Spg016673 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg016673
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	protein DCL homolog, chloroplastic
Location	scaffold9: 45757745 .. 45760480 (-)
RNA-Seq Expression	Spg016673
Synteny	Spg016673

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTGAAGAGGATGCGGATTGAAGAGAAATCCTGTAGAATCTCCAAATCCATGAGATTGATGATAAAGTGAGTGACCGTTAACAGTTCTTCTTGCTGCGTTTTCTCAGAGAATTGTAAGAAAAACCATGCTCGGCGATCGCAGGCTACCATTTCATTCTCAATAGTTGCAAATTTAAGCTCCTTGTTTCAATTGAACAAGGGCATGGTCATGGCCATGGCGTCCATTTTGAAGCCACCGCCATTTGTGCCCTTCCACTCCTTGAATCCAAGCTTCTTCAACTCTTCACCAGTGATCTTATGTTTTCCTACGCATCCAATGACGTCGTTTCATCCATCCACTCGTGCCCTAAAGACTGGCCCTGAAGGCAGTAGGATTCGGAGCCATGAAGAATATAGCTCCGATTTGCTGCGGAAACCGTTTGTTCCGCCGGCGAAGGACTTGGCTGGAATATCGGAGGATGACGAGAGCAGCGAGGAGAGTGGAAATGACGACCAGGAGGAAGAGGATTGGGTTGATTGGGAGGACAAGATTTTGGAGGATACTGTTCCTCTAGTTGGCTTTGTTAGGATGCTTCTTCACTCTGGAAAGTAAGCAATCGTTCGATTCTGTGTCGGCTTTATAACTGAATTTGAGCTTGTTCCTTGTCAAATAGAAATTATGCTACCTTGCTGTCAAGGTGAATTTTGAACTTGTTTTTGCGCAAATGGAAAACGTTTACTCGAAACTGAACGGACCCATATAGCTTGAGATTGAAATTGTAGAATTTTGACGCAATTTCTATAGCAAGCAAGAACAAGAATTATGAGTCTTGTTAATTAATCTCTTCAATTTCCATTGATTTGACAATCAATTTGTTCGAATTTAAGTATAACGCGATGTCCCTAAAGATTTTGAATCTAATAAATTGAAAAAAAAAGGGCTCTTGCAACAGTACACACTCAACCTCTGGCCCTTAGCCCATGAATATTACTTGAATTACTTGCAATATGATCTGAATGGAAGGGGGTTTCACAATATGAATGATTAAGATAAGTCCTATATCAATATGTAGGATAAATCAATTGTTTACTCAATACATCCTCGTACTGTTCGTATATTTCAACCATCAGTTGTTTGTATATATGCTGTTATCTTCCGGTATGAGTTCCCAGTTGCTATCATTTCAGCATATCTCCTTAAAGACAAGGTTTTAACATTTTTTGGTTGTTTTTCTTCCCTAGGATTTATGAGGTATCTTTACTGTGTGCTTTGATCATGTAAATGTCAACTAATGGAGCACCTATTATTTGAAAGAGAATATATTCTTACTATCTTCTTGAATTCGTTAGCATGATGATGTAGAGTTTAATCTTCATTCATTGATCATCTTTAATCAAACATATGCAGATATGGAAGTGGGGATAGGTTAAGCCCTGAACACGAGAAAACAATTCTTGACCGGTTGCTTCCCTATCATCCCGAGTGTGAGAAGAAGATTGGATGTGGGATCAATTATATCACGGTATATCTCTCTCCATATTTGTTCTTGCCTCATGTTTTTAGCTTATTTTTAGAATTTACATTTACATTAAATATAATAACTAAATAGATACTCTAGCCTCTTTTCTATTTCCTTGGATGAAAGAAAGTTGTTGATTACTTCTGATGAAACATCCTGGTTATAGCCATTGATTTCAATCACTTATGATTTCTAGAACATTTGACTAGCTTGTAATCCTAAAATATTTGTCTATAATGCTGCACTTGGAAAGATACAAATTGTTGTGGAACAGAACAAGTATTAGAAAGGAAAAGTTAATTTTTCCTTCTACCCGTGATCTATATTAACTGTGTGGATTTTTTGTAAAAAACTTTTGCCTGTGATTTAAATGAAGACACTTCAAGTAATTATCGATGTGCATAGTGAGAAACATTGGCATTGATAAGTGGCAATGGTTCGCTTTGTTGGAACATTGTATTACTCGATTGTATATTTGTGATGTTGCCAAAAGTTATACCTGGACTTGAACTCCAATATCTTGACATGAGAGAAGTGATTTTATGTCTCTTATTATGGGAAATAGGAAAAAGAAGAAAAACCTGCTCATGGGCAGTGCCATTTTCACTGCTGAACCATTTTTTTTTTTAATCTTTTTTGAGCATTGGATACATATTCTGTCTCTGTTAACTGAAATGATCGTAGCAATTATGTATTGGTGGTGTTGTGGTCGGCAGATTGGATATCATCCTGATTTTGAAAGCTCAAGATGTTTATTCATAGTCAGAAAAGACGGAGAGTTAGTTGATTTTTCATATTGGAAGTGCATCAAAGGTCTGATCAGAAAGAACTATCCTCTTCATGCAGAAAGCTTCATTCTTAGGCATTTTCGACGACGTAGACGCAATTTCCGTCGGTAACACATGCAAGATCTGTTAATCATACTACTGTACCATTCAGTTTCAGGAATTCTTTGAATATCAAAGCTTGAAATTGACGTACCACGCTTGTGTGGTTTTCTTGTGTTTAATGATTTGAAATGTGCGAATCAAGATGGATATAGGGTGAATGTAGACTCTTTACTTGAAGGAAGGTTCTAGTAAAATTCAATTTTGTAAATCTTAACAATTGCCTCTAGTTTTCCATGATCCAATTCGTGCTTAATGTCTAGTGTGGTTAAACTGGTTAGCTCTCGCACTTCCCAGTTATTCCCACCACCTACACTCTCTCGCTCACTCTAGCAGGAATATATGCTCGA

