Spg011711 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg011711
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionmitogen-activated protein kinase kinase kinase 18-like
Locationscaffold1: 4000937 .. 4002376 (-)
RNA-Seq ExpressionSpg011711
SyntenySpg011711
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTGGACGAGAGGCCGGATCATTGGCTCTGGCTCCTCCGCCGCGGTCTCCGTCGCCACTGATCTCCGGTCCGGTCAACTGCTGGCGGTTAAATCTGTTGATTTCGTCCACTCGGATTTTCTTATGAGGGAACAGAGGATTCTTTCTCAATTGAATTGTCCAAATGTTGTAGCTTATAAGGGTTTTGATGTTACGTTGGAAAATGGGAATTTGATGTGCAATCTTTTGATGGAGTTTGCGCCTGGAGGGTCGATTTTGGATGCGATGGAGGGGGCTGGTGGCCGATTAGATGAGGCGACGGCCCAATTGTATACTCGAGAGATTTTAAATGGGCTCGAGTATGTTCATTCTAATGGTGTGGTGCATTGTGACATTAAGTGTTGTAATATTTTGGTCGGTGAAGATGGGATTAAGATTGCGGATTTCGGCTGCGCGCGGCGGGTAGAGGACGTTTCCGGTGGAAATTTTGCCGGAACGCCGATATTCATGGCGCCGGAGGTGGCGCGTGGAGAAAAACAGGGGTTTGCGGCTGATGTTTGGGCGGTGGGTTGCACGGTGATCCAGATGGTGACCGGCGGAGCCCCGTGGGCGGATGTGTCGGATCCGGTGGCCGCCATTTACAGAATTGGGTCCGGCGATGACTTGCCGGAGATTCCGAGGTTCATGTCGGAGCAGGGCAAGGACTTTCTGCGGCGGTGTTTGATCCGAGACCCAGAAGAACGGTGGTCTGTTAGTGAGCTTTTGAAACACCCTTTTGTTCAGGAGCAGAAATTTCATCCCAAACAGAATTCAAGAACGCCGACGAGTATTTTGGAGCAAGGATTATGGGACACGATGAACGATTTGGAAACAGTTCAAAGTCCGATCAGACCAAAAATTTACAGAACGCCATTGCAGAGGATTCAGCAACTGAGCGAAGTGTCCAAATTTCAGTCTTTTGGAATGCCCAATTGGGAGTGCGACGAAGATTGGGTAACAGTGAGAAGCAGAGGCATGGGAGAGGAAGACAGAGAAAAAAACGATTTCGTCTCTGTATTGGAATTGCCCTGTTCCTCTGAATCCATGGCAATGACAACTAAAAATGAAGTTGACGATCAGAATTATGTTAGTATTAATATCAATAGAACCTATGATTGTTGTAGCAAAAAATGCAGCAAGGCTAAGAAGAGGGACTTGCTAATGTCCTCTGTTTCTAATCAGATTGTAACATTCCAAACTATTAATCTTTCTAATAATGACAGTTTATGTATTATCATTTCTTTGTTTCTTGTTTTTCCATCCAGCCGTTCAGTTTATCTTATATTCTTAAGATATAATGACAACGTTTTGATTTATCGAGATATCGACGAAATATCAACATGTCGACGTGTTCACCATGTACTTGAGTTATCTTGTTTGGAGACGGAAGCTTGCCTTGGAAAATTTACTAAGATGTGA

mRNA sequence

ATGGAGTGGACGAGAGGCCGGATCATTGGCTCTGGCTCCTCCGCCGCGGTCTCCGTCGCCACTGATCTCCGGTCCGGTCAACTGCTGGCGGTTAAATCTGTTGATTTCGTCCACTCGGATTTTCTTATGAGGGAACAGAGGATTCTTTCTCAATTGAATTGTCCAAATGTTGTAGCTTATAAGGGTTTTGATGTTACGTTGGAAAATGGGAATTTGATGTGCAATCTTTTGATGGAGTTTGCGCCTGGAGGGTCGATTTTGGATGCGATGGAGGGGGCTGGTGGCCGATTAGATGAGGCGACGGCCCAATTGTATACTCGAGAGATTTTAAATGGGCTCGAGTATGTTCATTCTAATGGTGTGGTGCATTGTGACATTAAGTGTTGTAATATTTTGGTCGGTGAAGATGGGATTAAGATTGCGGATTTCGGCTGCGCGCGGCGGGTAGAGGACGTTTCCGGTGGAAATTTTGCCGGAACGCCGATATTCATGGCGCCGGAGGTGGCGCGTGGAGAAAAACAGGGGTTTGCGGCTGATGTTTGGGCGGTGGGTTGCACGGTGATCCAGATGGTGACCGGCGGAGCCCCGTGGGCGGATGTGTCGGATCCGGTGGCCGCCATTTACAGAATTGGGTCCGGCGATGACTTGCCGGAGATTCCGAGGTTCATGTCGGAGCAGGGCAAGGACTTTCTGCGGCGGTGTTTGATCCGAGACCCAGAAGAACGGTGGTCTGTTAGTGAGCTTTTGAAACACCCTTTTGTTCAGGAGCAGAAATTTCATCCCAAACAGAATTCAAGAACGCCGACGAGTATTTTGGAGCAAGGATTATGGGACACGATGAACGATTTGGAAACAGTTCAAAGTCCGATCAGACCAAAAATTTACAGAACGCCATTGCAGAGGATTCAGCAACTGAGCGAAGTGTCCAAATTTCAGTCTTTTGGAATGCCCAATTGGGAGTGCGACGAAGATTGGGTAACAGTGAGAAGCAGAGGCATGGGAGAGGAAGACAGAGAAAAAAACGATTTCGTCTCTGTATTGGAATTGCCCTGTTCCTCTGAATCCATGGCAATGACAACTAAAAATGAAGTTGACGATCAGAATTATGTTAGTATTAATATCAATAGAACCTATGATTGTTGTAGCAAAAAATGCAGCAAGGCTAAGAAGAGGGACTTGCTAATGTCCTCTGTTTCTAATCAGATTGTAACATTCCAAACTATTAATCTTTCTAATAATGACAGTTTATGTATTATCATTTCTTTGTTTCTTGTTTTTCCATCCAGCCGTTCAGTTTATCTTATATTCTTAAGATATAATGACAACGTTTTGATTTATCGAGATATCGACGAAATATCAACATGTCGACGTGTTCACCATGTACTTGAGTTATCTTGTTTGGAGACGGAAGCTTGCCTTGGAAAATTTACTAAGATGTGA

Coding sequence (CDS)

ATGGAGTGGACGAGAGGCCGGATCATTGGCTCTGGCTCCTCCGCCGCGGTCTCCGTCGCCACTGATCTCCGGTCCGGTCAACTGCTGGCGGTTAAATCTGTTGATTTCGTCCACTCGGATTTTCTTATGAGGGAACAGAGGATTCTTTCTCAATTGAATTGTCCAAATGTTGTAGCTTATAAGGGTTTTGATGTTACGTTGGAAAATGGGAATTTGATGTGCAATCTTTTGATGGAGTTTGCGCCTGGAGGGTCGATTTTGGATGCGATGGAGGGGGCTGGTGGCCGATTAGATGAGGCGACGGCCCAATTGTATACTCGAGAGATTTTAAATGGGCTCGAGTATGTTCATTCTAATGGTGTGGTGCATTGTGACATTAAGTGTTGTAATATTTTGGTCGGTGAAGATGGGATTAAGATTGCGGATTTCGGCTGCGCGCGGCGGGTAGAGGACGTTTCCGGTGGAAATTTTGCCGGAACGCCGATATTCATGGCGCCGGAGGTGGCGCGTGGAGAAAAACAGGGGTTTGCGGCTGATGTTTGGGCGGTGGGTTGCACGGTGATCCAGATGGTGACCGGCGGAGCCCCGTGGGCGGATGTGTCGGATCCGGTGGCCGCCATTTACAGAATTGGGTCCGGCGATGACTTGCCGGAGATTCCGAGGTTCATGTCGGAGCAGGGCAAGGACTTTCTGCGGCGGTGTTTGATCCGAGACCCAGAAGAACGGTGGTCTGTTAGTGAGCTTTTGAAACACCCTTTTGTTCAGGAGCAGAAATTTCATCCCAAACAGAATTCAAGAACGCCGACGAGTATTTTGGAGCAAGGATTATGGGACACGATGAACGATTTGGAAACAGTTCAAAGTCCGATCAGACCAAAAATTTACAGAACGCCATTGCAGAGGATTCAGCAACTGAGCGAAGTGTCCAAATTTCAGTCTTTTGGAATGCCCAATTGGGAGTGCGACGAAGATTGGGTAACAGTGAGAAGCAGAGGCATGGGAGAGGAAGACAGAGAAAAAAACGATTTCGTCTCTGTATTGGAATTGCCCTGTTCCTCTGAATCCATGGCAATGACAACTAAAAATGAAGTTGACGATCAGAATTATGTTAGTATTAATATCAATAGAACCTATGATTGTTGTAGCAAAAAATGCAGCAAGGCTAAGAAGAGGGACTTGCTAATGTCCTCTGTTTCTAATCAGATTGTAACATTCCAAACTATTAATCTTTCTAATAATGACAGTTTATGTATTATCATTTCTTTGTTTCTTGTTTTTCCATCCAGCCGTTCAGTTTATCTTATATTCTTAAGATATAATGACAACGTTTTGATTTATCGAGATATCGACGAAATATCAACATGTCGACGTGTTCACCATGTACTTGAGTTATCTTGTTTGGAGACGGAAGCTTGCCTTGGAAAATTTACTAAGATGTGA

Protein sequence

MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDFVHSDFLMREQRILSQLNCPNVVAYKGFDVTLENGNLMCNLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREILNGLEYVHSNGVVHCDIKCCNILVGEDGIKIADFGCARRVEDVSGGNFAGTPIFMAPEVARGEKQGFAADVWAVGCTVIQMVTGGAPWADVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRDPEERWSVSELLKHPFVQEQKFHPKQNSRTPTSILEQGLWDTMNDLETVQSPIRPKIYRTPLQRIQQLSEVSKFQSFGMPNWECDEDWVTVRSRGMGEEDREKNDFVSVLELPCSSESMAMTTKNEVDDQNYVSININRTYDCCSKKCSKAKKRDLLMSSVSNQIVTFQTINLSNNDSLCIIISLFLVFPSSRSVYLIFLRYNDNVLIYRDIDEISTCRRVHHVLELSCLETEACLGKFTKM
Homology
BLAST of Spg011711 vs. NCBI nr
Match: XP_022926885.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita moschata])

HSP 1 Score: 709.9 bits (1831), Expect = 1.5e-200
Identity = 362/453 (79.91%), Postives = 390/453 (86.09%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDFVHSDFLMREQRILSQLNCPNVVAY 60
           MEWTRGR+IG GSSAAVSVATDLRSGQLLAVKSV+F  SDF+ REQRILSQLN   V+A 
Sbjct: 13  MEWTRGRVIGCGSSAAVSVATDLRSGQLLAVKSVEFDRSDFIKREQRILSQLNSSRVIAC 72

Query: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREILNGLEYVHSNG 120
           KGFDVTLENGNLMCNLLMEFA GGSILDAME AGGRLDEATAQ YTREIL+GLEYVHSNG
Sbjct: 73  KGFDVTLENGNLMCNLLMEFASGGSILDAMEAAGGRLDEATAQFYTREILSGLEYVHSNG 132

Query: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVSGGNFAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKC NILVGEDGIKIADFGCARRVE+VSGGN AGTPIFMAPEVARGEKQGFAADV
Sbjct: 133 VVHCDIKCSNILVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 192

Query: 181 WAVGCTVIQMVTGGAPWADVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRDPE 240
           WAVGCTVIQMVTG APW D+SDPVAAIYRIGS DDLPEIPRFMSEQGK+FLRRCLIRDPE
Sbjct: 193 WAVGCTVIQMVTGRAPWEDMSDPVAAIYRIGSSDDLPEIPRFMSEQGKEFLRRCLIRDPE 252

Query: 241 ERWSVSELLKHPFVQEQKFHPKQNSRTPTSILEQGLWDTMNDLETVQSPIRPKIYRTPLQ 300
           ERWSVSELLKHPFV EQK  PKQN RTPTSILEQ LW+TMNDLETVQSPIRPK YRTPLQ
Sbjct: 253 ERWSVSELLKHPFVHEQKSRPKQNPRTPTSILEQELWNTMNDLETVQSPIRPKFYRTPLQ 312

Query: 301 RIQQLSEVSKFQSFGMPNWECDEDWVTVRSRGM--GEEDREKNDFVSVLELPCSSESMAM 360
           RIQQLSEVSKFQSFG+PNWE +EDWVTVRS+ M   EEDREK DF+S LELPCSSESMA 
Sbjct: 313 RIQQLSEVSKFQSFGIPNWESNEDWVTVRSKDMEEEEEDREKRDFISELELPCSSESMAT 372

Query: 361 TTKNEVDDQNYVSININRTYDCCSKKCSKAKKRDLLMSSVSNQIVTFQTINLSNNDSLCI 420
           TT+N   DQNY+SI+ +  Y CC       KK +  MSSV +QIV+   +N  NN+ LC 
Sbjct: 373 TTQNGAGDQNYISISKSSDY-CC-------KKINFPMSSVFSQIVSLNIVNFCNNNKLCY 432

Query: 421 IISLFLVFPSSRSVYLIFLRYNDNVLIYRDIDE 452
           +ISL LVF S+ SV+L   RYN+ +LIY +IDE
Sbjct: 433 VISLLLVFLSASSVHLTSFRYNEFILIYHNIDE 457

BLAST of Spg011711 vs. NCBI nr
Match: XP_023518154.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 708.4 bits (1827), Expect = 4.3e-200
Identity = 362/453 (79.91%), Postives = 388/453 (85.65%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDFVHSDFLMREQRILSQLNCPNVVAY 60
           MEWTRGR+IG GSSAAVSVATDLRSGQLLAVKSV+F  SDFL REQRILSQLN   V+A 
Sbjct: 1   MEWTRGRVIGCGSSAAVSVATDLRSGQLLAVKSVEFDRSDFLKREQRILSQLNSSRVIAC 60

Query: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREILNGLEYVHSNG 120
           KGFDVTLENGNLMCNLLMEFA GGSILDAME AGGRLDEATAQ YTREIL+GLEYVHSNG
Sbjct: 61  KGFDVTLENGNLMCNLLMEFASGGSILDAMEAAGGRLDEATAQFYTREILSGLEYVHSNG 120

Query: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVSGGNFAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKC NILVGEDGIKIADFGCARRVE+VSGGN AGTPIFMAPEVARGEKQGFAADV
Sbjct: 121 VVHCDIKCSNILVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 180

Query: 181 WAVGCTVIQMVTGGAPWADVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRDPE 240
           WAVGCTVIQMVTG APW DVSDPVAAIYRIGS DDLPEIPRFMSEQGK+FLRRCLIRDPE
Sbjct: 181 WAVGCTVIQMVTGRAPWEDVSDPVAAIYRIGSSDDLPEIPRFMSEQGKEFLRRCLIRDPE 240

Query: 241 ERWSVSELLKHPFVQEQKFHPKQNSRTPTSILEQGLWDTMNDLETVQSPIRPKIYRTPLQ 300
           ERWSVSELLKHPFV EQK  PKQN RTPTSILEQGLW+ MNDLETVQSPIRPK YRTPLQ
Sbjct: 241 ERWSVSELLKHPFVHEQKSRPKQNPRTPTSILEQGLWNMMNDLETVQSPIRPKFYRTPLQ 300

Query: 301 RIQQLSEVSKFQSFGMPNWECDEDWVTVRSRGM--GEEDREKNDFVSVLELPCSSESMAM 360
           RIQQLSEVSKFQSFG+PNWE +EDWVTVRS+ M   EEDREK DF+S LELPCSSE+MA 
Sbjct: 301 RIQQLSEVSKFQSFGIPNWESNEDWVTVRSKDMEEEEEDREKRDFISELELPCSSEAMAT 360

Query: 361 TTKNEVDDQNYVSININRTYDCCSKKCSKAKKRDLLMSSVSNQIVTFQTINLSNNDSLCI 420
           TT+N   DQNY  I+ +  Y CC       KK +  MSSV +QIV+   IN  NN+ LC 
Sbjct: 361 TTQNGAGDQNYTRISKSSDY-CC-------KKINFPMSSVFSQIVSLNIINFCNNNKLCY 420

Query: 421 IISLFLVFPSSRSVYLIFLRYNDNVLIYRDIDE 452
           +ISL LVF S+ SV+L   RYN+ +L+Y +IDE
Sbjct: 421 VISLLLVFLSASSVHLTSFRYNEFILVYHNIDE 445

BLAST of Spg011711 vs. NCBI nr
Match: XP_023003448.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita maxima])

HSP 1 Score: 707.2 bits (1824), Expect = 9.5e-200
Identity = 362/455 (79.56%), Postives = 390/455 (85.71%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDFVHSDFLMREQRILSQLNCPNVVAY 60
           MEWTRGR+IG GSSAAVSVATDLRSGQLLAVKSV+F  SDFL REQRILSQLN   V+A 
Sbjct: 1   MEWTRGRVIGCGSSAAVSVATDLRSGQLLAVKSVEFDRSDFLKREQRILSQLNSSRVIAC 60

Query: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREILNGLEYVHSNG 120
           KGFDVTLENGNLMCNLLMEFA GGSILDAME AGGRLDEATAQ YTRE+L+GLEYVHSNG
Sbjct: 61  KGFDVTLENGNLMCNLLMEFASGGSILDAMEAAGGRLDEATAQFYTREVLSGLEYVHSNG 120

Query: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVSGGNFAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKC NILVGEDGIKIADFGCARRVE+VS GN AGTPIFMAPEVARGEKQGFAADV
Sbjct: 121 VVHCDIKCSNILVGEDGIKIADFGCARRVEEVSCGNLAGTPIFMAPEVARGEKQGFAADV 180

Query: 181 WAVGCTVIQMVTGGAPWADVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRDPE 240
           WAVGCTVIQMVTG APW D+SDPVAAIYRIGS DDLPEIPRFMSEQGKDFLRRCLIRDPE
Sbjct: 181 WAVGCTVIQMVTGRAPWEDLSDPVAAIYRIGSSDDLPEIPRFMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVSELLKHPFVQEQKFHPKQNSRTPTSILEQGLWDTMNDLETVQSPIRPKIYRTPLQ 300
           ERWS+SELLKHPFV EQK  PKQN RTPTSILEQGLW+TMNDL TVQSPIRPK YRTPLQ
Sbjct: 241 ERWSLSELLKHPFVHEQKSRPKQNPRTPTSILEQGLWNTMNDLGTVQSPIRPKFYRTPLQ 300

Query: 301 RIQQLSEVSKFQSFGMPNWECDEDWVTVRSRGM----GEEDREKNDFVSVLELPCSSESM 360
           RIQQLSEVSKFQSFG+PNWE +EDWVTVRS+ M     EEDREK DF+S LELPCSSESM
Sbjct: 301 RIQQLSEVSKFQSFGIPNWESNEDWVTVRSKDMEEEEEEEDREKRDFISELELPCSSESM 360

Query: 361 AMTTKNEVDDQNYVSININRTYDCCSKKCSKAKKRDLLMSSVSNQIVTFQTINLSNNDSL 420
           A TT+N   DQNY+SI+ +  Y CC       KK +  MSSV +QIV+   IN  NN++L
Sbjct: 361 ATTTQNGAGDQNYISISESSDY-CC-------KKINFPMSSVFSQIVSLNFINFCNNNNL 420

Query: 421 CIIISLFLVFPSSRSVYLIFLRYNDNVLIYRDIDE 452
           C +ISL LVF S+ SV+L   RYN+ +LIY +IDE
Sbjct: 421 CYVISLLLVFLSASSVHLTSFRYNEFILIYHNIDE 447

BLAST of Spg011711 vs. NCBI nr
Match: XP_038883085.1 (mitogen-activated protein kinase kinase kinase 18-like [Benincasa hispida])

HSP 1 Score: 702.6 bits (1812), Expect = 2.4e-198
Identity = 360/486 (74.07%), Postives = 395/486 (81.28%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDFVHSDFLMREQRILSQLNCPNVVAY 60
           MEW RGR IG GSSAAVSVATDLRSGQ LAVKSV+F H DFL REQRILSQLNC NV+ Y
Sbjct: 1   MEWMRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHLDFLKREQRILSQLNCSNVIGY 60

Query: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREILNGLEYVHSNG 120
           KGFDVTLE+GNLMCNLLMEFA GGSILDAME AGGRLDEATAQLYTREIL+GLEYVHSNG
Sbjct: 61  KGFDVTLEDGNLMCNLLMEFASGGSILDAMEEAGGRLDEATAQLYTREILSGLEYVHSNG 120

Query: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVSGGNFAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCNILVGEDGIKIADFGCARRVE+VSGGN+AGTPIFMAPEVARG+KQGFAADV
Sbjct: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEEVSGGNWAGTPIFMAPEVARGKKQGFAADV 180

Query: 181 WAVGCTVIQMVTGGAPWADVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRDPE 240
           W VGC VIQMVTG  PWA+VSDPVAAIYRIG+GD+LPEIPRFMSEQGKDFLRRCLIRDPE
Sbjct: 181 WGVGCAVIQMVTGRPPWANVSDPVAAIYRIGAGDELPEIPRFMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVSELLKHPFVQEQKFHPKQNSRTP-------------------------------- 300
           +RWSV+ELLKHPFV EQK HPKQNSRTP                                
Sbjct: 241 KRWSVNELLKHPFVYEQKSHPKQNSRTPRSILEQGLWDTMNDSETIRSPVQSPIRRKIHR 300

Query: 301 -----TSILEQGLWDTMNDLETVQSPIRPKIYRTPLQRIQQLSEVSKFQSFGMPNWECDE 360
                 SILEQGLWDTMND ETVQSPIRPKI+RTPLQRIQQLSE+S  QS G PNWECDE
Sbjct: 301 TPSQRISILEQGLWDTMNDSETVQSPIRPKIHRTPLQRIQQLSEISTIQSSGTPNWECDE 360

Query: 361 DWVTVRSRGMGEEDREKNDFVSVLELPCSSESMAMTTKNEVDDQNYVSININRTYDCCSK 420
           DWVTVRS G+ EEDRE+N+FVSVLE+PCSS+S+     N   DQ+Y++++ ++  D CS+
Sbjct: 361 DWVTVRSIGLEEEDREENNFVSVLEMPCSSQSIERAINNGAVDQDYINVSDSKISDYCSR 420

Query: 421 KCSKAKKRDL-LMSSVSNQIVTFQTINLSNNDSLCIIISLFLVFPSSRSVYLIFLRYNDN 449
           K SK K RDL  MSSV NQIV+ +T  LSNN  LCIIISLFLVFP   S+ L FLRYN++
Sbjct: 421 KISKVKNRDLPAMSSVCNQIVSLKT-TLSNNHKLCIIISLFLVFPPRGSLDLTFLRYNED 480

BLAST of Spg011711 vs. NCBI nr
Match: XP_008441103.1 (PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo])

HSP 1 Score: 699.1 bits (1803), Expect = 2.6e-197
Identity = 350/454 (77.09%), Postives = 389/454 (85.68%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDFVHSDFLMREQRILSQLNCPNVVAY 60
           MEW RGR +G GSSAAVSVATDLRSGQ++AVK+V+F   DFL REQRILSQLNC  V+ Y
Sbjct: 1   MEWIRGRTLGRGSSAAVSVATDLRSGQVMAVKTVEFSRLDFLKREQRILSQLNCSRVIGY 60

Query: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREILNGLEYVHSNG 120
           KGFDVTLENGNLMCNLLMEFAPGGSILD ME AGGRLDEATAQ YTREIL+GL YVHSNG
Sbjct: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDTMEKAGGRLDEATAQFYTREILSGLHYVHSNG 120

Query: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVSGGNFAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCNILVGEDGIKIADFGCARRVEDV GGN AGTPIFMAPEVARGEKQGFAADV
Sbjct: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVFGGNLAGTPIFMAPEVARGEKQGFAADV 180

