Spg016349 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg016349 vs. NCBI nr
Match: XP_038897279.1 (uncharacterized protein LOC120085399 isoform X1 [Benincasa hispida])

HSP 1 Score: 767.3 bits (1980), Expect = 8.3e-218
Identity = 392/455 (86.15%), Postives = 407/455 (89.45%), Query Frame = 0

Query: 57  PLLSLILLVFFGFWRLLMASAESEKMTSETVSERENRGRMENEKRGELDFNETRNGMRKK 116
           PLL LILL+FFGF RLLMAS E EK TSETVSE  NRG+++NEKRG LD NE RNG RK 
Sbjct: 67  PLL-LILLIFFGFRRLLMASMELEKPTSETVSEGGNRGKLDNEKRGGLDLNEARNGKRK- 126

Query: 117 SGKKNGNRTVSFYLPKIGFGCFRVQRDEEGNVDMEVVNGSGERRKPTHLLIMVNGLVGSA 176
             KK GNRTVSFYLPKIGFGCFRVQRDEEGN+DMEVVNGSGERRKPTHLLIMVNGLVGSA
Sbjct: 127 --KKKGNRTVSFYLPKIGFGCFRVQRDEEGNLDMEVVNGSGERRKPTHLLIMVNGLVGSA 186

Query: 177 KDWKYAAEEFLKTYPEDIVVHCSKRNYSTLTLDGVDVMGGRLAEEILSFIKRHPNVQKIS 236
           KDWKYAAEEFLK Y EDI+VHCSKRNYSTLTLDGVDVMGGRLAEEIL  IKRHPNVQKIS
Sbjct: 187 KDWKYAAEEFLKAYLEDIIVHCSKRNYSTLTLDGVDVMGGRLAEEILLVIKRHPNVQKIS 246

Query: 237 FVCHSLGGLIARYAIAKLYEQDVLKEDVQVNGVGEYNKHGFRDQSNEDEFKGKIAGLEAV 296
           FVCHSLGGLIARYAIAKLYE     +DVQVN  GEYNKHGFRD+S EDEF+GKIAGLE +
Sbjct: 247 FVCHSLGGLIARYAIAKLYE----LKDVQVN--GEYNKHGFRDESCEDEFRGKIAGLEPI 306

Query: 297 NFITCATPHLGSRGHKQVPMCCGFYALEKVAVCTSYFFGRTGRHLFLIDKDSGESPLLFH 356
           NFITCATPHLGSRGHKQVPMCCGFYALEKVAVCTSYFFGRTGRHLFLIDKDSG  PLLFH
Sbjct: 307 NFITCATPHLGSRGHKQVPMCCGFYALEKVAVCTSYFFGRTGRHLFLIDKDSGNCPLLFH 366

Query: 357 MAGDREDLKFLSALQSFRRRVTYANVRYDNVVGWSTSSIRRHKELPKLKGLSGDSKYPYI 416
           MAGDREDLKFLSALQSFRRRVTYANVRYDNVVGWSTSSIRR  ELPKLKGLSGD KYPYI
Sbjct: 367 MAGDREDLKFLSALQSFRRRVTYANVRYDNVVGWSTSSIRRRTELPKLKGLSGDGKYPYI 426

Query: 417 VNEETAKISSPQLYVPSEAKANRYKKADLEEEMIKGLSSVGWERVDVDFHRSKQRNNAHL 476
           VN E  K S+ QLYVPSEAKANR+KK DLEEEMIKG+SSVGWERVDVDFH+SKQRN+AHL
Sbjct: 427 VNVEMEKNSNHQLYVPSEAKANRFKKTDLEEEMIKGISSVGWERVDVDFHKSKQRNDAHL 486

Query: 477 TIQAIQSTCFLQVNRYRVNSDGACVIQHMIDNFLL 512
           TI         QVNRYRVNSDGACVIQHMIDNFLL
Sbjct: 487 TI---------QVNRYRVNSDGACVIQHMIDNFLL 502

BLAST of Spg016349 vs. NCBI nr
Match: XP_008466093.1 (PREDICTED: putative lipase ROG1 [Cucumis melo])

HSP 1 Score: 746.1 bits (1925), Expect = 2.0e-211
Identity = 371/434 (85.48%), Postives = 391/434 (90.09%), Query Frame = 0

Query: 78  ESEKMTSETVSERENRGRMENEKRGELDFNETRNGMRKKSGKKNGNRTVSFYLPKIGFGC 137
           E E   S T SE  NRG +ENEK GELD N+TRNG +KK  KKNGNR+VSFYLPKIGFGC
Sbjct: 2   ELENPASRTSSEEGNRGELENEKGGELDLNDTRNGKKKK--KKNGNRSVSFYLPKIGFGC 61

Query: 138 FRVQRDEEGNVDMEVVNGSGERRKPTHLLIMVNGLVGSAKDWKYAAEEFLKTYPEDIVVH 197
           FRVQRDEEGN+DMEVVNGSGERRKP+HLLIMVNGLVGSAKDWKYAA+EFLKTYPEDI+VH
Sbjct: 62  FRVQRDEEGNIDMEVVNGSGERRKPSHLLIMVNGLVGSAKDWKYAAQEFLKTYPEDIIVH 121

Query: 198 CSKRNYSTLTLDGVDVMGGRLAEEILSFIKRHPNVQKISFVCHSLGGLIARYAIAKLYEQ 257
           CSKRNYSTLTLDGVDVMGGRLAEEIL  IKRHPN++KISF+CHSLGGLIARYAIAKLYE 
Sbjct: 122 CSKRNYSTLTLDGVDVMGGRLAEEILLVIKRHPNLRKISFLCHSLGGLIARYAIAKLYE- 181

Query: 258 DVLKEDVQVNGVGEYNKHGFRDQSNEDEFKGKIAGLEAVNFITCATPHLGSRGHKQVPMC 317
             LKEDVQVN  GEYNKH FRD+S EDEF+GKIAGLE +NFITCATPHLGSRGHKQVPMC
Sbjct: 182 --LKEDVQVN--GEYNKHEFRDESYEDEFRGKIAGLEPINFITCATPHLGSRGHKQVPMC 241

Query: 318 CGFYALEKVAVCTSYFFGRTGRHLFLIDKDSGESPLLFHMAGDREDLKFLSALQSFRRRV 377
           CGFY LEKVAVCTSYFFGRTGRHLFLIDKDSG  PLLFHMAGDREDLKFLSALQSFRRRV
Sbjct: 242 CGFYVLEKVAVCTSYFFGRTGRHLFLIDKDSGNCPLLFHMAGDREDLKFLSALQSFRRRV 301

Query: 378 TYANVRYDNVVGWSTSSIRRHKELPKLKGLSGDSKYPYIVNEETAKISSPQLYVPSEAKA 437
           TYANVRYDNVVGWSTSSIR   ELPKLKGLSGDSKYPYIVN E AKI +PQLYVPSEA+ 
Sbjct: 302 TYANVRYDNVVGWSTSSIRHRSELPKLKGLSGDSKYPYIVNVEMAKIQNPQLYVPSEAEV 361

Query: 438 NRYKKADLEEEMIKGLSSVGWERVDVDFHRSKQRNNAHLTIQAIQSTCFLQVNRYRVNSD 497
           NR+KK+DLEEEMIKGLSSVGW+RVDVDFHRSKQRN+AHLTI         QVNRYRVNSD
Sbjct: 362 NRFKKSDLEEEMIKGLSSVGWDRVDVDFHRSKQRNDAHLTI---------QVNRYRVNSD 419

Query: 498 GACVIQHMIDNFLL 512
           GACVIQHMIDNFLL
Sbjct: 422 GACVIQHMIDNFLL 419

BLAST of Spg016349 vs. NCBI nr
Match: XP_004136232.1 (uncharacterized protein LOC101220342 isoform X2 [Cucumis sativus] >KGN60305.1 hypothetical protein Csa_001617 [Cucumis sativus])

HSP 1 Score: 742.7 bits (1916), Expect = 2.2e-210
Identity = 369/434 (85.02%), Postives = 391/434 (90.09%), Query Frame = 0

Query: 78  ESEKMTSETVSERENRGRMENEKRGELDFNETRNGMRKKSGKKNGNRTVSFYLPKIGFGC 137
           E E   S+T+SE  NRG++ENEK GELD NETRNG +KK  KKNGNR+VSFYLPKIGFGC
Sbjct: 2   ELENPASQTLSEEGNRGKLENEKGGELDLNETRNGKKKK--KKNGNRSVSFYLPKIGFGC 61

Query: 138 FRVQRDEEGNVDMEVVNGSGERRKPTHLLIMVNGLVGSAKDWKYAAEEFLKTYPEDIVVH 197
           FRVQRDEEGNVDMEVVNGSGER+KPTHLLIMVNGLVGSAKDWKYAA+EFLKTYPEDI+VH
Sbjct: 62  FRVQRDEEGNVDMEVVNGSGERQKPTHLLIMVNGLVGSAKDWKYAAQEFLKTYPEDIIVH 121

Query: 198 CSKRNYSTLTLDGVDVMGGRLAEEILSFIKRHPNVQKISFVCHSLGGLIARYAIAKLYEQ 257
           CSKRNYSTLTLDGVDVMGGRLAEEIL  IKRHPNV+KISF+CHSLGGLIARYAIAKLYE 
Sbjct: 122 CSKRNYSTLTLDGVDVMGGRLAEEILLVIKRHPNVRKISFLCHSLGGLIARYAIAKLYE- 181

Query: 258 DVLKEDVQVNGVGEYNKHGFRDQSNEDEFKGKIAGLEAVNFITCATPHLGSRGHKQVPMC 317
             LKEDVQVN  GEYNKH FRD+S EDEF+G+IAGLE +NFITCATPHLGSRGH QVPMC
Sbjct: 182 --LKEDVQVN--GEYNKHEFRDESYEDEFRGRIAGLEPINFITCATPHLGSRGHNQVPMC 241

Query: 318 CGFYALEKVAVCTSYFFGRTGRHLFLIDKDSGESPLLFHMAGDREDLKFLSALQSFRRRV 377
           CGFY LEKVAVCTSYFFGRTGRHLFLID DSG  PLLFHMAGDREDLKFLSALQSFRRRV
Sbjct: 242 CGFYVLEKVAVCTSYFFGRTGRHLFLIDNDSGNCPLLFHMAGDREDLKFLSALQSFRRRV 301

Query: 378 TYANVRYDNVVGWSTSSIRRHKELPKLKGLSGDSKYPYIVNEETAKISSPQLYVPSEAKA 437
           TYANVRYDNVVGWSTSSIRR  ELPK KGLSGDSKYPYIVN E AKI +PQLYVPSEA+ 
Sbjct: 302 TYANVRYDNVVGWSTSSIRRRTELPKRKGLSGDSKYPYIVNVEMAKIQNPQLYVPSEAEV 361

Query: 438 NRYKKADLEEEMIKGLSSVGWERVDVDFHRSKQRNNAHLTIQAIQSTCFLQVNRYRVNSD 497
            R+KK++LEEEMIKGLSSVGWERVDVDFHRSKQRN+AHLTI         QVNRYRVNSD
Sbjct: 362 KRFKKSELEEEMIKGLSSVGWERVDVDFHRSKQRNDAHLTI---------QVNRYRVNSD 419

Query: 498 GACVIQHMIDNFLL 512
           GACV+QHMIDNFLL
Sbjct: 422 GACVVQHMIDNFLL 419

BLAST of Spg016349 vs. NCBI nr
Match: XP_031739315.1 (uncharacterized protein LOC101220342 isoform X1 [Cucumis sativus])

HSP 1 Score: 729.6 bits (1882), Expect = 1.9e-206
Identity = 368/452 (81.42%), Postives = 391/452 (86.50%), Query Frame = 0

Query: 78  ESEKMTSETVSERENRGRMENEKRGELDFNETRNGMRKKSGKKNGNRTVSFYLPKIGFGC 137
           E E   S+T+SE  NRG++ENEK GELD NETRNG +KK  KKNGNR+VSFYLPKIGFGC
Sbjct: 2   ELENPASQTLSEEGNRGKLENEKGGELDLNETRNGKKKK--KKNGNRSVSFYLPKIGFGC 61

Query: 138 FRVQRDEEGNVDMEVVNGSGERRKPTHLLIMVNGLVGSAKDWKYAAEEFLKTYPEDIVVH 197
           FRVQRDEEGNVDMEVVNGSGER+KPTHLLIMVNGLVGSAKDWKYAA+EFLKTYPEDI+VH
Sbjct: 62  FRVQRDEEGNVDMEVVNGSGERQKPTHLLIMVNGLVGSAKDWKYAAQEFLKTYPEDIIVH 121

Query: 198 CSKRNYSTLTLDGVDVMGGRLAEEILSFIKRHPNVQKISFVCHSLGGLIARYAIAKLYEQ 257
           CSKRNYSTLTLDGVDVMGGRLAEEIL  IKRHPNV+KISF+CHSLGGLIARYAIAKLYE 
Sbjct: 122 CSKRNYSTLTLDGVDVMGGRLAEEILLVIKRHPNVRKISFLCHSLGGLIARYAIAKLYE- 181

Query: 258 DVLKEDVQVNGVGEYNKHGFRDQSNEDEFKGKIAGLEAVNFITCATPHLGSRGHKQVPMC 317
             LKEDVQVN  GEYNKH FRD+S EDEF+G+IAGLE +NFITCATPHLGSRGH QVPMC
Sbjct: 182 --LKEDVQVN--GEYNKHEFRDESYEDEFRGRIAGLEPINFITCATPHLGSRGHNQVPMC 241

Query: 318 CGFYALEKVAVCTSYFFGRTGRHLFLIDKDSGESPLLFHMAGDREDLKFLSALQSFRRRV 377
           CGFY LEKVAVCTSYFFGRTGRHLFLID DSG  PLLFHMAGDREDLKFLSALQSFRRRV
Sbjct: 242 CGFYVLEKVAVCTSYFFGRTGRHLFLIDNDSGNCPLLFHMAGDREDLKFLSALQSFRRRV 301

Query: 378 TYANVRYDN------------------VVGWSTSSIRRHKELPKLKGLSGDSKYPYIVNE 437
           TYANVRYD+                  VVGWSTSSIRR  ELPK KGLSGDSKYPYIVN 
Sbjct: 302 TYANVRYDSILLLFLYLIIFLQELFKYVVGWSTSSIRRRTELPKRKGLSGDSKYPYIVNV 361

Query: 438 ETAKISSPQLYVPSEAKANRYKKADLEEEMIKGLSSVGWERVDVDFHRSKQRNNAHLTIQ 497
           E AKI +PQLYVPSEA+  R+KK++LEEEMIKGLSSVGWERVDVDFHRSKQRN+AHLTI 
Sbjct: 362 EMAKIQNPQLYVPSEAEVKRFKKSELEEEMIKGLSSVGWERVDVDFHRSKQRNDAHLTI- 421

Query: 498 AIQSTCFLQVNRYRVNSDGACVIQHMIDNFLL 512
                   QVNRYRVNSDGACV+QHMIDNFLL
Sbjct: 422 --------QVNRYRVNSDGACVVQHMIDNFLL 437

BLAST of Spg016349 vs. NCBI nr
Match: XP_023525017.1 (putative lipase YDL109C [Cucurbita pepo subsp. pepo])

HSP 1 Score: 715.7 bits (1846), Expect = 2.9e-202
Identity = 361/438 (82.42%), Postives = 383/438 (87.44%), Query Frame = 0

Query: 74  MASAESEKMTSETVSERENRGRMENEKRGELDFNETRNGMRKKSGKKNGNRTVSFYLPKI 133
           MAS E  K + E +S+ E R R+EN KR E D N+TRNGM     KKNGNRTVS  LP +
Sbjct: 1   MASVELGKPSYEAMSQGETRARLENGKREETDLNDTRNGM-----KKNGNRTVSSSLPML 60

Query: 134 GFGCFRVQRDEEGNVDMEVVNGSGERRKPTHLLIMVNGLVGSAKDWKYAAEEFLKTYPED 193
           G GCFRVQRDEEGN+D++VVNGSGER +PTHLLIMVNGL GSAKDWKYAAE FLK YPED
Sbjct: 61  GCGCFRVQRDEEGNLDIDVVNGSGERPQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPED 120

Query: 194 IVVHCSKRNYSTLTLDGVDVMGGRLAEEILSFIKRHPNVQKISFVCHSLGGLIARYAIAK 253
           IVVHCSKRNYSTLTLDGVDVMG RLAEEILS I+RH +VQKISFVCHSLGGLIARYAIAK
Sbjct: 121 IVVHCSKRNYSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAK 180

Query: 254 LYEQDVLKEDVQVNGVGEYNKHGFRDQSNEDEFKGKIAGLEAVNFITCATPHLGSRGHKQ 313
           LYEQD LKEDVQVN  GEY+KHG RDQS E+EFKGKIAGLE +NFITCATPHLGSRGHKQ
Sbjct: 181 LYEQDALKEDVQVN--GEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQ 240

Query: 314 VPMCCGFYALEKVAVCTSYFFGRTGRHLFLIDKDSGESPLLFHMAGDREDLKFLSALQSF 373
           VPMCCGFYALEKVAV TSYFFGRTGR LFLIDKDSG+ PLLFHMAGDREDLKFLSALQSF
Sbjct: 241 VPMCCGFYALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSF 300

Query: 374 RRRVTYANVRYDNVVGWSTSSIRRHKELPKLKGLSGDSKYPYIVNEETAKISSPQLYVPS 433
           RRRVTYAN RYDNVVGW TSSIRRHKELPKLKGLSGDS+YPYIVN E AKISSP+LYVPS
Sbjct: 301 RRRVTYANARYDNVVGWCTSSIRRHKELPKLKGLSGDSEYPYIVNVEMAKISSPELYVPS 360

Query: 434 EAKANRYKKADLEEEMIKGLSSVGWERVDVDFHRSKQRNNAHLTIQAIQSTCFLQVNRYR 493
           EA+AN YKKA LEEEMIKGLSSV WERVDVDFH+SKQRNNAHLTI         QVNRY+
Sbjct: 361 EAEANGYKKAVLEEEMIKGLSSVSWERVDVDFHKSKQRNNAHLTI---------QVNRYK 420

Query: 494 VNSDGACVIQHMIDNFLL 512
            NSDGACVIQHMIDNF+L
Sbjct: 421 DNSDGACVIQHMIDNFIL 422

BLAST of Spg016349 vs. ExPASy Swiss-Prot
Match: P53118 (Putative lipase ROG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ROG1 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 2.5e-15
Identity = 77/273 (28.21%), Postives = 123/273 (45.05%), Query Frame = 0

Query: 160 RKPTHLLIMVNGLVGS-AKDWKYAAEEFL---KTYPEDIVVHCSKRNYSTLTLDGVDVMG 219
           +K  HL+++ +GL  + + D  Y  E+     K YP + +V    R     T  GV  +G
Sbjct: 185 QKKKHLVVLTHGLHSNVSTDLVYIMEQIYKAQKNYPHEQIVVKGYRGNVCQTEKGVKYLG 244

Query: 220 GRLAEEILSFIKRHPNVQKISFVCHSLGGLIARYAIAKLYEQDVLKEDVQVNGVGEYNKH 279
            RLAE I+  +    +++KISFV HSLGGLI  +AIA +YE                   
Sbjct: 245 TRLAEYIIQDL-YDESIRKISFVGHSLGGLIQAFAIAYIYE------------------- 304

Query: 280 GFRDQSNEDEFKGKIAGLEAVNFITCATPHLGSRGHKQVPMCCGFYALEKVAVCTSYFFG 339
                     +      +  +NFIT A+P LG        +     A  KV +      G
Sbjct: 305 ---------VYPWFFKKVNPINFITLASPLLG--------IVTDNPAYIKVLLSFG-VIG 364

Query: 340 RTGRHLFLIDKDSGESPLLFHMAGDREDLKFLSALQSFRRRVTYANVRYDNVVGWSTSS- 399
           +TG+ L L +      PLL+ ++G    L  +  L+ F+RR  YAN   D +V   T+S 
Sbjct: 365 KTGQDLGLENDVEVGKPLLYLLSG----LPLIEILRRFKRRTVYANAINDGIVPLYTASL 415

Query: 400 --IRRHKELPKLKGLSGDSKYPYIVNEETAKIS 426
             +  +  L +L+ L  +SK   ++N+ +  ++
Sbjct: 425 LFLDYNDILEQLQKLKENSKKSPLINDASTPVN 415

BLAST of Spg016349 vs. ExPASy Swiss-Prot
Match: O14162 (Putative lipase C4A8.10 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4A8.10 PE=3 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 8.3e-11
Identity = 67/261 (25.67%), Postives = 104/261 (39.85%), Query Frame = 0

Query: 161 KPTHLLIMVNGLVGS-AKDWKYAAEEFL---KTYPEDIVVHCSKRNYSTLTLDGVDVMGG 220
           K +HL+++ +G+  +   D +Y  E+ +   K+  E +VV     NY   T  GV  +G 
Sbjct: 303 KNSHLVVLTHGMHSNVGADMEYLKEKLIESSKSVKELVVVRGFTGNYCQ-TEKGVRWLGK 362

Query: 221 RLAEEILSFI----KRHPNVQKISFVCHSLGGLIARYAIAKLYEQDVLKEDVQVNGVGEY 280
           RL E +L          P    IS V HSLGGL+  YA+  ++ +               
Sbjct: 363 RLGEWLLDITGWGSASFPRYSHISVVAHSLGGLVQTYAVGYVHAK--------------- 422

Query: 281 NKHGFRDQSNEDEFKGKIAGLEAVNFITCATPHLGSRGHKQVPMCCGFYALEKVAVCTSY 340
             HG   Q+           +  V F+T ATP LG  G               +    SY
Sbjct: 423 -THGAFFQA-----------IHPVFFVTLATPWLGVAGEHP----------SYIGKALSY 482

Query: 341 -FFGRTGRHLFLIDKDSGESPLLFHMAGDREDLKFLSALQSFRRRVTYANVRYDNVVGWS 400
              G+TG+ L L   +       F +        F  A+  F +R+ +AN   D +V + 
Sbjct: 483 GIIGKTGQDLSLTPLNHSIESRPFLVLMSDPSTPFFQAVSFFEKRILFANTTNDYIVPFG 524

Query: 401 TSSIRRHKELPKLKGLSGDSK 413
           TS++     L K++   G  K
Sbjct: 543 TSAM-EVSSLGKVEEAEGSDK 524

BLAST of Spg016349 vs. ExPASy Swiss-Prot
Match: Q12103 (Putative lipase YDL109C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YDL109C PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 2.4e-10
Identity = 65/260 (25.00%), Postives = 109/260 (41.92%), Query Frame = 0

Query: 163 THLLIMVNGLVGS-AKDWKYAAEEFLKTY---PEDIVVHCSKRNYSTLTLDGVDVMGGRL 222
           +HL+I+ +G   + + D +Y  EE  K     P + +V       +  T  G+  +G  L
Sbjct: 193 SHLVILTHGFQSNVSADMEYLMEEIYKAQMNNPNERLVIKGYMKNACETEKGIKFLGVGL 252

Query: 223 AEEILSFIKRHPNVQKISFVCHSLGGLIARYAIAKLYEQDVLKEDVQVNGVGEYNKHGFR 282
           A  I+  +    +V KISF+ HSLGGL   +AI  +                        
Sbjct: 253 ANYIIDEL-YDDSVGKISFIGHSLGGLTQTFAICYI------------------------ 312

Query: 283 DQSNEDEFKGKIAGLEAVNFITCATPHLG----SRGHKQVPMCCGFYALEKVAVCTSYFF 342
               + ++      +E +NFI+ A+P LG    +  + ++ +  G               
Sbjct: 313 ----KTKYPYFFKKVEPINFISLASPLLGIATSTPNYVKMSLSMG-------------II 372

Query: 343 GRTGRHLFLIDKDSGESPLLFHMAGDREDLKFLSALQSFRRRVTYANVRYDNVVGWSTSS 402
           G TG+ L L D + G+ PLL+ ++ +      +S L  F+RR  YAN   D +V   +SS
Sbjct: 373 GTTGQELGLKDGNYGDKPLLYLLSEE----SLISVLARFKRRTLYANAVNDGIVPLYSSS 406

Query: 403 IRRHKELPKLKGLSGDSKYP 415
           +        L+ L G +  P
Sbjct: 433 LLFLDYSQLLQKLGGQTTAP 406

BLAST of Spg016349 vs. ExPASy Swiss-Prot
Match: Q9P2D6 (Protein FAM135A OS=Homo sapiens OX=9606 GN=FAM135A PE=1 SV=2)

HSP 1 Score: 49.3 bits (116), Expect = 1.5e-04
Identity = 35/97 (36.08%), Postives = 52/97 (53.61%), Query Frame = 0

Query: 164  HLLIMVNGLVGSAKDWKYAAEEFLKTYPE------DIVVHCSKRNYSTLTLDGVDVMGGR 223
            HL++ V+GL G++ D +      +KTY E       I    S+RN +  T    D M  R
Sbjct: 1249 HLIVCVHGLDGNSADLR-----LVKTYIELGLPGGRIDFLMSERNQND-TFADFDSMTDR 1308

Query: 224  LAEEILSFIKRHP-NVQKISFVCHSLGGLIARYAIAK 254
            L +EI+ +I+ +   V KISF+ HSLG LI R  + +
Sbjct: 1309 LLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTR 1339

BLAST of Spg016349 vs. ExPASy Swiss-Prot
Match: Q6NS59 (Protein FAM135A OS=Mus musculus OX=10090 GN=Fam135a PE=1 SV=2)

HSP 1 Score: 49.3 bits (116), Expect = 1.5e-04
Identity = 34/97 (35.05%), Postives = 52/97 (53.61%), Query Frame = 0

Query: 164  HLLIMVNGLVGSAKDWKYAAEEFLKTYPE------DIVVHCSKRNYSTLTLDGVDVMGGR 223
            HL++ V+GL G++ D +      +KTY E       +    S+RN +  T    D M  R
Sbjct: 1240 HLIVCVHGLDGNSADLR-----LVKTYIELGLPGGRVDFLMSERNQND-TFADFDCMTDR 1299

Query: 224  LAEEILSFIKRHP-NVQKISFVCHSLGGLIARYAIAK 254
            L +EI+ +I+ +   V KISF+ HSLG LI R  + +
Sbjct: 1300 LLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTR 1330

BLAST of Spg016349 vs. ExPASy TrEMBL
Match: A0A1S3CRU4 (putative lipase ROG1 OS=Cucumis melo OX=3656 GN=LOC103503619 PE=4 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 9.6e-212
Identity = 371/434 (85.48%), Postives = 391/434 (90.09%), Query Frame = 0

Query: 78  ESEKMTSETVSERENRGRMENEKRGELDFNETRNGMRKKSGKKNGNRTVSFYLPKIGFGC 137
           E E   S T SE  NRG +ENEK GELD N+TRNG +KK  KKNGNR+VSFYLPKIGFGC
Sbjct: 2   ELENPASRTSSEEGNRGELENEKGGELDLNDTRNGKKKK--KKNGNRSVSFYLPKIGFGC 61

Query: 138 FRVQRDEEGNVDMEVVNGSGERRKPTHLLIMVNGLVGSAKDWKYAAEEFLKTYPEDIVVH 197
           FRVQRDEEGN+DMEVVNGSGERRKP+HLLIMVNGLVGSAKDWKYAA+EFLKTYPEDI+VH
Sbjct: 62  FRVQRDEEGNIDMEVVNGSGERRKPSHLLIMVNGLVGSAKDWKYAAQEFLKTYPEDIIVH 121

Query: 198 CSKRNYSTLTLDGVDVMGGRLAEEILSFIKRHPNVQKISFVCHSLGGLIARYAIAKLYEQ 257
           CSKRNYSTLTLDGVDVMGGRLAEEIL  IKRHPN++KISF+CHSLGGLIARYAIAKLYE 
Sbjct: 122 CSKRNYSTLTLDGVDVMGGRLAEEILLVIKRHPNLRKISFLCHSLGGLIARYAIAKLYE- 181

Query: 258 DVLKEDVQVNGVGEYNKHGFRDQSNEDEFKGKIAGLEAVNFITCATPHLGSRGHKQVPMC 317
             LKEDVQVN  GEYNKH FRD+S EDEF+GKIAGLE +NFITCATPHLGSRGHKQVPMC
Sbjct: 182 --LKEDVQVN--GEYNKHEFRDESYEDEFRGKIAGLEPINFITCATPHLGSRGHKQVPMC 241

Query: 318 CGFYALEKVAVCTSYFFGRTGRHLFLIDKDSGESPLLFHMAGDREDLKFLSALQSFRRRV 377
           CGFY LEKVAVCTSYFFGRTGRHLFLIDKDSG  PLLFHMAGDREDLKFLSALQSFRRRV
Sbjct: 242 CGFYVLEKVAVCTSYFFGRTGRHLFLIDKDSGNCPLLFHMAGDREDLKFLSALQSFRRRV 301

Query: 378 TYANVRYDNVVGWSTSSIRRHKELPKLKGLSGDSKYPYIVNEETAKISSPQLYVPSEAKA 437
           TYANVRYDNVVGWSTSSIR   ELPKLKGLSGDSKYPYIVN E AKI +PQLYVPSEA+ 
Sbjct: 302 TYANVRYDNVVGWSTSSIRHRSELPKLKGLSGDSKYPYIVNVEMAKIQNPQLYVPSEAEV 361

Query: 438 NRYKKADLEEEMIKGLSSVGWERVDVDFHRSKQRNNAHLTIQAIQSTCFLQVNRYRVNSD 497
           NR+KK+DLEEEMIKGLSSVGW+RVDVDFHRSKQRN+AHLTI         QVNRYRVNSD
Sbjct: 362 NRFKKSDLEEEMIKGLSSVGWDRVDVDFHRSKQRNDAHLTI---------QVNRYRVNSD 419

Query: 498 GACVIQHMIDNFLL 512
           GACVIQHMIDNFLL
Sbjct: 422 GACVIQHMIDNFLL 419

BLAST of Spg016349 vs. ExPASy TrEMBL
Match: A0A0A0LE49 (DUF676 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G894530 PE=4 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 1.1e-210
Identity = 369/434 (85.02%), Postives = 391/434 (90.09%), Query Frame = 0

Query: 78  ESEKMTSETVSERENRGRMENEKRGELDFNETRNGMRKKSGKKNGNRTVSFYLPKIGFGC 137
           E E   S+T+SE  NRG++ENEK GELD NETRNG +KK  KKNGNR+VSFYLPKIGFGC
Sbjct: 2   ELENPASQTLSEEGNRGKLENEKGGELDLNETRNGKKKK--KKNGNRSVSFYLPKIGFGC 61

Query: 138 FRVQRDEEGNVDMEVVNGSGERRKPTHLLIMVNGLVGSAKDWKYAAEEFLKTYPEDIVVH 197
           FRVQRDEEGNVDMEVVNGSGER+KPTHLLIMVNGLVGSAKDWKYAA+EFLKTYPEDI+VH
Sbjct: 62  FRVQRDEEGNVDMEVVNGSGERQKPTHLLIMVNGLVGSAKDWKYAAQEFLKTYPEDIIVH 121

Query: 198 CSKRNYSTLTLDGVDVMGGRLAEEILSFIKRHPNVQKISFVCHSLGGLIARYAIAKLYEQ 257
           CSKRNYSTLTLDGVDVMGGRLAEEIL  IKRHPNV+KISF+CHSLGGLIARYAIAKLYE 
Sbjct: 122 CSKRNYSTLTLDGVDVMGGRLAEEILLVIKRHPNVRKISFLCHSLGGLIARYAIAKLYE- 181

Query: 258 DVLKEDVQVNGVGEYNKHGFRDQSNEDEFKGKIAGLEAVNFITCATPHLGSRGHKQVPMC 317
             LKEDVQVN  GEYNKH FRD+S EDEF+G+IAGLE +NFITCATPHLGSRGH QVPMC
Sbjct: 182 --LKEDVQVN--GEYNKHEFRDESYEDEFRGRIAGLEPINFITCATPHLGSRGHNQVPMC 241

Query: 318 CGFYALEKVAVCTSYFFGRTGRHLFLIDKDSGESPLLFHMAGDREDLKFLSALQSFRRRV 377
           CGFY LEKVAVCTSYFFGRTGRHLFLID DSG  PLLFHMAGDREDLKFLSALQSFRRRV
Sbjct: 242 CGFYVLEKVAVCTSYFFGRTGRHLFLIDNDSGNCPLLFHMAGDREDLKFLSALQSFRRRV 301

Query: 378 TYANVRYDNVVGWSTSSIRRHKELPKLKGLSGDSKYPYIVNEETAKISSPQLYVPSEAKA 437
           TYANVRYDNVVGWSTSSIRR  ELPK KGLSGDSKYPYIVN E AKI +PQLYVPSEA+ 
Sbjct: 302 TYANVRYDNVVGWSTSSIRRRTELPKRKGLSGDSKYPYIVNVEMAKIQNPQLYVPSEAEV 361

Query: 438 NRYKKADLEEEMIKGLSSVGWERVDVDFHRSKQRNNAHLTIQAIQSTCFLQVNRYRVNSD 497
            R+KK++LEEEMIKGLSSVGWERVDVDFHRSKQRN+AHLTI         QVNRYRVNSD
Sbjct: 362 KRFKKSELEEEMIKGLSSVGWERVDVDFHRSKQRNDAHLTI---------QVNRYRVNSD 419

Query: 498 GACVIQHMIDNFLL 512
           GACV+QHMIDNFLL
Sbjct: 422 GACVVQHMIDNFLL 419

BLAST of Spg016349 vs. ExPASy TrEMBL
Match: A0A6J1FT75 (putative lipase ROG1 OS=Cucurbita moschata OX=3662 GN=LOC111446646 PE=4 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 1.3e-200
Identity = 361/438 (82.42%), Postives = 380/438 (86.76%), Query Frame = 0

Query: 74  MASAESEKMTSETVSERENRGRMENEKRGELDFNETRNGMRKKSGKKNGNRTVSFYLPKI 133
           MAS E  K + E VS+ ENRGR ENEKR      E RNGM     KKNGNRTVS  LP +
Sbjct: 1   MASVELGKPSYEAVSQGENRGRFENEKR-----EEARNGM-----KKNGNRTVSSSLPML 60

Query: 134 GFGCFRVQRDEEGNVDMEVVNGSGERRKPTHLLIMVNGLVGSAKDWKYAAEEFLKTYPED 193
           G GCFRVQRDEEGN+D++VVNGSGER +PTHLLIMVNGL GSAKDWKYAAE FLK YPED
Sbjct: 61  GCGCFRVQRDEEGNLDIDVVNGSGERPQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPED 120

Query: 194 IVVHCSKRNYSTLTLDGVDVMGGRLAEEILSFIKRHPNVQKISFVCHSLGGLIARYAIAK 253
           IVVHCSKRNYSTLTLDGVDVMG RLAEEILS I+ H +VQKISFVCHSLGGLIARYAIAK
Sbjct: 121 IVVHCSKRNYSTLTLDGVDVMGERLAEEILSVIRTHSSVQKISFVCHSLGGLIARYAIAK 180

Query: 254 LYEQDVLKEDVQVNGVGEYNKHGFRDQSNEDEFKGKIAGLEAVNFITCATPHLGSRGHKQ 313
           LYEQD L+EDVQVN  GEY+KHG RDQS E+EFKGKIAGLE +NFITCATPHLGSRGHKQ
Sbjct: 181 LYEQDALREDVQVN--GEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQ 240

Query: 314 VPMCCGFYALEKVAVCTSYFFGRTGRHLFLIDKDSGESPLLFHMAGDREDLKFLSALQSF 373
           VPMCCGFYALEKVAV TSYFFGRTGR LFLIDKDSG+ PLLFHMAGDREDLKFLSALQSF
Sbjct: 241 VPMCCGFYALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSF 300

Query: 374 RRRVTYANVRYDNVVGWSTSSIRRHKELPKLKGLSGDSKYPYIVNEETAKISSPQLYVPS 433
           RRRVTYAN RYDNVVGW TSSIRRHKELPKLKGLSGDSKYPYIVN E AKISSP+LYVPS
Sbjct: 301 RRRVTYANARYDNVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPS 360

Query: 434 EAKANRYKKADLEEEMIKGLSSVGWERVDVDFHRSKQRNNAHLTIQAIQSTCFLQVNRYR 493
           EA+AN YKKA LEEEMIKGLSSV WERVDVDFH+SKQRNNAHLTI         QVNRY+
Sbjct: 361 EAEANGYKKAVLEEEMIKGLSSVSWERVDVDFHKSKQRNNAHLTI---------QVNRYK 417

Query: 494 VNSDGACVIQHMIDNFLL 512
            NSDGACVIQHMIDNF+L
Sbjct: 421 DNSDGACVIQHMIDNFIL 417

BLAST of Spg016349 vs. ExPASy TrEMBL
Match: A0A6J1IV98 (putative lipase ROG1 OS=Cucurbita maxima OX=3661 GN=LOC111480282 PE=4 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 3.2e-199
Identity = 357/438 (81.51%), Postives = 380/438 (86.76%), Query Frame = 0

Query: 74  MASAESEKMTSETVSERENRGRMENEKRGELDFNETRNGMRKKSGKKNGNRTVSFYLPKI 133
           MAS E  K + E +S+ E RGR+ENEKR      E RN M     KKNGNRTVSF LP +
Sbjct: 1   MASVELGKPSYEAMSQGETRGRLENEKR-----EEARNVM-----KKNGNRTVSFSLPML 60

Query: 134 GFGCFRVQRDEEGNVDMEVVNGSGERRKPTHLLIMVNGLVGSAKDWKYAAEEFLKTYPED 193
           G GCFRVQRDEEGN+DM+VVNGSG+RR+PTHLLIMVNGL GSAKDWKYAAE FLK YPED
Sbjct: 61  GCGCFRVQRDEEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPED 120

Query: 194 IVVHCSKRNYSTLTLDGVDVMGGRLAEEILSFIKRHPNVQKISFVCHSLGGLIARYAIAK 253
           +VVHCSKRNYSTLTLDGVDVMG RLAEEILS I+RH +VQKISFVCHSLGGLIARYAIAK
Sbjct: 121 MVVHCSKRNYSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAK 180

Query: 254 LYEQDVLKEDVQVNGVGEYNKHGFRDQSNEDEFKGKIAGLEAVNFITCATPHLGSRGHKQ 313
           LYEQD L+EDVQVN  GEY+KHG RDQS E+EFKGKIAGLE +NFITCATPHLGSRGHKQ
Sbjct: 181 LYEQDALREDVQVN--GEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQ 240

Query: 314 VPMCCGFYALEKVAVCTSYFFGRTGRHLFLIDKDSGESPLLFHMAGDREDLKFLSALQSF 373
           VPMCCGFYALEKVAV TSYFFGRTGR LFLIDKDSG+ PLLFHMAGDREDLKFLSALQSF
Sbjct: 241 VPMCCGFYALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSF 300

Query: 374 RRRVTYANVRYDNVVGWSTSSIRRHKELPKLKGLSGDSKYPYIVNEETAKISSPQLYVPS 433
           RRRVTYAN RYDNVVGW TSSIRRHKELPKLKGLSGDSKYPYIVN E AKISSP+LYVPS
Sbjct: 301 RRRVTYANARYDNVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPS 360

Query: 434 EAKANRYKKADLEEEMIKGLSSVGWERVDVDFHRSKQRNNAHLTIQAIQSTCFLQVNRYR 493
           EA+AN YKKA LEEEMIKGL S+ WERVDVDFH+SKQRNNAHLTI         QVNRY+
Sbjct: 361 EAEANGYKKAVLEEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTI---------QVNRYK 417

Query: 494 VNSDGACVIQHMIDNFLL 512
            NSDGACVIQHMID F+L
Sbjct: 421 DNSDGACVIQHMIDKFIL 417

BLAST of Spg016349 vs. ExPASy TrEMBL
Match: A0A5D3E533 (Putative lipase ROG1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005190 PE=4 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 1.9e-191
Identity = 338/402 (84.08%), Postives = 358/402 (89.05%), Query Frame = 0

Query: 78  ESEKMTSETVSERENRGRMENEKRGELDFNETRNGMRKKSGKKNGNRTVSFYLPKIGFGC 137
           E E   S T SE  NRG +ENEK GELD N+TRNG +KK  KKNGNR+VSFYLPKIGFGC
Sbjct: 2   ELENPASRTSSEEGNRGELENEKGGELDLNDTRNGKKKK--KKNGNRSVSFYLPKIGFGC 61

Query: 138 FRVQRDEEGNVDMEVVNGSGERRKPTHLLIMVNGLVGSAKDWKYAAEEFLKTYPEDIVVH 197
           FRVQRDEEGN+DMEVVNGSGERRKP+HLLIMVNGLVGSAKDWKYAA+EFLKTYPEDI+VH
Sbjct: 62  FRVQRDEEGNIDMEVVNGSGERRKPSHLLIMVNGLVGSAKDWKYAAQEFLKTYPEDIIVH 121

Query: 198 CSKRNYSTLTLDGVDVMGGRLAEEILSFIKRHPNVQKISFVCHSLGGLIARYAIAKLYEQ 257
           CSKRNYSTLTLDGVDVMGGRLAEEIL  IKRHPN++KISF+CHSLGGLIARYAIAKLYE 
Sbjct: 122 CSKRNYSTLTLDGVDVMGGRLAEEILLVIKRHPNLRKISFLCHSLGGLIARYAIAKLYE- 181

Query: 258 DVLKEDVQVNGVGEYNKHGFRDQSNEDEFKGKIAGLEAVNFITCATPHLGSRGHKQVPMC 317
             LKEDVQVN  GEYNKH FRD+S EDEF+GKIAGLE +NFITCATPHLGSRGHKQVPMC
Sbjct: 182 --LKEDVQVN--GEYNKHEFRDESYEDEFRGKIAGLEPINFITCATPHLGSRGHKQVPMC 241

Query: 318 CGFYALEKVAVCTSYFFGRTGRHLFLIDKDSGESPLLFHMAGDREDLKFLSALQSFRRRV 377
           CGFY LEKVAVCTSYFFGRTGRHLFLIDKDSG  PLLFHMAGDREDLKFLSALQSFRRRV
Sbjct: 242 CGFYVLEKVAVCTSYFFGRTGRHLFLIDKDSGNCPLLFHMAGDREDLKFLSALQSFRRRV 301

Query: 378 TYANVRYDNVVGWSTSSIRRHKELPKLKGLSGDSKYPYIVNEETAKISSPQLYVPSEAKA 437
           TYANVRYDNVVGWSTSSIR   ELPKLKGLSGDSKYPYIVN E AKI +PQLYVPSEA+ 
Sbjct: 302 TYANVRYDNVVGWSTSSIRHRSELPKLKGLSGDSKYPYIVNVEMAKIQNPQLYVPSEAEV 361

Query: 438 NRYKKADLE---------EEMIKGLSSVGWERVDVDFHRSKQ 471
           NR+KK+DLE         EEMIKGLSSVGW+RVDVDFHRS +
Sbjct: 362 NRFKKSDLEVSCCIETIVEEMIKGLSSVGWDRVDVDFHRSTE 396

BLAST of Spg016349 vs. TAIR 10
Match: AT1G10040.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 416.8 bits (1070), Expect = 2.6e-116
Identity = 215/397 (54.16%), Postives = 272/397 (68.51%), Query Frame = 0

Query: 113 MRKKSGKKNGNRTVSFYLPKIGFGCFRVQRDEEGNVDMEVVNGSGERRKPTHLLIMVNGL 172
           M+K   +K+ ++       K   GC R + DE GNVD+  V+  GER +PTHL++MVNGL
Sbjct: 31  MKKMRSRKSDDKETKKKKKKYWMGCLRAESDESGNVDL-TVDFPGERTEPTHLVVMVNGL 90

Query: 173 VGSAKDWKYAAEEFLKTYPEDIVVHCSKRNYSTLTLDGVDVMGGRLAEEILSFIKRHPNV 232
           +GSA++W++AA++ LK YP+D++VHCSKRN+ST T DGVDVMG RLAEE+ S IKRHP++
Sbjct: 91  IGSAQNWRFAAKQMLKKYPQDLLVHCSKRNHSTQTFDGVDVMGERLAEEVRSVIKRHPSL 150

Query: 233 QKISFVCHSLGGLIARYAIAKLYEQDVLKEDVQVNGVGEYNKHGFRDQSNEDEFKGKIAG 292
           QKISFV HSLGGLIARYAI +LYEQ+  +E         +N     D+ + +E K +IAG
Sbjct: 151 QKISFVGHSLGGLIARYAIGRLYEQESREE-------LPHNSDDIGDKCSIEEPKARIAG 210

Query: 293 LEAVNFITCATPHLGSRGHKQVPMCCGFYALEKVAVCTSYFFGRTGRHLFLIDKDSGESP 352
           LE V FIT ATPHLGSRGHKQVP+  G Y LE++A   S   G+TG+HLFL D D G+ P
Sbjct: 211 LEPVYFITSATPHLGSRGHKQVPLFSGSYTLERLATRMSGCLGKTGKHLFLADSDGGKPP 270

Query: 353 LLFHMAGDREDLKFLSALQSFRRRVTYANVRYDNVVGWSTSSIRRHKELPKLKGLSGDSK 412
           LL  M  D  DLKF+SALQ F+RR+ YAN  +D++VGWSTSSIRRH ELPKL+    + K
Sbjct: 271 LLLRMVKDSRDLKFISALQCFKRRIAYANTSFDHLVGWSTSSIRRHNELPKLQRGPVNEK 330

Query: 413 YPYIVNEETAKISSPQLYVPSEAKANRYKKADLEEEMIKGLSSVGWERVDVDFHRSKQRN 472
           YP+IVN E    +S      S  KA+R+K  D+EEEMI+ L+ + WERVDV F  + QR 
Sbjct: 331 YPHIVNVEAPDTASNYEEDHSRTKADRFKNLDMEEEMIRELTKLSWERVDVSFRGTLQRF 390

Query: 473 NAHLTIQAIQSTCFLQVNRYRVNSDGACVIQHMIDNF 510
            AH TI         QV    +NS GA VIQHMIDNF
Sbjct: 391 LAHNTI---------QVKTKMINSAGADVIQHMIDNF 410

BLAST of Spg016349 vs. TAIR 10
Match: AT1G10040.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.9e-111
Identity = 202/371 (54.45%), Postives = 260/371 (70.08%), Query Frame = 0

Query: 113 MRKKSGKKNGNRTVSFYLPKIGFGCFRVQRDEEGNVDMEVVNGSGERRKPTHLLIMVNGL 172
           M+K   +K+ ++       K   GC R + DE GNVD+  V+  GER +PTHL++MVNGL
Sbjct: 31  MKKMRSRKSDDKETKKKKKKYWMGCLRAESDESGNVDL-TVDFPGERTEPTHLVVMVNGL 90

Query: 173 VGSAKDWKYAAEEFLKTYPEDIVVHCSKRNYSTLTLDGVDVMGGRLAEEILSFIKRHPNV 232
           +GSA++W++AA++ LK YP+D++VHCSKRN+ST T DGVDVMG RLAEE+ S IKRHP++
Sbjct: 91  IGSAQNWRFAAKQMLKKYPQDLLVHCSKRNHSTQTFDGVDVMGERLAEEVRSVIKRHPSL 150

Query: 233 QKISFVCHSLGGLIARYAIAKLYEQDVLKEDVQVNGVGEYNKHGFRDQSNEDEFKGKIAG 292
           QKISFV HSLGGLIARYAI +LYEQ+  +E         +N     D+ + +E K +IAG
Sbjct: 151 QKISFVGHSLGGLIARYAIGRLYEQESREE-------LPHNSDDIGDKCSIEEPKARIAG 210

Query: 293 LEAVNFITCATPHLGSRGHKQVPMCCGFYALEKVAVCTSYFFGRTGRHLFLIDKDSGESP 352
           LE V FIT ATPHLGSRGHKQVP+  G Y LE++A   S   G+TG+HLFL D D G+ P
Sbjct: 211 LEPVYFITSATPHLGSRGHKQVPLFSGSYTLERLATRMSGCLGKTGKHLFLADSDGGKPP 270

Query: 353 LLFHMAGDREDLKFLSALQSFRRRVTYANVRYDNVVGWSTSSIRRHKELPKLKGLSGDSK 412
           LL  M  D  DLKF+SALQ F+RR+ YAN  +D++VGWSTSSIRRH ELPKL+    + K
Sbjct: 271 LLLRMVKDSRDLKFISALQCFKRRIAYANTSFDHLVGWSTSSIRRHNELPKLQRGPVNEK 330

Query: 413 YPYIVNEETAKISSPQLYVPSEAKANRYKKADLEEEMIKGLSSVGWERVDVDFHRSKQRN 472
           YP+IVN E    +S      S  KA+R+K  D+EEEMI+ L+ + WERVDV F  + QR 
Sbjct: 331 YPHIVNVEAPDTASNYEEDHSRTKADRFKNLDMEEEMIRELTKLSWERVDVSFRGTLQRF 390

Query: 473 NAHLTIQAIQS 484
            AH TIQA ++
Sbjct: 391 LAHNTIQASEN 393

BLAST of Spg016349 vs. TAIR 10
Match: AT4G25770.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 357.8 bits (917), Expect = 1.4e-98
Identity = 193/392 (49.23%), Postives = 247/392 (63.01%), Query Frame = 0

Query: 129 YLPKIGFGCFRVQRDEEGNVDMEVVNGSGE---------RRKPTHLLIMVNGLVGSAKDW 188
           ++ ++G GCF   R +   +  E+ NG  +           KP HL++MVNG+VGSA DW
Sbjct: 50  WMNRLGIGCFAANRSKR-ELTTEIDNGGEDFFDADVMESAEKPDHLVVMVNGIVGSAADW 109

Query: 189 KYAAEEFLKTYPEDIVVHCSKRNYSTLTLDGVDVMGGRLAEEILSFIKRHPNVQKISFVC 248
           KYAAE+F+K +P+ ++VH S+ N +TLT DGVD MG RLA E+L  +K    ++KISFV 
Sbjct: 110 KYAAEQFVKKFPDKVLVHRSESNSATLTFDGVDKMGERLANEVLGVVKHRSGLKKISFVA 169

Query: 249 HSLGGLIARYAIAKLYEQDVLKEDVQVNGVGEYNKHGFRDQSNEDEFK-GKIAGLEAVNF 308
           HSLGGL+ARYAI KLYEQ            GE +       S E   + G+IAGLE +NF
Sbjct: 170 HSLGGLVARYAIGKLYEQP-----------GEVD--SLDSPSKEKSARGGEIAGLEPMNF 229

Query: 309 ITCATPHLGSRGHKQVPMCCGFYALEKVAVCTSYF-FGRTGRHLFLIDKDSGESPLLFHM 368
           IT ATPHLGSRGH+Q P+ CG   LE+ A  T++   GRTG+HLFL+D D G +PLL  M
Sbjct: 230 ITFATPHLGSRGHRQFPILCGLPFLERTASQTAHLAAGRTGKHLFLVDNDDGNAPLLIRM 289

Query: 369 AGDREDLKFLSALQSFRRRVTYANVRYDNVVGWSTSSIRRHKELPKLKGLSGDSKYPYIV 428
           A D +DLKF+SAL +F+RRV YANV +D++VGW TSSIRR  ELPK   L+ D  YP+IV
Sbjct: 290 ATDSDDLKFISALNAFKRRVAYANVNFDSMVGWRTSSIRRPNELPKPNLLATDPNYPHIV 349

Query: 429 NEETAKISSPQLYVPSEAKANRYKKADLEEEMIKGLSSVGWERVDVDFHRSKQRNNAHLT 488
             E   + +      S       +  DLEEEMI GLS + WERVDV FH SKQR  AH T
Sbjct: 350 YVERGNVDNGSCQSTSTVVTE--QDTDLEEEMIHGLSQLSWERVDVSFHNSKQRYVAHST 409

Query: 489 IQAIQSTCFLQVNRYRVNSDGACVIQHMIDNF 510
           I         QV  Y ++SDG  V+ HM+D+F
Sbjct: 410 I---------QVKTYWLHSDGKDVVFHMMDHF 416

BLAST of Spg016349 vs. TAIR 10
Match: AT4G25770.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 340.1 bits (871), Expect = 3.0e-93
Identity = 180/358 (50.28%), Postives = 229/358 (63.97%), Query Frame = 0

Query: 129 YLPKIGFGCFRVQRDEEGNVDMEVVNGSGE---------RRKPTHLLIMVNGLVGSAKDW 188
           ++ ++G GCF   R +   +  E+ NG  +           KP HL++MVNG+VGSA DW
Sbjct: 50  WMNRLGIGCFAANRSKR-ELTTEIDNGGEDFFDADVMESAEKPDHLVVMVNGIVGSAADW 109

Query: 189 KYAAEEFLKTYPEDIVVHCSKRNYSTLTLDGVDVMGGRLAEEILSFIKRHPNVQKISFVC 248
           KYAAE+F+K +P+ ++VH S+ N +TLT DGVD MG RLA E+L  +K    ++KISFV 
Sbjct: 110 KYAAEQFVKKFPDKVLVHRSESNSATLTFDGVDKMGERLANEVLGVVKHRSGLKKISFVA 169

Query: 249 HSLGGLIARYAIAKLYEQDVLKEDVQVNGVGEYNKHGFRDQSNEDEFK-GKIAGLEAVNF 308
           HSLGGL+ARYAI KLYEQ            GE +       S E   + G+IAGLE +NF
Sbjct: 170 HSLGGLVARYAIGKLYEQP-----------GEVD--SLDSPSKEKSARGGEIAGLEPMNF 229

Query: 309 ITCATPHLGSRGHKQVPMCCGFYALEKVAVCTSYF-FGRTGRHLFLIDKDSGESPLLFHM 368
           IT ATPHLGSRGH+Q P+ CG   LE+ A  T++   GRTG+HLFL+D D G +PLL  M
Sbjct: 230 ITFATPHLGSRGHRQFPILCGLPFLERTASQTAHLAAGRTGKHLFLVDNDDGNAPLLIRM 289

Query: 369 AGDREDLKFLSALQSFRRRVTYANVRYDNVVGWSTSSIRRHKELPKLKGLSGDSKYPYIV 428
           A D +DLKF+SAL +F+RRV YANV +D++VGW TSSIRR  ELPK   L+ D  YP+IV
Sbjct: 290 ATDSDDLKFISALNAFKRRVAYANVNFDSMVGWRTSSIRRPNELPKPNLLATDPNYPHIV 349

Query: 429 NEETAKISSPQLYVPSEAKANRYKKADLEEEMIKGLSSVGWERVDVDFHRSKQRNNAH 476
             E   + +      S       +  DLEEEMI GLS + WERVDV FH SKQR  AH
Sbjct: 350 YVERGNVDNGSCQSTSTVVTE--QDTDLEEEMIHGLSQLSWERVDVSFHNSKQRYVAH 391

BLAST of Spg016349 vs. TAIR 10
Match: AT5G51180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 336.3 bits (861), Expect = 4.4e-92
Identity = 188/376 (50.00%), Postives = 241/376 (64.10%), Query Frame = 0

Query: 143 DEEGNVDMEVVNGS--------GERRKPTHLLIMVNGLVGSAKDWKYAAEEFLKTYPEDI 202
           +E G    E V+GS         +     HL++MV+G++GS  DWK+ AE+F+K  P+ +
Sbjct: 6   EENGLCKAESVDGSLDVWSCQNSDSSSADHLVVMVHGILGSTDDWKFGAEQFVKKMPDKV 65

Query: 203 VVHCSKRNYSTLTLDGVDVMGGRLAEEILSFIKRHPNVQKISFVCHSLGGLIARYAIAKL 262
            VHCS++N S LTLDGVDVMG RLA E+L  I+R PN+ KISFV HSLGGL ARYAI KL
Sbjct: 66  FVHCSEKNVSALTLDGVDVMGERLAAEVLDIIQRKPNICKISFVAHSLGGLAARYAIGKL 125

Query: 263 YEQDVLKEDVQVNGVGEYNKHGFRDQSNEDEFKGKIAGLEAVNFITCATPHLGSRGHKQV 322
           Y +   +EDV         K    D S E   KG I GLEA+NFIT ATPHLGS G+KQV
Sbjct: 126 Y-KPANQEDV---------KDSVADSSQETP-KGTICGLEAMNFITVATPHLGSMGNKQV 185

Query: 323 PMCCGFYALEKVA-VCTSYFFGRTGRHLFLIDKDSGESPLLFHMAGDREDLKFLSALQSF 382
           P   GF ++EKVA +   + F RTGRHLFL D++ G+ PLL  M  D +D  F+SAL++F
Sbjct: 186 PFLFGFSSIEKVAGLIIHWIFKRTGRHLFLKDEEEGKPPLLRRMVEDTDDCHFISALRAF 245

Query: 383 RRRVTYANVRYDNVVGWSTSSIRRHKELPKLKGLSGDSKYPYIVNEETAKISSPQLYVPS 442
           +RRV Y+NV +D+VVGW T+SIRR  ELPK +  S + KYP+IV EE  K    +     
Sbjct: 246 KRRVAYSNVGHDHVVGWKTASIRRDSELPKWED-SLNEKYPHIVYEELCKACDMEDIPEG 305

Query: 443 EAKANRYKKADLEEEMIKGLSSVGWERVDVDFHRSKQRNNAHLTIQAIQSTCFLQVNRYR 502
           E  ++     D+EEEMIKGLSSV WE+VDV FH S+QR  AH  I         QV    
Sbjct: 306 ENHSD-----DIEEEMIKGLSSVSWEKVDVSFHSSRQRFAAHSVI---------QVKNED 355

Query: 503 VNSDGACVIQHMIDNF 510
           ++ +GA VI+H+ID+F
Sbjct: 366 MHIEGADVIEHIIDHF 355