Spg012522 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg012522
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein kinase domain-containing protein
Locationscaffold1: 16608023 .. 16612584 (+)
RNA-Seq ExpressionSpg012522
SyntenySpg012522
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGAGATGCAAAAAGGAAAAAAGGGAACCCCAAATGGACCCACTTACCCATTCTCAGCGCTCAAAAATTTGGAACTCACAAGTCAGAAGGCGAAGGGGATAAACTAGATAAAGCCACGCGGCCTCAGTCCAGCCACTGCCCTTCGCCACTCTCAGCACTCTGTAAAACGATGCAAACGCCACAGCCATTAACTCGACACCACGACGATGGCCTCTCTGTTCTACCCATTTGCAATTTCCCTTCAACAAAGCCCCCAAACCCTCTGCTTCTCTCCTCTTAAACCCACTCTCCAAACCGAGATCCCCCTCTTCGCCGGAGCTTTCAGGGCCAAGCAGCCGCCGGGCAAGTGCCGAGCATCATTCTTCGGCGATATATCCGATCATCTCATCGACAGCTTGGACGGCCCTCTGCGAGTCGATCAGCTTCCGGTTTTGCAATCTGGGTATGTTCAGTTTCAACGATTCTCTGGGGAATTGAGCGATTTTCAGAAATGGGGTTTCGTCGTTTTTGCTGGGATCATTTGGGTTTATCTCACTGCTCGACCAGGGGTTCTCTTTGGCGCCATTGATACGTACCTTCTGGCTCCTCTGCAGCTGGGGTTTGACAGTTTGTTGGGAAGAAGAAGCTTGAAGAGTTCTGATTTTGTAATTGGGGGGAAATTGGGAGAGGGTTCGTTTGGAGTTGTTTATTCGGGTGCGTTTCTTCGGAAGAATGTGAAGGTTGAGGAGCGAACGAGTAGAGGTCTGGAGTTGGATGGGAAGGAGAAAGTGATTCTTAAAAAGGTAACTCTTAATGCATTGGTTTTGGATGGCTGTTTCAAACTTCTTTAATGGTGTGTTTGAAAGTAGTTTAGTTAGAAGTGCTTCATAACATAGTGCTTTGATGGAACTGTTATTAGGATAGAATTAGGAATATTATTAGGACAGCAATTAGGATAGAATTAAGAATATTATTAGGATATTAAGAAAAGCAGTTTAGTCAGAAGTGCTTAATAACATGGTGCTTAAATAGGACGCTATTAGGATAGAATTAGGAATATTGTTAGGGTATCAAGGATATATTAGTAATTAGATAAAGAGTTTGTTTAGAGGGATTTGTTATAAATACAATGAGTGGGAAATGATGGAGGTAGGCAATGTTTGGTAAGTTTAGATTGGAGTTTGATTACTCAAGCGAGGTTACAAGTACCTCAAATACTCTTGAAAATACATTTGAATGGTTTTTTTTTTGTTAAGTATTTATCCAAAAGCACTCTACAGGTGCTTTTTCACGAAACGCTTTAAGTAGTTTTGACTTCTTCCAGAAACACTCTTTTATGTTAATATTTTGAGGGAGAAGTGCTTTTGGCATAGCAAAAGTACTTTTGAACACTCCCAAACACACCTTAGATATGAAAAGAACAAATGAAGATGCGGTTTGGTTTGATTTGTCTGATTGTCATTTGCAGATTAAGGTGGGAGTTAAAGGAGCTGAAAAGTTTGGGGAATATGAGGAGTGGTTCAACTACAGACTTTCAAGAGCGGCGCCTGAAACTTGTGCTGAGTTCCTTGGAAGTTTTGTAGCTAACAGAGAAAGCAAGCAGTTCTCTGCAGGTGGAAAATGGCTCGTTTGGAAATTTGAGGTAACTAACAAGTTTAAGGGTCCGTTTGGAATACATTTTAATTTAAATGTTTTTCAAAAATGCATTTTGTAAAAAACATTTTGTATAAAAATTGTTTAGAATTTAAACACTCATGTGTTTGGTTGCATTTACCCACAAATGTTTTTATACACAAGTTATTGTTTACTTAAGAAGTCTTTTTTAAAAAGTTGGTTTCAAGTGTTTCTCTCAAAATGGTTTATTTATCAATCTCATTTTCTAATAAGTCATTTTTAGTGGTTGCCAAACACTAGAAATTTTTTCAAAATGATTTATTTTCAAAATTAAACACTTGAAAAGTTAAACCAAACACACCCTAAATATGTCAAAGTGCTTTTGATTTCATTATATCGATGCATCACAAAAGTTAAAAAAAGAAACCTTAAAATATTTTCAATTTTTAAAAATGAACTAAGTGAGAGAAAGTGTAGAGAGAGAGAAAGAGATAAGTGAGAAAAATTGCATGTGTCTAGGGAGGCTAATAGAAAATGTGTGTGGAGAGAGTGTGTATAGAGAAGTTGGTATGAGAAAATATTTGGAGAGTGATTAGCAAAAGAGAGAGAGCAGAGCGTCTTGAAATCAAAAGCACTTGATAAAGCAAATTCATTTATTTCCTTGAAAAGTACTTCTAAATGGTTTAGACTTTTATAATGATAGGGTGTTTCGTGGTCTTGAGAAGGTTCCTCCCGACGTTTGGGCCCTTACTAGATTCCATGTTTCTCTCTGGGCCTTAGTTTCTTAGTCTTTTTGTATTATTCTTTAGGCACTATTATTCTTGATTGGCGTCCCTCCCTTTAAAGGGGTCTTGTTGTAGGCTTGTTTTTTTTTATGCCCTTGTATTCTTTCATTTTTTTGCAATGAAAGTTGGTTTTATTATTAAAAAAATGGTTTAGACTTTTATGAAACTCCTTCTAAATTGAAGCCCAACATAAATATTTGAGTTATAAAATACTTTTAACAAACCAGCATTGTAATTTTTTGAACTTGAGATGTCATAACATTTTAAACTAACTCTAAACATAAAAGCTGCTTAAGTTATTAGTCTTTGGAGGCAACTTCCAAAACCATTGTGTTTGATCTCAAGGATGGTGAATATGATAGGGAGATCAGACTCTTGGTGATTACATGAAAGACAGAAGCTTTCCTGTAAACATGGAAGCTCTTATGTTCGGACGTGTCTTACAAGGCATGAACTCGGTCGAAAGAAACGCATTGATAATCAAGCAAATCCTGCGCCAAATCATCACATCATTGAAGAAGATCCATGACACAGGCATTGTTCACAGGGATGTAAAGCCAGCAAACTTAGTAGTAACCAAAAAAGGACAGGTAAAGATTATAGATTTCGGGGCAGCGACCGACCTCCGAATCGGCAAGAACTACGTACCGAATCTTGCTTTGCTGGATCCTGACTACTGTCCTCCTGAGCTTTATGTGATGCCTGAAGAAACACCTAGCCCTCCTCCAGCTCCCATTGCAGCACTGCTTTCTCCTATACTCTGGCAGGTAAGCTGATCTCCATGGATCTTTTCTTGAAGCTGAAAGGTTGGATTCTGATGCTTATTAGCAAGATGTCTGTGAAACAAAAGTTCAGTTTATCATATGCTTAAGTTACAAAGTTAGTCATAATACGTCATTGAATTTTAAAAAATATCTAATAGGTTCCTAAACTTTCAATTTTTTGTCTCATAGGTTCCTAAACTTTGAGACCTTTAAAAATATCAAATATAATAAGTTCCTAAACTTTCAATTTTGTTTCTATTAAGTCCCTAGCATATTCAACAATTTTCAAACTTGAGAACTCAGAGATTTATTAGACATAGAATCAATATCTTTTTTTTTTTTTTTTTTTGATAAGAAACAATTTCATTGATGTATGAAATTTACAAAAGGGATACAAGATCCATAAGAGTTACAAAAGGCTTTCCCAACTATTGATGAGGGAAGCATATGAGTATGATGATAGTAAGGGAGAGAGTTTACACCAAGATATAGCCAAGAATATAACAGAATCAAAAAAGGCTTCAAAGGAGGACTCCATTCCTTGAAAAGTTCTTTTATTTCTTTCTCTCCATAAACTCCAATAAAAGGCTCTGATGATGTTCATCCATAACAGTGCTTTAAGACATAGAATCAATATCTTAGAGACTTATTAGACACTTTTTAAAATTCGGAGGCCTATTTGACACAAACTTAAAAGTTCAGGGTCTAGACTTGCAATTTAACGAAATCATATTTATTTATGGATCATCTATAGAATGATCCATGCTTGTAAAAGGCTGCTGTGGACTTTCCATTGCATATTTTTGAAGTATTCCAATTTGGAAACTGTTTTTGAAACTGTTTTGTTATTGCAGCTGAACAGCCCTGATTTGTTTGACATGTATTCTGCTGGTATTGTGCTTATGCAAATGGCAGTGCCAAACTTGAGGAGTAGTGCTGGGTTGAAGAACTTCAATTTGGAAATTAAGAACTGTGGATATGACTTGAATAAATGGAGGGAGAAAACAAGAACAAAGCCCGATTTGACGATACTCGATCTCGACTCAGGGAGAGGGTGGGATCTCGCAACGAAGCTCGTTTCGGAGAGAGGCTTCCTTAGAAGGGGGCGTTTATCGGCTTCCGCTGCTCTGAGGCATCCTTATTTCTTGTTGGGTGGTGATCAGGCAGCTGCAGTTATTTCAAAACTAAACTTGGTCAAGAAATGAGGTCATAGGAGGGAATGTAAGTAGAAATTTATATATATTATATTCATTTTTCATCTTGATCTGGTTTTTCTTATTCAATTTCATGTTAGAGCAATGTCTTCAATGCTAAATTTTGACTTTGAGAGAAAAAATCAATGCAAAAGTAACTTGATTAGGTAAACATTCTATCATGTTTGGCTACTGATTAGTATTCTTGAGCTGGAGATGTTTTTCAG

mRNA sequence

ATGGCCTCTCTGTTCTACCCATTTGCAATTTCCCTTCAACAAAGCCCCCAAACCCTCTGCTTCTCTCCTCTTAAACCCACTCTCCAAACCGAGATCCCCCTCTTCGCCGGAGCTTTCAGGGCCAAGCAGCCGCCGGGCAAGTGCCGAGCATCATTCTTCGGCGATATATCCGATCATCTCATCGACAGCTTGGACGGCCCTCTGCGAGTCGATCAGCTTCCGGTTTTGCAATCTGGGTATGTTCAGTTTCAACGATTCTCTGGGGAATTGAGCGATTTTCAGAAATGGGGTTTCGTCGTTTTTGCTGGGATCATTTGGGTTTATCTCACTGCTCGACCAGGGGTTCTCTTTGGCGCCATTGATACGTACCTTCTGGCTCCTCTGCAGCTGGGGTTTGACAGTTTGTTGGGAAGAAGAAGCTTGAAGAGTTCTGATTTTGTAATTGGGGGGAAATTGGGAGAGGGTTCGTTTGGAGTTGTTTATTCGGGTGCGTTTCTTCGGAAGAATGTGAAGGTTGAGGAGCGAACGAGTAGAGGTCTGGAGTTGGATGGGAAGGAGAAAGTGATTCTTAAAAAGATTAAGGTGGGAGTTAAAGGAGCTGAAAAGTTTGGGGAATATGAGGAGTGGTTCAACTACAGACTTTCAAGAGCGGCGCCTGAAACTTGTGCTGAGTTCCTTGGAAGTTTTGTAGCTAACAGAGAAAGCAAGCAGTTCTCTGCAGGTGGAAAATGGCTCGTTTGGAAATTTGAGGGAGATCAGACTCTTGGTGATTACATGAAAGACAGAAGCTTTCCTGTAAACATGGAAGCTCTTATGTTCGGACGTGTCTTACAAGGCATGAACTCGGTCGAAAGAAACGCATTGATAATCAAGCAAATCCTGCGCCAAATCATCACATCATTGAAGAAGATCCATGACACAGGCATTGTTCACAGGGATGTAAAGCCAGCAAACTTAGTAGTAACCAAAAAAGGACAGGTAAAGATTATAGATTTCGGGGCAGCGACCGACCTCCGAATCGGCAAGAACTACGTACCGAATCTTGCTTTGCTGGATCCTGACTACTGTCCTCCTGAGCTTTATGTGATGCCTGAAGAAACACCTAGCCCTCCTCCAGCTCCCATTGCAGCACTGCTTTCTCCTATACTCTGGCAGCTGAACAGCCCTGATTTGTTTGACATGTATTCTGCTGGTATTGTGCTTATGCAAATGGCAGTGCCAAACTTGAGGAGTAGTGCTGGGTTGAAGAACTTCAATTTGGAAATTAAGAACTGTGGATATGACTTGAATAAATGGAGGGAGAAAACAAGAACAAAGCCCGATTTGACGATACTCGATCTCGACTCAGGGAGAGGGTGGGATCTCGCAACGAAGCTCGTTTCGGAGAGAGGCTTCCTTAGAAGGGGGCGTTTATCGGCTTCCGCTGCTCTGAGGCATCCTTATTTCTTGTTGGGTGGTGATCAGGCAGCTGCAGTTATTTCAAAACTAAACTTGGTCAAGAAATGA

Coding sequence (CDS)

ATGGCCTCTCTGTTCTACCCATTTGCAATTTCCCTTCAACAAAGCCCCCAAACCCTCTGCTTCTCTCCTCTTAAACCCACTCTCCAAACCGAGATCCCCCTCTTCGCCGGAGCTTTCAGGGCCAAGCAGCCGCCGGGCAAGTGCCGAGCATCATTCTTCGGCGATATATCCGATCATCTCATCGACAGCTTGGACGGCCCTCTGCGAGTCGATCAGCTTCCGGTTTTGCAATCTGGGTATGTTCAGTTTCAACGATTCTCTGGGGAATTGAGCGATTTTCAGAAATGGGGTTTCGTCGTTTTTGCTGGGATCATTTGGGTTTATCTCACTGCTCGACCAGGGGTTCTCTTTGGCGCCATTGATACGTACCTTCTGGCTCCTCTGCAGCTGGGGTTTGACAGTTTGTTGGGAAGAAGAAGCTTGAAGAGTTCTGATTTTGTAATTGGGGGGAAATTGGGAGAGGGTTCGTTTGGAGTTGTTTATTCGGGTGCGTTTCTTCGGAAGAATGTGAAGGTTGAGGAGCGAACGAGTAGAGGTCTGGAGTTGGATGGGAAGGAGAAAGTGATTCTTAAAAAGATTAAGGTGGGAGTTAAAGGAGCTGAAAAGTTTGGGGAATATGAGGAGTGGTTCAACTACAGACTTTCAAGAGCGGCGCCTGAAACTTGTGCTGAGTTCCTTGGAAGTTTTGTAGCTAACAGAGAAAGCAAGCAGTTCTCTGCAGGTGGAAAATGGCTCGTTTGGAAATTTGAGGGAGATCAGACTCTTGGTGATTACATGAAAGACAGAAGCTTTCCTGTAAACATGGAAGCTCTTATGTTCGGACGTGTCTTACAAGGCATGAACTCGGTCGAAAGAAACGCATTGATAATCAAGCAAATCCTGCGCCAAATCATCACATCATTGAAGAAGATCCATGACACAGGCATTGTTCACAGGGATGTAAAGCCAGCAAACTTAGTAGTAACCAAAAAAGGACAGGTAAAGATTATAGATTTCGGGGCAGCGACCGACCTCCGAATCGGCAAGAACTACGTACCGAATCTTGCTTTGCTGGATCCTGACTACTGTCCTCCTGAGCTTTATGTGATGCCTGAAGAAACACCTAGCCCTCCTCCAGCTCCCATTGCAGCACTGCTTTCTCCTATACTCTGGCAGCTGAACAGCCCTGATTTGTTTGACATGTATTCTGCTGGTATTGTGCTTATGCAAATGGCAGTGCCAAACTTGAGGAGTAGTGCTGGGTTGAAGAACTTCAATTTGGAAATTAAGAACTGTGGATATGACTTGAATAAATGGAGGGAGAAAACAAGAACAAAGCCCGATTTGACGATACTCGATCTCGACTCAGGGAGAGGGTGGGATCTCGCAACGAAGCTCGTTTCGGAGAGAGGCTTCCTTAGAAGGGGGCGTTTATCGGCTTCCGCTGCTCTGAGGCATCCTTATTTCTTGTTGGGTGGTGATCAGGCAGCTGCAGTTATTTCAAAACTAAACTTGGTCAAGAAATGA

Protein sequence

MASLFYPFAISLQQSPQTLCFSPLKPTLQTEIPLFAGAFRAKQPPGKCRASFFGDISDHLIDSLDGPLRVDQLPVLQSGYVQFQRFSGELSDFQKWGFVVFAGIIWVYLTARPGVLFGAIDTYLLAPLQLGFDSLLGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLRKNVKVEERTSRGLELDGKEKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVANRESKQFSAGGKWLVWKFEGDQTLGDYMKDRSFPVNMEALMFGRVLQGMNSVERNALIIKQILRQIITSLKKIHDTGIVHRDVKPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPELYVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNLEIKNCGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSASAALRHPYFLLGGDQAAAVISKLNLVKK
Homology
BLAST of Spg012522 vs. NCBI nr
Match: XP_038881696.1 (serine/threonine-protein kinase STN8, chloroplastic [Benincasa hispida])

HSP 1 Score: 907.9 bits (2345), Expect = 3.9e-260
Identity = 461/501 (92.02%), Postives = 474/501 (94.61%), Query Frame = 0

Query: 1   MASLFYPFAISLQQSPQTLCFSPLKPTLQTEIPLFAGAFRAKQPPGKCRASFFGDISDHL 60
           MASL +PFAISLQQS QT  FSPLKP LQTEIPLFAG FRAK P  KCRASF  DISD L
Sbjct: 1   MASLIFPFAISLQQSSQTR-FSPLKPALQTEIPLFAGTFRAKPPASKCRASFLVDISDRL 60

Query: 61  IDSLDGPLRVDQLPVLQSGYVQFQRFSGELSDFQKWGFVVFAGIIWVYLTARPGVLFGAI 120
           +D+L GPLRVDQLP LQSGYVQFQRFSGELSD QKWGFVVFAG IWVYLTARPGVL GAI
Sbjct: 61  LDNLYGPLRVDQLPGLQSGYVQFQRFSGELSDLQKWGFVVFAGFIWVYLTARPGVLVGAI 120

Query: 121 DTYLLAPLQLGFDSLLGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLRKNVKVEERTSRGL 180
           DTYLLAPLQLG DSL+GRRSLKSSDFVIGGKLGEGSFGVVYSGAFL KNVK E RTSR  
Sbjct: 121 DTYLLAPLQLGLDSLMGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKNVKNEGRTSRA- 180

Query: 181 ELDGKEKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVANRESKQFSA 240
           ELDGK+KVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCA+FLGSFVA+RESKQFSA
Sbjct: 181 ELDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCADFLGSFVADRESKQFSA 240

Query: 241 GGKWLVWKFEGDQTLGDYMKDRSFPVNMEALMFGRVLQGMNSVERNALIIKQILRQIITS 300
           GGKWLVWKFEGDQTLGDYMKDR+FPVN+EALMFGRVLQGMNSVERNALIIKQILRQIITS
Sbjct: 241 GGKWLVWKFEGDQTLGDYMKDRNFPVNLEALMFGRVLQGMNSVERNALIIKQILRQIITS 300

Query: 301 LKKIHDTGIVHRDVKPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL 360
           LKKIHDTGIVHRDVKPAN+VVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPD+CPPEL
Sbjct: 301 LKKIHDTGIVHRDVKPANIVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDFCPPEL 360

Query: 361 YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNL 420
           YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFN+
Sbjct: 361 YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNM 420

Query: 421 EIKNCGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSASAALRHP 480
           EIKN GYDLNKWREKTRTKPDLTIL+LDSGRGWDL TKLVSERG LRRGRLSASAALRHP
Sbjct: 421 EIKNYGYDLNKWREKTRTKPDLTILNLDSGRGWDLVTKLVSERGTLRRGRLSASAALRHP 480

Query: 481 YFLLGGDQAAAVISKLNLVKK 502
           YFLLG DQAAAVISKLNLVKK
Sbjct: 481 YFLLGSDQAAAVISKLNLVKK 499

BLAST of Spg012522 vs. NCBI nr
Match: XP_022950141.1 (serine/threonine-protein kinase STN8, chloroplastic [Cucurbita moschata])

HSP 1 Score: 907.1 bits (2343), Expect = 6.6e-260
Identity = 453/501 (90.42%), Postives = 476/501 (95.01%), Query Frame = 0

Query: 1   MASLFYPFAISLQQSPQTLCFSPLKPTLQTEIPLFAGAFRAKQPPGKCRASFFGDISDHL 60
           MASL +PFAISLQQ+ QT  FSPLKP L TEIPLFAG FR K P GKCRASFFGDISD L
Sbjct: 1   MASLIFPFAISLQQTHQTR-FSPLKPILPTEIPLFAGTFRPKPPAGKCRASFFGDISDDL 60

Query: 61  IDSLDGPLRVDQLPVLQSGYVQFQRFSGELSDFQKWGFVVFAGIIWVYLTARPGVLFGAI 120
           +++LDGPLRVDQLPVLQSGYVQFQRFSGELSD QKWGF+VFAG IWVYLTARPGVLFGAI
Sbjct: 61  LNNLDGPLRVDQLPVLQSGYVQFQRFSGELSDLQKWGFIVFAGFIWVYLTARPGVLFGAI 120

Query: 121 DTYLLAPLQLGFDSLLGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLRKNVKVEERTSRGL 180
           DTYLLAPLQLG  SL+GRR+LKSSDFVIG KLGEGSFGVVYSGAFL KNVK+E RTS+ L
Sbjct: 121 DTYLLAPLQLGLYSLIGRRNLKSSDFVIGSKLGEGSFGVVYSGAFLPKNVKIENRTSKVL 180

Query: 181 ELDGKEKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVANRESKQFSA 240
           ELDGK+KVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVA+RE++QFSA
Sbjct: 181 ELDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRENRQFSA 240

Query: 241 GGKWLVWKFEGDQTLGDYMKDRSFPVNMEALMFGRVLQGMNSVERNALIIKQILRQIITS 300
           GGKWLVWKFEGDQTLGDYMKDRSFP+N+E LMF RVLQGMNS+ERNALIIKQILRQIITS
Sbjct: 241 GGKWLVWKFEGDQTLGDYMKDRSFPLNLETLMFRRVLQGMNSIERNALIIKQILRQIITS 300

Query: 301 LKKIHDTGIVHRDVKPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL 360
           LKKIHDTGIVHRDVKPANLVVTKKGQ+KIIDFGAATDLRIGKNY+PNLALLDPDYCPPEL
Sbjct: 301 LKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYIPNLALLDPDYCPPEL 360

Query: 361 YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNL 420
           YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVP+LRSSAGLKNFN+
Sbjct: 361 YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPSLRSSAGLKNFNM 420

Query: 421 EIKNCGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSASAALRHP 480
           EIKN GYDLN+WRE+TRTKPDLTILDLDS RGWDLATKLVSERG LRRGRLSASAALRHP
Sbjct: 421 EIKNYGYDLNEWRERTRTKPDLTILDLDSRRGWDLATKLVSERGSLRRGRLSASAALRHP 480

Query: 481 YFLLGGDQAAAVISKLNLVKK 502
           YFLLG DQAAAVISKLNLVKK
Sbjct: 481 YFLLGSDQAAAVISKLNLVKK 500

BLAST of Spg012522 vs. NCBI nr
Match: XP_023545193.1 (serine/threonine-protein kinase STN8, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 907.1 bits (2343), Expect = 6.6e-260
Identity = 453/501 (90.42%), Postives = 476/501 (95.01%), Query Frame = 0

Query: 1   MASLFYPFAISLQQSPQTLCFSPLKPTLQTEIPLFAGAFRAKQPPGKCRASFFGDISDHL 60
           MASL +PFAISLQQ+ QT  FSPLKP L TEIPLFAG FR K P GKCRASFFGDISD L
Sbjct: 1   MASLIFPFAISLQQTHQTR-FSPLKPILPTEIPLFAGTFRPKPPAGKCRASFFGDISDDL 60

Query: 61  IDSLDGPLRVDQLPVLQSGYVQFQRFSGELSDFQKWGFVVFAGIIWVYLTARPGVLFGAI 120
           +++LDGPLRVDQLPVLQSGYVQFQRFSGELSD QKWGF+VFAG IWVYLTARPGVLFGAI
Sbjct: 61  LNNLDGPLRVDQLPVLQSGYVQFQRFSGELSDLQKWGFIVFAGFIWVYLTARPGVLFGAI 120

Query: 121 DTYLLAPLQLGFDSLLGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLRKNVKVEERTSRGL 180
           DTYLLAPLQLG  SL+GRR+LKSSDFVIG KLGEGSFGVVYSGAFL KNVK+E RTS+ L
Sbjct: 121 DTYLLAPLQLGLYSLIGRRNLKSSDFVIGSKLGEGSFGVVYSGAFLPKNVKIENRTSKVL 180

Query: 181 ELDGKEKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVANRESKQFSA 240
           ELDGK+KVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVA+RE++QFSA
Sbjct: 181 ELDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRENRQFSA 240

Query: 241 GGKWLVWKFEGDQTLGDYMKDRSFPVNMEALMFGRVLQGMNSVERNALIIKQILRQIITS 300
           GGKWLVWKFEGDQTLGDYMKDRSFP+N+E LMF RVLQGMNS+ERNALIIKQILRQIITS
Sbjct: 241 GGKWLVWKFEGDQTLGDYMKDRSFPLNLETLMFRRVLQGMNSIERNALIIKQILRQIITS 300

Query: 301 LKKIHDTGIVHRDVKPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL 360
           LKKIHDTGIVHRDVKPANLVVTKKGQ+KIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL
Sbjct: 301 LKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL 360

Query: 361 YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNL 420
           YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVP+LRSSAGLKNFN+
Sbjct: 361 YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPSLRSSAGLKNFNM 420

Query: 421 EIKNCGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSASAALRHP 480
           EIKN GYDLN+WRE+TRTKPDLTILDLDS RGWDLATKL+SERG LRRGRLSASAALRHP
Sbjct: 421 EIKNYGYDLNEWRERTRTKPDLTILDLDSRRGWDLATKLISERGSLRRGRLSASAALRHP 480

Query: 481 YFLLGGDQAAAVISKLNLVKK 502
           YFLLG DQAAAVISKLNLVKK
Sbjct: 481 YFLLGSDQAAAVISKLNLVKK 500

BLAST of Spg012522 vs. NCBI nr
Match: KAG6603530.1 (Serine/threonine-protein kinase STN8, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 905.6 bits (2339), Expect = 1.9e-259
Identity = 453/501 (90.42%), Postives = 475/501 (94.81%), Query Frame = 0

Query: 1   MASLFYPFAISLQQSPQTLCFSPLKPTLQTEIPLFAGAFRAKQPPGKCRASFFGDISDHL 60
           MASL +PFAISLQQ+ QT  FSPLKP L TEIPLFAG FR K P GKCRASFFGDISD L
Sbjct: 1   MASLIFPFAISLQQTHQTR-FSPLKPILPTEIPLFAGTFRPKPPSGKCRASFFGDISDDL 60

Query: 61  IDSLDGPLRVDQLPVLQSGYVQFQRFSGELSDFQKWGFVVFAGIIWVYLTARPGVLFGAI 120
           +++LDGPLRVDQLPVLQSGYVQFQRFSGELSD QKWGF+VFAG IWVYLTARPGVLFGAI
Sbjct: 61  LNNLDGPLRVDQLPVLQSGYVQFQRFSGELSDLQKWGFIVFAGFIWVYLTARPGVLFGAI 120

Query: 121 DTYLLAPLQLGFDSLLGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLRKNVKVEERTSRGL 180
           DTYLLAPLQLG  SL+GRR+LKSSDFVIG KLGEGSFGVVYSGAFL KNVK+E RTS+ L
Sbjct: 121 DTYLLAPLQLGLYSLIGRRNLKSSDFVIGSKLGEGSFGVVYSGAFLPKNVKIENRTSKVL 180

Query: 181 ELDGKEKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVANRESKQFSA 240
           ELDGK+KVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVA+RE++QFSA
Sbjct: 181 ELDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRENRQFSA 240

Query: 241 GGKWLVWKFEGDQTLGDYMKDRSFPVNMEALMFGRVLQGMNSVERNALIIKQILRQIITS 300
           GGKWLVWKFEGDQTLGDYMKDRSFP+N+E LMF RVLQGMNS+ERNALIIKQILRQIITS
Sbjct: 241 GGKWLVWKFEGDQTLGDYMKDRSFPLNLETLMFRRVLQGMNSIERNALIIKQILRQIITS 300

Query: 301 LKKIHDTGIVHRDVKPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL 360
           LKKIHDTGIVHRDVKPANLVVTKKGQ+KIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL
Sbjct: 301 LKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL 360

Query: 361 YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNL 420
           YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVP+LRSSAGLKNFN+
Sbjct: 361 YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPSLRSSAGLKNFNM 420

Query: 421 EIKNCGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSASAALRHP 480
           EIKN GYDLN+WRE+TRTKPDLTILDLDS RGWDLATKLVSERG LRRGRLSASAALRHP
Sbjct: 421 EIKNYGYDLNEWRERTRTKPDLTILDLDSRRGWDLATKLVSERGSLRRGRLSASAALRHP 480

Query: 481 YFLLGGDQAAAVISKLNLVKK 502
           YFLLG DQAAAVISKLNLV K
Sbjct: 481 YFLLGSDQAAAVISKLNLVNK 500

BLAST of Spg012522 vs. NCBI nr
Match: KAG7033713.1 (Serine/threonine-protein kinase STN8, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 905.6 bits (2339), Expect = 1.9e-259
Identity = 453/501 (90.42%), Postives = 475/501 (94.81%), Query Frame = 0

Query: 1   MASLFYPFAISLQQSPQTLCFSPLKPTLQTEIPLFAGAFRAKQPPGKCRASFFGDISDHL 60
           MASL +PFAISLQQ+ QT  FSPLKP L TEIPLFAG FR K P GKCRASFFGDISD L
Sbjct: 1   MASLIFPFAISLQQTHQTR-FSPLKPILPTEIPLFAGTFRPKPPAGKCRASFFGDISDDL 60

Query: 61  IDSLDGPLRVDQLPVLQSGYVQFQRFSGELSDFQKWGFVVFAGIIWVYLTARPGVLFGAI 120
           +++LDGPLRVDQLPVLQSGYVQFQRFSGELSD QKWGF+VFAG IWVYLTARPGVLFGAI
Sbjct: 61  LNNLDGPLRVDQLPVLQSGYVQFQRFSGELSDLQKWGFIVFAGFIWVYLTARPGVLFGAI 120

Query: 121 DTYLLAPLQLGFDSLLGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLRKNVKVEERTSRGL 180
           DTYLLAPLQLG  SL+GRR+LKSSDFVIG KLGEGSFGVVYSGAFL KNVK+E RTS+ L
Sbjct: 121 DTYLLAPLQLGLYSLIGRRNLKSSDFVIGSKLGEGSFGVVYSGAFLPKNVKIENRTSKVL 180

Query: 181 ELDGKEKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVANRESKQFSA 240
           ELDGK+KVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVA+RE++QFSA
Sbjct: 181 ELDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRENRQFSA 240

Query: 241 GGKWLVWKFEGDQTLGDYMKDRSFPVNMEALMFGRVLQGMNSVERNALIIKQILRQIITS 300
           GGKWLVWKFEGDQTLGDYMKDRSFP+N+E LMF RVLQGMNS+ERNALIIKQILRQIITS
Sbjct: 241 GGKWLVWKFEGDQTLGDYMKDRSFPLNLETLMFRRVLQGMNSIERNALIIKQILRQIITS 300

Query: 301 LKKIHDTGIVHRDVKPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL 360
           LKKIHDTGIVHRDVKPANLVVTKKGQ+KIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL
Sbjct: 301 LKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL 360

Query: 361 YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNL 420
           YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVP+LRSSAGLKNFN+
Sbjct: 361 YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPSLRSSAGLKNFNM 420

Query: 421 EIKNCGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSASAALRHP 480
           EIKN GYDLN+WRE+TRTKPDLTILDLDS RGWDLATKLVSERG LRRGRLSASAALRHP
Sbjct: 421 EIKNYGYDLNEWRERTRTKPDLTILDLDSRRGWDLATKLVSERGSLRRGRLSASAALRHP 480

Query: 481 YFLLGGDQAAAVISKLNLVKK 502
           YFLLG DQAAAVISKLNLV K
Sbjct: 481 YFLLGSDQAAAVISKLNLVNK 500

BLAST of Spg012522 vs. ExPASy Swiss-Prot
Match: Q9LZV4 (Serine/threonine-protein kinase STN8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=STN8 PE=2 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 3.1e-188
Identity = 324/429 (75.52%), Postives = 372/429 (86.71%), Query Frame = 0

Query: 80  YVQFQRFSGELSDFQKWGFVVFAGIIWVYLTARPGVLFGAIDTYLLAPLQLGFDSLLGRR 139
           ++ FQ      SD QKWGF V AGI+W YLTARPGVL GAID YLLAPLQLG D+L+GRR
Sbjct: 68  FLDFQNSLATFSDTQKWGFFVSAGIVWFYLTARPGVLIGAIDAYLLAPLQLGLDTLIGRR 127

Query: 140 SLKSSDFVIGGKLGEGSFGVVYSGAFLRKN------VKVEERTSRGLELDG--KEKVILK 199
            LK SDF++  KLGEGSFGVVY+G  L KN      V+V +  ++ ++  G  K++VILK
Sbjct: 128 -LKRSDFLVTEKLGEGSFGVVYAGVLLPKNSTLVDDVRVSKARAKAMDFTGEFKQRVILK 187

Query: 200 KIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVANRESKQFSAGGKWLVWKFEG 259
           K+KVGV+GAE+FGEYEEWFNYRLSRAAP+TCAEFLGSFVA++ +  F+ GGKWLVW+FEG
Sbjct: 188 KVKVGVRGAEEFGEYEEWFNYRLSRAAPDTCAEFLGSFVADKTNTMFTKGGKWLVWRFEG 247

Query: 260 DQTLGDYMKDRSFPVNMEALMFGRVLQGMNSVERNALIIKQILRQIITSLKKIHDTGIVH 319
           D+ L DYMKDRSFP N+E++MFGRVLQG+ SV+R ALIIKQI+RQIITSL+KIH TGIVH
Sbjct: 248 DRDLADYMKDRSFPSNLESIMFGRVLQGVESVKRRALIIKQIMRQIITSLRKIHGTGIVH 307

Query: 320 RDVKPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPELYVMPEETPSPP 379
           RDVKPANLVVTKKGQ+K+IDFGAA DLRIGKNY+P   LLDPDYCPPELYV+PEETPSPP
Sbjct: 308 RDVKPANLVVTKKGQIKLIDFGAAADLRIGKNYIPERTLLDPDYCPPELYVLPEETPSPP 367

Query: 380 PAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNLEIKNCGYDLNK 439
           P PIAALLSPILWQLNSPDLFDMYSAGIVL+QMAVP LRS+AGLKNFNLEIK+  YDLN+
Sbjct: 368 PEPIAALLSPILWQLNSPDLFDMYSAGIVLLQMAVPTLRSTAGLKNFNLEIKSVEYDLNR 427

Query: 440 WREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSASAALRHPYFLLGGDQAAA 499
           WRE+TRT+PDL+ILDLDSGRGWDL TKL+SERG LRRGRLSA+AALRHPYFLLGGDQAAA
Sbjct: 428 WRERTRTRPDLSILDLDSGRGWDLVTKLISERGSLRRGRLSAAAALRHPYFLLGGDQAAA 487

Query: 500 VISKLNLVK 501
           V+SKL+  K
Sbjct: 488 VLSKLSFSK 495

BLAST of Spg012522 vs. ExPASy Swiss-Prot
Match: Q9S713 (Serine/threonine-protein kinase STN7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=STN7 PE=1 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 4.8e-80
Identity = 170/398 (42.71%), Postives = 237/398 (59.55%), Query Frame = 0

Query: 108 YLTARPGVLFGAIDTYLLAPLQLGFDSLLGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLR 167
           YL A PGV  G  D ++LA     F   L R + +  DFV+G KLGEGSFGVVY  +  +
Sbjct: 101 YLWATPGVAPGFFDMFVLA-----FVERLFRPTFRKDDFVVGKKLGEGSFGVVYKVSLSK 160

Query: 168 KNVKVEERTSRGLELDGKEKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLG 227
           K    E             + +LKK       A ++G  E W N R+ RA   +CA+F+ 
Sbjct: 161 KRSNEE------------GEYVLKK-------ATEYGAVEIWMNERVRRACGNSCADFVY 220

Query: 228 SFVANRESKQFSAGGKWLVWKFEGDQTLGDYMKDRSFPVNMEALMFGRVLQGMNSVERNA 287
            F+ ++ SK+      WL+WK+EG+ TL   M+ + FP N+E ++ G+V      +ER  
Sbjct: 221 GFL-DKSSKK--GPEYWLLWKYEGESTLAGLMQSKEFPYNVETIILGKVQDLPKGLEREN 280

Query: 288 LIIKQILRQIITSLKKIHDTGIVHRDVKPANLVVTKKGQ-VKIIDFGAATDLRIGKNYVP 347
            II+ I+RQ++ +L  +H TGI+HRDVKP N++ ++  +  KIID GAA DLR+G NY+P
Sbjct: 281 KIIQTIMRQLLFALDGLHSTGIIHRDVKPQNIIFSEGSRSFKIIDLGAAADLRVGINYIP 340

Query: 348 NLALLDPDYCPPELYVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAV 407
              LLDP Y  PE Y+M  +TPS P AP+AA LSP+LWQ+N PD FD+YS G++ +QMA 
Sbjct: 341 KEFLLDPRYAAPEQYIMSTQTPSAPSAPVAAALSPVLWQMNLPDRFDIYSIGLIFLQMAF 400

Query: 408 PNLRSSAGLKNFNLEIKNCGYDLNKWRE--KTRTKPDL----TILDLDSGRGWDLATKLV 467
           P+LRS + L  FN ++K C YDL  WR+  + R   DL     ++DLD G GW+L T +V
Sbjct: 401 PSLRSDSNLIQFNRQLKRCDYDLTAWRKLVEPRASADLRRGFELVDLDGGIGWELLTSMV 460

Query: 468 SERGFLRRGRLSASAALRHPYFLLGGDQAAAVISKLNL 499
               +  R R+SA AAL HPYF   G  A +V+  L +
Sbjct: 461 R---YKARQRISAKAALAHPYFDRQGLLALSVMQNLRM 468

BLAST of Spg012522 vs. ExPASy Swiss-Prot
Match: Q84V18 (Serine/threonine-protein kinase stt7, chloroplastic OS=Chlamydomonas reinhardtii OX=3055 GN=STT7 PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 8.2e-72
Identity = 163/423 (38.53%), Postives = 226/423 (53.43%), Query Frame = 0

Query: 105 IWVYLTARPGVLFGAIDTYLLAPLQLGFDSLLGRRSLKSSDFVIGGKLGEGSFGVVYSGA 164
           +  YL   PGVL GAID Y+ APL+         +++  +D V+G +LG G FG V+ G 
Sbjct: 103 VLAYLFLPPGVLPGAIDYYIRAPLK-----RKQTKAIDKNDIVLGKRLGTGGFGTVFKG- 162

Query: 165 FLRKNVKVEERTSRGLELDGKEKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAE 224
                   E +   G+    K  +I+KK       A++FGE E W N R+SR A    AE
Sbjct: 163 --------ELKEEGGV----KTSIIIKK-------AKEFGEAEVWMNERMSRVAGHHVAE 222

Query: 225 FLGSFVANRESKQFSAGGK----------------WLVWKFEGDQTLGDYMKDRSFPVNM 284
           F+ +F  +      +A GK                WLVW +EGD TL   M+ R +P N+
Sbjct: 223 FVTAFDESLNVPLPAAAGKRAAPVQPTSPLDANSIWLVWVYEGDNTLSSLMERREWPYNL 282

Query: 285 EALMFGRVLQGMNSVERNALIIKQILRQIITSLKKIHDTGIVHRDVKPANLVVTKKG-QV 344
           E L+FGR L+      R  + IK+  RQ++ ++   H  GIVHRD+KPAN +V+++  ++
Sbjct: 283 EPLLFGRELRAPRGPVRELVTIKEAFRQLVQAVAACHSVGIVHRDIKPANCIVSERDKKI 342

Query: 345 KIIDFGAATDLRIGKNYVPNLALLDPDYCPPELYVMPEETPSPPPAPIAALLSPILWQLN 404
           K+ID GAA DLRIG NYVPN  LLDP Y PP+ Y+M  +TP PPP P+AA LSPILW + 
Sbjct: 343 KLIDLGAAADLRIGINYVPNEYLLDPRYAPPQQYIMSTQTPKPPPKPVAAFLSPILWTME 402

Query: 405 SPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNLEIKNCGYDLNKWREKTRTK-------- 464
            PD FDMYS GI L+QM   +LR+   L  FN  ++   +DL  WR +   K        
Sbjct: 403 KPDRFDMYSCGITLLQMVFGHLRNDNALIAFNKRLQELKWDLPAWRREEEAKLPSAKGAL 462

Query: 465 -----PDLTILDLDSGRGWDLATKLVSERGFLRRGRLSASAALRHPYFLLGGDQAAAVIS 498
                     LD D G GWDL  +L++   +    R SA+A L HP+      + A++  
Sbjct: 463 AESLEAGFEALDADGGAGWDLLMRLLA---YKPTDRPSAAAVLAHPWLTSAPGRTASLQH 497

BLAST of Spg012522 vs. ExPASy Swiss-Prot
Match: A1A5Q6 (Sperm motility kinase OS=Rattus norvegicus OX=10116 GN=Smok PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.3e-08
Identity = 47/172 (27.33%), Postives = 74/172 (43.02%), Query Frame = 0

Query: 292 QILRQIITSLKKIHDTGIVHRDVKPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALL 351
           ++ RQ++ +L+  H  GI+HRD+KP N+++  KG VK+ DFG +T + +G+        L
Sbjct: 121 RLFRQMVLALQYCHQRGIIHRDIKPENILLDHKGNVKLSDFGLSTKIVMGQKLTTLCGTL 180

Query: 352 DPDYCPPELYVMPEETPSPPPAPIAALLSPILWQLNSPD--LFDMYSAGIVLMQMAVPNL 411
              YC PEL+                        LN  D    D++S G+VL  MA   L
Sbjct: 181 --PYCAPELF-----------------------NLNGYDGQAIDVWSLGVVLYYMATGCL 240

Query: 412 RSSAGLKNFNLEIKNCGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVS 462
                 + F  +         ++    R  PDL          WD+  KL++
Sbjct: 241 ----PFQGFTYQAIKQKILSGRYSVNFRLSPDL----------WDVIAKLLT 253

BLAST of Spg012522 vs. ExPASy Swiss-Prot
Match: Q8W490 (Serine/threonine-protein kinase PEPKR2 OS=Arabidopsis thaliana OX=3702 GN=PEPKR2 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.7e-08
Identity = 62/235 (26.38%), Postives = 97/235 (41.28%), Query Frame = 0

Query: 258 YMKDRSFPVNMEALMFGRVLQGMNSVER-NALIIKQILRQIITSLKKIHDTGIVHRDVKP 317
           Y +   F + ME    GR++  M  V R +      I + ++  +   H+ G+VHRD+KP
Sbjct: 168 YEESDCFHLVMELCSGGRLIDQMVKVGRYSEQRAANIFKDLMLVINYCHEMGVVHRDIKP 227

Query: 318 ANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPELYVMPEETPSPPPAPIA 377
            N+++T  G++++ DFG A  +RI K    +     P Y  PE  V+ E           
Sbjct: 228 ENILLTAAGKIQLADFGLA--MRIAKGQTLSGLAGSPAYVAPE--VLSE----------- 287

Query: 378 ALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNLE-IKNCGYDLNK--WR 437
                     N  +  D++SAG++L  +    L       +   E IKN   D N   W 
Sbjct: 288 ----------NYSEKVDVWSAGVLLYALLSGVLPFKGDSLDAIFEAIKNVKLDFNTGVWE 347

Query: 438 EKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSASAALRHPYFLLGGDQ 489
             ++   DL            LA  L  E       R++A   LRHP+ L   D+
Sbjct: 348 SVSKPARDL------------LARMLTREES----ARITADEVLRHPWILFYTDR 361

BLAST of Spg012522 vs. ExPASy TrEMBL
Match: A0A6J1GDZ5 (serine/threonine-protein kinase STN8, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111453317 PE=4 SV=1)

HSP 1 Score: 907.1 bits (2343), Expect = 3.2e-260
Identity = 453/501 (90.42%), Postives = 476/501 (95.01%), Query Frame = 0

Query: 1   MASLFYPFAISLQQSPQTLCFSPLKPTLQTEIPLFAGAFRAKQPPGKCRASFFGDISDHL 60
           MASL +PFAISLQQ+ QT  FSPLKP L TEIPLFAG FR K P GKCRASFFGDISD L
Sbjct: 1   MASLIFPFAISLQQTHQTR-FSPLKPILPTEIPLFAGTFRPKPPAGKCRASFFGDISDDL 60

Query: 61  IDSLDGPLRVDQLPVLQSGYVQFQRFSGELSDFQKWGFVVFAGIIWVYLTARPGVLFGAI 120
           +++LDGPLRVDQLPVLQSGYVQFQRFSGELSD QKWGF+VFAG IWVYLTARPGVLFGAI
Sbjct: 61  LNNLDGPLRVDQLPVLQSGYVQFQRFSGELSDLQKWGFIVFAGFIWVYLTARPGVLFGAI 120

Query: 121 DTYLLAPLQLGFDSLLGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLRKNVKVEERTSRGL 180
           DTYLLAPLQLG  SL+GRR+LKSSDFVIG KLGEGSFGVVYSGAFL KNVK+E RTS+ L
Sbjct: 121 DTYLLAPLQLGLYSLIGRRNLKSSDFVIGSKLGEGSFGVVYSGAFLPKNVKIENRTSKVL 180

Query: 181 ELDGKEKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVANRESKQFSA 240
           ELDGK+KVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVA+RE++QFSA
Sbjct: 181 ELDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRENRQFSA 240

Query: 241 GGKWLVWKFEGDQTLGDYMKDRSFPVNMEALMFGRVLQGMNSVERNALIIKQILRQIITS 300
           GGKWLVWKFEGDQTLGDYMKDRSFP+N+E LMF RVLQGMNS+ERNALIIKQILRQIITS
Sbjct: 241 GGKWLVWKFEGDQTLGDYMKDRSFPLNLETLMFRRVLQGMNSIERNALIIKQILRQIITS 300

Query: 301 LKKIHDTGIVHRDVKPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL 360
           LKKIHDTGIVHRDVKPANLVVTKKGQ+KIIDFGAATDLRIGKNY+PNLALLDPDYCPPEL
Sbjct: 301 LKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYIPNLALLDPDYCPPEL 360

Query: 361 YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNL 420
           YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVP+LRSSAGLKNFN+
Sbjct: 361 YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPSLRSSAGLKNFNM 420

Query: 421 EIKNCGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSASAALRHP 480
           EIKN GYDLN+WRE+TRTKPDLTILDLDS RGWDLATKLVSERG LRRGRLSASAALRHP
Sbjct: 421 EIKNYGYDLNEWRERTRTKPDLTILDLDSRRGWDLATKLVSERGSLRRGRLSASAALRHP 480

Query: 481 YFLLGGDQAAAVISKLNLVKK 502
           YFLLG DQAAAVISKLNLVKK
Sbjct: 481 YFLLGSDQAAAVISKLNLVKK 500

BLAST of Spg012522 vs. ExPASy TrEMBL
Match: A0A6J1IS67 (serine/threonine-protein kinase STN8, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111478215 PE=4 SV=1)

HSP 1 Score: 903.3 bits (2333), Expect = 4.6e-259
Identity = 453/501 (90.42%), Postives = 474/501 (94.61%), Query Frame = 0

Query: 1   MASLFYPFAISLQQSPQTLCFSPLKPTLQTEIPLFAGAFRAKQPPGKCRASFFGDISDHL 60
           MASL +PFAISLQQ+ QT  FSPLKP L TEIPLFAG FR K   GKCRASFFGDISD L
Sbjct: 1   MASLIFPFAISLQQTHQTR-FSPLKPILPTEIPLFAGTFRPKPSAGKCRASFFGDISDDL 60

Query: 61  IDSLDGPLRVDQLPVLQSGYVQFQRFSGELSDFQKWGFVVFAGIIWVYLTARPGVLFGAI 120
           +++LDGPLR+DQLPVLQSGYVQFQRFSGELSD QKWGF+VFAG IWVYLTARPGVLFGAI
Sbjct: 61  LNNLDGPLRIDQLPVLQSGYVQFQRFSGELSDLQKWGFIVFAGFIWVYLTARPGVLFGAI 120

Query: 121 DTYLLAPLQLGFDSLLGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLRKNVKVEERTSRGL 180
           DTYLLAPLQLG  SL+GRR+LKSSDFVIG KLGEGSFGVVYSGAFL KNVK+E RTS+ L
Sbjct: 121 DTYLLAPLQLGLYSLIGRRNLKSSDFVIGSKLGEGSFGVVYSGAFLPKNVKIENRTSKVL 180

Query: 181 ELDGKEKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVANRESKQFSA 240
           ELDGK+KVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVA+RE++QFSA
Sbjct: 181 ELDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRENRQFSA 240

Query: 241 GGKWLVWKFEGDQTLGDYMKDRSFPVNMEALMFGRVLQGMNSVERNALIIKQILRQIITS 300
           GGKWLVWKFEGDQTLGDYMKDRSFP+N+E LMF RVLQGMNS ERNALIIKQILRQIITS
Sbjct: 241 GGKWLVWKFEGDQTLGDYMKDRSFPLNLETLMFRRVLQGMNSTERNALIIKQILRQIITS 300

Query: 301 LKKIHDTGIVHRDVKPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL 360
           LKKIHDTGIVHRDVKPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL
Sbjct: 301 LKKIHDTGIVHRDVKPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL 360

Query: 361 YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNL 420
           YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVP+LRSSAGLKNFN+
Sbjct: 361 YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPSLRSSAGLKNFNM 420

Query: 421 EIKNCGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSASAALRHP 480
           EIKN GYDLN+WRE+TRTKPDLTILDLDS RGWDLATKLVSERG LRRGRLSASAALRHP
Sbjct: 421 EIKNYGYDLNEWRERTRTKPDLTILDLDSRRGWDLATKLVSERGSLRRGRLSASAALRHP 480

Query: 481 YFLLGGDQAAAVISKLNLVKK 502
           YFLLG DQAAAVISKLNLVKK
Sbjct: 481 YFLLGSDQAAAVISKLNLVKK 500

BLAST of Spg012522 vs. ExPASy TrEMBL
Match: A0A6J1CSB0 (serine/threonine-protein kinase STN8, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111013876 PE=4 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.1e-252
Identity = 444/502 (88.45%), Postives = 469/502 (93.43%), Query Frame = 0

Query: 1   MASLFYPFAISLQQSPQTLCFSPLKPTLQTEIPLFAGAFRAKQPPGKCRASFFGDISDHL 60
           MASL  P +ISLQ +PQTL FSPLK    + + LFAG FR K        +FFGDISD L
Sbjct: 1   MASLLSPLSISLQITPQTLFFSPLK----SHVSLFAGTFRTK--------AFFGDISDRL 60

Query: 61  IDSLDGPLR-VDQLPVLQSGYVQFQRFSGELSDFQKWGFVVFAGIIWVYLTARPGVLFGA 120
           +D+LDGPLR VDQLPVLQSGY+QFQRFSGELSD QKWGF+VFAG++WVYLTARPGVLFGA
Sbjct: 61  LDNLDGPLRPVDQLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGA 120

Query: 121 IDTYLLAPLQLGFDSLLGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLRKNVKVEERTSRG 180
           ID YLLAPLQLG DSL GRRSLKSSDFVIGGKLGEGSFGVVYSGAFL KNVK+EER SRG
Sbjct: 121 IDAYLLAPLQLGLDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRG 180

Query: 181 LELDGKEKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVANRESKQFS 240
           LE+DGK+KVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVA+RES+QFS
Sbjct: 181 LEVDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFS 240

Query: 241 AGGKWLVWKFEGDQTLGDYMKDRSFPVNMEALMFGRVLQGMNSVERNALIIKQILRQIIT 300
           AGGKWLVWKFEGDQTL DYMKDRSFPVN+EALMFGRVL+G+NSVE+NALIIKQILRQII 
Sbjct: 241 AGGKWLVWKFEGDQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIK 300

Query: 301 SLKKIHDTGIVHRDVKPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPE 360
           SLKKIHDTGIVHRDVKPANLVVTKKGQ+KIIDFGAATDLRIGKNYVPNLALLDPDYCPPE
Sbjct: 301 SLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPE 360

Query: 361 LYVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFN 420
           LYVMPEETPSPPPAPIAAL SPI+WQLNSPDLFDMYSAGIVL+QMAVPNLRSSAGLKNFN
Sbjct: 361 LYVMPEETPSPPPAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFN 420

Query: 421 LEIKNCGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSASAALRH 480
           LE+KN GYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSA+AALRH
Sbjct: 421 LELKNYGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRH 480

Query: 481 PYFLLGGDQAAAVISKLNLVKK 502
           PYFLLGGDQAAAVISKLNLVKK
Sbjct: 481 PYFLLGGDQAAAVISKLNLVKK 490

BLAST of Spg012522 vs. ExPASy TrEMBL
Match: A0A0A0L2W9 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G334670 PE=4 SV=1)

HSP 1 Score: 876.7 bits (2264), Expect = 4.6e-251
Identity = 444/501 (88.62%), Postives = 465/501 (92.81%), Query Frame = 0

Query: 1   MASLFYPFAISLQQSPQTLCFSPLKPTLQTEIPLFAGAFRAKQPPGKCRASFFGDISDHL 60
           MASL +PFAISLQQ+      SPLKP+LQT++PL  G F  K   GKCRASFFGDISD+L
Sbjct: 1   MASLIFPFAISLQQTR----LSPLKPSLQTQVPLLPGTFTPKPLAGKCRASFFGDISDNL 60

Query: 61  IDSLDGPLRVDQLPVLQSGYVQFQRFSGELSDFQKWGFVVFAGIIWVYLTARPGVLFGAI 120
           +D+LD PLRVDQLPVLQSGYVQFQRFSGELSD QKWGFVVFAG IWVYLTARPG+L GAI
Sbjct: 61  LDNLDAPLRVDQLPVLQSGYVQFQRFSGELSDLQKWGFVVFAGFIWVYLTARPGILVGAI 120

Query: 121 DTYLLAPLQLGFDSLLGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLRKNVKVEERTSRGL 180
           D YLLAPLQLG DSLLGRRSLK SDFVIGGKLGEGSFGVVY+GAFL KNVK EE+TSR  
Sbjct: 121 DAYLLAPLQLGLDSLLGRRSLKCSDFVIGGKLGEGSFGVVYAGAFLPKNVKNEEQTSRA- 180

Query: 181 ELDGKEKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVANRESKQFSA 240
            LDGKEKVILKKIK+ VKGAE+FGEYEEWFNYRLSRAAPETCA+FLGSFVA+RE+KQFSA
Sbjct: 181 -LDGKEKVILKKIKLRVKGAEEFGEYEEWFNYRLSRAAPETCADFLGSFVASRETKQFSA 240

Query: 241 GGKWLVWKFEGDQTLGDYMKDRSFPVNMEALMFGRVLQGMNSVERNALIIKQILRQIITS 300
           GGKWLVWKFEGDQTLGDYMKDRSFPVN+E+LMFGRVLQ MNSVERNALIIKQILRQIITS
Sbjct: 241 GGKWLVWKFEGDQTLGDYMKDRSFPVNLESLMFGRVLQSMNSVERNALIIKQILRQIITS 300

Query: 301 LKKIHDTGIVHRDVKPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL 360
           LKKIHDTGIVHRDVKPANLVVTKKGQ+KIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL
Sbjct: 301 LKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL 360

Query: 361 YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNL 420
           YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFN+
Sbjct: 361 YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNM 420

Query: 421 EIKNCGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSASAALRHP 480
           EIKN GYDLNKWREKTR KPDLTILDLDSGRGWDLATKLV+E    RRGRLSASAALRHP
Sbjct: 421 EIKNYGYDLNKWREKTRAKPDLTILDLDSGRGWDLATKLVTE----RRGRLSASAALRHP 480

Query: 481 YFLLGGDQAAAVISKLNLVKK 502
           YFLLG DQAAAVISKLNLVKK
Sbjct: 481 YFLLGSDQAAAVISKLNLVKK 491

BLAST of Spg012522 vs. ExPASy TrEMBL
Match: A0A5D3CEV2 (Serine/threonine-protein kinase STN8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold127G00410 PE=4 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 1.9e-249
Identity = 441/501 (88.02%), Postives = 463/501 (92.42%), Query Frame = 0

Query: 1   MASLFYPFAISLQQSPQTLCFSPLKPTLQTEIPLFAGAFRAKQPPGKCRASFFGDISDHL 60
           MASL +PFAISLQQ+      SPLKPTLQT++PL  G F  K   GKCRASFFGDISD+L
Sbjct: 1   MASLIFPFAISLQQTR----LSPLKPTLQTQVPLLPGTFTPKPLAGKCRASFFGDISDNL 60

Query: 61  IDSLDGPLRVDQLPVLQSGYVQFQRFSGELSDFQKWGFVVFAGIIWVYLTARPGVLFGAI 120
           +D+LDGPL+VDQLPV QSGYVQFQRFSG LSD QKWGFVVFAG IWVYLTARPGVL GAI
Sbjct: 61  LDNLDGPLKVDQLPVFQSGYVQFQRFSGGLSDLQKWGFVVFAGFIWVYLTARPGVLVGAI 120

Query: 121 DTYLLAPLQLGFDSLLGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLRKNVKVEERTSRGL 180
           D YLLAPLQLG DSLLGRRSLKSSDFVIGGKLGEGSFGVVY+GAFL KNVK EERTSR  
Sbjct: 121 DAYLLAPLQLGLDSLLGRRSLKSSDFVIGGKLGEGSFGVVYAGAFLPKNVKNEERTSRA- 180

Query: 181 ELDGKEKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVANRESKQFSA 240
            LDGKEKVILKKIK+GVKGAE+ GEYE WFNYRLSRAAPETCA+FLGSFVA+R S+QFSA
Sbjct: 181 -LDGKEKVILKKIKLGVKGAEELGEYEVWFNYRLSRAAPETCADFLGSFVADRASRQFSA 240

Query: 241 GGKWLVWKFEGDQTLGDYMKDRSFPVNMEALMFGRVLQGMNSVERNALIIKQILRQIITS 300
           GGKWLVWKFEGDQTLGDYMKDRSFPVN+E++MFGRVL+GMNSVERNALIIKQILRQIITS
Sbjct: 241 GGKWLVWKFEGDQTLGDYMKDRSFPVNLESVMFGRVLRGMNSVERNALIIKQILRQIITS 300

Query: 301 LKKIHDTGIVHRDVKPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL 360
           LKKIHDTGIVHRDVKPANLVVTKKGQ+KIIDFGAATDLRIGKNYVPN ALLDPDYCPPEL
Sbjct: 301 LKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNRALLDPDYCPPEL 360

Query: 361 YVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNL 420
           YVMPEETPSPPPAPIAALLSPILWQLN+PDLFDMYSAGIVLMQMAVPNLRSSAGLKNFN+
Sbjct: 361 YVMPEETPSPPPAPIAALLSPILWQLNNPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNM 420

Query: 421 EIKNCGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSASAALRHP 480
           EIKN GYDLNKWREKTRTKPDLTILDLDSGRGWDL TKLV+E    RRGRLSASAALRHP
Sbjct: 421 EIKNYGYDLNKWREKTRTKPDLTILDLDSGRGWDLVTKLVTE----RRGRLSASAALRHP 480

Query: 481 YFLLGGDQAAAVISKLNLVKK 502
           YFLLG DQAAAVISKLNLVKK
Sbjct: 481 YFLLGSDQAAAVISKLNLVKK 491

BLAST of Spg012522 vs. TAIR 10
Match: AT5G01920.1 (Protein kinase superfamily protein )

HSP 1 Score: 659.4 bits (1700), Expect = 2.2e-189
Identity = 324/429 (75.52%), Postives = 372/429 (86.71%), Query Frame = 0

Query: 80  YVQFQRFSGELSDFQKWGFVVFAGIIWVYLTARPGVLFGAIDTYLLAPLQLGFDSLLGRR 139
           ++ FQ      SD QKWGF V AGI+W YLTARPGVL GAID YLLAPLQLG D+L+GRR
Sbjct: 68  FLDFQNSLATFSDTQKWGFFVSAGIVWFYLTARPGVLIGAIDAYLLAPLQLGLDTLIGRR 127

Query: 140 SLKSSDFVIGGKLGEGSFGVVYSGAFLRKN------VKVEERTSRGLELDG--KEKVILK 199
            LK SDF++  KLGEGSFGVVY+G  L KN      V+V +  ++ ++  G  K++VILK
Sbjct: 128 -LKRSDFLVTEKLGEGSFGVVYAGVLLPKNSTLVDDVRVSKARAKAMDFTGEFKQRVILK 187

Query: 200 KIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVANRESKQFSAGGKWLVWKFEG 259
           K+KVGV+GAE+FGEYEEWFNYRLSRAAP+TCAEFLGSFVA++ +  F+ GGKWLVW+FEG
Sbjct: 188 KVKVGVRGAEEFGEYEEWFNYRLSRAAPDTCAEFLGSFVADKTNTMFTKGGKWLVWRFEG 247

Query: 260 DQTLGDYMKDRSFPVNMEALMFGRVLQGMNSVERNALIIKQILRQIITSLKKIHDTGIVH 319
           D+ L DYMKDRSFP N+E++MFGRVLQG+ SV+R ALIIKQI+RQIITSL+KIH TGIVH
Sbjct: 248 DRDLADYMKDRSFPSNLESIMFGRVLQGVESVKRRALIIKQIMRQIITSLRKIHGTGIVH 307

Query: 320 RDVKPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPELYVMPEETPSPP 379
           RDVKPANLVVTKKGQ+K+IDFGAA DLRIGKNY+P   LLDPDYCPPELYV+PEETPSPP
Sbjct: 308 RDVKPANLVVTKKGQIKLIDFGAAADLRIGKNYIPERTLLDPDYCPPELYVLPEETPSPP 367

Query: 380 PAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNLEIKNCGYDLNK 439
           P PIAALLSPILWQLNSPDLFDMYSAGIVL+QMAVP LRS+AGLKNFNLEIK+  YDLN+
Sbjct: 368 PEPIAALLSPILWQLNSPDLFDMYSAGIVLLQMAVPTLRSTAGLKNFNLEIKSVEYDLNR 427

Query: 440 WREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSASAALRHPYFLLGGDQAAA 499
           WRE+TRT+PDL+ILDLDSGRGWDL TKL+SERG LRRGRLSA+AALRHPYFLLGGDQAAA
Sbjct: 428 WRERTRTRPDLSILDLDSGRGWDLVTKLISERGSLRRGRLSAAAALRHPYFLLGGDQAAA 487

Query: 500 VISKLNLVK 501
           V+SKL+  K
Sbjct: 488 VLSKLSFSK 495

BLAST of Spg012522 vs. TAIR 10
Match: AT1G68830.1 (STT7 homolog STN7 )

HSP 1 Score: 300.1 bits (767), Expect = 3.4e-81
Identity = 170/398 (42.71%), Postives = 237/398 (59.55%), Query Frame = 0

Query: 108 YLTARPGVLFGAIDTYLLAPLQLGFDSLLGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLR 167
           YL A PGV  G  D ++LA     F   L R + +  DFV+G KLGEGSFGVVY  +  +
Sbjct: 101 YLWATPGVAPGFFDMFVLA-----FVERLFRPTFRKDDFVVGKKLGEGSFGVVYKVSLSK 160

Query: 168 KNVKVEERTSRGLELDGKEKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLG 227
           K    E             + +LKK       A ++G  E W N R+ RA   +CA+F+ 
Sbjct: 161 KRSNEE------------GEYVLKK-------ATEYGAVEIWMNERVRRACGNSCADFVY 220

Query: 228 SFVANRESKQFSAGGKWLVWKFEGDQTLGDYMKDRSFPVNMEALMFGRVLQGMNSVERNA 287
            F+ ++ SK+      WL+WK+EG+ TL   M+ + FP N+E ++ G+V      +ER  
Sbjct: 221 GFL-DKSSKK--GPEYWLLWKYEGESTLAGLMQSKEFPYNVETIILGKVQDLPKGLEREN 280

Query: 288 LIIKQILRQIITSLKKIHDTGIVHRDVKPANLVVTKKGQ-VKIIDFGAATDLRIGKNYVP 347
            II+ I+RQ++ +L  +H TGI+HRDVKP N++ ++  +  KIID GAA DLR+G NY+P
Sbjct: 281 KIIQTIMRQLLFALDGLHSTGIIHRDVKPQNIIFSEGSRSFKIIDLGAAADLRVGINYIP 340

Query: 348 NLALLDPDYCPPELYVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAV 407
              LLDP Y  PE Y+M  +TPS P AP+AA LSP+LWQ+N PD FD+YS G++ +QMA 
Sbjct: 341 KEFLLDPRYAAPEQYIMSTQTPSAPSAPVAAALSPVLWQMNLPDRFDIYSIGLIFLQMAF 400

Query: 408 PNLRSSAGLKNFNLEIKNCGYDLNKWRE--KTRTKPDL----TILDLDSGRGWDLATKLV 467
           P+LRS + L  FN ++K C YDL  WR+  + R   DL     ++DLD G GW+L T +V
Sbjct: 401 PSLRSDSNLIQFNRQLKRCDYDLTAWRKLVEPRASADLRRGFELVDLDGGIGWELLTSMV 460

Query: 468 SERGFLRRGRLSASAALRHPYFLLGGDQAAAVISKLNL 499
               +  R R+SA AAL HPYF   G  A +V+  L +
Sbjct: 461 R---YKARQRISAKAALAHPYFDRQGLLALSVMQNLRM 468

BLAST of Spg012522 vs. TAIR 10
Match: AT1G12680.1 (phosphoenolpyruvate carboxylase-related kinase 2 )

HSP 1 Score: 62.4 bits (150), Expect = 1.2e-09
Identity = 62/235 (26.38%), Postives = 97/235 (41.28%), Query Frame = 0

Query: 258 YMKDRSFPVNMEALMFGRVLQGMNSVER-NALIIKQILRQIITSLKKIHDTGIVHRDVKP 317
           Y +   F + ME    GR++  M  V R +      I + ++  +   H+ G+VHRD+KP
Sbjct: 168 YEESDCFHLVMELCSGGRLIDQMVKVGRYSEQRAANIFKDLMLVINYCHEMGVVHRDIKP 227

Query: 318 ANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPELYVMPEETPSPPPAPIA 377
            N+++T  G++++ DFG A  +RI K    +     P Y  PE  V+ E           
Sbjct: 228 ENILLTAAGKIQLADFGLA--MRIAKGQTLSGLAGSPAYVAPE--VLSE----------- 287

Query: 378 ALLSPILWQLNSPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNLE-IKNCGYDLNK--WR 437
                     N  +  D++SAG++L  +    L       +   E IKN   D N   W 
Sbjct: 288 ----------NYSEKVDVWSAGVLLYALLSGVLPFKGDSLDAIFEAIKNVKLDFNTGVWE 347

Query: 438 EKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSASAALRHPYFLLGGDQ 489
             ++   DL            LA  L  E       R++A   LRHP+ L   D+
Sbjct: 348 SVSKPARDL------------LARMLTREES----ARITADEVLRHPWILFYTDR 361

BLAST of Spg012522 vs. TAIR 10
Match: AT5G63370.1 (Protein kinase superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 3.9e-08
Identity = 89/354 (25.14%), Postives = 155/354 (43.79%), Query Frame = 0

Query: 135 LLGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLRKNVKVEERTSRGLELDGKEKVILKKIK 194
           + G RS+  ++F    K+ EG++G+VY           +E+T        KE V LKKIK
Sbjct: 288 MFGSRSV--NEFQKLNKINEGTYGIVYKAR--------DEKT--------KEIVALKKIK 347

Query: 195 VGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVANRESKQFSAGGKWLVWKFEGDQT 254
           +      K   +EE + + L+ +  E       +  A    K+   GGK        D  
Sbjct: 348 M------KEDRFEEEYGFPLT-SLREINILLSCNHPAIVNVKEVVVGGK-------NDND 407

Query: 255 LGDYMKDRSFPVNMEALMFGRVLQGMNSVERNALIIKQILRQIITSLKKIHDTGIVHRDV 314
           +  YM       ++  +M  R  +  ++ E     +K ++ Q++  LK +H   I+HRD+
Sbjct: 408 V--YMVMEHLEHDLRGVM-DRRKEPFSTSE-----VKCLMMQLLDGLKYLHTNWIIHRDL 467

Query: 315 KPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPD-YCPPELYVMPEE-TPSPPP 374
           KP+NL++   G++KI DFG A   + G    P   ++    Y PPEL +  +E + +   
Sbjct: 468 KPSNLLMNNCGELKICDFGMAR--QYGSPIKPYTQMVITQWYRPPELLLGAKEYSTAVDM 527

Query: 375 APIAALLSPILWQLN-SPDLFDMYSAGIVLMQMAVPNLRSSAGLKNF-NLEIKNCGYDLN 434
             +  +++ +L Q    P   ++     +   +  PN     G  +F N + K      N
Sbjct: 528 WSVGCIMAELLSQKPLFPGKSELDQLQKIFAVLGTPNEAIWPGFSSFPNAKAKFPTQPYN 587

Query: 435 KWREKTRTKPDLTIL--DLDSGRGWDLATKLVSERGFLRRGRLSASAALRHPYF 483
             R+K    P ++ +   + S RG+DL   L++        RL+   AL H +F
Sbjct: 588 MLRKKF---PAISFVGGQILSERGFDLLNSLLT---LDPEKRLTVEDALNHGWF 593

BLAST of Spg012522 vs. TAIR 10
Match: AT5G63370.2 (Protein kinase superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 3.9e-08
Identity = 89/354 (25.14%), Postives = 155/354 (43.79%), Query Frame = 0

Query: 135 LLGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLRKNVKVEERTSRGLELDGKEKVILKKIK 194
           + G RS+  ++F    K+ EG++G+VY           +E+T        KE V LKKIK
Sbjct: 154 MFGSRSV--NEFQKLNKINEGTYGIVYKAR--------DEKT--------KEIVALKKIK 213

Query: 195 VGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVANRESKQFSAGGKWLVWKFEGDQT 254
           +      K   +EE + + L+ +  E       +  A    K+   GGK        D  
Sbjct: 214 M------KEDRFEEEYGFPLT-SLREINILLSCNHPAIVNVKEVVVGGK-------NDND 273

Query: 255 LGDYMKDRSFPVNMEALMFGRVLQGMNSVERNALIIKQILRQIITSLKKIHDTGIVHRDV 314
           +  YM       ++  +M  R  +  ++ E     +K ++ Q++  LK +H   I+HRD+
Sbjct: 274 V--YMVMEHLEHDLRGVM-DRRKEPFSTSE-----VKCLMMQLLDGLKYLHTNWIIHRDL 333

Query: 315 KPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPD-YCPPELYVMPEE-TPSPPP 374
           KP+NL++   G++KI DFG A   + G    P   ++    Y PPEL +  +E + +   
Sbjct: 334 KPSNLLMNNCGELKICDFGMAR--QYGSPIKPYTQMVITQWYRPPELLLGAKEYSTAVDM 393

Query: 375 APIAALLSPILWQLN-SPDLFDMYSAGIVLMQMAVPNLRSSAGLKNF-NLEIKNCGYDLN 434
             +  +++ +L Q    P   ++     +   +  PN     G  +F N + K      N
Sbjct: 394 WSVGCIMAELLSQKPLFPGKSELDQLQKIFAVLGTPNEAIWPGFSSFPNAKAKFPTQPYN 453

Query: 435 KWREKTRTKPDLTIL--DLDSGRGWDLATKLVSERGFLRRGRLSASAALRHPYF 483
             R+K    P ++ +   + S RG+DL   L++        RL+   AL H +F
Sbjct: 454 MLRKKF---PAISFVGGQILSERGFDLLNSLLT---LDPEKRLTVEDALNHGWF 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038881696.13.9e-26092.02serine/threonine-protein kinase STN8, chloroplastic [Benincasa hispida][more]
XP_022950141.16.6e-26090.42serine/threonine-protein kinase STN8, chloroplastic [Cucurbita moschata][more]
XP_023545193.16.6e-26090.42serine/threonine-protein kinase STN8, chloroplastic [Cucurbita pepo subsp. pepo][more]
KAG6603530.11.9e-25990.42Serine/threonine-protein kinase STN8, chloroplastic, partial [Cucurbita argyrosp... [more]
KAG7033713.11.9e-25990.42Serine/threonine-protein kinase STN8, chloroplastic [Cucurbita argyrosperma subs... [more]
Match NameE-valueIdentityDescription
Q9LZV43.1e-18875.52Serine/threonine-protein kinase STN8, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q9S7134.8e-8042.71Serine/threonine-protein kinase STN7, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q84V188.2e-7238.53Serine/threonine-protein kinase stt7, chloroplastic OS=Chlamydomonas reinhardtii... [more]
A1A5Q61.3e-0827.33Sperm motility kinase OS=Rattus norvegicus OX=10116 GN=Smok PE=2 SV=1[more]
Q8W4901.7e-0826.38Serine/threonine-protein kinase PEPKR2 OS=Arabidopsis thaliana OX=3702 GN=PEPKR2... [more]
Match NameE-valueIdentityDescription
A0A6J1GDZ53.2e-26090.42serine/threonine-protein kinase STN8, chloroplastic OS=Cucurbita moschata OX=366... [more]
A0A6J1IS674.6e-25990.42serine/threonine-protein kinase STN8, chloroplastic OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1CSB01.1e-25288.45serine/threonine-protein kinase STN8, chloroplastic OS=Momordica charantia OX=36... [more]
A0A0A0L2W94.6e-25188.62Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G334... [more]
A0A5D3CEV21.9e-24988.02Serine/threonine-protein kinase STN8 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
AT5G01920.12.2e-18975.52Protein kinase superfamily protein [more]
AT1G68830.13.4e-8142.71STT7 homolog STN7 [more]
AT1G12680.11.2e-0926.38phosphoenolpyruvate carboxylase-related kinase 2 [more]
AT5G63370.13.9e-0825.14Protein kinase superfamily protein [more]
AT5G63370.23.9e-0825.14Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 146..482
e-value: 3.1E-11
score: 26.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 288..360
e-value: 1.3E-17
score: 64.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 146..482
score: 23.976223
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 132..249
e-value: 2.9E-22
score: 80.8
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 277..484
e-value: 7.7E-45
score: 155.1
NoneNo IPR availablePIRSRPIRSR037014-2PIRSR037014-2coord: 294..341
e-value: 9.3E-5
score: 18.7
NoneNo IPR availablePANTHERPTHR46699:SF1SERINE/THREONINE-PROTEIN KINASE STN8, CHLOROPLASTICcoord: 1..500
IPR044803Serine/threonine-protein kinase STT7-likePANTHERPTHR46699SERINE/THREONINE-PROTEIN KINASE STN8, CHLOROPLASTIC-RELATEDcoord: 1..500
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 309..321
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 142..483

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg012522.1Spg012522.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0009579 thylakoid
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity