Spg013529 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg013529
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionPPM-type phosphatase domain-containing protein
Locationscaffold1: 13958390 .. 13961657 (+)
RNA-Seq ExpressionSpg013529
SyntenySpg013529
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAGATTTCAAACATTTGACTTTTGGATTTTACTAAGAATGTACAAAAAATTATTAGAAAATTTTTCCTTATTTTAAGAAGGGAAAAAAAATAATAATAATAGAACCATTTCTTTAACTAAAAATTCTAAAATTCTCCACTTTGTTTGCTTTTTCTTTAACGGAGGGTCAACGGCCTAATAAATACAAAAAAAAATAAAAGCACTAAGTATTTTTTAGAAAAAAAAAAGGAGATTAAAAAAGCAAGAAACAAACAAAGCCCACTTCCAAAAGTCAACTCCTCTCTCTCTCTCACTTCTTTCTCTTTCTCTCTCATCAAAAATTTCTCAGCTATCAAACTTCTTCTTTCTCTTTCACGCGCACTCACACTGAGAGAGAGTATTATGCCATGATCAATCAAATCAAACAGTAGAGAAAGATAAATTCTCTTTTTTTTTCTTTTCTTTTTTCATATGGGAAATCGAATTGGGAAAATTGGCCACTGCTTCGCCGGCGACGGCGATATCTCCCGCCGGTATGATCCCGCCGACTATACAGCCGACCCCAACGACGGCGGCTTCGGCCACTCCTTCTACTACCTTCCACCCGACCCACATACTTTCTCCTCCTCCAAAGATTTCTCCGAAGACTACATCTCACCGGCCACGATTTTCCGGTCAATTTCCGGCGCCTCTGTAAGCGCCAACGTCTCAACGCCGCTCTCCACTTCTCTCGTCGATCTTTATCCTTACAGCACCACGTTTGATCGGGCTGCCGCCTTTGATTGTTCGAATTCCTTCGCCTCCGTGCCGCTGCAGCCGGTGCCCCGCCACTCGGTGTCGGGTAATTCCGGCGCTTCGGGTCCGATCGAGAGAGGGTTCTTATCCGGACCCTTGGCTGCCCGGAGCTTCGAATCTGGTCCGATTGATCGAGGGGTTTATTCCGGCCCGATTGAAAAGGGCGGCGGACCGGAGAAATTGCAGAGGAGCTTCTCCCATGGCGGCGTTGGAGATAGACAACCCAAATCGAAAAGAAGAAGTCTCATTCGGATTCTGAAGAGAGCCATATCCAAAACCATTTCTCGAAACCAGAAGGCTGTGAGTTCGAGAGTGAGAGAATCGGAATCGTACAAACACAATGAAAATGCAGCTGCAGCAGCTCAGACTCAGAGCAGTGTTCATTTGAGCAGCCACGCGAGCTTGGCGCAGGAAGACGATGGCGATTACTTTCTGGGCGGTCAGAGCGTACAGTGGGCACAGGGGAAGGCCGGTGAGGACAGAGTTCACGTGGTGATATCGGAGGATAATGGTTGGGTTTTCGTTGGAATTTACGATGGATTCAACGGCCCTGATGCTCCTGATTACTTACTCGCTAATCTCTACACTGCCGTTCTCAAAGAACTCAAGGGCTTGATTTGGAATGATAAGTTTGATTCAACTGCAACTTCGTCTTCCATGAACTCATCAAACTCTGCTTCAATTGAGGAAGATGATGGAACAACCCACATGAAGAATCAAGGGAATGATCATCAATCTACACAGGTCGCAAATGGGATTGTGAATTTGGAGGCCGATTCCGCGAGGAAAAGAGAGAGAGTTTATAATACAAACTATAGGACGAGTCAGCTGATGAAGAAGGCTGATCAGAATCCTAAGAATTGGAAATGTGAATGGGAAAGGGAAAGATTAGAATTTGATGGGAAGTTGAACAATCGACATTTAGATCATCACCAAATATGGGATGAATCGACAGCAATCAATCATTCTGAAGTGTTGAGTGCTCTGTCTCAAGCATTGAGAAAGACAGAGGAAGCATATCTGCAGAATGCAGACAAGATGGTTACAAGAAATCCTGAGCTAGCTCTTATGGGCTCTTGTGTTTTGGTTATGTTGATGAGAGGAGAAGATGTCTACTTGATGAATGTAGGAGACAGCCGTGCAATTGTAGCGCAGCAACTCGAACCGGATTTTGGGATTGGAAGAATCAATGAAGGAACACTACGAGTGTTCGAATCCTCAAATGGAGTAGAGTTTGAGAGATTTACAACCTTGGCTTCTCATCAGCTTACGATGGACCACAGCACGTACACAGAAGAGGTAGTGTTCTTGTCTATCTATATCACTCATTTTCTACACAAGACTGCATTTTTCATGTCCACTTTCTCTTTGGTCACAAGTTTTTTAGATTACATGAACTTATGAGAGAACACTTGAATATCAGGAAGTTCAAAGGATCAAGAATGCTCATCCCGATGACACTTCTGCAATTATGAATGACAGGGTGAAAGGTTATTTGAAAATCACACGGGCGTTCGGAGCTGGCTTTCTCAAACAGGTATTCCACATTTCTAATCCTTTTGAGTGAGCTAGAACGTAAGAGAAACATAGTCTGTCCAGTGTTATTCAACCAAAAACTAATCTGAGGCAGTACAGAAAATACAAGGCAAATGGTCTATTCAGTTCAGAATAGAGTGTTCTGTAAAATTTGAATGTTGTGCTGATCAGCTGAGTGTTTTGCAGCCAAAATGGAATGATGCATTGCTAGAGATGTTCAGAATAGACTATGTGGGAAATTCACCTTACATCACCTGTGATCCAACATTGTGCCATTATAAACTCAGCCCAACAGACAGATTCTTGATATTGTCCTCTGATGGACTCTACCAGTACTTCACCAATGAAGAAGCTGTTGCTCATGTAGAGTCCTTCATTGCTTCTTTTCCTGAAGGAGATCCTGCTCAAAATCTCATTGAAGAAGTATTGTTCCGAGCAGCGAAAAAATACGGTAAGTTTCTCATCTGTCTACAAGATAGGAAGACAACAGAACTAACAGATATCTATTGGAGTGTGAACTTTTACTAATGTGAGATTTGGAAAACTTGCAGGCATGGACTTCCATGAGCTGCTTGATATCCCACAAGGGGAGCGGCGGAAGTACCATGACGACGTTTCGGTAATCATCATTTCATTTGAAGGAAGAATTTGGCATTCATTGATGTAAATAGAGATACTAGCCTATAGAAATCAAGATAACAGAAGGTTGGGCAATTAATTCTGCCATTTATTTCATTTATTCTTCCCTATTCTCATTACACATGCAATCAAATCAATCTCTCTTCTTCAACCTTTAACAGAGAGAGTGTAGAGTTATTCAGCTTCCTGTCTTCACTCCAATTGTGTGCGTCTGTGTTGCATCAAATACCAATACATTCCAAAGCAGACCTGTTTGGAAAATTTCAACAATTTTGTTTTGCAGACTTCAGAAGTTTTTCTCTAGAAGTTTTGACTTCC

mRNA sequence

ATGGGAAATCGAATTGGGAAAATTGGCCACTGCTTCGCCGGCGACGGCGATATCTCCCGCCGGTATGATCCCGCCGACTATACAGCCGACCCCAACGACGGCGGCTTCGGCCACTCCTTCTACTACCTTCCACCCGACCCACATACTTTCTCCTCCTCCAAAGATTTCTCCGAAGACTACATCTCACCGGCCACGATTTTCCGGTCAATTTCCGGCGCCTCTGTAAGCGCCAACGTCTCAACGCCGCTCTCCACTTCTCTCGTCGATCTTTATCCTTACAGCACCACGTTTGATCGGGCTGCCGCCTTTGATTGTTCGAATTCCTTCGCCTCCGTGCCGCTGCAGCCGGTGCCCCGCCACTCGGTGTCGGGTAATTCCGGCGCTTCGGGTCCGATCGAGAGAGGGTTCTTATCCGGACCCTTGGCTGCCCGGAGCTTCGAATCTGGTCCGATTGATCGAGGGGTTTATTCCGGCCCGATTGAAAAGGGCGGCGGACCGGAGAAATTGCAGAGGAGCTTCTCCCATGGCGGCGTTGGAGATAGACAACCCAAATCGAAAAGAAGAAGTCTCATTCGGATTCTGAAGAGAGCCATATCCAAAACCATTTCTCGAAACCAGAAGGCTGTGAGTTCGAGAGTGAGAGAATCGGAATCGTACAAACACAATGAAAATGCAGCTGCAGCAGCTCAGACTCAGAGCAGTGTTCATTTGAGCAGCCACGCGAGCTTGGCGCAGGAAGACGATGGCGATTACTTTCTGGGCGGTCAGAGCGTACAGTGGGCACAGGGGAAGGCCGGTGAGGACAGAGTTCACGTGGTGATATCGGAGGATAATGGTTGGGTTTTCGTTGGAATTTACGATGGATTCAACGGCCCTGATGCTCCTGATTACTTACTCGCTAATCTCTACACTGCCGTTCTCAAAGAACTCAAGGGCTTGATTTGGAATGATAAGTTTGATTCAACTGCAACTTCGTCTTCCATGAACTCATCAAACTCTGCTTCAATTGAGGAAGATGATGGAACAACCCACATGAAGAATCAAGGGAATGATCATCAATCTACACAGGTCGCAAATGGGATTGTGAATTTGGAGGCCGATTCCGCGAGGAAAAGAGAGAGAGTTTATAATACAAACTATAGGACGAGTCAGCTGATGAAGAAGGCTGATCAGAATCCTAAGAATTGGAAATGTGAATGGGAAAGGGAAAGATTAGAATTTGATGGGAAGTTGAACAATCGACATTTAGATCATCACCAAATATGGGATGAATCGACAGCAATCAATCATTCTGAAGTGTTGAGTGCTCTGTCTCAAGCATTGAGAAAGACAGAGGAAGCATATCTGCAGAATGCAGACAAGATGGTTACAAGAAATCCTGAGCTAGCTCTTATGGGCTCTTGTGTTTTGGTTATGTTGATGAGAGGAGAAGATGTCTACTTGATGAATGTAGGAGACAGCCGTGCAATTGTAGCGCAGCAACTCGAACCGGATTTTGGGATTGGAAGAATCAATGAAGGAACACTACGAGTGTTCGAATCCTCAAATGGAGTAGAGTTTGAGAGATTTACAACCTTGGCTTCTCATCAGCTTACGATGGACCACAGCACGTACACAGAAGAGGAAGTTCAAAGGATCAAGAATGCTCATCCCGATGACACTTCTGCAATTATGAATGACAGGGTGAAAGGTTATTTGAAAATCACACGGGCGTTCGGAGCTGGCTTTCTCAAACAGTACTTCACCAATGAAGAAGCTGTTGCTCATGTAGAGTCCTTCATTGCTTCTTTTCCTGAAGGAGATCCTGCTCAAAATCTCATTGAAGAAGTATTGTTCCGAGCAGCGAAAAAATACGGCATGGACTTCCATGAGCTGCTTGATATCCCACAAGGGGAGCGGCGGAAGTACCATGACGACGTTTCGGTAATCATCATTTCATTTGAAGGAAGAATTTGGCATTCATTGATGTAA

Coding sequence (CDS)

ATGGGAAATCGAATTGGGAAAATTGGCCACTGCTTCGCCGGCGACGGCGATATCTCCCGCCGGTATGATCCCGCCGACTATACAGCCGACCCCAACGACGGCGGCTTCGGCCACTCCTTCTACTACCTTCCACCCGACCCACATACTTTCTCCTCCTCCAAAGATTTCTCCGAAGACTACATCTCACCGGCCACGATTTTCCGGTCAATTTCCGGCGCCTCTGTAAGCGCCAACGTCTCAACGCCGCTCTCCACTTCTCTCGTCGATCTTTATCCTTACAGCACCACGTTTGATCGGGCTGCCGCCTTTGATTGTTCGAATTCCTTCGCCTCCGTGCCGCTGCAGCCGGTGCCCCGCCACTCGGTGTCGGGTAATTCCGGCGCTTCGGGTCCGATCGAGAGAGGGTTCTTATCCGGACCCTTGGCTGCCCGGAGCTTCGAATCTGGTCCGATTGATCGAGGGGTTTATTCCGGCCCGATTGAAAAGGGCGGCGGACCGGAGAAATTGCAGAGGAGCTTCTCCCATGGCGGCGTTGGAGATAGACAACCCAAATCGAAAAGAAGAAGTCTCATTCGGATTCTGAAGAGAGCCATATCCAAAACCATTTCTCGAAACCAGAAGGCTGTGAGTTCGAGAGTGAGAGAATCGGAATCGTACAAACACAATGAAAATGCAGCTGCAGCAGCTCAGACTCAGAGCAGTGTTCATTTGAGCAGCCACGCGAGCTTGGCGCAGGAAGACGATGGCGATTACTTTCTGGGCGGTCAGAGCGTACAGTGGGCACAGGGGAAGGCCGGTGAGGACAGAGTTCACGTGGTGATATCGGAGGATAATGGTTGGGTTTTCGTTGGAATTTACGATGGATTCAACGGCCCTGATGCTCCTGATTACTTACTCGCTAATCTCTACACTGCCGTTCTCAAAGAACTCAAGGGCTTGATTTGGAATGATAAGTTTGATTCAACTGCAACTTCGTCTTCCATGAACTCATCAAACTCTGCTTCAATTGAGGAAGATGATGGAACAACCCACATGAAGAATCAAGGGAATGATCATCAATCTACACAGGTCGCAAATGGGATTGTGAATTTGGAGGCCGATTCCGCGAGGAAAAGAGAGAGAGTTTATAATACAAACTATAGGACGAGTCAGCTGATGAAGAAGGCTGATCAGAATCCTAAGAATTGGAAATGTGAATGGGAAAGGGAAAGATTAGAATTTGATGGGAAGTTGAACAATCGACATTTAGATCATCACCAAATATGGGATGAATCGACAGCAATCAATCATTCTGAAGTGTTGAGTGCTCTGTCTCAAGCATTGAGAAAGACAGAGGAAGCATATCTGCAGAATGCAGACAAGATGGTTACAAGAAATCCTGAGCTAGCTCTTATGGGCTCTTGTGTTTTGGTTATGTTGATGAGAGGAGAAGATGTCTACTTGATGAATGTAGGAGACAGCCGTGCAATTGTAGCGCAGCAACTCGAACCGGATTTTGGGATTGGAAGAATCAATGAAGGAACACTACGAGTGTTCGAATCCTCAAATGGAGTAGAGTTTGAGAGATTTACAACCTTGGCTTCTCATCAGCTTACGATGGACCACAGCACGTACACAGAAGAGGAAGTTCAAAGGATCAAGAATGCTCATCCCGATGACACTTCTGCAATTATGAATGACAGGGTGAAAGGTTATTTGAAAATCACACGGGCGTTCGGAGCTGGCTTTCTCAAACAGTACTTCACCAATGAAGAAGCTGTTGCTCATGTAGAGTCCTTCATTGCTTCTTTTCCTGAAGGAGATCCTGCTCAAAATCTCATTGAAGAAGTATTGTTCCGAGCAGCGAAAAAATACGGCATGGACTTCCATGAGCTGCTTGATATCCCACAAGGGGAGCGGCGGAAGTACCATGACGACGTTTCGGTAATCATCATTTCATTTGAAGGAAGAATTTGGCATTCATTGATGTAA

Protein sequence

MGNRIGKIGHCFAGDGDISRRYDPADYTADPNDGGFGHSFYYLPPDPHTFSSSKDFSEDYISPATIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPRHSVSGNSGASGPIERGFLSGPLAARSFESGPIDRGVYSGPIEKGGGPEKLQRSFSHGGVGDRQPKSKRRSLIRILKRAISKTISRNQKAVSSRVRESESYKHNENAAAAAQTQSSVHLSSHASLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAPDYLLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEDDGTTHMKNQGNDHQSTQVANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLEFDGKLNNRHLDHHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLVMLMRGEDVYLMNVGDSRAIVAQQLEPDFGIGRINEGTLRVFESSNGVEFERFTTLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGFLKQYFTNEEAVAHVESFIASFPEGDPAQNLIEEVLFRAAKKYGMDFHELLDIPQGERRKYHDDVSVIIISFEGRIWHSLM
Homology
BLAST of Spg013529 vs. NCBI nr
Match: XP_038877629.1 (probable protein phosphatase 2C 23 [Benincasa hispida])

HSP 1 Score: 1146.3 bits (2964), Expect = 0.0e+00
Identity = 613/725 (84.55%), Postives = 629/725 (86.76%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGDGDISRRYDPADYTADPNDGGFGHSFYYLPPDPHTFSSSKDFSEDY 60
           MGNRIGKIGHCFAG GDISRRYDPAD  +DP+DGGFGHSFYYLPPDPHT SSSKDFS+DY
Sbjct: 1   MGNRIGKIGHCFAGAGDISRRYDPADIISDPHDGGFGHSFYYLPPDPHTLSSSKDFSDDY 60

Query: 61  ISPATIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPRH 120
           ISP TIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPRH
Sbjct: 61  ISPTTIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPRH 120

Query: 121 SVSGNSG---ASGPIERGFLSGPLAARSFESGPIDRGVYSGPIEKGGGPEKLQRSFSHGG 180
           SVSGNSG   +SGP+ERGFLSGPLAARSFESGPIDR VYSGPIEKGGG EKLQRS SHGG
Sbjct: 121 SVSGNSGGFPSSGPLERGFLSGPLAARSFESGPIDRAVYSGPIEKGGGAEKLQRSVSHGG 180

Query: 181 VGDRQPKSK---RRSLIRILKRAISKTISRNQKAVSSRVRESESYKHNENAAA-AAQTQS 240
            GDR+PK K   RR+LIRILKRAISKTIS NQKA SSRVRESESYKHNENAAA AAQTQS
Sbjct: 181 DGDREPKLKKRRRRNLIRILKRAISKTISLNQKAASSRVRESESYKHNENAAASAAQTQS 240

Query: 241 SVHLSSHASLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPD 300
           SVHLSSH SLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPD
Sbjct: 241 SVHLSSHGSLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPD 300

Query: 301 APDYLLANLYTAVLKELK-GLIWNDKFDSTATSSSMNSSNSASIEEDD-GTTHMKNQGND 360
           APDYLLANLYTAVLKELK GLIW+DKFDSTATSSSMNSSNSASIEEDD GT HMKN+ ND
Sbjct: 301 APDYLLANLYTAVLKELKGGLIWSDKFDSTATSSSMNSSNSASIEEDDHGTPHMKNREND 360

Query: 361 HQSTQ---VANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLEFD 420
           HQST+   V NG+VNLEADS +KR+R+YNTNYRTSQLMKKADQNPKNWKCEWERERL  +
Sbjct: 361 HQSTEIFPVENGVVNLEADSVKKRDRIYNTNYRTSQLMKKADQNPKNWKCEWERERLGLE 420

Query: 421 GKL-NNRHLDHHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELALMGS 480
           GKL NNR LDHHQIWDESTAINHSEVL+ALSQALRKTEEAYLQNADKMVTRNPELALMGS
Sbjct: 421 GKLNNNRQLDHHQIWDESTAINHSEVLNALSQALRKTEEAYLQNADKMVTRNPELALMGS 480

Query: 481 CVLVMLMRGEDVYLMNVGDSRAIVAQQLEPDFGIG-------RINEGTLRVFESSNGVEF 540
           CVLVMLMRGEDVYLMNVGDSRAIVAQQ EPDFG G       RINEGTLRVFESSNGVEF
Sbjct: 481 CVLVMLMRGEDVYLMNVGDSRAIVAQQFEPDFGTGKLYRDLERINEGTLRVFESSNGVEF 540

Query: 541 ERFTTLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGFLK-- 600
           ER   LASHQLTMDHSTYTEEEVQRIKNAHPDD SAIMNDRVKGYLKITRAFGAGFLK  
Sbjct: 541 ERLKALASHQLTMDHSTYTEEEVQRIKNAHPDDASAIMNDRVKGYLKITRAFGAGFLKQP 600

Query: 601 -----------------------------------------------QYFTNEEAVAHVE 657
                                                          QYFTNEEAVA VE
Sbjct: 601 KWNDALLEMFRIDYVGNSPYITCDPTLCHYKLSPTDRFLILSSDGLYQYFTNEEAVAQVE 660

BLAST of Spg013529 vs. NCBI nr
Match: XP_023518015.1 (probable protein phosphatase 2C 23 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1134.4 bits (2933), Expect = 0.0e+00
Identity = 608/721 (84.33%), Postives = 628/721 (87.10%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGDGDISRRYDPADYTADPNDGGFGHSFYYLPPDPHTFSSSKDFSEDY 60
           MGNRIGKIG CFAGDGDISRRYDPAD   DP+DGGFGHSFYYLPPDPHT SSSKDFS++Y
Sbjct: 1   MGNRIGKIGRCFAGDGDISRRYDPADIIVDPHDGGFGHSFYYLPPDPHTISSSKDFSDEY 60

Query: 61  ISPA-TIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR 120
           ISPA TIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR
Sbjct: 61  ISPATTIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR 120

Query: 121 HSVSGNSGA---SGPIERGFLSGPLAARSFESGPIDRGVYSGPIEKGG-GPEKLQRSFSH 180
           HSVSGNSG    SGPIERGFLSGPLA+R+ ESGPIDRGV SGPIEK G GPEKL+RSFSH
Sbjct: 121 HSVSGNSGGFPYSGPIERGFLSGPLASRN-ESGPIDRGVCSGPIEKSGVGPEKLRRSFSH 180

Query: 181 GGVGDRQPKSKRRSLIRILKRAISKTISRNQKAVSSRVRESESYKHNENAAAAAQTQSSV 240
            GVGDRQPKSKRRSLIRILK+AISKTISRNQKAVSSRVRESESYKHNEN AAA QTQSSV
Sbjct: 181 SGVGDRQPKSKRRSLIRILKKAISKTISRNQKAVSSRVRESESYKHNEN-AAATQTQSSV 240

Query: 241 HLSSHASLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP 300
           HLSSHASLA EDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP
Sbjct: 241 HLSSHASLAPEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP 300

Query: 301 DYLLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEDDGTTHMKNQGNDHQST 360
           DYLLANLY AVLKELKGLIWNDKFDSTATSSSMNSSNSASIEE+DGTTH+KN    HQST
Sbjct: 301 DYLLANLYAAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEEDGTTHLKN----HQST 360

Query: 361 Q---VANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLEFDGKLN 420
           +     N +VNLEA+S RKRERVYNTNYRTSQLMK+ADQNPK WKCEWERERLEFDGK+N
Sbjct: 361 ENFPAGNKMVNLEAESMRKRERVYNTNYRTSQLMKRADQNPKTWKCEWERERLEFDGKMN 420

Query: 421 NRHLD-HHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV 480
           N+ LD HHQIWDESTAINHSEVL+ALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV
Sbjct: 421 NKQLDHHHQIWDESTAINHSEVLNALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV 480

Query: 481 MLMRGEDVYLMNVGDSRAIVAQQLEPDFGIG-------RINEGTLRVFESSNGVEFERFT 540
           MLMRGEDVYLMNVGDSRAIVAQQLEPDFGIG       RINEGTLRVFESSNGVEFER T
Sbjct: 481 MLMRGEDVYLMNVGDSRAIVAQQLEPDFGIGKQYRDLERINEGTLRVFESSNGVEFERLT 540

Query: 541 TLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGFLK------ 600
           TLASHQLTMDHSTYTEEEVQRIK AHP+D SAIMNDRVKGYLKITRAFGAGFLK      
Sbjct: 541 TLASHQLTMDHSTYTEEEVQRIKKAHPNDASAIMNDRVKGYLKITRAFGAGFLKQPKWND 600

Query: 601 -------------------------------------------QYFTNEEAVAHVESFIA 657
                                                      QYFTNEEAV+ VESFIA
Sbjct: 601 ALLEMFRIDYVGNSPYITCDPTLCHYKLSPTDRFLILSSDGLYQYFTNEEAVSQVESFIA 660

BLAST of Spg013529 vs. NCBI nr
Match: XP_022966555.1 (probable protein phosphatase 2C 23 [Cucurbita maxima])

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 606/721 (84.05%), Postives = 628/721 (87.10%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGDGDISRRYDPADYTADPNDGGFGHSFYYLPPDPHTFSSSKDFSEDY 60
           MGNRIGKIG CFAGDGDISRRYDPAD   DP+DGGFGHSFYYLPPDPHT SSSKDFS++Y
Sbjct: 1   MGNRIGKIGRCFAGDGDISRRYDPADIIVDPHDGGFGHSFYYLPPDPHTISSSKDFSDEY 60

Query: 61  ISPA-TIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR 120
           ISPA TIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR
Sbjct: 61  ISPATTIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR 120

Query: 121 HSVSGNSGA---SGPIERGFLSGPLAARSFESGPIDRGVYSGPIEKGG-GPEKLQRSFSH 180
           HSVSGNSG    SGPIERGFLSGPLA+R+ ESGPIDRGV SGPIEK G GPEKL+RSFSH
Sbjct: 121 HSVSGNSGGFPYSGPIERGFLSGPLASRN-ESGPIDRGVCSGPIEKSGVGPEKLRRSFSH 180

Query: 181 GGVGDRQPKSKRRSLIRILKRAISKTISRNQKAVSSRVRESESYKHNENAAAAAQTQSSV 240
            GVGDRQPKSKRRSLIRILK+AISKTISRNQKAVSSRVRESESYKHNEN AAA QTQSSV
Sbjct: 181 SGVGDRQPKSKRRSLIRILKKAISKTISRNQKAVSSRVRESESYKHNEN-AAATQTQSSV 240

Query: 241 HLSSHASLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP 300
           HLSSHASLA EDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP
Sbjct: 241 HLSSHASLAPEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP 300

Query: 301 DYLLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEDDGTTHMKNQGNDHQST 360
           DYLLANLY AVL+ELKGLIW+DKFDSTATSSS+NSSNSASIEEDDGTTH+KN    HQST
Sbjct: 301 DYLLANLYAAVLRELKGLIWSDKFDSTATSSSVNSSNSASIEEDDGTTHLKN----HQST 360

Query: 361 Q---VANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLEFDGKLN 420
           +     N +VNLEA+S RKRERVYNTNYRTSQLMK+ADQNPK WKCEWERERLEFDGK+N
Sbjct: 361 ENFPAGNKMVNLEAESMRKRERVYNTNYRTSQLMKRADQNPKTWKCEWERERLEFDGKMN 420

Query: 421 NRHLD-HHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV 480
           N+ LD HHQIWDESTAINHSEVL+ALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV
Sbjct: 421 NKQLDHHHQIWDESTAINHSEVLNALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV 480

Query: 481 MLMRGEDVYLMNVGDSRAIVAQQLEPDFGIG-------RINEGTLRVFESSNGVEFERFT 540
           MLMRGEDVYLMNVGDSRAIVAQQLEPDFGIG       RINEGTLRVFESSNGVEFER T
Sbjct: 481 MLMRGEDVYLMNVGDSRAIVAQQLEPDFGIGKQYRDLERINEGTLRVFESSNGVEFERLT 540

Query: 541 TLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGFLK------ 600
           TLASHQLTMDHSTYTEEEVQRIK AHP+D SAIMNDRVKGYLKITRAFGAGFLK      
Sbjct: 541 TLASHQLTMDHSTYTEEEVQRIKKAHPNDASAIMNDRVKGYLKITRAFGAGFLKQPKWND 600

Query: 601 -------------------------------------------QYFTNEEAVAHVESFIA 657
                                                      QYFTNEEAV+ VESFIA
Sbjct: 601 ALLEMFRIDYVGNSPYITCDPTLCHYKLSPTDRFLILSSDGLYQYFTNEEAVSQVESFIA 660

BLAST of Spg013529 vs. NCBI nr
Match: KAG7027333.1 (putative protein phosphatase 2C 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1129.4 bits (2920), Expect = 0.0e+00
Identity = 605/721 (83.91%), Postives = 626/721 (86.82%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGDGDISRRYDPADYTADPNDGGFGHSFYYLPPDPHTFSSSKDFSEDY 60
           MGNRIGKIG CFAGDGDISRRYDPAD   DP+DGGFGHSFYYLPPDPHT SSSKDFS++Y
Sbjct: 1   MGNRIGKIGRCFAGDGDISRRYDPADIIVDPHDGGFGHSFYYLPPDPHTISSSKDFSDEY 60

Query: 61  ISPA-TIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR 120
           ISPA TIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR
Sbjct: 61  ISPATTIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR 120

Query: 121 HSVSGNSGA---SGPIERGFLSGPLAARSFESGPIDRGVYSGPIEKGG-GPEKLQRSFSH 180
           HSVSGNSG    SGPIERGFLSGPLA+R+ ESGPIDRGV SGPIEK G GPEKL+RSFSH
Sbjct: 121 HSVSGNSGGFPYSGPIERGFLSGPLASRN-ESGPIDRGVCSGPIEKSGVGPEKLRRSFSH 180

Query: 181 GGVGDRQPKSKRRSLIRILKRAISKTISRNQKAVSSRVRESESYKHNENAAAAAQTQSSV 240
            GVGDRQPKSKRRSLIRILK+AISKTISRNQKAVSSRVRESESYKHNEN AAA QTQSSV
Sbjct: 181 SGVGDRQPKSKRRSLIRILKKAISKTISRNQKAVSSRVRESESYKHNEN-AAATQTQSSV 240

Query: 241 HLSSHASLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP 300
           HLSSHASLA EDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP
Sbjct: 241 HLSSHASLAPEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP 300

Query: 301 DYLLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEDDGTTHMKNQGNDHQST 360
           DYLLANLY AVLKELKGLIWNDKFDSTATSSSMNSSNSASIEE+DG TH++N    HQST
Sbjct: 301 DYLLANLYAAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEEDGLTHLRN----HQST 360

Query: 361 Q---VANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLEFDGKLN 420
           +     N +VNLEA+S RKRERVYNTNYRTSQLMK+ADQNPK WKCEWERERLEFDGK+N
Sbjct: 361 ENFPAGNKMVNLEAESMRKRERVYNTNYRTSQLMKRADQNPKTWKCEWERERLEFDGKMN 420

Query: 421 NRHLD-HHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV 480
           N+ LD HHQIWDESTAINHSEVL+ALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV
Sbjct: 421 NKQLDHHHQIWDESTAINHSEVLNALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV 480

Query: 481 MLMRGEDVYLMNVGDSRAIVAQQLEPDFGIG-------RINEGTLRVFESSNGVEFERFT 540
           MLMRGEDVYLMNVGDSRAIVAQQLEPDFG G       RINEGTLRVFESSNGVEFER T
Sbjct: 481 MLMRGEDVYLMNVGDSRAIVAQQLEPDFGFGKQYRDLERINEGTLRVFESSNGVEFERLT 540

Query: 541 TLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGFLK------ 600
           TLASHQLTMDHSTYTEEEVQRIK AHP+D SAIMNDRVKGYLKITRAFGAGFLK      
Sbjct: 541 TLASHQLTMDHSTYTEEEVQRIKKAHPNDASAIMNDRVKGYLKITRAFGAGFLKQPKWND 600

Query: 601 -------------------------------------------QYFTNEEAVAHVESFIA 657
                                                      QYFTNEEAV+ VESFIA
Sbjct: 601 ALLEMFRIDYVGNSPYITCDPTLCHYKLSPTDRFLILSSDGLYQYFTNEEAVSQVESFIA 660

BLAST of Spg013529 vs. NCBI nr
Match: KAG6595324.1 (putative protein phosphatase 2C 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 605/721 (83.91%), Postives = 625/721 (86.69%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGDGDISRRYDPADYTADPNDGGFGHSFYYLPPDPHTFSSSKDFSEDY 60
           MGNRIGKIG CFAGDGDISRRYDPAD   DP+DGGFGHSFYYLPPDPHT SSSKDFS++Y
Sbjct: 1   MGNRIGKIGRCFAGDGDISRRYDPADIIVDPHDGGFGHSFYYLPPDPHTISSSKDFSDEY 60

Query: 61  ISPA-TIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR 120
           ISPA TIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR
Sbjct: 61  ISPATTIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR 120

Query: 121 HSVSGNSGA---SGPIERGFLSGPLAARSFESGPIDRGVYSGPIEKGG-GPEKLQRSFSH 180
           HSVSGNSG    SGPIERGFLSGPLA+R+ ESGPIDRGV SGPIEK G GPEKL+RSFSH
Sbjct: 121 HSVSGNSGGFPYSGPIERGFLSGPLASRN-ESGPIDRGVCSGPIEKSGVGPEKLRRSFSH 180

Query: 181 GGVGDRQPKSKRRSLIRILKRAISKTISRNQKAVSSRVRESESYKHNENAAAAAQTQSSV 240
            GVGDRQPKSKRRSLIRILK+AISKTISRNQKAVSSRVRESESYKHNEN AAA QTQSSV
Sbjct: 181 SGVGDRQPKSKRRSLIRILKKAISKTISRNQKAVSSRVRESESYKHNEN-AAATQTQSSV 240

Query: 241 HLSSHASLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP 300
           HLSSHASLA EDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP
Sbjct: 241 HLSSHASLAPEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP 300

Query: 301 DYLLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEDDGTTHMKNQGNDHQST 360
           DYLLANLY AVLKELKGLIWNDKFDSTATSSSMNSSNSASIEE+DG TH+KN    HQST
Sbjct: 301 DYLLANLYAAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEEDGLTHLKN----HQST 360

Query: 361 Q---VANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLEFDGKLN 420
           +     N +VNLEA+S RKRERVYNTNYRTSQLMK+ADQNPK WKCEWERERLEFDGK+N
Sbjct: 361 ENFPAGNKMVNLEAESMRKRERVYNTNYRTSQLMKRADQNPKTWKCEWERERLEFDGKMN 420

Query: 421 NRHLD-HHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV 480
           N+ LD HHQIWDESTAINHSEVL+ALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV
Sbjct: 421 NKQLDHHHQIWDESTAINHSEVLNALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV 480

Query: 481 MLMRGEDVYLMNVGDSRAIVAQQLEPDFGIG-------RINEGTLRVFESSNGVEFERFT 540
           MLMRGEDVYLMNVGDSRAIVAQQLEPDFG G       RINEGTLRVFESSNGVEFER T
Sbjct: 481 MLMRGEDVYLMNVGDSRAIVAQQLEPDFGFGKQYRDLERINEGTLRVFESSNGVEFERLT 540

Query: 541 TLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGFLK------ 600
           TLASHQLTMDHSTYTEEEVQRIK AHP+D SAIMNDRVKGYLKITRAFGAGFLK      
Sbjct: 541 TLASHQLTMDHSTYTEEEVQRIKKAHPNDASAIMNDRVKGYLKITRAFGAGFLKQPKWND 600

Query: 601 -------------------------------------------QYFTNEEAVAHVESFIA 657
                                                      QYFTNEEAV+ VESFIA
Sbjct: 601 ALLEMFRIDYVGNSPYITCDPTLCHYKLSPTDRFLILSSDGLYQYFTNEEAVSQVESFIA 660

BLAST of Spg013529 vs. ExPASy Swiss-Prot
Match: Q9LQN6 (Probable protein phosphatase 2C 4 OS=Arabidopsis thaliana OX=3702 GN=PLL5 PE=2 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 2.9e-157
Identity = 350/729 (48.01%), Postives = 439/729 (60.22%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGDGDISRRYDPADYTA---DPNDGGFGHSFYYLPPDPHTFSSSKDFS 60
           MGN + K+  CF G G    R  P D +    DP D G GHSF Y+ PDP   SSSK  S
Sbjct: 1   MGNGVTKLSICFTGGG--GERLRPKDISVLLPDPLDEGLGHSFCYVRPDPTLISSSKVHS 60

Query: 61  EDYISPATIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPV 120
           E+  +  T FR+ISGASVSAN +TPLSTSL D Y +    DRAAAF+ + SF+S+PLQP+
Sbjct: 61  EE-DTTTTTFRTISGASVSANTATPLSTSLYDPYGH---IDRAAAFESTTSFSSIPLQPI 120

Query: 121 PRHSVSGNSGASGPIERGFLSGPLAARSFESGPIDR-GVYSGPIEKGGGP--EKLQRSFS 180
           P+ S     G SGPIERGFLSGP+  R F SGP+DR G++SGP++K       + QRSFS
Sbjct: 121 PKSSGPIVLG-SGPIERGFLSGPI-ERGFMSGPLDRVGLFSGPLDKPNSDHHHQFQRSFS 180

Query: 181 HGGVGDRQPKSKRRSLIRILKRAISKTISRNQKAVSSRVR------------ESESYKHN 240
           HG     +  S++RSL+RIL+RAISKT+SR Q ++ + ++            E     HN
Sbjct: 181 HGLA--LRVGSRKRSLVRILRRAISKTMSRGQNSIVAPIKSVKDSDNWGIRSEKSRNLHN 240

Query: 241 ENAAAAAQTQSSVHLSSHASLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVF 300
           EN      T +S++ SS  SL    D D  L  Q++QWAQGKAGEDRVHVV+SE++GW+F
Sbjct: 241 ENL-----TVNSLNFSSEVSL----DDDVSLENQNLQWAQGKAGEDRVHVVVSEEHGWLF 300

Query: 301 VGIYDGFNGPDAPDYLLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEDDGT 360
           VGIYDGFNGPDAPDYLL++LY  V +ELKGL+W+D         S   S S  +E  +G 
Sbjct: 301 VGIYDGFNGPDAPDYLLSHLYPVVHRELKGLLWDD---------SNVESKSQDLERSNGD 360

Query: 361 THMKNQGNDHQSTQVANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWER 420
               NQ                                      +K +   + W+CEW+R
Sbjct: 361 ESCSNQ--------------------------------------EKDETCERWWRCEWDR 420

Query: 421 ERLEFDGKLNNRHLDHHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPEL 480
           E  + D +L  + +      D  T  NHSEVL ALSQALRKTEEAYL  ADKM+  NPEL
Sbjct: 421 ESQDLDRRLKEQ-ISRRSGSDRLT--NHSEVLEALSQALRKTEEAYLDTADKMLDENPEL 480

Query: 481 ALMGSCVLVMLMRGEDVYLMNVGDSRAIVAQQLEPDFGIG-------RINEGT-LRVFES 540
           ALMGSCVLVMLM+GED+Y+MNVGDSRA++ Q+ EPD+ +        RINE T +   E 
Sbjct: 481 ALMGSCVLVMLMKGEDIYVMNVGDSRAVLGQKSEPDYWLAKIRQDLERINEETMMNDLEG 540

Query: 541 SNGVEFERFTTLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGA 600
             G +      L++ QLT+DHST  EEEV+RI+N HPDD +A+ N+RVKG LK+TRAFGA
Sbjct: 541 CEGDQSSLVPNLSAFQLTVDHSTNIEEEVERIRNEHPDDVTAVTNERVKGSLKVTRAFGA 600

Query: 601 GFLK-------------------------------------------------QYFTNEE 655
           GFLK                                                 QYFTNEE
Sbjct: 601 GFLKQPKWNNALLEMFQIDYVGKSPYINCLPSLYHHRLGSKDRFLILSSDGLYQYFTNEE 660

BLAST of Spg013529 vs. ExPASy Swiss-Prot
Match: Q9ZV25 (Probable protein phosphatase 2C 23 OS=Arabidopsis thaliana OX=3702 GN=PLL4 PE=2 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 2.1e-155
Identity = 341/718 (47.49%), Postives = 436/718 (60.72%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGDGDISRRYDPADYTADPNDGGFGHSFYYLPPDPHTFSSSKDFSEDY 60
           MGN IGK+  C  G    +++ + +    DP D G GHSF Y+ PDP   SSSK  SE+ 
Sbjct: 1   MGNGIGKLSKCLTGGAGRNKKPELSILEPDPLDEGLGHSFCYVRPDPTRVSSSKVHSEE- 60

Query: 61  ISPATIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPRH 120
               T FR+ISGASVSAN +TPLSTSL D Y +    DRAAAF+ + SF+S+PLQP+PR 
Sbjct: 61  --ETTTFRTISGASVSANTATPLSTSLYDPYGH---IDRAAAFESTTSFSSIPLQPIPRS 120

Query: 121 SVSGNSGASGPIERGFLSGPLAARSFESGPIDRGVYSGPIEKGGGPEKLQRSFSHGGVGD 180
           S     G SGP+ERGFLSGP+  R F SGP+D    SGPI+   G ++ QRSFSH G+ +
Sbjct: 121 SGPIVPG-SGPLERGFLSGPI-ERGFMSGPLDGS--SGPIDGKTGSDQFQRSFSH-GLAN 180

Query: 181 RQPKSKRRSLIRILKRAISKTISRNQKAVSSRVRESE-------SYKHNENAAAAAQTQS 240
            +  S++ SL+R+L+RAISKTI+R Q ++ + ++  +       S K   +      T +
Sbjct: 181 LRVGSRKGSLVRVLRRAISKTITRGQNSIVAPIKPVKEPDWVFGSDKTRIHQIENNLTVN 240

Query: 241 SVHLSSHASLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPD 300
           S++ SS  SL    D D  L  Q++QWAQGKAGEDRVHVV+SE++GW+FVGIYDGFNGPD
Sbjct: 241 SLNFSSEGSLL---DDDVSLESQNLQWAQGKAGEDRVHVVVSEEHGWLFVGIYDGFNGPD 300

Query: 301 APDYLLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEDDGTTHMKNQGNDHQ 360
           APDYLL++LY AV +ELKGL+W+D        +   SS+ A +E  D ++  K+      
Sbjct: 301 APDYLLSHLYPAVHRELKGLLWDD------PKTDAKSSDEADVENRDSSSEKKS------ 360

Query: 361 STQVANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLEFDGKLNN 420
                                            K  +++ + W+CEW+R+       L+ 
Sbjct: 361 ---------------------------------KNWEESQRRWRCEWDRD-------LDR 420

Query: 421 RHLDHHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLVML 480
              D     D     N S+VL ALSQALRKTEEAYL+NAD M+  NPELALMGSCVLVML
Sbjct: 421 LLKDRSNGLDLDPDPNSSDVLKALSQALRKTEEAYLENADMMLDENPELALMGSCVLVML 480

Query: 481 MRGEDVYLMNVGDSRAIVAQQLEPDFGIG-------RINEGTLRVFES-SNGVEFERFTT 540
           M+GEDVYLMNVGDSRA++ Q+ E D+ IG       RINE T+  F+   +G       T
Sbjct: 481 MKGEDVYLMNVGDSRAVLGQKAESDYWIGKIKQDLERINEETMNDFDGCGDGEGASLVPT 540

Query: 541 LASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGFLK------- 600
           L++ QLT+DHST  EEEV RI+  HPDD SA+ N+RVKG LK+TRAFGAGFLK       
Sbjct: 541 LSAFQLTVDHSTNVEEEVNRIRKEHPDDASAVSNERVKGSLKVTRAFGAGFLKQPKWNNA 600

Query: 601 ------------------------------------------QYFTNEEAVAHVESFIAS 655
                                                     QYFTNEEAV+ VE FI  
Sbjct: 601 LLEMFQIDYKGTSPYINCLPSLYHHRLGSKDQFLILSSDGLYQYFTNEEAVSEVELFITL 652

BLAST of Spg013529 vs. ExPASy Swiss-Prot
Match: Q9LZ86 (Probable protein phosphatase 2C 66 OS=Arabidopsis thaliana OX=3702 GN=PLL2 PE=2 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 2.7e-131
Identity = 328/729 (44.99%), Postives = 407/729 (55.83%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGD--GDISRRYDPADYTADPNDGGFGHSFYYLPPD-PHTF--SSSKD 60
           MGN +  +  C  G   G+ISRRYD           G GHSF Y+ PD P     S    
Sbjct: 1   MGNGVTTLTGCCTGTLAGEISRRYD-----VSLVHDGLGHSFCYIRPDLPGVVLPSPESP 60

Query: 61  FSEDYISPATIFRSISGASVSANVSTPLSTSLV--DLYPYSTTFDRAAAFDCSNSFASVP 120
              D+I   T FRSISGASVSAN ST LS +L      PYS+    A+AF+ S +FAS+P
Sbjct: 61  LRSDHIQETT-FRSISGASVSANPSTALSGALSSDSDCPYSSAVS-ASAFESSGNFASLP 120

Query: 121 LQPVPRHSVSGNSGASGPI--ERGFLSGPLAARSFESGPIDRGVYSGPIEKGGGPEKLQR 180
           LQPVPR    G++  SGPI  E G  S P   R F SGPI+ G+YSGPIE     EK + 
Sbjct: 121 LQPVPR----GSTWQSGPIVNESGLGSAPF-ERRFLSGPIESGLYSGPIESTKKTEKEKP 180

Query: 181 SFSHGGVGDRQPKSKRRSLIRILKRAISKTISRN-----QKAVSSRVRESESYKHNENAA 240
                    ++PKSK+  L    K   +  IS N     +K+V   +  S+S        
Sbjct: 181 KKIR-----KKPKSKKNFL--TFKTLFANLISNNNKPRLKKSVIEPINGSDSSDSGRLHH 240

Query: 241 AAAQTQSSVHLSSHASLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIY 300
               T S  + +  + L +ED+         VQWAQGKAGEDRVHVV+SEDNGWVFVGIY
Sbjct: 241 EPVITSSRSNENPKSDLEEEDEKQSMNSVLDVQWAQGKAGEDRVHVVVSEDNGWVFVGIY 300

Query: 301 DGFNGPDAPDYLLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEDDGTTHMK 360
           DGF+GPDAPDYLL NLYTAV KEL GL+WND+   +   + M  +   S +E+D  +  +
Sbjct: 301 DGFSGPDAPDYLLNNLYTAVQKELNGLLWNDEKLRSLGENGMTKTGKCS-DEEDPESGKE 360

Query: 361 NQGNDHQSTQVANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLE 420
           N    +    VA+G  N +A S +                         W+CEWE+    
Sbjct: 361 NCPVINNDDAVASGARN-QAKSLK-------------------------WRCEWEK---- 420

Query: 421 FDGKLNNRHLDHH---QIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELA 480
              K NN+    +   Q    ST  NH +VL AL QALRKTE+AYL+ AD+MV  NPELA
Sbjct: 421 ---KSNNKTKSDNRCDQKGSNSTTTNHKDVLKALLQALRKTEDAYLELADQMVKENPELA 480

Query: 481 LMGSCVLVMLMRGEDVYLMNVGDSRAIVAQQLEPDFGIGRINEGTLRVFESSNGVEFERF 540
           LMGSCVLV LM+GEDVY+MNVGDSRA++ +  +P+   GR  +  L      + +E +  
Sbjct: 481 LMGSCVLVTLMKGEDVYVMNVGDSRAVLGR--KPNLATGRKRQKELERIREDSSLEDKEI 540

Query: 541 -------TTLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGF 600
                   TL   QL M+HST  EEEV+RIK  HPDD  A+ NDRVKGYLK+TRAFGAGF
Sbjct: 541 LMNGAMRNTLVPLQLNMEHSTRIEEEVRRIKKEHPDDDCAVENDRVKGYLKVTRAFGAGF 600

Query: 601 LKQ-------------------------------------------------YFTNEEAV 657
           LKQ                                                 YF+N+EA+
Sbjct: 601 LKQPKWNDALLEMFRIDYIGTSPYITCSPSLCHHKLTSRDKFLILSSDGLYEYFSNQEAI 660

BLAST of Spg013529 vs. ExPASy Swiss-Prot
Match: Q84T94 (Protein phosphatase 2C 35 OS=Oryza sativa subsp. japonica OX=39947 GN=XB15 PE=1 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 4.6e-123
Identity = 314/729 (43.07%), Postives = 396/729 (54.32%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGDGDI----SRRYDPADYTADP-NDGGFGHSFYYLPPDPHTFSSSKD 60
           MGN +     CF   G       R   PA   +DP  D G GHSF Y+ PD      S D
Sbjct: 1   MGNSLA----CFCCGGGAGGRGGRHVAPAALPSDPAYDEGLGHSFCYVRPDKFVVPFSAD 60

Query: 61  FSEDYIS----------PATIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAA--F 120
              D ++           AT FR+ISGA++SANVSTPLSTS++ L P  ++    A+  F
Sbjct: 61  ---DLVADAKAAAAAEGEATTFRAISGAALSANVSTPLSTSVLLLMPEESSASATASSGF 120

Query: 121 DCSNSFASVPLQPVPRHSVSGNSGASGPIERGFLSGPLAARSFESGPIDRGVYSGPI--- 180
           + S SFA+VPLQPVPR S   +   S P   GF+SGPL  R F+SGP+D  + SGP+   
Sbjct: 121 ESSESFAAVPLQPVPRFS---SGPISAPFSGGFMSGPL-ERGFQSGPLDAALLSGPLPGT 180

Query: 181 ----EKGGGPEKLQRSFSHGGVGDRQPKSKRRSLIRILKRAISKTISRNQKAVSSRVRES 240
                 GG    L+RS SHGG   R+ ++  R+L+                A + + ++S
Sbjct: 181 ATSGRMGGAVPALRRSLSHGG---RRLRNFTRALL----------------ARTEKFQDS 240

Query: 241 ESYKHNENAAAAAQTQSSVHLSSHASLAQEDDGDYFLGGQ--SVQWAQGKAGEDRVHVVI 300
                 + AAAA                         GG    +QWAQGKAGEDRVHVV+
Sbjct: 241 ADLGSPDAAAAAVAA---------------------CGGDPCGLQWAQGKAGEDRVHVVV 300

Query: 301 SEDNGWVFVGIYDGFNGPDAPDYLLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSA 360
           SE+ GWVFVGIYDGFNGPDA D+L++NLY AV +EL+GL+W+ +  +       +   SA
Sbjct: 301 SEERGWVFVGIYDGFNGPDATDFLVSNLYAAVHRELRGLLWDQREQNVQHDQRPDQPGSA 360

Query: 361 SIEEDDGTTHMKNQGNDHQSTQVANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPK 420
                  TT   NQ                  D   +R R      R S+  + AD + +
Sbjct: 361 -----PSTTASDNQ------------------DQWGRRRRT-----RRSRPPRGADDDQR 420

Query: 421 NWKCEWERERLEFDGKLNNRHLDHHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADK 480
            WKCEWE+ER       +N      Q    ++  +H  VL AL++AL +TEEAYL  ADK
Sbjct: 421 RWKCEWEQER-----DCSNLKPPTQQRLRCNSENDHVAVLKALTRALHRTEEAYLDIADK 480

Query: 481 MVTRNPELALMGSCVLVMLMRGEDVYLMNVGDSRAIVAQQLEPDFGIGRINEGTLRVFES 540
           MV   PELALMGSCVL MLM+GED+Y+MNVGDSRA++A     D  + +I++G+   F+ 
Sbjct: 481 MVGEFPELALMGSCVLAMLMKGEDMYIMNVGDSRAVLATMDSVD--LEQISQGS---FDG 540

Query: 541 SNGVEFERFTTLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGA 600
           S G   +    L++ QLT DHST  EEEV RI+N HPDD SAI  DRVKG LK+TRAFGA
Sbjct: 541 SVG---DCPPCLSAVQLTSDHSTSVEEEVIRIRNEHPDDPSAISKDRVKGSLKVTRAFGA 600

Query: 601 GFLK-------------------------------------------------QYFTNEE 655
           GFLK                                                 QYFTNEE
Sbjct: 601 GFLKQPKWNDALLEMFRIDYVGSSPYISCNPSLFHHKLSTRDRFLILSSDGLYQYFTNEE 637

BLAST of Spg013529 vs. ExPASy Swiss-Prot
Match: Q9SR24 (Probable protein phosphatase 2C 36 OS=Arabidopsis thaliana OX=3702 GN=PLL3 PE=3 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 2.4e-119
Identity = 306/721 (42.44%), Postives = 392/721 (54.37%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGD--GDISRRYDPADYTADPNDGGFGHSFYYLPP----DPHTFSSSK 60
           MGN +     C AG   G+IS RY         N    GHSF Y+ P       +F    
Sbjct: 1   MGNGVASFSGCCAGTTAGEISGRYVTGVGLVQEN---LGHSFCYVRPVLTGSKSSFPPEP 60

Query: 61  DFSEDYI-SPATIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVP 120
               D I    T FRSISGASVSAN ST LSTSL      + T   A+AF+ SN FAS+P
Sbjct: 61  PLRPDPIPGTTTTFRSISGASVSANTSTALSTSL-----STDTSGIASAFESSNRFASLP 120

Query: 121 LQPVPRHSVSGNSGASGPIERGFLSGPLAARSFESGPIDRGVYSGPIEKGGGPEKLQRSF 180
           LQPVPR  +  +   SG  ER FL          SGPI+ G+ SG  +K     KL++S 
Sbjct: 121 LQPVPRSPIKKSDHGSGLFERRFL----------SGPIESGLVSG--KKTKEKAKLKKS- 180

Query: 181 SHGGVGDRQPKSKRRSLIRILKRAISKTISRNQKAVSSRVRESESYKHNENAAAAAQTQS 240
             G     +PK K+        + +   +S ++K+V   +   +S+  + +        +
Sbjct: 181 --GSKSFTKPKLKKSESKIFTFKNVFTNLSCSKKSVIKPINGFDSFDGSSDTDRYIPEIN 240

Query: 241 SVH--LSSHAS---LAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDG 300
           S+   +SSH       +ED  +  L    +QWAQGKAGEDRVHV++SE+NGW+FVGIYDG
Sbjct: 241 SLSTIVSSHEKPRIKEEEDKTESALEEPKIQWAQGKAGEDRVHVILSEENGWLFVGIYDG 300

Query: 301 FNGPDAPDYLLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEDDGTTHMKNQ 360
           F+GPD PDYL+ NLYTAVL+ELKGL+W DK        S N +  ++IE+     H  + 
Sbjct: 301 FSGPDPPDYLIKNLYTAVLRELKGLLWIDK------GESYNRNGESNIEKQSTVEHASD- 360

Query: 361 GNDHQSTQVANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLEFD 420
            +D ++  V NG  N  A  +R                  +D     W+CEWE       
Sbjct: 361 -SDQENCPVMNG--NDVACGSRN---------------ITSDVKKLQWRCEWE------- 420

Query: 421 GKLNNRHLDHHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELALMGSC 480
                     H   ++S  INH +VL AL QAL KTEE++    D MV  NPELALMGSC
Sbjct: 421 ----------HNSSNKSNNINHKDVLRALQQALEKTEESF----DLMVNENPELALMGSC 480

Query: 481 VLVMLMRGEDVYLMNVGDSRAIVAQQLEPDFGIGRINEGTLRVFESSN----GVEFERFT 540
           VLV LM+GEDVY+M+VGDSRA++A++  P+    ++ +   RV E S      +     +
Sbjct: 481 VLVTLMKGEDVYVMSVGDSRAVLARR--PNVEKMKMQKELERVKEESPLETLFITERGLS 540

Query: 541 TLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGFLKQ----- 600
            L   QL  +HST  EEEV+RIK  HPDD  AI N+RVKGYLK+TRAFGAGFLKQ     
Sbjct: 541 LLVPVQLNKEHSTSVEEEVRRIKKEHPDDILAIENNRVKGYLKVTRAFGAGFLKQPKWNE 600

Query: 601 --------------------------------------------YFTNEEAVAHVESFIA 657
                                                       YF+NEEA+  V+SFI+
Sbjct: 601 ALLEMFRIDYVGTSPYITCSPSLHHHRLSSRDKFLILSSDGLYEYFSNEEAIFEVDSFIS 650

BLAST of Spg013529 vs. ExPASy TrEMBL
Match: A0A6J1HRY6 (probable protein phosphatase 2C 23 OS=Cucurbita maxima OX=3661 GN=LOC111466201 PE=4 SV=1)

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 606/721 (84.05%), Postives = 628/721 (87.10%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGDGDISRRYDPADYTADPNDGGFGHSFYYLPPDPHTFSSSKDFSEDY 60
           MGNRIGKIG CFAGDGDISRRYDPAD   DP+DGGFGHSFYYLPPDPHT SSSKDFS++Y
Sbjct: 1   MGNRIGKIGRCFAGDGDISRRYDPADIIVDPHDGGFGHSFYYLPPDPHTISSSKDFSDEY 60

Query: 61  ISPA-TIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR 120
           ISPA TIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR
Sbjct: 61  ISPATTIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR 120

Query: 121 HSVSGNSGA---SGPIERGFLSGPLAARSFESGPIDRGVYSGPIEKGG-GPEKLQRSFSH 180
           HSVSGNSG    SGPIERGFLSGPLA+R+ ESGPIDRGV SGPIEK G GPEKL+RSFSH
Sbjct: 121 HSVSGNSGGFPYSGPIERGFLSGPLASRN-ESGPIDRGVCSGPIEKSGVGPEKLRRSFSH 180

Query: 181 GGVGDRQPKSKRRSLIRILKRAISKTISRNQKAVSSRVRESESYKHNENAAAAAQTQSSV 240
            GVGDRQPKSKRRSLIRILK+AISKTISRNQKAVSSRVRESESYKHNEN AAA QTQSSV
Sbjct: 181 SGVGDRQPKSKRRSLIRILKKAISKTISRNQKAVSSRVRESESYKHNEN-AAATQTQSSV 240

Query: 241 HLSSHASLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP 300
           HLSSHASLA EDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP
Sbjct: 241 HLSSHASLAPEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP 300

Query: 301 DYLLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEDDGTTHMKNQGNDHQST 360
           DYLLANLY AVL+ELKGLIW+DKFDSTATSSS+NSSNSASIEEDDGTTH+KN    HQST
Sbjct: 301 DYLLANLYAAVLRELKGLIWSDKFDSTATSSSVNSSNSASIEEDDGTTHLKN----HQST 360

Query: 361 Q---VANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLEFDGKLN 420
           +     N +VNLEA+S RKRERVYNTNYRTSQLMK+ADQNPK WKCEWERERLEFDGK+N
Sbjct: 361 ENFPAGNKMVNLEAESMRKRERVYNTNYRTSQLMKRADQNPKTWKCEWERERLEFDGKMN 420

Query: 421 NRHLD-HHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV 480
           N+ LD HHQIWDESTAINHSEVL+ALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV
Sbjct: 421 NKQLDHHHQIWDESTAINHSEVLNALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV 480

Query: 481 MLMRGEDVYLMNVGDSRAIVAQQLEPDFGIG-------RINEGTLRVFESSNGVEFERFT 540
           MLMRGEDVYLMNVGDSRAIVAQQLEPDFGIG       RINEGTLRVFESSNGVEFER T
Sbjct: 481 MLMRGEDVYLMNVGDSRAIVAQQLEPDFGIGKQYRDLERINEGTLRVFESSNGVEFERLT 540

Query: 541 TLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGFLK------ 600
           TLASHQLTMDHSTYTEEEVQRIK AHP+D SAIMNDRVKGYLKITRAFGAGFLK      
Sbjct: 541 TLASHQLTMDHSTYTEEEVQRIKKAHPNDASAIMNDRVKGYLKITRAFGAGFLKQPKWND 600

Query: 601 -------------------------------------------QYFTNEEAVAHVESFIA 657
                                                      QYFTNEEAV+ VESFIA
Sbjct: 601 ALLEMFRIDYVGNSPYITCDPTLCHYKLSPTDRFLILSSDGLYQYFTNEEAVSQVESFIA 660

BLAST of Spg013529 vs. ExPASy TrEMBL
Match: A0A6J1EZZ8 (probable protein phosphatase 2C 23 OS=Cucurbita moschata OX=3662 GN=LOC111438156 PE=4 SV=1)

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 605/721 (83.91%), Postives = 625/721 (86.69%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGDGDISRRYDPADYTADPNDGGFGHSFYYLPPDPHTFSSSKDFSEDY 60
           MGNRIGKIG CFAGDGDISRRYDPAD   DP+DGGFGHSFYYLPPDPHT SSSKDFS++Y
Sbjct: 1   MGNRIGKIGRCFAGDGDISRRYDPADIIVDPHDGGFGHSFYYLPPDPHTISSSKDFSDEY 60

Query: 61  ISPA-TIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR 120
           ISPA TIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR
Sbjct: 61  ISPATTIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPR 120

Query: 121 HSVSGNSGA---SGPIERGFLSGPLAARSFESGPIDRGVYSGPIEKGG-GPEKLQRSFSH 180
           HSVSGNSG    SGPIERGFLSGPLA+R+ ESGPIDRGV SGPIEK   GPEKL+RSFSH
Sbjct: 121 HSVSGNSGGFPYSGPIERGFLSGPLASRN-ESGPIDRGVCSGPIEKSAVGPEKLRRSFSH 180

Query: 181 GGVGDRQPKSKRRSLIRILKRAISKTISRNQKAVSSRVRESESYKHNENAAAAAQTQSSV 240
            GVGDRQPKSKRRSLIRILK+AISKTISRNQKAVSSRVRESESYKHNEN AAA QTQSSV
Sbjct: 181 SGVGDRQPKSKRRSLIRILKKAISKTISRNQKAVSSRVRESESYKHNEN-AAATQTQSSV 240

Query: 241 HLSSHASLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP 300
           HLSSHASLA EDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP
Sbjct: 241 HLSSHASLAPEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAP 300

Query: 301 DYLLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEDDGTTHMKNQGNDHQST 360
           DYLLANLY AVLKELKGLIWNDKFDSTATSSSMNSSNSASIEE+DG TH+KN    HQST
Sbjct: 301 DYLLANLYAAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEEDGLTHLKN----HQST 360

Query: 361 Q---VANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLEFDGKLN 420
           +     N +VNLEA+S RKRERVYNTNYRTSQLMK+ADQNPK WKCEWERERLEFDGK+N
Sbjct: 361 ENFPAGNKMVNLEAESMRKRERVYNTNYRTSQLMKRADQNPKTWKCEWERERLEFDGKMN 420

Query: 421 NRHLD-HHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV 480
           N+ LD HHQIWDESTAINHSEVL+ALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV
Sbjct: 421 NKQLDHHHQIWDESTAINHSEVLNALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLV 480

Query: 481 MLMRGEDVYLMNVGDSRAIVAQQLEPDFGIG-------RINEGTLRVFESSNGVEFERFT 540
           MLMRGEDVYLMNVGDSRAIVAQQLEPDFG G       RINEGTLRVFESSNGVEFER T
Sbjct: 481 MLMRGEDVYLMNVGDSRAIVAQQLEPDFGFGKQYRDLERINEGTLRVFESSNGVEFERLT 540

