Spg015195 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg015195
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRibonuclease T(2)
Locationscaffold3: 40387691 .. 40391946 (-)
RNA-Seq ExpressionSpg015195
SyntenySpg015195
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAATATTTGAGATATCCGACAATGACAAAGAAAGCAAGTGTGTTCCTCGTAAGCATATGGCTTCTAATAGCTTTGTCAAAACAAGCTGATTTCGACTATGTTCAGATGGTGTTGGAATGGCAGCCAGCCATGTGCAACCTTGGAAAGAGCTGCAAAATAGAACCAAAGAACAAGTTTAGTATTCATGGTGTGTGGCCTAGCCTCTACTCTAGGGGCCAAATTGGACCTTGCTATGGCCCTCCTTTTAACCCCGACAAGGTATCAAAACATTCTTTATTATTTATTTATTTATTACAATATGTGGGGTGGGAGGATTCAAACTATAGACCTTTTGATCATTAGTATAAGTTTTATGCCAGTTGAGTCAGACTCATGTTGGCTAAAACATTTATTATTATATATGTAAAAGTATTTTATATGTTTTATTTACTCTTTGTTGCTTCTTTGAACTCGGTTTAAGTTTTTGTCGCTCAAACTCGATGTGTGTTTGTCAGGGATGATGGTCTTAATAGCGATCTTATATATGAGATCATGTTGGCCAAGACATTTATTATTATATATGTAAAAGTATTTTATATTTTTATCTGATATACCACTTGTTAAGGGACAACTGGACACTCCACATATCTCCACAATAGTTCGATATTGTTCACTTTGGACATAAGCTCTCGGTTTTATTTTTGGATTACCCCAAAAGGGCTCATACCAATGGAGATACTTGTCCTTCCTTATATACCCATGATCATCCCTTTGTCTAGCCAGTGTGAGACTTTGGTCGCACTCCCAACATTGACATTGAAATAATGATAGTAAGCACAACCGTTGTTTTTTATTATTATTATTATTTTATTTTTTAATGAACATTTTGATAATAGATGAGTTGGATTTAGGGTTGAAGTCTAAGCTCTCTAACCTTTTTTTTTTCTTTTCTCAATATTTTTTTTTCATTTTTGCTTTTACAAAATGTTTTTATGGATTTTTTTTCTCTCTCTCTGTATAGTTTAATTTGTGAAATATATCTTCTTTACTAAATTTGAATTTATCGAAAATTAACAAATACAGAGCACATAACTATAGTTATTCAACTTGTAGACATTATTTATTAGTCTTATAATTTTGTTTCGAATTCCTCCTCCAATTAGATAAATATGTTTTCAAAAAAAAAAAAAAAAAAAAAAAATCAAGAAACTAAATAATTTTTTTTTAAAAAGTTTCCTCTAACATAAAATTCTTAATCCACCTGGTCGAGTTATTTAGATCATCGCATTGCAAGACCGAATGAACCAAAACTGGCCAGACCTAAAGGAAGGGAACTCATTGGAAGCGTGGAGGAGCAAATGGGCAATCCACGGGACATGCTCGGCGGCAGTGTTCAATCAGTTCGAATACTTTAGCTTGGGTTTGGACACCTACGGCCGCTACGCCATGTTGTCAATTCTAGACAAAGGAGGTGTGGCTCCTAGCTCTTACAGGTATATAGCCAAGACCCAGTTTATCGACGTCATCACCGAGTTCACGTCAAAGAAGGGCGGTGTCATTTGTGCCACCGACCACCGTGGCCGAAGACAGGTCCAGAAGGTCATTTTATGTTATGCCAAAGATGGTGCTACTCTCATCAACTGCCCAGAAAATGTTAGTAATTCCTGCCCTGAATACTTCATGTGGCTTGGCTTGGATGATTGAGTCATGTAATTGAAGCTAAAAAGATAATGAAGCTAATATGTAATATACTTTCTATCCTAATGAATAAAATTATGCTTTGATCTGCCTCATATTGCCTATGTAGCAAAATGAGGCAGAAGGGAAGCTTTGTGTTTACTCTCTCATGCATGCAACTTCTTATTGGTGTGATCTATTTCATAATACTTTCTTATAAGACTTGATATTAGGAATAAATGTGAATTTTAACAAAATAATATATTAGAATTAGTTGATTAATTATGGGAGCTTGTACACGCATCAAGAGTTTTAATAAAATGATGAGTAAAAGGACACTAACCTTTAAAATTAGGGTCTTCAATTCATTCGAGTACCACATGTTATATTCTTGAATTTTATTCTAAAAACAACGACTTACAATTCCTAGATAATTGATTTGACTCACAAAATTTATAAATTTAACTAATAAATTAATCAAGTTTGAATGAATTTGAATTCTATAGGTCTCAAGAACTCCATACTCTGACTTCCTCTCACGAGGTGAATCATTTCTTAATTGAACCTCCTTGATCACGAATCTTAAAATAATCCCTTTCTTTAGCTTTCAAATATTATCTTTAGCCCTCCAAAATATGCTCCAAGTTATTTATGTTGTTTCTCTCTAAGTTATTTATTTTCAAATCCCATAAAATAAAAAATAAAATATGTGTACCACATAAACTTAACTTCTTGAGGCACATGTCCCAAATTCATGGCTCGTGTTCGGGGCATCTAGTGCTAAATTGGACTTCTAGGCATCGAATTTTGTTTTATTTCATTACATTTTACATAGTTGTATAAAAGTATTTTAACGAATCTAAAATAATTAGAAAACTAACAACTAGCTAATTGATTAAAATCACCGTCTAAGAGTGTGTTTGGTTTAACTTTTCAAGTGTTTAATTCTGAAAAAAATCATTTAAAAAAATTATAGTGTTTGGCAACCACTAAAAATGACTTATTAAAAAATGAGATTAAGAAATAAACCATTTTGGGGGGAAACACTTCAATTCAAATTTTTTGAAAAGAGTTTTTTAAGTATACAATAACTTGTGTATAAAAATACTTGTGAGGAAATGTGACCAAACATGAGTGTTTAAATCCTAAACAAATTTTTTACAAAATGCTTAAAAAAAATGCATTCTTGAAAAACACTTAAACTAAAATGTATTCCAAACGAGCCCAAAATGCCAGCGAATATGAGTTGATTAGGTTTCTTAGATTTTTTTTTTTGCTAGCTCGTATACTAGATAAACTTATTTAAATGGAAAATAGTAACTCAAATAATTTACATTATATGAATTTGATTTGTTTTCTTAATATTTATTCAATAATAATCACTTCACTTAATCAACTCGTTAACGTTTCACAACTTCAATAAAAATGTTGGAGGTTTGAATCTCTATCAAACGAATTAAAAGTATATATATTAAATAATGAATATTAAAAAAAATAAATGTGGGTCATTTTTAGTTAAAAGGTTTTCAAGAAACAATTTTAAGACACTTTTTTGGTGGTGGGATGACTTCATCGTACCAGAAAACGTACGGCGTTGTTTGGAAGTAGTTTAGGAAAAATAATTAATGAATCCATGTTTGGTTGGCTTTTGGTTTAGTTGTCTTCACACTTGTTCATTTTTAAGATCATTACATATAAGATCCTTAATGTTGCTCAATTAAGTAATCATCCACTGTCTTTCTTCAATGAAAAAAAAGGTAATCATCCACTACTTCTCAAAAAAAAAAAAAAAAAAAAAAAATCATCCACTAAGACCACAATTTTATTATTACTAGGGGGAGTGTTCATCGCTACTGAATGACATACCAGTGATAACCCAAAAGTTTTCAAATATATATAGATATACAACTGTTTCTTAAAAATGAAAAAGAAGAAACAAAAAACACAGACAAAAACCCAAGTTTATTTTCGAAAACTAAAACAAGAAAACCAACCTAGAAAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAAGAACCAACATTAGAAATGTTATGTTGGGGGAATCACAAATAATAGATAAAATTGGAGTAAATGTTAAGATACAAATACATATACAATTAGACATACGTAACTGATTTCACCCTGGTCGAGTTATTTAGATCATCGCATTGCAAGACCGAATGAACCAAAACTGGCCAGACCTAAAGGAAGGGAACTCATTGGAAGCGTGGAGGAGCAAATGGGCAATCCACGGGACATGCTCGGCGGCAGTGTTCAATCAGTTCGAATACTTTAGCTTGGGTTTGGACACCTACGGCCGCTATGCCATGTTGTCAATTCTAGACAAAGGAGGTTTGGCTCCTAGCTCTTATAAGCATATAGGCAAGACCGAGTTTATCGACGTCATGGCCGAGTTCACGTCGAAGAAGGGCGGCGTCATTTGTGCCTCCGACCACCGTGGCCGAAGACAGGTCCAGAAGGTCATTTTATGTTATGCCAAAGATGGTGCTACTCTCATCAACTGCCCAGAGAATGTTAGTAATTCCTGCCCTGAATACTTCATGTGGCTTGGCTTGGATGATTGA

