Spg016212 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg016212
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	chaperone protein DnaJ
Location	scaffold9: 40663404 .. 40666140 (+)
RNA-Seq Expression	Spg016212
Synteny	Spg016212

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTCTGTGGACACAGTCAAATAATTCTATTTTCTTTCTCTTCCTCGCTTCCTCTCTCTTTCTCTCTCTCATTCATGGTGGATCTTCCTCAGGCTCTTGGCATCTCCTTTCGAAACTTCTGCAAATCCTACAAATCCTTCTTCAAGAAATGGCATTCTAAGGTCAAGAATAATCATTCCAAGAAGGATTCCGATGTTGTTGATCCTAAACACGACGCCTCCTCCGACGCCATTGATAACAATGGACCTTCTAAGGTACGTACGTGATACGTCCATAGTTTTGATGTTTAAGCGTGCTTTTATGAGATCTCGAATTCGAATTGATTGTGTAAATTCGCTTTTCTAATTTGTATTTCGTTTTTGTTCTGTACCGATTACGGATTTGCGTTTGATTTTGGTCAATTGGAATCCGAACCTATGAATTGGAGGATGAAGAAGCCCTAATTTGGAGAAATTTAGGTGTTTAATCGATAATAATCATTGTTTTCAGGATCGTGAAGATGTTAATAGTAAAGGATCGAGACCGACGACGACGAAAGGCGTTCCAACGAGCTTTGGATATGGAGGCCAGAGCATGAGGGAGAATGATCGGACGAGTTACAGGCCTCGAAGCTACGACAGCGGATTCTCTTCTCTGTCCCGAAATGCGAGTCGGAAAGGTCAGAATCTGCGTTCGTCGTCGTCGTCCCAGTTTCGGAGCGTGAGCCGGAGCACGGATTCGGTGCCGTCTCGGGTGAGCAGCGGGCGGAGAAGCATCGATTCGACGTCGTCTTCTCCGTTGCTGTCGAAAAGCAGCAGTAGTCGGAGGAGCACGACGCCGATCATGTTCTCGAATTCCTCCGGCCTGCTGAAGCCGGCGCCGATCGAGAAGCAGCTCGAATGCACTCTGGAGGAGCTCTGCTTCGGATGCATGAAGAAAATCAAGGTCACGAGAGACGTCCTCTCAAGGACAGGGTTCGTCCTCTTCTTCTTAAACCATTTCGCCCTTTCCCTTTCCACACACAGCATTTTCAATACATAAATCTTTCCATTCTGAATATCCAAGAGACAAATCAGAGGAGTTTCTTTAAGATCTTTTTTTTAAAAAAAGAAATTATAAAATTCCTCTCTAAATTGTGTCTGTTTAATATCAAATTTTAAATAGATGTGACAAATTTCTAAGAAAAATATTTGGCTGCATGCTAATGGTTCCTATTCACCACCATTGAATTTTTTAAAGTTAGATTCAATTTCTTATTTTCTACCTCTCTTTGTACCAGAAAAAATACTTACCCAAGTAATATTAAACTTTTGGTATCATACATATATATTTTTTTAAATTTTAGTTCAATAAGCATAAGTAGCAGTAAAGGTAAAAATAACTCAATGGATCATCACATTATAATTTATCCACCCTGTTTTAGTACATCAATATTTGGGGATTCAAACCTCTGACCTCTTGATTGAAGGCATATGCCAATTACCGCTGAGCTAGACTCACTTTGGCATCTAAGAATTAAATTTTTTTAATATTGTCATTGTTTGGTTGATTTTTTGGATACTTATGTGACATTATTCAAGAAGATGTGTCACAAACTATATTATTTAAGATGTGACGTGTTGGTTTTTGGCACAATGAACCCACCTGACATGAAAATACAAGTGAAGCAAACATATTGAAAACCATTTAAACTTTTTAAAACAAACACAAATTATAATTCAAGGTAACAATTTAACCTCCTCCTAAGAAATTCAATTGAGAAAAATAATTCAACCCCAATGACCATTTGGTTTTTGGTTTTTTAAAATTATGCTTGTTTTCTCCTAAATTCCTTACCATAGCTTTCATCCTAGTTAAGGTTGCATTTGAATTCCTAGTCAAATTTTAAATATTTTTTTTTTTTGAAAACAAGGTAGAAGATTGATATTAAAACTAAGAAACTTATAGGTGGGAGTACGTGTGTATAAGTTTAATGGTTATGAAACGGGACATGAGAGATTCCATTTGTGATTGACAGGCAGGCAAGAGAGGAAGAGGAAACGCTGACGATAAAAGTAAAGCCAGGGTGGAAGAAAGGGACGAAGATAACATTCGAAGGAATGGGGAACGAGAGGCCAGGCAGTTACCCAGCCGACATGTCGTTCGTGATAGCCGAGAAACGACACTCGTATTTCAAGAGAGAAGGCGACGACTTGGAGCTGACAGTGGAGATTCCATTGGTGAAAGCTCTGACTGGTTGCACAATTTCAGTGCCTCTGTTGGGAGGAGAAGCGATGAGCTTGGAGATACACGACGTCGTTTGGCCTGGCTACCAGAAGATCATCAAAGGCCAAGGTATGCCTAAACCTAAAGATGATGAAAACAGAGGCAACTTGAAAGTTAAGTTTCTGGTTGAGTTTCCAACCCAATTAACTCACCAACAAAGATCTGATGTTTGTCGAATTTTAGACGCTTCTGACTATTCTAGTTGAGGGATAATTATTATTGGATTTTATTTTCTTTAGGAATTGACTCTCTTTATGTATTTCTCTTTTGTTTTATATTGTCTGTCTCTAATTTATGTTTTGGTTTTTGAGTGGTGAAGGAAAATTGCATTTTTTGTGCATTTGATTGAGAGACAAACCCCGTCATTTCATTTGATTTATTCTTTTAGGTTTAAATACTATTTTAGTCTCTATACTTTTGGCTCTTGGGTTTTGTTCATTTTAGCCTTGTACTTTCAAAATAGTCATTTTGGTCTTGTACTTTTACAAATCGGC

mRNA sequence

ATGGTGGATCTTCCTCAGGCTCTTGGCATCTCCTTTCGAAACTTCTGCAAATCCTACAAATCCTTCTTCAAGAAATGGCATTCTAAGGTCAAGAATAATCATTCCAAGAAGGATTCCGATGTTGTTGATCCTAAACACGACGCCTCCTCCGACGCCATTGATAACAATGGACCTTCTAAGGATCGTGAAGATGTTAATAGTAAAGGATCGAGACCGACGACGACGAAAGGCGTTCCAACGAGCTTTGGATATGGAGGCCAGAGCATGAGGGAGAATGATCGGACGAGTTACAGGCCTCGAAGCTACGACAGCGGATTCTCTTCTCTGTCCCGAAATGCGAGTCGGAAAGGTCAGAATCTGCGTTCGTCGTCGTCGTCCCAGTTTCGGAGCGTGAGCCGGAGCACGGATTCGGTGCCGTCTCGGGTGAGCAGCGGGCGGAGAAGCATCGATTCGACGTCGTCTTCTCCGTTGCTGTCGAAAAGCAGCAGTAGTCGGAGGAGCACGACGCCGATCATGTTCTCGAATTCCTCCGGCCTGCTGAAGCCGGCGCCGATCGAGAAGCAGCTCGAATGCACTCTGGAGGAGCTCTGCTTCGGATGCATGAAGAAAATCAAGGTCACGAGAGACGTCCTCTCAAGGACAGGGCAGGCAAGAGAGGAAGAGGAAACGCTGACGATAAAAGTAAAGCCAGGGTGGAAGAAAGGGACGAAGATAACATTCGAAGGAATGGGGAACGAGAGGCCAGGCAGTTACCCAGCCGACATGTCGTTCGTGATAGCCGAGAAACGACACTCGTATTTCAAGAGAGAAGGCGACGACTTGGAGCTGACAGTGGAGATTCCATTGGTGAAAGCTCTGACTGGTTGCACAATTTCAGTGCCTCTGTTGGGAGGAGAAGCGATGAGCTTGGAGATACACGACGTCGTTTGGCCTGGCTACCAGAAGATCATCAAAGGCCAAGGTATGCCTAAACCTAAAGATGATGAAAACAGAGGCAACTTGAAAGTTAAGTTTCTGGTTGAGTTTCCAACCCAATTAACTCACCAACAAAGATCTGATGTTTGTCGAATTTTAGACGCTTCTGACTATTCTAGTTGA

Coding sequence (CDS)

ATGGTGGATCTTCCTCAGGCTCTTGGCATCTCCTTTCGAAACTTCTGCAAATCCTACAAATCCTTCTTCAAGAAATGGCATTCTAAGGTCAAGAATAATCATTCCAAGAAGGATTCCGATGTTGTTGATCCTAAACACGACGCCTCCTCCGACGCCATTGATAACAATGGACCTTCTAAGGATCGTGAAGATGTTAATAGTAAAGGATCGAGACCGACGACGACGAAAGGCGTTCCAACGAGCTTTGGATATGGAGGCCAGAGCATGAGGGAGAATGATCGGACGAGTTACAGGCCTCGAAGCTACGACAGCGGATTCTCTTCTCTGTCCCGAAATGCGAGTCGGAAAGGTCAGAATCTGCGTTCGTCGTCGTCGTCCCAGTTTCGGAGCGTGAGCCGGAGCACGGATTCGGTGCCGTCTCGGGTGAGCAGCGGGCGGAGAAGCATCGATTCGACGTCGTCTTCTCCGTTGCTGTCGAAAAGCAGCAGTAGTCGGAGGAGCACGACGCCGATCATGTTCTCGAATTCCTCCGGCCTGCTGAAGCCGGCGCCGATCGAGAAGCAGCTCGAATGCACTCTGGAGGAGCTCTGCTTCGGATGCATGAAGAAAATCAAGGTCACGAGAGACGTCCTCTCAAGGACAGGGCAGGCAAGAGAGGAAGAGGAAACGCTGACGATAAAAGTAAAGCCAGGGTGGAAGAAAGGGACGAAGATAACATTCGAAGGAATGGGGAACGAGAGGCCAGGCAGTTACCCAGCCGACATGTCGTTCGTGATAGCCGAGAAACGACACTCGTATTTCAAGAGAGAAGGCGACGACTTGGAGCTGACAGTGGAGATTCCATTGGTGAAAGCTCTGACTGGTTGCACAATTTCAGTGCCTCTGTTGGGAGGAGAAGCGATGAGCTTGGAGATACACGACGTCGTTTGGCCTGGCTACCAGAAGATCATCAAAGGCCAAGGTATGCCTAAACCTAAAGATGATGAAAACAGAGGCAACTTGAAAGTTAAGTTTCTGGTTGAGTTTCCAACCCAATTAACTCACCAACAAAGATCTGATGTTTGTCGAATTTTAGACGCTTCTGACTATTCTAGTTGA

Protein sequence

MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNNHSKKDSDVVDPKHDASSDAIDNNGPSKDREDVNSKGSRPTTTKGVPTSFGYGGQSMRENDRTSYRPRSYDSGFSSLSRNASRKGQNLRSSSSSQFRSVSRSTDSVPSRVSSGRRSIDSTSSSPLLSKSSSSRRSTTPIMFSNSSGLLKPAPIEKQLECTLEELCFGCMKKIKVTRDVLSRTGQAREEEETLTIKVKPGWKKGTKITFEGMGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISVPLLGGEAMSLEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQRSDVCRILDASDYSS

Homology

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BLAST of Spg016212 vs. NCBI nr
Match: XP_038899191.1 (dnaJ homolog subfamily B member 4-like [Benincasa hispida])

HSP 1 Score: 558.9 bits (1439), Expect = 3.2e-155
Identity = 303/365 (83.01%), Postives = 323/365 (88.49%), Query Frame = 0


Query: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNNHSKKDSDVVDPKHDASSDAIDNNGPSK 60
           MVDLPQALGISFRNFCKSYKSFFKKWHSK KN  SKKD+DVVD KHDA+SDAI NNGPSK
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKAKN--SKKDADVVDTKHDATSDAIGNNGPSK 60

Query: 61  DREDVNSKGSRPTTTKGVPTSFGYGGQSMRENDRTSYRPRSYDSGFSSLSRNASRKGQNL 120
           D E+V  K   PTT K V  S  YGGQSMREND TS+RPRSYDSG SSLSRNASR+GQN 
Sbjct: 61  DHEEV-KKTEGPTTMKSV-HSLRYGGQSMRENDATSFRPRSYDSGVSSLSRNASRRGQNP 120

Query: 121 RSSSSSQFRSVS-RSTDSVPSRVSSGRRSIDSTSSSPLLSKSSSSRRSTTPIMFSNSSGL 180
            S+SSS FRSVS RST+SVPSRVSSGRRSIDS SSSPLLSK S SR STTPIMFSNSSGL
Sbjct: 121 SSTSSSLFRSVSRRSTESVPSRVSSGRRSIDSVSSSPLLSK-SGSRMSTTPIMFSNSSGL 180

Query: 181 LKPAPIEKQLECTLEELCFGCMKKIKVTRDVLSRTGQAREEEETLTIKVKPGWKKGTKIT 240
           LKPAPIEKQLECTLEELCFGCMKKIKVTRD++  +G+  EEEETLTIK+KPGW KGTKIT
Sbjct: 181 LKPAPIEKQLECTLEELCFGCMKKIKVTRDLILISGKTMEEEETLTIKIKPGWTKGTKIT 240

Query: 241 FEGMGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISVPLLGGE 300
           FEGMGNERPGSYPADMSFVI EKRH YFKREGDDLELTVEIPL+KAL GC ISVPLLGGE
Sbjct: 241 FEGMGNERPGSYPADMSFVIVEKRHPYFKREGDDLELTVEIPLLKALIGCNISVPLLGGE 300

Query: 301 AMSLEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQRSDVCRILD 360
            MSL+IHDVVWPGY+K+I+GQGMPK KDD+ RGNLKVKFLVEFPT+LT QQR DVCRIL+
Sbjct: 301 TMSLKIHDVVWPGYEKVIQGQGMPKLKDDK-RGNLKVKFLVEFPTELTDQQRYDVCRILE 359

Query: 361 ASDYS 365
            SDYS
Sbjct: 361 DSDYS 359

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BLAST of Spg016212 vs. NCBI nr
Match: XP_008444178.1 (PREDICTED: chaperone protein DnaJ [Cucumis melo])

HSP 1 Score: 535.0 bits (1377), Expect = 4.9e-148
Identity = 297/370 (80.27%), Postives = 321/370 (86.76%), Query Frame = 0


Query: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNNHSKKDSD-VVDPKH-DASSDAIDNNGP 60
           MVDLPQALGISFRNFCKSYKSFFKKWHSKVKN  SKKD+D VVD KH DA+SDAI N+ P
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKN--SKKDTDNVVDTKHDDATSDAIHNHSP 60

Query: 61  SKDREDVNSK-GSRPTTTKGVPTSFGYGGQSMRENDRTSYRPRSYDSGFSSLSRNASRKG 120
           SKD E++  K G RPTTT     SF YGG+S+REND TS+RPRSYDSG+S+LSRNASR+G
Sbjct: 61  SKDHEEIKKKEGPRPTTTTRGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG 120