Query: 541 TLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGFLK------ 600
           TLASHQLTMDHSTYTEEEVQRIK AHP+D SAIMNDRVKGYLKITRAFGAGFLK      
Sbjct: 541 TLASHQLTMDHSTYTEEEVQRIKKAHPNDASAIMNDRVKGYLKITRAFGAGFLKQPKWND 600

Query: 601 -------------------------------------------QYFTNEEAVAHVESFIA 657
                                                      QYFTNEEAV+ VESFIA
Sbjct: 601 ALLEMFRIDYVGNSPYITCDPTLCHYKLSPTDRFLILSSDGLYQYFTNEEAVSQVESFIA 660

BLAST of Spg013529 vs. ExPASy TrEMBL
Match: A0A5A7SN99 (PPM-type phosphatase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G00380 PE=4 SV=1)

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 589/727 (81.02%), Postives = 608/727 (83.63%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGDGDISRRYDPADYTADPNDGGFGHSFYYLPPDPHTFSSSKDFSEDY 60
           MGNRIGKIGHCFAG GDISRRYDPAD  +DP DGGFGHSFYYLPPDPHT SSSKDFS+DY
Sbjct: 1   MGNRIGKIGHCFAGAGDISRRYDPADIISDPQDGGFGHSFYYLPPDPHTLSSSKDFSDDY 60

Query: 61  ISPATIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPRH 120
           ISP T FRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDC+NSFASVPLQPVPRH
Sbjct: 61  ISPVTFFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCTNSFASVPLQPVPRH 120

Query: 121 SVSGNSG---ASGPIERGFLSGPLAARSFESGPIDRGVYSGPIEKGGGPEKLQRSFSHGG 180
           SVSGNSG   +SGP+ERGFLSGPLAARSFESGPIDR V+SGPIEK GGPEKLQRS SHG 
Sbjct: 121 SVSGNSGGFPSSGPLERGFLSGPLAARSFESGPIDRVVHSGPIEKDGGPEKLQRSVSHGR 180

Query: 181 VGDRQPKSK-----RRSLIRILKRAISKTISRNQKAVSSRVRESESYKHNEN-AAAAAQT 240
           VGDR+PK K     RR+LIRILKRAISKTISRN              KHNEN AA+A QT
Sbjct: 181 VGDREPKLKKRRRRRRNLIRILKRAISKTISRN--------------KHNENGAASAGQT 240

Query: 241 QSSVHLSSHASLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNG 300
           QSSVHLSSH SLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNG
Sbjct: 241 QSSVHLSSHGSLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNG 300

Query: 301 PDAPDYLLANLYTAVLKELK-GLIWNDKFDSTATSSSMNSSNSASIEEDD-GTTHMKNQG 360
           PDAPDYLLANLYTAVLKELK  LIW+DKFDSTATSSSMNSSNSAS EEDD  TTHMKNQ 
Sbjct: 301 PDAPDYLLANLYTAVLKELKSSLIWSDKFDSTATSSSMNSSNSASTEEDDHRTTHMKNQE 360

Query: 361 NDHQSTQ---VANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLE 420
           NDHQST+     N +VNLEAD  RKRERV+NTNYRTSQLMKKADQNPK WKCEWERERL 
Sbjct: 361 NDHQSTENILPENEVVNLEADLVRKRERVHNTNYRTSQLMKKADQNPKYWKCEWERERLG 420

Query: 421 FDGKL-NNRHLDHHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELALM 480
           F+GKL NNR +DHHQIWDESTAINHSEVL+ALSQALRKTEEAYLQNADKMVTRNPELALM
Sbjct: 421 FEGKLNNNRQIDHHQIWDESTAINHSEVLNALSQALRKTEEAYLQNADKMVTRNPELALM 480

Query: 481 GSCVLVMLMRGEDVYLMNVGDSRAIVAQQLEPDFGIG-------RINEGTLRVFESSNGV 540
           GSCVLVMLMRGEDVYLMNVGDSRAIVAQQ EPDFG G       RINEGT+RVFESSNGV
Sbjct: 481 GSCVLVMLMRGEDVYLMNVGDSRAIVAQQFEPDFGTGKPYRDLERINEGTIRVFESSNGV 540

Query: 541 EFERFTTLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGFLK 600
           EFE+   LASHQLTMDHSTY EEEVQRIKNAHPDD SAIMNDRVKGYLKITRAFGAGFLK
Sbjct: 541 EFEKLKALASHQLTMDHSTYIEEEVQRIKNAHPDDASAIMNDRVKGYLKITRAFGAGFLK 600

Query: 601 -------------------------------------------------QYFTNEEAVAH 657
                                                            QYFTNEEAVA 
Sbjct: 601 QPKWNDALLEMFRIDYVGNSPYITCDPTLCHYKLSPTDRFLILSSDGLYQYFTNEEAVAQ 660

BLAST of Spg013529 vs. ExPASy TrEMBL
Match: A0A1S3BI01 (probable protein phosphatase 2C 4 OS=Cucumis melo OX=3656 GN=LOC103490267 PE=4 SV=1)

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 589/727 (81.02%), Postives = 608/727 (83.63%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGDGDISRRYDPADYTADPNDGGFGHSFYYLPPDPHTFSSSKDFSEDY 60
           MGNRIGKIGHCFAG GDISRRYDPAD  +DP DGGFGHSFYYLPPDPHT SSSKDFS+DY
Sbjct: 1   MGNRIGKIGHCFAGAGDISRRYDPADIISDPQDGGFGHSFYYLPPDPHTLSSSKDFSDDY 60

Query: 61  ISPATIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPRH 120
           ISP T FRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDC+NSFASVPLQPVPRH
Sbjct: 61  ISPVTFFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCTNSFASVPLQPVPRH 120

Query: 121 SVSGNSG---ASGPIERGFLSGPLAARSFESGPIDRGVYSGPIEKGGGPEKLQRSFSHGG 180
           SVSGNSG   +SGP+ERGFLSGPLAARSFESGPIDR V+SGPIEK GGPEKLQRS SHG 
Sbjct: 121 SVSGNSGGFPSSGPLERGFLSGPLAARSFESGPIDRVVHSGPIEKDGGPEKLQRSVSHGR 180

Query: 181 VGDRQPKSK-----RRSLIRILKRAISKTISRNQKAVSSRVRESESYKHNEN-AAAAAQT 240
           VGDR+PK K     RR+LIRILKRAISKTISRN              KHNEN AA+A QT
Sbjct: 181 VGDREPKLKKRRRRRRNLIRILKRAISKTISRN--------------KHNENGAASAGQT 240

Query: 241 QSSVHLSSHASLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNG 300
           QSSVHLSSH SLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNG
Sbjct: 241 QSSVHLSSHGSLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNG 300

Query: 301 PDAPDYLLANLYTAVLKELK-GLIWNDKFDSTATSSSMNSSNSASIEEDD-GTTHMKNQG 360
           PDAPDYLLANLYTAVLKELK  LIW+DKFDSTATSSSMNSSNSAS EEDD  TTHMKNQ 
Sbjct: 301 PDAPDYLLANLYTAVLKELKSSLIWSDKFDSTATSSSMNSSNSASTEEDDHRTTHMKNQE 360

Query: 361 NDHQSTQ---VANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLE 420
           NDHQST+     N +VNLEAD  RKRERV+NTNYRTSQLMKKADQNPK WKCEWERERL 
Sbjct: 361 NDHQSTENILPENEVVNLEADLVRKRERVHNTNYRTSQLMKKADQNPKYWKCEWERERLG 420

Query: 421 FDGKL-NNRHLDHHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELALM 480
           F+GKL NNR +DHHQIWDESTAINHSEVL+ALSQALRKTEEAYLQNADKMVTRNPELALM
Sbjct: 421 FEGKLNNNRQIDHHQIWDESTAINHSEVLNALSQALRKTEEAYLQNADKMVTRNPELALM 480

Query: 481 GSCVLVMLMRGEDVYLMNVGDSRAIVAQQLEPDFGIG-------RINEGTLRVFESSNGV 540
           GSCVLVMLMRGEDVYLMNVGDSRAIVAQQ EPDFG G       RINEGT+RVFESSNGV
Sbjct: 481 GSCVLVMLMRGEDVYLMNVGDSRAIVAQQFEPDFGTGKPYRDLERINEGTIRVFESSNGV 540

Query: 541 EFERFTTLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGFLK 600
           EFE+   LASHQLTMDHSTY EEEVQRIKNAHPDD SAIMNDRVKGYLKITRAFGAGFLK
Sbjct: 541 EFEKLKALASHQLTMDHSTYIEEEVQRIKNAHPDDASAIMNDRVKGYLKITRAFGAGFLK 600

Query: 601 -------------------------------------------------QYFTNEEAVAH 657
                                                            QYFTNEEAVA 
Sbjct: 601 QPKWNDALLEMFRIDYVGNSPYITCDPTLCHYKLSPTDRFLILSSDGLYQYFTNEEAVAQ 660

BLAST of Spg013529 vs. ExPASy TrEMBL
Match: A0A6J1ISC5 (probable protein phosphatase 2C 23 OS=Cucurbita maxima OX=3661 GN=LOC111478861 PE=4 SV=1)

HSP 1 Score: 975.3 bits (2520), Expect = 1.2e-280
Identity = 542/709 (76.45%), Postives = 563/709 (79.41%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGDGDISRRYDPADYTADPNDGGFGHSFYYLPPDPHTFSSSKDFSEDY 60
           MGN IGKIGHCFAG GDIS RY PAD  +DP+DGGFGHSFYYLPPDP T SS K F EDY
Sbjct: 1   MGNHIGKIGHCFAGAGDISSRYGPADIISDPHDGGFGHSFYYLPPDPQTLSSFKAFPEDY 60

Query: 61  ISPATIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPRH 120
           ISPATIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPRH
Sbjct: 61  ISPATIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPRH 120

Query: 121 SVSGNSG---ASGPIERGFLSGPLAARSFESGPIDRGVYSGPIEKGGGPEKLQRSFSHGG 180
           S+SGN G   +SGP+ERGFLSGPLAARS ES PIDR VYSG IEKGGGPEKLQRS S+GG
Sbjct: 121 SMSGNFGGNPSSGPMERGFLSGPLAARSIESCPIDRVVYSGSIEKGGGPEKLQRSVSNGG 180

Query: 181 VGDRQPKSKRRSLIRILKRAISKTISRNQKAVSSRVRESESYKHNENAAAAAQTQSSVHL 240
           VGDRQPKSKRRSLIRILKRAISKTISRNQKA S       SYKH EN   AAQTQSSVHL
Sbjct: 181 VGDRQPKSKRRSLIRILKRAISKTISRNQKAAS-------SYKH-EN---AAQTQSSVHL 240

Query: 241 SSHASLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPDAPDY 300
           SSHASLAQEDDGDYF+GGQ VQWAQGKAGEDRVHVVI E+NGWVFVG+YDGFNGPDAPDY
Sbjct: 241 SSHASLAQEDDGDYFVGGQGVQWAQGKAGEDRVHVVILEENGWVFVGVYDGFNGPDAPDY 300

Query: 301 LLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEDD-GTTHMKNQGNDHQSTQ 360
           LLANLYTAVLKELKGLIWNDK DSTAT+SSMNSSNSA IEEDD GTTH+K Q N HQ T 
Sbjct: 301 LLANLYTAVLKELKGLIWNDKLDSTATTSSMNSSNSALIEEDDHGTTHIKTQANVHQPT- 360

Query: 361 VANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLEFDGKLNNRHL 420
                VN  A++            RT QLMKK DQ+P+NWK  WERER        +  L
Sbjct: 361 -----VNFSAEN------------RTRQLMKKGDQDPENWK--WERER-------ESIGL 420

Query: 421 DHHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLVMLMRG 480
           DHH IWDEST INHSEVL+ALS+AL+KTEE+YLQNADKM TRNPELALMGSCVLVMLMRG
Sbjct: 421 DHHNIWDESTTINHSEVLNALSEALKKTEESYLQNADKMATRNPELALMGSCVLVMLMRG 480

Query: 481 EDVYLMNVGDSRAIVAQQLEPDFGIGRINEGTLRVFESSNGVEFERFTTLASHQLTMDHS 540
           EDVYLMNVGDSRAIVAQQLEP   + RINEGTLRV ES      ER T LASHQLTMDHS
Sbjct: 481 EDVYLMNVGDSRAIVAQQLEPYRDLERINEGTLRVLESP-----ERLTALASHQLTMDHS 540

Query: 541 TYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGFLK------------------ 600
           TYTEEEVQRIKNAH DD SAIMNDRVKGYLKITRAFGAGFLK                  
Sbjct: 541 TYTEEEVQRIKNAHSDDPSAIMNDRVKGYLKITRAFGAGFLKQPKWNDALLEMFRIDYVG 600

