Spg008499 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg008499 vs. NCBI nr
Match: XP_022146379.1 (ubiquitin carboxyl-terminal hydrolase 12-like [Momordica charantia])

HSP 1 Score: 92.0 bits (227), Expect = 5.8e-15
Identity = 83/257 (32.30%), Postives = 110/257 (42.80%), Query Frame = 0

Query: 1   LRSSRVEKPLHYTLKIQSFSLLKAALAGSKRDRYESQIFNAGG----------------- 60
           +RS+R EKPLHYTL+IQSFSLLK AL  S  DRYES+IFNAGG                 
Sbjct: 24  IRSTRDEKPLHYTLQIQSFSLLKTALVRSDCDRYESRIFNAGGYKWKLAMYPTGDVKRNG 83

Query: 61  ---------------------------------KEKC---------------------RI 120
                                            K+K                      ++
Sbjct: 84  SHDHISLYLVKAEDDIPTSEVNVVFTFLVYDSLKDKYLAVQDGIVRRFHAIKTEWGFEKL 143

Query: 121 ISL---------------CI--------------WRIRVRP------ESDYLSLILVFDG 148
           ISL               C               W++ + P      E+ +LSL L+ D 
Sbjct: 144 ISLDTFNDSSNGFLVDDCCAFGVDVVVMKLEGYRWKLWLYPKGCSNAETGFLSLYLILDS 203

BLAST of Spg008499 vs. NCBI nr
Match: XP_038874970.1 (uncharacterized protein LOC120067490 [Benincasa hispida])

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-13
Identity = 61/155 (39.35%), Postives = 85/155 (54.84%), Query Frame = 0

Query: 8   KPLHYTLKIQSFSLLKAALAGSKRDRYESQIFNAGGKEKCRIISLCIWRIRVRPESD--- 67
           K   YT KI +FS L        ++ YES  F          +    W+I + P  D   
Sbjct: 193 KNYKYTWKINNFSQL-------SKNVYESDPFT---------VDNYTWKIELYPRGDSLA 252

Query: 68  ---YLSLILVFDGFKEVSQGFQ--VEFELAVLSQLGDEDRRFSTKWWFDHKSKDKG---Y 127
              Y+S+ L FD F+++ QG +  VE+++AVLSQLG     +  K WFD ++K  G   +
Sbjct: 253 MTGYMSMFLRFDSFEKLPQGSRVYVEYDIAVLSQLGGAYTNYPVKHWFDGETKSWGFGNF 312

Query: 128 LSLSNLNE--SGFLHDDTMMVESKINVISTLKNCF 150
           +SLSN+ E   GFL DDT+MVE KINVIS LK+ +
Sbjct: 313 MSLSNVKEPMKGFLCDDTLMVEVKINVISILKSFY 331

BLAST of Spg008499 vs. NCBI nr
Match: XP_022146380.1 (probable inactive serine/threonine-protein kinase fnkC [Momordica charantia])

HSP 1 Score: 81.3 bits (199), Expect = 1.0e-11
Identity = 67/177 (37.85%), Postives = 84/177 (47.46%), Query Frame = 0

Query: 1   LRSSRVEKPLHYTLKIQSFSLLKAALAGSKRDRYESQIFNAGGKEKCRIISLCIWRIRVR 60
           +RS+R EKPLHYTL+IQSFSLLKAALA S RDRYESQIFNAGG +         W++ V 
Sbjct: 9   IRSTRDEKPLHYTLQIQSFSLLKAALASSNRDRYESQIFNAGGYK---------WKLAVY 68

Query: 61  PE-------SDYLSLILV--FDGFKEVSQGFQVEFELAVLSQLGDEDRRFSTKWWFDHKS 120
           P        +D++SL LV   D     S G    F                 +W F+   
Sbjct: 69  PNGDVKRNGADHISLYLVKADDDINPCSHGKVRRF------------HAMKAEWGFEK-- 128

Query: 121 KDKGYLSLSNLNES--GFLHDD---------TMMVESKINVISTLKNCFVHKSIPKT 158
                +SL   N+S  GF+  D          M  + K  V+S +K    HK   KT
Sbjct: 129 ----LISLDTFNDSSNGFVVGDCCGFGVDVVVMKCDGKGEVVSFIKEPKSHKYTWKT 158

BLAST of Spg008499 vs. NCBI nr
Match: XP_022146381.1 (ubiquitin carboxyl-terminal hydrolase 13-like [Momordica charantia])

HSP 1 Score: 78.2 bits (191), Expect = 8.7e-11
Identity = 74/217 (34.10%), Postives = 96/217 (44.24%), Query Frame = 0

Query: 1   LRSSRVEKPLHYTLKIQSFSLLKAALAGSKRDRYESQIFNAGGKEKCRIISLCIWRIRVR 60
           L  +R EKPLHYTL+IQSFSLLK ALA S  DRYESQIFNAGG +         W++ V 
Sbjct: 18  LSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGYK---------WKLAVY 77

Query: 61  PE-------SDYLSLILVFDGFKEVSQGFQVEFELAVLSQLGD--------EDRRF---S 120
           P        SD++SL LV       +    V F   V   L D        + RRF    
Sbjct: 78  PNGDVKRNGSDHISLYLVKAEDGIPTSEVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIQ 137

Query: 121 TKWWFDHKSKDKGYLSLSNLNES--GFLHDD---------TMMVESKINVISTLKNCFVH 180
           T+W F+        +SL   N+S  GFL  D             + K  V+S +K     
Sbjct: 138 TEWEFEK------LISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKE---- 197

Query: 181 KSIPKTNESILANLWSIINLDRVRKSRHSLEVSDQDG 189
              PK NE      W   N  ++ K+ +  +    +G
Sbjct: 198 ---PKNNE----YTWKTNNFSQLDKNMYESDPFTVEG 208

BLAST of Spg008499 vs. NCBI nr
Match: XP_022146383.1 (uncharacterized protein LOC111015611 [Momordica charantia])

HSP 1 Score: 73.6 bits (179), Expect = 2.1e-09
Identity = 53/128 (41.41%), Postives = 66/128 (51.56%), Query Frame = 0

Query: 1   LRSSRVEKPLHYTLKIQSFSLLKAALAGSKRDRYESQIFNAGGKEKCRIISLCIWRIRVR 60
           LRS R EKPLHYTLKI+SFSLLK ALA SKRDR+ SQIF+A G +         W++ + 
Sbjct: 34  LRSIRDEKPLHYTLKIKSFSLLKKALASSKRDRFVSQIFHASGYK---------WKLALY 93

Query: 61  PESD-------YLSLILVFDGFKEVSQGFQVEFELAVL-------SQLGDED------RR 109
           P  D       Y+SL LV    + VS   +V   L  L         L  +D       R
Sbjct: 94  PNGDKNRNGSGYISLYLVTAEDEIVSATSEVNVVLTFLVYDSLRDKYLAIQDGKVKRFHR 152

BLAST of Spg008499 vs. ExPASy TrEMBL
Match: A0A6J1CXZ0 (ubiquitin carboxyl-terminal hydrolase 12-like OS=Momordica charantia OX=3673 GN=LOC111015607 PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 2.8e-15
Identity = 83/257 (32.30%), Postives = 110/257 (42.80%), Query Frame = 0

Query: 1   LRSSRVEKPLHYTLKIQSFSLLKAALAGSKRDRYESQIFNAGG----------------- 60
           +RS+R EKPLHYTL+IQSFSLLK AL  S  DRYES+IFNAGG                 
Sbjct: 24  IRSTRDEKPLHYTLQIQSFSLLKTALVRSDCDRYESRIFNAGGYKWKLAMYPTGDVKRNG 83

Query: 61  ---------------------------------KEKC---------------------RI 120
                                            K+K                      ++
Sbjct: 84  SHDHISLYLVKAEDDIPTSEVNVVFTFLVYDSLKDKYLAVQDGIVRRFHAIKTEWGFEKL 143

Query: 121 ISL---------------CI--------------WRIRVRP------ESDYLSLILVFDG 148
           ISL               C               W++ + P      E+ +LSL L+ D 
Sbjct: 144 ISLDTFNDSSNGFLVDDCCAFGVDVVVMKLEGYRWKLWLYPKGCSNAETGFLSLYLILDS 203

BLAST of Spg008499 vs. ExPASy TrEMBL
Match: A0A6J1CX47 (probable inactive serine/threonine-protein kinase fnkC OS=Momordica charantia OX=3673 GN=LOC111015608 PE=4 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 5.0e-12
Identity = 67/177 (37.85%), Postives = 84/177 (47.46%), Query Frame = 0

Query: 1   LRSSRVEKPLHYTLKIQSFSLLKAALAGSKRDRYESQIFNAGGKEKCRIISLCIWRIRVR 60
           +RS+R EKPLHYTL+IQSFSLLKAALA S RDRYESQIFNAGG +         W++ V 
Sbjct: 9   IRSTRDEKPLHYTLQIQSFSLLKAALASSNRDRYESQIFNAGGYK---------WKLAVY 68

Query: 61  PE-------SDYLSLILV--FDGFKEVSQGFQVEFELAVLSQLGDEDRRFSTKWWFDHKS 120
           P        +D++SL LV   D     S G    F                 +W F+   
Sbjct: 69  PNGDVKRNGADHISLYLVKADDDINPCSHGKVRRF------------HAMKAEWGFEK-- 128

Query: 121 KDKGYLSLSNLNES--GFLHDD---------TMMVESKINVISTLKNCFVHKSIPKT 158
                +SL   N+S  GF+  D          M  + K  V+S +K    HK   KT
Sbjct: 129 ----LISLDTFNDSSNGFVVGDCCGFGVDVVVMKCDGKGEVVSFIKEPKSHKYTWKT 158

BLAST of Spg008499 vs. ExPASy TrEMBL
Match: A0A6J1CZ69 (ubiquitin carboxyl-terminal hydrolase 13-like OS=Momordica charantia OX=3673 GN=LOC111015609 PE=4 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 4.2e-11
Identity = 74/217 (34.10%), Postives = 96/217 (44.24%), Query Frame = 0

Query: 1   LRSSRVEKPLHYTLKIQSFSLLKAALAGSKRDRYESQIFNAGGKEKCRIISLCIWRIRVR 60
           L  +R EKPLHYTL+IQSFSLLK ALA S  DRYESQIFNAGG +         W++ V 
Sbjct: 18  LSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGYK---------WKLAVY 77

Query: 61  PE-------SDYLSLILVFDGFKEVSQGFQVEFELAVLSQLGD--------EDRRF---S 120
           P        SD++SL LV       +    V F   V   L D        + RRF    
Sbjct: 78  PNGDVKRNGSDHISLYLVKAEDGIPTSEVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIQ 137

Query: 121 TKWWFDHKSKDKGYLSLSNLNES--GFLHDD---------TMMVESKINVISTLKNCFVH 180
           T+W F+        +SL   N+S  GFL  D             + K  V+S +K     
Sbjct: 138 TEWEFEK------LISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKE---- 197

Query: 181 KSIPKTNESILANLWSIINLDRVRKSRHSLEVSDQDG 189
              PK NE      W   N  ++ K+ +  +    +G
Sbjct: 198 ---PKNNE----YTWKTNNFSQLDKNMYESDPFTVEG 208

BLAST of Spg008499 vs. ExPASy TrEMBL
Match: A0A6J1CYF6 (uncharacterized protein LOC111015611 OS=Momordica charantia OX=3673 GN=LOC111015611 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.0e-09
Identity = 53/128 (41.41%), Postives = 66/128 (51.56%), Query Frame = 0

Query: 1   LRSSRVEKPLHYTLKIQSFSLLKAALAGSKRDRYESQIFNAGGKEKCRIISLCIWRIRVR 60
           LRS R EKPLHYTLKI+SFSLLK ALA SKRDR+ SQIF+A G +         W++ + 
Sbjct: 34  LRSIRDEKPLHYTLKIKSFSLLKKALASSKRDRFVSQIFHASGYK---------WKLALY 93

Query: 61  PESD-------YLSLILVFDGFKEVSQGFQVEFELAVL-------SQLGDED------RR 109
           P  D       Y+SL LV    + VS   +V   L  L         L  +D       R
Sbjct: 94  PNGDKNRNGSGYISLYLVTAEDEIVSATSEVNVVLTFLVYDSLRDKYLAIQDGKVKRFHR 152

BLAST of Spg008499 vs. ExPASy TrEMBL
Match: A0A6J1CZF7 (uncharacterized protein LOC111015610 OS=Momordica charantia OX=3673 GN=LOC111015610 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.4e-09
Identity = 67/187 (35.83%), Postives = 85/187 (45.45%), Query Frame = 0

Query: 1   LRSSRVEKPLHYTLKIQSFSLLKAALAGSKRDRYESQIFNAGGKEKCRIISLCIWRIRVR 60
           LRS R EKPLHYTL+IQ FSLLK ALA S RDRYES IF+  G +         W++ V 
Sbjct: 54  LRSIRDEKPLHYTLQIQPFSLLKTALATSSRDRYESPIFSVAGFK---------WKLAVY 113

Query: 61  PESD-------YLSLILVFDGFKEVSQGFQVEFELAVLSQLGD--------EDRRF---S 120
           P  D       ++SL LV       +    V F + V   L D        + RRF    
Sbjct: 114 PSGDVKRNGSNHISLYLVTAEDDIPTLDLTVVFTILVYDNLRDNYLAVLDGKVRRFHAMK 173

Query: 121 TKWWFDHKSKDKGYLSLSNLNES--GFLHDD---------TMMVESKINVISTLKNCFVH 159
            +W F+        +SL   N+S  GFL DD          M  + K   +S +K    +
Sbjct: 174 KEWGFEK------LVSLETFNDSSNGFLVDDCCAFGVDIFVMKCDGKGEALSLIKQPKNN 225

BLAST of Spg008499 vs. TAIR 10
Match: AT3G17380.1 (TRAF-like family protein )

HSP 1 Score: 46.2 bits (108), Expect = 3.4e-05
Identity = 46/153 (30.07%), Postives = 71/153 (46.41%), Query Frame = 0

Query: 9   PLHYTLKIQSFSLL-KAALAGSKRDRYESQIFNAGGKEKCRIISLCIWRIRVRPE----- 68
           P HY +KI+SFSLL K A+     +RYE++ F AGG +         W++ + P      
Sbjct: 19  PTHYMVKIESFSLLTKHAI-----ERYETESFEAGGYK---------WKLVLYPNGNKSK 78

Query: 69  --SDYLSLILVFDGFKEVSQGFQV--EFELAVLSQ-------LGDEDRRFST---KWWFD 128
              D++S+ L       +S G++V   F L +L Q       L   +RRF +   +W FD
Sbjct: 79  NTKDHVSVYLSLADSSSLSPGWEVYAVFRLYLLDQNKDNYLILQGNERRFHSVKREWGFD 138

Query: 129 HKSKDKGYLSLSNLNESGFLHDDTMMVESKINV 142
                  +   SN    G+L +DT M  + + V
Sbjct: 139 KFIPTGTFSDASN----GYLMEDTCMFGADVFV 153