Spg011113 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg011113
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionReverse transcriptase
Locationscaffold4: 25043946 .. 25044876 (+)
RNA-Seq ExpressionSpg011113
SyntenySpg011113
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTGAGATATTGAGCCCGAGGCTATATGGTACCGTGTGCACACAGGTTATATTCCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTGGGCTGGCCCCACTACGACAAAGACGATGGGAGTGCTGGGCGGGCCCCACTACATCGTAGGACTAGTAAACGTTGGTTGTACTGGGCGTGCCCTACACAACGTAGATCGGTCATGTTAGTTAAAACGTTTGATGTACTTAATATGCCTTGCTAGATTTTAATGACGAACTTGCGAGATATTTTAGAAAGCCTTTTTCATTACACGTTTATGTTAAACGCTTGAATTGAGCATATCTGTACTTTTAAAGGTTTATCACGTGTTTTGACTTCCTGATTTAGAGTTTTAAAGTTAGTCACTCACTGGGCTTCGTAGCTCATTCTTTCAAAATGTTTTTCCACTTTCAGGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCCAGAACAAGCTCCAGTACGTGGGTGGAGTTGTATATTGAGTCTGTTCATATTGTAAATGTTTTGTAAGGATTGGATAATATAGTCCTAGAGGTCGTGGTTGTATAAACCCTAGTTGTATATGTTTTTGGTTGTGACATCTATATCAGGTTATTATCCGAACCAAGTTATGTCAGGGCGGCACCTCGTGTTTGTTGGAAGTTTTTTTTTAGGGATTCCGCTGTGTTCTGTTTCATGTAGAATGTTTTATATCTATGATTGGTATGTTTATCAGGTTCAACGTTCAGGAGGGTCAGCGGGTATCGTTAGAGGAGGACGATGTCCGTTGGCTTCACGCCATCTTTCGGGCTAAGCTAGCAGGTGGTCCGGGAGGGGGTGTGACAATTCAAGTCCCCGGCAACAGCGAAAAAACTTGTTGGGCTCCTAGA

mRNA sequence

ATGACTGAGATATTGAGCCCGAGGCTATATGGTACCGTGTGCACACAGGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCCAGAACAAGCTCCAGTACGTGGGTGGAGTTGTATATTGAGTCTGTTCATATTGTAAATGTTTTGTTCAACGTTCAGGAGGGTCAGCGGGTATCGTTAGAGGAGGACGATGTCCGTTGGCTTCACGCCATCTTTCGGGCTAAGCTAGCAGGTGGTCCGGGAGGGGGTGTGACAATTCAAGTCCCCGGCAACAGCGAAAAAACTTGTTGGGCTCCTAGA

Coding sequence (CDS)

ATGACTGAGATATTGAGCCCGAGGCTATATGGTACCGTGTGCACACAGGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCCAGAACAAGCTCCAGTACGTGGGTGGAGTTGTATATTGAGTCTGTTCATATTGTAAATGTTTTGTTCAACGTTCAGGAGGGTCAGCGGGTATCGTTAGAGGAGGACGATGTCCGTTGGCTTCACGCCATCTTTCGGGCTAAGCTAGCAGGTGGTCCGGGAGGGGGTGTGACAATTCAAGTCCCCGGCAACAGCGAAAAAACTTGTTGGGCTCCTAGA

Protein sequence

MTEILSPRLYGTVCTQVEIELPVPDTLPTSAESSRTSSSTWVELYIESVHIVNVLFNVQEGQRVSLEEDDVRWLHAIFRAKLAGGPGGGVTIQVPGNSEKTCWAPR
Homology
BLAST of Spg011113 vs. NCBI nr
Match: TYK27998.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 115.9 bits (289), Expect = 2.1e-22
Identity = 64/107 (59.81%), Postives = 71/107 (66.36%), Query Frame = 0

Query: 1   MTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSRTSSSTWVE 60
           MTEILSPRLYGTVCTQ                 VEIELPVPDTLPTSAESS ++SSTW+E
Sbjct: 1   MTEILSPRLYGTVCTQVIFPLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLE 60

Query: 61  LYIESVHIVNVLFNVQEGQRVSLEEDDVRWLHAIFRAKLAGGPGGGV 91
           LY+E VH+              + EDDV WLHA+FRAK AGGPGGGV
Sbjct: 61  LYVEFVHVEMFWL------AGIVREDDVCWLHAVFRAKTAGGPGGGV 101

BLAST of Spg011113 vs. NCBI nr
Match: KAA0066851.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 115.9 bits (289), Expect = 2.1e-22
Identity = 64/107 (59.81%), Postives = 71/107 (66.36%), Query Frame = 0

Query: 1   MTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSRTSSSTWVE 60
           MTEILSPRLYGTVCTQ                 VEIELPVPDTLPTSAESS ++SSTW+E
Sbjct: 1   MTEILSPRLYGTVCTQVIFPLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLE 60

Query: 61  LYIESVHIVNVLFNVQEGQRVSLEEDDVRWLHAIFRAKLAGGPGGGV 91
           LY+E VH+              + EDDV WLHA+FRAK AGGPGGGV
Sbjct: 61  LYVEFVHVEMFWL------AGIVREDDVCWLHAVFRAKTAGGPGGGV 101

BLAST of Spg011113 vs. NCBI nr
Match: KAA0062407.1 (uncharacterized protein E6C27_scaffold154G002090 [Cucumis melo var. makuwa] >TYK26574.1 uncharacterized protein E5676_scaffold313G001830 [Cucumis melo var. makuwa])

HSP 1 Score: 111.7 bits (278), Expect = 3.9e-21
Identity = 59/104 (56.73%), Postives = 72/104 (69.23%), Query Frame = 0

Query: 1   MTEILSPRLYGTVCTQVEIELPVPDTLPTSAESSRTSSSTWVELYIESVHI--------- 60
           MTEILS RLYGTVCTQV+IELP+PDTLPTSAESS ++ +TW+ELY ESVH+         
Sbjct: 1   MTEILSLRLYGTVCTQVKIELPMPDTLPTSAESSESNFNTWLELYFESVHVEIFCYFTNQ 60

Query: 61  ----VNVLFNVQEGQRVSLEEDDVRWLHAIFRAKLAGGPGGGVT 92
               ++++FNV  G    +  DDV WLHA+FR K A  PG  VT
Sbjct: 61  VMSGLHLVFNVSVGSTGIVRGDDVCWLHAVFRDKTAVDPGECVT 104

BLAST of Spg011113 vs. NCBI nr
Match: TYK08952.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 106.7 bits (265), Expect = 1.3e-19
Identity = 62/118 (52.54%), Postives = 71/118 (60.17%), Query Frame = 0

Query: 1    MTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSRTSSSTWVE 60
            MTEILS RL GTVCTQ                 VEIELPVPDTLPTSAE   ++SSTW+E
Sbjct: 1286 MTEILSLRLDGTVCTQVIFPLSTLSVLRDNNAVVEIELPVPDTLPTSAEIFESNSSTWLE 1345

Query: 61   LYIESVHIVNVLFNVQ----------EGQRVSLEEDDVRWLHAIFRAKLAGGPGGGVT 92
            LY+ESVH+  + +             EG    +  DDV WLHA+FRAK AGGP GGVT
Sbjct: 1346 LYLESVHVEMLFYKPSDVRAASYVCWEGSAGIVRGDDVCWLHAVFRAKTAGGPEGGVT 1403

BLAST of Spg011113 vs. NCBI nr
Match: KAA0062409.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 100.1 bits (248), Expect = 1.2e-17
Identity = 51/88 (57.95%), Postives = 62/88 (70.45%), Query Frame = 0

Query: 17  VEIELPVPDTLPTSAESSRTSSSTWVELYIESVHI-------------VNVLFNVQEGQR 76
           VEIELPVPDTLPTSAESS ++SSTW+ELY ESVH+             ++++F+V  G  
Sbjct: 94  VEIELPVPDTLPTSAESSESNSSTWLELYFESVHVEMFCYFTNQVMSRLHLVFSVLVGST 153

Query: 77  VSLEEDDVRWLHAIFRAKLAGGPGGGVT 92
             +  DDV WLHA+FRAK  GGPGGGVT
Sbjct: 154 GIVRGDDVCWLHAVFRAKTVGGPGGGVT 181

BLAST of Spg011113 vs. ExPASy TrEMBL
Match: A0A5D3DX39 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G002140 PE=4 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.0e-22
Identity = 64/107 (59.81%), Postives = 71/107 (66.36%), Query Frame = 0

Query: 1   MTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSRTSSSTWVE 60
           MTEILSPRLYGTVCTQ                 VEIELPVPDTLPTSAESS ++SSTW+E
Sbjct: 1   MTEILSPRLYGTVCTQVIFPLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLE 60

Query: 61  LYIESVHIVNVLFNVQEGQRVSLEEDDVRWLHAIFRAKLAGGPGGGV 91
           LY+E VH+              + EDDV WLHA+FRAK AGGPGGGV
Sbjct: 61  LYVEFVHVEMFWL------AGIVREDDVCWLHAVFRAKTAGGPGGGV 101

BLAST of Spg011113 vs. ExPASy TrEMBL
Match: A0A5A7VMG9 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold271G002220 PE=4 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.0e-22
Identity = 64/107 (59.81%), Postives = 71/107 (66.36%), Query Frame = 0

Query: 1   MTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSRTSSSTWVE 60
           MTEILSPRLYGTVCTQ                 VEIELPVPDTLPTSAESS ++SSTW+E
Sbjct: 1   MTEILSPRLYGTVCTQVIFPLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLE 60

Query: 61  LYIESVHIVNVLFNVQEGQRVSLEEDDVRWLHAIFRAKLAGGPGGGV 91
           LY+E VH+              + EDDV WLHA+FRAK AGGPGGGV
Sbjct: 61  LYVEFVHVEMFWL------AGIVREDDVCWLHAVFRAKTAGGPGGGV 101

BLAST of Spg011113 vs. ExPASy TrEMBL
Match: A0A5A7V638 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G001830 PE=4 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.9e-21
Identity = 59/104 (56.73%), Postives = 72/104 (69.23%), Query Frame = 0

Query: 1   MTEILSPRLYGTVCTQVEIELPVPDTLPTSAESSRTSSSTWVELYIESVHI--------- 60
           MTEILS RLYGTVCTQV+IELP+PDTLPTSAESS ++ +TW+ELY ESVH+         
Sbjct: 1   MTEILSLRLYGTVCTQVKIELPMPDTLPTSAESSESNFNTWLELYFESVHVEIFCYFTNQ 60

Query: 61  ----VNVLFNVQEGQRVSLEEDDVRWLHAIFRAKLAGGPGGGVT 92
               ++++FNV  G    +  DDV WLHA+FR K A  PG  VT
Sbjct: 61  VMSGLHLVFNVSVGSTGIVRGDDVCWLHAVFRDKTAVDPGECVT 104

BLAST of Spg011113 vs. ExPASy TrEMBL
Match: A0A5D3CCC7 (DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold314G00430 PE=4 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 6.1e-20
Identity = 62/118 (52.54%), Postives = 71/118 (60.17%), Query Frame = 0

Query: 1    MTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSRTSSSTWVE 60
            MTEILS RL GTVCTQ                 VEIELPVPDTLPTSAE   ++SSTW+E
Sbjct: 1286 MTEILSLRLDGTVCTQVIFPLSTLSVLRDNNAVVEIELPVPDTLPTSAEIFESNSSTWLE 1345

Query: 61   LYIESVHIVNVLFNVQ----------EGQRVSLEEDDVRWLHAIFRAKLAGGPGGGVT 92
            LY+ESVH+  + +             EG    +  DDV WLHA+FRAK AGGP GGVT
Sbjct: 1346 LYLESVHVEMLFYKPSDVRAASYVCWEGSAGIVRGDDVCWLHAVFRAKTAGGPEGGVT 1403

BLAST of Spg011113 vs. ExPASy TrEMBL
Match: A0A5A7V9N3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold154G002110 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 5.7e-18
Identity = 51/88 (57.95%), Postives = 62/88 (70.45%), Query Frame = 0

Query: 17  VEIELPVPDTLPTSAESSRTSSSTWVELYIESVHI-------------VNVLFNVQEGQR 76
           VEIELPVPDTLPTSAESS ++SSTW+ELY ESVH+             ++++F+V  G  
Sbjct: 94  VEIELPVPDTLPTSAESSESNSSTWLELYFESVHVEMFCYFTNQVMSRLHLVFSVLVGST 153

Query: 77  VSLEEDDVRWLHAIFRAKLAGGPGGGVT 92
             +  DDV WLHA+FRAK  GGPGGGVT
Sbjct: 154 GIVRGDDVCWLHAVFRAKTVGGPGGGVT 181

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK27998.12.1e-2259.81reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0066851.12.1e-2259.81reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0062407.13.9e-2156.73uncharacterized protein E6C27_scaffold154G002090 [Cucumis melo var. makuwa] >TYK... [more]
TYK08952.11.3e-1952.54DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa][more]
KAA0062409.11.2e-1757.95reverse transcriptase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3DX391.0e-2259.81Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold38... [more]
A0A5A7VMG91.0e-2259.81Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27... [more]
A0A5A7V6381.9e-2156.73Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5D3CCC76.1e-2052.54DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A5A7V9N35.7e-1857.95Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold15... [more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg011113.1Spg011113.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090304 nucleic acid metabolic process
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0016740 transferase activity