Spg011178 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg011178
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	cytochrome P450, family 705, subfamily A, polypeptide 3
Location	scaffold5: 7937647 .. 7938267 (+)
RNA-Seq Expression	Spg011178
Synteny	Spg011178

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGGCACCGACACGACAGCGACGGCGGTGTTATGGGCGATGGGGGAGCTCTTGAATCGTCCCGAAGCAATCAAAAAGCTAAGAAAAGAGATCATTTCAGTGGTTGGAAACAAAAAGCTAGTCCAAGAATCGGACTTCCCCAAGCTCCCATATCTCCGAGCAGTGGTCAACGAAACCCTCCGCCTCCACCCGTCGGCGCCAATCATCATCAGAGAATGTTTAGATGACTGCAACATCAACGGTTCACTAGTCAAGGCAAAAACTCGAGTTTTGGTCAACGCTTACGCCGTCATGAGGGACCCAGAGTCCTGGTCTGAGCCCGACAAGTTCTTGCCGGAGAGGTTTCTGGAAGGGTCATCGGAGAACATTGGCAACCATCGGATGGAGATGAAGGGACAGAATTTTCGATACATTCCATTTGGGAGTGGGAAGAGAGGCTGCCCTGGTTCTTCTCTTGCCTTGTTGGTTTTTCCATGTGCAATTGCAGCCATGGTGCAGCGGTTTGATTGGAACATGGACGGCGATGGCGGCGTTGATTTAAAACCTGGGTCGGGATTTGCTGCAGAGATGGCGACACCGCTTGTCTGTTATGCAAACCCTATTCATGGGACTGATTAA

mRNA sequence

ATGGCAGGCACCGACACGACAGCGACGGCGGTGTTATGGGCGATGGGGGAGCTCTTGAATCGTCCCGAAGCAATCAAAAAGCTAAGAAAAGAGATCATTTCAGTGGTTGGAAACAAAAAGCTAGTCCAAGAATCGGACTTCCCCAAGCTCCCATATCTCCGAGCAGTGGTCAACGAAACCCTCCGCCTCCACCCGTCGGCGCCAATCATCATCAGAGAATGTTTAGATGACTGCAACATCAACGGTTCACTAGTCAAGGCAAAAACTCGAGTTTTGGTCAACGCTTACGCCGTCATGAGGGACCCAGAGTCCTGGTCTGAGCCCGACAAGTTCTTGCCGGAGAGGTTTCTGGAAGGGTCATCGGAGAACATTGGCAACCATCGGATGGAGATGAAGGGACAGAATTTTCGATACATTCCATTTGGGAGTGGGAAGAGAGGCTGCCCTGGTTCTTCTCTTGCCTTGTTGGTTTTTCCATGTGCAATTGCAGCCATGGTGCAGCGGTTTGATTGGAACATGGACGGCGATGGCGGCGTTGATTTAAAACCTGGGTCGGGATTTGCTGCAGAGATGGCGACACCGCTTGTCTGTTATGCAAACCCTATTCATGGGACTGATTAA

Coding sequence (CDS)

ATGGCAGGCACCGACACGACAGCGACGGCGGTGTTATGGGCGATGGGGGAGCTCTTGAATCGTCCCGAAGCAATCAAAAAGCTAAGAAAAGAGATCATTTCAGTGGTTGGAAACAAAAAGCTAGTCCAAGAATCGGACTTCCCCAAGCTCCCATATCTCCGAGCAGTGGTCAACGAAACCCTCCGCCTCCACCCGTCGGCGCCAATCATCATCAGAGAATGTTTAGATGACTGCAACATCAACGGTTCACTAGTCAAGGCAAAAACTCGAGTTTTGGTCAACGCTTACGCCGTCATGAGGGACCCAGAGTCCTGGTCTGAGCCCGACAAGTTCTTGCCGGAGAGGTTTCTGGAAGGGTCATCGGAGAACATTGGCAACCATCGGATGGAGATGAAGGGACAGAATTTTCGATACATTCCATTTGGGAGTGGGAAGAGAGGCTGCCCTGGTTCTTCTCTTGCCTTGTTGGTTTTTCCATGTGCAATTGCAGCCATGGTGCAGCGGTTTGATTGGAACATGGACGGCGATGGCGGCGTTGATTTAAAACCTGGGTCGGGATTTGCTGCAGAGATGGCGACACCGCTTGTCTGTTATGCAAACCCTATTCATGGGACTGATTAA

Protein sequence

MAGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNETLRLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGSSENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGDGGVDLKPGSGFAAEMATPLVCYANPIHGTD

Homology

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BLAST of Spg011178 vs. NCBI nr
Match: XP_038905529.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Benincasa hispida])

HSP 1 Score: 375.2 bits (962), Expect = 3.7e-100
Identity = 176/208 (84.62%), Postives = 192/208 (92.31%), Query Frame = 0


Query: 1   MAGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNET 60
           MAGTDTTATA+LWAMGELLNR E++++LRKEI SV+GNKK+V+ESD P LPYLRAVVNET
Sbjct: 335 MAGTDTTATAILWAMGELLNRRESLQELRKEITSVIGNKKMVEESDLPNLPYLRAVVNET 394

Query: 61  LRLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGS 120
           LRLHPSAPIIIRECLDDCNING L+KAKTRVLVNAYAVM+DPESWS+PDKFLPERFL+GS
Sbjct: 395 LRLHPSAPIIIRECLDDCNINGLLIKAKTRVLVNAYAVMKDPESWSDPDKFLPERFLKGS 454

Query: 121 SENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNM--DGDGG 180
            +NIG HRMEMKGQNFRYIPFGSGKRGCPG+SLALLVFPCAIA MVQRFDW    DG G 
Sbjct: 455 LDNIGTHRMEMKGQNFRYIPFGSGKRGCPGASLALLVFPCAIATMVQRFDWKTSGDGSGN 514

Query: 181 VDLKPGSGFAAEMATPLVCYANPIHGTD 207
           +DL PGSGFAAEMATPL CYANPIHG+D
Sbjct: 515 IDLTPGSGFAAEMATPLFCYANPIHGSD 542

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BLAST of Spg011178 vs. NCBI nr
Match: XP_022982904.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita maxima])

HSP 1 Score: 368.6 bits (945), Expect = 3.5e-98
Identity = 178/206 (86.41%), Postives = 192/206 (93.20%), Query Frame = 0


Query: 1   MAGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNET 60
           MAGTDTTATA+LWAM ELLNRP+++++LRKEIISVVG +KLVQESD P LPYLRAVVNET
Sbjct: 321 MAGTDTTATAILWAMAELLNRPKSMEELRKEIISVVGEEKLVQESDLPNLPYLRAVVNET 380

Query: 61  LRLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGS 120
           LRLHPSAP+IIRECLDDC INGSLVKAKTRVLVNAYAVMRDPESWSEPD+FLPERFLEGS
Sbjct: 381 LRLHPSAPMIIRECLDDCIINGSLVKAKTRVLVNAYAVMRDPESWSEPDRFLPERFLEGS 440

Query: 121 SENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGDGG-- 180
            ENIG+HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCA+AAMVQRFDW +DGDG   
Sbjct: 441 HENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAMAAMVQRFDWKVDGDGDGD 500

Query: 181 -VDLKPGSGFAAEMATPLVCYANPIH 204
            +DL PG GFAAEMATPLV YANPI+
Sbjct: 501 TIDLTPGPGFAAEMATPLVRYANPIN 526

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BLAST of Spg011178 vs. NCBI nr
Match: KAG7017572.1 (3,9-dihydroxypterocarpan 6A-monooxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 368.6 bits (945), Expect = 3.5e-98
Identity = 178/206 (86.41%), Postives = 192/206 (93.20%), Query Frame = 0


Query: 1   MAGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNET 60
           MAGTDTTATA+LWAM ELLNRP+++++LRKEIISVVG KKLVQESD P LPYLRAVVNET
Sbjct: 319 MAGTDTTATAILWAMAELLNRPKSMEELRKEIISVVGEKKLVQESDLPNLPYLRAVVNET 378

Query: 61  LRLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGS 120
           LRLHPSAP+IIRECLDDC INGSLVKAKTRVLVNAYAVMRDPESWSEPD+FLPERFLEGS
Sbjct: 379 LRLHPSAPLIIRECLDDCIINGSLVKAKTRVLVNAYAVMRDPESWSEPDRFLPERFLEGS 438

Query: 121 SENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGDGG-- 180
            ENIG+HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCA+AAMVQRFDW +DGDG   
Sbjct: 439 HENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAMAAMVQRFDWKVDGDGDGD 498

Query: 181 -VDLKPGSGFAAEMATPLVCYANPIH 204
            ++L PG GFAAEMATPLV YANPI+
Sbjct: 499 TINLTPGPGFAAEMATPLVRYANPIN 524

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BLAST of Spg011178 vs. NCBI nr
Match: XP_023528514.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 367.9 bits (943), Expect = 5.9e-98
Identity = 177/206 (85.92%), Postives = 193/206 (93.69%), Query Frame = 0


Query: 1   MAGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNET 60
           MAGTDTTATA+LWAM ELLNRP+++++LRKEIISVVG +KLVQESD P LPYLRAVVNET
Sbjct: 319 MAGTDTTATAILWAMAELLNRPKSMEELRKEIISVVGEEKLVQESDLPNLPYLRAVVNET 378

Query: 61  LRLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGS 120
           LRLHPSAP+IIRECLDDC INGSLVKAKTRVLVNAYAVMRDPESWSEPD+FLPERFLEGS
Sbjct: 379 LRLHPSAPLIIRECLDDCIINGSLVKAKTRVLVNAYAVMRDPESWSEPDRFLPERFLEGS 438

Query: 121 SENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGDGG-- 180
           +ENIG+HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCA+AAMVQRFDW +DGDG   
Sbjct: 439 NENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAMAAMVQRFDWKVDGDGDGN 498

Query: 181 -VDLKPGSGFAAEMATPLVCYANPIH 204
            +DL PG GFAAEMATPLV YA+PI+
Sbjct: 499 TIDLTPGPGFAAEMATPLVRYADPIN 524

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BLAST of Spg011178 vs. NCBI nr
Match: KAA0043713.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Cucumis melo var. makuwa] >TYK25421.1 3,9-dihydroxypterocarpan 6A-monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 367.1 bits (941), Expect = 1.0e-97
Identity = 178/204 (87.25%), Postives = 188/204 (92.16%), Query Frame = 0


Query: 1   MAGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNET 60
           MAGTDTTATA+LWAMGELLN PE+++KLRKEI SVVGNKK VQESD P LPYLRA+VNET
Sbjct: 318 MAGTDTTATAMLWAMGELLNCPESLQKLRKEITSVVGNKKPVQESDLPNLPYLRAIVNET 377

Query: 61  LRLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGS 120
           LRLHPSAPIIIRECLDDCNIN SL+KAKTRVL+NAYAVMRDPESWSEPDKFLPERFLEGS
Sbjct: 378 LRLHPSAPIIIRECLDDCNINDSLIKAKTRVLINAYAVMRDPESWSEPDKFLPERFLEGS 437

Query: 121 SENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGD--GG 180
            ENIG+HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFP AIA MVQRFDW + GD  G 
Sbjct: 438 HENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPRAIATMVQRFDWKIGGDNGGN 497

Query: 181 VDLKPGSGFAAEMATPLVCYANPI 203
           VDL  GSGFAAEMATPL+CYANPI
Sbjct: 498 VDLTLGSGFAAEMATPLICYANPI 521

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BLAST of Spg011178 vs. ExPASy Swiss-Prot
Match: Q42798 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 8.1e-50
Identity = 90/202 (44.55%), Postives = 136/202 (67.33%), Query Frame = 0


Query: 1   MAGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNET 60
           +AGTDT+A ++ WAM EL+N P+ ++K R+EI +VVG  ++V+ESD   LPYL+A+V ET
Sbjct: 305 VAGTDTSAVSIEWAMAELINNPDVLEKARQEIDAVVGKSRMVEESDIANLPYLQAIVRET 364

Query: 61  LRLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGS 120
           LRLHP  P+++RE      + G  + AKTR+ VN +A+ RDP  W +P +F PERF+   
Sbjct: 365 LRLHPGGPLVVRESSKSAVVCGYDIPAKTRLFVNVWAIGRDPNHWEKPFEFRPERFIRD- 424

Query: 121 SENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNM-DGDGGV 180
               G ++++++GQ++ +IPFGSG+R CPG+SLA  V P  +A ++Q F W +  G+G V
Sbjct: 425 ----GQNQLDVRGQHYHFIPFGSGRRTCPGASLAWQVVPVNLAIIIQCFQWKLVGGNGKV 484

Query: 181 DLKPGSGFAAEMATPLVCYANP 202
           D++  SG     A P++C   P
Sbjct: 485 DMEEKSGITLPRANPIICVPVP 501

[Close]

BLAST of Spg011178 vs. ExPASy Swiss-Prot
Match: Q42799 (Cytochrome P450 93A2 OS=Glycine max OX=3847 GN=CYP93A2 PE=2 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 4.0e-49
Identity = 91/202 (45.05%), Postives = 132/202 (65.35%), Query Frame = 0


Query: 1   MAGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNET 60
           +AGTDT+A  + WAM EL+N P  ++K R+EI +VVGN ++++ESD   LPYL+A+V ET
Sbjct: 298 VAGTDTSAATMEWAMAELINNPCVLEKARQEIDAVVGNSRIIEESDIVNLPYLQAIVRET 357

Query: 61  LRLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGS 120
           LR+HP  P+I+RE      + G  + AKTR+ VN +A+ RDP  W  P +F PERF E  
Sbjct: 358 LRIHPGGPLIVRESSKSVVVCGYEIPAKTRLFVNVWAIGRDPNHWENPFEFRPERFFEN- 417

Query: 121 SENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMD-GDGGV 180
               G  +++++GQ++ +IPFGSG+R CPG+SLAL +    +A M+Q F W  D G+  V
Sbjct: 418 ----GQSQLDVRGQHYHFIPFGSGRRSCPGTSLALQIVHVNLAIMIQCFQWKFDNGNNKV 477

Query: 181 DLKPGSGFAAEMATPLVCYANP 202
           D++  SG     A P++C   P
Sbjct: 478 DMEEKSGITLPRAHPIICVPVP 494

[Close]

BLAST of Spg011178 vs. ExPASy Swiss-Prot
Match: Q0WQ07 (Cytochrome P450 705A1 OS=Arabidopsis thaliana OX=3702 GN=CYP705A1 PE=2 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 9.9e-48
Identity = 93/205 (45.37%), Postives = 128/205 (62.44%), Query Frame = 0


Query: 1   MAGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNET 60
           +   D ++ A+ WAM +++N  E ++KLR+EI SVVG  +LVQE+D P LPYL+AVV E 
Sbjct: 306 IGAADASSIAIQWAMADIINNREILEKLREEIDSVVGKTRLVQETDLPNLPYLQAVVKEG 365

Query: 61  LRLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGS 120
           LRLHP  P+++RE  + C I G  V   T ++VN+YA+MRDP+SW +PD+F PERFL   
Sbjct: 366 LRLHPPTPLVVREFQEGCEIGGFFVPKNTTLIVNSYAMMRDPDSWQDPDEFKPERFLASL 425

Query: 121 SENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGDGGVD 180
           S        + K +   ++PFGSG+R CPGS+L  +    AI  MVQ FDW ++GD    
Sbjct: 426 S-----REEDKKEKILNFLPFGSGRRMCPGSNLGYIFVGTAIGMMVQCFDWEINGDKINM 485

Query: 181 LKPGSGFAAEMATPLVCYANPIHGT 206
            +   GF   MA PL C   P+  T
Sbjct: 486 EEATGGFLITMAHPLTCTPIPLPRT 505

[Close]

BLAST of Spg011178 vs. ExPASy Swiss-Prot
Match: Q9FH67 (Cytochrome P450 705A12 OS=Arabidopsis thaliana OX=3702 GN=CYP705A12 PE=3 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.3e-47
Identity = 94/197 (47.72%), Postives = 125/197 (63.45%), Query Frame = 0


Query: 1   MAGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNET 60
           + GTD++A  + W M E+LN P  ++KLR EI SVVG K+L+QESD P LPYL+AVV E 
Sbjct: 295 LGGTDSSAQTIQWTMAEILNNPGVLEKLRAEIDSVVGGKRLIQESDLPNLPYLQAVVKEG 354

Query: 61  LRLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGS 120
           LRLHPSAP+++R   + C +    V  KT ++VN YAV RDP+SW +PD F PERFL  S
Sbjct: 355 LRLHPSAPVLLRVFGESCEVKEFYVPEKTTLVVNLYAVNRDPDSWEDPDMFKPERFLVSS 414

Query: 121 SENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGDGGVD 180
              I     +++ Q  +Y+ FG G+R CP   LA +    AI AMVQ FDW + G+    
Sbjct: 415 ---ISGDEEKIREQAVKYVTFGGGRRTCPAVKLAHIFMETAIGAMVQCFDWRIKGEKVYM 474

Query: 181 LKPGSGFAAEMATPLVC 198
            +  SG + +MA PL C
Sbjct: 475 EEAVSGLSLKMAHPLKC 488

[Close]

BLAST of Spg011178 vs. ExPASy Swiss-Prot
Match: Q0JFI2 (Cytochrome P450 93G1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP93G1 PE=1 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.1e-46
Identity = 89/200 (44.50%), Postives = 128/200 (64.00%), Query Frame = 0


Query: 2   AGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNETL 61
           AG+DT+A  V W + EL+N PEA++K+R+EI +VVG  ++  E D P+LPYL+A   ETL
Sbjct: 312 AGSDTSAAMVEWMVAELMNHPEALRKVREEIEAVVGRDRIAGEGDLPRLPYLQAAYKETL 371

Query: 62  RLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGSS 121
           RL P+API  R+  ++  I G  V A+T V +N +A+ RDP  W EP +F PERFL G  
Sbjct: 372 RLRPAAPIAHRQSTEEIQIRGFRVPAQTAVFINVWAIGRDPAYWEEPLEFRPERFLAGG- 431

Query: 122 ENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGDGGVDL 181
              G   +E +GQ+F+++PFGSG+RGCPG  LAL   P  +AA++Q FDW    +  +D+
Sbjct: 432 ---GGEGVEPRGQHFQFMPFGSGRRGCPGMGLALQSVPAVVAALLQCFDWQCMDNKLIDM 491

Query: 182 KPGSGFAAEMATPLVCYANP 202
           +   G        L+ +A+P
Sbjct: 492 EEADGLVCARKHRLLLHAHP 507

[Close]

BLAST of Spg011178 vs. ExPASy TrEMBL
Match: A0A6J1J5T8 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111481610 PE=3 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.7e-98
Identity = 178/206 (86.41%), Postives = 192/206 (93.20%), Query Frame = 0


Query: 1   MAGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNET 60
           MAGTDTTATA+LWAM ELLNRP+++++LRKEIISVVG +KLVQESD P LPYLRAVVNET
Sbjct: 321 MAGTDTTATAILWAMAELLNRPKSMEELRKEIISVVGEEKLVQESDLPNLPYLRAVVNET 380

Query: 61  LRLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGS 120
           LRLHPSAP+IIRECLDDC INGSLVKAKTRVLVNAYAVMRDPESWSEPD+FLPERFLEGS
Sbjct: 381 LRLHPSAPMIIRECLDDCIINGSLVKAKTRVLVNAYAVMRDPESWSEPDRFLPERFLEGS 440

Query: 121 SENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGDGG-- 180
            ENIG+HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCA+AAMVQRFDW +DGDG   
Sbjct: 441 HENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAMAAMVQRFDWKVDGDGDGD 500

Query: 181 -VDLKPGSGFAAEMATPLVCYANPIH 204
            +DL PG GFAAEMATPLV YANPI+
Sbjct: 501 TIDLTPGPGFAAEMATPLVRYANPIN 526

[Close]

BLAST of Spg011178 vs. ExPASy TrEMBL
Match: A0A5D3DP65 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005640 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 4.9e-98
Identity = 178/204 (87.25%), Postives = 188/204 (92.16%), Query Frame = 0


Query: 1   MAGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNET 60
           MAGTDTTATA+LWAMGELLN PE+++KLRKEI SVVGNKK VQESD P LPYLRA+VNET
Sbjct: 318 MAGTDTTATAMLWAMGELLNCPESLQKLRKEITSVVGNKKPVQESDLPNLPYLRAIVNET 377

Query: 61  LRLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGS 120
           LRLHPSAPIIIRECLDDCNIN SL+KAKTRVL+NAYAVMRDPESWSEPDKFLPERFLEGS
Sbjct: 378 LRLHPSAPIIIRECLDDCNINDSLIKAKTRVLINAYAVMRDPESWSEPDKFLPERFLEGS 437

Query: 121 SENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGD--GG 180
            ENIG+HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFP AIA MVQRFDW + GD  G 
Sbjct: 438 HENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPRAIATMVQRFDWKIGGDNGGN 497

Query: 181 VDLKPGSGFAAEMATPLVCYANPI 203
           VDL  GSGFAAEMATPL+CYANPI
Sbjct: 498 VDLTLGSGFAAEMATPLICYANPI 521

[Close]

BLAST of Spg011178 vs. ExPASy TrEMBL
Match: A0A1S3B805 (LOW QUALITY PROTEIN: 3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103486788 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 4.9e-98
Identity = 178/204 (87.25%), Postives = 188/204 (92.16%), Query Frame = 0


Query: 1   MAGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNET 60
           MAGTDTTATA+LWAMGELLN PE+++KLRKEI SVVGNKK VQESD P LPYLRA+VNET
Sbjct: 331 MAGTDTTATAMLWAMGELLNCPESLQKLRKEITSVVGNKKPVQESDLPNLPYLRAIVNET 390

Query: 61  LRLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGS 120
           LRLHPSAPIIIRECLDDCNIN SL+KAKTRVL+NAYAVMRDPESWSEPDKFLPERFLEGS
Sbjct: 391 LRLHPSAPIIIRECLDDCNINDSLIKAKTRVLINAYAVMRDPESWSEPDKFLPERFLEGS 450

Query: 121 SENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGD--GG 180
            ENIG+HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFP AIA MVQRFDW + GD  G 
Sbjct: 451 HENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPRAIATMVQRFDWKIGGDNGGN 510

Query: 181 VDLKPGSGFAAEMATPLVCYANPI 203
           VDL  GSGFAAEMATPL+CYANPI
Sbjct: 511 VDLTLGSGFAAEMATPLICYANPI 534

[Close]

BLAST of Spg011178 vs. ExPASy TrEMBL
Match: A0A6J1F1K7 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111441548 PE=3 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 1.4e-97
Identity = 177/206 (85.92%), Postives = 192/206 (93.20%), Query Frame = 0


Query: 1   MAGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNET 60
           MAGTDTTATA+LWAM ELLNRP+++++LRKEIISVVG KKLVQESD P LPYLRAVVNET
Sbjct: 319 MAGTDTTATAILWAMAELLNRPKSMEELRKEIISVVGEKKLVQESDLPNLPYLRAVVNET 378

Query: 61  LRLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGS 120
           LRLHPSAP+IIRECLDDC ING+LVKAKTRVLVNAYAVMRDPESWSEPD+FLPERFLEGS
Sbjct: 379 LRLHPSAPLIIRECLDDCIINGTLVKAKTRVLVNAYAVMRDPESWSEPDRFLPERFLEGS 438

Query: 121 SENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGDGG-- 180
            ENIG+HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCA+AAMVQRFD+ +DGDG   
Sbjct: 439 HENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAMAAMVQRFDFKVDGDGDGD 498

Query: 181 -VDLKPGSGFAAEMATPLVCYANPIH 204
            +DL PG GFAAEMATPLV YANPI+
Sbjct: 499 TIDLTPGPGFAAEMATPLVRYANPIN 524

[Close]

BLAST of Spg011178 vs. ExPASy TrEMBL
Match: A0A2I4HPZ1 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Juglans regia OX=51240 GN=LOC109020255 PE=3 SV=2)

HSP 1 Score: 307.8 bits (787), Expect = 3.5e-80
Identity = 143/198 (72.22%), Postives = 166/198 (83.84%), Query Frame = 0


Query: 1   MAGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNET 60
           MAGTDT++ A+ WAMGELLN P+A KKLR+EI SVVG  KLV+ESD P LPYLRAV+ ET
Sbjct: 316 MAGTDTSSAAMQWAMGELLNNPKAFKKLREEINSVVGPNKLVKESDMPNLPYLRAVIKET 375

Query: 61  LRLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGS 120
           LRLHPSAP+IIREC +DC +NGS+VKAKTRVLVN YA+MRDP SW +PD+F+PERFLE S
Sbjct: 376 LRLHPSAPLIIRECAEDCQVNGSIVKAKTRVLVNVYAIMRDPVSWKDPDEFIPERFLESS 435

Query: 121 SENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGDGGVD 180
            E IG H+ME KGQNFRY+PFGSG+RGCPG+SLA+L+   AIAA+VQRFDW +     VD
Sbjct: 436 GERIGEHQMEFKGQNFRYLPFGSGRRGCPGASLAMLMMHPAIAALVQRFDWKLKDGEKVD 495

Query: 181 LKPGSGFAAEMATPLVCY 199
           L  G GFAAEMATPLVCY
Sbjct: 496 LTQGPGFAAEMATPLVCY 513

[Close]

BLAST of Spg011178 vs. TAIR 10
Match: AT2G42250.1 (cytochrome P450, family 712, subfamily A, polypeptide 1 )

HSP 1 Score: 287.0 bits (733), Expect = 1.2e-77
Identity = 132/202 (65.35%), Postives = 163/202 (80.69%), Query Frame = 0


Query: 1   MAGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNET 60
           MAGTDT+A A+ WAMG+L+N P+A  KLR+EI +VVG+K+LV+ESD P LPYLRAV+ ET
Sbjct: 309 MAGTDTSAAAMQWAMGQLINHPQAFNKLREEINNVVGSKRLVKESDVPNLPYLRAVLRET 368

Query: 61  LRLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGS 120
           LRLHPSAP+IIREC +DC +NG LVK+KTRVLVN YA+MRD E W++ D+F+PERFLE S
Sbjct: 369 LRLHPSAPLIIRECAEDCQVNGCLVKSKTRVLVNVYAIMRDSELWADADRFIPERFLESS 428

Query: 121 SENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGDGGVD 180
            E IG H+M+ KGQNFRY+PFGSG+RGCPG+SLA+ V    + ++VQRFDW       VD
Sbjct: 429 EEKIGEHQMQFKGQNFRYLPFGSGRRGCPGASLAMNVMHIGVGSLVQRFDWKSVDGQKVD 488

Query: 181 LKPGSGFAAEMATPLVCYANPI 203
           L  GSGF+AEMA PLVC  NP+
Sbjct: 489 LSQGSGFSAEMARPLVC--NPV 508

[Close]

BLAST of Spg011178 vs. TAIR 10
Match: AT3G20120.1 (cytochrome P450, family 705, subfamily A, polypeptide 21 )

HSP 1 Score: 198.4 bits (503), Expect = 5.8e-51
Identity = 97/200 (48.50%), Postives = 125/200 (62.50%), Query Frame = 0


Query: 2   AGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNETL 61
           AGTDT+A ++ W M E++N P  +K+LR+EI SVVG  +L+QE+D PKLPYL+AVV E L
Sbjct: 174 AGTDTSAQSIQWTMAEIINNPNILKRLREEIDSVVGKTRLIQETDLPKLPYLQAVVKEGL 233

Query: 62  RLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGSS 121
           RLHP  P+ +R   + C I G  V  KT ++ NAY +MRDP  W +P++F PERFL  S 
Sbjct: 234 RLHPPLPLFVRTFQEGCKIGGFYVPEKTTLIGNAYVMMRDPSVWEDPEEFKPERFLSSSR 293

Query: 122 ENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGDGGVDL 181
                 R E   Q  +YIPFGSG+RGCPGSSL  +    A+  MVQ FDW++ GD  V +
Sbjct: 294 STQEEERRE---QALKYIPFGSGRRGCPGSSLGYIFVGTAVGMMVQCFDWSIKGD-KVQM 353

Query: 182 KPGSGFAAEMATPLVCYANP 202
               G    MA  L C   P
Sbjct: 354 DEAGGLNLSMAHSLKCTPVP 369

[Close]

BLAST of Spg011178 vs. TAIR 10
Match: AT3G20120.2 (cytochrome P450, family 705, subfamily A, polypeptide 21 )

HSP 1 Score: 198.4 bits (503), Expect = 5.8e-51
Identity = 97/200 (48.50%), Postives = 125/200 (62.50%), Query Frame = 0


Query: 2   AGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNETL 61
           AGTDT+A ++ W M E++N P  +K+LR+EI SVVG  +L+QE+D PKLPYL+AVV E L
Sbjct: 174 AGTDTSAQSIQWTMAEIINNPNILKRLREEIDSVVGKTRLIQETDLPKLPYLQAVVKEGL 233

Query: 62  RLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGSS 121
           RLHP  P+ +R   + C I G  V  KT ++ NAY +MRDP  W +P++F PERFL  S 
Sbjct: 234 RLHPPLPLFVRTFQEGCKIGGFYVPEKTTLIGNAYVMMRDPSVWEDPEEFKPERFLSSSR 293

Query: 122 ENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGDGGVDL 181
                 R E   Q  +YIPFGSG+RGCPGSSL  +    A+  MVQ FDW++ GD  V +
Sbjct: 294 STQEEERRE---QALKYIPFGSGRRGCPGSSLGYIFVGTAVGMMVQCFDWSIKGD-KVQM 353

Query: 182 KPGSGFAAEMATPLVCYANP 202
               G    MA  L C   P
Sbjct: 354 DEAGGLNLSMAHSLKCTPVP 369

[Close]

BLAST of Spg011178 vs. TAIR 10
Match: AT2G14100.1 (cytochrome P450, family 705, subfamily A, polypeptide 13 )

HSP 1 Score: 196.1 bits (497), Expect = 2.9e-50
Identity = 94/197 (47.72%), Postives = 125/197 (63.45%), Query Frame = 0


Query: 1   MAGTDTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNET 60
           + GTDT+A  + W M +++ +P  +++LRKEI SVVG  +L+QE D P LPYL+AV+ E 
Sbjct: 311 LGGTDTSAQTIEWTMAKIIKKPNILERLRKEIDSVVGKTRLIQEKDLPNLPYLQAVIKEG 370

Query: 61  LRLHPSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGS 120
           LRLHP AP++ R+  D C I G  V   T ++VNAYAVMRDP+SW +PD+F PERFL  S
Sbjct: 371 LRLHPPAPLLGRKVTDGCTIGGCYVPKNTTLVVNAYAVMRDPDSWEDPDEFKPERFLASS 430

Query: 121 SENIGNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGDGGVD 180
                  + E + Q  +YIPFGSG+RGCPG +L  +    AI  MV  FDW  +GD    
Sbjct: 431 -----RGKEEEREQELKYIPFGSGRRGCPGVNLGYIFVGTAIGMMVHCFDWRTNGDKVNM 490

Query: 181 LKPGSGFAAEMATPLVC 198
            +  +G    MA PL C
Sbjct: 491 EETVAGITLNMAHPLRC 502

[Close]

BLAST of Spg011178 vs. TAIR 10
Match: AT1G28430.1 (cytochrome P450, family 705, subfamily A, polypeptide 24 )

HSP 1 Score: 193.4 bits (490), Expect = 1.9e-49
Identity = 95/199 (47.74%), Postives = 131/199 (65.83%), Query Frame = 0


Query: 5   DTTATAVLWAMGELLNRPEAIKKLRKEIISVVGNKKLVQESDFPKLPYLRAVVNETLRLH 64
           +++++   WAMGE+ N P   +KLR EI SVVG  +L+QESD PKLPYL+AVV E+LRLH
Sbjct: 312 ESSSSTTRWAMGEIFNNPRIFEKLRTEIDSVVGTTRLIQESDLPKLPYLQAVVKESLRLH 371

Query: 65  PSAPIIIRECLDDCNINGSLVKAKTRVLVNAYAVMRDPESWSEPDKFLPERFLEGSSENI 124
           P   ++ RE   DCNI G  +   T ++VNAYAVMRDP+ W +P++F PERFL+ S   +
Sbjct: 372 PVGAVLPREFTQDCNIGGFYIHEGTSLVVNAYAVMRDPDIWEDPNEFKPERFLDAS--RL 431

Query: 125 GNHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWNMDGDGGVDLKPG 184
           G    E K +  +++PFG+G+RGCPG  L   +    I  MVQ FDW ++GD  V+++ G
Sbjct: 432 GQEE-EKKEKTLKFLPFGAGRRGCPGLYLGYTLVETTIGVMVQCFDWEIEGD-KVNMQEG 491

Query: 185 SG--FAAEMATPLVCYANP 202
           SG  F  ++A PL C   P
Sbjct: 492 SGLRFFLDLAHPLKCTPIP 506

The following BLAST results are available for this feature:

BLAST of Spg011178 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905529.1	3.7e-100	84.62	3,9-dihydroxypterocarpan 6A-monooxygenase [Benincasa hispida]	[more]
XP_022982904.1	3.5e-98	86.41	3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita maxima]	[more]
KAG7017572.1	3.5e-98	86.41	3,9-dihydroxypterocarpan 6A-monooxygenase, partial [Cucurbita argyrosperma subsp...	[more]
XP_023528514.1	5.9e-98	85.92	3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita pepo subsp. pepo]	[more]
KAA0043713.1	1.0e-97	87.25	3,9-dihydroxypterocarpan 6A-monooxygenase [Cucumis melo var. makuwa] >TYK25421.1...	[more]

BLAST of Spg011178 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42798	8.1e-50	44.55	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1...	[more]
Q42799	4.0e-49	45.05	Cytochrome P450 93A2 OS=Glycine max OX=3847 GN=CYP93A2 PE=2 SV=1	[more]
Q0WQ07	9.9e-48	45.37	Cytochrome P450 705A1 OS=Arabidopsis thaliana OX=3702 GN=CYP705A1 PE=2 SV=1	[more]
Q9FH67	1.3e-47	47.72	Cytochrome P450 705A12 OS=Arabidopsis thaliana OX=3702 GN=CYP705A12 PE=3 SV=1	[more]
Q0JFI2	1.1e-46	44.50	Cytochrome P450 93G1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP93G1 PE=1 SV...	[more]

BLAST of Spg011178 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J5T8	1.7e-98	86.41	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A5D3DP65	4.9e-98	87.25	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3B805	4.9e-98	87.25	LOW QUALITY PROTEIN: 3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucumis melo O...	[more]
A0A6J1F1K7	1.4e-97	85.92	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A2I4HPZ1	3.5e-80	72.22	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Juglans regia OX=51240 GN=LOC109020...	[more]

BLAST of Spg011178 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42250.1	1.2e-77	65.35	cytochrome P450, family 712, subfamily A, polypeptide 1	[more]
AT3G20120.1	5.8e-51	48.50	cytochrome P450, family 705, subfamily A, polypeptide 21	[more]
AT3G20120.2	5.8e-51	48.50	cytochrome P450, family 705, subfamily A, polypeptide 21	[more]
AT2G14100.1	2.9e-50	47.72	cytochrome P450, family 705, subfamily A, polypeptide 13	[more]
AT1G28430.1	1.9e-49	47.74	cytochrome P450, family 705, subfamily A, polypeptide 24	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 55..66 score: 47.67 coord: 139..148 score: 59.07 coord: 2..19 score: 39.55 coord: 148..159 score: 34.16
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 1..181 e-value: 8.1E-58 score: 196.3
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 138..148 score: 55.79 coord: 54..72 score: 33.54 coord: 94..118 score: 40.03 coord: 11..37 score: 33.58 coord: 148..171 score: 28.13
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..192 e-value: 1.5E-64 score: 220.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 1..199
None	No IPR available	PANTHER	PTHR24298:SF204	CYTOCHROME P450, FAMILY 712, SUBFAMILY A, POLYPEPTIDE 1	coord: 1..200
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 1..200
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 141..150

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg011178.1	Spg011178.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen