Spg008436 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg008436
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCalmodulin-binding protein 60 G-like
Locationscaffold10: 33594594 .. 33598386 (-)
RNA-Seq ExpressionSpg008436
SyntenySpg008436
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCGAGAGACGCCACCAGCAAAAACAAGAAGAAAAGAGTCTCTTCTTCTGATGACCAAGTTCCAGAGGTTGAAGAAGGGGCAAAGAGAAGCAGCTTTCCACCTGGGTTTGTGTGTGTGTTTTTTTTTTTTTTTTTAAAAATTTTTTCCTTATCTCTCTCTTTTGATCTCTGCAGTCTGCATAACCGTTGTATGTTTGTTGCTGTATCTTTTCTCTTTCTTCCACTTGGTACTTTAGATTTTTTATTGTTTTTTTTTTCTTAATTTTTCCTATTCCAACACCCAATAGGGTTTAGTTTCTCTCTCTTCATTTCCTTTCATTTTTAATTTGCCCTATTTTTTTTTTCCCTATTTTATTTTTTGGGTTCTCTCCATTAATTCGTGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTCTAGTTCTGAAGCTGATTTTTCGTCGATCGTACTTGCCCATTTATTCTCCCAGCCGCAGCCTGATGAAATCCATCATTTGATTCAGCAAAGAGTGGTTGAACGTTTCTTTGTTGAACAATGGCCTCAAATGGAACCTTGGTTTCGCCAAGTGGTATTTTCATCATCTTTTTGATATTTTCTTTTATCGTTCTCATCTTTTCAATGTTTGTGCAAAAATTAAAACTAGGGAATAATCTAGAGAATTAATTGATCACATCTACGTCTATTGTTTTTTTAGATATGCGAGGTCACAAACAGCCTGATTAAGTCTCACGTTGACAACCTTGTGCTTGGTAAAAGGTCAGTGTTTTAATCAGCTGGAAATTATAAAACCCCAAATAACTTTTGTATTCCCTTGTTGTCTGCATTTGTGCAAATTAAGGATAATCTTGTAAACTGTTGTTGATGAGACATGTGTAAGTGTAGTGAGTGACAGTTAGAGAGAGAGAGTGAATGAGAGGGAAAACAAATTAAAGGGTGCGAGAGTGAGGAAGAAAAGAGGATCATATTTGGAGATCGAGCAGAAGGAATGGGGTTTACGATTCACGAAAAAGAGCTAGGAATGAAATCTGGAATGCTTGTGTGTATATGTAGGCTTTGTATTTGTATTTGTTAGGTAAATGACGATTGGGTTTCGTGTGCATATATGTATAAATTATATTAGATAAGTATGAAGTTTCATCTTAAAACTAATTGGCAAGGAGAGGAATAATCCATATATCTTATAAATTGTAGAGTCTCCACTTATCTTTTCAGTGTGAGATCCTCAACAGGAAATTTAGAGTTAGTCTATGTATAAGTGTAGTGAGGAAATTTAGAGTTAGTCTATGTATAAGTGTAGTGAGTGACAGTTAGAGAGAGAGTGTGAATGAAAGGGAAAACAAATTAAAGGGTGGGAGAGTGAGGAAGAAAAGAGGATCATATTTGAAGGAATGGGGTTTATGATTCACAAAAAAGAGGAATGAAATCTGGAATGCTTGTGTATATGTAGGGCTTTGTATTTGTATTTGCTAGGCAAATGACGGTTGGATTTTGTGTGCATATATGTATAAATTATATTAGATAAACATGGAGTTTCATCTCAAAACTAATTGGCAAGGAGATGAGTAATCCATATATCTTATAAATTGTGAAGTCTCCATTCATCTTTTTCATGATGTGAGATCCTTAACAGGGGAGAAGATGAGATTGGAAAATACATAAATACTAGAGCGGGAAGATCGGCCTGGAGATTACGGTTCAGCAGTCGAATTGCAGCGGAGATTTTCACAAATAACAAGATAAGGGATGAAAAGGGTGAGGCATTGAAGGTTGAATTAATTTGTGATGTTGGCAGCTCCATGGCGCCAAGTCCTTTATTGTTAAATGCCCAGATTGAGTTGTTCATCCTGGAGAATAATACAGAAAAGTTTGGTAGTAGCAACCAACGAGAAGATGAAATTTATTGGACTTTGAGTGATTTCAAGGCAAATATTTTATCTCCAAGGGATGGGAAACGACCTTTAATTATTGGTACTGATGTGAAACTTTATCTGGAAAATGGAGTTGGGTCTATGGAGAATCTGACTGTCACAGATAACTCAAGCTGGACCAAAACTAAATGCTTTCGCTTGGGAGCCAAAATCATAGACAACAACATTTTAGCTGACTTCCCAAGAATTCAAGAAGCCGTATCACAACCCTTTCAAGTCAAGGATCACCGAGGGGAAGGTATGTCTTTCTGTCCGGCCCGTCCTTTAGCATGTGTAGGTTGAAATTTAGGGGCTCCAAATTGAAAAAGTTGTTTAACAAAAAAAAATATTTTGGCGTGCATGCCCAAGGAAAAACAGAAATTGGACCAAAAATTTCAATTTCTCACCTCTTCAACCAAGAAAAACCGCTTTCAATTTCTCCTTTTGTCAATTTCCTTGTTAAATTAAATATCAAATCTTTTAATCTTCTCCCTTCTCATCTTAGAAATTCTATATTTTAGGGTATCATGCATTATTTTGAGAAATATGACAGGGTCTGTTCTCATTTGGGACAGACATGCTCTCTATAAATAAATAAGAAGCATAGAGATCTTTCTATATATCCCATTAAAATCTTGACAACAAAGAAGGAAAAAGGTAGAAAATGAGATTTTTTTTTTAATCTTTTGTCAACATTTTAACAACGGTATGTAAGGGTTCGCAGCTAAGCATTTTTCAATAAATAATACAACCTTCTTCTTTCCCTTTCTAATTTTCGTTTTTCTGTACTGATTATTCTTTGGTAATAATCTTAGTGAACAAAAAACATCACCCTCCAAGCTGGGAAGATCCCGTATGGAGACTCGAAGGCATAGGCAAAGGTGGCGTCTATCACACAAACTTATCTAACCAAGGCATCTATACTGTCGGTGACTTCTTGATCGCTCTCTGGCGAAACGCTAACCATCTCAGAATGGTACCTATAACACTACCTCCTTCCATGAGTTTCTTTGTATAGTTATCTACCTTCTTCCTATGCTTTTGTTTTAAAAAACCAAGCCAAGTTTTGAAAACCAAAATGATTGAGAATGATCGAGAGTTATCAAGTTTTATATTGTCATACATGCATAAGCAGTTACTCGGACAGAGAGTCTTAGACAGCACGTGGAACAAGATGATTGAGAATGCTCAGGAGTGTGTTGATGTTCCTAGTACTTATAATCAAAGCTCCAAGGTTACTCTCTTTTCTTTCTCTCTCCCTTTGGATGAGCTTAGTTAACTAAGTTGATCATATAATTATATGAAACAACAGACAGATTTCGTGGAAACTAATAATCAAGTCCTGCACGAGGAACCTTTCAACGATAATTTTCTAGCTGATGAGCTTCCATATTTCTGTGAAGATCAACCACGGATGTCCATGGTGAACTTTAATGGTACATAAATGGATTTTTTTCTACTACAAAACCTCTGATTTTGACGTTTGTCTAAACCTTATATAATTTTGAACGACAGATCTAGTGACACCAAATGGAGAAGCTTCAACCTCACATGAACCAGACTACACGAACACTCAATCTGGTAATACATATTATCAAGGAGTTGAACCAAGTTATTGCCCAAATCCACCTCATCCAAACATACGACCATAGTTCATACATCTCTACCGCAAGAGACATAAAAGTGAGAGACTTCTTCCTCAGAATCAATTCTCGCATTTATATATATATTATATATAAAGATAACTATAAAAACCATGTTTGATTGATGGTCTTTTAGAGCGACGTTTTCAATTAGTCTCACTTTGTTTGTTATATGGATGTTGTTTTGCATTATTATTAAACCTGTTTGATTGATGGTCTTTTTAGAACATGTTTTG

mRNA sequence

ATGGATTCGAGAGACGCCACCAGCAAAAACAAGAAGAAAAGAGTCTCTTCTTCTGATGACCAAGTTCCAGAGGTTGAAGAAGGGGCAAAGAGAAGCAGCTTTCCACCTGGGGGAGAAGATGAGATTGGAAAATACATAAATACTAGAGCGGGAAGATCGGCCTGGAGATTACGGTTCAGCAGTCGAATTGCAGCGGAGATTTTCACAAATAACAAGATAAGGGATGAAAAGGGTGAGGCATTGAAGGTTGAATTAATTTGTGATGTTGGCAGCTCCATGGCGCCAAGTCCTTTATTGTTAAATGCCCAGATTGAGTTGTTCATCCTGGAGAATAATACAGAAAAGTTTGGTAGTAGCAACCAACGAGAAGATGAAATTTATTGGACTTTGAGTGATTTCAAGGCAAATATTTTATCTCCAAGGGATGGGAAACGACCTTTAATTATTGGTACTGATGTGAAACTTTATCTGGAAAATGGAGTTGGGTCTATGGAGAATCTGACTGTCACAGATAACTCAAGCTGGACCAAAACTAAATGCTTTCGCTTGGGAGCCAAAATCATAGACAACAACATTTTAGCTGACTTCCCAAGAATTCAAGAAGCCGTATCACAACCCTTTCAAGTCAAGGATCACCGAGGGGAAGTGAACAAAAAACATCACCCTCCAAGCTGGGAAGATCCCGTATGGAGACTCGAAGGCATAGGCAAAGGTGGCGTCTATCACACAAACTTATCTAACCAAGGCATCTATACTGTCGGTGACTTCTTGATCGCTCTCTGGCGAAACGCTAACCATCTCAGAATGTTACTCGGACAGAGAGTCTTAGACAGCACGTGGAACAAGATGATTGAGAATGCTCAGGAGTGTGTTGATGTTCCTAGTACTTATAATCAAAGCTCCAAGACAGATTTCGTGGAAACTAATAATCAAGTCCTGCACGAGGAACCTTTCAACGATAATTTTCTAGCTGATGAGCTTCCATATTTCTGTGAAGATCAACCACGGATGTCCATGGTGAACTTTAATGATCTAGTGACACCAAATGGAGAAGCTTCAACCTCACATGAACCAGACTACACGAACACTCAATCTGGTAATACATATTATCAAGGAGTTGAACCAAGTTATTGCCCAAATCCACCTCATCCAAACATACGACCATAG

Coding sequence (CDS)

ATGGATTCGAGAGACGCCACCAGCAAAAACAAGAAGAAAAGAGTCTCTTCTTCTGATGACCAAGTTCCAGAGGTTGAAGAAGGGGCAAAGAGAAGCAGCTTTCCACCTGGGGGAGAAGATGAGATTGGAAAATACATAAATACTAGAGCGGGAAGATCGGCCTGGAGATTACGGTTCAGCAGTCGAATTGCAGCGGAGATTTTCACAAATAACAAGATAAGGGATGAAAAGGGTGAGGCATTGAAGGTTGAATTAATTTGTGATGTTGGCAGCTCCATGGCGCCAAGTCCTTTATTGTTAAATGCCCAGATTGAGTTGTTCATCCTGGAGAATAATACAGAAAAGTTTGGTAGTAGCAACCAACGAGAAGATGAAATTTATTGGACTTTGAGTGATTTCAAGGCAAATATTTTATCTCCAAGGGATGGGAAACGACCTTTAATTATTGGTACTGATGTGAAACTTTATCTGGAAAATGGAGTTGGGTCTATGGAGAATCTGACTGTCACAGATAACTCAAGCTGGACCAAAACTAAATGCTTTCGCTTGGGAGCCAAAATCATAGACAACAACATTTTAGCTGACTTCCCAAGAATTCAAGAAGCCGTATCACAACCCTTTCAAGTCAAGGATCACCGAGGGGAAGTGAACAAAAAACATCACCCTCCAAGCTGGGAAGATCCCGTATGGAGACTCGAAGGCATAGGCAAAGGTGGCGTCTATCACACAAACTTATCTAACCAAGGCATCTATACTGTCGGTGACTTCTTGATCGCTCTCTGGCGAAACGCTAACCATCTCAGAATGTTACTCGGACAGAGAGTCTTAGACAGCACGTGGAACAAGATGATTGAGAATGCTCAGGAGTGTGTTGATGTTCCTAGTACTTATAATCAAAGCTCCAAGACAGATTTCGTGGAAACTAATAATCAAGTCCTGCACGAGGAACCTTTCAACGATAATTTTCTAGCTGATGAGCTTCCATATTTCTGTGAAGATCAACCACGGATGTCCATGGTGAACTTTAATGATCTAGTGACACCAAATGGAGAAGCTTCAACCTCACATGAACCAGACTACACGAACACTCAATCTGGTAATACATATTATCAAGGAGTTGAACCAAGTTATTGCCCAAATCCACCTCATCCAAACATACGACCATAG

Protein sequence

MDSRDATSKNKKKRVSSSDDQVPEVEEGAKRSSFPPGGEDEIGKYINTRAGRSAWRLRFSSRIAAEIFTNNKIRDEKGEALKVELICDVGSSMAPSPLLLNAQIELFILENNTEKFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGSMENLTVTDNSSWTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPPSWEDPVWRLEGIGKGGVYHTNLSNQGIYTVGDFLIALWRNANHLRMLLGQRVLDSTWNKMIENAQECVDVPSTYNQSSKTDFVETNNQVLHEEPFNDNFLADELPYFCEDQPRMSMVNFNDLVTPNGEASTSHEPDYTNTQSGNTYYQGVEPSYCPNPPHPNIRP
Homology
BLAST of Spg008436 vs. NCBI nr
Match: XP_038879916.1 (calmodulin-binding protein 60 G-like isoform X1 [Benincasa hispida])

HSP 1 Score: 272.3 bits (695), Expect = 6.4e-69
Identity = 158/316 (50.00%), Postives = 208/316 (65.82%), Query Frame = 0

Query: 56  RLRFSSRIAAEIFTNNKIRDEKGEALKVELICDVGS-----SMAPSPLLLNAQIELFILE 115
           +L FS+ I+  IFTNN+I+ E GE LKV LI D  +     S  PS    +A +E FIL 
Sbjct: 125 KLCFSNSISPLIFTNNEIKSEHGEPLKV-LIHDATNSNTIISTGPSS---SALVEFFIL- 184

Query: 116 NNTEKFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGSMENLTVT 175
               +F SS +++DE  WT SDF  +IL+PRDGKRPLIIG+D +LYL+NGVG ++NL VT
Sbjct: 185 ---GEFDSSKRQDDETPWTSSDFDKSILTPRDGKRPLIIGSDRQLYLQNGVGFVKNLIVT 244

Query: 176 DNSSWTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPPSWEDPVW 235
           DNSSW K+K FRLGA+I D      F RI EAVS+PF+V D RGE N+KHHPP  ED +W
Sbjct: 245 DNSSWMKSKMFRLGARIKDEKSQVQFGRIGEAVSRPFRVMDQRGEGNQKHHPPRREDEIW 304

Query: 236 RLEGIGKGGVYHTNLSNQGIYTVGDFLIALWRNAN--HLRMLLGQRVLDSTWNKMIENAQ 295
           RLEGI K G+YH NLS+QGI TVGDFL A  +N N   LRM+LG+RVLD TWN M++NA+
Sbjct: 305 RLEGISKDGIYHKNLSSQGIKTVGDFLKAYQKNNNPTTLRMMLGKRVLDKTWNMMVKNAE 364

Query: 296 ECV--DVPSTYNQSSKTDFVETNNQVLHEEPFNDNFLADELPY-FCEDQPRMSMVN--FN 355
           EC+  +VP   +++ + + +   NQV+ +E F  NF   +  +    D PR+ +     +
Sbjct: 365 ECIVDNVPINDDRTVQANNLAEINQVMGDEAFQGNFGFMQNNFGESNDLPRLRINEGLLD 424

Query: 356 DLVTPNGEASTSHEPD 360
            +V    +AS  HE D
Sbjct: 425 SVVNYRAQASNIHEVD 432

BLAST of Spg008436 vs. NCBI nr
Match: XP_038879917.1 (calmodulin-binding protein 60 G-like isoform X2 [Benincasa hispida])

HSP 1 Score: 270.4 bits (690), Expect = 2.4e-68
Identity = 149/276 (53.99%), Postives = 192/276 (69.57%), Query Frame = 0

Query: 56  RLRFSSRIAAEIFTNNKIRDEKGEALKVELICDVGS-----SMAPSPLLLNAQIELFILE 115
           +L FS+ I+  IFTNN+I+ E GE LKV LI D  +     S  PS    +A +E FIL 
Sbjct: 125 KLCFSNSISPLIFTNNEIKSEHGEPLKV-LIHDATNSNTIISTGPSS---SALVEFFIL- 184

Query: 116 NNTEKFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGSMENLTVT 175
               +F SS +++DE  WT SDF  +IL+PRDGKRPLIIG+D +LYL+NGVG ++NL VT
Sbjct: 185 ---GEFDSSKRQDDETPWTSSDFDKSILTPRDGKRPLIIGSDRQLYLQNGVGFVKNLIVT 244

Query: 176 DNSSWTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPPSWEDPVW 235
           DNSSW K+K FRLGA+I D      F RI EAVS+PF+V D RGE N+KHHPP  ED +W
Sbjct: 245 DNSSWMKSKMFRLGARIKDEKSQVQFGRIGEAVSRPFRVMDQRGEGNQKHHPPRREDEIW 304

Query: 236 RLEGIGKGGVYHTNLSNQGIYTVGDFLIALWRNAN--HLRMLLGQRVLDSTWNKMIENAQ 295
           RLEGI K G+YH NLS+QGI TVGDFL A  +N N   LRM+LG+RVLD TWN M++NA+
Sbjct: 305 RLEGISKDGIYHKNLSSQGIKTVGDFLKAYQKNNNPTTLRMMLGKRVLDKTWNMMVKNAE 364

Query: 296 ECV--DVPSTYNQSSKTDFVETNNQVLHEEPFNDNF 323
           EC+  +VP   +++ + + +   NQV+ +E F  NF
Sbjct: 365 ECIVDNVPINDDRTVQANNLAEINQVMGDEAFQGNF 392

BLAST of Spg008436 vs. NCBI nr
Match: XP_008450592.1 (PREDICTED: calmodulin-binding protein 60 G-like [Cucumis melo] >XP_008450593.1 PREDICTED: calmodulin-binding protein 60 G-like [Cucumis melo] >KAA0050926.1 calmodulin-binding protein 60 G-like [Cucumis melo var. makuwa] >TYK10274.1 calmodulin-binding protein 60 G-like [Cucumis melo var. makuwa])

HSP 1 Score: 269.2 bits (687), Expect = 5.4e-68
Identity = 144/271 (53.14%), Postives = 189/271 (69.74%), Query Frame = 0

Query: 55  WRLRFSSRIAAEIFTNNKIRDEKGEALKVELICDV--GSSMAPSPLLLNAQIELFILENN 114
           ++L F +   + IFTNN+++ E GE LKV  ICD    +S+  +  L +AQ+EL IL   
Sbjct: 127 FKLCFLNSFPSVIFTNNEVKSEDGEPLKV-AICDATNSNSIVSTGPLSSAQVELCILPG- 186

Query: 115 TEKFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGSMENLTVTDN 174
             +F SSN+R++E  WT S F  +IL+PRDGKRPLIIG D ++YL++GV  + NL +TDN
Sbjct: 187 --EFDSSNRRDEETPWTSSYFNKSILTPRDGKRPLIIGDDRQVYLKDGVRLINNLIITDN 246

Query: 175 SSWTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPPSWEDPVWRL 234
           SSW K+K FRLG KI D  +LA+F RI EA+S+PF+V D RGEVN+KHHPP WED VWRL
Sbjct: 247 SSWMKSKKFRLGVKIKDERVLAEFVRIGEAISEPFRVMDQRGEVNQKHHPPRWEDEVWRL 306

Query: 235 EGIGKGGVYHTNLSNQGIYTVGDFLIALWRN-ANHLRMLLGQRVLDSTWNKMIENAQECV 294
           EGI K G+YH NLS++GI TVGDFL A  +N  + LRM+LG+RVLD TW  M++NA ECV
Sbjct: 307 EGIAKDGIYHKNLSSKGIRTVGDFLKAYHQNDPDTLRMMLGKRVLDKTWKMMVQNACECV 366

Query: 295 DVPSTYNQSSKTDFVETNNQVLHEEPFNDNF 323
           +     NQ  + +     NQVL +E F + F
Sbjct: 367 EDTFDNNQVFQPNSFLEFNQVL-DETFEETF 392

BLAST of Spg008436 vs. NCBI nr
Match: XP_031743132.1 (calmodulin-binding protein 60 G isoform X1 [Cucumis sativus])

HSP 1 Score: 267.7 bits (683), Expect = 1.6e-67
Identity = 150/275 (54.55%), Postives = 186/275 (67.64%), Query Frame = 0

Query: 56  RLRFSSRIAAEIFTNNKIRDEKGEALKVELICDV---GSSMAPSPLLLNAQIELFILENN 115
           RL F +   + IFTNN+I+ E GE LKV  ICD     S ++ SP L + Q+EL IL   
Sbjct: 129 RLFFLNSFPSVIFTNNEIKSEDGEPLKV-AICDTTNCNSIVSTSP-LSSVQVELCILPG- 188

Query: 116 TEKFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGSMENLTVTDN 175
             +F SSN+R++E  WT S F  +IL+PRDGKRPLIIG D ++YL++GVG + NL +TDN
Sbjct: 189 --EFDSSNRRDEETPWTSSYFNTSILTPRDGKRPLIIGNDRQVYLKDGVGFINNLIITDN 248

Query: 176 SSWTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPPSWEDPVWRL 235
           SSW K+K FRLGAKI D  I A F RI EAVSQPF+V D RGEVN+KHHPP  +D VWRL
Sbjct: 249 SSWMKSKKFRLGAKITDERIPAGFGRIGEAVSQPFRVMDQRGEVNQKHHPPRMDDEVWRL 308

Query: 236 EGIGKGGVYHTNLSNQGIYTVGDFLIALWR--NANHLRMLLGQRVLDSTWNKMIENAQEC 295
           EGI K G+YH NLS+QGI TVGDFL A  +  N N LR +LG+RVLD TW  M++NA+EC
Sbjct: 309 EGIAKYGIYHKNLSSQGIKTVGDFLKAYHQNNNPNTLRTMLGKRVLDKTWKMMVQNAEEC 368

Query: 296 VDVPSTYNQSSKTDFVETNNQVLHEEPFNDNFLAD 326
           V VP   NQ      V+ N  V      +D+ + D
Sbjct: 369 V-VPIDNNQ-----IVQANGPVKFNHVMDDSAIKD 392

BLAST of Spg008436 vs. NCBI nr
Match: XP_004135591.1 (calmodulin-binding protein 60 G isoform X3 [Cucumis sativus])

HSP 1 Score: 267.7 bits (683), Expect = 1.6e-67
Identity = 150/275 (54.55%), Postives = 186/275 (67.64%), Query Frame = 0

Query: 56  RLRFSSRIAAEIFTNNKIRDEKGEALKVELICDV---GSSMAPSPLLLNAQIELFILENN 115
           RL F +   + IFTNN+I+ E GE LKV  ICD     S ++ SP L + Q+EL IL   
Sbjct: 129 RLFFLNSFPSVIFTNNEIKSEDGEPLKV-AICDTTNCNSIVSTSP-LSSVQVELCILPG- 188

Query: 116 TEKFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGSMENLTVTDN 175
             +F SSN+R++E  WT S F  +IL+PRDGKRPLIIG D ++YL++GVG + NL +TDN
Sbjct: 189 --EFDSSNRRDEETPWTSSYFNTSILTPRDGKRPLIIGNDRQVYLKDGVGFINNLIITDN 248

Query: 176 SSWTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPPSWEDPVWRL 235
           SSW K+K FRLGAKI D  I A F RI EAVSQPF+V D RGEVN+KHHPP  +D VWRL
Sbjct: 249 SSWMKSKKFRLGAKITDERIPAGFGRIGEAVSQPFRVMDQRGEVNQKHHPPRMDDEVWRL 308

Query: 236 EGIGKGGVYHTNLSNQGIYTVGDFLIALWR--NANHLRMLLGQRVLDSTWNKMIENAQEC 295
           EGI K G+YH NLS+QGI TVGDFL A  +  N N LR +LG+RVLD TW  M++NA+EC
Sbjct: 309 EGIAKYGIYHKNLSSQGIKTVGDFLKAYHQNNNPNTLRTMLGKRVLDKTWKMMVQNAEEC 368

Query: 296 VDVPSTYNQSSKTDFVETNNQVLHEEPFNDNFLAD 326
           V VP   NQ      V+ N  V      +D+ + D
Sbjct: 369 V-VPIDNNQ-----IVQANGPVKFNHVMDDSAIKD 392

BLAST of Spg008436 vs. ExPASy Swiss-Prot
Match: Q9FKL6 (Calmodulin-binding protein 60 B OS=Arabidopsis thaliana OX=3702 GN=CBP60B PE=2 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 9.6e-44
Identity = 101/307 (32.90%), Postives = 175/307 (57.00%), Query Frame = 0

Query: 56  RLRFSSRIAAEIFTNNKIRDEKGEALKVELI-CDVGSSMAPSPLLLNAQIELFILENNTE 115
           +L F SR++  +FT  K+  E+G  + V LI  + G ++   P   +A++ + +LE    
Sbjct: 95  QLHFKSRLSLPLFTGGKVEGEQGAVIHVVLIDANTGRAVVYGP-EASAKLHIVVLE---- 154

Query: 116 KFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGSMENLTVTDNSS 175
             G  N  +DE  WT  +F+++++  R GKRPL+ G +V + L+ GVG++  L  TDNSS
Sbjct: 155 --GDFNTEDDE-DWTQEEFESHVVKERSGKRPLLTG-EVYVTLKEGVGTLGELVFTDNSS 214

Query: 176 WTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPPSWEDPVWRLEG 235
           W +++ FRLG +++      D  RI+EA ++ F VKDHRGE+ KKH+PP+  D VWRL+ 
Sbjct: 215 WIRSRKFRLGLRVVSG--CCDGMRIREAKTEAFVVKDHRGELYKKHYPPALNDDVWRLDK 274

Query: 236 IGKGGVYHTNLSNQGIYTVGDFLIALWRNANHLRMLLGQRVLDSTWNKMIENAQECVDVP 295
           IGK G +H  L+ +GI TV DFL  + +++  LR +LG  + +  W+ ++E+A+ CV   
Sbjct: 275 IGKDGAFHKKLTAEGINTVEDFLRVMVKDSPKLRTILGSGMSNKMWDALVEHAKTCVQSS 334

Query: 296 STYNQSSK---------TDFVETNNQVLHEEPFNDNFLADELPYFCEDQPRMSMVNFNDL 353
             Y   ++          +  E +  +  ++ F+ + L D    + E   + +  N+N +
Sbjct: 335 KLYIYYAEDSRNVGVVFNNIYELSGLISGDQYFSADSLTDSQKVYVEGLVKKAYENWNLV 390

BLAST of Spg008436 vs. ExPASy Swiss-Prot
Match: C0SVV6 (Calmodulin-binding protein 60 A OS=Arabidopsis thaliana OX=3702 GN=CBP60A PE=2 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.8e-43
Identity = 92/236 (38.98%), Postives = 146/236 (61.86%), Query Frame = 0

Query: 56  RLRFSSRIAAEIFTNNKIRDEKGEALKVELICDVGSSMAPSPLLLNAQIELFILENNTEK 115
           +L+F + ++  +FT+ +I  ++G+A++V LI      +  S    +A++E+F++E     
Sbjct: 72  QLKFLNNLSLPVFTSARIEGDEGQAIRVGLIDPSTGQIFSSGPASSAKLEVFVVE----- 131

Query: 116 FGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGSMENLTVTDNSSW 175
            G  N   D   WT  D + NI+  R+GK+PL+ G +V   L +G+G M+ ++ TDNSSW
Sbjct: 132 -GDFNSVSD---WTDEDIRNNIVREREGKKPLLNG-NVFAVLNDGIGVMDEISFTDNSSW 191

Query: 176 TKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPPSWEDPVWRLEGI 235
           T+++ FRLG +I+D     D+ +I+EA+++ F V+DHRGE+ KKHHPPS  D VWRLE I
Sbjct: 192 TRSRKFRLGVRIVDQ---FDYVKIREAITESFVVRDHRGELYKKHHPPSLFDEVWRLEKI 251

Query: 236 GKGGVYHTNLSNQGIYTVGDFLIALWRNANHLRMLLGQRVLDSTWNKMIENAQECV 292
           GK G +H  L+   I TV DFL     N++ LR +LG  +    W   +++A+ CV
Sbjct: 252 GKDGAFHRRLNLSNINTVKDFLTHFHLNSSKLRQVLGTGMSSKMWEITLDHARSCV 294

BLAST of Spg008436 vs. ExPASy Swiss-Prot
Match: Q0WVV6 (Calmodulin-binding protein 60 D OS=Arabidopsis thaliana OX=3702 GN=CBP60D PE=2 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 3.1e-42
Identity = 101/307 (32.90%), Postives = 172/307 (56.03%), Query Frame = 0

Query: 56  RLRFSSRIAAEIFTNNKIRDEKGEALKVELI-CDVGSSMAPSPLLLNAQIELFILENNTE 115
           +L F SR++  +FT  ++  E+G  + V LI  + G  +   P   + ++E+ +L     
Sbjct: 84  QLHFKSRLSLPLFTGGRVEGEQGATIHVVLIDANTGRPVTVGP-EASLKLEVVVL----- 143

Query: 116 KFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGSMENLTVTDNSS 175
             G     ED+  WT  +F+++++  R+GKRPL+ G D+ + L+ GVG++  +  TDNSS
Sbjct: 144 --GGDFNNEDDEDWTQEEFESHVVKEREGKRPLLTG-DLFVVLKEGVGTLGEIVFTDNSS 203

Query: 176 WTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPPSWEDPVWRLEG 235
           W +++ FRLG ++       D  RI+EA ++ F VKDHRGE+ KKH+PP+  D VWRLE 
Sbjct: 204 WIRSRKFRLGLRVPSG--YCDGIRIREAKTEAFSVKDHRGELYKKHYPPALNDEVWRLEK 263

Query: 236 IGKGGVYHTNLSNQGIYTVGDFLIALWRNANHLRMLLGQRVLDSTWNKMIENAQECV--- 295
           IGK G +H  L+  GI TV  FL  L R++  LR +LG  + +  W+ ++E+A+ CV   
Sbjct: 264 IGKDGAFHKRLTAAGIVTVEGFLRQLVRDSTKLRAILGSGMSNKMWDLLVEHAKTCVLSG 323

Query: 296 DVPSTYNQSSKTDFVETNN------QVLHEEPFNDNFLADELPYFCEDQPRMSMVNFNDL 353
            +   Y + S++  V  NN       +  ++  + + L++    + +   + +  N+N +
Sbjct: 324 KLYIYYTEDSRSVGVVFNNIYELSGLITEDQYLSADSLSESQKVYVDGLVKKAYENWNQV 379

BLAST of Spg008436 vs. ExPASy Swiss-Prot
Match: C0SV51 (Calmodulin-binding protein 60 C OS=Arabidopsis thaliana OX=3702 GN=CBP60C PE=2 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 1.3e-40
Identity = 91/249 (36.55%), Postives = 151/249 (60.64%), Query Frame = 0

Query: 44  KYINTRAGRSAWRLRFSSRIAAEIFTNNKIRDEKGEALKVELI-CDVGSSMAPSPLLLNA 103
           K I    GR+  +L+F SR++  +FT  KI  E+G A+ V L+    G  +   P   +A
Sbjct: 88  KRIEGIGGRNL-QLQFRSRLSVPLFTGGKIEGEQGAAIHVVLLDMTTGHVLTVGP-EASA 147

Query: 104 QIELFILENNTEKFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVG 163
           ++++ +L+ +          ED+  W+  +F+ +++  R GKRPL+ G DV++ L+ GVG
Sbjct: 148 KLDVVVLDGDF-------NTEDDDGWSGEEFEGHLVKERQGKRPLLTG-DVQVTLKEGVG 207

Query: 164 SMENLTVTDNSSWTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHP 223
           ++  L  TDNSSW + + FRLG ++  ++   +  R++EA ++ F VKDHRGE+ KKH+P
Sbjct: 208 TLGELIFTDNSSWIRCRKFRLGLRV--SSGYCEGMRVREAKTEAFTVKDHRGELYKKHYP 267

Query: 224 PSWEDPVWRLEGIGKGGVYHTNLSNQGIYTVGDFLIALWRNANHLRMLLGQRVLDSTWNK 283
           P+ +D VWRLE IGK G +H  L+  GIY V +FL  + +++  LR +LG  + +  W  
Sbjct: 268 PALDDEVWRLEKIGKDGAFHKKLNKAGIYNVKEFLRLMVKDSQKLRTILGSGMSNRMWET 324

Query: 284 MIENAQECV 292
           + E+++ CV
Sbjct: 328 LAEHSKTCV 324

BLAST of Spg008436 vs. ExPASy Swiss-Prot
Match: F4IPM3 (Calmodulin-binding protein 60 E OS=Arabidopsis thaliana OX=3702 GN=CBP60E PE=2 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 8.4e-40
Identity = 96/248 (38.71%), Postives = 141/248 (56.85%), Query Frame = 0

Query: 44  KYINTRAGRSAWRLRFSSRIAAEIFTNNKIRDEKGEALKVELICDVGSSMAPSPLLLNAQ 103
           K I  R GR+  +L F +R+   +FT  K+  E+G A+ V LI     ++  +     ++
Sbjct: 79  KRIQDRNGRNL-QLHFRTRMPPHLFTGGKVEGERGSAIHVVLIDANTGNVVQTGEESASK 138

Query: 104 IELFILENNTEKFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGS 163
           + + +LE      G  N  +DE  WT   F++  +  R+GKRP++ G D ++ L+ GVG+
Sbjct: 139 LNVVVLE------GDFNDEDDE-DWTREHFESFEVKEREGKRPILTG-DTQIVLKEGVGT 198

Query: 164 MENLTVTDNSSWTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPP 223
           +  LT TDNSSW +++ FRLG K        D   I+EA ++PF VKDHRGE+ KKH+PP
Sbjct: 199 LGELTFTDNSSWIRSRKFRLGVKPASG--YGDSFCIREAKTEPFAVKDHRGELYKKHYPP 258

Query: 224 SWEDPVWRLEGIGKGGVYHTNLSNQGIYTVGDFLIALWRNANHLRMLLGQRVLDSTWNKM 283
           +  D VWRL+ I K GV H  L    I TV DFL  L ++   LR LLG  + +  W   
Sbjct: 259 AVHDEVWRLDRIAKDGVLHKKLLKANIVTVEDFLRLLVKDPQKLRNLLGSGMSNRMWENT 315

Query: 284 IENAQECV 292
           +E+A+ CV
Sbjct: 319 VEHAKTCV 315

BLAST of Spg008436 vs. ExPASy TrEMBL
Match: A0A1S3BQ76 (calmodulin-binding protein 60 G-like OS=Cucumis melo OX=3656 GN=LOC103492135 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 2.6e-68
Identity = 144/271 (53.14%), Postives = 189/271 (69.74%), Query Frame = 0

Query: 55  WRLRFSSRIAAEIFTNNKIRDEKGEALKVELICDV--GSSMAPSPLLLNAQIELFILENN 114
           ++L F +   + IFTNN+++ E GE LKV  ICD    +S+  +  L +AQ+EL IL   
Sbjct: 127 FKLCFLNSFPSVIFTNNEVKSEDGEPLKV-AICDATNSNSIVSTGPLSSAQVELCILPG- 186

Query: 115 TEKFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGSMENLTVTDN 174
             +F SSN+R++E  WT S F  +IL+PRDGKRPLIIG D ++YL++GV  + NL +TDN
Sbjct: 187 --EFDSSNRRDEETPWTSSYFNKSILTPRDGKRPLIIGDDRQVYLKDGVRLINNLIITDN 246

Query: 175 SSWTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPPSWEDPVWRL 234
           SSW K+K FRLG KI D  +LA+F RI EA+S+PF+V D RGEVN+KHHPP WED VWRL
Sbjct: 247 SSWMKSKKFRLGVKIKDERVLAEFVRIGEAISEPFRVMDQRGEVNQKHHPPRWEDEVWRL 306

Query: 235 EGIGKGGVYHTNLSNQGIYTVGDFLIALWRN-ANHLRMLLGQRVLDSTWNKMIENAQECV 294
           EGI K G+YH NLS++GI TVGDFL A  +N  + LRM+LG+RVLD TW  M++NA ECV
Sbjct: 307 EGIAKDGIYHKNLSSKGIRTVGDFLKAYHQNDPDTLRMMLGKRVLDKTWKMMVQNACECV 366

Query: 295 DVPSTYNQSSKTDFVETNNQVLHEEPFNDNF 323
           +     NQ  + +     NQVL +E F + F
Sbjct: 367 EDTFDNNQVFQPNSFLEFNQVL-DETFEETF 392

BLAST of Spg008436 vs. ExPASy TrEMBL
Match: A0A5D3CGF5 (Calmodulin-binding protein 60 G-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004240 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 2.6e-68
Identity = 144/271 (53.14%), Postives = 189/271 (69.74%), Query Frame = 0

Query: 55  WRLRFSSRIAAEIFTNNKIRDEKGEALKVELICDV--GSSMAPSPLLLNAQIELFILENN 114
           ++L F +   + IFTNN+++ E GE LKV  ICD    +S+  +  L +AQ+EL IL   
Sbjct: 127 FKLCFLNSFPSVIFTNNEVKSEDGEPLKV-AICDATNSNSIVSTGPLSSAQVELCILPG- 186

Query: 115 TEKFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGSMENLTVTDN 174
             +F SSN+R++E  WT S F  +IL+PRDGKRPLIIG D ++YL++GV  + NL +TDN
Sbjct: 187 --EFDSSNRRDEETPWTSSYFNKSILTPRDGKRPLIIGDDRQVYLKDGVRLINNLIITDN 246

Query: 175 SSWTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPPSWEDPVWRL 234
           SSW K+K FRLG KI D  +LA+F RI EA+S+PF+V D RGEVN+KHHPP WED VWRL
Sbjct: 247 SSWMKSKKFRLGVKIKDERVLAEFVRIGEAISEPFRVMDQRGEVNQKHHPPRWEDEVWRL 306

Query: 235 EGIGKGGVYHTNLSNQGIYTVGDFLIALWRN-ANHLRMLLGQRVLDSTWNKMIENAQECV 294
           EGI K G+YH NLS++GI TVGDFL A  +N  + LRM+LG+RVLD TW  M++NA ECV
Sbjct: 307 EGIAKDGIYHKNLSSKGIRTVGDFLKAYHQNDPDTLRMMLGKRVLDKTWKMMVQNACECV 366

Query: 295 DVPSTYNQSSKTDFVETNNQVLHEEPFNDNF 323
           +     NQ  + +     NQVL +E F + F
Sbjct: 367 EDTFDNNQVFQPNSFLEFNQVL-DETFEETF 392

BLAST of Spg008436 vs. ExPASy TrEMBL
Match: A0A6J1HVQ8 (calmodulin-binding protein 60 G-like OS=Cucurbita maxima OX=3661 GN=LOC111467884 PE=3 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 4.8e-62
Identity = 144/339 (42.48%), Postives = 213/339 (62.83%), Query Frame = 0

Query: 50  AGRSAWRLRFSSRIAAEIFTNNKIRDEKGEALKVELICDVG--SSMAPSPLLLNAQIELF 109
           +G    +L F +R+ + I+T N I  E  E L++ L CD    +++ P+  LL+A IE  
Sbjct: 83  SGAVKLKLHFMNRVPSFIYTTNDIEAENNEPLRIAL-CDPTNCNNIDPTDPLLSALIEFV 142

Query: 110 ILENNTEKFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGSMENL 169
           ++    +    S QR+DE++W++ +FK NIL  R+GKRPL++G D+++ LENGV  +++L
Sbjct: 143 VINGEFD----STQRDDEVHWSVDEFKKNILIEREGKRPLLVGNDLRVRLENGVAVIKHL 202

Query: 170 TVTDNSSWTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPPSWED 229
             TDNSSW ++K FRLG +IIDN+ILA +PRI EA+S PF+V D+RG+VNKK+HPP  ED
Sbjct: 203 RFTDNSSWRRSKMFRLGVRIIDNDILAKYPRIGEAISNPFRVMDNRGQVNKKNHPPKNED 262

Query: 230 PVWRLEGIGKGGVYHTNLSNQGIYTVGDFLIALWRNANHLRMLLGQRVLDSTWNKMIENA 289
            VWRLEGIGK G YH NLS++GI  V +F  A  R++  LR LLG +V + TW  M++NA
Sbjct: 263 EVWRLEGIGKDGKYHKNLSSKGIKNVEEFRNAYKRDSCSLRKLLGDKVPNKTWKAMVDNA 322

Query: 290 QECVDVPSTYNQSSKTDFVETNNQVLHEEPFNDNFLADELPYFCEDQPRMSMVNFNDLVT 349
            EC D P+T  Q+ +      N Q++     +D+    +  +        ++ +F  L++
Sbjct: 323 LEC-DSPTTCYQNLEVG----NPQII----ISDSHGLPQTGFDGYQGYDFTIEDFQSLLS 382

Query: 350 PNGEASTSHEPDYTN-TQSGNTYYQGVEPSYCPNPPHPN 386
            +G+A TS   +Y N T   N Y Q  EPS+C NP + N
Sbjct: 383 -HGQALTS---NYRNDTHMYNQYNQQQEPSWCLNPSNEN 403

BLAST of Spg008436 vs. ExPASy TrEMBL
Match: A0A6J1HED3 (calmodulin-binding protein 60 A-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462098 PE=3 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 1.1e-61
Identity = 139/349 (39.83%), Postives = 218/349 (62.46%), Query Frame = 0

Query: 38  GEDEIGKYINTRAGRSAWRLRFSSRIAAEIFTNNKIRDEKGEALKVELICDVG-SSMAPS 97
           GE+++G+    +      +LRF ++I   IFTN+ +  E GE L++ L      + + P+
Sbjct: 69  GEEDVGESRTVKL-----KLRFMNKIPPVIFTNDDVEAENGEPLRIALFDPTNCNDIDPT 128

Query: 98  PLLLNAQIELFILENNTEKFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLY 157
             LL+A IE  +++   +    S QR+DE++W++ +FK  IL  R+GKRPL++G D+++ 
Sbjct: 129 DPLLSALIEFVVIDGEFD----STQRDDEVHWSVDEFKKGILIEREGKRPLLVGNDLRVG 188

Query: 158 LENGVGSMENLTVTDNSSWTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEV 217
           LENGV  +++L  TDNSSW ++K FRLG +IIDN+ILA +PRI EA+S+PF+V D+RG+V
Sbjct: 189 LENGVAVIKHLRFTDNSSWRRSKTFRLGVRIIDNDILAKYPRIGEAISKPFRVMDNRGQV 248

Query: 218 NKKHHPPSWEDPVWRLEGIGKGGVYHTNLSNQGIYTVGDFLIALWRNANHLRMLLGQRVL 277
            KK+HPP  ED VWRL+GIGK G YH +LS++GI +V +F  A  +++  LR LLG +V 
Sbjct: 249 IKKNHPPKGEDEVWRLKGIGKDGKYHKSLSSKGIKSVEEFKNAYKKDSRSLRKLLGNKVP 308

Query: 278 DSTWNKMIENAQECVDVPSTYNQSSKTDFVETNNQVLHEEPFNDNFLADELPYFCEDQPR 337
           + TW  M++NA EC D P+T  Q+ +      N Q++     +D+ +  +  +       
Sbjct: 309 NKTWKAMVDNALEC-DSPTTCYQTLEVG----NPQII----ISDSHVLPQTGFDGYQGYD 368

Query: 338 MSMVNFNDLVTPNGEASTSHEPDYTNTQSGNTYYQGVEPSYCPNPPHPN 386
            ++ +F  L++ +GEA TS+  + TN       Y   EPS+C NP + N
Sbjct: 369 FTLEDFQSLLS-HGEALTSNNRNDTNMYD---RYNQQEPSWCLNPSNEN 395

BLAST of Spg008436 vs. ExPASy TrEMBL
Match: A0A6J1HC45 (calmodulin-binding protein 60 A-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462098 PE=3 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 1.1e-61
Identity = 139/349 (39.83%), Postives = 218/349 (62.46%), Query Frame = 0

Query: 38  GEDEIGKYINTRAGRSAWRLRFSSRIAAEIFTNNKIRDEKGEALKVELICDVG-SSMAPS 97
           GE+++G+    +      +LRF ++I   IFTN+ +  E GE L++ L      + + P+
Sbjct: 76  GEEDVGESRTVKL-----KLRFMNKIPPVIFTNDDVEAENGEPLRIALFDPTNCNDIDPT 135

Query: 98  PLLLNAQIELFILENNTEKFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLY 157
             LL+A IE  +++   +    S QR+DE++W++ +FK  IL  R+GKRPL++G D+++ 
Sbjct: 136 DPLLSALIEFVVIDGEFD----STQRDDEVHWSVDEFKKGILIEREGKRPLLVGNDLRVG 195

Query: 158 LENGVGSMENLTVTDNSSWTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEV 217
           LENGV  +++L  TDNSSW ++K FRLG +IIDN+ILA +PRI EA+S+PF+V D+RG+V
Sbjct: 196 LENGVAVIKHLRFTDNSSWRRSKTFRLGVRIIDNDILAKYPRIGEAISKPFRVMDNRGQV 255

Query: 218 NKKHHPPSWEDPVWRLEGIGKGGVYHTNLSNQGIYTVGDFLIALWRNANHLRMLLGQRVL 277
            KK+HPP  ED VWRL+GIGK G YH +LS++GI +V +F  A  +++  LR LLG +V 
Sbjct: 256 IKKNHPPKGEDEVWRLKGIGKDGKYHKSLSSKGIKSVEEFKNAYKKDSRSLRKLLGNKVP 315

Query: 278 DSTWNKMIENAQECVDVPSTYNQSSKTDFVETNNQVLHEEPFNDNFLADELPYFCEDQPR 337
           + TW  M++NA EC D P+T  Q+ +      N Q++     +D+ +  +  +       
Sbjct: 316 NKTWKAMVDNALEC-DSPTTCYQTLEVG----NPQII----ISDSHVLPQTGFDGYQGYD 375

Query: 338 MSMVNFNDLVTPNGEASTSHEPDYTNTQSGNTYYQGVEPSYCPNPPHPN 386
            ++ +F  L++ +GEA TS+  + TN       Y   EPS+C NP + N
Sbjct: 376 FTLEDFQSLLS-HGEALTSNNRNDTNMYD---RYNQQEPSWCLNPSNEN 402

BLAST of Spg008436 vs. TAIR 10
Match: AT5G57580.1 (Calmodulin-binding protein )

HSP 1 Score: 179.1 bits (453), Expect = 6.8e-45
Identity = 101/307 (32.90%), Postives = 175/307 (57.00%), Query Frame = 0

Query: 56  RLRFSSRIAAEIFTNNKIRDEKGEALKVELI-CDVGSSMAPSPLLLNAQIELFILENNTE 115
           +L F SR++  +FT  K+  E+G  + V LI  + G ++   P   +A++ + +LE    
Sbjct: 95  QLHFKSRLSLPLFTGGKVEGEQGAVIHVVLIDANTGRAVVYGP-EASAKLHIVVLE---- 154

Query: 116 KFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGSMENLTVTDNSS 175
             G  N  +DE  WT  +F+++++  R GKRPL+ G +V + L+ GVG++  L  TDNSS
Sbjct: 155 --GDFNTEDDE-DWTQEEFESHVVKERSGKRPLLTG-EVYVTLKEGVGTLGELVFTDNSS 214

Query: 176 WTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPPSWEDPVWRLEG 235
           W +++ FRLG +++      D  RI+EA ++ F VKDHRGE+ KKH+PP+  D VWRL+ 
Sbjct: 215 WIRSRKFRLGLRVVSG--CCDGMRIREAKTEAFVVKDHRGELYKKHYPPALNDDVWRLDK 274

Query: 236 IGKGGVYHTNLSNQGIYTVGDFLIALWRNANHLRMLLGQRVLDSTWNKMIENAQECVDVP 295
           IGK G +H  L+ +GI TV DFL  + +++  LR +LG  + +  W+ ++E+A+ CV   
Sbjct: 275 IGKDGAFHKKLTAEGINTVEDFLRVMVKDSPKLRTILGSGMSNKMWDALVEHAKTCVQSS 334

Query: 296 STYNQSSK---------TDFVETNNQVLHEEPFNDNFLADELPYFCEDQPRMSMVNFNDL 353
             Y   ++          +  E +  +  ++ F+ + L D    + E   + +  N+N +
Sbjct: 335 KLYIYYAEDSRNVGVVFNNIYELSGLISGDQYFSADSLTDSQKVYVEGLVKKAYENWNLV 390

BLAST of Spg008436 vs. TAIR 10
Match: AT5G62570.1 (Calmodulin binding protein-like )

HSP 1 Score: 177.6 bits (449), Expect = 2.0e-44
Identity = 92/236 (38.98%), Postives = 146/236 (61.86%), Query Frame = 0

Query: 56  RLRFSSRIAAEIFTNNKIRDEKGEALKVELICDVGSSMAPSPLLLNAQIELFILENNTEK 115
           +L+F + ++  +FT+ +I  ++G+A++V LI      +  S    +A++E+F++E     
Sbjct: 54  QLKFLNNLSLPVFTSARIEGDEGQAIRVGLIDPSTGQIFSSGPASSAKLEVFVVE----- 113

Query: 116 FGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGSMENLTVTDNSSW 175
            G  N   D   WT  D + NI+  R+GK+PL+ G +V   L +G+G M+ ++ TDNSSW
Sbjct: 114 -GDFNSVSD---WTDEDIRNNIVREREGKKPLLNG-NVFAVLNDGIGVMDEISFTDNSSW 173

Query: 176 TKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPPSWEDPVWRLEGI 235
           T+++ FRLG +I+D     D+ +I+EA+++ F V+DHRGE+ KKHHPPS  D VWRLE I
Sbjct: 174 TRSRKFRLGVRIVDQ---FDYVKIREAITESFVVRDHRGELYKKHHPPSLFDEVWRLEKI 233

Query: 236 GKGGVYHTNLSNQGIYTVGDFLIALWRNANHLRMLLGQRVLDSTWNKMIENAQECV 292
           GK G +H  L+   I TV DFL     N++ LR +LG  +    W   +++A+ CV
Sbjct: 234 GKDGAFHRRLNLSNINTVKDFLTHFHLNSSKLRQVLGTGMSSKMWEITLDHARSCV 276

BLAST of Spg008436 vs. TAIR 10
Match: AT5G62570.2 (Calmodulin binding protein-like )

HSP 1 Score: 177.6 bits (449), Expect = 2.0e-44
Identity = 92/236 (38.98%), Postives = 146/236 (61.86%), Query Frame = 0

Query: 56  RLRFSSRIAAEIFTNNKIRDEKGEALKVELICDVGSSMAPSPLLLNAQIELFILENNTEK 115
           +L+F + ++  +FT+ +I  ++G+A++V LI      +  S    +A++E+F++E     
Sbjct: 72  QLKFLNNLSLPVFTSARIEGDEGQAIRVGLIDPSTGQIFSSGPASSAKLEVFVVE----- 131

Query: 116 FGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGSMENLTVTDNSSW 175
            G  N   D   WT  D + NI+  R+GK+PL+ G +V   L +G+G M+ ++ TDNSSW
Sbjct: 132 -GDFNSVSD---WTDEDIRNNIVREREGKKPLLNG-NVFAVLNDGIGVMDEISFTDNSSW 191

Query: 176 TKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPPSWEDPVWRLEGI 235
           T+++ FRLG +I+D     D+ +I+EA+++ F V+DHRGE+ KKHHPPS  D VWRLE I
Sbjct: 192 TRSRKFRLGVRIVDQ---FDYVKIREAITESFVVRDHRGELYKKHHPPSLFDEVWRLEKI 251

Query: 236 GKGGVYHTNLSNQGIYTVGDFLIALWRNANHLRMLLGQRVLDSTWNKMIENAQECV 292
           GK G +H  L+   I TV DFL     N++ LR +LG  +    W   +++A+ CV
Sbjct: 252 GKDGAFHRRLNLSNINTVKDFLTHFHLNSSKLRQVLGTGMSSKMWEITLDHARSCV 294

BLAST of Spg008436 vs. TAIR 10
Match: AT4G25800.1 (Calmodulin-binding protein )

HSP 1 Score: 174.1 bits (440), Expect = 2.2e-43
Identity = 101/307 (32.90%), Postives = 172/307 (56.03%), Query Frame = 0

Query: 56  RLRFSSRIAAEIFTNNKIRDEKGEALKVELI-CDVGSSMAPSPLLLNAQIELFILENNTE 115
           +L F SR++  +FT  ++  E+G  + V LI  + G  +   P   + ++E+ +L     
Sbjct: 84  QLHFKSRLSLPLFTGGRVEGEQGATIHVVLIDANTGRPVTVGP-EASLKLEVVVL----- 143

Query: 116 KFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGSMENLTVTDNSS 175
             G     ED+  WT  +F+++++  R+GKRPL+ G D+ + L+ GVG++  +  TDNSS
Sbjct: 144 --GGDFNNEDDEDWTQEEFESHVVKEREGKRPLLTG-DLFVVLKEGVGTLGEIVFTDNSS 203

Query: 176 WTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPPSWEDPVWRLEG 235
           W +++ FRLG ++       D  RI+EA ++ F VKDHRGE+ KKH+PP+  D VWRLE 
Sbjct: 204 WIRSRKFRLGLRVPSG--YCDGIRIREAKTEAFSVKDHRGELYKKHYPPALNDEVWRLEK 263

Query: 236 IGKGGVYHTNLSNQGIYTVGDFLIALWRNANHLRMLLGQRVLDSTWNKMIENAQECV--- 295
           IGK G +H  L+  GI TV  FL  L R++  LR +LG  + +  W+ ++E+A+ CV   
Sbjct: 264 IGKDGAFHKRLTAAGIVTVEGFLRQLVRDSTKLRAILGSGMSNKMWDLLVEHAKTCVLSG 323

Query: 296 DVPSTYNQSSKTDFVETNN------QVLHEEPFNDNFLADELPYFCEDQPRMSMVNFNDL 353
            +   Y + S++  V  NN       +  ++  + + L++    + +   + +  N+N +
Sbjct: 324 KLYIYYTEDSRSVGVVFNNIYELSGLITEDQYLSADSLSESQKVYVDGLVKKAYENWNQV 379

BLAST of Spg008436 vs. TAIR 10
Match: AT4G25800.2 (Calmodulin-binding protein )

HSP 1 Score: 174.1 bits (440), Expect = 2.2e-43
Identity = 101/307 (32.90%), Postives = 172/307 (56.03%), Query Frame = 0

Query: 56  RLRFSSRIAAEIFTNNKIRDEKGEALKVELI-CDVGSSMAPSPLLLNAQIELFILENNTE 115
           +L F SR++  +FT  ++  E+G  + V LI  + G  +   P   + ++E+ +L     
Sbjct: 84  QLHFKSRLSLPLFTGGRVEGEQGATIHVVLIDANTGRPVTVGP-EASLKLEVVVL----- 143

Query: 116 KFGSSNQREDEIYWTLSDFKANILSPRDGKRPLIIGTDVKLYLENGVGSMENLTVTDNSS 175
             G     ED+  WT  +F+++++  R+GKRPL+ G D+ + L+ GVG++  +  TDNSS
Sbjct: 144 --GGDFNNEDDEDWTQEEFESHVVKEREGKRPLLTG-DLFVVLKEGVGTLGEIVFTDNSS 203

Query: 176 WTKTKCFRLGAKIIDNNILADFPRIQEAVSQPFQVKDHRGEVNKKHHPPSWEDPVWRLEG 235
           W +++ FRLG ++       D  RI+EA ++ F VKDHRGE+ KKH+PP+  D VWRLE 
Sbjct: 204 WIRSRKFRLGLRVPSG--YCDGIRIREAKTEAFSVKDHRGELYKKHYPPALNDEVWRLEK 263

Query: 236 IGKGGVYHTNLSNQGIYTVGDFLIALWRNANHLRMLLGQRVLDSTWNKMIENAQECV--- 295
           IGK G +H  L+  GI TV  FL  L R++  LR +LG  + +  W+ ++E+A+ CV   
Sbjct: 264 IGKDGAFHKRLTAAGIVTVEGFLRQLVRDSTKLRAILGSGMSNKMWDLLVEHAKTCVLSG 323

Query: 296 DVPSTYNQSSKTDFVETNN------QVLHEEPFNDNFLADELPYFCEDQPRMSMVNFNDL 353
            +   Y + S++  V  NN       +  ++  + + L++    + +   + +  N+N +
Sbjct: 324 KLYIYYTEDSRSVGVVFNNIYELSGLITEDQYLSADSLSESQKVYVDGLVKKAYENWNQV 379

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879916.16.4e-6950.00calmodulin-binding protein 60 G-like isoform X1 [Benincasa hispida][more]
XP_038879917.12.4e-6853.99calmodulin-binding protein 60 G-like isoform X2 [Benincasa hispida][more]
XP_008450592.15.4e-6853.14PREDICTED: calmodulin-binding protein 60 G-like [Cucumis melo] >XP_008450593.1 P... [more]
XP_031743132.11.6e-6754.55calmodulin-binding protein 60 G isoform X1 [Cucumis sativus][more]
XP_004135591.11.6e-6754.55calmodulin-binding protein 60 G isoform X3 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q9FKL69.6e-4432.90Calmodulin-binding protein 60 B OS=Arabidopsis thaliana OX=3702 GN=CBP60B PE=2 S... [more]
C0SVV62.8e-4338.98Calmodulin-binding protein 60 A OS=Arabidopsis thaliana OX=3702 GN=CBP60A PE=2 S... [more]
Q0WVV63.1e-4232.90Calmodulin-binding protein 60 D OS=Arabidopsis thaliana OX=3702 GN=CBP60D PE=2 S... [more]
C0SV511.3e-4036.55Calmodulin-binding protein 60 C OS=Arabidopsis thaliana OX=3702 GN=CBP60C PE=2 S... [more]
F4IPM38.4e-4038.71Calmodulin-binding protein 60 E OS=Arabidopsis thaliana OX=3702 GN=CBP60E PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A1S3BQ762.6e-6853.14calmodulin-binding protein 60 G-like OS=Cucumis melo OX=3656 GN=LOC103492135 PE=... [more]
A0A5D3CGF52.6e-6853.14Calmodulin-binding protein 60 G-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A6J1HVQ84.8e-6242.48calmodulin-binding protein 60 G-like OS=Cucurbita maxima OX=3661 GN=LOC111467884... [more]
A0A6J1HED31.1e-6139.83calmodulin-binding protein 60 A-like isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1HC451.1e-6139.83calmodulin-binding protein 60 A-like isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT5G57580.16.8e-4532.90Calmodulin-binding protein [more]
AT5G62570.12.0e-4438.98Calmodulin binding protein-like [more]
AT5G62570.22.0e-4438.98Calmodulin binding protein-like [more]
AT4G25800.12.2e-4332.90Calmodulin-binding protein [more]
AT4G25800.22.2e-4332.90Calmodulin-binding protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012416CALMODULIN-BINDING PROTEIN60PFAMPF07887Calmodulin_bindcoord: 55..297
e-value: 3.2E-69
score: 233.5
IPR012416CALMODULIN-BINDING PROTEIN60PANTHERPTHR31713OS02G0177800 PROTEINcoord: 52..367
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..43
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 348..388
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 348..374

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg008436.1Spg008436.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005516 calmodulin binding