Spg012047 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg012047 vs. NCBI nr
Match: XP_038881533.1 (E3 ubiquitin-protein ligase RGLG2-like [Benincasa hispida] >XP_038881535.1 E3 ubiquitin-protein ligase RGLG2-like [Benincasa hispida])

HSP 1 Score: 724.9 bits (1870), Expect = 3.9e-205
Identity = 352/424 (83.02%), Postives = 381/424 (89.86%), Query Frame = 0

Query: 1   MGGKPSKHIHCHLHIHGANQEQSSKCLCGRNSPPSTSFAPSFRSPSHKINGDDFESLEQV 60
           MGG+PSKHIH H+HIHG NQEQ SKC CG + PPSTS  PS + PS+KINGDDFESLEQV
Sbjct: 1   MGGRPSKHIHTHVHIHGLNQEQPSKCSCGHSPPPSTSSGPSSQYPSYKINGDDFESLEQV 60

Query: 61  TRALEQAGLESSNLIVGIDFTKSNEWTGKESFMGKNLHDLDGACLNPYEQAISIIGKTLQ 120
           T ALE+AGLESSNLI+GIDFTKSNEWTG +SFMG+NLHDL GA LNPYEQAISIIG+TL+
Sbjct: 61  TDALEKAGLESSNLIIGIDFTKSNEWTGTKSFMGRNLHDLAGAFLNPYEQAISIIGRTLE 120

Query: 121 KFDDDNIIPCYGFGDATTGDQEVFSFHPEDRPCNGFEEVLFFYRERVPHVKLAGPTSFAP 180
           KFDDDNIIPCYGFGD TTGD+EVFSFHP+DRPC+GFEEVLF YRERVPHVKLAGPTSFAP
Sbjct: 121 KFDDDNIIPCYGFGDVTTGDREVFSFHPDDRPCHGFEEVLFCYRERVPHVKLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSSGQYHILLIIADGQVTRSIDTPPGHLSPQEQSTVDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTRSIDT PGHLSPQEQST+DAIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSGGQYHILLIIADGQVTRSIDTRPGHLSPQEQSTIDAIVEASKYPLSII 240

Query: 241 LVGVGDGPWDQMYECDDQIPARDFDNFQFVNFTDIMSKGISPSKKETEFALQCLMEIPPQ 300
           LVGVGDGPW QM ECDDQIPARDFDNFQFVNFT+IMSKG SPSKKETEFALQCLMEIPPQ
Sbjct: 241 LVGVGDGPWGQMNECDDQIPARDFDNFQFVNFTEIMSKGTSPSKKETEFALQCLMEIPPQ 300

Query: 301 YRATMSLQLLGQKRKTEGQLPFPLPPPPPMANISLNSSPSYRRTNSNRSAISSFGPSSPS 360
           Y+ TM LQLLGQKR+T+  LPFPLP PPP  N   N SPSYRRT+S+ ++ S  GPSSP+
Sbjct: 301 YKETMRLQLLGQKRETQEHLPFPLPRPPPFINNFRNYSPSYRRTDSDITSASFSGPSSPA 360

Query: 361 HSVFSQSSL-SSSSSFNRICPKCYLNEKDLAFGCGHQTCSDCGQDIIFCPTCQTQITIRI 420
           HS+ S+SS  SSS SFNRICPKCY NEKDLAFGCGHQTCSDCG+D+IFCPTCQTQIT  I
Sbjct: 361 HSILSRSSTWSSSPSFNRICPKCYFNEKDLAFGCGHQTCSDCGKDLIFCPTCQTQITKSI 420

Query: 421 RLRN 424
           +LRN
Sbjct: 421 KLRN 424

BLAST of Spg012047 vs. NCBI nr
Match: XP_008440952.1 (PREDICTED: E3 ubiquitin-protein ligase RGLG2-like [Cucumis melo] >KAA0025590.1 E3 ubiquitin-protein ligase RGLG2-like [Cucumis melo var. makuwa] >TYK12465.1 E3 ubiquitin-protein ligase RGLG2-like [Cucumis melo var. makuwa])

HSP 1 Score: 683.3 bits (1762), Expect = 1.3e-192
Identity = 338/425 (79.53%), Postives = 370/425 (87.06%), Query Frame = 0

Query: 1   MGGKPSKHIHCHLHIHGANQEQSSKCLCGRNSPPSTSFAPSFRSPSHKINGDDFESLEQV 60
           MGGKPSKHIH HLHIH   + Q SKC CG   PPSTS  PSF+ P HKINGDDFESLEQV
Sbjct: 1   MGGKPSKHIHTHLHIHDNTRVQPSKCSCGHTLPPSTSSRPSFQYPRHKINGDDFESLEQV 60

Query: 61  TRALEQAGLESSNLIVGIDFTKSNEWTGKESFMGKNLHDLDGACLNPYEQAISIIGKTLQ 120
           T ALE+AGLESSNLI+GIDFTKSNEWTG +SFMG+NLHDL GA LNPYEQAISIIG+TL+
Sbjct: 61  TEALEKAGLESSNLIIGIDFTKSNEWTGTKSFMGRNLHDLAGAFLNPYEQAISIIGRTLE 120

Query: 121 KFDDDNIIPCYGFGDATTGDQEVFSFHPEDRPCNGFEEVLFFYRERVPHVKLAGPTSFAP 180
           KFDDDN IPCYGFGD TTGD+EVFSFH  DRPC+GFEEVLF YRE VP V LAGPTSFAP
Sbjct: 121 KFDDDNKIPCYGFGDVTTGDREVFSFHSGDRPCHGFEEVLFCYREIVPRVLLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSSGQYHILLIIADGQVTRSIDTPPGHLSPQEQSTVDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTR+IDT  GH SPQEQ+T+DAIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSCGQYHILLIIADGQVTRNIDTRQGHFSPQEQATIDAIVEASKYPLSII 240

Query: 241 LVGVGDGPWDQMYECDDQIPARDFDNFQFVNFTDIMSKGISPSKKETEFALQCLMEIPPQ 300
           LVGVGDGPWDQM ECDD+IPAR FDNFQFVNFT+IMSKG SPSKKETEFALQCLMEIPPQ
Sbjct: 241 LVGVGDGPWDQMNECDDKIPARQFDNFQFVNFTEIMSKGTSPSKKETEFALQCLMEIPPQ 300

Query: 301 YRATMSLQLLGQKRKTEGQLPFPLPPPPPMANISLN-SSPSYRRTNSNRSAISSFGPSSP 360
           Y+ TM LQLLGQK++T+  LPFPLP PPP+ NI  N   PSYRRT+S+ S+ S  G +SP
Sbjct: 301 YKETMRLQLLGQKKETQQHLPFPLPRPPPITNIRRNYYPPSYRRTDSDISS-SFSGSTSP 360

Query: 361 SHSVFSQSSL-SSSSSFNRICPKCYLNEKDLAFGCGHQTCSDCGQDIIFCPTCQTQITIR 420
           +HSV S+SS  SS+ SFNRICPKCYLNEK+LAFGCGHQTCSDCG+D++FCPTCQTQIT  
Sbjct: 361 AHSVLSRSSTWSSTPSFNRICPKCYLNEKELAFGCGHQTCSDCGKDLLFCPTCQTQITKS 420

Query: 421 IRLRN 424
           I+LRN
Sbjct: 421 IKLRN 424

BLAST of Spg012047 vs. NCBI nr
Match: XP_022977935.1 (E3 ubiquitin-protein ligase RGLG5-like [Cucurbita maxima])

HSP 1 Score: 681.8 bits (1758), Expect = 3.8e-192
Identity = 333/424 (78.54%), Postives = 366/424 (86.32%), Query Frame = 0

Query: 1   MGGKPSKHIHCHLHIHGANQEQSSKCLCGRNSPPSTSFAPSFRSPSHKINGDDFESLEQV 60
           MG K SK+IHCH+H +G ++ Q  +C CG + PPST+ APS R PS  INGDDFESLEQV
Sbjct: 1   MGAKSSKNIHCHVHFYGDHRNQPLRCSCGHSLPPSTNSAPSSRPPSPIINGDDFESLEQV 60

Query: 61  TRALEQAGLESSNLIVGIDFTKSNEWTGKESFMGKNLHDLDGACLNPYEQAISIIGKTLQ 120
           + ALE AGLESSNLIVGIDFTKSNEWTGK+SFMG+NLHDLDGACLNPYEQAISIIGKTL+
Sbjct: 61  SEALENAGLESSNLIVGIDFTKSNEWTGKKSFMGRNLHDLDGACLNPYEQAISIIGKTLE 120

Query: 121 KFDDDNIIPCYGFGDATTGDQEVFSFHPEDRPCNGFEEVLFFYRERVPHVKLAGPTSFAP 180
           KFDDDNIIPCYGFGD TTGD+EVFSFHP D PC+GFEEVL  YRE VPHV LAGPTSFAP
Sbjct: 121 KFDDDNIIPCYGFGDVTTGDREVFSFHPGDTPCHGFEEVLHCYRETVPHVNLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSSGQYHILLIIADGQVTRSIDTPPGHLSPQEQSTVDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTRS+DT  GHLS QEQST++AIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSYGQYHILLIIADGQVTRSVDTCQGHLSSQEQSTIEAIVEASKYPLSII 240

Query: 241 LVGVGDGPWDQMYECDDQIPARDFDNFQFVNFTDIMSKGISPSKKETEFALQCLMEIPPQ 300
           LVGVGDGPWDQMY+CDDQIPAR+FDNFQFVNFTDIMSKG  PSKK+TEFALQCLMEIPPQ
Sbjct: 241 LVGVGDGPWDQMYQCDDQIPARNFDNFQFVNFTDIMSKGTPPSKKKTEFALQCLMEIPPQ 300

Query: 301 YRATMSLQLLGQKRKTEGQLPFPLPPPPPMANISLNSSPSYRRTNSNRSAISSFGPSSPS 360
           Y+ATM LQLLGQKR T+G LPFPLP PPP+  IS N  P++RRT S+ S+ S +G SSPS
Sbjct: 301 YKATMRLQLLGQKRNTQGHLPFPLPRPPPITYISRNHFPNFRRTESDISSTSFYGSSSPS 360

Query: 361 HSVFSQSS--LSSSSSFNRICPKCYLNEKDLAFGCGHQTCSDCGQDIIFCPTCQTQITIR 420
           HS   +SS   S SSSFNRIC +CY+NEKDLA GCGHQ CS CG+D+I CP CQT ITIR
Sbjct: 361 HSALLRSSSVSSPSSSFNRICSQCYINEKDLALGCGHQICSACGEDLIACPICQTHITIR 420

Query: 421 IRLR 423
           IRLR
Sbjct: 421 IRLR 424

BLAST of Spg012047 vs. NCBI nr
Match: XP_023544775.1 (E3 ubiquitin-protein ligase RGLG5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 681.0 bits (1756), Expect = 6.5e-192
Identity = 335/435 (77.01%), Postives = 369/435 (84.83%), Query Frame = 0

Query: 1   MGGKPSKHIHCHLHIHGANQEQSSKCLCGRNSP-----------PSTSFAPSFRSPSHKI 60
           MG K SK+IHCH+H++G ++ Q  +C CG + P           PST+ APS R P+  I
Sbjct: 1   MGAKSSKNIHCHVHLYGDHRNQPLRCSCGYSPPPTTSPWPSSPSPSTNSAPSSRPPNPII 60

Query: 61  NGDDFESLEQVTRALEQAGLESSNLIVGIDFTKSNEWTGKESFMGKNLHDLDGACLNPYE 120
           NGDDFESLEQV+ ALE+AGLESSNLIVGIDFTKSNEWTGK+SFMG+NLHDLDGACLNPYE
Sbjct: 61  NGDDFESLEQVSEALEKAGLESSNLIVGIDFTKSNEWTGKKSFMGRNLHDLDGACLNPYE 120

Query: 121 QAISIIGKTLQKFDDDNIIPCYGFGDATTGDQEVFSFHPEDRPCNGFEEVLFFYRERVPH 180
           QAISIIGKTL+KFDDDNIIPCYGFGD TTGD++VFSFHP D PC+GFEEVL  YRE VPH
Sbjct: 121 QAISIIGKTLEKFDDDNIIPCYGFGDVTTGDRDVFSFHPGDTPCHGFEEVLHCYRETVPH 180

Query: 181 VKLAGPTSFAPIIRNAIRIVNDSSGQYHILLIIADGQVTRSIDTPPGHLSPQEQSTVDAI 240
           VKLAGPTSFAPIIRNAIRIVNDS GQYHILLIIADGQVTRS+DT  GHLSPQEQST++AI
Sbjct: 181 VKLAGPTSFAPIIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTCQGHLSPQEQSTIEAI 240

Query: 241 VEASKYPLSIILVGVGDGPWDQMYECDDQIPARDFDNFQFVNFTDIMSKGISPSKKETEF 300
           VEASKYPLSIILVGVGDGPWDQMY CDDQIPARDFDNFQFVNFTDIMSKG  PSKK+TEF
Sbjct: 241 VEASKYPLSIILVGVGDGPWDQMYRCDDQIPARDFDNFQFVNFTDIMSKGTPPSKKKTEF 300

Query: 301 ALQCLMEIPPQYRATMSLQLLGQKRKTEGQLPFPLPPPPPMANISLNSSPSYRRTNSNRS 360
           ALQCLMEIPPQY+ATM LQLLGQKR T+G LPFPLP PPP+  IS N  P++RRT S+ S
Sbjct: 301 ALQCLMEIPPQYKATMRLQLLGQKRNTQGHLPFPLPRPPPITYISRNHFPNFRRTESDIS 360

Query: 361 AISSFGPSSPSHSVFSQSS--LSSSSSFNRICPKCYLNEKDLAFGCGHQTCSDCGQDIIF 420
           + S +G SSPSHS   +SS   S SSSFNRIC KCY+NEKDLAFGCGHQ CS CG+D+I 
Sbjct: 361 STSFYGSSSPSHSALLRSSSVSSPSSSFNRICSKCYINEKDLAFGCGHQICSVCGEDLIL 420

Query: 421 CPTCQTQITIRIRLR 423
           CP CQT IT RIRLR
Sbjct: 421 CPICQTHITTRIRLR 435

BLAST of Spg012047 vs. NCBI nr
Match: XP_022950531.1 (E3 ubiquitin-protein ligase RGLG5-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 677.6 bits (1747), Expect = 7.2e-191
Identity = 330/424 (77.83%), Postives = 366/424 (86.32%), Query Frame = 0

Query: 1   MGGKPSKHIHCHLHIHGANQEQSSKCLCGRNSPPSTSFAPSFRSPSHKINGDDFESLEQV 60
           MG K SK+IHCH+HI+G ++ Q  +C CG + PPST+ A S R P+  INGDDFESLEQV
Sbjct: 1   MGAKSSKNIHCHVHIYGDHRNQPLRCSCGYSPPPSTNSARSSRPPNPIINGDDFESLEQV 60

Query: 61  TRALEQAGLESSNLIVGIDFTKSNEWTGKESFMGKNLHDLDGACLNPYEQAISIIGKTLQ 120
           + ALE+AGLESSNLIVGIDFT+SNEWTGK+SFMG+NLHDLDGACLNPYEQAISIIGKTL+
Sbjct: 61  SEALEKAGLESSNLIVGIDFTRSNEWTGKKSFMGRNLHDLDGACLNPYEQAISIIGKTLE 120

Query: 121 KFDDDNIIPCYGFGDATTGDQEVFSFHPEDRPCNGFEEVLFFYRERVPHVKLAGPTSFAP 180
           KFDDDNIIPCYGFGD TTGD++VFSFHP D PC+GFEEVL  YRE VPHV LAGPTSFAP
Sbjct: 121 KFDDDNIIPCYGFGDVTTGDRDVFSFHPGDTPCHGFEEVLHCYRETVPHVNLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSSGQYHILLIIADGQVTRSIDTPPGHLSPQEQSTVDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTRS+DT  GHLSPQEQST++AIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSYGQYHILLIIADGQVTRSVDTCQGHLSPQEQSTIEAIVEASKYPLSII 240

Query: 241 LVGVGDGPWDQMYECDDQIPARDFDNFQFVNFTDIMSKGISPSKKETEFALQCLMEIPPQ 300
           LVGVGDGPWDQMY+CDDQIPARDFDNFQFVNFTDIMSKG  PSKK+TEFALQCLMEIPPQ
Sbjct: 241 LVGVGDGPWDQMYQCDDQIPARDFDNFQFVNFTDIMSKGTPPSKKKTEFALQCLMEIPPQ 300

Query: 301 YRATMSLQLLGQKRKTEGQLPFPLPPPPPMANISLNSSPSYRRTNSNRSAISSFGPSSPS 360
           Y+ATM LQLLGQKR T+G LPFPLP PPP+  IS N  P++RRT S+ S+ S +G SSPS
Sbjct: 301 YKATMRLQLLGQKRNTQGHLPFPLPRPPPITYISRNHFPNFRRTESDISSTSFYGSSSPS 360

Query: 361 HSVFSQSS--LSSSSSFNRICPKCYLNEKDLAFGCGHQTCSDCGQDIIFCPTCQTQITIR 420
           HS   +SS   S S SFNRIC +CY+NEKDLA GCGHQ CS CG+D+I CP CQT IT R
Sbjct: 361 HSALLRSSSVSSPSLSFNRICSQCYINEKDLALGCGHQICSVCGEDLILCPICQTHITTR 420

Query: 421 IRLR 423
           IRLR
Sbjct: 421 IRLR 424

BLAST of Spg012047 vs. ExPASy Swiss-Prot
Match: Q9LY87 (E3 ubiquitin-protein ligase RGLG2 OS=Arabidopsis thaliana OX=3702 GN=RGLG2 PE=1 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 4.2e-125
Identity = 241/401 (60.10%), Postives = 284/401 (70.82%), Query Frame = 0

Query: 31  NSPPSTSFAPSFR---SPSHKINGDDFESLEQVTRALEQAGLESSNLIVGIDFTKSNEWT 90
           ++PPS S+    +      +    DD+ SLEQVT AL +AGLESSNLIVGIDFTKSNEWT
Sbjct: 77  SAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLIVGIDFTKSNEWT 136

Query: 91  GKESFMGKNLHDLDGACLNPYEQAISIIGKTLQKFDDDNIIPCYGFGDATTGDQEVFSFH 150
           G  SF  K+LH + G+  NPYEQAI+IIG+TL  FD+DN+IPCYGFGDA+T DQ+VFSF+
Sbjct: 137 GARSFNRKSLHFI-GSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFN 196

Query: 151 PEDRPCNGFEEVLFFYRERVPHVKLAGPTSFAPIIRNAIRIVNDSSGQYHILLIIADGQV 210
            EDR CNGFEEVL  Y+E VP +KLAGPTSFAPII  A+ IV  S GQYH+L+IIADGQV
Sbjct: 197 SEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVIIADGQV 256

Query: 211 TRSIDTPPGHLSPQEQSTVDAIVEASKYPLSIILVGVGDGPWDQMYECDDQIPARDFDNF 270
           TRS+DT  G LSPQEQ TVDAIV+ASK PLSI+LVGVGDGPWD M E DD IPAR FDNF
Sbjct: 257 TRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPARAFDNF 316

Query: 271 QFVNFTDIMSKGISPSKKETEFALQCLMEIPPQYRATMSLQLLGQKRKTEGQLPFPLPPP 330
           QFVNFT+IM+K  + S KETEFAL  LMEIP QY+AT+ L LLG++    G +P   P P
Sbjct: 317 QFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGRR---NGYIPERFPLP 376

Query: 331 PPMANISLNSSPSYRRTNSNRSAISSFGPSSPSHSVFSQSSLS-------SSSSFNRICP 390
           PPM       S SY     +R  + SF PS P H        S       SS+S N++CP
Sbjct: 377 PPMR----GGSSSYNSPKPSR--LPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCP 436

Query: 391 KCYLNEKDLAFGCGHQTCSDCGQDIIFCPTCQTQITIRIRL 422
            C  N KD+AFGCGHQTC +CG D+  CP C+  I  RI+L
Sbjct: 437 ICLSNPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKL 467

BLAST of Spg012047 vs. ExPASy Swiss-Prot
Match: Q9SS90 (E3 ubiquitin-protein ligase RGLG1 OS=Arabidopsis thaliana OX=3702 GN=RGLG1 PE=1 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.5e-122
Identity = 241/411 (58.64%), Postives = 287/411 (69.83%), Query Frame = 0

Query: 16  HGANQEQSSKCLCGRNSPPSTSFA-----PSFRSPSHKINGDDFESLEQVTRALEQAGLE 75
           H  +QE  +      N PP  S +       F     KI+ D++ SL QV+ AL +AGLE
Sbjct: 95  HQYSQEGYASAASQPNYPPPPSQSQVADRKKFDRRYSKIS-DNYSSLLQVSEALGRAGLE 154

Query: 76  SSNLIVGIDFTKSNEWTGKESFMGKNLHDLDGACLNPYEQAISIIGKTLQKFDDDNIIPC 135
           SSNLIVGIDFTKSNEWTG +SF  K+LH L     NPYEQAI+IIG+TL  FD+DN+IPC
Sbjct: 155 SSNLIVGIDFTKSNEWTGAKSFNRKSLHHLSNT-PNPYEQAITIIGRTLAAFDEDNLIPC 214

Query: 136 YGFGDATTGDQEVFSFHPEDRPCNGFEEVLFFYRERVPHVKLAGPTSFAPIIRNAIRIVN 195
           YGFGDA+T DQ+VFSF+PE R CNGFEEVL  YRE VP +KLAGPTSFAPII  A+ +V 
Sbjct: 215 YGFGDASTHDQDVFSFYPEGRFCNGFEEVLARYREIVPQLKLAGPTSFAPIIEMAMTVVE 274

Query: 196 DSSGQYHILLIIADGQVTRSIDTPPGHLSPQEQSTVDAIVEASKYPLSIILVGVGDGPWD 255
            SSGQYH+L+IIADGQVTRS+DT  G LSPQEQ TVDAIV+AS  PLSI+LVGVGDGPWD
Sbjct: 275 QSSGQYHVLVIIADGQVTRSVDTEHGRLSPQEQKTVDAIVKASTLPLSIVLVGVGDGPWD 334

Query: 256 QMYECDDQIPARDFDNFQFVNFTDIMSKGISPSKKETEFALQCLMEIPPQYRATMSLQLL 315
            M E DD IPAR FDNFQFVNFT+IMSK    S+KETEFAL  LMEIPPQY+AT+ L LL
Sbjct: 335 MMQEFDDNIPARAFDNFQFVNFTEIMSKNKDQSRKETEFALSALMEIPPQYKATIELNLL 394

Query: 316 GQKRKTEGQLPFPLPPPPPMANISLNSSPSYRRTNSNRSAISSFGPSSPSHSVFSQSSLS 375
           G +    G +P  +P PPP+ + S  SS          S I +F PS P +  F    +S
Sbjct: 395 GVR---NGNIPQRIPLPPPVQSGSSFSS----------SRIPNFEPSVPPYP-FESKQMS 454

Query: 376 SSSSFNRICPKCYLNEKDLAFGCGHQTCSDCGQDIIFCPTCQTQITIRIRL 422
           S+    ++CP C  N K++AFGCGHQTC +CG D+  CP C+  I  RI+L
Sbjct: 455 SADDI-QLCPICLSNPKNMAFGCGHQTCCECGPDLKVCPICRAPIQTRIKL 488

BLAST of Spg012047 vs. ExPASy Swiss-Prot
Match: Q8LB88 (E3 ubiquitin-protein ligase RGLG5 OS=Arabidopsis thaliana OX=3702 GN=RGLG5 PE=1 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 4.3e-122
Identity = 224/371 (60.38%), Postives = 274/371 (73.85%), Query Frame = 0

Query: 52  DDFESLEQVTRALEQAGLESSNLIVGIDFTKSNEWTGKESFMGKNLHDLDGACLNPYEQA 111
           D++ S+++VT AL  AGLESSNLIVGID TKSNEWTG  SF  K+LH + G   NPY+QA
Sbjct: 72  DNYRSIDEVTAALSHAGLESSNLIVGIDVTKSNEWTGARSFGRKSLHFI-GTTPNPYQQA 131

Query: 112 ISIIGKTLQKFDDDNIIPCYGFGDATTGDQEVFSFHPEDRPCNGFEEVLFFYRERVPHVK 171
           ISIIGKTL  FD+DN+IPCYGFGDATT DQ+VFSF+P D  CNGFEEVL  YRE VP ++
Sbjct: 132 ISIIGKTLSVFDEDNLIPCYGFGDATTHDQDVFSFNPNDTYCNGFEEVLMCYREIVPQLR 191

Query: 172 LAGPTSFAPIIRNAIRIVNDSSGQYHILLIIADGQVTRSIDTPPGHLSPQEQSTVDAIVE 231
           L+GPTSFAPII  A+ IV +S GQYH+LLIIADGQVTRS+DT  G  SPQEQ T+DAIV 
Sbjct: 192 LSGPTSFAPIIERAMTIVEESGGQYHVLLIIADGQVTRSVDTDNGGFSPQEQQTIDAIVR 251

Query: 232 ASKYPLSIILVGVGDGPWDQMYECDDQIPARDFDNFQFVNFTDIMSKGISPSKKETEFAL 291
           AS+YPLSI+LVGVGDGPWD M + DD IPAR FDNFQFVNFTDIMSK I P++KE EFAL
Sbjct: 252 ASEYPLSIVLVGVGDGPWDTMRQFDDNIPARAFDNFQFVNFTDIMSKNIDPARKEAEFAL 311

Query: 292 QCLMEIPPQYRATMSLQLLGQKRKTEGQLPFPLP-PPPPMANISLNSSPSYRRTNSNRSA 351
             LMEIP QY+AT+ L LLGQ+    G  P  +  PPP  A  S+ +SP   R+ S ++ 
Sbjct: 312 SALMEIPSQYKATLELGLLGQR---TGHCPDRIALPPPTYATQSMRNSPRTSRSTSFQNK 371

Query: 352 ISSFGPSSPSHSVFSQSSLSSSSSFNRICPKCYLNEKDLAFGCGHQTCSDCGQDIIFCPT 411
               G SS      +  S + + S  + CP C ++ K++AF CGHQTC+ CG+D+  CP 
Sbjct: 372 PYDNGVSS------TPPSTTHNESQQQFCPVCLVSAKNMAFNCGHQTCAGCGEDLHVCPI 431

Query: 412 CQTQITIRIRL 422
           C++ I++RI+L
Sbjct: 432 CRSSISVRIKL 432

BLAST of Spg012047 vs. ExPASy Swiss-Prot
Match: Q9SAL0 (E3 ubiquitin-protein ligase RGLG4 OS=Arabidopsis thaliana OX=3702 GN=RGLG4 PE=1 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 3.9e-107
Identity = 203/395 (51.39%), Postives = 264/395 (66.84%), Query Frame = 0

Query: 32  SPPSTSFAPSFRSPSHK--INGDDFESLEQVTRALEQAGLESSNLIVGIDFTKSNEWTGK 91
           S PS S A +  +   K  +  D F SL+QV++AL +AGLESSNLI+G+DFTKSNEWTGK
Sbjct: 36  SDPSLSLADNTNATKKKYALIPDRFSSLDQVSKALREAGLESSNLILGVDFTKSNEWTGK 95

Query: 92  ESFMGKNLHDLDGACLNPYEQAISIIGKTLQKFDDDNIIPCYGFGDATTGDQEVFSFHPE 151
            SF GK LH L G   NPYE+AI +IG+TL  FD+DN+IPC+GFGD+TT D+EVF FH +
Sbjct: 96  TSFDGKCLHAL-GETSNPYEKAIFVIGQTLAPFDEDNLIPCFGFGDSTTHDEEVFGFHSD 155

Query: 152 DRPCNGFEEVLFFYRERVPHVKLAGPTSFAPIIRNAIRIVNDSSGQYHILLIIADGQVTR 211
           + PC+GFEEVL  Y+   P+++L+GPTS+ P+I  A+ IV  ++GQ+H+L+I+ADGQVTR
Sbjct: 156 NSPCHGFEEVLACYKRIAPNLRLSGPTSYGPLIDAAVDIVEKNNGQFHVLVIVADGQVTR 215

Query: 212 SIDTPPGHLSPQEQSTVDAIVEASKYPLSIILVGVGDGPWDQMYECDDQIPARDFDNFQF 271
             D   G LS QE++T+DAIV AS Y LSI+LVGVGDGPW+ M + DD+IP R+FDNFQF
Sbjct: 216 GTDMAEGELSQQEKTTIDAIVNASSYALSIVLVGVGDGPWEDMRKFDDKIPKREFDNFQF 275

Query: 272 VNFTDIMSKGISPSKKETEFALQCLMEIPPQYRATMSLQLLGQK---RKTEGQLPFPLPP 331
           VNFT+IM++    S KET FAL  LMEIP QY+A + L+LLG++    KT    P P+P 
Sbjct: 276 VNFTEIMTRNSPESAKETAFALAALMEIPFQYQAAIELRLLGKQTGLAKTIVPRPPPIPY 335

Query: 332 PPPMANISLNSSPSYRRTNSNRSAISSFGPSSPSHSVFSQSSLSSSSSFNRICPKCYLNE 391
            PP                +N    S+  P+SP  +              + CP C  N 
Sbjct: 336 TPP----------------TNAELPSTASPASPEQT--------------QSCPICLTNR 395

Query: 392 KDLAFGCGHQTCSDCGQDIIFCPTCQTQITIRIRL 422
           KD+AF CGH TC DCG  I  CP C+ +IT R++L
Sbjct: 396 KDVAFSCGHMTCGDCGSKISNCPICRVRITNRLKL 399

BLAST of Spg012047 vs. ExPASy Swiss-Prot
Match: Q8RX26 (E3 ubiquitin-protein ligase RGLG3 OS=Arabidopsis thaliana OX=3702 GN=RGLG3 PE=1 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 1.5e-106
Identity = 204/370 (55.14%), Postives = 257/370 (69.46%), Query Frame = 0

Query: 52  DDFESLEQVTRALEQAGLESSNLIVGIDFTKSNEWTGKESFMGKNLHDLDGACLNPYEQA 111
           D F SL+QV  +L +AGLESSNLI+GIDFTKSNEWTG+ SF  K+LH + G   NPYE+A
Sbjct: 16  DHFSSLDQVITSLREAGLESSNLILGIDFTKSNEWTGRYSFNRKSLHAI-GKRQNPYEKA 75

Query: 112 ISIIGKTLQKFDDDNIIPCYGFGDATTGDQEVFSFHPEDRPCNGFEEVLFFYRERVPHVK 171
           ISIIG+TL  FD+D++IPC+GFGD TT DQ VFSF+PE++ C+G E  +  YRE VPH+K
Sbjct: 76  ISIIGRTLSPFDEDDLIPCFGFGDVTTRDQYVFSFYPENKSCDGLENAVKRYREIVPHLK 135

Query: 172 LAGPTSFAPIIRNAIRIVNDSSGQYHILLIIADGQVTRSIDTPPGHLSPQEQSTVDAIVE 231
           L+GPTSFAP+I  AI IV  ++ QYH+L+IIADGQVTR+ D P G LSPQE++T+++I+ 
Sbjct: 136 LSGPTSFAPVIDAAINIVEQNNMQYHVLVIIADGQVTRNPDVPLGRLSPQEEATMNSIMA 195

Query: 232 ASKYPLSIILVGVGDGPWDQMYECDDQIPARDFDNFQFVNFTDIMSKGISPSKKETEFAL 291
           AS YPLSI+LVGVGDGPWD M + DD IP R+FDNFQFVNFT IMS+    +KKE  FAL
Sbjct: 196 ASHYPLSIVLVGVGDGPWDTMKQFDDNIPHREFDNFQFVNFTKIMSEHKDAAKKEAAFAL 255

Query: 292 QCLMEIPPQYRATMSLQLLGQKRKTEGQLPFPLPPPPPMANISLNSSPSYRRTNSNRSAI 351
             LMEIP QY+AT+SL    +  ++  Q   PLPPPP +            R N+ RS  
Sbjct: 256 AALMEIPFQYKATLSLN--RKPVRSSHQHHKPLPPPPEV----------IERDNAVRSV- 315

Query: 352 SSFGPSSPSHSVFSQSSLSSSSSFNRICPKCYLNEKDLAFGCGHQTCSDCGQDIIFCPTC 411
               P+  + +      L+ S+    +CP C  N KD+AF CGH TC +CG  I  CP C
Sbjct: 316 ----PNQMTETAEKSDRLAPSTV--PVCPICLTNPKDMAFSCGHTTCKECGVVITTCPLC 365

Query: 412 QTQITIRIRL 422
           +  IT RIRL
Sbjct: 376 RQPITTRIRL 365

BLAST of Spg012047 vs. ExPASy TrEMBL
Match: A0A5D3CLM2 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00410 PE=4 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 6.3e-193
Identity = 338/425 (79.53%), Postives = 370/425 (87.06%), Query Frame = 0

Query: 1   MGGKPSKHIHCHLHIHGANQEQSSKCLCGRNSPPSTSFAPSFRSPSHKINGDDFESLEQV 60
           MGGKPSKHIH HLHIH   + Q SKC CG   PPSTS  PSF+ P HKINGDDFESLEQV
Sbjct: 1   MGGKPSKHIHTHLHIHDNTRVQPSKCSCGHTLPPSTSSRPSFQYPRHKINGDDFESLEQV 60

Query: 61  TRALEQAGLESSNLIVGIDFTKSNEWTGKESFMGKNLHDLDGACLNPYEQAISIIGKTLQ 120
           T ALE+AGLESSNLI+GIDFTKSNEWTG +SFMG+NLHDL GA LNPYEQAISIIG+TL+
Sbjct: 61  TEALEKAGLESSNLIIGIDFTKSNEWTGTKSFMGRNLHDLAGAFLNPYEQAISIIGRTLE 120

Query: 121 KFDDDNIIPCYGFGDATTGDQEVFSFHPEDRPCNGFEEVLFFYRERVPHVKLAGPTSFAP 180
           KFDDDN IPCYGFGD TTGD+EVFSFH  DRPC+GFEEVLF YRE VP V LAGPTSFAP
Sbjct: 121 KFDDDNKIPCYGFGDVTTGDREVFSFHSGDRPCHGFEEVLFCYREIVPRVLLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSSGQYHILLIIADGQVTRSIDTPPGHLSPQEQSTVDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTR+IDT  GH SPQEQ+T+DAIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSCGQYHILLIIADGQVTRNIDTRQGHFSPQEQATIDAIVEASKYPLSII 240

Query: 241 LVGVGDGPWDQMYECDDQIPARDFDNFQFVNFTDIMSKGISPSKKETEFALQCLMEIPPQ 300
           LVGVGDGPWDQM ECDD+IPAR FDNFQFVNFT+IMSKG SPSKKETEFALQCLMEIPPQ
Sbjct: 241 LVGVGDGPWDQMNECDDKIPARQFDNFQFVNFTEIMSKGTSPSKKETEFALQCLMEIPPQ 300

Query: 301 YRATMSLQLLGQKRKTEGQLPFPLPPPPPMANISLN-SSPSYRRTNSNRSAISSFGPSSP 360
           Y+ TM LQLLGQK++T+  LPFPLP PPP+ NI  N   PSYRRT+S+ S+ S  G +SP
Sbjct: 301 YKETMRLQLLGQKKETQQHLPFPLPRPPPITNIRRNYYPPSYRRTDSDISS-SFSGSTSP 360

Query: 361 SHSVFSQSSL-SSSSSFNRICPKCYLNEKDLAFGCGHQTCSDCGQDIIFCPTCQTQITIR 420
           +HSV S+SS  SS+ SFNRICPKCYLNEK+LAFGCGHQTCSDCG+D++FCPTCQTQIT  
Sbjct: 361 AHSVLSRSSTWSSTPSFNRICPKCYLNEKELAFGCGHQTCSDCGKDLLFCPTCQTQITKS 420

Query: 421 IRLRN 424
           I+LRN
Sbjct: 421 IKLRN 424

BLAST of Spg012047 vs. ExPASy TrEMBL
Match: A0A1S3B2Y1 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucumis melo OX=3656 GN=LOC103485213 PE=4 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 6.3e-193
Identity = 338/425 (79.53%), Postives = 370/425 (87.06%), Query Frame = 0

Query: 1   MGGKPSKHIHCHLHIHGANQEQSSKCLCGRNSPPSTSFAPSFRSPSHKINGDDFESLEQV 60
           MGGKPSKHIH HLHIH   + Q SKC CG   PPSTS  PSF+ P HKINGDDFESLEQV
Sbjct: 1   MGGKPSKHIHTHLHIHDNTRVQPSKCSCGHTLPPSTSSRPSFQYPRHKINGDDFESLEQV 60

Query: 61  TRALEQAGLESSNLIVGIDFTKSNEWTGKESFMGKNLHDLDGACLNPYEQAISIIGKTLQ 120
           T ALE+AGLESSNLI+GIDFTKSNEWTG +SFMG+NLHDL GA LNPYEQAISIIG+TL+
Sbjct: 61  TEALEKAGLESSNLIIGIDFTKSNEWTGTKSFMGRNLHDLAGAFLNPYEQAISIIGRTLE 120

Query: 121 KFDDDNIIPCYGFGDATTGDQEVFSFHPEDRPCNGFEEVLFFYRERVPHVKLAGPTSFAP 180
           KFDDDN IPCYGFGD TTGD+EVFSFH  DRPC+GFEEVLF YRE VP V LAGPTSFAP
Sbjct: 121 KFDDDNKIPCYGFGDVTTGDREVFSFHSGDRPCHGFEEVLFCYREIVPRVLLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSSGQYHILLIIADGQVTRSIDTPPGHLSPQEQSTVDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTR+IDT  GH SPQEQ+T+DAIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSCGQYHILLIIADGQVTRNIDTRQGHFSPQEQATIDAIVEASKYPLSII 240

Query: 241 LVGVGDGPWDQMYECDDQIPARDFDNFQFVNFTDIMSKGISPSKKETEFALQCLMEIPPQ 300
           LVGVGDGPWDQM ECDD+IPAR FDNFQFVNFT+IMSKG SPSKKETEFALQCLMEIPPQ
Sbjct: 241 LVGVGDGPWDQMNECDDKIPARQFDNFQFVNFTEIMSKGTSPSKKETEFALQCLMEIPPQ 300

Query: 301 YRATMSLQLLGQKRKTEGQLPFPLPPPPPMANISLN-SSPSYRRTNSNRSAISSFGPSSP 360
           Y+ TM LQLLGQK++T+  LPFPLP PPP+ NI  N   PSYRRT+S+ S+ S  G +SP
Sbjct: 301 YKETMRLQLLGQKKETQQHLPFPLPRPPPITNIRRNYYPPSYRRTDSDISS-SFSGSTSP 360

Query: 361 SHSVFSQSSL-SSSSSFNRICPKCYLNEKDLAFGCGHQTCSDCGQDIIFCPTCQTQITIR 420
           +HSV S+SS  SS+ SFNRICPKCYLNEK+LAFGCGHQTCSDCG+D++FCPTCQTQIT  
Sbjct: 361 AHSVLSRSSTWSSTPSFNRICPKCYLNEKELAFGCGHQTCSDCGKDLLFCPTCQTQITKS 420

Query: 421 IRLRN 424
           I+LRN
Sbjct: 421 IKLRN 424

BLAST of Spg012047 vs. ExPASy TrEMBL
Match: A0A6J1INP4 (E3 ubiquitin-protein ligase RGLG5-like OS=Cucurbita maxima OX=3661 GN=LOC111478078 PE=4 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.8e-192
Identity = 333/424 (78.54%), Postives = 366/424 (86.32%), Query Frame = 0

Query: 1   MGGKPSKHIHCHLHIHGANQEQSSKCLCGRNSPPSTSFAPSFRSPSHKINGDDFESLEQV 60
           MG K SK+IHCH+H +G ++ Q  +C CG + PPST+ APS R PS  INGDDFESLEQV
Sbjct: 1   MGAKSSKNIHCHVHFYGDHRNQPLRCSCGHSLPPSTNSAPSSRPPSPIINGDDFESLEQV 60

Query: 61  TRALEQAGLESSNLIVGIDFTKSNEWTGKESFMGKNLHDLDGACLNPYEQAISIIGKTLQ 120
           + ALE AGLESSNLIVGIDFTKSNEWTGK+SFMG+NLHDLDGACLNPYEQAISIIGKTL+
Sbjct: 61  SEALENAGLESSNLIVGIDFTKSNEWTGKKSFMGRNLHDLDGACLNPYEQAISIIGKTLE 120

Query: 121 KFDDDNIIPCYGFGDATTGDQEVFSFHPEDRPCNGFEEVLFFYRERVPHVKLAGPTSFAP 180
           KFDDDNIIPCYGFGD TTGD+EVFSFHP D PC+GFEEVL  YRE VPHV LAGPTSFAP
Sbjct: 121 KFDDDNIIPCYGFGDVTTGDREVFSFHPGDTPCHGFEEVLHCYRETVPHVNLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSSGQYHILLIIADGQVTRSIDTPPGHLSPQEQSTVDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTRS+DT  GHLS QEQST++AIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSYGQYHILLIIADGQVTRSVDTCQGHLSSQEQSTIEAIVEASKYPLSII 240

Query: 241 LVGVGDGPWDQMYECDDQIPARDFDNFQFVNFTDIMSKGISPSKKETEFALQCLMEIPPQ 300
           LVGVGDGPWDQMY+CDDQIPAR+FDNFQFVNFTDIMSKG  PSKK+TEFALQCLMEIPPQ
Sbjct: 241 LVGVGDGPWDQMYQCDDQIPARNFDNFQFVNFTDIMSKGTPPSKKKTEFALQCLMEIPPQ 300

Query: 301 YRATMSLQLLGQKRKTEGQLPFPLPPPPPMANISLNSSPSYRRTNSNRSAISSFGPSSPS 360
           Y+ATM LQLLGQKR T+G LPFPLP PPP+  IS N  P++RRT S+ S+ S +G SSPS
Sbjct: 301 YKATMRLQLLGQKRNTQGHLPFPLPRPPPITYISRNHFPNFRRTESDISSTSFYGSSSPS 360

Query: 361 HSVFSQSS--LSSSSSFNRICPKCYLNEKDLAFGCGHQTCSDCGQDIIFCPTCQTQITIR 420
           HS   +SS   S SSSFNRIC +CY+NEKDLA GCGHQ CS CG+D+I CP CQT ITIR
Sbjct: 361 HSALLRSSSVSSPSSSFNRICSQCYINEKDLALGCGHQICSACGEDLIACPICQTHITIR 420

Query: 421 IRLR 423
           IRLR
Sbjct: 421 IRLR 424

BLAST of Spg012047 vs. ExPASy TrEMBL
Match: A0A6J1GF30 (E3 ubiquitin-protein ligase RGLG5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453613 PE=4 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 3.5e-191
Identity = 330/424 (77.83%), Postives = 366/424 (86.32%), Query Frame = 0

Query: 1   MGGKPSKHIHCHLHIHGANQEQSSKCLCGRNSPPSTSFAPSFRSPSHKINGDDFESLEQV 60
           MG K SK+IHCH+HI+G ++ Q  +C CG + PPST+ A S R P+  INGDDFESLEQV
Sbjct: 1   MGAKSSKNIHCHVHIYGDHRNQPLRCSCGYSPPPSTNSARSSRPPNPIINGDDFESLEQV 60

Query: 61  TRALEQAGLESSNLIVGIDFTKSNEWTGKESFMGKNLHDLDGACLNPYEQAISIIGKTLQ 120
           + ALE+AGLESSNLIVGIDFT+SNEWTGK+SFMG+NLHDLDGACLNPYEQAISIIGKTL+
Sbjct: 61  SEALEKAGLESSNLIVGIDFTRSNEWTGKKSFMGRNLHDLDGACLNPYEQAISIIGKTLE 120

Query: 121 KFDDDNIIPCYGFGDATTGDQEVFSFHPEDRPCNGFEEVLFFYRERVPHVKLAGPTSFAP 180
           KFDDDNIIPCYGFGD TTGD++VFSFHP D PC+GFEEVL  YRE VPHV LAGPTSFAP
Sbjct: 121 KFDDDNIIPCYGFGDVTTGDRDVFSFHPGDTPCHGFEEVLHCYRETVPHVNLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSSGQYHILLIIADGQVTRSIDTPPGHLSPQEQSTVDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTRS+DT  GHLSPQEQST++AIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSYGQYHILLIIADGQVTRSVDTCQGHLSPQEQSTIEAIVEASKYPLSII 240

Query: 241 LVGVGDGPWDQMYECDDQIPARDFDNFQFVNFTDIMSKGISPSKKETEFALQCLMEIPPQ 300
           LVGVGDGPWDQMY+CDDQIPARDFDNFQFVNFTDIMSKG  PSKK+TEFALQCLMEIPPQ
Sbjct: 241 LVGVGDGPWDQMYQCDDQIPARDFDNFQFVNFTDIMSKGTPPSKKKTEFALQCLMEIPPQ 300

Query: 301 YRATMSLQLLGQKRKTEGQLPFPLPPPPPMANISLNSSPSYRRTNSNRSAISSFGPSSPS 360
           Y+ATM LQLLGQKR T+G LPFPLP PPP+  IS N  P++RRT S+ S+ S +G SSPS
Sbjct: 301 YKATMRLQLLGQKRNTQGHLPFPLPRPPPITYISRNHFPNFRRTESDISSTSFYGSSSPS 360

Query: 361 HSVFSQSS--LSSSSSFNRICPKCYLNEKDLAFGCGHQTCSDCGQDIIFCPTCQTQITIR 420
           HS   +SS   S S SFNRIC +CY+NEKDLA GCGHQ CS CG+D+I CP CQT IT R
Sbjct: 361 HSALLRSSSVSSPSLSFNRICSQCYINEKDLALGCGHQICSVCGEDLILCPICQTHITTR 420

Query: 421 IRLR 423
           IRLR
Sbjct: 421 IRLR 424

BLAST of Spg012047 vs. ExPASy TrEMBL
Match: A0A6J1GG07 (E3 ubiquitin-protein ligase RGLG5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453613 PE=4 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 4.2e-189
Identity = 331/435 (76.09%), Postives = 366/435 (84.14%), Query Frame = 0

Query: 1   MGGKPSKHIHCHLHIHGANQEQSSKCLCGRNSPPSTSFAPSFRSPSHK-----------I 60
           MG K SK+IHCH+HI+G ++ Q  +C CG + PP+TS  PS  SPS             I
Sbjct: 1   MGAKSSKNIHCHVHIYGDHRNQPLRCSCGYSPPPTTSPWPSSPSPSTNSARSSRPPNPII 60

Query: 61  NGDDFESLEQVTRALEQAGLESSNLIVGIDFTKSNEWTGKESFMGKNLHDLDGACLNPYE 120
           NGDDFESLEQV+ ALE+AGLESSNLIVGIDFT+SNEWTGK+SFMG+NLHDLDGACLNPYE
Sbjct: 61  NGDDFESLEQVSEALEKAGLESSNLIVGIDFTRSNEWTGKKSFMGRNLHDLDGACLNPYE 120