mRNA sequence

ATGACAAAGAAAGCAAGTGTGTTCCTCGTAAGCATATGGCTTCTAATAGCTTTGTCAAAACAAGCTGATTTCGACTATGTTCAGATGGTGTTGGAATGGCAGCCAGCCATGTGCAACCTTGGAAAGAGCTGCAAAATAGAACCAAAGAACAAGTTTAGTATTCATGGTGTGTGGCCTAGCCTCTACTCTAGGGGCCAAATTGGACCTTGCTATGGCCCTCCTTTTAACCCCGACAAGATCATCGCATTGCAAGACCGAATGAACCAAAACTGGCCAGACCTAAAGGAAGGGAACTCATTGGAAGCGTGGAGGAGCAAATGGGCAATCCACGGGACATGCTCGGCGGCAGTGTTCAATCAGTTCGAATACTTTAGCTTGGGTTTGGACACCTACGGCCGCTACGCCATGTTGTCAATTCTAGACAAAGGAGGTGTGGCTCCTAGCTCTTACAGGTATATAGCCAAGACCCAGTTTATCGACGTCATCACCGAGTTCACGTCAAAGAAGGGCGGTGTCATTTGTGCCACCGACCACCGTGGCCGAAGACAGGTCCAGAAGGTCATTTTATGTTATGCCAAAGATGGTGCTACTCTCATCAACTGCCCAGAAAATGTTAGTAATTCCTGCCCTGAATACTTCATGTCTCAAGAACTCCATACTCTGACTTCCTCTCACGAGATCATCGCATTGCAAGACCGAATGAACCAAAACTGGCCAGACCTAAAGGAAGGGAACTCATTGGAAGCGTGGAGGAGCAAATGGGCAATCCACGGGACATGCTCGGCGGCAGTGTTCAATCAGTTCGAATACTTTAGCTTGGGTTTGGACACCTACGGCCGCTATGCCATGTTGTCAATTCTAGACAAAGGAGGTTTGGCTCCTAGCTCTTATAAGCATATAGGCAAGACCGAGTTTATCGACGTCATGGCCGAGTTCACGTCGAAGAAGGGCGGCGTCATTTGTGCCTCCGACCACCGTGGCCGAAGACAGGTCCAGAAGGTCATTTTATGTTATGCCAAAGATGGTGCTACTCTCATCAACTGCCCAGAGAATGTTAGTAATTCCTGCCCTGAATACTTCATGTGGCTTGGCTTGGATGATTGA

Coding sequence (CDS)

ATGACAAAGAAAGCAAGTGTGTTCCTCGTAAGCATATGGCTTCTAATAGCTTTGTCAAAACAAGCTGATTTCGACTATGTTCAGATGGTGTTGGAATGGCAGCCAGCCATGTGCAACCTTGGAAAGAGCTGCAAAATAGAACCAAAGAACAAGTTTAGTATTCATGGTGTGTGGCCTAGCCTCTACTCTAGGGGCCAAATTGGACCTTGCTATGGCCCTCCTTTTAACCCCGACAAGATCATCGCATTGCAAGACCGAATGAACCAAAACTGGCCAGACCTAAAGGAAGGGAACTCATTGGAAGCGTGGAGGAGCAAATGGGCAATCCACGGGACATGCTCGGCGGCAGTGTTCAATCAGTTCGAATACTTTAGCTTGGGTTTGGACACCTACGGCCGCTACGCCATGTTGTCAATTCTAGACAAAGGAGGTGTGGCTCCTAGCTCTTACAGGTATATAGCCAAGACCCAGTTTATCGACGTCATCACCGAGTTCACGTCAAAGAAGGGCGGTGTCATTTGTGCCACCGACCACCGTGGCCGAAGACAGGTCCAGAAGGTCATTTTATGTTATGCCAAAGATGGTGCTACTCTCATCAACTGCCCAGAAAATGTTAGTAATTCCTGCCCTGAATACTTCATGTCTCAAGAACTCCATACTCTGACTTCCTCTCACGAGATCATCGCATTGCAAGACCGAATGAACCAAAACTGGCCAGACCTAAAGGAAGGGAACTCATTGGAAGCGTGGAGGAGCAAATGGGCAATCCACGGGACATGCTCGGCGGCAGTGTTCAATCAGTTCGAATACTTTAGCTTGGGTTTGGACACCTACGGCCGCTATGCCATGTTGTCAATTCTAGACAAAGGAGGTTTGGCTCCTAGCTCTTATAAGCATATAGGCAAGACCGAGTTTATCGACGTCATGGCCGAGTTCACGTCGAAGAAGGGCGGCGTCATTTGTGCCTCCGACCACCGTGGCCGAAGACAGGTCCAGAAGGTCATTTTATGTTATGCCAAAGATGGTGCTACTCTCATCAACTGCCCAGAGAATGTTAGTAATTCCTGCCCTGAATACTTCATGTGGCTTGGCTTGGATGATTGA

Protein sequence

MTKKASVFLVSIWLLIALSKQADFDYVQMVLEWQPAMCNLGKSCKIEPKNKFSIHGVWPSLYSRGQIGPCYGPPFNPDKIIALQDRMNQNWPDLKEGNSLEAWRSKWAIHGTCSAAVFNQFEYFSLGLDTYGRYAMLSILDKGGVAPSSYRYIAKTQFIDVITEFTSKKGGVICATDHRGRRQVQKVILCYAKDGATLINCPENVSNSCPEYFMSQELHTLTSSHEIIALQDRMNQNWPDLKEGNSLEAWRSKWAIHGTCSAAVFNQFEYFSLGLDTYGRYAMLSILDKGGLAPSSYKHIGKTEFIDVMAEFTSKKGGVICASDHRGRRQVQKVILCYAKDGATLINCPENVSNSCPEYFMWLGLDD
Homology
BLAST of Spg015195 vs. NCBI nr
Match: XP_038899255.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 335.5 bits (859), Expect = 5.8e-88
Identity = 150/214 (70.09%), Postives = 181/214 (84.58%), Query Frame = 0

Query: 1   MTKKASVFLVSIWLLIALSKQADFDYVQMVLEWQPAMCNLGKSCKIEPKNKFSIHGVWPS 60
           M KKASVFLVS++L IALSKQ  FDYVQ+V EWQPA+CNLGKSCKI+P+N+F IHGVWPS
Sbjct: 2   MRKKASVFLVSLFLSIALSKQDAFDYVQVVYEWQPAICNLGKSCKIQPENEFGIHGVWPS 61

Query: 61  LYSRGQIGPCYGPPFNPDKIIALQDRMNQNWPDLKEGNSLEAWRSKWAIHGTCSAAVFNQ 120
           +YS+GQIGPC GP F+P KI++LQDRM+Q W DL+ GN +EAW SKWAIHGTCSA VF++
Sbjct: 62  VYSKGQIGPCSGPSFDPLKIVSLQDRMSQYWQDLENGNPIEAWASKWAIHGTCSATVFDE 121

Query: 121 FEYFSLGLDTYGRYAMLSILDKGGVAPSSYRYIAKTQFIDVITEFTSKKGGVICATDHRG 180
           F+YF LGLDTYGR+AMLS L  GG+ PSS +Y+ KT F+  I + TSKKGGVICA D RG
Sbjct: 122 FKYFCLGLDTYGRHAMLSFLVHGGLVPSSSKYLDKTSFVKAIADMTSKKGGVICAVDQRG 181

Query: 181 RRQVQKVILCYAKDGATLINCPENVSNSCPEYFM 215
           R Q+QK +LCYAKDG TLI+CP+NVS+SCP+ F+
Sbjct: 182 RTQLQKAVLCYAKDGDTLIDCPDNVSSSCPDNFL 215

BLAST of Spg015195 vs. NCBI nr
Match: XP_004149779.2 (ribonuclease MC [Cucumis sativus])

HSP 1 Score: 321.6 bits (823), Expect = 8.6e-84
Identity = 147/214 (68.69%), Postives = 174/214 (81.31%), Query Frame = 0

Query: 1   MTKKASVFLVSIWLLIALSKQADFDYVQMVLEWQPAMCNLGKSCKIEPKNKFSIHGVWPS 60
           M KKASVFLVS+ LLI LSKQ  FDY Q+V EWQPA+CNLG SCKI+PKN+F IHGVWPS
Sbjct: 2   MGKKASVFLVSLLLLIGLSKQDAFDYFQVVHEWQPAICNLGNSCKIQPKNEFGIHGVWPS 61

Query: 61  LYSRGQIGPCYGPPFNPDKIIALQDRMNQNWPDLKEGNSLEAWRSKWAIHGTCSAAVFNQ 120
           +YS+GQIGPC GP F+P KII+LQDRMNQ W DL+ GNS+EAW SKW +HGTCSAA F+Q
Sbjct: 62  VYSKGQIGPCCGPSFDPLKIISLQDRMNQYWKDLENGNSIEAWASKWEMHGTCSAAGFDQ 121

Query: 121 FEYFSLGLDTYGRYAMLSILDKGGVAPSSYRYIAKTQFIDVITEFTSKKGGVICATDHRG 180
           F+YF LGLDTYGR+A+ S LD+ G+APSS +Y+AK  FI  I   T KKGGVICA D   
Sbjct: 122 FKYFCLGLDTYGRHAIFSFLDREGLAPSSSKYVAKASFITAIANSTLKKGGVICAVDQYR 181

Query: 181 RRQVQKVILCYAKDGATLINCPENVSNSCPEYFM 215
           R Q+QK +LCY KDG TLI+CP+NVS+SCP+ F+
Sbjct: 182 RIQLQKAVLCYVKDGHTLIDCPDNVSSSCPDNFV 215

BLAST of Spg015195 vs. NCBI nr
Match: KGN45018.2 (hypothetical protein Csa_016393 [Cucumis sativus])

HSP 1 Score: 321.6 bits (823), Expect = 8.6e-84
Identity = 147/214 (68.69%), Postives = 174/214 (81.31%), Query Frame = 0

Query: 1   MTKKASVFLVSIWLLIALSKQADFDYVQMVLEWQPAMCNLGKSCKIEPKNKFSIHGVWPS 60
           M KKASVFLVS+ LLI LSKQ  FDY Q+V EWQPA+CNLG SCKI+PKN+F IHGVWPS
Sbjct: 1   MGKKASVFLVSLLLLIGLSKQDAFDYFQVVHEWQPAICNLGNSCKIQPKNEFGIHGVWPS 60

Query: 61  LYSRGQIGPCYGPPFNPDKIIALQDRMNQNWPDLKEGNSLEAWRSKWAIHGTCSAAVFNQ 120
           +YS+GQIGPC GP F+P KII+LQDRMNQ W DL+ GNS+EAW SKW +HGTCSAA F+Q
Sbjct: 61  VYSKGQIGPCCGPSFDPLKIISLQDRMNQYWKDLENGNSIEAWASKWEMHGTCSAAGFDQ 120

Query: 121 FEYFSLGLDTYGRYAMLSILDKGGVAPSSYRYIAKTQFIDVITEFTSKKGGVICATDHRG 180
           F+YF LGLDTYGR+A+ S LD+ G+APSS +Y+AK  FI  I   T KKGGVICA D   
Sbjct: 121 FKYFCLGLDTYGRHAIFSFLDREGLAPSSSKYVAKASFITAIANSTLKKGGVICAVDQYR 180

Query: 181 RRQVQKVILCYAKDGATLINCPENVSNSCPEYFM 215
           R Q+QK +LCY KDG TLI+CP+NVS+SCP+ F+
Sbjct: 181 RIQLQKAVLCYVKDGHTLIDCPDNVSSSCPDNFV 214

BLAST of Spg015195 vs. NCBI nr
Match: XP_022143591.1 (ribonuclease 1-like [Momordica charantia])

HSP 1 Score: 189.5 bits (480), Expect = 5.1e-44
Identity = 82/134 (61.19%), Postives = 107/134 (79.85%), Query Frame = 0

Query: 234 MNQNWPDLKEGNSLEAWRSKWAIHGTCSAAVFNQFEYFSLGLDTYGRYAMLSILDKGGLA 293
           M + W DL  G++  AW++KWAIHGTCSA VF++F+YF +GLDTYGR+A+LS L  GGL 
Sbjct: 1   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV 60

Query: 294 PSSYKHIGKTEFIDVMAEFTSKKGGVICASDHRGRRQVQKVILCYAKDGATLINCPENVS 353
           PSS +   KT FI  +A+ TSKKGGVICA+D RGR Q++ V+LCY+KDGAT+I+CP+NVS
Sbjct: 61  PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS 120

Query: 354 NSCPEYFMWLGLDD 368
           +SCP+ F+WL L D
Sbjct: 121 SSCPDKFLWLALGD 134

BLAST of Spg015195 vs. NCBI nr
Match: XP_022159333.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 175.6 bits (444), Expect = 7.7e-40
Identity = 86/218 (39.45%), Postives = 123/218 (56.42%), Query Frame = 0

Query: 1   MTKKASVFLVSIWLLIALSKQADFDYVQMVLEWQPAMCN---LGKSCKIEPKNKFSIHGV 60
           M K+  +F++ + L IA S+QA FDY Q+V +WQPA CN   L   C   P N+FSIHGV
Sbjct: 1   MAKREIIFVLVLLLFIASSEQASFDYYQVVFQWQPATCNDITLPTRCVQNPDNRFSIHGV 60

Query: 61  WPSLYSRGQIGPCYGPPFNPDKIIALQDRMNQNWPDLKEGNSLEAWRSKWAIHGTCSAAV 120
           WPS  S  ++GPC G  F+P  I++++  +NQNWPD+    +   W  +W  HGTCS  +
Sbjct: 61  WPSKTSGSRVGPCNGTAFDPATIVSIRAGLNQNWPDVIRRKNQVFWSDEWRKHGTCSEDL 120

Query: 121 FNQFEYFSLGLDTYGRYA--MLSILDKGGVAPSSYRYIAKTQFIDVITEFTSKKGGVICA 180
            NQ +YF+ GL+ Y      ++  L K  + P+  +Y  K      I+    K+ G  C 
Sbjct: 121 LNQLQYFTFGLNIYHDEGKDIVEKLGKKKIIPNGKKY-KKADVEKAISSVIGKRLGFRCN 180

Query: 181 TDHRGRRQVQKVILCYAKDGATLINCPENVSNSCPEYF 214
            D     Q+ ++  CYAKD +TL +CP N +N CP  F
Sbjct: 181 IDQSNYHQLHEISFCYAKDASTLQDCPSNATN-CPTKF 216

BLAST of Spg015195 vs. ExPASy Swiss-Prot
Match: Q7M438 (Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3)

HSP 1 Score: 104.4 bits (259), Expect = 2.8e-21
Identity = 53/193 (27.46%), Postives = 94/193 (48.70%), Query Frame = 0

Query: 19  SKQADFDYVQMVLEWQPAMCNLGKSCKIEPKNKFSIHGVWPSLYSRGQIGPCYGPPFNPD 78
           SK  DFD+   V +W  + C+     + + +  F+IHG+WP          C GP FN +
Sbjct: 27  SKPGDFDFYLFVQQWIYSYCDSQTCIQNKEREAFTIHGLWPENSDGSYPSFCSGPSFNVN 86

Query: 79  KIIALQDRMNQNWPDLKEGNSLEAWRSKWAIHGTCS--AAVFNQFEYFSLGLDTYGRYAM 138
            I  L+D++N +WP L  G + + W  +++ HGTCS    + +  +YF+ G+  Y  + +
Sbjct: 87  AIQDLEDQLNFDWPSL-TGPNTDFWTHEFSKHGTCSITGPITDIHDYFATGIKLYTEFNI 146

Query: 139 LSILDKGGVAPSSYRYIAKTQFIDVITEFTSKKGGVICATDHRGRRQVQKVILCYAKDGA 198
            + L+   + PS           + IT     K G+ C++      Q+  V +C  K+  
Sbjct: 147 TAALESENIYPSDSNTYKPVDITNAITTHFGGKPGIQCSSG-----QLSTVAVCIDKNSL 206

Query: 199 TLINCPENVSNSC 210
           ++++CP+    SC
Sbjct: 207 SIMDCPDLQGWSC 213

BLAST of Spg015195 vs. ExPASy Swiss-Prot
Match: Q38717 (Ribonuclease S-4 OS=Antirrhinum hispanicum OX=49039 GN=S4 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.2e-19
Identity = 51/189 (26.98%), Postives = 94/189 (49.74%), Query Frame = 0

Query: 22  ADFDYVQMVLEWQPAMCNL-GKSCKIEP-KNKFSIHGVWPSLYSRGQIGPCYGPPFNPDK 81
           A  DY+++VL+W  + C +  + C+  P  + F+IHG+WP  Y+R     C    F    
Sbjct: 31  AKCDYLKLVLQWPKSFCLINSRKCQRNPLPSNFTIHGLWPDNYTRQAPQSCTTNNFQRFT 90

Query: 82  IIALQDRMNQNWPDLKE----GNSLEAWRSKWAIHGTCSAAVFNQFEYFSLGLDTYGRYA 141
              ++ RM ++WPDLK+    G S   W+ +W  HG+C         YF   L+   R  
Sbjct: 91  DTDIEQRMEESWPDLKQQSIAGLSYNFWQDQWRKHGSCCFPPHESEIYFLKALELKDRLD 150

Query: 142 MLSILDKGGVAPSSYRYIAKTQFIDVITEFTSKKGGVICATDHRGRRQVQKVILCYAKDG 201
           +L+IL+     P + +  +  +  + I+    K   + CA  +     +++V++C   +G
Sbjct: 151 VLTILENNNFNPGTPQPFSVLRVFNTISRAIGKTPILKCAQSY-----LKEVVICVDNNG 210

Query: 202 ATLINCPEN 205
           A++++CP +
Sbjct: 211 ASVVHCPRS 214

BLAST of Spg015195 vs. ExPASy Swiss-Prot
Match: Q7SID5 (Ribonuclease S-F11 OS=Nicotiana alata OX=4087 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 6.8e-15
Identity = 48/193 (24.87%), Postives = 91/193 (47.15%), Query Frame = 0

Query: 23  DFDYVQMVLEWQPAMCNLGKSCKIEPKNKFSIHGVWPSLYSRGQIGPCYGPPFNPDKIIA 82
           DF+Y+Q+VL W  + C      +I P N F+IHG+WP    + ++  C   P        
Sbjct: 1   DFEYLQLVLTWPASFCYANHCERIAP-NNFTIHGLWPD-NVKTRLHNCKPKPTYSYFTGK 60

Query: 83  LQDRMNQNWPDLK-----EGNSLEAWRSKWAIHGTCSAAVFNQFEYFSLGLDTYGRYAML 142
           + + ++++W  LK           +W+ ++  HG+C    +NQ  YF L L    ++ +L
Sbjct: 61  MLNDLDKHWMQLKFEQDYGRTEQPSWKYQYIKHGSCCQKRYNQNTYFGLALRLKDKFDLL 120

Query: 143 SILDKGGVAP-SSYRYIAKTQFIDVITEFTSKKGGVICATDHRGRRQVQKVILCYAKDGA 202
             L    + P SSY +       D I   + +   + CA   +G  ++ ++ +C+  +  
Sbjct: 121 RTLQTHRIIPGSSYTF---QDIFDAIKTVSQENPDIKCAEVTKGTPELYEIGICFTPNAD 180

Query: 203 TLINCPENVSNSC 210
           ++  CP+  S++C
Sbjct: 181 SMFRCPQ--SDTC 186

BLAST of Spg015195 vs. ExPASy Swiss-Prot
Match: Q7M456 (Ribonuclease Oy OS=Crassostrea gigas OX=29159 PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 2.9e-13
Identity = 43/134 (32.09%), Postives = 70/134 (52.24%), Query Frame = 0

Query: 23  DFDYVQMVLEWQPAMCNLGKSCKI-EPKNKFSIHGVWPSLYSRGQIGP--CYGP-PFNPD 82
           D++Y     +W  A+C   KSC I +    + IHG+WPS  +  + GP  C G  PF+ +
Sbjct: 2   DWNYFTFAQQWPIAVCAEHKSCFIPDSVVGWGIHGLWPSSDTESK-GPENCNGSWPFDIN 61

Query: 83  KIIALQDRMNQNWPDLKEGNSLEA-WRSKWAIHGTCSA---AVFNQFEYFSLGLDTYGRY 142
            ++ L   + + WP+L       + W  +W+ HGTC+    A  N+ +YF +GL  + +Y
Sbjct: 62  NVMPLVPELKKYWPNLYPDTKANSFWEHEWSKHGTCATSLPATSNELKYFGMGLKLHAKY 121

Query: 143 AMLSILDKGGVAPS 149
            +  IL   G+ PS
Sbjct: 122 NISRILVNQGILPS 134

BLAST of Spg015195 vs. ExPASy Swiss-Prot
Match: Q40379 (Ribonuclease S-6 OS=Nicotiana alata OX=4087 PE=1 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 6.4e-13
Identity = 55/207 (26.57%), Postives = 99/207 (47.83%), Query Frame = 0

Query: 6   SVFLVSIWLLIALSKQADFDYVQMVLEWQPAMCNLGKSCKIEPKNKFSIHGVWPSLYSRG 65
           SVF++ ++ L  +     F+Y+Q+VL+W  A C+    CK  P N F+IHG+WP   S  
Sbjct: 8   SVFVIFLFALSPI--YGAFEYMQLVLQWPTAFCHT-TPCKNIPSN-FTIHGLWPDNVST- 67

Query: 66  QIGPCYGPPFNPDKIIALQDRMNQN-----WPDL--KEGNSLEA---WRSKWAIHGTCSA 125
            +  C       D    + D   +N     WPDL  ++ + ++    WR ++  HGTC +
Sbjct: 68  TLNFC----GKEDDYNIIMDGPEKNGLYVRWPDLIREKADCMKTQNFWRREYIKHGTCCS 127

Query: 126 AVFNQFEYFSLGLDTYGRYAMLSILDKGGVAPSSYRYIAKTQFIDVITEFTSKKGGVICA 185
            ++NQ +YF L +    ++ +L+ L   G+    Y+Y  + +  + I   T     + C 
Sbjct: 128 EIYNQVQYFRLAMALKDKFDLLTSLKNHGII-RGYKYTVQ-KINNTIKTVTKGYPNLSCT 187

Query: 186 TDHRGRRQVQKVILCYAKDGATLINCP 203
                 +++  V +C+      +I+CP
Sbjct: 188 KG----QELWFVGICFDSTAKNVIDCP 199

BLAST of Spg015195 vs. ExPASy TrEMBL
Match: A0A0A0K4L1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G351900 PE=3 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 1.3e-45
Identity = 86/134 (64.18%), Postives = 104/134 (77.61%), Query Frame = 0

Query: 234 MNQNWPDLKEGNSLEAWRSKWAIHGTCSAAVFNQFEYFSLGLDTYGRYAMLSILDKGGLA 293
           MNQ W DL+ GNS+EAW SKW +HGTCSAA F+QF+YF LGLDTYGR+A+ S LD+ GLA
Sbjct: 1   MNQYWKDLENGNSIEAWASKWEMHGTCSAAGFDQFKYFCLGLDTYGRHAIFSFLDREGLA 60

Query: 294 PSSYKHIGKTEFIDVMAEFTSKKGGVICASDHRGRRQVQKVILCYAKDGATLINCPENVS 353
           PSS K++ K  FI  +A  T KKGGVICA D   R Q+QK +LCY KDG TLI+CP+NVS
Sbjct: 61  PSSSKYVAKASFITAIANSTLKKGGVICAVDQYRRIQLQKAVLCYVKDGHTLIDCPDNVS 120

Query: 354 NSCPEYFMWLGLDD 368
           +SCP+ F+WL L D
Sbjct: 121 SSCPDNFVWLALGD 134

BLAST of Spg015195 vs. ExPASy TrEMBL
Match: A0A6J1CQQ9 (ribonuclease 1-like OS=Momordica charantia OX=3673 GN=LOC111013449 PE=3 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 2.5e-44
Identity = 82/134 (61.19%), Postives = 107/134 (79.85%), Query Frame = 0

Query: 234 MNQNWPDLKEGNSLEAWRSKWAIHGTCSAAVFNQFEYFSLGLDTYGRYAMLSILDKGGLA 293
           M + W DL  G++  AW++KWAIHGTCSA VF++F+YF +GLDTYGR+A+LS L  GGL 
Sbjct: 1   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV 60

Query: 294 PSSYKHIGKTEFIDVMAEFTSKKGGVICASDHRGRRQVQKVILCYAKDGATLINCPENVS 353
           PSS +   KT FI  +A+ TSKKGGVICA+D RGR Q++ V+LCY+KDGAT+I+CP+NVS
Sbjct: 61  PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS 120

Query: 354 NSCPEYFMWLGLDD 368
           +SCP+ F+WL L D
Sbjct: 121 SSCPDKFLWLALGD 134

BLAST of Spg015195 vs. ExPASy TrEMBL
Match: A0A6J1DYI5 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025745 PE=3 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 3.7e-40
Identity = 86/218 (39.45%), Postives = 123/218 (56.42%), Query Frame = 0

Query: 1   MTKKASVFLVSIWLLIALSKQADFDYVQMVLEWQPAMCN---LGKSCKIEPKNKFSIHGV 60
           M K+  +F++ + L IA S+QA FDY Q+V +WQPA CN   L   C   P N+FSIHGV
Sbjct: 1   MAKREIIFVLVLLLFIASSEQASFDYYQVVFQWQPATCNDITLPTRCVQNPDNRFSIHGV 60

Query: 61  WPSLYSRGQIGPCYGPPFNPDKIIALQDRMNQNWPDLKEGNSLEAWRSKWAIHGTCSAAV 120
           WPS  S  ++GPC G  F+P  I++++  +NQNWPD+    +   W  +W  HGTCS  +
Sbjct: 61  WPSKTSGSRVGPCNGTAFDPATIVSIRAGLNQNWPDVIRRKNQVFWSDEWRKHGTCSEDL 120

Query: 121 FNQFEYFSLGLDTYGRYA--MLSILDKGGVAPSSYRYIAKTQFIDVITEFTSKKGGVICA 180
            NQ +YF+ GL+ Y      ++  L K  + P+  +Y  K      I+    K+ G  C 
Sbjct: 121 LNQLQYFTFGLNIYHDEGKDIVEKLGKKKIIPNGKKY-KKADVEKAISSVIGKRLGFRCN 180

Query: 181 TDHRGRRQVQKVILCYAKDGATLINCPENVSNSCPEYF 214
            D     Q+ ++  CYAKD +TL +CP N +N CP  F
Sbjct: 181 IDQSNYHQLHEISFCYAKDASTLQDCPSNATN-CPTKF 216

BLAST of Spg015195 vs. ExPASy TrEMBL
Match: A0A6J1E0X1 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 1.7e-37
Identity = 87/220 (39.55%), Postives = 137/220 (62.27%), Query Frame = 0

Query: 1   MTKKASVFLVSIWLLIALSKQA-DFDYVQMVLEWQPAMCN---LGKSCKIEPKNKFSIHG 60
           M K+  + ++S++L I+ S+   +FDY Q+VL+WQPA C+   L   C   P++++S+HG
Sbjct: 1   MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHG 60

Query: 61  VWPSLYSRGQIGPCYG-PPFNPDKIIALQDRMNQNWPDLKEGNSLEAWRSKWAIHGTCSA 120
           VWPSL S G +  C G PP++   I+ +Q R++Q+WP++  G + + W  +W  HGTCS+
Sbjct: 61  VWPSL-SSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSS 120

Query: 121 AVFNQFEYFSLGLDTYGRYAMLSILDKGGVAPSSYRYIAKTQFIDVITEFTSKKGGVICA 180
           A  NQF YF+ GL  Y  Y + SIL + G+ P+  +Y  KT   + I   T K   + C 
Sbjct: 121 AYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQY-TKTVVQNAIYSATGKWPALRCN 180

Query: 181 TDHR-GRRQVQKVILCYAKDGATLINCPENVSNSCPEYFM 215
            ++R G  Q+ ++ LC++K+G  LI+CP NV  +C EYF+
Sbjct: 181 NNYRTGNMQLHEISLCFSKNGQILIDCPFNVV-TCNEYFV 217

BLAST of Spg015195 vs. ExPASy TrEMBL
Match: A0A6J1E266 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 1.5e-36
Identity = 86/217 (39.63%), Postives = 126/217 (58.06%), Query Frame = 0

Query: 1   MTKKASVFLVSIWLLIALSKQADFDYVQMVLEWQPAMC---NLGKSCKIEPKNKFSIHGV 60
           M KK  V + S  ++IA     D++Y Q+ L+WQPA C   NL   C   P+++FSIHGV
Sbjct: 1   MEKKGIVLVFSTLVVIA---SCDYNYYQVTLQWQPATCSDANLQTPCVNTPQDRFSIHGV 60

Query: 61  WPSLYSRGQIGPCYG-PPFNPDKIIALQDRMNQNWPDLKEGNSLEAWRSKWAIHGTCSAA 120
           WP+L S G    C G P ++  KI  +++R+N +WP +  G +   W  +W  HGTCS  
Sbjct: 61  WPTL-SSGSYPQCPGTPAYDGSKISTIRNRLNADWPSVIYGTNPTLWSHEWTTHGTCSYP 120

Query: 121 VFNQFEYFSLGLDTYGRYAMLSILDKGGVAPSSYRYIAKTQFIDVITEFTSKKGGVICAT 180
             +Q +YF+ GLDTY +Y + S LDK  + PS   Y +K+   + I + T K  G+ C T
Sbjct: 121 SLSQLDYFTFGLDTYAKYDIYSALDKEDIYPSEDVY-SKSSINNAIRKITQKLPGLRCNT 180

Query: 181 -DHRGRRQVQKVILCYAKDGATLINCPENVSNSCPEY 213
            D+   RQ+ ++ILC+AK    +I+CP NV  +C  Y
Sbjct: 181 NDYSRERQLHEIILCFAKSNRAIIDCPSNVV-TCGNY 211

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038899255.15.8e-8870.09ribonuclease MC-like [Benincasa hispida][more]
XP_004149779.28.6e-8468.69ribonuclease MC [Cucumis sativus][more]
KGN45018.28.6e-8468.69hypothetical protein Csa_016393 [Cucumis sativus][more]
XP_022143591.15.1e-4461.19ribonuclease 1-like [Momordica charantia][more]
XP_022159333.17.7e-4039.45ribonuclease MC-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q7M4382.8e-2127.46Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3[more]
Q387171.2e-1926.98Ribonuclease S-4 OS=Antirrhinum hispanicum OX=49039 GN=S4 PE=2 SV=1[more]
Q7SID56.8e-1524.87Ribonuclease S-F11 OS=Nicotiana alata OX=4087 PE=1 SV=1[more]
Q7M4562.9e-1332.09Ribonuclease Oy OS=Crassostrea gigas OX=29159 PE=1 SV=1[more]
Q403796.4e-1326.57Ribonuclease S-6 OS=Nicotiana alata OX=4087 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0K4L11.3e-4564.18Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G351900 PE=3 SV=1[more]
A0A6J1CQQ92.5e-4461.19ribonuclease 1-like OS=Momordica charantia OX=3673 GN=LOC111013449 PE=3 SV=1[more]
A0A6J1DYI53.7e-4039.45ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025745 PE=3 SV=1[more]
A0A6J1E0X11.7e-3739.55ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1[more]
A0A6J1E2661.5e-3639.63ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036430Ribonuclease T2-like superfamilyGENE3D3.90.730.10coord: 217..363
e-value: 3.9E-27
score: 97.6
coord: 18..216
e-value: 1.6E-44
score: 154.4
IPR036430Ribonuclease T2-like superfamilySUPERFAMILY55895Ribonuclease Rh-likecoord: 229..362
IPR036430Ribonuclease T2-like superfamilySUPERFAMILY55895Ribonuclease Rh-likecoord: 23..214
IPR001568Ribonuclease T2-likePFAMPF00445Ribonuclease_T2coord: 224..348
e-value: 6.7E-18
score: 65.5
coord: 24..201
e-value: 1.5E-29
score: 103.4
IPR001568Ribonuclease T2-likePANTHERPTHR11240RIBONUCLEASE T2coord: 221..358
coord: 20..212
NoneNo IPR availablePANTHERPTHR11240:SF51RIBONUCLEASE 2coord: 221..358
NoneNo IPR availablePANTHERPTHR11240:SF51RIBONUCLEASE 2coord: 20..212
NoneNo IPR availableCDDcd00374RNase_T2coord: 24..210
e-value: 4.35352E-43
score: 146.447
NoneNo IPR availableCDDcd00374RNase_T2coord: 223..357
e-value: 3.75882E-24
score: 96.3711

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg015195.1Spg015195.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0033897 ribonuclease T2 activity
molecular_function GO:0003723 RNA binding