Query: 601 -------------------------------QYFTNEEAVAHVESFIASFPEGDPAQNLI 657
                                          QYFTNEEAVA VESFIASFPEGDPAQNLI
Sbjct: 601 NSPYITCDPTLCHYKLSPTDRFLILSSDGLYQYFTNEEAVAQVESFIASFPEGDPAQNLI 660

BLAST of Spg013529 vs. TAIR 10
Match: AT1G07630.1 (pol-like 5 )

HSP 1 Score: 557.0 bits (1434), Expect = 2.0e-158
Identity = 350/729 (48.01%), Postives = 439/729 (60.22%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGDGDISRRYDPADYTA---DPNDGGFGHSFYYLPPDPHTFSSSKDFS 60
           MGN + K+  CF G G    R  P D +    DP D G GHSF Y+ PDP   SSSK  S
Sbjct: 1   MGNGVTKLSICFTGGG--GERLRPKDISVLLPDPLDEGLGHSFCYVRPDPTLISSSKVHS 60

Query: 61  EDYISPATIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPV 120
           E+  +  T FR+ISGASVSAN +TPLSTSL D Y +    DRAAAF+ + SF+S+PLQP+
Sbjct: 61  EE-DTTTTTFRTISGASVSANTATPLSTSLYDPYGH---IDRAAAFESTTSFSSIPLQPI 120

Query: 121 PRHSVSGNSGASGPIERGFLSGPLAARSFESGPIDR-GVYSGPIEKGGGP--EKLQRSFS 180
           P+ S     G SGPIERGFLSGP+  R F SGP+DR G++SGP++K       + QRSFS
Sbjct: 121 PKSSGPIVLG-SGPIERGFLSGPI-ERGFMSGPLDRVGLFSGPLDKPNSDHHHQFQRSFS 180

Query: 181 HGGVGDRQPKSKRRSLIRILKRAISKTISRNQKAVSSRVR------------ESESYKHN 240
           HG     +  S++RSL+RIL+RAISKT+SR Q ++ + ++            E     HN
Sbjct: 181 HGLA--LRVGSRKRSLVRILRRAISKTMSRGQNSIVAPIKSVKDSDNWGIRSEKSRNLHN 240

Query: 241 ENAAAAAQTQSSVHLSSHASLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVF 300
           EN      T +S++ SS  SL    D D  L  Q++QWAQGKAGEDRVHVV+SE++GW+F
Sbjct: 241 ENL-----TVNSLNFSSEVSL----DDDVSLENQNLQWAQGKAGEDRVHVVVSEEHGWLF 300

Query: 301 VGIYDGFNGPDAPDYLLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEDDGT 360
           VGIYDGFNGPDAPDYLL++LY  V +ELKGL+W+D         S   S S  +E  +G 
Sbjct: 301 VGIYDGFNGPDAPDYLLSHLYPVVHRELKGLLWDD---------SNVESKSQDLERSNGD 360

Query: 361 THMKNQGNDHQSTQVANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWER 420
               NQ                                      +K +   + W+CEW+R
Sbjct: 361 ESCSNQ--------------------------------------EKDETCERWWRCEWDR 420

Query: 421 ERLEFDGKLNNRHLDHHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPEL 480
           E  + D +L  + +      D  T  NHSEVL ALSQALRKTEEAYL  ADKM+  NPEL
Sbjct: 421 ESQDLDRRLKEQ-ISRRSGSDRLT--NHSEVLEALSQALRKTEEAYLDTADKMLDENPEL 480

Query: 481 ALMGSCVLVMLMRGEDVYLMNVGDSRAIVAQQLEPDFGIG-------RINEGT-LRVFES 540
           ALMGSCVLVMLM+GED+Y+MNVGDSRA++ Q+ EPD+ +        RINE T +   E 
Sbjct: 481 ALMGSCVLVMLMKGEDIYVMNVGDSRAVLGQKSEPDYWLAKIRQDLERINEETMMNDLEG 540

Query: 541 SNGVEFERFTTLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGA 600
             G +      L++ QLT+DHST  EEEV+RI+N HPDD +A+ N+RVKG LK+TRAFGA
Sbjct: 541 CEGDQSSLVPNLSAFQLTVDHSTNIEEEVERIRNEHPDDVTAVTNERVKGSLKVTRAFGA 600

Query: 601 GFLK-------------------------------------------------QYFTNEE 655
           GFLK                                                 QYFTNEE
Sbjct: 601 GFLKQPKWNNALLEMFQIDYVGKSPYINCLPSLYHHRLGSKDRFLILSSDGLYQYFTNEE 660

BLAST of Spg013529 vs. TAIR 10
Match: AT2G28890.1 (poltergeist like 4 )

HSP 1 Score: 550.8 bits (1418), Expect = 1.5e-156
Identity = 341/718 (47.49%), Postives = 436/718 (60.72%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGDGDISRRYDPADYTADPNDGGFGHSFYYLPPDPHTFSSSKDFSEDY 60
           MGN IGK+  C  G    +++ + +    DP D G GHSF Y+ PDP   SSSK  SE+ 
Sbjct: 1   MGNGIGKLSKCLTGGAGRNKKPELSILEPDPLDEGLGHSFCYVRPDPTRVSSSKVHSEE- 60

Query: 61  ISPATIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVPLQPVPRH 120
               T FR+ISGASVSAN +TPLSTSL D Y +    DRAAAF+ + SF+S+PLQP+PR 
Sbjct: 61  --ETTTFRTISGASVSANTATPLSTSLYDPYGH---IDRAAAFESTTSFSSIPLQPIPRS 120

Query: 121 SVSGNSGASGPIERGFLSGPLAARSFESGPIDRGVYSGPIEKGGGPEKLQRSFSHGGVGD 180
           S     G SGP+ERGFLSGP+  R F SGP+D    SGPI+   G ++ QRSFSH G+ +
Sbjct: 121 SGPIVPG-SGPLERGFLSGPI-ERGFMSGPLDGS--SGPIDGKTGSDQFQRSFSH-GLAN 180

Query: 181 RQPKSKRRSLIRILKRAISKTISRNQKAVSSRVRESE-------SYKHNENAAAAAQTQS 240
            +  S++ SL+R+L+RAISKTI+R Q ++ + ++  +       S K   +      T +
Sbjct: 181 LRVGSRKGSLVRVLRRAISKTITRGQNSIVAPIKPVKEPDWVFGSDKTRIHQIENNLTVN 240

Query: 241 SVHLSSHASLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDGFNGPD 300
           S++ SS  SL    D D  L  Q++QWAQGKAGEDRVHVV+SE++GW+FVGIYDGFNGPD
Sbjct: 241 SLNFSSEGSLL---DDDVSLESQNLQWAQGKAGEDRVHVVVSEEHGWLFVGIYDGFNGPD 300

Query: 301 APDYLLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEDDGTTHMKNQGNDHQ 360
           APDYLL++LY AV +ELKGL+W+D        +   SS+ A +E  D ++  K+      
Sbjct: 301 APDYLLSHLYPAVHRELKGLLWDD------PKTDAKSSDEADVENRDSSSEKKS------ 360

Query: 361 STQVANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLEFDGKLNN 420
                                            K  +++ + W+CEW+R+       L+ 
Sbjct: 361 ---------------------------------KNWEESQRRWRCEWDRD-------LDR 420

Query: 421 RHLDHHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELALMGSCVLVML 480
              D     D     N S+VL ALSQALRKTEEAYL+NAD M+  NPELALMGSCVLVML
Sbjct: 421 LLKDRSNGLDLDPDPNSSDVLKALSQALRKTEEAYLENADMMLDENPELALMGSCVLVML 480

Query: 481 MRGEDVYLMNVGDSRAIVAQQLEPDFGIG-------RINEGTLRVFES-SNGVEFERFTT 540
           M+GEDVYLMNVGDSRA++ Q+ E D+ IG       RINE T+  F+   +G       T
Sbjct: 481 MKGEDVYLMNVGDSRAVLGQKAESDYWIGKIKQDLERINEETMNDFDGCGDGEGASLVPT 540

Query: 541 LASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGFLK------- 600
           L++ QLT+DHST  EEEV RI+  HPDD SA+ N+RVKG LK+TRAFGAGFLK       
Sbjct: 541 LSAFQLTVDHSTNVEEEVNRIRKEHPDDASAVSNERVKGSLKVTRAFGAGFLKQPKWNNA 600

Query: 601 ------------------------------------------QYFTNEEAVAHVESFIAS 655
                                                     QYFTNEEAV+ VE FI  
Sbjct: 601 LLEMFQIDYKGTSPYINCLPSLYHHRLGSKDQFLILSSDGLYQYFTNEEAVSEVELFITL 652

BLAST of Spg013529 vs. TAIR 10
Match: AT5G02400.1 (pol-like 2 )

HSP 1 Score: 470.7 bits (1210), Expect = 1.9e-132
Identity = 328/729 (44.99%), Postives = 407/729 (55.83%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGD--GDISRRYDPADYTADPNDGGFGHSFYYLPPD-PHTF--SSSKD 60
           MGN +  +  C  G   G+ISRRYD           G GHSF Y+ PD P     S    
Sbjct: 1   MGNGVTTLTGCCTGTLAGEISRRYD-----VSLVHDGLGHSFCYIRPDLPGVVLPSPESP 60

Query: 61  FSEDYISPATIFRSISGASVSANVSTPLSTSLV--DLYPYSTTFDRAAAFDCSNSFASVP 120
              D+I   T FRSISGASVSAN ST LS +L      PYS+    A+AF+ S +FAS+P
Sbjct: 61  LRSDHIQETT-FRSISGASVSANPSTALSGALSSDSDCPYSSAVS-ASAFESSGNFASLP 120

Query: 121 LQPVPRHSVSGNSGASGPI--ERGFLSGPLAARSFESGPIDRGVYSGPIEKGGGPEKLQR 180
           LQPVPR    G++  SGPI  E G  S P   R F SGPI+ G+YSGPIE     EK + 
Sbjct: 121 LQPVPR----GSTWQSGPIVNESGLGSAPF-ERRFLSGPIESGLYSGPIESTKKTEKEKP 180

Query: 181 SFSHGGVGDRQPKSKRRSLIRILKRAISKTISRN-----QKAVSSRVRESESYKHNENAA 240
                    ++PKSK+  L    K   +  IS N     +K+V   +  S+S        
Sbjct: 181 KKIR-----KKPKSKKNFL--TFKTLFANLISNNNKPRLKKSVIEPINGSDSSDSGRLHH 240

Query: 241 AAAQTQSSVHLSSHASLAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIY 300
               T S  + +  + L +ED+         VQWAQGKAGEDRVHVV+SEDNGWVFVGIY
Sbjct: 241 EPVITSSRSNENPKSDLEEEDEKQSMNSVLDVQWAQGKAGEDRVHVVVSEDNGWVFVGIY 300

Query: 301 DGFNGPDAPDYLLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEDDGTTHMK 360
           DGF+GPDAPDYLL NLYTAV KEL GL+WND+   +   + M  +   S +E+D  +  +
Sbjct: 301 DGFSGPDAPDYLLNNLYTAVQKELNGLLWNDEKLRSLGENGMTKTGKCS-DEEDPESGKE 360

Query: 361 NQGNDHQSTQVANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLE 420
           N    +    VA+G  N +A S +                         W+CEWE+    
Sbjct: 361 NCPVINNDDAVASGARN-QAKSLK-------------------------WRCEWEK---- 420

Query: 421 FDGKLNNRHLDHH---QIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELA 480
              K NN+    +   Q    ST  NH +VL AL QALRKTE+AYL+ AD+MV  NPELA
Sbjct: 421 ---KSNNKTKSDNRCDQKGSNSTTTNHKDVLKALLQALRKTEDAYLELADQMVKENPELA 480

Query: 481 LMGSCVLVMLMRGEDVYLMNVGDSRAIVAQQLEPDFGIGRINEGTLRVFESSNGVEFERF 540
           LMGSCVLV LM+GEDVY+MNVGDSRA++ +  +P+   GR  +  L      + +E +  
Sbjct: 481 LMGSCVLVTLMKGEDVYVMNVGDSRAVLGR--KPNLATGRKRQKELERIREDSSLEDKEI 540

Query: 541 -------TTLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGF 600
                   TL   QL M+HST  EEEV+RIK  HPDD  A+ NDRVKGYLK+TRAFGAGF
Sbjct: 541 LMNGAMRNTLVPLQLNMEHSTRIEEEVRRIKKEHPDDDCAVENDRVKGYLKVTRAFGAGF 600

Query: 601 LKQ-------------------------------------------------YFTNEEAV 657
           LKQ                                                 YF+N+EA+
Sbjct: 601 LKQPKWNDALLEMFRIDYIGTSPYITCSPSLCHHKLTSRDKFLILSSDGLYEYFSNQEAI 660

BLAST of Spg013529 vs. TAIR 10
Match: AT3G09400.1 (pol-like 3 )

HSP 1 Score: 431.0 bits (1107), Expect = 1.7e-120
Identity = 306/721 (42.44%), Postives = 392/721 (54.37%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGD--GDISRRYDPADYTADPNDGGFGHSFYYLPP----DPHTFSSSK 60
           MGN +     C AG   G+IS RY         N    GHSF Y+ P       +F    
Sbjct: 1   MGNGVASFSGCCAGTTAGEISGRYVTGVGLVQEN---LGHSFCYVRPVLTGSKSSFPPEP 60

Query: 61  DFSEDYI-SPATIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVP 120
               D I    T FRSISGASVSAN ST LSTSL      + T   A+AF+ SN FAS+P
Sbjct: 61  PLRPDPIPGTTTTFRSISGASVSANTSTALSTSL-----STDTSGIASAFESSNRFASLP 120

Query: 121 LQPVPRHSVSGNSGASGPIERGFLSGPLAARSFESGPIDRGVYSGPIEKGGGPEKLQRSF 180
           LQPVPR  +  +   SG  ER FL          SGPI+ G+ SG  +K     KL++S 
Sbjct: 121 LQPVPRSPIKKSDHGSGLFERRFL----------SGPIESGLVSG--KKTKEKAKLKKS- 180

Query: 181 SHGGVGDRQPKSKRRSLIRILKRAISKTISRNQKAVSSRVRESESYKHNENAAAAAQTQS 240
             G     +PK K+        + +   +S ++K+V   +   +S+  + +        +
Sbjct: 181 --GSKSFTKPKLKKSESKIFTFKNVFTNLSCSKKSVIKPINGFDSFDGSSDTDRYIPEIN 240

Query: 241 SVH--LSSHAS---LAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDG 300
           S+   +SSH       +ED  +  L    +QWAQGKAGEDRVHV++SE+NGW+FVGIYDG
Sbjct: 241 SLSTIVSSHEKPRIKEEEDKTESALEEPKIQWAQGKAGEDRVHVILSEENGWLFVGIYDG 300

Query: 301 FNGPDAPDYLLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEDDGTTHMKNQ 360
           F+GPD PDYL+ NLYTAVL+ELKGL+W DK        S N +  ++IE+     H  + 
Sbjct: 301 FSGPDPPDYLIKNLYTAVLRELKGLLWIDK------GESYNRNGESNIEKQSTVEHASD- 360

Query: 361 GNDHQSTQVANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLEFD 420
            +D ++  V NG  N  A  +R                  +D     W+CEWE       
Sbjct: 361 -SDQENCPVMNG--NDVACGSRN---------------ITSDVKKLQWRCEWE------- 420

Query: 421 GKLNNRHLDHHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELALMGSC 480
                     H   ++S  INH +VL AL QAL KTEE++    D MV  NPELALMGSC
Sbjct: 421 ----------HNSSNKSNNINHKDVLRALQQALEKTEESF----DLMVNENPELALMGSC 480

Query: 481 VLVMLMRGEDVYLMNVGDSRAIVAQQLEPDFGIGRINEGTLRVFESSN----GVEFERFT 540
           VLV LM+GEDVY+M+VGDSRA++A++  P+    ++ +   RV E S      +     +
Sbjct: 481 VLVTLMKGEDVYVMSVGDSRAVLARR--PNVEKMKMQKELERVKEESPLETLFITERGLS 540

Query: 541 TLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGFLKQ----- 600
            L   QL  +HST  EEEV+RIK  HPDD  AI N+RVKGYLK+TRAFGAGFLKQ     
Sbjct: 541 LLVPVQLNKEHSTSVEEEVRRIKKEHPDDILAIENNRVKGYLKVTRAFGAGFLKQPKWNE 600

Query: 601 --------------------------------------------YFTNEEAVAHVESFIA 657
                                                       YF+NEEA+  V+SFI+
Sbjct: 601 ALLEMFRIDYVGTSPYITCSPSLHHHRLSSRDKFLILSSDGLYEYFSNEEAIFEVDSFIS 650

BLAST of Spg013529 vs. TAIR 10
Match: AT3G09400.2 (pol-like 3 )

HSP 1 Score: 365.5 bits (937), Expect = 8.7e-101
Identity = 276/684 (40.35%), Postives = 358/684 (52.34%), Query Frame = 0

Query: 1   MGNRIGKIGHCFAGD--GDISRRYDPADYTADPNDGGFGHSFYYLPP----DPHTFSSSK 60
           MGN +     C AG   G+IS RY         N    GHSF Y+ P       +F    
Sbjct: 1   MGNGVASFSGCCAGTTAGEISGRYVTGVGLVQEN---LGHSFCYVRPVLTGSKSSFPPEP 60

Query: 61  DFSEDYI-SPATIFRSISGASVSANVSTPLSTSLVDLYPYSTTFDRAAAFDCSNSFASVP 120
               D I    T FRSISGASVSAN ST LSTSL      + T   A+AF+ SN FAS+P
Sbjct: 61  PLRPDPIPGTTTTFRSISGASVSANTSTALSTSL-----STDTSGIASAFESSNRFASLP 120

Query: 121 LQPVPRHSVSGNSGASGPIERGFLSGPLAARSFESGPIDRGVYSGPIEKGGGPEKLQRSF 180
           LQPVPR  +  +   SG  ER FL          SGPI+ G+ SG  +K     KL++S 
Sbjct: 121 LQPVPRSPIKKSDHGSGLFERRFL----------SGPIESGLVSG--KKTKEKAKLKKS- 180

Query: 181 SHGGVGDRQPKSKRRSLIRILKRAISKTISRNQKAVSSRVRESESYKHNENAAAAAQTQS 240
             G     +PK K+        + +   +S ++K+V   +   +S+  + +        +
Sbjct: 181 --GSKSFTKPKLKKSESKIFTFKNVFTNLSCSKKSVIKPINGFDSFDGSSDTDRYIPEIN 240

Query: 241 SVH--LSSHAS---LAQEDDGDYFLGGQSVQWAQGKAGEDRVHVVISEDNGWVFVGIYDG 300
           S+   +SSH       +ED  +  L    +QWAQGKAGEDRVHV++SE+NGW+FVGIYDG
Sbjct: 241 SLSTIVSSHEKPRIKEEEDKTESALEEPKIQWAQGKAGEDRVHVILSEENGWLFVGIYDG 300

Query: 301 FNGPDAPDYLLANLYTAVLKELKGLIWNDKFDSTATSSSMNSSNSASIEEDDGTTHMKNQ 360
           F+GPD PDYL+ NLYTAVL+ELKGL+W DK        S N +  ++IE+     H  + 
Sbjct: 301 FSGPDPPDYLIKNLYTAVLRELKGLLWIDK------GESYNRNGESNIEKQSTVEHASD- 360

Query: 361 GNDHQSTQVANGIVNLEADSARKRERVYNTNYRTSQLMKKADQNPKNWKCEWERERLEFD 420
            +D ++  V NG  N  A  +R                  +D     W+CEWE       
Sbjct: 361 -SDQENCPVMNG--NDVACGSRN---------------ITSDVKKLQWRCEWE------- 420

Query: 421 GKLNNRHLDHHQIWDESTAINHSEVLSALSQALRKTEEAYLQNADKMVTRNPELALMGSC 480
                     H   ++S  INH +VL AL QAL KTEE++    D MV  NPELALMGSC
Sbjct: 421 ----------HNSSNKSNNINHKDVLRALQQALEKTEESF----DLMVNENPELALMGSC 480

Query: 481 VLVMLMRGEDVYLMNVGDSRAIVAQQLEPDFGIGRINEGTLRVFESSN----GVEFERFT 540
           VLV LM+GEDVY+M+VGDSRA++A++  P+    ++ +   RV E S      +     +
Sbjct: 481 VLVTLMKGEDVYVMSVGDSRAVLARR--PNVEKMKMQKELERVKEESPLETLFITERGLS 540

Query: 541 TLASHQLTMDHSTYTEEEVQRIKNAHPDDTSAIMNDRVKGYLKITRAFGAGFLKQ----- 600
            L   QL  +HST  EEEV+RIK  HPDD  AI N+RVKGYLK+TRAFGAGFLKQ     
Sbjct: 541 LLVPVQLNKEHSTSVEEEVRRIKKEHPDDILAIENNRVKGYLKVTRAFGAGFLKQPKWNE 600

Query: 601 --------------------------------------------YFTNEEAVAHVESFIA 620
                                                       YF+NEEA+  V+SFI+
Sbjct: 601 ALLEMFRIDYVGTSPYITCSPSLHHHRLSSRDKFLILSSDGLYEYFSNEEAIFEVDSFIS 613

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038877629.10.0e+0084.55probable protein phosphatase 2C 23 [Benincasa hispida][more]
XP_023518015.10.0e+0084.33probable protein phosphatase 2C 23 [Cucurbita pepo subsp. pepo][more]
XP_022966555.10.0e+0084.05probable protein phosphatase 2C 23 [Cucurbita maxima][more]
KAG7027333.10.0e+0083.91putative protein phosphatase 2C 4, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6595324.10.0e+0083.91putative protein phosphatase 2C 4, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Q9LQN62.9e-15748.01Probable protein phosphatase 2C 4 OS=Arabidopsis thaliana OX=3702 GN=PLL5 PE=2 S... [more]
Q9ZV252.1e-15547.49Probable protein phosphatase 2C 23 OS=Arabidopsis thaliana OX=3702 GN=PLL4 PE=2 ... [more]
Q9LZ862.7e-13144.99Probable protein phosphatase 2C 66 OS=Arabidopsis thaliana OX=3702 GN=PLL2 PE=2 ... [more]
Q84T944.6e-12343.07Protein phosphatase 2C 35 OS=Oryza sativa subsp. japonica OX=39947 GN=XB15 PE=1 ... [more]
Q9SR242.4e-11942.44Probable protein phosphatase 2C 36 OS=Arabidopsis thaliana OX=3702 GN=PLL3 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HRY60.0e+0084.05probable protein phosphatase 2C 23 OS=Cucurbita maxima OX=3661 GN=LOC111466201 P... [more]
A0A6J1EZZ80.0e+0083.91probable protein phosphatase 2C 23 OS=Cucurbita moschata OX=3662 GN=LOC111438156... [more]
A0A5A7SN990.0e+0081.02PPM-type phosphatase domain-containing protein OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3BI010.0e+0081.02probable protein phosphatase 2C 4 OS=Cucumis melo OX=3656 GN=LOC103490267 PE=4 S... [more]
A0A6J1ISC51.2e-28076.45probable protein phosphatase 2C 23 OS=Cucurbita maxima OX=3661 GN=LOC111478861 P... [more]
Match NameE-valueIdentityDescription
AT1G07630.12.0e-15848.01pol-like 5 [more]
AT2G28890.11.5e-15647.49poltergeist like 4 [more]
AT5G02400.11.9e-13244.99pol-like 2 [more]
AT3G09400.11.7e-12042.44pol-like 3 [more]
AT3G09400.28.7e-10140.35pol-like 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001932PPM-type phosphatase domainSMARTSM00332PP2C_4coord: 241..645
e-value: 1.2E-32
score: 124.4
IPR001932PPM-type phosphatase domainPFAMPF00481PP2Ccoord: 414..588
e-value: 7.5E-18
score: 65.1
IPR001932PPM-type phosphatase domainPROSITEPS51746PPM_2coord: 252..647
score: 31.17407
IPR001932PPM-type phosphatase domainCDDcd00143PP2Cccoord: 407..647
e-value: 2.31494E-29
score: 115.116
IPR036457PPM-type phosphatase domain superfamilyGENE3D3.60.40.10coord: 217..652
e-value: 4.8E-68
score: 232.1
IPR036457PPM-type phosphatase domain superfamilySUPERFAMILY81606PP2C-likecoord: 263..648
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 153..186
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 322..358
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 322..338
NoneNo IPR availablePANTHERPTHR13832:SF785PHOSPHATASE 2C, PUTATIVE-RELATEDcoord: 20..579
NoneNo IPR availablePANTHERPTHR13832:SF785PHOSPHATASE 2C, PUTATIVE-RELATEDcoord: 579..654
IPR015655Protein phosphatase 2C familyPANTHERPTHR13832PROTEIN PHOSPHATASE 2Ccoord: 579..654
IPR015655Protein phosphatase 2C familyPANTHERPTHR13832PROTEIN PHOSPHATASE 2Ccoord: 20..579

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg013529.1Spg013529.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006470 protein dephosphorylation
molecular_function GO:0004722 protein serine/threonine phosphatase activity
molecular_function GO:0016791 phosphatase activity