Query: 181 WAVGCTVIQMVTGGAPWADVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRDPE 240
           W+VGC VIQMVTG APWA++SDP+AAIYRIGSGDDLPEIPR MSEQGKDFLRRCLIRDPE
Sbjct: 181 WSVGCAVIQMVTGRAPWANLSDPLAAIYRIGSGDDLPEIPRNMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVSELLKHPFVQEQKFHPKQNSRTPTSILEQGLWDTMNDLETVQSPIRPKIYRTPLQ 300
           ERWSV+ELLKHPFVQEQK +PKQNSRTPTSILEQG+WDT+ND ETV+SPIRPKI+RTPLQ
Sbjct: 241 ERWSVNELLKHPFVQEQKSNPKQNSRTPTSILEQGIWDTVNDPETVESPIRPKIHRTPLQ 300

Query: 301 RIQQLSEVSKFQSFGMPNWECDEDWVTVRSRGMGEEDREKNDFVSVLELPCSSESMAMTT 360
           RIQQL+EVS       PNWECDEDW+TVRS G+     E+ND VSV+E PCSS+S+ M +
Sbjct: 301 RIQQLNEVST----NGPNWECDEDWITVRSIGL-----EENDIVSVMETPCSSQSVEMAS 360

Query: 361 KNEVDDQNYVSININRTYDCCSKKCSKAKKRDL-LMSSVSNQIVTFQTINLSNNDSLCII 420
            NEV DQ+Y++++ +R+ D CS+K SKAK RD   MSSV NQIV  +TIN SNN  LCII
Sbjct: 361 NNEVGDQDYINVSASRSSDYCSRKSSKAKNRDFPAMSSVFNQIVLLKTINFSNNHKLCII 420

Query: 421 ISLFLVFPSSRSVYLIFLRYNDNVLIYRDIDEIS 454
           ISLFLVFP+    YLI L YN+N  IY +  ++S
Sbjct: 421 ISLFLVFPARGLFYLIILSYNENTSIYHENTKMS 445

BLAST of Spg011711 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 4.6e-80
Identity = 162/356 (45.51%), Postives = 219/356 (61.52%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDFVHSDFLMREQRILSQLNCPNVVAY 60
           MEWTRGRI+G GS+A V  A    S ++LAVKS +   S+FL RE +ILS L+ P V+ Y
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  KGFDVTLE-NGNLMCNLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREILNGLEYVHSN 120
           +G +   E NG +M NLLME+AP G++ DA    GGR+DE     YTR+IL GLEY+HS 
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSK 120

Query: 121 GVVHCDIKCCNILVGEDG-IKIADFGCARRVEDVSGGNFAGTPIFMAPEVARGEKQGFAA 180
           G+VHCD+K  N+++ E G  KIADFGCA+RV+ V      GTP FMAPEVARGEKQG  +
Sbjct: 121 GIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPVMGTPAFMAPEVARGEKQGKES 180

Query: 181 DVWAVGCTVIQMVTGGAPWADV---SDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCL 240
           D+WAVGCT+I+MVTG  PW       DPV+ +YR+G   + PE+P  ++E+ KDFL +CL
Sbjct: 181 DIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLEKCL 240

Query: 241 IRDPEERWSVSELLKHPFVQ-----EQKFHPKQNSRTPTSILEQGLWDTM-----NDLET 300
            R+  ERW+ ++LL HPF+      E    P   S +PTS+ +Q  W ++      + E 
Sbjct: 241 KREANERWTATQLLNHPFLTTKPDIEPVLVPGLISNSPTSVTDQTFWRSVEEEEEEETEE 300

Query: 301 VQSPIRP----KIYRTPLQRIQQLSEVSKFQSFGMPNWECD---EDWVTVRSRGMG 335
           +Q   R      ++    +RI +L  V      G+    CD    DW+ VR+R  G
Sbjct: 301 IQKDSRDLDRLSLWGCYSERIGRLKCVG-----GLDGTRCDMEGGDWIMVRARCEG 351

BLAST of Spg011711 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.8e-76
Identity = 161/341 (47.21%), Postives = 217/341 (63.64%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDFVHSDFLMREQRILSQLNCPNVVAY 60
           M WTRG+ +G GS+A VS AT   SG+ LAVKS +F  S+FL RE +ILS LN P V+ Y
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGETLAVKSAEFHRSEFLQREAKILSSLNSPYVIGY 60

Query: 61  KGFDVTLE----NGNLMC-NLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREILNGLEY 120
           +G ++T E    NG     +LLME+AP G++ D     GG +DEA    YTR+IL GLEY
Sbjct: 61  RGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQILLGLEY 120

Query: 121 VH-SNGVVHCDIKCCNILVGEDG-IKIADFGCARRVEDVSGGNFAGTPIFMAPEVARGEK 180
           +H S G+ HCDIK  N+LVGE+G  KIADFGCA+ VE        GTP FMAPE ARGE+
Sbjct: 121 IHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPVRGTPAFMAPEAARGER 180

Query: 181 QGFAADVWAVGCTVIQMVTGGAPW--ADVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFL 240
           QG  +D+WAVGCTVI+MVTG  PW  AD +DPV+ +YR+G   +LPE+P  ++EQ KDFL
Sbjct: 181 QGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLTEQAKDFL 240

Query: 241 RRCLIRDPEERWSVSELLKHPFV--QEQKFHPKQNSRTPTSILEQGLWDTMNDLETVQSP 300
            +CL ++  ERW+ S+LL HPF+  +E +      + +PTS+ +Q  W ++ +  +    
Sbjct: 241 GKCLKKEATERWTASQLLNHPFLVNKEPELVTGLVTNSPTSVTDQMFWRSVEEEVSEDRS 300

Query: 301 IRPKIYRTPLQRIQQLSEVSKFQSFGMPNWECD-EDWVTVR 330
              + +    +RI  LS +          W+ D EDW+TVR
Sbjct: 301 SWWECHED--ERIGVLSWIG--HVVVESTWDLDGEDWITVR 337

BLAST of Spg011711 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 5.5e-57
Identity = 136/355 (38.31%), Postives = 197/355 (55.49%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSG----QLLAVKSVDFVHSDFLMREQRILSQL-NCP 60
           MEW RG  IG G+ + VS AT  R+      L+AVKS D   +  L  E+ +L  L +CP
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCP 60

Query: 61  NVVAYKGFDVTLENGNLMCNLLMEFAPGGSILDAMEGAGGR-LDEATAQLYTREILNGLE 120
            ++   G D T+ENG  M NLL+E+A  GS+   M+  GG  L E+T + +T  +L GL 
Sbjct: 61  EIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLR 120

Query: 121 YVHSNGVVHCDIKCCNILVGEDG-IKIADFGCARRVE-DVS----GGNFAGTPIFMAPEV 180
           ++H+ G  HCDIK  NIL+  DG +KIADFG A RV+ D++         GTP++MAPE 
Sbjct: 121 HIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPEC 180

Query: 181 ARGEKQGFAADVWAVGCTVIQMVTGGAPWA--DVSDPVAAIYRIGSGDDLPEIPRFMSEQ 240
               + G AADVWA+GC V++M +G   W+  + S  ++ + RIG GD+LP+IP  +SE+
Sbjct: 181 VNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEE 240

Query: 241 GKDFLRRCLIRDPEERWSVSELLKHPFV-------QEQKFHPKQNSR-----TPTSILEQ 300
           GKDFL +C ++DP +RW+   LL H FV         + F  K         +P    E 
Sbjct: 241 GKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCPFEF 300

Query: 301 GLWDTMNDLETVQSPIRPKIYRTPLQRIQQLSEVSKFQSFGMPNWECDEDWVTVR 330
             WD+   L++  S      + +P++R+  L       S  +P+W     W+TVR
Sbjct: 301 DDWDSFT-LDSNPS------FDSPVERLGSL------VSGSIPDWSVGGSWLTVR 342

BLAST of Spg011711 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 7.7e-51
Identity = 111/270 (41.11%), Postives = 161/270 (59.63%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDF-----------VHSDFLMREQRIL 60
           + W +G +IG G+   V +  ++ SG+LLA+K V              H   L  E  +L
Sbjct: 78  IRWRKGEMIGCGAFGRVYMGMNVDSGELLAIKEVSIAMNGASRERAQAHVRELEEEVNLL 137