Query: 121 QAISIIGKTLQKFDDDNIIPCYGFGDATTGDQEVFSFHPEDRPCNGFEEVLFFYRERVPH 180
           QAISIIGKTL+KFDDDNIIPCYGFGD TTGD++VFSFHP D PC+GFEEVL  YRE VPH
Sbjct: 121 QAISIIGKTLEKFDDDNIIPCYGFGDVTTGDRDVFSFHPGDTPCHGFEEVLHCYRETVPH 180

Query: 181 VKLAGPTSFAPIIRNAIRIVNDSSGQYHILLIIADGQVTRSIDTPPGHLSPQEQSTVDAI 240
           V LAGPTSFAPIIRNAIRIVNDS GQYHILLIIADGQVTRS+DT  GHLSPQEQST++AI
Sbjct: 181 VNLAGPTSFAPIIRNAIRIVNDSYGQYHILLIIADGQVTRSVDTCQGHLSPQEQSTIEAI 240

Query: 241 VEASKYPLSIILVGVGDGPWDQMYECDDQIPARDFDNFQFVNFTDIMSKGISPSKKETEF 300
           VEASKYPLSIILVGVGDGPWDQMY+CDDQIPARDFDNFQFVNFTDIMSKG  PSKK+TEF
Sbjct: 241 VEASKYPLSIILVGVGDGPWDQMYQCDDQIPARDFDNFQFVNFTDIMSKGTPPSKKKTEF 300

Query: 301 ALQCLMEIPPQYRATMSLQLLGQKRKTEGQLPFPLPPPPPMANISLNSSPSYRRTNSNRS 360
           ALQCLMEIPPQY+ATM LQLLGQKR T+G LPFPLP PPP+  IS N  P++RRT S+ S
Sbjct: 301 ALQCLMEIPPQYKATMRLQLLGQKRNTQGHLPFPLPRPPPITYISRNHFPNFRRTESDIS 360

Query: 361 AISSFGPSSPSHSVFSQSS--LSSSSSFNRICPKCYLNEKDLAFGCGHQTCSDCGQDIIF 420
           + S +G SSPSHS   +SS   S S SFNRIC +CY+NEKDLA GCGHQ CS CG+D+I 
Sbjct: 361 STSFYGSSSPSHSALLRSSSVSSPSLSFNRICSQCYINEKDLALGCGHQICSVCGEDLIL 420

Query: 421 CPTCQTQITIRIRLR 423
           CP CQT IT RIRLR
Sbjct: 421 CPICQTHITTRIRLR 435

BLAST of Spg012047 vs. TAIR 10
Match: AT5G14420.1 (RING domain ligase2 )

HSP 1 Score: 449.5 bits (1155), Expect = 3.0e-126
Identity = 241/401 (60.10%), Postives = 284/401 (70.82%), Query Frame = 0

Query: 31  NSPPSTSFAPSFR---SPSHKINGDDFESLEQVTRALEQAGLESSNLIVGIDFTKSNEWT 90
           ++PPS S+    +      +    DD+ SLEQVT AL +AGLESSNLIVGIDFTKSNEWT
Sbjct: 77  SAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLIVGIDFTKSNEWT 136

Query: 91  GKESFMGKNLHDLDGACLNPYEQAISIIGKTLQKFDDDNIIPCYGFGDATTGDQEVFSFH 150
           G  SF  K+LH + G+  NPYEQAI+IIG+TL  FD+DN+IPCYGFGDA+T DQ+VFSF+
Sbjct: 137 GARSFNRKSLHFI-GSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFN 196

Query: 151 PEDRPCNGFEEVLFFYRERVPHVKLAGPTSFAPIIRNAIRIVNDSSGQYHILLIIADGQV 210
            EDR CNGFEEVL  Y+E VP +KLAGPTSFAPII  A+ IV  S GQYH+L+IIADGQV
Sbjct: 197 SEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVIIADGQV 256

Query: 211 TRSIDTPPGHLSPQEQSTVDAIVEASKYPLSIILVGVGDGPWDQMYECDDQIPARDFDNF 270
           TRS+DT  G LSPQEQ TVDAIV+ASK PLSI+LVGVGDGPWD M E DD IPAR FDNF
Sbjct: 257 TRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPARAFDNF 316

Query: 271 QFVNFTDIMSKGISPSKKETEFALQCLMEIPPQYRATMSLQLLGQKRKTEGQLPFPLPPP 330
           QFVNFT+IM+K  + S KETEFAL  LMEIP QY+AT+ L LLG++    G +P   P P
Sbjct: 317 QFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGRR---NGYIPERFPLP 376

Query: 331 PPMANISLNSSPSYRRTNSNRSAISSFGPSSPSHSVFSQSSLS-------SSSSFNRICP 390
           PPM       S SY     +R  + SF PS P H        S       SS+S N++CP
Sbjct: 377 PPMR----GGSSSYNSPKPSR--LPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCP 436

Query: 391 KCYLNEKDLAFGCGHQTCSDCGQDIIFCPTCQTQITIRIRL 422
            C  N KD+AFGCGHQTC +CG D+  CP C+  I  RI+L
Sbjct: 437 ICLSNPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKL 467

BLAST of Spg012047 vs. TAIR 10
Match: AT5G14420.3 (RING domain ligase2 )

HSP 1 Score: 449.5 bits (1155), Expect = 3.0e-126
Identity = 241/401 (60.10%), Postives = 284/401 (70.82%), Query Frame = 0

Query: 31  NSPPSTSFAPSFR---SPSHKINGDDFESLEQVTRALEQAGLESSNLIVGIDFTKSNEWT 90
           ++PPS S+    +      +    DD+ SLEQVT AL +AGLESSNLIVGIDFTKSNEWT
Sbjct: 77  SAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLIVGIDFTKSNEWT 136

Query: 91  GKESFMGKNLHDLDGACLNPYEQAISIIGKTLQKFDDDNIIPCYGFGDATTGDQEVFSFH 150
           G  SF  K+LH + G+  NPYEQAI+IIG+TL  FD+DN+IPCYGFGDA+T DQ+VFSF+
Sbjct: 137 GARSFNRKSLHFI-GSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFN 196

Query: 151 PEDRPCNGFEEVLFFYRERVPHVKLAGPTSFAPIIRNAIRIVNDSSGQYHILLIIADGQV 210
            EDR CNGFEEVL  Y+E VP +KLAGPTSFAPII  A+ IV  S GQYH+L+IIADGQV
Sbjct: 197 SEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVIIADGQV 256

Query: 211 TRSIDTPPGHLSPQEQSTVDAIVEASKYPLSIILVGVGDGPWDQMYECDDQIPARDFDNF 270
           TRS+DT  G LSPQEQ TVDAIV+ASK PLSI+LVGVGDGPWD M E DD IPAR FDNF
Sbjct: 257 TRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPARAFDNF 316

Query: 271 QFVNFTDIMSKGISPSKKETEFALQCLMEIPPQYRATMSLQLLGQKRKTEGQLPFPLPPP 330
           QFVNFT+IM+K  + S KETEFAL  LMEIP QY+AT+ L LLG++    G +P   P P
Sbjct: 317 QFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGRR---NGYIPERFPLP 376

Query: 331 PPMANISLNSSPSYRRTNSNRSAISSFGPSSPSHSVFSQSSLS-------SSSSFNRICP 390
           PPM       S SY     +R  + SF PS P H        S       SS+S N++CP
Sbjct: 377 PPMR----GGSSSYNSPKPSR--LPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCP 436

Query: 391 KCYLNEKDLAFGCGHQTCSDCGQDIIFCPTCQTQITIRIRL 422
            C  N KD+AFGCGHQTC +CG D+  CP C+  I  RI+L
Sbjct: 437 ICLSNPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKL 467

BLAST of Spg012047 vs. TAIR 10
Match: AT5G14420.4 (RING domain ligase2 )

HSP 1 Score: 449.5 bits (1155), Expect = 3.0e-126
Identity = 241/401 (60.10%), Postives = 284/401 (70.82%), Query Frame = 0

Query: 31  NSPPSTSFAPSFR---SPSHKINGDDFESLEQVTRALEQAGLESSNLIVGIDFTKSNEWT 90
           ++PPS S+    +      +    DD+ SLEQVT AL +AGLESSNLIVGIDFTKSNEWT
Sbjct: 77  SAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLIVGIDFTKSNEWT 136

Query: 91  GKESFMGKNLHDLDGACLNPYEQAISIIGKTLQKFDDDNIIPCYGFGDATTGDQEVFSFH 150
           G  SF  K+LH + G+  NPYEQAI+IIG+TL  FD+DN+IPCYGFGDA+T DQ+VFSF+
Sbjct: 137 GARSFNRKSLHFI-GSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFN 196

Query: 151 PEDRPCNGFEEVLFFYRERVPHVKLAGPTSFAPIIRNAIRIVNDSSGQYHILLIIADGQV 210
            EDR CNGFEEVL  Y+E VP +KLAGPTSFAPII  A+ IV  S GQYH+L+IIADGQV
Sbjct: 197 SEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVIIADGQV 256

Query: 211 TRSIDTPPGHLSPQEQSTVDAIVEASKYPLSIILVGVGDGPWDQMYECDDQIPARDFDNF 270
           TRS+DT  G LSPQEQ TVDAIV+ASK PLSI+LVGVGDGPWD M E DD IPAR FDNF
Sbjct: 257 TRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPARAFDNF 316

Query: 271 QFVNFTDIMSKGISPSKKETEFALQCLMEIPPQYRATMSLQLLGQKRKTEGQLPFPLPPP 330
           QFVNFT+IM+K  + S KETEFAL  LMEIP QY+AT+ L LLG++    G +P   P P
Sbjct: 317 QFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGRR---NGYIPERFPLP 376

Query: 331 PPMANISLNSSPSYRRTNSNRSAISSFGPSSPSHSVFSQSSLS-------SSSSFNRICP 390
           PPM       S SY     +R  + SF PS P H        S       SS+S N++CP
Sbjct: 377 PPMR----GGSSSYNSPKPSR--LPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCP 436

Query: 391 KCYLNEKDLAFGCGHQTCSDCGQDIIFCPTCQTQITIRIRL 422
            C  N KD+AFGCGHQTC +CG D+  CP C+  I  RI+L
Sbjct: 437 ICLSNPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKL 467

BLAST of Spg012047 vs. TAIR 10
Match: AT5G14420.2 (RING domain ligase2 )

HSP 1 Score: 449.5 bits (1155), Expect = 3.0e-126
Identity = 241/401 (60.10%), Postives = 284/401 (70.82%), Query Frame = 0

Query: 31  NSPPSTSFAPSFR---SPSHKINGDDFESLEQVTRALEQAGLESSNLIVGIDFTKSNEWT 90
           ++PPS S+    +      +    DD+ SLEQVT AL +AGLESSNLIVGIDFTKSNEWT
Sbjct: 77  SAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLIVGIDFTKSNEWT 136

Query: 91  GKESFMGKNLHDLDGACLNPYEQAISIIGKTLQKFDDDNIIPCYGFGDATTGDQEVFSFH 150
           G  SF  K+LH + G+  NPYEQAI+IIG+TL  FD+DN+IPCYGFGDA+T DQ+VFSF+
Sbjct: 137 GARSFNRKSLHFI-GSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFN 196

Query: 151 PEDRPCNGFEEVLFFYRERVPHVKLAGPTSFAPIIRNAIRIVNDSSGQYHILLIIADGQV 210
            EDR CNGFEEVL  Y+E VP +KLAGPTSFAPII  A+ IV  S GQYH+L+IIADGQV
Sbjct: 197 SEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVIIADGQV 256

Query: 211 TRSIDTPPGHLSPQEQSTVDAIVEASKYPLSIILVGVGDGPWDQMYECDDQIPARDFDNF 270
           TRS+DT  G LSPQEQ TVDAIV+ASK PLSI+LVGVGDGPWD M E DD IPAR FDNF
Sbjct: 257 TRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPARAFDNF 316

Query: 271 QFVNFTDIMSKGISPSKKETEFALQCLMEIPPQYRATMSLQLLGQKRKTEGQLPFPLPPP 330
           QFVNFT+IM+K  + S KETEFAL  LMEIP QY+AT+ L LLG++    G +P   P P
Sbjct: 317 QFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGRR---NGYIPERFPLP 376

Query: 331 PPMANISLNSSPSYRRTNSNRSAISSFGPSSPSHSVFSQSSLS-------SSSSFNRICP 390
           PPM       S SY     +R  + SF PS P H        S       SS+S N++CP
Sbjct: 377 PPMR----GGSSSYNSPKPSR--LPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCP 436

Query: 391 KCYLNEKDLAFGCGHQTCSDCGQDIIFCPTCQTQITIRIRL 422
            C  N KD+AFGCGHQTC +CG D+  CP C+  I  RI+L
Sbjct: 437 ICLSNPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKL 467

BLAST of Spg012047 vs. TAIR 10
Match: AT3G01650.1 (RING domain ligase1 )

HSP 1 Score: 441.0 bits (1133), Expect = 1.0e-123
Identity = 241/411 (58.64%), Postives = 287/411 (69.83%), Query Frame = 0

Query: 16  HGANQEQSSKCLCGRNSPPSTSFA-----PSFRSPSHKINGDDFESLEQVTRALEQAGLE 75
           H  +QE  +      N PP  S +       F     KI+ D++ SL QV+ AL +AGLE
Sbjct: 95  HQYSQEGYASAASQPNYPPPPSQSQVADRKKFDRRYSKIS-DNYSSLLQVSEALGRAGLE 154

Query: 76  SSNLIVGIDFTKSNEWTGKESFMGKNLHDLDGACLNPYEQAISIIGKTLQKFDDDNIIPC 135
           SSNLIVGIDFTKSNEWTG +SF  K+LH L     NPYEQAI+IIG+TL  FD+DN+IPC
Sbjct: 155 SSNLIVGIDFTKSNEWTGAKSFNRKSLHHLSNT-PNPYEQAITIIGRTLAAFDEDNLIPC 214

Query: 136 YGFGDATTGDQEVFSFHPEDRPCNGFEEVLFFYRERVPHVKLAGPTSFAPIIRNAIRIVN 195
           YGFGDA+T DQ+VFSF+PE R CNGFEEVL  YRE VP +KLAGPTSFAPII  A+ +V 
Sbjct: 215 YGFGDASTHDQDVFSFYPEGRFCNGFEEVLARYREIVPQLKLAGPTSFAPIIEMAMTVVE 274

Query: 196 DSSGQYHILLIIADGQVTRSIDTPPGHLSPQEQSTVDAIVEASKYPLSIILVGVGDGPWD 255
            SSGQYH+L+IIADGQVTRS+DT  G LSPQEQ TVDAIV+AS  PLSI+LVGVGDGPWD
Sbjct: 275 QSSGQYHVLVIIADGQVTRSVDTEHGRLSPQEQKTVDAIVKASTLPLSIVLVGVGDGPWD 334

Query: 256 QMYECDDQIPARDFDNFQFVNFTDIMSKGISPSKKETEFALQCLMEIPPQYRATMSLQLL 315
            M E DD IPAR FDNFQFVNFT+IMSK    S+KETEFAL  LMEIPPQY+AT+ L LL
Sbjct: 335 MMQEFDDNIPARAFDNFQFVNFTEIMSKNKDQSRKETEFALSALMEIPPQYKATIELNLL 394

Query: 316 GQKRKTEGQLPFPLPPPPPMANISLNSSPSYRRTNSNRSAISSFGPSSPSHSVFSQSSLS 375
           G +    G +P  +P PPP+ + S  SS          S I +F PS P +  F    +S
Sbjct: 395 GVR---NGNIPQRIPLPPPVQSGSSFSS----------SRIPNFEPSVPPYP-FESKQMS 454

Query: 376 SSSSFNRICPKCYLNEKDLAFGCGHQTCSDCGQDIIFCPTCQTQITIRIRL 422
           S+    ++CP C  N K++AFGCGHQTC +CG D+  CP C+  I  RI+L
Sbjct: 455 SADDI-QLCPICLSNPKNMAFGCGHQTCCECGPDLKVCPICRAPIQTRIKL 488