Spg007221 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg007221
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionSulfate adenylyltransferase
Locationscaffold9: 47070205 .. 47073325 (-)
RNA-Seq ExpressionSpg007221
SyntenySpg007221
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGCGGTGGGGATTTATTTTGGAATGACCATTATGCTTTCTTTGGATTATCAGCAGATTCTCAGATAATCAAAAGTTTAGTGTAAAAATTAAAAGCAATGGATGTAAGCGTAAGAGAATATTTTGCAGCCAAGCACGTCGGGCCGCTCATAAACATTCTTCAGGAGTTATATTCTCTTTCTCTCTCTCTCTAAGCGTTGCTTTCAAAGAAGAATCGTTGTGGTTTCGGCCCATCATCACAACCACAACCATAGCCACAAGTCTTCTAAGTCTCAAACGCAGCGAGAGAGATAGCCGATTAGCCGCAACCAAAGCCACCCTCCCCAAGCGCAAAAGGCCACAAACAAACGTGTGGATCTCTACGGACGAGCCAATTTTCTTGGTTCCCTTTTCCCCACAACCATGTTTATGTTCTCAGTTCTTACAGATACACTTCCCCCATTTCTCCATTTTCGTCGGACCTTCAATCCTTTTTTGGCAATATCAAAACCCATCTCCGGAATTTCCCCTTTGAGCCCAAGATGTCTCTAGTCACTAAACTCCACATCGCCACTTCTCCCCAAATCAGTCTCAACACCCTTCAAACTTCAATAAAGAGAACGCCGATTATCACTCTCAACACCAATATCAAATCCAAACCCATTTACGTTTCTGAGCCTCTGGTGTTTAAACCCGCAATGCAGGGCTTTTCGCCTCGTTCTGTTCGCTCTTCGATTAAGAGCTCGCTGATCGAGCCCGACGGTGGGGCTGTTGTCGAGTTGGTTGTGCCTGAGAGTGAAAGGGCCTCGAGGGTTTTGGAGGCCGAGCCATTGCCGAAGGTGAAATTGACCCAGATCGATCTCGAGTGGGTTCATGTCATTTGTGAAGGATGGGCGAGTCCGTTGAAAGGGTTCATGCGAGAGGAGGAGTATTTGCAGAGTTTGCATTTCAATTGCTTGAAAATTAAAGATGGGTCTCTTGTCAACATGTCGCTTCCCATTGTTCTGGCCATTGACGACGATGTTAAGGAACAAATTGGGTCCTCGTCCAATGTGGGGTTGCTCGGTCCCAATGGAGACTTGGTTGGCATTCTTCGCAGGTTAGTTTTGTTGTTTAATTCCATTTGATCTTCGAAGTCAGTGTAGATGGAGATGGCGTTTTGTGGAATAGCGCATATGATATGACATAAACCTGTTTATTTAAATCCCGAGGTATGCTTTGTTATAATATTAATAATAATAATAATACTCTTCCACTTGCTCGCAACAACTTTGTTGATCATGAGATGTTAAATTGGAGGACAATATTTTAATCATACTCTGCTCGTTTGTTAGAAGATCTCTGGTTATTTGAAGTTCAAAACAAAGGTAACAATCTTGATGATCTTAGATTTCTGGGTTTAGAAATACAAACTGAAACTTTCTTTTTATTTGAACAGCGAGACCAATGTGTACAGAAATATGTAGTCAAACTGAAACAAAAAAATGTGTTTTTTCGAACCTCACTTTGAATTATTCTGCAGCATTGAGATATTCAAGCATAACAAGGAAGAGAGAATAGCTAGAACTTGGGGGACAACTGCACCAGGGCTGCCGTATGTTGATGAGGTCATTACAAATGCTGGAAACTGGCTTATTGGCGGTGATCTTGAAGTAATAAACCCCATCAAATACAACGATGGACTTGATCATTACCGGCTGTCTCCGAAACAACTTCGGCAAGAGTTCGATAGGCGTCAGGCTGATGCTGTTTTTGCCTTTCAATTGAGGAATCCGGTGCACAATGGGCATGCTTTGTTGATGAACGATACGCGAAAACGACTTTTGGACATGGGCTATAAGAATCCAATTCTATTGCTTCATCCTTTGGGAGGTTTCACAAAGGCAGATGATGTTCCATTGGATGTTCGAATGGAGCAACATAGCAAGGTTTGTTTTTCATATCAACAGTTTTCCATTAACATCATTCTGAATTGTTCTTTAACATCCAAAAACTTCCCCACCTTCATACTCTTTGGAATTATAAATGAAGCATTGTCTAACTTTTCCAACATTCGATAACCGAGATCGGTAAGCAACTTGAATAGGAAGTTAGTTGAATTACACGATTCATCTCTACTCTTCGGTTTATATGCGTGCTCGATTTTGATGCTTAGTTTGTGTTACTTAAGATAGACTTTCTGTTTTTAGGTTTTAGAAGATGGAGTTCTTGACCCTGAAACGACCATCGTCTCCATTTTCCCGTCGCCAATGCATTACGCAGGTCCAACCGAAGTACAATGGCATGCGAAAGCAAGGATCAATGCAGGTGCAAATTTCTACATTGTTGGTCGAGACCCTGCTGGAATGGGTCACCCAACAGAGAAGAGAGATTTATATGATCCAGACCATGGCAAGAAGGTCTTGAGTATGGCCCCTGGCCTTGAGAAGCTAAACATATTGCCCTTCAAGGTCAGTATCTCAATGACCCTCCCTTCTCCTTTTCCCACTTTCCACCCTCAGCATTTTAACATCCTGTCTTCCACAGGTGGCAGCATACGATACCGTGGCGAAAAAGATGGCGTTCTTCGAACCTTCGCGTGCAAAAGATTTCCTCTTCATCTCCGGAACAAAGGTAAGAGCTGAGTTGTTTTTGAACAGGATGTCATATGCATTGTGTGGCAACATAGTAACTGCATTGTTTGAATCTTTTGTGCATATGTTTTGTGCTAGATGCGGACTTACGCAAGGACCGGCGAGAACCCTCCCGACGGTTTCATGTGCCCCGGCGGATGGAAGGTGTTGGTCAACTACTACGAGAGTTTGCAGGCTGAAGACGCATCGCCTCAGCAACCTGCATTATCAGTTTAGGAGAGGATAGTTTGAGTTCATTTGAATGAGTAGTAGCAAATCTATGCGGGTGGATTGAGGAAGCTGCCGAGGATTTGACTAGAACACAATGCAACTTCAATTGTGTATAATCCATGCTTTCTTCTATTCTCGGTTTTTATGCTGTTTAGAATTTTTCCCCTTCGAATAGTGAATTATTTGTCATTTATCTTCCCTTGAGAAATGCTATTGTTATATTTTTTTATCCTCTTTTACTTCCATAACTATTAGCAACTATCAATTTCGTATGTTTTATAATAGAATTTCTCTGTTAACC

mRNA sequence

ATGTCTCTAGTCACTAAACTCCACATCGCCACTTCTCCCCAAATCAGTCTCAACACCCTTCAAACTTCAATAAAGAGAACGCCGATTATCACTCTCAACACCAATATCAAATCCAAACCCATTTACGTTTCTGAGCCTCTGGTGTTTAAACCCGCAATGCAGGGCTTTTCGCCTCGTTCTGTTCGCTCTTCGATTAAGAGCTCGCTGATCGAGCCCGACGGTGGGGCTGTTGTCGAGTTGGTTGTGCCTGAGAGTGAAAGGGCCTCGAGGGTTTTGGAGGCCGAGCCATTGCCGAAGGTGAAATTGACCCAGATCGATCTCGAGTGGGTTCATGTCATTTGTGAAGGATGGGCGAGTCCGTTGAAAGGGTTCATGCGAGAGGAGGAGTATTTGCAGAGTTTGCATTTCAATTGCTTGAAAATTAAAGATGGGTCTCTTGTCAACATGTCGCTTCCCATTGTTCTGGCCATTGACGACGATGTTAAGGAACAAATTGGGTCCTCGTCCAATGTGGGGTTGCTCGGTCCCAATGGAGACTTGGTTGGCATTCTTCGCAGCATTGAGATATTCAAGCATAACAAGGAAGAGAGAATAGCTAGAACTTGGGGGACAACTGCACCAGGGCTGCCGTATGTTGATGAGGTCATTACAAATGCTGGAAACTGGCTTATTGGCGGTGATCTTGAAGTAATAAACCCCATCAAATACAACGATGGACTTGATCATTACCGGCTGTCTCCGAAACAACTTCGGCAAGAGTTCGATAGGCGTCAGGCTGATGCTGTTTTTGCCTTTCAATTGAGGAATCCGGTGCACAATGGGCATGCTTTGTTGATGAACGATACGCGAAAACGACTTTTGGACATGGGCTATAAGAATCCAATTCTATTGCTTCATCCTTTGGGAGGTTTCACAAAGGCAGATGATGTTCCATTGGATGTTCGAATGGAGCAACATAGCAAGGTTTTAGAAGATGGAGTTCTTGACCCTGAAACGACCATCGTCTCCATTTTCCCGTCGCCAATGCATTACGCAGGTCCAACCGAAGTACAATGGCATGCGAAAGCAAGGATCAATGCAGGTGCAAATTTCTACATTGTTGGTCGAGACCCTGCTGGAATGGGTCACCCAACAGAGAAGAGAGATTTATATGATCCAGACCATGGCAAGAAGGTCTTGAGTATGGCCCCTGGCCTTGAGAAGCTAAACATATTGCCCTTCAAGGTGGCAGCATACGATACCGTGGCGAAAAAGATGGCGTTCTTCGAACCTTCGCGTGCAAAAGATTTCCTCTTCATCTCCGGAACAAAGATGCGGACTTACGCAAGGACCGGCGAGAACCCTCCCGACGGTTTCATGTGCCCCGGCGGATGGAAGGTGTTGGTCAACTACTACGAGAGTTTGCAGGCTGAAGACGCATCGCCTCAGCAACCTGCATTATCAGTTTAG

Coding sequence (CDS)

ATGTCTCTAGTCACTAAACTCCACATCGCCACTTCTCCCCAAATCAGTCTCAACACCCTTCAAACTTCAATAAAGAGAACGCCGATTATCACTCTCAACACCAATATCAAATCCAAACCCATTTACGTTTCTGAGCCTCTGGTGTTTAAACCCGCAATGCAGGGCTTTTCGCCTCGTTCTGTTCGCTCTTCGATTAAGAGCTCGCTGATCGAGCCCGACGGTGGGGCTGTTGTCGAGTTGGTTGTGCCTGAGAGTGAAAGGGCCTCGAGGGTTTTGGAGGCCGAGCCATTGCCGAAGGTGAAATTGACCCAGATCGATCTCGAGTGGGTTCATGTCATTTGTGAAGGATGGGCGAGTCCGTTGAAAGGGTTCATGCGAGAGGAGGAGTATTTGCAGAGTTTGCATTTCAATTGCTTGAAAATTAAAGATGGGTCTCTTGTCAACATGTCGCTTCCCATTGTTCTGGCCATTGACGACGATGTTAAGGAACAAATTGGGTCCTCGTCCAATGTGGGGTTGCTCGGTCCCAATGGAGACTTGGTTGGCATTCTTCGCAGCATTGAGATATTCAAGCATAACAAGGAAGAGAGAATAGCTAGAACTTGGGGGACAACTGCACCAGGGCTGCCGTATGTTGATGAGGTCATTACAAATGCTGGAAACTGGCTTATTGGCGGTGATCTTGAAGTAATAAACCCCATCAAATACAACGATGGACTTGATCATTACCGGCTGTCTCCGAAACAACTTCGGCAAGAGTTCGATAGGCGTCAGGCTGATGCTGTTTTTGCCTTTCAATTGAGGAATCCGGTGCACAATGGGCATGCTTTGTTGATGAACGATACGCGAAAACGACTTTTGGACATGGGCTATAAGAATCCAATTCTATTGCTTCATCCTTTGGGAGGTTTCACAAAGGCAGATGATGTTCCATTGGATGTTCGAATGGAGCAACATAGCAAGGTTTTAGAAGATGGAGTTCTTGACCCTGAAACGACCATCGTCTCCATTTTCCCGTCGCCAATGCATTACGCAGGTCCAACCGAAGTACAATGGCATGCGAAAGCAAGGATCAATGCAGGTGCAAATTTCTACATTGTTGGTCGAGACCCTGCTGGAATGGGTCACCCAACAGAGAAGAGAGATTTATATGATCCAGACCATGGCAAGAAGGTCTTGAGTATGGCCCCTGGCCTTGAGAAGCTAAACATATTGCCCTTCAAGGTGGCAGCATACGATACCGTGGCGAAAAAGATGGCGTTCTTCGAACCTTCGCGTGCAAAAGATTTCCTCTTCATCTCCGGAACAAAGATGCGGACTTACGCAAGGACCGGCGAGAACCCTCCCGACGGTTTCATGTGCCCCGGCGGATGGAAGGTGTTGGTCAACTACTACGAGAGTTTGCAGGCTGAAGACGCATCGCCTCAGCAACCTGCATTATCAGTTTAG

Protein sequence

MSLVTKLHIATSPQISLNTLQTSIKRTPIITLNTNIKSKPIYVSEPLVFKPAMQGFSPRSVRSSIKSSLIEPDGGAVVELVVPESERASRVLEAEPLPKVKLTQIDLEWVHVICEGWASPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYNDGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKMAFFEPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEDASPQQPALSV
Homology
BLAST of Spg007221 vs. NCBI nr
Match: XP_023525367.1 (ATP sulfurylase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 929.1 bits (2400), Expect = 1.6e-266
Identity = 457/484 (94.42%), Postives = 469/484 (96.90%), Query Frame = 0

Query: 1   MSLVTKLHIATSPQISLNTLQTSIKRT--PIITLNTNIKSKPIYVSEPLVFKPAMQGFSP 60
           MSLV KLH+ATSPQISLNTLQT+IKRT  P+ T  T IKSKPIYVSE L FKPAMQG+SP
Sbjct: 1   MSLVAKLHLATSPQISLNTLQTTIKRTPVPVATYYTKIKSKPIYVSESLGFKPAMQGYSP 60

Query: 61  RSVRSSIKSSLIEPDGGAVVELVVPESERASRVLEAEPLPKVKLTQIDLEWVHVICEGWA 120
           RS+RSSIK SLIEPDGGAVVEL+VPESERASRVLEAE LPKVKLT+IDLEWVHVI EGWA
Sbjct: 61  RSLRSSIKCSLIEPDGGAVVELIVPESERASRVLEAESLPKVKLTRIDLEWVHVISEGWA 120

Query: 121 SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPNG 180
           SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAI DDVKEQIGSS NVGLLGPNG
Sbjct: 121 SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIGDDVKEQIGSSPNVGLLGPNG 180

Query: 181 DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYND 240
           DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYND
Sbjct: 181 DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYND 240

Query: 241 GLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL 300
           GLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL
Sbjct: 241 GLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL 300

Query: 301 HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI 360
           HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI
Sbjct: 301 HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI 360

Query: 361 NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKK 420
           NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAK+
Sbjct: 361 NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKE 420

Query: 421 MAFFEPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEDASPQQP 480
           MAFF+PSRAKDFLFISGTKMRT+ARTGENPPDGFMCPGGWKVLVNYY+SLQAEDA+ QQP
Sbjct: 421 MAFFDPSRAKDFLFISGTKMRTFARTGENPPDGFMCPGGWKVLVNYYKSLQAEDATSQQP 480

Query: 481 ALSV 483
           ALSV
Sbjct: 481 ALSV 484

BLAST of Spg007221 vs. NCBI nr
Match: KAG6608587.1 (ATP sulfurylase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 927.9 bits (2397), Expect = 3.5e-266
Identity = 457/484 (94.42%), Postives = 468/484 (96.69%), Query Frame = 0

Query: 1   MSLVTKLHIATSPQISLNTLQTSIKRT--PIITLNTNIKSKPIYVSEPLVFKPAMQGFSP 60
           MSLV KLH+ATSPQISLNT QT+IKRT  PI T  T IKSKPIYVSE L FKPAMQG+SP
Sbjct: 1   MSLVAKLHLATSPQISLNTRQTTIKRTPVPIATYYTKIKSKPIYVSESLGFKPAMQGYSP 60

Query: 61  RSVRSSIKSSLIEPDGGAVVELVVPESERASRVLEAEPLPKVKLTQIDLEWVHVICEGWA 120
           RS+RSSIK SLIEPDGGAVVEL+VPESERASRVLEAE LPKVKLT+IDLEWVHVI EGWA
Sbjct: 61  RSLRSSIKCSLIEPDGGAVVELIVPESERASRVLEAESLPKVKLTRIDLEWVHVISEGWA 120

Query: 121 SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPNG 180
           SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAI DDVKEQIGSS NVGLLGPNG
Sbjct: 121 SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIGDDVKEQIGSSPNVGLLGPNG 180

Query: 181 DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYND 240
           DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYND
Sbjct: 181 DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYND 240

Query: 241 GLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL 300
           GLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL
Sbjct: 241 GLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL 300

Query: 301 HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI 360
           HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI
Sbjct: 301 HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI 360

Query: 361 NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKK 420
           NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDT+AK+
Sbjct: 361 NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTIAKE 420

Query: 421 MAFFEPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEDASPQQP 480
           MAFF+PSRAKDFLFISGTKMRT+ARTGENPPDGFMCPGGWKVLVNYY+SLQAEDAS QQP
Sbjct: 421 MAFFDPSRAKDFLFISGTKMRTFARTGENPPDGFMCPGGWKVLVNYYKSLQAEDASSQQP 480

Query: 481 ALSV 483
           ALSV
Sbjct: 481 ALSV 484

BLAST of Spg007221 vs. NCBI nr
Match: XP_022980900.1 (ATP sulfurylase 2 [Cucurbita maxima])

HSP 1 Score: 926.4 bits (2393), Expect = 1.0e-265
Identity = 456/484 (94.21%), Postives = 468/484 (96.69%), Query Frame = 0

Query: 1   MSLVTKLHIATSPQISLNTLQTSIKRT--PIITLNTNIKSKPIYVSEPLVFKPAMQGFSP 60
           MSLV KLH+ATSPQISLNTLQT+IKRT  PI T  T IKSKPIYVSE L F+PAMQG+SP
Sbjct: 1   MSLVAKLHLATSPQISLNTLQTTIKRTPVPIATYYTKIKSKPIYVSESLGFRPAMQGYSP 60

Query: 61  RSVRSSIKSSLIEPDGGAVVELVVPESERASRVLEAEPLPKVKLTQIDLEWVHVICEGWA 120
           RS+RSSIK SLIEPDGGAVVEL+VPESERASRVLEAE LPKVKLT+IDLEWVHVI EGWA
Sbjct: 61  RSLRSSIKCSLIEPDGGAVVELIVPESERASRVLEAESLPKVKLTRIDLEWVHVISEGWA 120

Query: 121 SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPNG 180
           SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAI DDVKEQIGSS NVGLLGPNG
Sbjct: 121 SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIGDDVKEQIGSSPNVGLLGPNG 180

Query: 181 DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYND 240
           DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYND
Sbjct: 181 DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYND 240

Query: 241 GLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL 300
           GLDHYRLSPKQLRQEFDRR+ADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL
Sbjct: 241 GLDHYRLSPKQLRQEFDRREADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL 300

Query: 301 HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI 360
           HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI
Sbjct: 301 HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI 360

Query: 361 NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKK 420
           NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDT+AK+
Sbjct: 361 NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTMAKE 420

Query: 421 MAFFEPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEDASPQQP 480
           MAFF PSRAKDFLFISGTKMRT+ARTGENPPDGFMCPGGWKVLVNYY+SLQAEDAS QQP
Sbjct: 421 MAFFNPSRAKDFLFISGTKMRTFARTGENPPDGFMCPGGWKVLVNYYKSLQAEDASSQQP 480

Query: 481 ALSV 483
           ALSV
Sbjct: 481 ALSV 484

BLAST of Spg007221 vs. NCBI nr
Match: XP_022941299.1 (ATP sulfurylase 2 [Cucurbita moschata])

