Spg014631 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg014631
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionrRNA N-glycosidase
Locationscaffold3: 36741263 .. 36743418 (-)
RNA-Seq ExpressionSpg014631
SyntenySpg014631
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGGGGCCGTTAAGGGCCGAATCCGGATTCCGAATCCTGGGCATGATCTGTAACACCCCAGGCCCAGGATTTGGAATCCGGATTCGGCATCCTGGGCCTGGGGTGTTACAGATGGTATCAGAGCGGAACCTCTCCTAGTAGGATGTGGTTCGGGGACGAACCAAGGCGGAAGCTGGTGGGCATGTGACACCCGAAGTTAGGAGGGGTACATGAATGAACCGAGATCACATCCGAATGAGAGGGATCCTGAGGACATGAAAGTATGGCCTAGAGAAGCTTAAAAGAATTGATAGATACTATGTAACACCCCAGGCCCAGGATTCGGAATCCGGATTCGGCCCTTAACGGCCCCGGCATTCCCCTGCGACCTGGCCACGTTACCATACTTATCTGAAACTGCTCCTAAGAGTGAAGACTGTCCCCACAAACCAACACGGGGTCCTTTTCGGATGCTTTGTCCTCACTCACATGCTTCCTAGGAAAATTCCCAGGAGGTCACCCAACATAGAACTTCTCCAAGCTAAGAACACTTAACTTCAAAGTTCCTATGATTGAGCCACCGAAAAGGAAGGTGCACCTTGTTGGTATAGGTAGTATCTATCAATTCTTTTAAGCTTCTCTAGGCCATACTTTCATGTCCTCAGGATCCCTCTCATTCGGATGTGATCTCGGTTCATTCATGTACCCCTCCTAACTTCGGGTGTCACACAAGGAGCTGACGAGGACATCCGGGCAGAAATAGGACTAGGAGACCGACCCAGAGGAAGAACCGACCAAAGGACCGGGCCAACTTGGCTCGACCCATATGGTCGGCCTTGGCCCAGGCCGAGGCCGAGCTCGGTCGCCTCCTTTCGGTCCCTGATGCCTCTGGCCTCCCCGGTTTCGTCTAGTTCGTCCCGAAACGCCTCCGAATTCCTAAAAACCCTAGGAGCATGAGCAGGTATTTATACCCCTCTTCGCCATTGAAGAGAGAATCTGAACTCTAAACCCGAACTCTATTCTCTATTTTCTCCTCTTGCTCTTATTTCCTTGCTTCCCATCGTTCTGCTTGCTGACTTAAGCATCGGAGACGGTGTGGCTAGCACCACACCGGTGTGCAGGTTTACTGTGTTGCAGGCCACGTCTTCCCCCCTCTCAAACAAATTTACCGTTGGTGGCACGTGAAGGTCAGGTGAGTTCCTCTGACCAAATTTGACCATCAACAATTGTCATCATTTTAATGGTGGTGTGTAGGAGATCCCTACATACCGGTATGCAGCTAAGTATTGTCATCGTCACAAAATTGCTTCGATAGATGTGGTAGCTGTGTATAAAACCACACGTTGAGATGCTCCAAAGGCATCCAAAAATATCGAGTGGGAAAGATGAAGAGATTTACAGTGCTAATTCTCGCCATCTTCCTTGCAGCTTCAACTGTTGAAGCCGATGTGAGCTTCAGTTTGTCAGGTTCTTCCTCCACATCTTATAGCAAGTTCATCGGAGATCTGAGGAAGGCTCTTCCATCTAGTGGGACAGTCTACAACATAACTCTCTTACTCTCCTCTGCCTCGGGCGCAAGCCGCTACACTCTCATGAAACTCTCCAATTACGACGGCAAAGCCATCACGGTTGCCATAGATGTAACCAACGTTTACATCATGGGCTATCTCGTCAATTCAACATCCTACTTTTTCAACGAGTCTGATGCTAAATTAGCTTCTCAATACGTATTCAAAGGCAGTACCATCGTCACGCTTCCATATTCTGGCAATTACGAAAAGCTTCAAACTGCTGAAGGGAAGATAAGAGAGAAGATTCCACTGGGATTCCCAGCTTTGGACAGTGCGATTACCACCTTGTTTCATTACGACTCCACGGCTGCTGCTGCGGCATTCCTCGTAATCATTCAGACCACTGCTGAGGCTTCCAGATTCAAGTATATCGAGGGACAGATTATTGAGAGAATTTCGAAAAACGGGGTGCCGAGTCTGGCGACTATAAGTTTAGAAAACGAATGGTCTGCTCTCTCTAAACAAATTCAGTTGGAACAGACGAATAACGGAACGTTTAAAACTCCCGTTGTGATAACGGACGATAAAGGCCAACGAGTTGAAATAACCAACGTCACTTCGAAAGTTGTAACCAAGAACATCAAATTGCTTCTAAACAAGTAA

mRNA sequence

ATGCCGGGGCCGTTAAGGGCCGAATCCGGATTCCGAATCCTGGGCATGATCTGTAACACCCCAGGCCCAGGATTTGGAATCCGGATTCGGCATCCTGGGCCTGGGGTGTTACAGATGTTCGTCCCGAAACGCCTCCGAATTCCTAAAAACCCTAGGAGCATGAGCAGCATCGGAGACGGTGTGGCTAGCACCACACCGGTGTGCAGGAGATCCCTACATACCGCTTCAACTGTTGAAGCCGATGTGAGCTTCAGTTTGTCAGGTTCTTCCTCCACATCTTATAGCAAGTTCATCGGAGATCTGAGGAAGGCTCTTCCATCTAGTGGGACAGTCTACAACATAACTCTCTTACTCTCCTCTGCCTCGGGCGCAAGCCGCTACACTCTCATGAAACTCTCCAATTACGACGGCAAAGCCATCACGGTTGCCATAGATGTAACCAACGTTTACATCATGGGCTATCTCGTCAATTCAACATCCTACTTTTTCAACGAGTCTGATGCTAAATTAGCTTCTCAATACGTATTCAAAGGCAGTACCATCGTCACGCTTCCATATTCTGGCAATTACGAAAAGCTTCAAACTGCTGAAGGGAAGATAAGAGAGAAGATTCCACTGGGATTCCCAGCTTTGGACAGTGCGATTACCACCTTGTTTCATTACGACTCCACGGCTGCTGCTGCGGCATTCCTCGTAATCATTCAGACCACTGCTGAGGCTTCCAGATTCAAGTATATCGAGGGACAGATTATTGAGAGAATTTCGAAAAACGGGGTGCCGAGTCTGGCGACTATAAGTTTAGAAAACGAATGGTCTGCTCTCTCTAAACAAATTCAGTTGGAACAGACGAATAACGGAACGTTTAAAACTCCCGTTGTGATAACGGACGATAAAGGCCAACGAGTTGAAATAACCAACGTCACTTCGAAAGTTGTAACCAAGAACATCAAATTGCTTCTAAACAAGTAA

Coding sequence (CDS)

ATGCCGGGGCCGTTAAGGGCCGAATCCGGATTCCGAATCCTGGGCATGATCTGTAACACCCCAGGCCCAGGATTTGGAATCCGGATTCGGCATCCTGGGCCTGGGGTGTTACAGATGTTCGTCCCGAAACGCCTCCGAATTCCTAAAAACCCTAGGAGCATGAGCAGCATCGGAGACGGTGTGGCTAGCACCACACCGGTGTGCAGGAGATCCCTACATACCGCTTCAACTGTTGAAGCCGATGTGAGCTTCAGTTTGTCAGGTTCTTCCTCCACATCTTATAGCAAGTTCATCGGAGATCTGAGGAAGGCTCTTCCATCTAGTGGGACAGTCTACAACATAACTCTCTTACTCTCCTCTGCCTCGGGCGCAAGCCGCTACACTCTCATGAAACTCTCCAATTACGACGGCAAAGCCATCACGGTTGCCATAGATGTAACCAACGTTTACATCATGGGCTATCTCGTCAATTCAACATCCTACTTTTTCAACGAGTCTGATGCTAAATTAGCTTCTCAATACGTATTCAAAGGCAGTACCATCGTCACGCTTCCATATTCTGGCAATTACGAAAAGCTTCAAACTGCTGAAGGGAAGATAAGAGAGAAGATTCCACTGGGATTCCCAGCTTTGGACAGTGCGATTACCACCTTGTTTCATTACGACTCCACGGCTGCTGCTGCGGCATTCCTCGTAATCATTCAGACCACTGCTGAGGCTTCCAGATTCAAGTATATCGAGGGACAGATTATTGAGAGAATTTCGAAAAACGGGGTGCCGAGTCTGGCGACTATAAGTTTAGAAAACGAATGGTCTGCTCTCTCTAAACAAATTCAGTTGGAACAGACGAATAACGGAACGTTTAAAACTCCCGTTGTGATAACGGACGATAAAGGCCAACGAGTTGAAATAACCAACGTCACTTCGAAAGTTGTAACCAAGAACATCAAATTGCTTCTAAACAAGTAA

Protein sequence

MPGPLRAESGFRILGMICNTPGPGFGIRIRHPGPGVLQMFVPKRLRIPKNPRSMSSIGDGVASTTPVCRRSLHTASTVEADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTKNIKLLLNK
Homology
BLAST of Spg014631 vs. NCBI nr
Match: Q00465.1 (RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Luffa aegyptiaca] >CAA44229.1 a-luffin [Luffa aegyptiaca])

HSP 1 Score: 446.4 bits (1147), Expect = 2.0e-121
Identity = 239/247 (96.76%), Postives = 242/247 (97.98%), Query Frame = 0

Query: 75  ASTVEADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLSN 134
           ASTVEADV FSLSGSSSTSYSKFIGDLRKALPS+GTVYNITLLLSSASGASRYTLM LSN
Sbjct: 15  ASTVEADVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLSSASGASRYTLMTLSN 74

Query: 135 YDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQ 194
           YDGKAITVA+DVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQ
Sbjct: 75  YDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQ 134

Query: 195 TAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERI 254
           TA GKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERI
Sbjct: 135 TAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERI 194

Query: 255 SKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTK 314
           SKN VPSLATISLENEWSALSKQIQL QTNNGTFKTPVVITDDKGQRVEITNVTSKVVTK
Sbjct: 195 SKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTK 254

Query: 315 NIKLLLN 322
           NI+LLLN
Sbjct: 255 NIQLLLN 261

BLAST of Spg014631 vs. NCBI nr
Match: P84530.2 (RecName: Full=Ribosome-inactivating protein luffaculin 1; AltName: Full=rRNA N-glycosidase [Luffa acutangula] >2OQA_A X-ray Sequence and Crystal Structure of Luffaculin 1, a Novel Type 1 Ribosome-inactivating Protein [Luffa acutangula] >2OQA_B X-ray Sequence and Crystal Structure of Luffaculin 1, a Novel Type 1 Ribosome-inactivating Protein [Luffa acutangula])

HSP 1 Score: 422.2 bits (1084), Expect = 4.1e-114
Identity = 228/241 (94.61%), Postives = 231/241 (95.85%), Query Frame = 0

Query: 81  DVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 140
           DVSFSLSGSSSTSYSKFIG LRKALPS+GTVYNITLLLSSASGASRYTLMKLSNYDGKAI
Sbjct: 1   DVSFSLSGSSSTSYSKFIGALRKALPSNGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 60

Query: 141 TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQTAEGKI 200
           TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF GSTIVTLPYSGNYEKLQTA GKI
Sbjct: 61  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFAGSTIVTLPYSGNYEKLQTAAGKI 120

Query: 201 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERISKNGVP 260
           REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAE+SRFKYIEGQII RISKNGVP
Sbjct: 121 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAESSRFKYIEGQIIMRISKNGVP 180

Query: 261 SLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTKNIKLLL 320
           SLATISLENEWSALSKQIQL QTNNGTFKTPVVI D  GQRVEI NV SKVVTKNI+LLL
Sbjct: 181 SLATISLENEWSALSKQIQLAQTNNGTFKTPVVIMDAGGQRVEIGNVGSKVVTKNIQLLL 240

Query: 321 N 322
           N
Sbjct: 241 N 241

BLAST of Spg014631 vs. NCBI nr
Match: ABY71834.1 (ribosome-inactivating protein [Luffa acutangula] >CAA44230.1 b-luffin [Luffa aegyptiaca])

HSP 1 Score: 379.0 bits (972), Expect = 4.0e-101
Identity = 208/247 (84.21%), Postives = 219/247 (88.66%), Query Frame = 0

Query: 77  TVE-ADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLSNY 136
           TVE A+VSFSLSG+ S SYSKFI  LRKALPS   V NI LLL SASGASRY LM+LSNY
Sbjct: 18  TVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNY 77

Query: 137 DGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQT 196
           D KAIT+AIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYE+LQ 
Sbjct: 78  DAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQN 137

Query: 197 AEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERIS 256
           A GK+REKIPLGF A DSAIT+LFHYDSTAAA AFLVIIQTTAEASRFKYIEGQIIERI 
Sbjct: 138 AAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFKYIEGQIIERIP 197

Query: 257 KNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTKN 316
           KN VPS A +SLENEWSALSKQIQL QTNNG F+TPVVI D+KGQRVEI +V SKVVT N
Sbjct: 198 KNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEIKDVNSKVVTNN 257

Query: 317 IKLLLNK 323
           IKLLLNK
Sbjct: 258 IKLLLNK 264

BLAST of Spg014631 vs. NCBI nr
Match: 1NIO_A (Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolution [Luffa aegyptiaca])

HSP 1 Score: 377.1 bits (967), Expect = 1.5e-100
Identity = 204/243 (83.95%), Postives = 216/243 (88.89%), Query Frame = 0

Query: 80  ADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLSNYDGKA 139
           A+VSFSLSG+ S SYSKFI  LRKALPS   V NI LLL SASGASRY LM+LSNYD KA
Sbjct: 1   ANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKA 60

Query: 140 ITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQTAEGK 199
           IT+AIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYE+LQ A GK
Sbjct: 61  ITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGK 120

Query: 200 IREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERISKNGV 259
           +REKIPLGF A DSAIT+LFHYDSTAAA AFLVIIQTTAEASRFKYIEGQII+RI KN V
Sbjct: 121 VREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFKYIEGQIIKRIPKNEV 180

Query: 260 PSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTKNIKLL 319
           PS A +SLENEWSALSKQIQL QTNNG F+TPVVI D+KGQRVEI +V SKVVT NIKLL
Sbjct: 181 PSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEIKDVNSKVVTNNIKLL 240

Query: 320 LNK 323
           LNK
Sbjct: 241 LNK 243

BLAST of Spg014631 vs. NCBI nr
Match: XP_022953306.1 (ribosome-inactivating protein luffaculin 1-like [Cucurbita moschata])

HSP 1 Score: 370.2 bits (949), Expect = 1.9e-98
Identity = 197/248 (79.44%), Postives = 219/248 (88.31%), Query Frame = 0

Query: 74  TASTVEADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLS 133
           ++S V  DVSF+L GS+S SYS FI  LR ALPS   VYNI+LLL SA+GASRYT +KLS
Sbjct: 28  SSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGSATGASRYTQVKLS 87

Query: 134 NYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKL 193
           NYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVFK ST +TLPYSGNYEKL
Sbjct: 88  NYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRITLPYSGNYEKL 147

Query: 194 QTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIER 253
           Q A GK REKIPLGFPALD+AITTLFHYDSTAAAAAF+V+IQ TAEASR++YIE Q+I+R
Sbjct: 148 QNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAEASRYRYIEAQMIKR 207

Query: 254 ISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVT 313
           ISK+ VPSLA ISLEN WSALSKQIQL QTN G FK PV+ITDD+GQRVEITNV+SKVV 
Sbjct: 208 ISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRVEITNVSSKVVA 267

Query: 314 KNIKLLLN 322
           KNI+LLLN
Sbjct: 268 KNIQLLLN 275

BLAST of Spg014631 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 2.7e-124
Identity = 239/247 (96.76%), Postives = 242/247 (97.98%), Query Frame = 0

Query: 75  ASTVEADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLSN 134
           ASTVEADV FSLSGSSSTSYSKFIGDLRKALPS+GTVYNITLLLSSASGASRYTLM LSN
Sbjct: 15  ASTVEADVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLSSASGASRYTLMTLSN 74

Query: 135 YDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQ 194
           YDGKAITVA+DVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQ
Sbjct: 75  YDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQ 134

Query: 195 TAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERI 254
           TA GKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERI
Sbjct: 135 TAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERI 194

Query: 255 SKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTK 314
           SKN VPSLATISLENEWSALSKQIQL QTNNGTFKTPVVITDDKGQRVEITNVTSKVVTK
Sbjct: 195 SKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTK 254

Query: 315 NIKLLLN 322
           NI+LLLN
Sbjct: 255 NIQLLLN 261

BLAST of Spg014631 vs. ExPASy Swiss-Prot
Match: P84530 (Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=2)

HSP 1 Score: 422.2 bits (1084), Expect = 5.4e-117
Identity = 228/241 (94.61%), Postives = 231/241 (95.85%), Query Frame = 0

Query: 81  DVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 140
           DVSFSLSGSSSTSYSKFIG LRKALPS+GTVYNITLLLSSASGASRYTLMKLSNYDGKAI
Sbjct: 1   DVSFSLSGSSSTSYSKFIGALRKALPSNGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 60

Query: 141 TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQTAEGKI 200
           TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF GSTIVTLPYSGNYEKLQTA GKI
Sbjct: 61  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFAGSTIVTLPYSGNYEKLQTAAGKI 120

Query: 201 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERISKNGVP 260
           REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAE+SRFKYIEGQII RISKNGVP
Sbjct: 121 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAESSRFKYIEGQIIMRISKNGVP 180

Query: 261 SLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTKNIKLLL 320
           SLATISLENEWSALSKQIQL QTNNGTFKTPVVI D  GQRVEI NV SKVVTKNI+LLL
Sbjct: 181 SLATISLENEWSALSKQIQLAQTNNGTFKTPVVIMDAGGQRVEIGNVGSKVVTKNIQLLL 240

Query: 321 N 322
           N
Sbjct: 241 N 241

BLAST of Spg014631 vs. ExPASy Swiss-Prot
Match: P22851 (Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 8.7e-99
Identity = 200/246 (81.30%), Postives = 215/246 (87.40%), Query Frame = 0

Query: 80  ADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLSNYDGKA 139
           A+VSFSLSG+ S SYSKFI  LRKALPS   V NI LLL SASGASRY LM+LSNYD KA
Sbjct: 1   ANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKA 60

Query: 140 ITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQTAEGK 199
           IT+AIDVTNVYIMGYLVNSTSYF NESDAKLASQYVFKGST+VT+PYSGNYE+LQ A GK
Sbjct: 61  ITMAIDVTNVYIMGYLVNSTSYFANESDAKLASQYVFKGSTLVTIPYSGNYERLQNAAGK 120

Query: 200 IREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERISKNGV 259
           IREKIPLGF ALDSA+T++FHYDSTAAAAAFLVI+QTTAEASRFKYIEGQIIERI KN V
Sbjct: 121 IREKIPLGFRALDSALTSIFHYDSTAAAAAFLVILQTTAEASRFKYIEGQIIERIPKNEV 180

Query: 260 PSLATISLENE-WSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTK--NI 319
           PS A +SLENE WS LSKQIQL QTNNG F+TPVVI D+KGQRVEITN+ SKV  K  N 
Sbjct: 181 PSPAALSLENEAWSLLSKQIQLAQTNNGAFRTPVVIIDNKGQRVEITNLASKVQIKDVNS 240

Query: 320 KLLLNK 323
           KLLLNK
Sbjct: 241 KLLLNK 246

BLAST of Spg014631 vs. ExPASy Swiss-Prot
Match: P16094 (Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 324.3 bits (830), Expect = 1.5e-87
Identity = 176/247 (71.26%), Postives = 198/247 (80.16%), Query Frame = 0

Query: 76  STVEADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLSNY 135
           S V+ DVSF LSG+   SY  FI DLR ALP    VYNI LLL S SGA RY LM L NY
Sbjct: 19  SIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPLLLPSVSGAGRYLLMHLFNY 78

Query: 136 DGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGS-TIVTLPYSGNYEKLQ 195
           DGK ITVA+DVTNVYIMGYL ++TSYFFNE  A+LASQYVF+ +   +TLPYSGNYE+LQ
Sbjct: 79  DGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVFRDARRKITLPYSGNYERLQ 138

Query: 196 TAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERI 255
            A GK REKIP+G PALDSAI+TL HYDSTAAA A LV+IQTTAEA+RFKYIE QI ER 
Sbjct: 139 IAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQTTAEAARFKYIEQQIQERA 198

Query: 256 SKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTK 315
            ++ VPSLATISLEN WS LSKQIQL Q NNG F+TP+V+ D+KG RV+ITNVTSKVVT 
Sbjct: 199 YRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLVDNKGNRVQITNVTSKVVTS 258

Query: 316 NIKLLLN 322
           NI+LLLN
Sbjct: 259 NIQLLLN 265

BLAST of Spg014631 vs. ExPASy Swiss-Prot
Match: Q9FRX4 (Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.1e-84
Identity = 166/252 (65.87%), Postives = 204/252 (80.95%), Query Frame = 0

Query: 73  HTASTVEADVS--FSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLM 132
           H   T E DV+  FSL GS+  SYSKFI  +R ALP++G +YNI LL+ S SG+ RY LM
Sbjct: 17  HGVPTAEGDVTVKFSLLGSNHKSYSKFITSMRNALPNAGDIYNIPLLVPSISGSRRYILM 76

Query: 133 KLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNY 192
           +LSNY+G  IT+A+DVTNVYIMGYLVN TSYFFNE+DA+LAS++VF+G+  +TLPYSGNY
Sbjct: 77  QLSNYEGNTITMAVDVTNVYIMGYLVNGTSYFFNETDAQLASKFVFQGTKSITLPYSGNY 136

Query: 193 EKLQTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQI 252
           +KLQ+   K R+ IPLGF ALDSAI+TL++YDS +A  AFLV+IQTTAEA+R+KYIE QI
Sbjct: 137 QKLQSVARKERDSIPLGFMALDSAISTLYYYDSRSAPIAFLVLIQTTAEAARYKYIEKQI 196

Query: 253 IERISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSK 312
           I+RIS + VP LA ISLENEWS LSKQIQ+ ++NNG F+TPV I +DKG   E+TNV+S 
Sbjct: 197 IDRISVSKVPDLAAISLENEWSLLSKQIQIAKSNNGQFQTPVKIINDKGILTEVTNVSSL 256

Query: 313 VVTKNIKLLLNK 323
           VVTKNI LLLNK
Sbjct: 257 VVTKNIMLLLNK 268

BLAST of Spg014631 vs. ExPASy TrEMBL
Match: B0EVM6 (rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.9e-101
Identity = 208/247 (84.21%), Postives = 219/247 (88.66%), Query Frame = 0

Query: 77  TVE-ADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLSNY 136
           TVE A+VSFSLSG+ S SYSKFI  LRKALPS   V NI LLL SASGASRY LM+LSNY
Sbjct: 18  TVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNY 77

Query: 137 DGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQT 196
           D KAIT+AIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYE+LQ 
Sbjct: 78  DAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQN 137

Query: 197 AEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERIS 256
           A GK+REKIPLGF A DSAIT+LFHYDSTAAA AFLVIIQTTAEASRFKYIEGQIIERI 
Sbjct: 138 AAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFKYIEGQIIERIP 197

Query: 257 KNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTKN 316
           KN VPS A +SLENEWSALSKQIQL QTNNG F+TPVVI D+KGQRVEI +V SKVVT N
Sbjct: 198 KNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEIKDVNSKVVTNN 257

Query: 317 IKLLLNK 323
           IKLLLNK
Sbjct: 258 IKLLLNK 264

BLAST of Spg014631 vs. ExPASy TrEMBL
Match: Q00980 (rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.9e-101
Identity = 208/247 (84.21%), Postives = 219/247 (88.66%), Query Frame = 0

Query: 77  TVE-ADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLSNY 136
           TVE A+VSFSLSG+ S SYSKFI  LRKALPS   V NI LLL SASGASRY LM+LSNY
Sbjct: 18  TVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNY 77

Query: 137 DGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQT 196
           D KAIT+AIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYE+LQ 
Sbjct: 78  DAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQN 137

Query: 197 AEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERIS 256
           A GK+REKIPLGF A DSAIT+LFHYDSTAAA AFLVIIQTTAEASRFKYIEGQIIERI 
Sbjct: 138 AAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFKYIEGQIIERIP 197

Query: 257 KNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTKN 316
           KN VPS A +SLENEWSALSKQIQL QTNNG F+TPVVI D+KGQRVEI +V SKVVT N
Sbjct: 198 KNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEIKDVNSKVVTNN 257

Query: 317 IKLLLNK 323
           IKLLLNK
Sbjct: 258 IKLLLNK 264

BLAST of Spg014631 vs. ExPASy TrEMBL
Match: A0A6J1GMM2 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 9.0e-99
Identity = 197/248 (79.44%), Postives = 219/248 (88.31%), Query Frame = 0

Query: 74  TASTVEADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLS 133
           ++S V  DVSF+L GS+S SYS FI  LR ALPS   VYNI+LLL SA+GASRYT +KLS
Sbjct: 28  SSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGSATGASRYTQVKLS 87

Query: 134 NYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKL 193
           NYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVFK ST +TLPYSGNYEKL
Sbjct: 88  NYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRITLPYSGNYEKL 147

Query: 194 QTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIER 253
           Q A GK REKIPLGFPALD+AITTLFHYDSTAAAAAF+V+IQ TAEASR++YIE Q+I+R
Sbjct: 148 QNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAEASRYRYIEAQMIKR 207

Query: 254 ISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVT 313
           ISK+ VPSLA ISLEN WSALSKQIQL QTN G FK PV+ITDD+GQRVEITNV+SKVV 
Sbjct: 208 ISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRVEITNVSSKVVA 267

Query: 314 KNIKLLLN 322
           KNI+LLLN
Sbjct: 268 KNIQLLLN 275

BLAST of Spg014631 vs. ExPASy TrEMBL
Match: A0A6J1JVG7 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 1.2e-98
Identity = 198/248 (79.84%), Postives = 218/248 (87.90%), Query Frame = 0

Query: 74  TASTVEADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLS 133
           ++S V  DVSF+L GS+S SYS FI  LR ALPS   VYNI+LLL SA+GASRYT +KLS
Sbjct: 28  SSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISLLLGSATGASRYTQVKLS 87

Query: 134 NYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKL 193
           NYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVFK ST +TLPYSGNYEKL
Sbjct: 88  NYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRITLPYSGNYEKL 147

Query: 194 QTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIER 253
           Q A GK REKIPLGFPALDSAITTLFHYDSTAAAAAF+V+IQ TAEASR+KYIE Q+I+R
Sbjct: 148 QNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQCTAEASRYKYIEAQMIKR 207

Query: 254 ISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVT 313
           ISK+ VPS A ISLEN WSALSKQIQL QTN G FK PV+ITDD+GQRVEITNV+S VVT
Sbjct: 208 ISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRVEITNVSSNVVT 267

Query: 314 KNIKLLLN 322
           KNI+LLLN
Sbjct: 268 KNIQLLLN 275

BLAST of Spg014631 vs. ExPASy TrEMBL
Match: A0A6J1GMJ7 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455802 PE=3 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 2.3e-86
Identity = 177/250 (70.80%), Postives = 208/250 (83.20%), Query Frame = 0

Query: 73  HTASTVEADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKL 132
           HTA  V  ++SFSLSGSSS SYSKFI  LR ALP++G VYNI LLL +  G++RYTLMKL
Sbjct: 19  HTA--VGDNLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPLLLPNLKGSARYTLMKL 78

Query: 133 SNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEK 192
           SNY+ K ITVAIDVTNVYIMGYLVN+TSYFFNE  A+LAS++VF+G+  + LPYSGNY+K
Sbjct: 79  SNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVFQGTKHILLPYSGNYQK 138

Query: 193 LQTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIE 252
           LQ A  K R+ IPLGF ALD+AI+ L+HYD+ AA AAFLV+IQTTAEASRFKYIE QII+
Sbjct: 139 LQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQTTAEASRFKYIEKQIID 198

Query: 253 RISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVV 312
           RI +N VPSLA ISLENEWS LSKQIQ+  +NNGTF+TPV + +DKG  VE+TNV+S VV
Sbjct: 199 RIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVKLINDKGLSVEVTNVSSLVV 258

Query: 313 TKNIKLLLNK 323
           T NIKLLLNK
Sbjct: 259 TNNIKLLLNK 266

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q00465.12.0e-12196.76RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-g... [more]
P84530.24.1e-11494.61RecName: Full=Ribosome-inactivating protein luffaculin 1; AltName: Full=rRNA N-g... [more]
ABY71834.14.0e-10184.21ribosome-inactivating protein [Luffa acutangula] >CAA44230.1 b-luffin [Luffa aeg... [more]
1NIO_A1.5e-10083.95Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolu... [more]
XP_022953306.11.9e-9879.44ribosome-inactivating protein luffaculin 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q004652.7e-12496.76Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1[more]
P845305.4e-11794.61Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=... [more]
P228518.7e-9981.30Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1[more]
P160941.5e-8771.26Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=... [more]
Q9FRX42.1e-8465.87Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
B0EVM61.9e-10184.21rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1[more]
Q009801.9e-10184.21rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
A0A6J1GMM29.0e-9979.44rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1[more]
A0A6J1JVG71.2e-9879.84rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1[more]
A0A6J1GMJ72.3e-8670.80rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455802 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017989Ribosome-inactivating protein type 1/2PRINTSPR00396SHIGARICINcoord: 139..154
score: 52.3
coord: 201..215
score: 56.84
coord: 230..250
score: 58.15
coord: 92..105
score: 45.3
coord: 262..279
score: 52.34
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 90..279
e-value: 1.4E-29
score: 103.2
IPR001574Ribosome-inactivating proteinPANTHERPTHR33453FAMILY NOT NAMEDcoord: 78..318
IPR016139Ribosome-inactivating protein, subdomain 2GENE3D4.10.470.10Ricin (A Subunit), domain 2coord: 242..322
e-value: 2.3E-27
score: 96.6
IPR016138Ribosome-inactivating protein, subdomain 1GENE3D3.40.420.10Ricin (A subunit), domain 1coord: 80..241
e-value: 2.5E-44
score: 152.8
NoneNo IPR availablePANTHERPTHR33453:SF14RRNA N-GLYCOSIDASEcoord: 78..318
IPR017988Ribosome-inactivating protein conserved sitePROSITEPS00275SHIGA_RICINcoord: 234..250
IPR036041Ribosome-inactivating protein superfamilySUPERFAMILY56371Ribosome inactivating proteins (RIP)coord: 81..321

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg014631.1Spg014631.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051607 defense response to virus
biological_process GO:0035821 modulation of process of other organism
biological_process GO:0017148 negative regulation of translation
molecular_function GO:0030598 rRNA N-glycosylase activity
molecular_function GO:0090729 toxin activity