mRNA sequence

ATGGTCATGGCCATGGCGTCCATTTTGAAGCCACCGCCATTTGTGCCCTTCCACTCCTTGAATCCAAGCTTCTTCAACTCTTCACCAGTGATCTTATGTTTTCCTACGCATCCAATGACGTCGTTTCATCCATCCACTCGTGCCCTAAAGACTGGCCCTGAAGGCAGTAGGATTCGGAGCCATGAAGAATATAGCTCCGATTTGCTGCGGAAACCGTTTGTTCCGCCGGCGAAGGACTTGGCTGGAATATCGGAGGATGACGAGAGCAGCGAGGAGAGTGGAAATGACGACCAGGAGGAAGAGGATTGGGTTGATTGGGAGGACAAGATTTTGGAGGATACTGTTCCTCTAGTTGGCTTTGTTAGGATGCTTCTTCACTCTGGAAAATATGGAAGTGGGGATAGGTTAAGCCCTGAACACGAGAAAACAATTCTTGACCGGTTGCTTCCCTATCATCCCGAGTGTGAGAAGAAGATTGGATGTGGGATCAATTATATCACGATTGGATATCATCCTGATTTTGAAAGCTCAAGATGTTTATTCATAGTCAGAAAAGACGGAGAGTTAGTTGATTTTTCATATTGGAAGTGCATCAAAGGTCTGATCAGAAAGAACTATCCTCTTCATGCAGAAAGCTTCATTCTTAGGCATTTTCGACGACGTAGACGCAATTTCCGTCGGTAA

Coding sequence (CDS)

ATGGTCATGGCCATGGCGTCCATTTTGAAGCCACCGCCATTTGTGCCCTTCCACTCCTTGAATCCAAGCTTCTTCAACTCTTCACCAGTGATCTTATGTTTTCCTACGCATCCAATGACGTCGTTTCATCCATCCACTCGTGCCCTAAAGACTGGCCCTGAAGGCAGTAGGATTCGGAGCCATGAAGAATATAGCTCCGATTTGCTGCGGAAACCGTTTGTTCCGCCGGCGAAGGACTTGGCTGGAATATCGGAGGATGACGAGAGCAGCGAGGAGAGTGGAAATGACGACCAGGAGGAAGAGGATTGGGTTGATTGGGAGGACAAGATTTTGGAGGATACTGTTCCTCTAGTTGGCTTTGTTAGGATGCTTCTTCACTCTGGAAAATATGGAAGTGGGGATAGGTTAAGCCCTGAACACGAGAAAACAATTCTTGACCGGTTGCTTCCCTATCATCCCGAGTGTGAGAAGAAGATTGGATGTGGGATCAATTATATCACGATTGGATATCATCCTGATTTTGAAAGCTCAAGATGTTTATTCATAGTCAGAAAAGACGGAGAGTTAGTTGATTTTTCATATTGGAAGTGCATCAAAGGTCTGATCAGAAAGAACTATCCTCTTCATGCAGAAAGCTTCATTCTTAGGCATTTTCGACGACGTAGACGCAATTTCCGTCGGTAA

Protein sequence

MVMAMASILKPPPFVPFHSLNPSFFNSSPVILCFPTHPMTSFHPSTRALKTGPEGSRIRSHEEYSSDLLRKPFVPPAKDLAGISEDDESSEESGNDDQEEEDWVDWEDKILEDTVPLVGFVRMLLHSGKYGSGDRLSPEHEKTILDRLLPYHPECEKKIGCGINYITIGYHPDFESSRCLFIVRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNFRR

Homology

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BLAST of Spg016673 vs. NCBI nr
Match: XP_038897137.1 (protein DCL homolog, chloroplastic [Benincasa hispida])

HSP 1 Score: 438.0 bits (1125), Expect = 5.1e-119
Identity = 211/227 (92.95%), Postives = 220/227 (96.92%), Query Frame = 0


Query: 1   MVMAMASILKPPPFVPFHSLNPSFFNSSPVILCFPTHPMTSFHPSTRALKTGPEGSRIRS 60
           M+MAMASILKPPPFVP HSLNP+FFNSSPVILCFPT PMTSFH STRALKTGPEGSRIRS
Sbjct: 1   MLMAMASILKPPPFVPSHSLNPNFFNSSPVILCFPTQPMTSFHLSTRALKTGPEGSRIRS 60

Query: 61  HEEYSSDLLRKPFVPPAKDLAGISEDDESSEESGNDDQEEEDWVDWEDKILEDTVPLVGF 120
           HEEYSSDLLRKP VPPA+DLA +SEDD+SSEESGNDD+EEEDWVDWEDKILEDTVPLVGF
Sbjct: 61  HEEYSSDLLRKPVVPPARDLARLSEDDDSSEESGNDDEEEEDWVDWEDKILEDTVPLVGF 120

Query: 121 VRMLLHSGKYGSGDRLSPEHEKTILDRLLPYHPECEKKIGCGINYITIGYHPDFESSRCL 180
           VRM+LHSGKYGSGDRL+PEHEKTILDRLLPYHPE EKKIGCGI+YITIGYHPDFESSRCL
Sbjct: 121 VRMVLHSGKYGSGDRLNPEHEKTILDRLLPYHPESEKKIGCGIDYITIGYHPDFESSRCL 180

Query: 181 FIVRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNFRR 228
           FIVRKDGELVDFSYWKCIKGLIRK YPLHAESFILRHFRRRRRNFRR
Sbjct: 181 FIVRKDGELVDFSYWKCIKGLIRKKYPLHAESFILRHFRRRRRNFRR 227

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BLAST of Spg016673 vs. NCBI nr
Match: XP_022133006.1 (protein DCL homolog, chloroplastic [Momordica charantia])

HSP 1 Score: 429.1 bits (1102), Expect = 2.4e-116
Identity = 206/224 (91.96%), Postives = 211/224 (94.20%), Query Frame = 0


Query: 3   MAMASILKPPPFVPFHSLNPSFFNSSPVILCFPTHPMTSFHPSTRALKTGPEGSRIRSHE 62
           MAMAS+ KPPPF PFHSLNPSF NSSPVILCFP HPMTS HPS RALKTGPEGSRIRSHE
Sbjct: 1   MAMASVSKPPPFPPFHSLNPSFLNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIRSHE 60

Query: 63  EYSSDLLRKPFVPPAKDLAGISEDDESSEESGNDDQEEEDWVDWEDKILEDTVPLVGFVR 122
           EYSSDLLRKP VPPAKD A ISEDDE SEESG D +EEEDWVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVGFVR 120

Query: 123 MLLHSGKYGSGDRLSPEHEKTILDRLLPYHPECEKKIGCGINYITIGYHPDFESSRCLFI 182
           M+LHSGKYGSGDRLS EHEKTILDRLLPYHPECEKKIGCGI+YITIGYHPDFESSRCLFI
Sbjct: 121 MVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFI 180

Query: 183 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNFR 227
           VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRN+R
Sbjct: 181 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNYR 224

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BLAST of Spg016673 vs. NCBI nr
Match: XP_008452556.1 (PREDICTED: protein DCL, chloroplastic [Cucumis melo])

HSP 1 Score: 416.4 bits (1069), Expect = 1.6e-112
Identity = 199/225 (88.44%), Postives = 213/225 (94.67%), Query Frame = 0


Query: 3   MAMASILKPPPFVPFHSLNPSFFNSSPVILCFPTHPMTSFHPSTRALKTGPEGSRIRSHE 62
           MAMASIL   PF PFHSLNP+FFNSSP+ILCFPTHP+ SFHPSTRALKTGPEGSRIRSH+
Sbjct: 1   MAMASILTLLPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGSRIRSHQ 60

Query: 63  EYSSDLLRKPFVPPAKDLAGISEDDESSEESGNDDQEEEDWVDWEDKILEDTVPLVGFVR 122
           EYSSDLLRKP VPPAKDLAG SEDD+SSEESGND++EE DWVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVVPPAKDLAGPSEDDDSSEESGNDEEEEVDWVDWEDKILEDTVPLVGFVR 120

Query: 123 MLLHSGKYGSGDRLSPEHEKTILDRLLPYHPECEKKIGCGINYITIGYHPDFESSRCLFI 182
           M+LH+GKY SGDRL PEHEKTIL+RLLPYHPE EKKIGCG++YIT+GYHPDFESSRCLFI
Sbjct: 121 MVLHTGKYESGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 183 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNFRR 228
           VRKDGELVDFSYWKCIKGLIRKNYPL+AESFILRHFRRRRRN RR
Sbjct: 181 VRKDGELVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRNLRR 225

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BLAST of Spg016673 vs. NCBI nr
Match: KAA0064386.1 (protein DCL [Cucumis melo var. makuwa] >TYK20201.1 protein DCL [Cucumis melo var. makuwa])

HSP 1 Score: 416.4 bits (1069), Expect = 1.6e-112
Identity = 199/225 (88.44%), Postives = 213/225 (94.67%), Query Frame = 0


Query: 3   MAMASILKPPPFVPFHSLNPSFFNSSPVILCFPTHPMTSFHPSTRALKTGPEGSRIRSHE 62
           MAMASIL   PF PFHSLNP+FFNSSP+ILCFPTHP+ SFHPSTRALKTGPEGSRIRSH+
Sbjct: 1   MAMASILTLLPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGSRIRSHQ 60

Query: 63  EYSSDLLRKPFVPPAKDLAGISEDDESSEESGNDDQEEEDWVDWEDKILEDTVPLVGFVR 122
           EYSSDLLRKP VPPAKDLAG SEDD+SSEESGND++EE DWVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVVPPAKDLAGPSEDDDSSEESGNDEEEEVDWVDWEDKILEDTVPLVGFVR 120

Query: 123 MLLHSGKYGSGDRLSPEHEKTILDRLLPYHPECEKKIGCGINYITIGYHPDFESSRCLFI 182
           M+LH+GKY SGDRL PEHEKTIL+RLLPYHPE EKKIGCG++YIT+GYHPDFESSRCLFI
Sbjct: 121 MVLHTGKYESGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 183 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNFRR 228
           VRKDGELVDFSYWKCIKGLIRKNYPL+AESFILRHFRRRRRN RR
Sbjct: 181 VRKDGELVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRNLRR 225

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BLAST of Spg016673 vs. NCBI nr
Match: XP_004141259.1 (protein DCL homolog, chloroplastic [Cucumis sativus] >KGN55194.1 hypothetical protein Csa_011993 [Cucumis sativus])

HSP 1 Score: 413.3 bits (1061), Expect = 1.4e-111
Identity = 195/225 (86.67%), Postives = 213/225 (94.67%), Query Frame = 0


Query: 3   MAMASILKPPPFVPFHSLNPSFFNSSPVILCFPTHPMTSFHPSTRALKTGPEGSRIRSHE 62
           MAMASILKPPPF PFHSLNP+FFNSSP+ILCFPTHP+ SFHPSTRALKTGPEG R+RSH+
Sbjct: 1   MAMASILKPPPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIRLRSHQ 60

Query: 63  EYSSDLLRKPFVPPAKDLAGISEDDESSEESGNDDQEEEDWVDWEDKILEDTVPLVGFVR 122
           EYSSDLLRKP  P AKDLAG SEDD+SSEESGN+D+EE +WVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVR 120

Query: 123 MLLHSGKYGSGDRLSPEHEKTILDRLLPYHPECEKKIGCGINYITIGYHPDFESSRCLFI 182
           M+LH+GKY +GDRL PEHEKTIL+RLLPYHPE EKKIGCG++YIT+GYHPDFESSRCLFI
Sbjct: 121 MVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 183 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNFRR 228
           VRKDGE+VDFSYWKCIKGLIRKNYPL+AESFILRHFRRRRRN RR
Sbjct: 181 VRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRNSRR 225

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BLAST of Spg016673 vs. ExPASy Swiss-Prot
Match: Q9C642 (Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 2.4e-71
Identity = 128/200 (64.00%), Postives = 158/200 (79.00%), Query Frame = 0


Query: 25  FNSSPVILCFPTHPMTSFHPSTRALKTGPEGSRIRSHEEYSSDLLRKPFVPPAKDLAGIS 84
           F+SSP+ L FP    TS  P  RAL+T  +G++I + E Y S+LLR+P +          
Sbjct: 26  FSSSPLCLYFPRGDSTSLRPRVRALRTESDGAKIGNSESYGSELLRRPRI-------ASE 85

Query: 85  EDDESSEESGNDDQEEEDWVDWEDKILEDTVPLVGFVRMLLHSGKYGSGDRLSPEHEKTI 144
           E  E  EE   ++ E +++VDWEDKILE TVPLVGFVRM+LHSGKY + DRLSPEHE+TI
Sbjct: 86  ESSEEEEEEEEENSEGDEFVDWEDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTI 145

Query: 145 LDRLLPYHPECEKKIGCGINYITIGYHPDFESSRCLFIVRKDGELVDFSYWKCIKGLIRK 204
           ++ LLPYHPECEKKIGCGI+YI +G+HPDFESSRC+FIVRKDGE+VDFSYWKCIKGLI+K
Sbjct: 146 IEMLLPYHPECEKKIGCGIDYIMVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKK 205

Query: 205 NYPLHAESFILRHFRRRRRN 225
            YPL+A+SFILRHFR+RR+N
Sbjct: 206 KYPLYADSFILRHFRKRRQN 218

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BLAST of Spg016673 vs. ExPASy Swiss-Prot
Match: Q42463 (Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 2.5e-68
Identity = 125/180 (69.44%), Postives = 148/180 (82.22%), Query Frame = 0


Query: 48  ALKTGPEGSRIRSHEEYSSDLLRKPFVPPAKDLAGISED---DESSEESGNDDQEEEDWV 107
           A+KTG EG  IRS    +++LLRKP +    +    SE+   +ES +E G    + E WV
Sbjct: 47  AVKTGSEGGGIRSD---NAELLRKPVISTELETTSESEELVKEESDDEVGKKSGDGEGWV 106

Query: 108 DWEDKILEDTVPLVGFVRMLLHSGKYGSGDRLSPEHEKTILDRLLPYHPECEKKIGCGIN 167
           DWED+ILEDTVPLVGFVRM+LHSGKY  GDRLSP+H++TIL RLLPYHPEC+KKIG G++
Sbjct: 107 DWEDQILEDTVPLVGFVRMILHSGKYAIGDRLSPDHQRTILQRLLPYHPECDKKIGPGVD 166

Query: 168 YITIGYHPDFESSRCLFIVRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRN 225
           YIT+GYHPDFE+SRCLFIVRKDGE VDFSYWKCIKGLIRKNYPL+A+SFILRHFR+RRRN
Sbjct: 167 YITVGYHPDFENSRCLFIVRKDGETVDFSYWKCIKGLIRKNYPLYADSFILRHFRKRRRN 223

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BLAST of Spg016673 vs. ExPASy Swiss-Prot
Match: Q5D869 (DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPE1 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.6e-19
Identity = 45/130 (34.62%), Postives = 75/130 (57.69%), Query Frame = 0


Query: 98   QEEEDWVDWEDKILEDTVPLVGFVRMLLHSGKYGSGDRLSPEHEKTILDRLLPYHPECEK 157
            Q  + +   E ++L D  P++  +R ++H   Y  GD +S + +  +L+++L +HP+ E 
Sbjct: 1727 QRLDSFTSEEQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKET 1786

Query: 158  KIGCGINYITIGYHPDFESSRCLFIVRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRH 217
            K+G G+++IT+  H  F  SRC F+V  DG   DFSY K +   + K YP  AE FI ++
Sbjct: 1787 KLGSGVDFITVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKY 1846

Query: 218  FRRRRRNFRR 228
            F + R +  R
Sbjct: 1847 FTKPRPSGNR 1856

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BLAST of Spg016673 vs. ExPASy Swiss-Prot
Match: Q8L557 (Protein EMBRYO DEFECTIVE 514 OS=Arabidopsis thaliana OX=3702 GN=EMB514 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 7.9e-06
Identity = 25/59 (42.37%), Postives = 36/59 (61.02%), Query Frame = 0


Query: 144 ILDRLLPYHPECEKKIGCGINYITIGYHPDFESSRCLFIVRKDGELVDFSYWKCIKGLI 203
           +LD +   H E EKKIG GI    +  HP ++ SRC F+VR+D    DFS+ KC+  ++
Sbjct: 105 LLDLIKKGHSEPEKKIGGGIKTFQVRTHPMWK-SRCFFLVREDDTADDFSFRKCVDQIL 162

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BLAST of Spg016673 vs. ExPASy Swiss-Prot
Match: P85936 (Protein DCL, chloroplastic (Fragment) OS=Pseudotsuga menziesii OX=3357 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 7.4e-04
Identity = 18/22 (81.82%), Postives = 21/22 (95.45%), Query Frame = 0


Query: 103 WVDWEDKILEDTVPLVGFVRML 125
           WVDWED+IL+DTVPLV FVRM+
Sbjct: 2   WVDWEDQILQDTVPLVNFVRMI 23

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BLAST of Spg016673 vs. ExPASy TrEMBL
Match: A0A6J1BTU2 (protein DCL homolog, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111005680 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.2e-116
Identity = 206/224 (91.96%), Postives = 211/224 (94.20%), Query Frame = 0


Query: 3   MAMASILKPPPFVPFHSLNPSFFNSSPVILCFPTHPMTSFHPSTRALKTGPEGSRIRSHE 62
           MAMAS+ KPPPF PFHSLNPSF NSSPVILCFP HPMTS HPS RALKTGPEGSRIRSHE
Sbjct: 1   MAMASVSKPPPFPPFHSLNPSFLNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIRSHE 60

Query: 63  EYSSDLLRKPFVPPAKDLAGISEDDESSEESGNDDQEEEDWVDWEDKILEDTVPLVGFVR 122
           EYSSDLLRKP VPPAKD A ISEDDE SEESG D +EEEDWVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVGFVR 120

Query: 123 MLLHSGKYGSGDRLSPEHEKTILDRLLPYHPECEKKIGCGINYITIGYHPDFESSRCLFI 182
           M+LHSGKYGSGDRLS EHEKTILDRLLPYHPECEKKIGCGI+YITIGYHPDFESSRCLFI
Sbjct: 121 MVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFI 180

Query: 183 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNFR 227
           VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRN+R
Sbjct: 181 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNYR 224

[Close]

BLAST of Spg016673 vs. ExPASy TrEMBL
Match: A0A5A7VDC9 (Protein DCL OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003370 PE=4 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 7.7e-113
Identity = 199/225 (88.44%), Postives = 213/225 (94.67%), Query Frame = 0


Query: 3   MAMASILKPPPFVPFHSLNPSFFNSSPVILCFPTHPMTSFHPSTRALKTGPEGSRIRSHE 62
           MAMASIL   PF PFHSLNP+FFNSSP+ILCFPTHP+ SFHPSTRALKTGPEGSRIRSH+
Sbjct: 1   MAMASILTLLPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGSRIRSHQ 60

Query: 63  EYSSDLLRKPFVPPAKDLAGISEDDESSEESGNDDQEEEDWVDWEDKILEDTVPLVGFVR 122
           EYSSDLLRKP VPPAKDLAG SEDD+SSEESGND++EE DWVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVVPPAKDLAGPSEDDDSSEESGNDEEEEVDWVDWEDKILEDTVPLVGFVR 120

Query: 123 MLLHSGKYGSGDRLSPEHEKTILDRLLPYHPECEKKIGCGINYITIGYHPDFESSRCLFI 182
           M+LH+GKY SGDRL PEHEKTIL+RLLPYHPE EKKIGCG++YIT+GYHPDFESSRCLFI
Sbjct: 121 MVLHTGKYESGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 183 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNFRR 228
           VRKDGELVDFSYWKCIKGLIRKNYPL+AESFILRHFRRRRRN RR
Sbjct: 181 VRKDGELVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRNLRR 225

[Close]

BLAST of Spg016673 vs. ExPASy TrEMBL
Match: A0A1S3BVA4 (protein DCL, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493543 PE=4 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 7.7e-113
Identity = 199/225 (88.44%), Postives = 213/225 (94.67%), Query Frame = 0


Query: 3   MAMASILKPPPFVPFHSLNPSFFNSSPVILCFPTHPMTSFHPSTRALKTGPEGSRIRSHE 62
           MAMASIL   PF PFHSLNP+FFNSSP+ILCFPTHP+ SFHPSTRALKTGPEGSRIRSH+
Sbjct: 1   MAMASILTLLPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGSRIRSHQ 60

Query: 63  EYSSDLLRKPFVPPAKDLAGISEDDESSEESGNDDQEEEDWVDWEDKILEDTVPLVGFVR 122
           EYSSDLLRKP VPPAKDLAG SEDD+SSEESGND++EE DWVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVVPPAKDLAGPSEDDDSSEESGNDEEEEVDWVDWEDKILEDTVPLVGFVR 120

Query: 123 MLLHSGKYGSGDRLSPEHEKTILDRLLPYHPECEKKIGCGINYITIGYHPDFESSRCLFI 182
           M+LH+GKY SGDRL PEHEKTIL+RLLPYHPE EKKIGCG++YIT+GYHPDFESSRCLFI
Sbjct: 121 MVLHTGKYESGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 183 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNFRR 228
           VRKDGELVDFSYWKCIKGLIRKNYPL+AESFILRHFRRRRRN RR
Sbjct: 181 VRKDGELVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRNLRR 225

[Close]

BLAST of Spg016673 vs. ExPASy TrEMBL
Match: A0A0A0L2D0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639820 PE=4 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 6.5e-112
Identity = 195/225 (86.67%), Postives = 213/225 (94.67%), Query Frame = 0


Query: 3   MAMASILKPPPFVPFHSLNPSFFNSSPVILCFPTHPMTSFHPSTRALKTGPEGSRIRSHE 62
           MAMASILKPPPF PFHSLNP+FFNSSP+ILCFPTHP+ SFHPSTRALKTGPEG R+RSH+
Sbjct: 1   MAMASILKPPPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIRLRSHQ 60

Query: 63  EYSSDLLRKPFVPPAKDLAGISEDDESSEESGNDDQEEEDWVDWEDKILEDTVPLVGFVR 122
           EYSSDLLRKP  P AKDLAG SEDD+SSEESGN+D+EE +WVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVR 120

Query: 123 MLLHSGKYGSGDRLSPEHEKTILDRLLPYHPECEKKIGCGINYITIGYHPDFESSRCLFI 182
           M+LH+GKY +GDRL PEHEKTIL+RLLPYHPE EKKIGCG++YIT+GYHPDFESSRCLFI
Sbjct: 121 MVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 183 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNFRR 228
           VRKDGE+VDFSYWKCIKGLIRKNYPL+AESFILRHFRRRRRN RR
Sbjct: 181 VRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRNSRR 225

[Close]

BLAST of Spg016673 vs. ExPASy TrEMBL
Match: A0A6J1IM57 (protein DCL homolog, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476513 PE=4 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 6.1e-110
Identity = 196/227 (86.34%), Postives = 211/227 (92.95%), Query Frame = 0


Query: 1   MVMAMASILKPPPFVPFHSLNPSFFNSSPVILCFPTHPMTSFHPSTRALKTGPEGSRIRS 60
           MV+AMASILKPPP VP HSLNP+  N+SPVI+CF THPMTSFHPS RALKTGPEGSRIRS
Sbjct: 1   MVLAMASILKPPPLVPLHSLNPNLLNASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRS 60

Query: 61  HEEYSSDLLRKPFVPPAKDLAGISEDDESSEESGNDDQEEEDWVDWEDKILEDTVPLVGF 120
           HEEYSSDLLRKP VPPA DLA  SED  SSEESGN D+EEE+WVDWED+ILE+TVPLVGF
Sbjct: 61  HEEYSSDLLRKPVVPPAMDLATPSEDYNSSEESGNGDEEEEEWVDWEDRILEETVPLVGF 120

Query: 121 VRMLLHSGKYGSGDRLSPEHEKTILDRLLPYHPECEKKIGCGINYITIGYHPDFESSRCL 180
           VRM+LHSGKYGSGDRLSPEHEKTILDRLL YHPECEKKIGCGI+YITIGYHP+FE SRCL
Sbjct: 121 VRMVLHSGKYGSGDRLSPEHEKTILDRLLLYHPECEKKIGCGIDYITIGYHPNFERSRCL 180

Query: 181 FIVRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNFRR 228
           FIVRKDGELVDFSYWKC++GLIRKNYPLHAESFILRHF+RRRR+F R
Sbjct: 181 FIVRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQRRRRHFFR 227

[Close]

BLAST of Spg016673 vs. TAIR 10
Match: AT1G45230.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 270.0 bits (689), Expect = 1.7e-72
Identity = 128/200 (64.00%), Postives = 158/200 (79.00%), Query Frame = 0


Query: 25  FNSSPVILCFPTHPMTSFHPSTRALKTGPEGSRIRSHEEYSSDLLRKPFVPPAKDLAGIS 84
           F+SSP+ L FP    TS  P  RAL+T  +G++I + E Y S+LLR+P +          
Sbjct: 26  FSSSPLCLYFPRGDSTSLRPRVRALRTESDGAKIGNSESYGSELLRRPRI-------ASE 85

Query: 85  EDDESSEESGNDDQEEEDWVDWEDKILEDTVPLVGFVRMLLHSGKYGSGDRLSPEHEKTI 144
           E  E  EE   ++ E +++VDWEDKILE TVPLVGFVRM+LHSGKY + DRLSPEHE+TI
Sbjct: 86  ESSEEEEEEEEENSEGDEFVDWEDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTI 145

Query: 145 LDRLLPYHPECEKKIGCGINYITIGYHPDFESSRCLFIVRKDGELVDFSYWKCIKGLIRK 204
           ++ LLPYHPECEKKIGCGI+YI +G+HPDFESSRC+FIVRKDGE+VDFSYWKCIKGLI+K
Sbjct: 146 IEMLLPYHPECEKKIGCGIDYIMVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKK 205

Query: 205 NYPLHAESFILRHFRRRRRN 225
            YPL+A+SFILRHFR+RR+N
Sbjct: 206 KYPLYADSFILRHFRKRRQN 218

[Close]

BLAST of Spg016673 vs. TAIR 10
Match: AT1G45230.2 (Protein of unknown function (DUF3223) )

HSP 1 Score: 266.9 bits (681), Expect = 1.5e-71
Identity = 127/200 (63.50%), Postives = 157/200 (78.50%), Query Frame = 0


Query: 25  FNSSPVILCFPTHPMTSFHPSTRALKTGPEGSRIRSHEEYSSDLLRKPFVPPAKDLAGIS 84
           F+SSP+ L FP    TS  P  RAL+T  +G++I + E Y S+LLR+P +          
Sbjct: 26  FSSSPLCLYFPRGDSTSLRPRVRALRTESDGAKIGNSESYGSELLRRPRI-------ASE 85

Query: 85  EDDESSEESGNDDQEEEDWVDWEDKILEDTVPLVGFVRMLLHSGKYGSGDRLSPEHEKTI 144
           E  E  EE   ++ E +++VDWEDKILE TVPLVGFVRM+LHSGKY + DRLSPEHE+TI
Sbjct: 86  ESSEEEEEEEEENSEGDEFVDWEDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTI 145

Query: 145 LDRLLPYHPECEKKIGCGINYITIGYHPDFESSRCLFIVRKDGELVDFSYWKCIKGLIRK 204
           ++ LLPYHPECEKKIGCGI+YI + +HPDFESSRC+FIVRKDGE+VDFSYWKCIKGLI+K
Sbjct: 146 IEMLLPYHPECEKKIGCGIDYIMVWHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKK 205

Query: 205 NYPLHAESFILRHFRRRRRN 225
            YPL+A+SFILRHFR+RR+N
Sbjct: 206 KYPLYADSFILRHFRKRRQN 218

[Close]

BLAST of Spg016673 vs. TAIR 10
Match: AT3G46630.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 134.0 bits (336), Expect = 1.5e-31
Identity = 58/125 (46.40%), Postives = 82/125 (65.60%), Query Frame = 0


Query: 96  DDQEEEDWVDWEDKILEDTVPLVGFVRMLLHSGKYGSGDRLSPEHEKTILDRLLPYHPEC 155
           +D +   W + E +IL D  P+    + +LHS +Y  G+RL  E EK ++++LLPYHP  
Sbjct: 80  EDPDYRKWKNLEAEILRDIEPISLLAKEILHSDRYLDGERLDFEDEKIVMEKLLPYHPYS 139

Query: 156 EKKIGCGINYITIGYHPDFESSRCLFIVRKDGELVDFSYWKCIKGLIRKNYPLHAESFIL 215
           + KIGCG+++I +  HP F  SRCLF+VR DG  +DFSY KC++  +R  YP HAE FI 
Sbjct: 140 KDKIGCGLDFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYVRDKYPSHAERFIR 199

Query: 216 RHFRR 221
            HF+R
Sbjct: 200 EHFKR 204

[Close]

BLAST of Spg016673 vs. TAIR 10
Match: AT2G40030.1 (nuclear RNA polymerase D1B )

HSP 1 Score: 97.8 bits (242), Expect = 1.2e-20
Identity = 45/130 (34.62%), Postives = 75/130 (57.69%), Query Frame = 0


Query: 98   QEEEDWVDWEDKILEDTVPLVGFVRMLLHSGKYGSGDRLSPEHEKTILDRLLPYHPECEK 157
            Q  + +   E ++L D  P++  +R ++H   Y  GD +S + +  +L+++L +HP+ E 
Sbjct: 1727 QRLDSFTSEEQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKET 1786

Query: 158  KIGCGINYITIGYHPDFESSRCLFIVRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRH 217
            K+G G+++IT+  H  F  SRC F+V  DG   DFSY K +   + K YP  AE FI ++
Sbjct: 1787 KLGSGVDFITVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKY 1846

Query: 218  FRRRRRNFRR 228
            F + R +  R
Sbjct: 1847 FTKPRPSGNR 1856

[Close]

BLAST of Spg016673 vs. TAIR 10
Match: AT5G62440.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 52.4 bits (124), Expect = 5.6e-07
Identity = 25/59 (42.37%), Postives = 36/59 (61.02%), Query Frame = 0


Query: 144 ILDRLLPYHPECEKKIGCGINYITIGYHPDFESSRCLFIVRKDGELVDFSYWKCIKGLI 203
           +LD +   H E EKKIG GI    +  HP ++ SRC F+VR+D    DFS+ KC+  ++
Sbjct: 105 LLDLIKKGHSEPEKKIGGGIKTFQVRTHPMWK-SRCFFLVREDDTADDFSFRKCVDQIL 162

The following BLAST results are available for this feature:

BLAST of Spg016673 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897137.1	5.1e-119	92.95	protein DCL homolog, chloroplastic [Benincasa hispida]	[more]
XP_022133006.1	2.4e-116	91.96	protein DCL homolog, chloroplastic [Momordica charantia]	[more]
XP_008452556.1	1.6e-112	88.44	PREDICTED: protein DCL, chloroplastic [Cucumis melo]	[more]
KAA0064386.1	1.6e-112	88.44	protein DCL [Cucumis melo var. makuwa] >TYK20201.1 protein DCL [Cucumis melo var...	[more]
XP_004141259.1	1.4e-111	86.67	protein DCL homolog, chloroplastic [Cucumis sativus] >KGN55194.1 hypothetical pr...	[more]

BLAST of Spg016673 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C642	2.4e-71	64.00	Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 S...	[more]
Q42463	2.5e-68	69.44	Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1	[more]
Q5D869	1.6e-19	34.62	DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPE1...	[more]
Q8L557	7.9e-06	42.37	Protein EMBRYO DEFECTIVE 514 OS=Arabidopsis thaliana OX=3702 GN=EMB514 PE=1 SV=1	[more]
P85936	7.4e-04	81.82	Protein DCL, chloroplastic (Fragment) OS=Pseudotsuga menziesii OX=3357 PE=1 SV=1	[more]

BLAST of Spg016673 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BTU2	1.2e-116	91.96	protein DCL homolog, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11100568...	[more]
A0A5A7VDC9	7.7e-113	88.44	Protein DCL OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003370 P...	[more]
A0A1S3BVA4	7.7e-113	88.44	protein DCL, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493543 PE=4 SV=1	[more]
A0A0A0L2D0	6.5e-112	86.67	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639820 PE=4 SV=1	[more]
A0A6J1IM57	6.1e-110	86.34	protein DCL homolog, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476...	[more]

BLAST of Spg016673 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G45230.1	1.7e-72	64.00	Protein of unknown function (DUF3223)	[more]
AT1G45230.2	1.5e-71	63.50	Protein of unknown function (DUF3223)	[more]
AT3G46630.1	1.5e-31	46.40	Protein of unknown function (DUF3223)	[more]
AT2G40030.1	1.2e-20	34.62	nuclear RNA polymerase D1B	[more]
AT5G62440.1	5.6e-07	42.37	Protein of unknown function (DUF3223)	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.40		coord: 112..201 e-value: 2.6E-25 score: 90.7
None	No IPR available	PFAM	PF11523	DUF3223	coord: 123..198 e-value: 2.3E-28 score: 98.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 79..102
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 85..102
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 43..65
None	No IPR available	PANTHER	PTHR33415:SF15	PROTEIN DCL HOMOLOG, CHLOROPLASTIC	coord: 19..224
IPR044673	Protein DCL-like	PANTHER	PTHR33415	FAMILY NOT NAMED	coord: 19..224

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg016673.1	Spg016673.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009658	chloroplast organization
biological_process	GO:1901259	chloroplast rRNA processing
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0003729	mRNA binding