Query: 121 QNLRSSSSSQFRSVS-RSTDSVPSRVSSGRRSIDSTSSSPLLSKSSSSRRSTTPIMFSNS 180
           QN  S+SSS FRSVS RS +S+PSRVSSGRRSIDS SSSP+LSK S SRRSTTPIMFSNS
Sbjct: 121 QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPVLSK-SGSRRSTTPIMFSNS 180

Query: 181 SGLLKPAPIEKQLECTLEELCFGCMKKIKVTRDVLSRTGQAREEEETLTIKVKPGWKKGT 240
           SG+LK APIEKQLECTLEELCFGCMKKIKVTRD+L   GQA EEEETLTIKVKPGW+KGT
Sbjct: 181 SGVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLINGQAMEEEETLTIKVKPGWRKGT 240

Query: 241 KITFE-GMGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISVPL 300
           KITFE G GNER GSYPAD SFVIAEKRHSYFKREGDDLEL VEIPL+KALTGCTI VPL
Sbjct: 241 KITFEGGTGNERAGSYPADTSFVIAEKRHSYFKREGDDLELMVEIPLLKALTGCTIPVPL 300

Query: 301 LGGEAMSLEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQRSDVC 360
           LGGE MSLEIH+VV PGY+K+I+GQGMPK KD  NRGNL VKF V FPTQLT QQRSDVC
Sbjct: 301 LGGETMSLEIHEVVSPGYEKLIQGQGMPKLKDPNNRGNLIVKFFVHFPTQLTPQQRSDVC 360

Query: 361 RILDASDYSS 366
           RIL+AS Y S
Sbjct: 361 RILEASHYPS 367

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BLAST of Spg016212 vs. NCBI nr
Match: XP_022941087.1 (dnaJ homolog subfamily B member 5-like [Cucurbita moschata])

HSP 1 Score: 516.5 bits (1329), Expect = 1.8e-142
Identity = 281/367 (76.57%), Postives = 314/367 (85.56%), Query Frame = 0


Query: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNNHSKKDSDVVDPKHDASSDAID--NNGP 60
           MVDLPQ LG+SFRNFCKSYKSFFKKWHSKVKN  SKK++DVVD KHD SSDAID  NNGP
Sbjct: 1   MVDLPQVLGVSFRNFCKSYKSFFKKWHSKVKN--SKKNTDVVDSKHDGSSDAIDNNNNGP 60

Query: 61  SKDREDVNSKGSRPTTTKGVPTSFGYGGQSMRENDRTSYRPRSYDSGFSSLSRNASRKGQ 120
           SKDRE++N +G R  T KG   SF Y  +SMREND    R RSYD   SS+ RNASR+G 
Sbjct: 61  SKDREEINKEGPRQMTKKG-GLSFRYASRSMRENDPKISRHRSYD---SSMFRNASRRGL 120

Query: 121 NLRSSSSSQFRSVS-RSTDSVPSRVSSGRRSIDSTSSSPLLSKSSSSRRSTTPIMFSNSS 180
           N + SSSSQFRSVS RST+S+PSR++SG  S+DS SSSP+LSK S SRRSTTPIMFSNSS
Sbjct: 121 NPQPSSSSQFRSVSRRSTESMPSRLNSG-LSVDSASSSPMLSK-SGSRRSTTPIMFSNSS 180

Query: 181 GLLKPAPIEKQLECTLEELCFGCMKKIKVTRDVLSRTGQAREEEETLTIKVKPGWKKGTK 240
           G+LKP PIEKQLECTLEELCFGCMKKIKVTRDV+  TGQA EEEET+TI+VKPGW KGTK
Sbjct: 181 GVLKPPPIEKQLECTLEELCFGCMKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTK 240

Query: 241 ITFEGMGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISVPLLG 300
           ITFEGMGNERPGSYPAD+SFVI EK+H YFKREGDDL+LTVEIPL+KALTGCTIS+PLLG
Sbjct: 241 ITFEGMGNERPGSYPADISFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLG 300

Query: 301 GEAMSLEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQRSDVCRI 360
           G+ MSL  HDV+W G  KIIKGQGMPK K++ NRGNLKVKFL+EFPT+LT QQRSDVCRI
Sbjct: 301 GDVMSLRTHDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRI 359

Query: 361 LDASDYS 365
           L+ S+YS
Sbjct: 361 LEGSNYS 359

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BLAST of Spg016212 vs. NCBI nr
Match: XP_022132204.1 (uncharacterized protein LOC111005118 [Momordica charantia])

HSP 1 Score: 516.5 bits (1329), Expect = 1.8e-142
Identity = 295/366 (80.60%), Postives = 316/366 (86.34%), Query Frame = 0


Query: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNNHSKKDSDVVDPKHDASSDAIDNNGPSK 60
           MVDLPQA+GISFRNFCKSYKSFFKKWHSK+K+  SKKDSD VD K + SSDAID  GPSK
Sbjct: 1   MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKS--SKKDSD-VDQKREGSSDAID--GPSK 60

Query: 61  DREDVNSKG---SRPTTTKGVPTSFGYGGQSMREN-DRTSYRPRSYDSGFSSLSRNASRK 120
           DREDV  +G   +  T TKGVP SF YGG SMREN D  S+RPRSYDSGF SLSRNASRK
Sbjct: 61  DREDVKREGRATATATATKGVP-SFRYGG-SMRENHDPLSFRPRSYDSGF-SLSRNASRK 120

Query: 121 GQNLRSSSSSQFRSVSR--STDSVPSRVSSGRRSIDSTSSSPLLSKSSSSRRSTTPIMFS 180
            QN   SSS  FRSVSR  +  SVPSRVSSGRRS+D  SS PLLSK S SRRSTTPIMFS
Sbjct: 121 SQNTH-SSSPLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSK-SGSRRSTTPIMFS 180

Query: 181 NSSGLLKPAPIEKQLECTLEELCFGCMKKIKVTRDVLSR-TGQAREEEETLTIKVKPGWK 240
           NSSG+LKPAPIEKQLECTLEELCFGC KKIK+TRDVLS  TGQ  EEEETLTI VKPGWK
Sbjct: 181 NSSGVLKPAPIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWK 240

Query: 241 KGTKITFEGMGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISV 300
           KGTKITFEGMGNERPGSYPADMSFVIAEKRH++FK EGDDLE+TVEIPL+KALTGCTISV
Sbjct: 241 KGTKITFEGMGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISV 300

Query: 301 PLLGGEAMSLEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQRSD 360
           PLLGGEAMSLEI DVV PGY++II+GQGMP PKDD+ RG+LKVKFLVEFPTQLT QQRSD
Sbjct: 301 PLLGGEAMSLEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSD 356

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BLAST of Spg016212 vs. NCBI nr
Match: XP_004142703.1 (uncharacterized protein LOC101218142 [Cucumis sativus] >KGN54553.1 hypothetical protein Csa_012239 [Cucumis sativus])

HSP 1 Score: 516.2 bits (1328), Expect = 2.4e-142
Identity = 285/368 (77.45%), Postives = 318/368 (86.41%), Query Frame = 0


Query: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNNHSKKDSDVVDPKH-DASSDAIDNNGPS 60
           MVDLPQALGISFRNFCKSYKSFFKKWHSKVKN+    D+ VVD KH D +SDAI N GPS
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDPTSDAIHNYGPS 60

Query: 61  KDREDVNSK-GSRPTTTKGVPTSFGYGGQSMRENDRTSYRPRSYDSGFSSLSRNASRKGQ 120
           KD ED+  K G RP  TKGV  SF YGG+S+REND TS+RP+SYDSG+S+LSRNASR+GQ
Sbjct: 61  KDHEDIKKKEGPRP--TKGV-HSFRYGGRSLRENDTTSFRPQSYDSGYSTLSRNASRRGQ 120

Query: 121 NLRSSSSSQFRSVS-RSTDSVPSRVSSGRRSIDSTSSSPLLSKSSSSRRSTTPIMFSNSS 180
           N  S+SSS FRS+S RS +S+ SRVSSGRRSIDS SSSPLLSK S S+RSTTPIMFSNSS
Sbjct: 121 NAGSTSSSLFRSMSRRSNESMTSRVSSGRRSIDSISSSPLLSK-SGSKRSTTPIMFSNSS 180

Query: 181 GLLKPAPIEKQLECTLEELCFGCMKKIKVTRDVLSRTGQAREEEETLTIKVKPGWKKGTK 240
           G+LK A IEKQLECTLEELCFGC+KKIKVTRD+L   GQA EEEETLT+KVKPGW+KGTK
Sbjct: 181 GVLKAAAIEKQLECTLEELCFGCIKKIKVTRDLLLINGQAMEEEETLTMKVKPGWRKGTK 240

Query: 241 ITFE-GMGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISVPLL 300
           ITFE GMGNER GSYPAD SFVIAEKRHSYFKREGDDLEL VEIPL+KALTGCTISVPLL
Sbjct: 241 ITFEGGMGNERAGSYPADTSFVIAEKRHSYFKREGDDLELMVEIPLLKALTGCTISVPLL 300

Query: 301 GGEAMSLEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQRSDVCR 360
           GGE MSL+IH+VV PGY+K+I+GQGMPK KD + RG+L +KF V+FPTQLT QQRSDVCR
Sbjct: 301 GGETMSLDIHEVVSPGYEKLIQGQGMPKLKDPDTRGDLILKFFVDFPTQLTPQQRSDVCR 360

Query: 361 ILDASDYS 365
           IL+ S +S
Sbjct: 361 ILEGSHHS 364

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BLAST of Spg016212 vs. ExPASy Swiss-Prot
Match: P59910 (DnaJ homolog subfamily B member 13 OS=Homo sapiens OX=9606 GN=DNAJB13 PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.2e-37
Identity = 78/176 (44.32%), Postives = 118/176 (67.05%), Query Frame = 0


Query: 178 GLLKPAP-IEKQLECTLEELCFGCMKKIKVTRDVLSRTGQARE-EEETLTIKVKPGWKKG 237
           G+ K  P +E+ L  +LE+L FGC KKIK++R VL+  G +   +++ LTI VKPGW++G
Sbjct: 132 GVKKQDPQVERDLYLSLEDLFFGCTKKIKISRRVLNEDGYSSTIKDKILTIDVKPGWRQG 191

Query: 238 TKITFEGMGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISVPL 297
           T+ITFE  G++ P   PAD+ F++ EK H  F+RE D+L     IPL KALT CT+ V  
Sbjct: 192 TRITFEKEGDQGPNIIPADIIFIVKEKLHPRFRRENDNLFFVNPIPLGKALTCCTVEVRT 251

Query: 298 LGGEAMSLEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQR 352
           L    +++ I+D++ P Y K + G+GMP P+D   +G+L + F ++FPT+LT Q++
Sbjct: 252 LDDRLLNIPINDIIHPKYFKKVPGEGMPLPEDPTKKGDLFIFFDIQFPTRLTPQKK 307

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BLAST of Spg016212 vs. ExPASy Swiss-Prot
Match: Q80Y75 (DnaJ homolog subfamily B member 13 OS=Mus musculus OX=10090 GN=Dnajb13 PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.2e-37
Identity = 76/169 (44.97%), Postives = 115/169 (68.05%), Query Frame = 0


Query: 184 PIEKQLECTLEELCFGCMKKIKVTRDVLSRTGQARE-EEETLTIKVKPGWKKGTKITFEG 243
           PIE+ L  +LE+L FGC KKIK++R VL+    +   +++ LTI V+PGW++GT+ITFE 
Sbjct: 139 PIERDLYLSLEDLFFGCTKKIKISRRVLNEDRYSSTIKDKILTIDVRPGWRQGTRITFEK 198

Query: 244 MGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISVPLLGGEAMS 303
            G++ P   PAD+ F++ EK H  F+RE D+L     IPL KALT CT+ V  L    ++
Sbjct: 199 EGDQGPNIIPADIIFIVKEKLHPRFRREHDNLFFVYPIPLGKALTCCTVEVKTLDDRLLN 258

Query: 304 LEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQR 352
           + I+D+V P Y KI+ G+GMP P++   +G+L + F ++FPT+LT Q++
Sbjct: 259 IPINDIVHPKYFKIVPGEGMPLPENPSKKGDLFIFFDIQFPTRLTPQKK 307

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BLAST of Spg016212 vs. ExPASy Swiss-Prot
Match: Q9UDY4 (DnaJ homolog subfamily B member 4 OS=Homo sapiens OX=9606 GN=DNAJB4 PE=1 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.4e-36
Identity = 76/186 (40.86%), Postives = 122/186 (65.59%), Query Frame = 0


Query: 177 SGLLKPAPIEKQLECTLEELCFGCMKKIKVTRDVLSRTGQA-REEEETLTIKVKPGWKKG 236
           S L +  P+  +L  +LEE+  GC K++K++R  L+  G++ R E++ LTI++K GWK+G
Sbjct: 152 SRLKQDPPVIHELRVSLEEIYSGCTKRMKISRKRLNADGRSYRSEDKILTIEIKKGWKEG 211

Query: 237 TKITFEGMGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISVPL 296
           TKITF   G+E P S PAD+ F+I +K H  FKR+G ++  T +I L +AL GC+I+VP 
Sbjct: 212 TKITFPREGDETPNSIPADIVFIIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPT 271

Query: 297 LGGEAMSLEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQRSDVC 356
           L G  + + ++D+V PG ++ I G G+P PK+ + RG+L ++F V FP  ++   +  + 
Sbjct: 272 LDGRNIPMSVNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTISSSSKEVLR 331

Query: 357 RILDAS 362
           + L AS
Sbjct: 332 KHLPAS 337

[Close]

BLAST of Spg016212 vs. ExPASy Swiss-Prot
Match: Q5R8J8 (DnaJ homolog subfamily B member 4 OS=Pongo abelii OX=9601 GN=DNAJB4 PE=2 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.4e-36
Identity = 76/186 (40.86%), Postives = 122/186 (65.59%), Query Frame = 0


Query: 177 SGLLKPAPIEKQLECTLEELCFGCMKKIKVTRDVLSRTGQA-REEEETLTIKVKPGWKKG 236
           S L +  P+  +L  +LEE+  GC K++K++R  L+  G++ R E++ LTI++K GWK+G
Sbjct: 152 SRLKQDPPVIHELRVSLEEIYSGCTKRMKISRKRLNADGRSYRSEDKILTIEIKKGWKEG 211

Query: 237 TKITFEGMGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISVPL 296
           TKITF   G+E P S PAD+ F+I +K H  FKR+G ++  T +I L +AL GC+I+VP 
Sbjct: 212 TKITFPREGDETPNSIPADIVFIIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPT 271

Query: 297 LGGEAMSLEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQRSDVC 356
           L G  + + ++D+V PG ++ I G G+P PK+ + RG+L ++F V FP  ++   +  + 
Sbjct: 272 LDGRNIPMSVNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTISSSSKEVLR 331

Query: 357 RILDAS 362
           + L AS
Sbjct: 332 KHLPAS 337

[Close]

BLAST of Spg016212 vs. ExPASy Swiss-Prot
Match: Q2KIT4 (DnaJ homolog subfamily B member 4 OS=Bos taurus OX=9913 GN=DNAJB4 PE=2 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 3.1e-36
Identity = 77/186 (41.40%), Postives = 122/186 (65.59%), Query Frame = 0


Query: 177 SGLLKPAPIEKQLECTLEELCFGCMKKIKVTRDVLSRTGQA-REEEETLTIKVKPGWKKG 236
           S L +  P+  +L  +LEE+  GC K++K++R  L+  G++ R E++ LTI++K GWK+G
Sbjct: 152 SRLKQDPPVIHELRVSLEEIYSGCTKRMKISRKRLNPDGRSYRTEDKILTIEIKKGWKEG 211

Query: 237 TKITFEGMGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISVPL 296
           TKITF   G+E P S PAD+ FVI +K H  FKR+G ++  T +I L +AL GC+I+VP 
Sbjct: 212 TKITFPREGDETPTSIPADIVFVIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPT 271

Query: 297 LGGEAMSLEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQRSDVC 356
           + G  + + I+D+V PG ++ I G G+P PK+ + RG+L ++F V FP  ++   +  + 
Sbjct: 272 MDGRTIPMTINDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTISSSSKEVLR 331

Query: 357 RILDAS 362
           + L AS
Sbjct: 332 KHLPAS 337

[Close]

BLAST of Spg016212 vs. ExPASy TrEMBL
Match: A0A1S3BAK8 (chaperone protein DnaJ OS=Cucumis melo OX=3656 GN=LOC103487597 PE=4 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 2.4e-148
Identity = 297/370 (80.27%), Postives = 321/370 (86.76%), Query Frame = 0


Query: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNNHSKKDSD-VVDPKH-DASSDAIDNNGP 60
           MVDLPQALGISFRNFCKSYKSFFKKWHSKVKN  SKKD+D VVD KH DA+SDAI N+ P
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKN--SKKDTDNVVDTKHDDATSDAIHNHSP 60

Query: 61  SKDREDVNSK-GSRPTTTKGVPTSFGYGGQSMRENDRTSYRPRSYDSGFSSLSRNASRKG 120
           SKD E++  K G RPTTT     SF YGG+S+REND TS+RPRSYDSG+S+LSRNASR+G
Sbjct: 61  SKDHEEIKKKEGPRPTTTTRGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG 120

Query: 121 QNLRSSSSSQFRSVS-RSTDSVPSRVSSGRRSIDSTSSSPLLSKSSSSRRSTTPIMFSNS 180
           QN  S+SSS FRSVS RS +S+PSRVSSGRRSIDS SSSP+LSK S SRRSTTPIMFSNS
Sbjct: 121 QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPVLSK-SGSRRSTTPIMFSNS 180

Query: 181 SGLLKPAPIEKQLECTLEELCFGCMKKIKVTRDVLSRTGQAREEEETLTIKVKPGWKKGT 240
           SG+LK APIEKQLECTLEELCFGCMKKIKVTRD+L   GQA EEEETLTIKVKPGW+KGT
Sbjct: 181 SGVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLINGQAMEEEETLTIKVKPGWRKGT 240

Query: 241 KITFE-GMGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISVPL 300
           KITFE G GNER GSYPAD SFVIAEKRHSYFKREGDDLEL VEIPL+KALTGCTI VPL
Sbjct: 241 KITFEGGTGNERAGSYPADTSFVIAEKRHSYFKREGDDLELMVEIPLLKALTGCTIPVPL 300

Query: 301 LGGEAMSLEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQRSDVC 360
           LGGE MSLEIH+VV PGY+K+I+GQGMPK KD  NRGNL VKF V FPTQLT QQRSDVC
Sbjct: 301 LGGETMSLEIHEVVSPGYEKLIQGQGMPKLKDPNNRGNLIVKFFVHFPTQLTPQQRSDVC 360

Query: 361 RILDASDYSS 366
           RIL+AS Y S
Sbjct: 361 RILEASHYPS 367

[Close]

BLAST of Spg016212 vs. ExPASy TrEMBL
Match: A0A6J1BSE9 (uncharacterized protein LOC111005118 OS=Momordica charantia OX=3673 GN=LOC111005118 PE=4 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 8.8e-143
Identity = 295/366 (80.60%), Postives = 316/366 (86.34%), Query Frame = 0


Query: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNNHSKKDSDVVDPKHDASSDAIDNNGPSK 60
           MVDLPQA+GISFRNFCKSYKSFFKKWHSK+K+  SKKDSD VD K + SSDAID  GPSK
Sbjct: 1   MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKS--SKKDSD-VDQKREGSSDAID--GPSK 60

Query: 61  DREDVNSKG---SRPTTTKGVPTSFGYGGQSMREN-DRTSYRPRSYDSGFSSLSRNASRK 120
           DREDV  +G   +  T TKGVP SF YGG SMREN D  S+RPRSYDSGF SLSRNASRK
Sbjct: 61  DREDVKREGRATATATATKGVP-SFRYGG-SMRENHDPLSFRPRSYDSGF-SLSRNASRK 120

Query: 121 GQNLRSSSSSQFRSVSR--STDSVPSRVSSGRRSIDSTSSSPLLSKSSSSRRSTTPIMFS 180
            QN   SSS  FRSVSR  +  SVPSRVSSGRRS+D  SS PLLSK S SRRSTTPIMFS
Sbjct: 121 SQNTH-SSSPLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSK-SGSRRSTTPIMFS 180

Query: 181 NSSGLLKPAPIEKQLECTLEELCFGCMKKIKVTRDVLSR-TGQAREEEETLTIKVKPGWK 240
           NSSG+LKPAPIEKQLECTLEELCFGC KKIK+TRDVLS  TGQ  EEEETLTI VKPGWK
Sbjct: 181 NSSGVLKPAPIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWK 240

Query: 241 KGTKITFEGMGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISV 300
           KGTKITFEGMGNERPGSYPADMSFVIAEKRH++FK EGDDLE+TVEIPL+KALTGCTISV
Sbjct: 241 KGTKITFEGMGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISV 300

Query: 301 PLLGGEAMSLEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQRSD 360
           PLLGGEAMSLEI DVV PGY++II+GQGMP PKDD+ RG+LKVKFLVEFPTQLT QQRSD
Sbjct: 301 PLLGGEAMSLEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSD 356

[Close]

BLAST of Spg016212 vs. ExPASy TrEMBL
Match: A0A6J1FLF5 (dnaJ homolog subfamily B member 5-like OS=Cucurbita moschata OX=3662 GN=LOC111446483 PE=4 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 8.8e-143
Identity = 281/367 (76.57%), Postives = 314/367 (85.56%), Query Frame = 0


Query: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNNHSKKDSDVVDPKHDASSDAID--NNGP 60
           MVDLPQ LG+SFRNFCKSYKSFFKKWHSKVKN  SKK++DVVD KHD SSDAID  NNGP
Sbjct: 1   MVDLPQVLGVSFRNFCKSYKSFFKKWHSKVKN--SKKNTDVVDSKHDGSSDAIDNNNNGP 60

Query: 61  SKDREDVNSKGSRPTTTKGVPTSFGYGGQSMRENDRTSYRPRSYDSGFSSLSRNASRKGQ 120
           SKDRE++N +G R  T KG   SF Y  +SMREND    R RSYD   SS+ RNASR+G 
Sbjct: 61  SKDREEINKEGPRQMTKKG-GLSFRYASRSMRENDPKISRHRSYD---SSMFRNASRRGL 120

Query: 121 NLRSSSSSQFRSVS-RSTDSVPSRVSSGRRSIDSTSSSPLLSKSSSSRRSTTPIMFSNSS 180
           N + SSSSQFRSVS RST+S+PSR++SG  S+DS SSSP+LSK S SRRSTTPIMFSNSS
Sbjct: 121 NPQPSSSSQFRSVSRRSTESMPSRLNSG-LSVDSASSSPMLSK-SGSRRSTTPIMFSNSS 180

Query: 181 GLLKPAPIEKQLECTLEELCFGCMKKIKVTRDVLSRTGQAREEEETLTIKVKPGWKKGTK 240
           G+LKP PIEKQLECTLEELCFGCMKKIKVTRDV+  TGQA EEEET+TI+VKPGW KGTK
Sbjct: 181 GVLKPPPIEKQLECTLEELCFGCMKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTK 240

Query: 241 ITFEGMGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISVPLLG 300
           ITFEGMGNERPGSYPAD+SFVI EK+H YFKREGDDL+LTVEIPL+KALTGCTIS+PLLG
Sbjct: 241 ITFEGMGNERPGSYPADISFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLG 300

Query: 301 GEAMSLEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQRSDVCRI 360
           G+ MSL  HDV+W G  KIIKGQGMPK K++ NRGNLKVKFL+EFPT+LT QQRSDVCRI
Sbjct: 301 GDVMSLRTHDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRI 359

Query: 361 LDASDYS 365
           L+ S+YS
Sbjct: 361 LEGSNYS 359

[Close]

BLAST of Spg016212 vs. ExPASy TrEMBL
Match: A0A0A0KYJ4 (DnaJ_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G361870 PE=4 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 1.2e-142
Identity = 285/368 (77.45%), Postives = 318/368 (86.41%), Query Frame = 0


Query: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNNHSKKDSDVVDPKH-DASSDAIDNNGPS 60
           MVDLPQALGISFRNFCKSYKSFFKKWHSKVKN+    D+ VVD KH D +SDAI N GPS
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDPTSDAIHNYGPS 60

Query: 61  KDREDVNSK-GSRPTTTKGVPTSFGYGGQSMRENDRTSYRPRSYDSGFSSLSRNASRKGQ 120
           KD ED+  K G RP  TKGV  SF YGG+S+REND TS+RP+SYDSG+S+LSRNASR+GQ
Sbjct: 61  KDHEDIKKKEGPRP--TKGV-HSFRYGGRSLRENDTTSFRPQSYDSGYSTLSRNASRRGQ 120

Query: 121 NLRSSSSSQFRSVS-RSTDSVPSRVSSGRRSIDSTSSSPLLSKSSSSRRSTTPIMFSNSS 180
           N  S+SSS FRS+S RS +S+ SRVSSGRRSIDS SSSPLLSK S S+RSTTPIMFSNSS
Sbjct: 121 NAGSTSSSLFRSMSRRSNESMTSRVSSGRRSIDSISSSPLLSK-SGSKRSTTPIMFSNSS 180

Query: 181 GLLKPAPIEKQLECTLEELCFGCMKKIKVTRDVLSRTGQAREEEETLTIKVKPGWKKGTK 240
           G+LK A IEKQLECTLEELCFGC+KKIKVTRD+L   GQA EEEETLT+KVKPGW+KGTK
Sbjct: 181 GVLKAAAIEKQLECTLEELCFGCIKKIKVTRDLLLINGQAMEEEETLTMKVKPGWRKGTK 240

Query: 241 ITFE-GMGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISVPLL 300
           ITFE GMGNER GSYPAD SFVIAEKRHSYFKREGDDLEL VEIPL+KALTGCTISVPLL
Sbjct: 241 ITFEGGMGNERAGSYPADTSFVIAEKRHSYFKREGDDLELMVEIPLLKALTGCTISVPLL 300

Query: 301 GGEAMSLEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQRSDVCR 360
           GGE MSL+IH+VV PGY+K+I+GQGMPK KD + RG+L +KF V+FPTQLT QQRSDVCR
Sbjct: 301 GGETMSLDIHEVVSPGYEKLIQGQGMPKLKDPDTRGDLILKFFVDFPTQLTPQQRSDVCR 360

Query: 361 ILDASDYS 365
           IL+ S +S
Sbjct: 361 ILEGSHHS 364

[Close]

BLAST of Spg016212 vs. ExPASy TrEMBL
Match: A0A6J1ISU0 (dnaJ homolog subfamily B member 1-like OS=Cucurbita maxima OX=3661 GN=LOC111480292 PE=4 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 4.8e-141
Identity = 280/367 (76.29%), Postives = 314/367 (85.56%), Query Frame = 0


Query: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNNHSKKDSDVVDPKHDASSDAID--NNGP 60
           MVDLPQ LG+SFRNFCKSYKSFFKKWHSKVKN  SKK++DVVD KHD SSDAID  NNGP
Sbjct: 1   MVDLPQVLGVSFRNFCKSYKSFFKKWHSKVKN--SKKNTDVVDSKHDGSSDAIDNNNNGP 60

Query: 61  SKDREDVNSKGSRPTTTKGVPTSFGYGGQSMRENDRTSYRPRSYDSGFSSLSRNASRKGQ 120
           SKDRE+++ +G R  T KG   SF YG +SMREND    R RSYD   SS+ RNASR+G 
Sbjct: 61  SKDREEISKEGPRQMTKKG-GLSFRYGSRSMRENDPKISRHRSYD---SSMFRNASRRGL 120

Query: 121 NLRSSSSSQFRSVS-RSTDSVPSRVSSGRRSIDSTSSSPLLSKSSSSRRSTTPIMFSNSS 180
           N + SSSSQFRSVS RST+S+PSR++SG  S+DS SSSP+LSK S SRRSTTPIMFSNSS
Sbjct: 121 NPQPSSSSQFRSVSRRSTESMPSRLNSG-LSVDSASSSPMLSK-SGSRRSTTPIMFSNSS 180

Query: 181 GLLKPAPIEKQLECTLEELCFGCMKKIKVTRDVLSRTGQAREEEETLTIKVKPGWKKGTK 240
           G+LKPAPIEKQLECTLEELCFGCMKKIKVTRDV+  TGQA EEEET+TI+VKPGW KGTK
Sbjct: 181 GVLKPAPIEKQLECTLEELCFGCMKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTK 240

Query: 241 ITFEGMGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISVPLLG 300
           ITFEGMGNER GSYPAD+SFVI EK+H YFKREGDDL+LTVEIPLVKALTGCTIS+PLLG
Sbjct: 241 ITFEGMGNERLGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLVKALTGCTISIPLLG 300

Query: 301 GEAMSLEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQRSDVCRI 360
           G+ MSL+  DV+W G  KIIKGQGMPK K++ NRGNLKVKFL EFPT+LT QQRSD+CRI
Sbjct: 301 GDIMSLKTQDVIWHGCVKIIKGQGMPKLKEEINRGNLKVKFLTEFPTELTDQQRSDICRI 359

Query: 361 LDASDYS 365
           L+ S+YS
Sbjct: 361 LEGSNYS 359

[Close]

BLAST of Spg016212 vs. TAIR 10
Match: AT1G11040.1 (HSP40/DnaJ peptide-binding protein )

HSP 1 Score: 243.8 bits (621), Expect = 2.1e-64
Identity = 129/252 (51.19%), Postives = 179/252 (71.03%), Query Frame = 0


Query: 107 SSLSRNASRKGQNLRSSSSSQFRSVSRSTDSVPSRVSSGRRSIDSTSSSPLLSKSSSSRR 166
           SS+S++AS++ ++ R S+SS     + ST    S+  S R +  S +        S SRR
Sbjct: 193 SSISKSASKRDKDERGSTSS-----ATSTSLPYSKSKSTRDTAGSIA-------KSISRR 252

Query: 167 STTPIMFSNSSGLLKPAPIEKQLECTLEELCFGCMKKIKVTRDVLSRTGQAREEEETLTI 226
           STTPI+FS S+   KP  +EK+LECTLEELC G +K IK+ RD+++  G   ++EE L +
Sbjct: 253 STTPIVFSQSTPPKKPPAVEKKLECTLEELCHGGVKNIKIKRDIITDEGLIMQQEEMLRV 312

Query: 227 KVKPGWKKGTKITFEGMGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKAL 286
            ++PGWKKGTKITFEG+GNE+PG  P D++FV+ EKRH  FKR GDDLE+ VEIPL+KAL
Sbjct: 313 NIQPGWKKGTKITFEGVGNEKPGYLPEDITFVVEEKRHPLFKRRGDDLEIAVEIPLLKAL 372

Query: 287 TGCTISVPLLGGEAMSLEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQL 346
           TGC +SVPLL GE+MS+ + DV++ G++K IKGQGMP  K++  RG+L++ FLV FP +L
Sbjct: 373 TGCKLSVPLLSGESMSITVGDVIFHGFEKAIKGQGMPNAKEEGKRGDLRITFLVNFPEKL 432

Query: 347 THQQRSDVCRIL 359
           + +QRS    +L
Sbjct: 433 SEEQRSMAYEVL 432

[Close]

BLAST of Spg016212 vs. TAIR 10
Match: AT1G44160.1 (HSP40/DnaJ peptide-binding protein )

HSP 1 Score: 224.9 bits (572), Expect = 1.0e-58
Identity = 128/270 (47.41%), Postives = 177/270 (65.56%), Query Frame = 0


Query: 96  SYRPRSYDSGFSSLSRNASRK-GQNLRSSSSSQFRSVSRSTDSVPSRVSSGRRSIDSTSS 155
           SYR  + ++ F    ++ SR   QN  +++S+      RS   +    SS  R  +S   
Sbjct: 89  SYRFDNTNTNFVGSKKSLSRSCSQNTATATST--NPTLRSLSFIGRSKSSSNRMTESGGF 148

Query: 156 SPLLSKSSSS--RRSTTPIMFSNSSG-LLKPAPIEKQLECTLEELCFGCMKKIKVTRDVL 215
            P L +S+++  R    PI++S+SS  + KP+P EK+L CTLEELC GC KKIK+ RDV+
Sbjct: 149 MPTLMRSTTTVPRSFANPILYSSSSAKVAKPSPTEKKLRCTLEELCNGCTKKIKIKRDVI 208

Query: 216 SRTGQAREEEETLTIKVKPGWKKGTKITFEGMGNERPGSYPADMSFVIAEKRHSYFKREG 275
           +  G+  EEEE + IKVKPGWK GTK+TFEG GNE   S PAD++FVI EK H  FKREG
Sbjct: 209 TSLGEKCEEEEMVEIKVKPGWKGGTKVTFEGKGNEAMRSVPADLTFVIVEKEHEVFKREG 268

Query: 276 DDLELTVEIPLVKALTGCTISVPLLGGEAMSLEIHDVVWPGYQKIIKGQGMPKPKDDENR 335
           DDLE+ VE+ L++ALTGC +SV LL G+ M L I DV+ PGY  +++G+GMP  K+   R
Sbjct: 269 DDLEMAVEVSLLEALTGCELSVALLDGDNMRLRIEDVIHPGYVTVVQGKGMPNLKEKGKR 328

Query: 336 GNLKVKFLVEFPTQLTHQQRSDVCRILDAS 362
           G+L+V+F  +FP  LT +QR+++  IL  S
Sbjct: 329 GDLRVRFRTKFPQHLTDEQRAEIHSILQDS 356

[Close]

BLAST of Spg016212 vs. TAIR 10
Match: AT3G08910.1 (DNAJ heat shock family protein )

HSP 1 Score: 192.6 bits (488), Expect = 5.6e-49
Identity = 130/346 (37.57%), Postives = 190/346 (54.91%), Query Frame = 0


Query: 17  KSYKSFFKKWHSKVKNNHSKKDSDVVDPKHDASSDAIDNNGPSKDREDVNSKGSRPTTTK 76
           K+Y+    KWH   KN ++KKD++    K    S+A D     + R   +  G    T++
Sbjct: 22  KAYRKLAMKWHPD-KNPNNKKDAEA---KFKQISEAYDVLSDPQKRAIYDQYGEEGLTSQ 81

Query: 77  GVPTSFGYGGQSMRENDRTSYRPRSYDSGFSSLSRNASRKGQNLRSSSSSQFRSVSRSTD 136
             P   G GG                  GF               S   + FR   RS D
Sbjct: 82  APPP--GAGG------------------GF---------------SDGGASFRFNGRSAD 141

Query: 137 SVPSRVSSGRRSI-DSTSSSPLLSKSSSSRRSTTPIMFSNSSGLLKPAPIEKQLECTLEE 196
            + S      R   DS  + P     S+  R    +  SN     K APIE+QL C+LE+
Sbjct: 142 DIFSEFFGFTRPFGDSRGAGP-----SNGFRFAEDVFSSNVVPPRKAAPIERQLPCSLED 201

Query: 197 LCFGCMKKIKVTRDVLSRTGQAREEEETLTIKVKPGWKKGTKITFEGMGNERPGSYPADM 256
           L  G  KK+K++RDVL  +G+    EE LTI++KPGWKKGTKITF   GNE+ G  P+D+
Sbjct: 202 LYKGVSKKMKISRDVLDSSGRPTTVEEILTIEIKPGWKKGTKITFPEKGNEQRGIIPSDL 261

Query: 257 SFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISVPLLGGEAMSLEIHDVVWPGYQK 316
            F++ EK H+ FKR+G+DL +T +IPLV+ALTG T  V  L G ++++ I++V+ P Y++
Sbjct: 262 VFIVDEKPHAVFKRDGNDLVMTQKIPLVEALTGYTAQVSTLDGRSVTVPINNVISPSYEE 321

Query: 317 IIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQRSDVCRILDAS 362
           ++KG+GMP PKD   +GNL++KF V+FP++LT +Q+S + R+  +S
Sbjct: 322 VVKGEGMPIPKDPSKKGNLRIKFTVKFPSRLTTEQKSGIKRMFSSS 323

[Close]

BLAST of Spg016212 vs. TAIR 10
Match: AT5G01390.1 (DNAJ heat shock family protein )

HSP 1 Score: 177.6 bits (449), Expect = 1.9e-44
Identity = 120/345 (34.78%), Postives = 184/345 (53.33%), Query Frame = 0


Query: 17  KSYKSFFKKWHSKVKNNHSKKDSDVVDPKHDASSDAIDNNGPSKDREDVNSKGSRPTTTK 76
           K+Y+    KWH   KN ++KK+++    +   + D + +       E    +G       
Sbjct: 22  KAYRKLAMKWHPD-KNPNNKKEAEAKFKQISEAYDVLSDPQKRAIYEQYGEEGLNQAPPP 81

Query: 77  GVPTSFGYGGQSMRENDRTSYRPRSYD---SGFSSLSRNASRKGQNLRSSSSSQFRSVSR 136
           G    +  GG     + R  + PRS D   S F   +R +   G + R+  S  FR    
Sbjct: 82  GAGGGYP-GGSDAGASFR--FNPRSADDIFSEFFGFTRPSFGTGSDSRAGPSG-FRYGDD 141

Query: 137 STDSVPSRVSSGRRSIDSTSSSPLLSKSSSSRRSTTPIMFSNSSGLLKPAPIEKQLECTL 196
              S  +  + G  SI S                             K APIE+QL C+L
Sbjct: 142 IFASFRAATTGGEASIPSR----------------------------KSAPIERQLPCSL 201

Query: 197 EELCFGCMKKIKVTRDVLSRTGQAREEEETLTIKVKPGWKKGTKITFEGMGNERPGSYPA 256
           E+L  G  KK+K++RDVL  +G+    EE LTI++KPGWKKGTKITF   GNE  G  P+
Sbjct: 202 EDLYKGVSKKMKISRDVLDSSGRPTPVEEILTIEIKPGWKKGTKITFLEKGNEHRGVIPS 261

Query: 257 DMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISVPLLGGEAMSLEIHDVVWPGY 316
           D+ F++ EK H  FKR+G+DL +  +I LV ALTG T  V  L G  +++ +++V+ P Y
Sbjct: 262 DLVFIVDEKPHPVFKRDGNDLVVMQKISLVDALTGYTAQVTTLDGRTLTVPVNNVISPSY 321

Query: 317 QKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQRSDVCRIL 359
           ++++KG+GMP PKD   +GNL+++F+++FP++LT +Q+S + R+L
Sbjct: 322 EEVVKGEGMPIPKDPSRKGNLRIRFIIKFPSKLTTEQKSGIKRML 333

[Close]

BLAST of Spg016212 vs. TAIR 10
Match: AT2G20560.1 (DNAJ heat shock family protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.7e-42
Identity = 125/358 (34.92%), Postives = 180/358 (50.28%), Query Frame = 0


Query: 17  KSYKSFFKKWHSKVKNNHSKKDSDVV------------DPKHDASSDAIDNNGPSKDRED 76
           K+Y+    KWH   KN ++KKD++ +            DP+  A  D     G       
Sbjct: 22  KAYRKLAMKWHPD-KNPNNKKDAEAMFKQISEAYEVLSDPQKKAVYDQYGEEG------- 81

Query: 77  VNSKGSRPTTTKGVPTSF--GYGGQSMRENDRTSYRPRSYDSGFSSLSRNASRKGQNLRS 136
              KG+ P    G  T F  G G  S R N R +    +   GFSS      R G    S
Sbjct: 82  --LKGNVPPPDAGGATYFSTGDGPTSFRFNPRNADDIFAEFFGFSS-PFGGGRGGTRFSS 141

Query: 137 S--SSSQFRSVSRSTDSVPSRVSSGRRSIDSTSSSPLLSKSSSSRRSTTPIMFSNSSGLL 196
           S    + F S           +  G                                G  
Sbjct: 142 SMFGDNMFASFGEGGGGGGGSMHHG--------------------------------GAR 201

Query: 197 KPAPIEKQLECTLEELCFGCMKKIKVTRDVLSRTGQAREEEETLTIKVKPGWKKGTKITF 256
           K APIE +L C+LE+L  G  KK++++R++   +G+  + EE LTI VKPGWKKGTKITF
Sbjct: 202 KAAPIENKLPCSLEDLYKGTTKKMRISREIADVSGKTMQVEEILTIDVKPGWKKGTKITF 261

Query: 257 EGMGNERPGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLVKALTGCTISVPLLGGEA 316
              GNE+PG  PAD+ F+I EK H  F REG+DL +T +I LV+ALTG T+++  L G  
Sbjct: 262 PEKGNEQPGVIPADLVFIIDEKPHPVFTREGNDLIVTQKISLVEALTGYTVNLTTLDGRR 321

Query: 317 MSLEIHDVVWPGYQKIIKGQGMPKPKDDENRGNLKVKFLVEFPTQLTHQQRSDVCRIL 359
           +++ + +VV P Y++++  +GMP  KD   RGNL++KF ++FPT+LT +Q++ V ++L
Sbjct: 322 LTIPVTNVVHPEYEEVVPKEGMPLQKDQTKRGNLRIKFNIKFPTRLTSEQKTGVKKLL 336

The following BLAST results are available for this feature:

BLAST of Spg016212 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899191.1	3.2e-155	83.01	dnaJ homolog subfamily B member 4-like [Benincasa hispida]	[more]
XP_008444178.1	4.9e-148	80.27	PREDICTED: chaperone protein DnaJ [Cucumis melo]	[more]
XP_022941087.1	1.8e-142	76.57	dnaJ homolog subfamily B member 5-like [Cucurbita moschata]	[more]
XP_022132204.1	1.8e-142	80.60	uncharacterized protein LOC111005118 [Momordica charantia]	[more]
XP_004142703.1	2.4e-142	77.45	uncharacterized protein LOC101218142 [Cucumis sativus] >KGN54553.1 hypothetical ...	[more]

BLAST of Spg016212 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P59910	2.2e-37	44.32	DnaJ homolog subfamily B member 13 OS=Homo sapiens OX=9606 GN=DNAJB13 PE=1 SV=1	[more]
Q80Y75	2.2e-37	44.97	DnaJ homolog subfamily B member 13 OS=Mus musculus OX=10090 GN=Dnajb13 PE=1 SV=1	[more]
Q9UDY4	1.4e-36	40.86	DnaJ homolog subfamily B member 4 OS=Homo sapiens OX=9606 GN=DNAJB4 PE=1 SV=1	[more]
Q5R8J8	1.4e-36	40.86	DnaJ homolog subfamily B member 4 OS=Pongo abelii OX=9601 GN=DNAJB4 PE=2 SV=1	[more]
Q2KIT4	3.1e-36	41.40	DnaJ homolog subfamily B member 4 OS=Bos taurus OX=9913 GN=DNAJB4 PE=2 SV=1	[more]

BLAST of Spg016212 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BAK8	2.4e-148	80.27	chaperone protein DnaJ OS=Cucumis melo OX=3656 GN=LOC103487597 PE=4 SV=1	[more]
A0A6J1BSE9	8.8e-143	80.60	uncharacterized protein LOC111005118 OS=Momordica charantia OX=3673 GN=LOC111005...	[more]
A0A6J1FLF5	8.8e-143	76.57	dnaJ homolog subfamily B member 5-like OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A0A0KYJ4	1.2e-142	77.45	DnaJ_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G361870 PE=4...	[more]
A0A6J1ISU0	4.8e-141	76.29	dnaJ homolog subfamily B member 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114802...	[more]

BLAST of Spg016212 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11040.1	2.1e-64	51.19	HSP40/DnaJ peptide-binding protein	[more]
AT1G44160.1	1.0e-58	47.41	HSP40/DnaJ peptide-binding protein	[more]
AT3G08910.1	5.6e-49	37.57	DNAJ heat shock family protein	[more]
AT5G01390.1	1.9e-44	34.78	DNAJ heat shock family protein	[more]
AT2G20560.1	1.7e-42	34.92	DNAJ heat shock family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.60.260.20		coord: 184..263 e-value: 1.4E-29 score: 104.0
None	No IPR available	GENE3D	2.60.260.20		coord: 264..363 e-value: 4.0E-25 score: 89.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 33..179
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 70..92
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 33..69
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 99..176
None	No IPR available	PANTHER	PTHR24078:SF535	DNAJ HOMOLOG SUBFAMILY B MEMBER 13-LIKE	coord: 49..359
None	No IPR available	PANTHER	PTHR24078	DNAJ HOMOLOG SUBFAMILY C MEMBER	coord: 49..359
None	No IPR available	CDD	cd10747	DnaJ_C	coord: 183..346 e-value: 3.96705E-52 score: 168.373
IPR002939	Chaperone DnaJ, C-terminal	PFAM	PF01556	DnaJ_C	coord: 185..343 e-value: 8.9E-43 score: 146.1
IPR008971	HSP40/DnaJ peptide-binding	SUPERFAMILY	49493	HSP40/DnaJ peptide-binding domain	coord: 185..264
IPR008971	HSP40/DnaJ peptide-binding	SUPERFAMILY	49493	HSP40/DnaJ peptide-binding domain	coord: 266..352

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg016212.1	Spg016212.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051085	chaperone cofactor-dependent protein refolding
biological_process	GO:0006457	protein folding
cellular_component	GO:0005829	cytosol
molecular_function	GO:0051087	chaperone binding
molecular_function	GO:0051082	unfolded protein binding