Query: 61  SQLNCPNVVAYKGFDVTLENGNLMCNLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREI 120
             L+ PN+V Y G     E G+L  N+L+EF PGGSI  ++ G  G   E+  ++YT+++
Sbjct: 138 KNLSHPNIVRYLG--TAREAGSL--NILLEFVPGGSI-SSLLGKFGSFPESVIRMYTKQL 197

Query: 121 LNGLEYVHSNGVVHCDIKCCNILVGEDG-IKIADFGCARRVEDVS----GGNFAGTPIFM 180
           L GLEY+H NG++H DIK  NILV   G IK+ADFG +++V +++      +  GTP +M
Sbjct: 198 LLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYWM 257

Query: 181 APEVARGEKQGFAADVWAVGCTVIQMVTGGAPWADVSDPVAAIYRIGSGDDLPEIPRFMS 240
           APEV       F+AD+W+VGCT+I+M TG  PW+     VAA++ IG+    P IP  +S
Sbjct: 258 APEVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEHLS 317

Query: 241 EQGKDFLRRCLIRDPEERWSVSELLKHPFV 255
            + KDFL +CL ++P  R S S LL+HPFV
Sbjct: 318 AESKDFLLKCLQKEPHLRHSASNLLQHPFV 342

BLAST of Spg011711 vs. ExPASy Swiss-Prot
Match: O22040 (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3702 GN=ANP1 PE=1 SV=2)

HSP 1 Score: 202.6 bits (514), Expect = 1.0e-50
Identity = 133/389 (34.19%), Postives = 208/389 (53.47%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSV-----------DFVHSDFLMREQRIL 60
           + W +G++IG G+   V +  +L SG+LLAVK V              H   L  E ++L
Sbjct: 67  ISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLL 126

Query: 61  SQLNCPNVVAYKGFDVTLENGNLMCNLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREI 120
             L+ PN+V Y G   T+   + + N+L+EF PGGSI   +E   G   E+  + YTR++
Sbjct: 127 KNLSHPNIVRYLG---TVREDDTL-NILLEFVPGGSISSLLE-KFGPFPESVVRTYTRQL 186

Query: 121 LNGLEYVHSNGVVHCDIKCCNILVGEDG-IKIADFGCARRVEDVS----GGNFAGTPIFM 180
           L GLEY+H++ ++H DIK  NILV   G IK+ADFG +++V +++      +  GTP +M
Sbjct: 187 LLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPYWM 246

Query: 181 APEVARGEKQGFAADVWAVGCTVIQMVTGGAPWADVSDPVAAIYRIGSGDDLPEIPRFMS 240
           APEV       F+AD+W+VGCTVI+MVTG APW+     VAAI+ IG+    P IP  +S
Sbjct: 247 APEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTLS 306

Query: 241 EQGKDFLRRCLIRDPEERWSVSELLKHPFVQEQKFHPKQNSRTPTSILEQGLWDTMNDLE 300
              KDFL +CL   P  R + SELLKHPFV  +    K+++ T        L   +N+L 
Sbjct: 307 SDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKH---KESAST-------DLGSVLNNLS 366

Query: 301 TVQSPIRPKIYRTPLQRIQQLSEVSKFQSFGMPNWECDEDWVTVRSRGMGEEDREKNDFV 360
           T   P+  +I  T         +V    +FG  N+   +   +++++ + +++    D  
Sbjct: 367 T---PLPLQINNTKSTPDSTCDDVGDMCNFGSLNYSLVDPVKSIQNKNLWQQNDNGGD-- 422

Query: 361 SVLELPCSSESMAMTTKNEVDDQNYVSIN 374
                    + M +     +DD+N+++ +
Sbjct: 427 --------EDDMCL-----IDDENFLTFD 422

BLAST of Spg011711 vs. ExPASy TrEMBL
Match: A0A6J1EMC5 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111433865 PE=3 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 7.1e-201
Identity = 362/453 (79.91%), Postives = 390/453 (86.09%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDFVHSDFLMREQRILSQLNCPNVVAY 60
           MEWTRGR+IG GSSAAVSVATDLRSGQLLAVKSV+F  SDF+ REQRILSQLN   V+A 
Sbjct: 13  MEWTRGRVIGCGSSAAVSVATDLRSGQLLAVKSVEFDRSDFIKREQRILSQLNSSRVIAC 72

Query: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREILNGLEYVHSNG 120
           KGFDVTLENGNLMCNLLMEFA GGSILDAME AGGRLDEATAQ YTREIL+GLEYVHSNG
Sbjct: 73  KGFDVTLENGNLMCNLLMEFASGGSILDAMEAAGGRLDEATAQFYTREILSGLEYVHSNG 132

Query: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVSGGNFAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKC NILVGEDGIKIADFGCARRVE+VSGGN AGTPIFMAPEVARGEKQGFAADV
Sbjct: 133 VVHCDIKCSNILVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 192

Query: 181 WAVGCTVIQMVTGGAPWADVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRDPE 240
           WAVGCTVIQMVTG APW D+SDPVAAIYRIGS DDLPEIPRFMSEQGK+FLRRCLIRDPE
Sbjct: 193 WAVGCTVIQMVTGRAPWEDMSDPVAAIYRIGSSDDLPEIPRFMSEQGKEFLRRCLIRDPE 252

Query: 241 ERWSVSELLKHPFVQEQKFHPKQNSRTPTSILEQGLWDTMNDLETVQSPIRPKIYRTPLQ 300
           ERWSVSELLKHPFV EQK  PKQN RTPTSILEQ LW+TMNDLETVQSPIRPK YRTPLQ
Sbjct: 253 ERWSVSELLKHPFVHEQKSRPKQNPRTPTSILEQELWNTMNDLETVQSPIRPKFYRTPLQ 312

Query: 301 RIQQLSEVSKFQSFGMPNWECDEDWVTVRSRGM--GEEDREKNDFVSVLELPCSSESMAM 360
           RIQQLSEVSKFQSFG+PNWE +EDWVTVRS+ M   EEDREK DF+S LELPCSSESMA 
Sbjct: 313 RIQQLSEVSKFQSFGIPNWESNEDWVTVRSKDMEEEEEDREKRDFISELELPCSSESMAT 372

Query: 361 TTKNEVDDQNYVSININRTYDCCSKKCSKAKKRDLLMSSVSNQIVTFQTINLSNNDSLCI 420
           TT+N   DQNY+SI+ +  Y CC       KK +  MSSV +QIV+   +N  NN+ LC 
Sbjct: 373 TTQNGAGDQNYISISKSSDY-CC-------KKINFPMSSVFSQIVSLNIVNFCNNNKLCY 432

Query: 421 IISLFLVFPSSRSVYLIFLRYNDNVLIYRDIDE 452
           +ISL LVF S+ SV+L   RYN+ +LIY +IDE
Sbjct: 433 VISLLLVFLSASSVHLTSFRYNEFILIYHNIDE 457

BLAST of Spg011711 vs. ExPASy TrEMBL
Match: A0A6J1KWJ0 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=3661 GN=LOC111497058 PE=3 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 4.6e-200
Identity = 362/455 (79.56%), Postives = 390/455 (85.71%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDFVHSDFLMREQRILSQLNCPNVVAY 60
           MEWTRGR+IG GSSAAVSVATDLRSGQLLAVKSV+F  SDFL REQRILSQLN   V+A 
Sbjct: 1   MEWTRGRVIGCGSSAAVSVATDLRSGQLLAVKSVEFDRSDFLKREQRILSQLNSSRVIAC 60

Query: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREILNGLEYVHSNG 120
           KGFDVTLENGNLMCNLLMEFA GGSILDAME AGGRLDEATAQ YTRE+L+GLEYVHSNG
Sbjct: 61  KGFDVTLENGNLMCNLLMEFASGGSILDAMEAAGGRLDEATAQFYTREVLSGLEYVHSNG 120

Query: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVSGGNFAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKC NILVGEDGIKIADFGCARRVE+VS GN AGTPIFMAPEVARGEKQGFAADV
Sbjct: 121 VVHCDIKCSNILVGEDGIKIADFGCARRVEEVSCGNLAGTPIFMAPEVARGEKQGFAADV 180

Query: 181 WAVGCTVIQMVTGGAPWADVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRDPE 240
           WAVGCTVIQMVTG APW D+SDPVAAIYRIGS DDLPEIPRFMSEQGKDFLRRCLIRDPE
Sbjct: 181 WAVGCTVIQMVTGRAPWEDLSDPVAAIYRIGSSDDLPEIPRFMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVSELLKHPFVQEQKFHPKQNSRTPTSILEQGLWDTMNDLETVQSPIRPKIYRTPLQ 300
           ERWS+SELLKHPFV EQK  PKQN RTPTSILEQGLW+TMNDL TVQSPIRPK YRTPLQ
Sbjct: 241 ERWSLSELLKHPFVHEQKSRPKQNPRTPTSILEQGLWNTMNDLGTVQSPIRPKFYRTPLQ 300

Query: 301 RIQQLSEVSKFQSFGMPNWECDEDWVTVRSRGM----GEEDREKNDFVSVLELPCSSESM 360
           RIQQLSEVSKFQSFG+PNWE +EDWVTVRS+ M     EEDREK DF+S LELPCSSESM
Sbjct: 301 RIQQLSEVSKFQSFGIPNWESNEDWVTVRSKDMEEEEEEEDREKRDFISELELPCSSESM 360

Query: 361 AMTTKNEVDDQNYVSININRTYDCCSKKCSKAKKRDLLMSSVSNQIVTFQTINLSNNDSL 420
           A TT+N   DQNY+SI+ +  Y CC       KK +  MSSV +QIV+   IN  NN++L
Sbjct: 361 ATTTQNGAGDQNYISISESSDY-CC-------KKINFPMSSVFSQIVSLNFINFCNNNNL 420

Query: 421 CIIISLFLVFPSSRSVYLIFLRYNDNVLIYRDIDE 452
           C +ISL LVF S+ SV+L   RYN+ +LIY +IDE
Sbjct: 421 CYVISLLLVFLSASSVHLTSFRYNEFILIYHNIDE 447

BLAST of Spg011711 vs. ExPASy TrEMBL
Match: A0A1S3B3E3 (mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103485329 PE=3 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 1.3e-197
Identity = 350/454 (77.09%), Postives = 389/454 (85.68%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDFVHSDFLMREQRILSQLNCPNVVAY 60
           MEW RGR +G GSSAAVSVATDLRSGQ++AVK+V+F   DFL REQRILSQLNC  V+ Y
Sbjct: 1   MEWIRGRTLGRGSSAAVSVATDLRSGQVMAVKTVEFSRLDFLKREQRILSQLNCSRVIGY 60

Query: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREILNGLEYVHSNG 120
           KGFDVTLENGNLMCNLLMEFAPGGSILD ME AGGRLDEATAQ YTREIL+GL YVHSNG
Sbjct: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDTMEKAGGRLDEATAQFYTREILSGLHYVHSNG 120

Query: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVSGGNFAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCNILVGEDGIKIADFGCARRVEDV GGN AGTPIFMAPEVARGEKQGFAADV
Sbjct: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVFGGNLAGTPIFMAPEVARGEKQGFAADV 180

Query: 181 WAVGCTVIQMVTGGAPWADVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRDPE 240
           W+VGC VIQMVTG APWA++SDP+AAIYRIGSGDDLPEIPR MSEQGKDFLRRCLIRDPE
Sbjct: 181 WSVGCAVIQMVTGRAPWANLSDPLAAIYRIGSGDDLPEIPRNMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVSELLKHPFVQEQKFHPKQNSRTPTSILEQGLWDTMNDLETVQSPIRPKIYRTPLQ 300
           ERWSV+ELLKHPFVQEQK +PKQNSRTPTSILEQG+WDT+ND ETV+SPIRPKI+RTPLQ
Sbjct: 241 ERWSVNELLKHPFVQEQKSNPKQNSRTPTSILEQGIWDTVNDPETVESPIRPKIHRTPLQ 300

Query: 301 RIQQLSEVSKFQSFGMPNWECDEDWVTVRSRGMGEEDREKNDFVSVLELPCSSESMAMTT 360
           RIQQL+EVS       PNWECDEDW+TVRS G+     E+ND VSV+E PCSS+S+ M +
Sbjct: 301 RIQQLNEVST----NGPNWECDEDWITVRSIGL-----EENDIVSVMETPCSSQSVEMAS 360

Query: 361 KNEVDDQNYVSININRTYDCCSKKCSKAKKRDL-LMSSVSNQIVTFQTINLSNNDSLCII 420
            NEV DQ+Y++++ +R+ D CS+K SKAK RD   MSSV NQIV  +TIN SNN  LCII
Sbjct: 361 NNEVGDQDYINVSASRSSDYCSRKSSKAKNRDFPAMSSVFNQIVLLKTINFSNNHKLCII 420

Query: 421 ISLFLVFPSSRSVYLIFLRYNDNVLIYRDIDEIS 454
           ISLFLVFP+    YLI L YN+N  IY +  ++S
Sbjct: 421 ISLFLVFPARGLFYLIILSYNENTSIYHENTKMS 445

BLAST of Spg011711 vs. ExPASy TrEMBL
Match: A0A6J1GDW5 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111453267 PE=3 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 1.6e-189
Identity = 343/450 (76.22%), Postives = 380/450 (84.44%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDFVHSDFLMREQRILSQLNCPNVVAY 60
           MEWTRG  IG GSSAAVSVATDL SGQ+LAVKSV+F  SD L REQRILS+ NCP V+AY
Sbjct: 20  MEWTRGPTIGRGSSAAVSVATDLCSGQVLAVKSVEFTRSDCLKREQRILSRFNCPRVIAY 79

Query: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREILNGLEYVHSNG 120
           KGFDVTLENG LMCNLLMEFAPGGSILD ME AGGRLDEA AQ YTREIL+GLEYVH+NG
Sbjct: 80  KGFDVTLENGELMCNLLMEFAPGGSILDVMEAAGGRLDEARAQFYTREILSGLEYVHTNG 139

Query: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVSGGNFAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCNILVGEDGIKIADFGCAR+VEDVS  N AGT IFMAPEVARGEKQGFAADV
Sbjct: 140 VVHCDIKCCNILVGEDGIKIADFGCARQVEDVSCRNLAGTLIFMAPEVARGEKQGFAADV 199

Query: 181 WAVGCTVIQMVTGGAPWADVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRDPE 240
           WAVGC VIQMVTG APW +VSDPVAAIY+IGSGDDLPE+PRF+S+QGKDFLRRCLIRDPE
Sbjct: 200 WAVGCAVIQMVTGRAPWVNVSDPVAAIYKIGSGDDLPEVPRFISDQGKDFLRRCLIRDPE 259

Query: 241 ERWSVSELLKHPFVQEQKFHPKQNSRTPTSILEQGLWDTMNDLETVQSPIRPKIYRTPLQ 300
           ERWSVSELLKHPFV EQK  PKQNSRTPTSILEQ  W TMN+ ETVQ PI PK+YRTPLQ
Sbjct: 260 ERWSVSELLKHPFVHEQKSPPKQNSRTPTSILEQESWYTMNNSETVQIPIMPKLYRTPLQ 319

Query: 301 RIQQLSEVSKFQSFGMPNWECDEDWVTVRSRGMGEEDREKNDFVSVLELPCSSESMAMTT 360
           RIQQLSE+SK QS  +PNW+CD+DWVTV+S  M EED E+++FVSVLELPC S+S+ M T
Sbjct: 320 RIQQLSEISKIQSSRIPNWDCDKDWVTVKSITMEEEDTEQSEFVSVLELPCFSQSIKMAT 379

Query: 361 KNEVDDQNYVSININRTYDCCSKKCSKAKKRDL-LMSSVSNQIVTFQTINLSNNDSLCII 420
           KN V DQ Y++++I       S++ SKAKKRDL  MSSVSNQIV+  T + SNN  LCII
Sbjct: 380 KNGVGDQTYINVSI-------SRESSKAKKRDLPAMSSVSNQIVSLNTNSFSNNHKLCII 439

Query: 421 ISLFLVF-PSSRSVYLIFLRYNDNVLIYRD 449
            SLFLVF  S  S+ + FLRY +N+ IY +
Sbjct: 440 SSLFLVFLASGGSIRVTFLRYIENIWIYHE 462

BLAST of Spg011711 vs. ExPASy TrEMBL
Match: A0A5D3CNL0 (Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006310 PE=3 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 3.0e-183
Identity = 322/403 (79.90%), Postives = 354/403 (87.84%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDFVHSDFLMREQRILSQLNCPNVVAY 60
           MEW RGR +G GSSAAVSVATDLRSGQ++AVK+V+F   DFL REQRILSQLNC  V+ Y
Sbjct: 1   MEWIRGRTLGRGSSAAVSVATDLRSGQVMAVKTVEFSRLDFLKREQRILSQLNCSRVIGY 60

Query: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREILNGLEYVHSNG 120
           KGFDVTLENGNLMCNLLMEFAPGGSILD ME AGGRLDEATAQ YTREIL+GL YVHSNG
Sbjct: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDTMEKAGGRLDEATAQFYTREILSGLHYVHSNG 120

Query: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVSGGNFAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCNILVGEDGIKIADFGCARRVEDV GGN AGTPIFMAPEVARGEKQGFAADV
Sbjct: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVFGGNLAGTPIFMAPEVARGEKQGFAADV 180

Query: 181 WAVGCTVIQMVTGGAPWADVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRDPE 240
           W+VGC VIQMVTG APWA++SDP+AAIYRIGSGDDLPEIPR MSEQGKDFLRRCLIRDPE
Sbjct: 181 WSVGCAVIQMVTGRAPWANLSDPLAAIYRIGSGDDLPEIPRNMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVSELLKHPFVQEQKFHPKQNSRTPTSILEQGLWDTMNDLETVQSPIRPKIYRTPLQ 300
           ERWSV+ELLKHPFVQEQK HPKQNSRTPTSILEQG+WDT+ND ETV+SPIRPKI+RTPLQ
Sbjct: 241 ERWSVNELLKHPFVQEQKSHPKQNSRTPTSILEQGIWDTVNDPETVESPIRPKIHRTPLQ 300

Query: 301 RIQQLSEVSKFQSFGMPNWECDEDWVTVRSRGMGEEDREKNDFVSVLELPCSSESMAMTT 360
           RIQQL+EVS       PNWECDEDW+TVRS G+     E+ND VSV+E PCSS+S+ M +
Sbjct: 301 RIQQLNEVSTIG----PNWECDEDWITVRSIGL-----EENDIVSVMETPCSSQSVEMAS 360

Query: 361 KNEVDDQNYVSININRTYDCCSKKCSKAKKRDL-LMSSVSNQI 403
            NEV DQ+Y++++ +R+ D CS+K SKAK RD   MSSV NQI
Sbjct: 361 NNEVGDQDYINVSASRSSDYCSRKSSKAKNRDFPAMSSVFNQI 394

BLAST of Spg011711 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 308.1 bits (788), Expect = 1.2e-83
Identity = 162/371 (43.67%), Postives = 229/371 (61.73%), Query Frame = 0

Query: 3   WTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDFVHSDFLMREQRILSQLNCPNVVAYKG 62
           W RG IIG GS+A VS+     SG   AVKS +F  S FL REQ ILS+L+ P +V Y G
Sbjct: 6   WIRGPIIGRGSTATVSLGI-TNSGDFFAVKSAEFSSSAFLQREQSILSKLSSPYIVKYIG 65

Query: 63  FDVTLENGNLMCNLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREILNGLEYVHSNGVV 122
            +VT EN  LM NLLME+  GGS+ D ++ +GG+L E   + YTR+IL GL Y+H  G+V
Sbjct: 66  SNVTKENDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMYLHDQGIV 125

Query: 123 HCDIKCCNILVGEDGIKIADFGCARRVEDVSGGNFAGTPIFMAPEVARGEKQGFAADVWA 182
           HCD+K  N+++G +  KI D GCA+ VE+     F+GTP FM+PEVARGE+Q F ADVWA
Sbjct: 126 HCDVKSQNVMIGGEIAKIVDLGCAKTVEENENLEFSGTPAFMSPEVARGEEQSFPADVWA 185

Query: 183 VGCTVIQMVTGGAPWADVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRDPEER 242
           +GCTVI+M TG +PW +++D VAAIY+IG   + P IP ++SE+G+DFLR+CL +DP++R
Sbjct: 186 LGCTVIEMATGSSPWPELNDVVAAIYKIGFTGESPVIPVWLSEKGQDFLRKCLRKDPKQR 245

Query: 243 WSVSELLKHPFVQEQKFHPKQ-----NSRTPTSILEQGLWDTMNDLETVQSPIRPKIYRT 302
           W+V ELL+HPF+ E+     Q     NS +P+++L+Q  WD     ET +S    + +  
Sbjct: 246 WTVEELLQHPFLDEEDNDSDQTGNCLNSSSPSTVLDQRFWDL---CETSRSRFIKEDHED 305

Query: 303 PLQRI------------QQLSEVSKFQSFGMPNWECDEDWVTVRSRGMGEEDREKNDFVS 357
           P                 ++ +++  +S G P+WE +  W+ VR       + E  + V 
Sbjct: 306 PFANSTNFLWDDDSLPGDRIKKLAGDESSGEPDWETN-GWIEVRGEIEKRNEEEDENCVE 365

BLAST of Spg011711 vs. TAIR 10
Match: AT2G32510.1 (mitogen-activated protein kinase kinase kinase 17 )

HSP 1 Score: 300.1 bits (767), Expect = 3.3e-81
Identity = 162/356 (45.51%), Postives = 219/356 (61.52%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDFVHSDFLMREQRILSQLNCPNVVAY 60
           MEWTRGRI+G GS+A V  A    S ++LAVKS +   S+FL RE +ILS L+ P V+ Y
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  KGFDVTLE-NGNLMCNLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREILNGLEYVHSN 120
           +G +   E NG +M NLLME+AP G++ DA    GGR+DE     YTR+IL GLEY+HS 
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSK 120

Query: 121 GVVHCDIKCCNILVGEDG-IKIADFGCARRVEDVSGGNFAGTPIFMAPEVARGEKQGFAA 180
           G+VHCD+K  N+++ E G  KIADFGCA+RV+ V      GTP FMAPEVARGEKQG  +
Sbjct: 121 GIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPVMGTPAFMAPEVARGEKQGKES 180

Query: 181 DVWAVGCTVIQMVTGGAPWADV---SDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCL 240
           D+WAVGCT+I+MVTG  PW       DPV+ +YR+G   + PE+P  ++E+ KDFL +CL
Sbjct: 181 DIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLEKCL 240

Query: 241 IRDPEERWSVSELLKHPFVQ-----EQKFHPKQNSRTPTSILEQGLWDTM-----NDLET 300
            R+  ERW+ ++LL HPF+      E    P   S +PTS+ +Q  W ++      + E 
Sbjct: 241 KREANERWTATQLLNHPFLTTKPDIEPVLVPGLISNSPTSVTDQTFWRSVEEEEEEETEE 300

Query: 301 VQSPIRP----KIYRTPLQRIQQLSEVSKFQSFGMPNWECD---EDWVTVRSRGMG 335
           +Q   R      ++    +RI +L  V      G+    CD    DW+ VR+R  G
Sbjct: 301 IQKDSRDLDRLSLWGCYSERIGRLKCVG-----GLDGTRCDMEGGDWIMVRARCEG 351

BLAST of Spg011711 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 296.2 bits (757), Expect = 4.7e-80
Identity = 159/380 (41.84%), Postives = 241/380 (63.42%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDFVHSDFLMREQRILSQLNCPNVVAY 60
           + WTRG IIG GS+A VS+A    SG+L AVKS D   S  L +EQ ILS L+ P++V Y
Sbjct: 3   INWTRGPIIGRGSTATVSIAIS-SSGELFAVKSADLSSSSLLQKEQSILSTLSSPHMVKY 62

Query: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREILNGLEYVHSNG 120
            G  +T E+  L+ N+LME+  GG++ D ++ +GG+L E   + YTR+ILNGL Y+H  G
Sbjct: 63  IGTGLTRESNGLVYNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTRQILNGLVYLHERG 122

Query: 121 VVHCDIKCCNILVGEDGI-KIADFGCARRVEDVSGGNFAGTPIFMAPEVARGEKQGFAAD 180
           +VHCD+K  N+LV E+G+ KIAD GCA+ V+      F+GTP FMAPEVARGE+Q F AD
Sbjct: 123 IVHCDLKSHNVLVEENGVLKIADMGCAKSVDK---SEFSGTPAFMAPEVARGEEQRFPAD 182

Query: 181 VWAVGCTVIQMVTGGAPWADVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRDP 240
           VWA+GCT+I+M+TG +PW +++D VAA+Y+IG   + P IP ++S++ KDFL+ CL  D 
Sbjct: 183 VWALGCTMIEMMTGSSPWPELNDVVAAMYKIGFSGESPAIPAWISDKAKDFLKNCLKEDQ 242

Query: 241 EERWSVSELLKHPFVQEQKFHP-----KQNSRTPTSILEQGLWDTMNDLETVQSPI---- 300
           ++RW+V ELLKHPF+ + +        K  + +P+++L+Q  WD+    ++    I    
Sbjct: 243 KQRWTVEELLKHPFLDDDEESQTSDCLKNKTSSPSTVLDQRFWDSCESSKSHLVSIDHED 302

Query: 301 ----RPKIYRTPLQRIQQLSEVSKFQSFGMPNWECDEDWVTVRSRGMGEEDREKNDFVSV 360
                 +   +P  RI++L+   +F S  + +W+ ++D   ++ RG   ++ EK D    
Sbjct: 303 PFAEYSESLDSPADRIEKLAG-DEFSS--LLDWDTEDDGGWIQVRGEKHKETEKRD--GD 362

Query: 361 LELPCSSESMAMTTKNEVDD 367
            ++ C   + +  T  EV+D
Sbjct: 363 EDVICVEATSSSQTIEEVED 373

BLAST of Spg011711 vs. TAIR 10
Match: AT1G05100.1 (mitogen-activated protein kinase kinase kinase 18 )

HSP 1 Score: 288.1 bits (736), Expect = 1.3e-77
Identity = 161/341 (47.21%), Postives = 217/341 (63.64%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSGQLLAVKSVDFVHSDFLMREQRILSQLNCPNVVAY 60
           M WTRG+ +G GS+A VS AT   SG+ LAVKS +F  S+FL RE +ILS LN P V+ Y
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGETLAVKSAEFHRSEFLQREAKILSSLNSPYVIGY 60

Query: 61  KGFDVTLE----NGNLMC-NLLMEFAPGGSILDAMEGAGGRLDEATAQLYTREILNGLEY 120
           +G ++T E    NG     +LLME+AP G++ D     GG +DEA    YTR+IL GLEY
Sbjct: 61  RGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQILLGLEY 120

Query: 121 VH-SNGVVHCDIKCCNILVGEDG-IKIADFGCARRVEDVSGGNFAGTPIFMAPEVARGEK 180
           +H S G+ HCDIK  N+LVGE+G  KIADFGCA+ VE        GTP FMAPE ARGE+
Sbjct: 121 IHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPVRGTPAFMAPEAARGER 180

Query: 181 QGFAADVWAVGCTVIQMVTGGAPW--ADVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFL 240
           QG  +D+WAVGCTVI+MVTG  PW  AD +DPV+ +YR+G   +LPE+P  ++EQ KDFL
Sbjct: 181 QGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLTEQAKDFL 240

Query: 241 RRCLIRDPEERWSVSELLKHPFV--QEQKFHPKQNSRTPTSILEQGLWDTMNDLETVQSP 300
            +CL ++  ERW+ S+LL HPF+  +E +      + +PTS+ +Q  W ++ +  +    
Sbjct: 241 GKCLKKEATERWTASQLLNHPFLVNKEPELVTGLVTNSPTSVTDQMFWRSVEEEVSEDRS 300

Query: 301 IRPKIYRTPLQRIQQLSEVSKFQSFGMPNWECD-EDWVTVR 330
              + +    +RI  LS +          W+ D EDW+TVR
Sbjct: 301 SWWECHED--ERIGVLSWIG--HVVVESTWDLDGEDWITVR 337

BLAST of Spg011711 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 223.4 bits (568), Expect = 3.9e-58
Identity = 136/355 (38.31%), Postives = 197/355 (55.49%), Query Frame = 0

Query: 1   MEWTRGRIIGSGSSAAVSVATDLRSG----QLLAVKSVDFVHSDFLMREQRILSQL-NCP 60
           MEW RG  IG G+ + VS AT  R+      L+AVKS D   +  L  E+ +L  L +CP
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCP 60

Query: 61  NVVAYKGFDVTLENGNLMCNLLMEFAPGGSILDAMEGAGGR-LDEATAQLYTREILNGLE 120
            ++   G D T+ENG  M NLL+E+A  GS+   M+  GG  L E+T + +T  +L GL 
Sbjct: 61  EIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLR 120

Query: 121 YVHSNGVVHCDIKCCNILVGEDG-IKIADFGCARRVE-DVS----GGNFAGTPIFMAPEV 180
           ++H+ G  HCDIK  NIL+  DG +KIADFG A RV+ D++         GTP++MAPE 
Sbjct: 121 HIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPEC 180

Query: 181 ARGEKQGFAADVWAVGCTVIQMVTGGAPWA--DVSDPVAAIYRIGSGDDLPEIPRFMSEQ 240
               + G AADVWA+GC V++M +G   W+  + S  ++ + RIG GD+LP+IP  +SE+
Sbjct: 181 VNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEE 240

Query: 241 GKDFLRRCLIRDPEERWSVSELLKHPFV-------QEQKFHPKQNSR-----TPTSILEQ 300
           GKDFL +C ++DP +RW+   LL H FV         + F  K         +P    E 
Sbjct: 241 GKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCPFEF 300

Query: 301 GLWDTMNDLETVQSPIRPKIYRTPLQRIQQLSEVSKFQSFGMPNWECDEDWVTVR 330
             WD+   L++  S      + +P++R+  L       S  +P+W     W+TVR
Sbjct: 301 DDWDSFT-LDSNPS------FDSPVERLGSL------VSGSIPDWSVGGSWLTVR 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022926885.11.5e-20079.91mitogen-activated protein kinase kinase kinase 18-like [Cucurbita moschata][more]
XP_023518154.14.3e-20079.91mitogen-activated protein kinase kinase kinase 18-like [Cucurbita pepo subsp. pe... [more]
XP_023003448.19.5e-20079.56mitogen-activated protein kinase kinase kinase 18-like [Cucurbita maxima][more]
XP_038883085.12.4e-19874.07mitogen-activated protein kinase kinase kinase 18-like [Benincasa hispida][more]
XP_008441103.12.6e-19777.09PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
O808884.6e-8045.51Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q9ZVP51.8e-7647.21Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370... [more]
Q9SND65.5e-5738.31Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
Q405417.7e-5141.11Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097... [more]
O220401.0e-5034.19Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1EMC57.1e-20179.91mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=... [more]
A0A6J1KWJ04.6e-20079.56mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=36... [more]
A0A1S3B3E31.3e-19777.09mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN... [more]
A0A6J1GDW51.6e-18976.22mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=... [more]
A0A5D3CNL03.0e-18379.90Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuw... [more]
Match NameE-valueIdentityDescription
AT5G55090.11.2e-8343.67mitogen-activated protein kinase kinase kinase 15 [more]
AT2G32510.13.3e-8145.51mitogen-activated protein kinase kinase kinase 17 [more]
AT4G26890.14.7e-8041.84mitogen-activated protein kinase kinase kinase 16 [more]
AT1G05100.11.3e-7747.21mitogen-activated protein kinase kinase kinase 18 [more]
AT3G50310.13.9e-5838.31mitogen-activated protein kinase kinase kinase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 3..254
e-value: 6.0E-77
score: 271.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 4..254
e-value: 1.6E-53
score: 181.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 3..254
score: 41.290375
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 2..313
e-value: 7.6E-71
score: 241.0
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 1..357
NoneNo IPR availablePANTHERPTHR48011:SF6MAP KINASE KINASE KINASEcoord: 1..357
NoneNo IPR availableCDDcd06606STKc_MAPKKKcoord: 2..254
e-value: 9.83452E-141
score: 402.669
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 121..133
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 9..32
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 3..305

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg011711.1Spg011711.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity