWSSFASDY+ NL+S RSDY KI+SED
Sbjct: 301 SPASGSWNIQEEIRRVRSKATEEMLRSRSSAKLDWSSFASDYKGNLSSARSDYPKIHSED 360

Query: 361 KMQHAVKSIDKSMNWSADNTVTHTLSESKITQDVSENGACLLDTISPIQQQDKDLETNPT 420
           K QHAV+SIDKS+NWSAD +VTH LSESKITQD+ ENGACLL + S IQQQDKD ETNPT
Sbjct: 361 KTQHAVQSIDKSINWSADISVTHDLSESKITQDMPENGACLLGSASIIQQQDKDFETNPT 420

Query: 421 TERKASNSSLDERECSTAHEVTRLANGFPSVPSS-GELAIEQNHVE-KIVEENDSSGHNN 480
            ERKASNSSLDER CST HEV  LANG PS+PSS GELA+E+NHV+ KI EE++SSGH++
Sbjct: 421 NERKASNSSLDERGCSTVHEVAGLANGLPSLPSSTGELAVEENHVDKKIAEESNSSGHDH 480

Query: 481 EAKGLALEERCELLSEVSMEVPNVNEND--TDKVLSDGNDVGKVASEGNDNGASKEKDGA 540
           EAKGL ++ERCE LSEVSMEVPN N+ D   DKV SDGN VGK  SEGN NG SKEK+ A
Sbjct: 481 EAKGLPVKERCE-LSEVSMEVPNANDADATADKVPSDGNHVGKDVSEGNHNG-SKEKNDA 535

BLAST of Spg016728 vs. NCBI nr
Match: XP_022139984.1 (protein KAKU4 isoform X1 [Momordica charantia])

HSP 1 Score: 778.1 bits (2008), Expect = 5.2e-221
Identity = 428/560 (76.43%), Postives = 470/560 (83.93%), Query Frame = 0

Query: 1   MASLPAYREAAGSRSGGKIVRARRVRSRNHPYERPGLSKLGPGGNPSWLSRFIFSPTLTI 60
           MA++P+YR+  GSRSGGKIVRARR RSR +PYERPGLS LG GGNPSW+SRFIFSPTLTI
Sbjct: 1   MAAVPSYRDITGSRSGGKIVRARRARSRKNPYERPGLSNLGAGGNPSWISRFIFSPTLTI 60

Query: 61  ATGAGKLLSSVFVSDSSSSSSDSDSEDGVEDDVHDENRVFQGTEGVKKNGKSEMVNLFRK 120
           A+GAGKLLSSVFVSDS SSSSD DSED VE D HDEN  FQG EGVKKNG SE V+LFRK
Sbjct: 61  ASGAGKLLSSVFVSDSDSSSSDGDSEDDVEVDDHDENHFFQGAEGVKKNGTSETVDLFRK 120

Query: 121 DFPPEKKDSKHLIEQLLMQETFSRYDMIKAERDKLFQIIESRVVECQTIEGDAAGRLTEI 180
           DFPP+KKDSKHLIEQLLMQETFS     +AERDKLFQIIESRVVE QTI+ +AAGRLTEI
Sbjct: 121 DFPPQKKDSKHLIEQLLMQETFSS----RAERDKLFQIIESRVVEFQTIDAEAAGRLTEI 180

Query: 181 TSREVDDDDDSPAVCSTAILEAKKWLNEKRLGLGSSSTLELDHGTCTLNSTMLPVVNDEE 240
           ++R VD D+D PAVCSTAILEAKKWLNEKRLGLGSSST ++DHG CTLNSTML V NDEE
Sbjct: 181 SNRAVDSDNDRPAVCSTAILEAKKWLNEKRLGLGSSSTSDMDHGPCTLNSTMLTVANDEE 240

Query: 241 MGSPVDVAKSYMRARPPWASPSTNNFEFKSPSPSGLQLFKEETSYSISGNPLSSSKIKRE 300
           MGSPVDVAKSYMRARPPWASPS+NNFEFKSPSP GLQLFKEETSYSI+GNPLSSSK+KRE
Sbjct: 241 MGSPVDVAKSYMRARPPWASPSSNNFEFKSPSPLGLQLFKEETSYSITGNPLSSSKMKRE 300

Query: 301 SPAGGSWNIQEEIRRVRSRATEEMLKT-PSSKLDWSSFASDYRSNLNSTRSDYLKIYSED 360
           SPA GSWNIQEEIRRVRSRATEEML+T PS+KLDWSS  SDY+SNL+S  SD  KI+SED
Sbjct: 301 SPASGSWNIQEEIRRVRSRATEEMLRTLPSAKLDWSSLTSDYKSNLSSVHSDNPKIHSED 360

Query: 361 KMQHAVKSIDKSMNWSADNTVTHTLSESKITQDVSENGACLLDTISPIQQQDKDLETNPT 420
           KMQHA K IDK + WSADNT TH LSESKITQDVSENGACLLDT + I QQDKDL+TNPT
Sbjct: 361 KMQHAAKLIDKPLYWSADNTATHNLSESKITQDVSENGACLLDTTTIIVQQDKDLQTNPT 420

Query: 421 TERKASNSSLDERECSTAHEVTRLANGFPSVP-SSGELAIEQNHVEKIVEENDSSGHNNE 480
           TERK SNSSLDER+CST HEV  LANGF  VP SSGEL ++     KIVEEN+SSGH++E
Sbjct: 421 TERKVSNSSLDERDCSTVHEVAGLANGFSPVPSSSGELVVD----NKIVEENNSSGHDHE 480

Query: 481 AKGLALEERCELLSEVSMEVPNVNENDTDKVLSDGNDVGKVASEGNDNGASKEKDGAGNA 540
           AKG  +EERCELLSE SMEVPNV E  TDKV S+GND+GK+  E N   ASKEK   G  
Sbjct: 481 AKGPPVEERCELLSEASMEVPNVVEY-TDKVPSEGNDIGKILPEENGATASKEKTDGGK- 540

Query: 541 VFEHAHCVARNALDFSSSSG 559
                + V + +L  S+S G
Sbjct: 541 ----VNNVEKPSLQSSASVG 546

BLAST of Spg016728 vs. ExPASy Swiss-Prot
Match: Q949W6 (Protein KAKU4 OS=Arabidopsis thaliana OX=3702 GN=KAKU4 PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 7.9e-47
Identity = 140/334 (41.92%), Postives = 188/334 (56.29%), Query Frame = 0

Query: 1   MASLPAYREAAGS-RSGGKIVRARRVRSRNHPYERP-GLSKLGPGGNPSWLSRFIFSPTL 60
           M S+  Y   AG  R GGKIVR RR      P ERP   S+  P  NPSW+SR ++ P  
Sbjct: 1   MDSVSGYAAGAGQPRVGGKIVRPRRTAVVRTPCERPVQRSRDPPQQNPSWISRLVYKPAS 60

Query: 61  TIATGAGKLLSSVFVSDSSSSSSDSDSEDGVEDDVHDENRVFQGTEGVKKNGK--SEMVN 120
            IA+GAGK +SSV  SDSSSSS + +          D +    G E V+KN    +E   
Sbjct: 61  VIASGAGKFISSVVFSDSSSSSEEDE----------DSSSDIDGDEDVEKNNPDFTEEDL 120

Query: 121 LFRKDFPPEKKDSKHLIEQLLMQETFSRYDMIKAERDKLFQIIESRVVECQTIEGDAAGR 180
           L  +    ++  SK +IEQLL+QETF+R      E D+L  II++RVV+  ++       
Sbjct: 121 LSAQQPSIQRLSSKRVIEQLLLQETFAR-----EEGDRLIDIIKARVVDHPSVPSAIE-- 180

Query: 181 LTEITSREVDDDDDSPAVCSTAILEAKKWLNEKRLGLGSSSTLELDHGTCTLNSTMLPVV 240
            T      +  D +   + +TA++EA+KWL EK+   GSSS  +                
Sbjct: 181 -TSHDDYGLTSDVNVGEMSNTAVMEARKWLEEKK--SGSSSKYK---------------A 240

Query: 241 NDEEMGSPVDVAKSYMRARPPWASPSTNNFEFKSPSPSGLQLFKEETSYSISGNPLSSSK 300
            ++  GSPVDVAKSYMRAR PW SP+ NN +F+SPS + +Q     T    S    SSSK
Sbjct: 241 TEDGAGSPVDVAKSYMRARLPWGSPAANNLDFRSPSSARVQ----GTPLPYSAGNFSSSK 295

Query: 301 IKRESPAGGSWNIQEEIRRVRSRATEEMLKTPSS 331
           +KR+S +  SWNIQ+EIR+VR++ATEEMLK+PSS
Sbjct: 301 LKRKSGSNQSWNIQDEIRKVRAKATEEMLKSPSS 295

BLAST of Spg016728 vs. ExPASy TrEMBL
Match: A0A6J1IY54 (protein KAKU4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480997 PE=4 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 1.3e-222
Identity = 430/540 (79.63%), Postives = 472/540 (87.41%), Query Frame = 0

Query: 1   MASLPAYREAAGSRSGGKIVRARRVRSRNHPYERPGLSKLGPGGNPSWLSRFIFSPTLTI 60
           MAS+PAYREA GSR+GGKIVRARR  SR  PYERPGLS LGPGGNPSW+SRFIFSPTLTI
Sbjct: 1   MASVPAYREAGGSRTGGKIVRARRAWSRKTPYERPGLSNLGPGGNPSWISRFIFSPTLTI 60

Query: 61  ATGAGKLLSSVFVSDSSSSSSDSDSEDGVEDDVHDENRVFQGTEGVKKNGKSEMVNLFRK 120
           A+GAGK LSSVF+++SSSSSSDSDSED VEDDVHD+NRVFQG  GVKK+G SEMVN FRK
Sbjct: 61  ASGAGKFLSSVFITESSSSSSDSDSEDDVEDDVHDDNRVFQG-NGVKKSGTSEMVNSFRK 120

Query: 121 DFPPEKKDSKHLIEQLLMQETFSRYDMIKAERDKLFQIIESRVVECQTIEGDAAGRLTEI 180
           DFPPEKKDSKHLIEQLLMQETFSR     AERDKLFQIIESRVVECQ+IEG+AAGRLTE+
Sbjct: 121 DFPPEKKDSKHLIEQLLMQETFSR-----AERDKLFQIIESRVVECQSIEGEAAGRLTEL 180

Query: 181 TSREVDDDDDSPAVCSTAILEAKKWLNEKRLGLGSSSTLELDHGTCTLNSTMLPVVNDEE 240
           +++ VD DD   AVCSTAILEAK WLNEKRLGLGSSSTLELDHG CTLNSTMLP+VNDEE
Sbjct: 181 SNKAVDSDDGGSAVCSTAILEAKTWLNEKRLGLGSSSTLELDHGPCTLNSTMLPLVNDEE 240

Query: 241 MGSPVDVAKSYMRARPPWASPSTNNFEFKSPSPSGLQLFKEETSYSISGNPLSSSKIKRE 300
            GS VDVAKSYMR RP WASPS NNFEFKSPSPS LQLFKEETS+S+SGNPLSSSKIKRE
Sbjct: 241 TGSLVDVAKSYMRERPIWASPSVNNFEFKSPSPSRLQLFKEETSFSVSGNPLSSSKIKRE 300

Query: 301 SPAGGSWNIQEEIRRVRSRATEEMLKTPSS-KLDWSSFASDYRSNLNSTRSDYLKIYSED 360
           SPA GSWNIQEEIRRVRS+ATEEML++ SS KLDWS FASDY+ NL+S RSDY KI+S++
Sbjct: 301 SPASGSWNIQEEIRRVRSKATEEMLRSRSSAKLDWSLFASDYKGNLSSARSDYPKIHSQE 360

Query: 361 KMQHAVKSIDKSMNWSADNTVTHTLSESKITQDVSENGACLLDTISPIQQQDKDLETNPT 420
           KMQHAV+SIDKSMN  AD +VTH LSESKITQDV ENGACLL + S  QQQDKD ETNPT
Sbjct: 361 KMQHAVQSIDKSMNRFADISVTHNLSESKITQDVPENGACLLGSGSIFQQQDKDFETNPT 420

Query: 421 TERKASNSSLDERECSTAHEVTRLANGFPSVPSS-GELAIEQNHVEKIVEENDSSGHNNE 480
            ERKASNSSLDER CST HEV  LANG PS+PSS GELA+E+NHV+KIVEE++SSGH++E
Sbjct: 421 NERKASNSSLDERGCSTVHEVAGLANGLPSLPSSTGELAVEENHVDKIVEESNSSGHDHE 480

Query: 481 AKGLALEERCELLSEVSMEVPNVNEND--TDKVLSDGNDVGKVASEGNDNGASKEKDGAG 537
           AKGL ++ERCE LSEVSMEVPN N+ D   +KV SDGN VGK  SEGN NG SKEK+ AG
Sbjct: 481 AKGLPVKERCE-LSEVSMEVPNTNDADATANKVPSDGNQVGKDVSEGNHNG-SKEKNDAG 532

BLAST of Spg016728 vs. ExPASy TrEMBL
Match: A0A6J1CFJ1 (protein KAKU4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010755 PE=4 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 1.1e-221
Identity = 429/560 (76.61%), Postives = 470/560 (83.93%), Query Frame = 0

Query: 1   MASLPAYREAAGSRSGGKIVRARRVRSRNHPYERPGLSKLGPGGNPSWLSRFIFSPTLTI 60
           MA++P+YR+  GSRSGGKIVRARR RSR +PYERPGLS LG GGNPSW+SRFIFSPTLTI
Sbjct: 1   MAAVPSYRDITGSRSGGKIVRARRARSRKNPYERPGLSNLGAGGNPSWISRFIFSPTLTI 60

Query: 61  ATGAGKLLSSVFVSDSSSSSSDSDSEDGVEDDVHDENRVFQGTEGVKKNGKSEMVNLFRK 120
           A+GAGKLLSSVFVSDS SSSSD DSED VE D HDEN  FQG EGVKKNG SE V+LFRK
Sbjct: 61  ASGAGKLLSSVFVSDSDSSSSDGDSEDDVEVDDHDENHFFQGAEGVKKNGTSETVDLFRK 120

Query: 121 DFPPEKKDSKHLIEQLLMQETFSRYDMIKAERDKLFQIIESRVVECQTIEGDAAGRLTEI 180
           DFPP+KKDSKHLIEQLLMQETFSR     AERDKLFQIIESRVVE QTI+ +AAGRLTEI
Sbjct: 121 DFPPQKKDSKHLIEQLLMQETFSR-----AERDKLFQIIESRVVEFQTIDAEAAGRLTEI 180

Query: 181 TSREVDDDDDSPAVCSTAILEAKKWLNEKRLGLGSSSTLELDHGTCTLNSTMLPVVNDEE 240
           ++R VD D+D PAVCSTAILEAKKWLNEKRLGLGSSST ++DHG CTLNSTML V NDEE
Sbjct: 181 SNRAVDSDNDRPAVCSTAILEAKKWLNEKRLGLGSSSTSDMDHGPCTLNSTMLTVANDEE 240

Query: 241 MGSPVDVAKSYMRARPPWASPSTNNFEFKSPSPSGLQLFKEETSYSISGNPLSSSKIKRE 300
           MGSPVDVAKSYMRARPPWASPS+NNFEFKSPSP GLQLFKEETSYSI+GNPLSSSK+KRE
Sbjct: 241 MGSPVDVAKSYMRARPPWASPSSNNFEFKSPSPLGLQLFKEETSYSITGNPLSSSKMKRE 300

Query: 301 SPAGGSWNIQEEIRRVRSRATEEMLKT-PSSKLDWSSFASDYRSNLNSTRSDYLKIYSED 360
           SPA GSWNIQEEIRRVRSRATEEML+T PS+KLDWSS  SDY+SNL+S  SD  KI+SED
Sbjct: 301 SPASGSWNIQEEIRRVRSRATEEMLRTLPSAKLDWSSLTSDYKSNLSSVHSDNPKIHSED 360

Query: 361 KMQHAVKSIDKSMNWSADNTVTHTLSESKITQDVSENGACLLDTISPIQQQDKDLETNPT 420
           KMQHA K IDK + WSADNT TH LSESKITQDVSENGACLLDT + I QQDKDL+TNPT
Sbjct: 361 KMQHAAKLIDKPLYWSADNTATHNLSESKITQDVSENGACLLDTTTIIVQQDKDLQTNPT 420

Query: 421 TERKASNSSLDERECSTAHEVTRLANGFPSVP-SSGELAIEQNHVEKIVEENDSSGHNNE 480
           TERK SNSSLDER+CST HEV  LANGF  VP SSGEL ++     KIVEEN+SSGH++E
Sbjct: 421 TERKVSNSSLDERDCSTVHEVAGLANGFSPVPSSSGELVVD----NKIVEENNSSGHDHE 480

Query: 481 AKGLALEERCELLSEVSMEVPNVNENDTDKVLSDGNDVGKVASEGNDNGASKEKDGAGNA 540
           AKG  +EERCELLSE SMEVPNV E  TDKV S+GND+GK+  E N   ASKEK   G  
Sbjct: 481 AKGPPVEERCELLSEASMEVPNVVEY-TDKVPSEGNDIGKILPEENGATASKEKTDGGK- 540

Query: 541 VFEHAHCVARNALDFSSSSG 559
                + V + +L  S+S G
Sbjct: 541 ----VNNVEKPSLQSSASVG 545

BLAST of Spg016728 vs. ExPASy TrEMBL
Match: A0A6J1CEG9 (protein KAKU4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010755 PE=4 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 2.5e-221
Identity = 428/560 (76.43%), Postives = 470/560 (83.93%), Query Frame = 0

Query: 1   MASLPAYREAAGSRSGGKIVRARRVRSRNHPYERPGLSKLGPGGNPSWLSRFIFSPTLTI 60
           MA++P+YR+  GSRSGGKIVRARR RSR +PYERPGLS LG GGNPSW+SRFIFSPTLTI
Sbjct: 1   MAAVPSYRDITGSRSGGKIVRARRARSRKNPYERPGLSNLGAGGNPSWISRFIFSPTLTI 60

Query: 61  ATGAGKLLSSVFVSDSSSSSSDSDSEDGVEDDVHDENRVFQGTEGVKKNGKSEMVNLFRK 120
           A+GAGKLLSSVFVSDS SSSSD DSED VE D HDEN  FQG EGVKKNG SE V+LFRK
Sbjct: 61  ASGAGKLLSSVFVSDSDSSSSDGDSEDDVEVDDHDENHFFQGAEGVKKNGTSETVDLFRK 120

Query: 121 DFPPEKKDSKHLIEQLLMQETFSRYDMIKAERDKLFQIIESRVVECQTIEGDAAGRLTEI 180
           DFPP+KKDSKHLIEQLLMQETFS     +AERDKLFQIIESRVVE QTI+ +AAGRLTEI
Sbjct: 121 DFPPQKKDSKHLIEQLLMQETFSS----RAERDKLFQIIESRVVEFQTIDAEAAGRLTEI 180

Query: 181 TSREVDDDDDSPAVCSTAILEAKKWLNEKRLGLGSSSTLELDHGTCTLNSTMLPVVNDEE 240
           ++R VD D+D PAVCSTAILEAKKWLNEKRLGLGSSST ++DHG CTLNSTML V NDEE
Sbjct: 181 SNRAVDSDNDRPAVCSTAILEAKKWLNEKRLGLGSSSTSDMDHGPCTLNSTMLTVANDEE 240

Query: 241 MGSPVDVAKSYMRARPPWASPSTNNFEFKSPSPSGLQLFKEETSYSISGNPLSSSKIKRE 300
           MGSPVDVAKSYMRARPPWASPS+NNFEFKSPSP GLQLFKEETSYSI+GNPLSSSK+KRE
Sbjct: 241 MGSPVDVAKSYMRARPPWASPSSNNFEFKSPSPLGLQLFKEETSYSITGNPLSSSKMKRE 300

Query: 301 SPAGGSWNIQEEIRRVRSRATEEMLKT-PSSKLDWSSFASDYRSNLNSTRSDYLKIYSED 360
           SPA GSWNIQEEIRRVRSRATEEML+T PS+KLDWSS  SDY+SNL+S  SD  KI+SED
Sbjct: 301 SPASGSWNIQEEIRRVRSRATEEMLRTLPSAKLDWSSLTSDYKSNLSSVHSDNPKIHSED 360

Query: 361 KMQHAVKSIDKSMNWSADNTVTHTLSESKITQDVSENGACLLDTISPIQQQDKDLETNPT 420
           KMQHA K IDK + WSADNT TH LSESKITQDVSENGACLLDT + I QQDKDL+TNPT
Sbjct: 361 KMQHAAKLIDKPLYWSADNTATHNLSESKITQDVSENGACLLDTTTIIVQQDKDLQTNPT 420

Query: 421 TERKASNSSLDERECSTAHEVTRLANGFPSVP-SSGELAIEQNHVEKIVEENDSSGHNNE 480
           TERK SNSSLDER+CST HEV  LANGF  VP SSGEL ++     KIVEEN+SSGH++E
Sbjct: 421 TERKVSNSSLDERDCSTVHEVAGLANGFSPVPSSSGELVVD----NKIVEENNSSGHDHE 480

Query: 481 AKGLALEERCELLSEVSMEVPNVNENDTDKVLSDGNDVGKVASEGNDNGASKEKDGAGNA 540
           AKG  +EERCELLSE SMEVPNV E  TDKV S+GND+GK+  E N   ASKEK   G  
Sbjct: 481 AKGPPVEERCELLSEASMEVPNVVEY-TDKVPSEGNDIGKILPEENGATASKEKTDGGK- 540

Query: 541 VFEHAHCVARNALDFSSSSG 559
                + V + +L  S+S G
Sbjct: 541 ----VNNVEKPSLQSSASVG 546

BLAST of Spg016728 vs. ExPASy TrEMBL
Match: A0A6J1FM93 (protein KAKU4-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446684 PE=4 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 2.1e-220
Identity = 430/544 (79.04%), Postives = 472/544 (86.76%), Query Frame = 0

Query: 1   MASLPAYREAAGSRSGGKIVRARRVRSRNHPYERPGLSKLGPGGNPSWLSRFIFSPTLTI 60
           MAS+PAYREA GSR+GGKIVRA+R  SR  PYERPGLS LGPGGNPSW+SRFIFSPTLTI
Sbjct: 1   MASVPAYREAGGSRTGGKIVRAKRAWSRKTPYERPGLSNLGPGGNPSWISRFIFSPTLTI 60

Query: 61  ATGAGKLLSSVFVSDSSSSSSDSDSEDGVEDDVHDENRVFQGTEGVKKNGKSEMVNLFRK 120
           A+GAGK LSSVF+S+SSSSSSDSDSED VEDDVHD+NR FQG  GVKK+G +EMVN FR 
Sbjct: 61  ASGAGKFLSSVFISESSSSSSDSDSEDDVEDDVHDDNRDFQG-NGVKKSGTTEMVNSFR- 120

Query: 121 DFPPEKKDSKHLIEQLLMQETFSRYDMIKAERDKLFQIIESRVVECQTIEGDAAGRLTEI 180
           DFPPEKKD K LIEQLLMQETFSR     AERDKLFQIIESRVVECQ+IEG+AAGRLTE+
Sbjct: 121 DFPPEKKDGKRLIEQLLMQETFSR-----AERDKLFQIIESRVVECQSIEGEAAGRLTEL 180

Query: 181 TSREVDDDDDSPAVCSTAILEAKKWLNEKRLGLGSSSTLELDHGTCTLNSTMLPVVNDEE 240
           +++ VD DD   AVCSTAILEAK WLNEKRLGLGSSS LELDHG CTLNSTMLPVVNDEE
Sbjct: 181 SNKAVDSDDGGSAVCSTAILEAKAWLNEKRLGLGSSSPLELDHGPCTLNSTMLPVVNDEE 240

Query: 241 MGSPVDVAKSYMRARPPWASPSTNNFEFKSPSPSGLQLFKEETSYSISGNPLSSSKIKRE 300
            GS VDVAKSYMR RP WASPS NNFEFKSPSPS L+LFKEETS+S+SGNPLSSSKIKRE
Sbjct: 241 TGSLVDVAKSYMRERPLWASPSANNFEFKSPSPSRLRLFKEETSFSVSGNPLSSSKIKRE 300

Query: 301 SPAGGSWNIQEEIRRVRSRATEEMLKTPSS-KLDWSSFASDYRSNLNSTRSDYLKIYSED 360
           SPA GSWNIQEEIRRVRS+ATEEML++ SS KLDWSSFASDY+ NL+S RSDY KI+SED
Sbjct: 301 SPASGSWNIQEEIRRVRSKATEEMLRSRSSAKLDWSSFASDYKGNLSSARSDYPKIHSED 360

Query: 361 KMQHAVKSIDKSMNWSADNTVTHTLSESKITQDVSENGACLLDTISPIQQQDKDLETNPT 420
           K QHAV+SIDKS+NWSAD +VTH LSESKITQD+ ENGACLL + S IQQQDKD ETNPT
Sbjct: 361 KTQHAVQSIDKSINWSADISVTHDLSESKITQDMPENGACLLGSASIIQQQDKDFETNPT 420

Query: 421 TERKASNSSLDERECSTAHEVTRLANGFPSVPSS-GELAIEQNHVE-KIVEENDSSGHNN 480
            ERKASNSSLDER CST HEV  LANG PS+PSS GELA+E+NHV+ KI EE++SSGH++
Sbjct: 421 NERKASNSSLDERGCSTVHEVAGLANGLPSLPSSTGELAVEENHVDKKIAEESNSSGHDH 480

Query: 481 EAKGLALEERCELLSEVSMEVPNVNEND--TDKVLSDGNDVGKVASEGNDNGASKEKDGA 540
           EAKGLA++ERCE LSEVSMEVPN N+ D   DKV SDGN VGK  SEGN NG SKEK+ A
Sbjct: 481 EAKGLAVKERCE-LSEVSMEVPNANDADATADKVPSDGNHVGKDVSEGNHNG-SKEKNDA 535

BLAST of Spg016728 vs. ExPASy TrEMBL
Match: A0A6J1J3P7 (protein KAKU4-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111480997 PE=4 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 2.4e-219
Identity = 427/540 (79.07%), Postives = 470/540 (87.04%), Query Frame = 0

Query: 1   MASLPAYREAAGSRSGGKIVRARRVRSRNHPYERPGLSKLGPGGNPSWLSRFIFSPTLTI 60
           MAS+PAYREA GSR+GGKIVRARR  SR  PYERPGLS LGPGGNPSW+SRFIFSPTLTI
Sbjct: 1   MASVPAYREAGGSRTGGKIVRARRAWSRKTPYERPGLSNLGPGGNPSWISRFIFSPTLTI 60

Query: 61  ATGAGKLLSSVFVSDSSSSSSDSDSEDGVEDDVHDENRVFQGTEGVKKNGKSEMVNLFRK 120
           A+GAGK LSSVF+++SSSSSSDSDSED VEDDVHD+NRVFQG  GVKK+G SEMVN FRK
Sbjct: 61  ASGAGKFLSSVFITESSSSSSDSDSEDDVEDDVHDDNRVFQG-NGVKKSGTSEMVNSFRK 120

Query: 121 DFPPEKKDSKHLIEQLLMQETFSRYDMIKAERDKLFQIIESRVVECQTIEGDAAGRLTEI 180
           DFPPEKKDSKHLIEQLLMQETFSR     AERDKLFQIIESRVVECQ+IEG+AAGRLTE+
Sbjct: 121 DFPPEKKDSKHLIEQLLMQETFSR-----AERDKLFQIIESRVVECQSIEGEAAGRLTEL 180

Query: 181 TSREVDDDDDSPAVCSTAILEAKKWLNEKRLGLGSSSTLELDHGTCTLNSTMLPVVNDEE 240
           +++ VD      +VCSTAILEAK WLNEKRLGLGSSSTLELDHG CTLNSTMLP+VNDEE
Sbjct: 181 SNKAVD------SVCSTAILEAKTWLNEKRLGLGSSSTLELDHGPCTLNSTMLPLVNDEE 240

Query: 241 MGSPVDVAKSYMRARPPWASPSTNNFEFKSPSPSGLQLFKEETSYSISGNPLSSSKIKRE 300
            GS VDVAKSYMR RP WASPS NNFEFKSPSPS LQLFKEETS+S+SGNPLSSSKIKRE
Sbjct: 241 TGSLVDVAKSYMRERPIWASPSVNNFEFKSPSPSRLQLFKEETSFSVSGNPLSSSKIKRE 300

Query: 301 SPAGGSWNIQEEIRRVRSRATEEMLKTPSS-KLDWSSFASDYRSNLNSTRSDYLKIYSED 360
           SPA GSWNIQEEIRRVRS+ATEEML++ SS KLDWS FASDY+ NL+S RSDY KI+S++
Sbjct: 301 SPASGSWNIQEEIRRVRSKATEEMLRSRSSAKLDWSLFASDYKGNLSSARSDYPKIHSQE 360

Query: 361 KMQHAVKSIDKSMNWSADNTVTHTLSESKITQDVSENGACLLDTISPIQQQDKDLETNPT 420
           KMQHAV+SIDKSMN  AD +VTH LSESKITQDV ENGACLL + S  QQQDKD ETNPT
Sbjct: 361 KMQHAVQSIDKSMNRFADISVTHNLSESKITQDVPENGACLLGSGSIFQQQDKDFETNPT 420

Query: 421 TERKASNSSLDERECSTAHEVTRLANGFPSVPSS-GELAIEQNHVEKIVEENDSSGHNNE 480
            ERKASNSSLDER CST HEV  LANG PS+PSS GELA+E+NHV+KIVEE++SSGH++E
Sbjct: 421 NERKASNSSLDERGCSTVHEVAGLANGLPSLPSSTGELAVEENHVDKIVEESNSSGHDHE 480

Query: 481 AKGLALEERCELLSEVSMEVPNVNEND--TDKVLSDGNDVGKVASEGNDNGASKEKDGAG 537
           AKGL ++ERCE LSEVSMEVPN N+ D   +KV SDGN VGK  SEGN NG SKEK+ AG
Sbjct: 481 AKGLPVKERCE-LSEVSMEVPNTNDADATANKVPSDGNQVGKDVSEGNHNG-SKEKNDAG 526

BLAST of Spg016728 vs. TAIR 10
Match: AT4G31430.1 (unknown protein; LOCATED IN: plasma membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 189.9 bits (481), Expect = 5.6e-48
Identity = 140/334 (41.92%), Postives = 188/334 (56.29%), Query Frame = 0

Query: 1   MASLPAYREAAGS-RSGGKIVRARRVRSRNHPYERP-GLSKLGPGGNPSWLSRFIFSPTL 60
           M S+  Y   AG  R GGKIVR RR      P ERP   S+  P  NPSW+SR ++ P  
Sbjct: 1   MDSVSGYAAGAGQPRVGGKIVRPRRTAVVRTPCERPVQRSRDPPQQNPSWISRLVYKPAS 60

Query: 61  TIATGAGKLLSSVFVSDSSSSSSDSDSEDGVEDDVHDENRVFQGTEGVKKNGK--SEMVN 120
            IA+GAGK +SSV  SDSSSSS + +          D +    G E V+KN    +E   
Sbjct: 61  VIASGAGKFISSVVFSDSSSSSEEDE----------DSSSDIDGDEDVEKNNPDFTEEDL 120

Query: 121 LFRKDFPPEKKDSKHLIEQLLMQETFSRYDMIKAERDKLFQIIESRVVECQTIEGDAAGR 180
           L  +    ++  SK +IEQLL+QETF+R      E D+L  II++RVV+  ++       
Sbjct: 121 LSAQQPSIQRLSSKRVIEQLLLQETFAR-----EEGDRLIDIIKARVVDHPSVPSAIE-- 180

Query: 181 LTEITSREVDDDDDSPAVCSTAILEAKKWLNEKRLGLGSSSTLELDHGTCTLNSTMLPVV 240
            T      +  D +   + +TA++EA+KWL EK+   GSSS  +                
Sbjct: 181 -TSHDDYGLTSDVNVGEMSNTAVMEARKWLEEKK--SGSSSKYK---------------A 240

Query: 241 NDEEMGSPVDVAKSYMRARPPWASPSTNNFEFKSPSPSGLQLFKEETSYSISGNPLSSSK 300
            ++  GSPVDVAKSYMRAR PW SP+ NN +F+SPS + +Q     T    S    SSSK
Sbjct: 241 TEDGAGSPVDVAKSYMRARLPWGSPAANNLDFRSPSSARVQ----GTPLPYSAGNFSSSK 295

Query: 301 IKRESPAGGSWNIQEEIRRVRSRATEEMLKTPSS 331
           +KR+S +  SWNIQ+EIR+VR++ATEEMLK+PSS
Sbjct: 301 LKRKSGSNQSWNIQDEIRKVRAKATEEMLKSPSS 295

BLAST of Spg016728 vs. TAIR 10
Match: AT4G31430.2 (unknown protein; LOCATED IN: plasma membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 189.9 bits (481), Expect = 5.6e-48
Identity = 140/334 (41.92%), Postives = 188/334 (56.29%), Query Frame = 0

Query: 1   MASLPAYREAAGS-RSGGKIVRARRVRSRNHPYERP-GLSKLGPGGNPSWLSRFIFSPTL 60
           M S+  Y   AG  R GGKIVR RR      P ERP   S+  P  NPSW+SR ++ P  
Sbjct: 1   MDSVSGYAAGAGQPRVGGKIVRPRRTAVVRTPCERPVQRSRDPPQQNPSWISRLVYKPAS 60

Query: 61  TIATGAGKLLSSVFVSDSSSSSSDSDSEDGVEDDVHDENRVFQGTEGVKKNGK--SEMVN 120
            IA+GAGK +SSV  SDSSSSS + +          D +    G E V+KN    +E   
Sbjct: 61  VIASGAGKFISSVVFSDSSSSSEEDE----------DSSSDIDGDEDVEKNNPDFTEEDL 120

Query: 121 LFRKDFPPEKKDSKHLIEQLLMQETFSRYDMIKAERDKLFQIIESRVVECQTIEGDAAGR 180
           L  +    ++  SK +IEQLL+QETF+R      E D+L  II++RVV+  ++       
Sbjct: 121 LSAQQPSIQRLSSKRVIEQLLLQETFAR-----EEGDRLIDIIKARVVDHPSVPSAIE-- 180

Query: 181 LTEITSREVDDDDDSPAVCSTAILEAKKWLNEKRLGLGSSSTLELDHGTCTLNSTMLPVV 240
            T      +  D +   + +TA++EA+KWL EK+   GSSS  +                
Sbjct: 181 -TSHDDYGLTSDVNVGEMSNTAVMEARKWLEEKK--SGSSSKYK---------------A 240

Query: 241 NDEEMGSPVDVAKSYMRARPPWASPSTNNFEFKSPSPSGLQLFKEETSYSISGNPLSSSK 300
            ++  GSPVDVAKSYMRAR PW SP+ NN +F+SPS + +Q     T    S    SSSK
Sbjct: 241 TEDGAGSPVDVAKSYMRARLPWGSPAANNLDFRSPSSARVQ----GTPLPYSAGNFSSSK 295

Query: 301 IKRESPAGGSWNIQEEIRRVRSRATEEMLKTPSS 331
           +KR+S +  SWNIQ+EIR+VR++ATEEMLK+PSS
Sbjct: 301 LKRKSGSNQSWNIQDEIRKVRAKATEEMLKSPSS 295

BLAST of Spg016728 vs. TAIR 10
Match: AT4G31430.3 (unknown protein; LOCATED IN: plasma membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 189.9 bits (481), Expect = 5.6e-48
Identity = 140/334 (41.92%), Postives = 188/334 (56.29%), Query Frame = 0

Query: 1   MASLPAYREAAGS-RSGGKIVRARRVRSRNHPYERP-GLSKLGPGGNPSWLSRFIFSPTL 60
           M S+  Y   AG  R GGKIVR RR      P ERP   S+  P  NPSW+SR ++ P  
Sbjct: 1   MDSVSGYAAGAGQPRVGGKIVRPRRTAVVRTPCERPVQRSRDPPQQNPSWISRLVYKPAS 60

Query: 61  TIATGAGKLLSSVFVSDSSSSSSDSDSEDGVEDDVHDENRVFQGTEGVKKNGK--SEMVN 120
            IA+GAGK +SSV  SDSSSSS + +          D +    G E V+KN    +E   
Sbjct: 61  VIASGAGKFISSVVFSDSSSSSEEDE----------DSSSDIDGDEDVEKNNPDFTEEDL 120

Query: 121 LFRKDFPPEKKDSKHLIEQLLMQETFSRYDMIKAERDKLFQIIESRVVECQTIEGDAAGR 180
           L  +    ++  SK +IEQLL+QETF+R      E D+L  II++RVV+  ++       
Sbjct: 121 LSAQQPSIQRLSSKRVIEQLLLQETFAR-----EEGDRLIDIIKARVVDHPSVPSAIE-- 180

Query: 181 LTEITSREVDDDDDSPAVCSTAILEAKKWLNEKRLGLGSSSTLELDHGTCTLNSTMLPVV 240
            T      +  D +   + +TA++EA+KWL EK+   GSSS  +                
Sbjct: 181 -TSHDDYGLTSDVNVGEMSNTAVMEARKWLEEKK--SGSSSKYK---------------A 240

Query: 241 NDEEMGSPVDVAKSYMRARPPWASPSTNNFEFKSPSPSGLQLFKEETSYSISGNPLSSSK 300
            ++  GSPVDVAKSYMRAR PW SP+ NN +F+SPS + +Q     T    S    SSSK
Sbjct: 241 TEDGAGSPVDVAKSYMRARLPWGSPAANNLDFRSPSSARVQ----GTPLPYSAGNFSSSK 295

Query: 301 IKRESPAGGSWNIQEEIRRVRSRATEEMLKTPSS 331
           +KR+S +  SWNIQ+EIR+VR++ATEEMLK+PSS
Sbjct: 301 LKRKSGSNQSWNIQDEIRKVRAKATEEMLKSPSS 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898993.15.0e-22479.44protein KAKU4 [Benincasa hispida][more]
XP_022982016.12.8e-22279.63protein KAKU4-like isoform X1 [Cucurbita maxima][more]
XP_022139992.12.3e-22176.61protein KAKU4 isoform X2 [Momordica charantia][more]
KAG6608200.14.0e-22179.41Protein KAKU4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022139984.15.2e-22176.43protein KAKU4 isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q949W67.9e-4741.92Protein KAKU4 OS=Arabidopsis thaliana OX=3702 GN=KAKU4 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IY541.3e-22279.63protein KAKU4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480997 PE=4 S... [more]
A0A6J1CFJ11.1e-22176.61protein KAKU4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010755 PE=4 SV=... [more]
A0A6J1CEG92.5e-22176.43protein KAKU4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010755 PE=4 SV=... [more]
A0A6J1FM932.1e-22079.04protein KAKU4-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446684 PE=4... [more]
A0A6J1J3P72.4e-21979.07protein KAKU4-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111480997 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT4G31430.15.6e-4841.92unknown protein; LOCATED IN: plasma membrane; EXPRESSED IN: 25 plant structures;... [more]
AT4G31430.25.6e-4841.92unknown protein; LOCATED IN: plasma membrane; EXPRESSED IN: 25 plant structures;... [more]
AT4G31430.35.6e-4841.92unknown protein; LOCATED IN: plasma membrane; EXPRESSED IN: 25 plant structures;... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 502..535
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 76..96
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 407..434
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..45
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 415..434
NoneNo IPR availablePANTHERPTHR33416FAMILY NOT NAMEDcoord: 1..510
NoneNo IPR availablePANTHERPTHR33416:SF17PROTEIN KAKU4coord: 1..510

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg016728.1Spg016728.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071763 nuclear membrane organization
cellular_component GO:0005635 nuclear envelope