HSP 1 Score: 926.0 bits (2392), Expect = 1.3e-265
Identity = 456/484 (94.21%), Postives = 467/484 (96.49%), Query Frame = 0

Query: 1   MSLVTKLHIATSPQISLNTLQTSIKRT--PIITLNTNIKSKPIYVSEPLVFKPAMQGFSP 60
           MSLV KLH+ATSPQISLNT QT+IKRT  PI    T IKSKPIYVSE L FKPAMQG+SP
Sbjct: 1   MSLVAKLHLATSPQISLNTRQTTIKRTPVPIAAYYTKIKSKPIYVSESLGFKPAMQGYSP 60

Query: 61  RSVRSSIKSSLIEPDGGAVVELVVPESERASRVLEAEPLPKVKLTQIDLEWVHVICEGWA 120
           RS+RSSIK SLIEPDGGAVVEL+VPESERASRVLEAE LPKVKLT+IDLEWVHVI EGWA
Sbjct: 61  RSLRSSIKCSLIEPDGGAVVELIVPESERASRVLEAESLPKVKLTRIDLEWVHVISEGWA 120

Query: 121 SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPNG 180
           SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAI DDVKEQIGSS NVGLLGPNG
Sbjct: 121 SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIGDDVKEQIGSSPNVGLLGPNG 180

Query: 181 DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYND 240
           DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDE+ITNAGNWLIGGDLEVINPIKYND
Sbjct: 181 DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEIITNAGNWLIGGDLEVINPIKYND 240

Query: 241 GLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL 300
           GLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL
Sbjct: 241 GLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL 300

Query: 301 HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI 360
           HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI
Sbjct: 301 HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI 360

Query: 361 NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKK 420
           NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAK+
Sbjct: 361 NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKE 420

Query: 421 MAFFEPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEDASPQQP 480
           MAFF+PSRAKDFLFISGTKMRT+ARTGENPPDGFMCPGGWKVLVNYY+SLQAEDAS QQP
Sbjct: 421 MAFFDPSRAKDFLFISGTKMRTFARTGENPPDGFMCPGGWKVLVNYYKSLQAEDASSQQP 480

Query: 481 ALSV 483
           ALSV
Sbjct: 481 ALSV 484

BLAST of Spg007221 vs. NCBI nr
Match: XP_038899984.1 (ATP sulfurylase 2 [Benincasa hispida])

HSP 1 Score: 915.2 bits (2364), Expect = 2.3e-262
Identity = 448/483 (92.75%), Postives = 465/483 (96.27%), Query Frame = 0

Query: 1   MSLVTKLHIATSPQISL-NTLQTSIKRTPIITLNTNIKSKPIYVSEPLVFKPAMQGFSPR 60
           MSLVTKLHIA SP+ ++ N LQT IK T I T N+ I+SKPIYVS PL+F+P MQG SPR
Sbjct: 1   MSLVTKLHIAISPKFTINNNLQTIIKTTSIPTYNSKIQSKPIYVSHPLLFEPKMQGHSPR 60

Query: 61  SVRSSIKSSLIEPDGGAVVELVVPESERASRVLEAEPLPKVKLTQIDLEWVHVICEGWAS 120
           S+RSSIKSSLI+PDGGA+V+LVVPE+ERASRVLEAEPLPKVKLT+IDLEWVHVI EGWAS
Sbjct: 61  SLRSSIKSSLIDPDGGALVDLVVPENERASRVLEAEPLPKVKLTRIDLEWVHVISEGWAS 120

Query: 121 PLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPNGD 180
           PL+GFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAI DDVK+QIGSSSNVGLLGP GD
Sbjct: 121 PLRGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIADDVKDQIGSSSNVGLLGPTGD 180

Query: 181 LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYNDG 240
           LVGILRSIEIFKHNKEER+ARTWGTT PGLPYVDEVITNAGNWLIGGDLEVINPIKYNDG
Sbjct: 181 LVGILRSIEIFKHNKEERMARTWGTTVPGLPYVDEVITNAGNWLIGGDLEVINPIKYNDG 240

Query: 241 LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH 300
           LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH
Sbjct: 241 LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH 300

Query: 301 PLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARIN 360
           PLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARIN
Sbjct: 301 PLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARIN 360

Query: 361 AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM 420
           AGANFYIVGRDPAGMGHP EKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM
Sbjct: 361 AGANFYIVGRDPAGMGHPKEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM 420

Query: 421 AFFEPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEDASPQQPA 480
           AFF+PSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEDASPQQPA
Sbjct: 421 AFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEDASPQQPA 480

Query: 481 LSV 483
           LSV
Sbjct: 481 LSV 483

BLAST of Spg007221 vs. ExPASy Swiss-Prot
Match: Q43870 (ATP sulfurylase 2 OS=Arabidopsis thaliana OX=3702 GN=APS2 PE=1 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 1.5e-216
Identity = 351/415 (84.58%), Postives = 384/415 (92.53%), Query Frame = 0

Query: 62  RSSIKSSLIEPDGGAVVELVVPESERASRVLEAEPLPKVKLTQIDLEWVHVICEGWASPL 121
           + ++KSSLI+PDGG +VEL+VPE+E   +  E+E +PKVKL QIDLEWVHVI EGWASPL
Sbjct: 57  KMTVKSSLIDPDGGELVELIVPETEIGVKKAESETMPKVKLNQIDLEWVHVISEGWASPL 116

Query: 122 KGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPNGDLV 181
           KGFMRE+EYLQSLHFN L++K+G+ VNMSLPIVLAIDDD KEQIGSS NV L+ P GD++
Sbjct: 117 KGFMREDEYLQSLHFNSLRLKNGTFVNMSLPIVLAIDDDTKEQIGSSENVALVCPQGDII 176

Query: 182 GILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYNDGLD 241
           G LRS+EI+KHNKEERIARTWGTT+PGLPYV+E IT +GNWLIGGDLEV  PIKYNDGLD
Sbjct: 177 GSLRSVEIYKHNKEERIARTWGTTSPGLPYVEEYITPSGNWLIGGDLEVFEPIKYNDGLD 236

Query: 242 HYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPL 301
           HYRLSPKQLR+EFD RQADAVFAFQLRNPVHNGHALLMNDTRKRLL+MGYKNP+LLLHPL
Sbjct: 237 HYRLSPKQLREEFDNRQADAVFAFQLRNPVHNGHALLMNDTRKRLLEMGYKNPVLLLHPL 296

Query: 302 GGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARINAG 361
           GGFTKADDVPLDVRMEQHSKVLEDGVLDP+TTIVSIFPSPMHYAGPTEVQWHAKARINAG
Sbjct: 297 GGFTKADDVPLDVRMEQHSKVLEDGVLDPKTTIVSIFPSPMHYAGPTEVQWHAKARINAG 356

Query: 362 ANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKMAF 421
           ANFYIVGRDPAGMGHPTEKRDLYDPDHGK+VLSMAPGLEKLNILPF+VAAYDT+ KKMAF
Sbjct: 357 ANFYIVGRDPAGMGHPTEKRDLYDPDHGKRVLSMAPGLEKLNILPFRVAAYDTIEKKMAF 416

Query: 422 FEPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEDASPQ 477
           F+PSRAK+FLFISGTKMRTYARTGENPPDGFMCP GW VLV YYESLQ  +A  Q
Sbjct: 417 FDPSRAKEFLFISGTKMRTYARTGENPPDGFMCPSGWNVLVKYYESLQESEAKQQ 471

BLAST of Spg007221 vs. ExPASy Swiss-Prot
Match: Q9LIK9 (ATP sulfurylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS1 PE=1 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 2.2e-194
Identity = 326/439 (74.26%), Postives = 370/439 (84.28%), Query Frame = 0

Query: 34  TNIKSKPIYVSEPLVFKP-AMQGFSPRSVR---SSIKSSLIEPDGGAVVELVVPESERAS 93
           T   S+P+  S P    P A   F  +S+R    SI++ LI PDGG +VEL+V E +R  
Sbjct: 11  TPFLSQPLTKSSPNSDLPFAAVSFPSKSLRRRVGSIRAGLIAPDGGKLVELIVEEPKRRE 70

Query: 94  RVLEAEPLPKVKLTQIDLEWVHVICEGWASPLKGFMREEEYLQSLHFNCLKIKDGSLVNM 153
           +  EA  LP+V+LT IDL+W+HV+ EGWASPL GFMRE E+LQ+LHFN L++ DGS+VNM
Sbjct: 71  KKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTLHFNSLRLDDGSVVNM 130

Query: 154 SLPIVLAIDDDVKEQIGSSSNVGLLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGL 213
           S+PIVLAIDD+ K +IG S+ V L   +G+ V IL  IEI+KH KEERIARTWGTTAPGL
Sbjct: 131 SVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIEIYKHPKEERIARTWGTTAPGL 190

Query: 214 PYVDEVITNAGNWLIGGDLEVINPIKYNDGLDHYRLSPKQLRQEFDRRQADAVFAFQLRN 273
           PYVDE ITNAGNWLIGGDLEV+ P+KYNDGLD +RLSP +LR+E ++R ADAVFAFQLRN
Sbjct: 191 PYVDEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSPAELRKELEKRNADAVFAFQLRN 250

Query: 274 PVHNGHALLMNDTRKRLLDMGYKNPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLD 333
           PVHNGHALLM DTR+RLL+MGYKNPILLLHPLGGFTKADDVPLD RM+QH KVLEDGVLD
Sbjct: 251 PVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLDWRMKQHEKVLEDGVLD 310

Query: 334 PETTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHG 393
           PETT+VSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP EKRDLYD DHG
Sbjct: 311 PETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPVEKRDLYDADHG 370

Query: 394 KKVLSMAPGLEKLNILPFKVAAYDTVAKKMAFFEPSRAKDFLFISGTKMRTYARTGENPP 453
           KKVLSMAPGLE+LNILPF+VAAYD    KMAFF+PSR +DFLFISGTKMRT A+  ENPP
Sbjct: 371 KKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTKMRTLAKNNENPP 430

Query: 454 DGFMCPGGWKVLVNYYESL 469
           DGFMCPGGWKVLV+YYESL
Sbjct: 431 DGFMCPGGWKVLVDYYESL 449

BLAST of Spg007221 vs. ExPASy Swiss-Prot
Match: O23324 (ATP-sulfurylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS3 PE=1 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 9.4e-190
Identity = 313/411 (76.16%), Postives = 357/411 (86.86%), Query Frame = 0

Query: 59  RSVRS-SIKSSLIEPDGGAVVELVVPESERASRVLEAEPLPKVKLTQIDLEWVHVICEGW 118
           RS+R+ S+++ LIEPDGG +V+LVVPE  R  +  EA  LP+V+LT IDL+W+HV+ EGW
Sbjct: 41  RSLRTISVRAGLIEPDGGKLVDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEGW 100

Query: 119 ASPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPN 178
           ASPL+GFMRE E+LQ+LHFN L + DGS+VNMS+PIVLAIDD  K  IG S  V L+  +
Sbjct: 101 ASPLRGFMRESEFLQTLHFNLLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSD 160

Query: 179 GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYN 238
            + + IL  IEI+KH KEERIARTWGTTAPGLPYV+E ITNAG+WLIGGDLEV+ P+KYN
Sbjct: 161 DNPIAILNDIEIYKHPKEERIARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYN 220

Query: 239 DGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL 298
           DGLD +RLSP +LR+E ++R ADAVFAFQLRNPVHNGHALLM DTR+RLL+MGYKNPILL
Sbjct: 221 DGLDRFRLSPFELRKELEKRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILL 280

Query: 299 LHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKAR 358
           LHPLGGFTKADDVPL  RM+QH KVLEDGVLDPETT+VSIFPSPM YAGPTEVQWHAKAR
Sbjct: 281 LHPLGGFTKADDVPLSWRMKQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKAR 340

Query: 359 INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAK 418
           INAGANFYIVGRDPAGMGHP EKRDLYD DHGKKVLSMAPGLE+LNILPF+VAAYD    
Sbjct: 341 INAGANFYIVGRDPAGMGHPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQG 400

Query: 419 KMAFFEPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESL 469
           KMAFF+PSRA+DFLFISGTKMR  A+  ENPPDGFMCPGGWKVLV+YY+SL
Sbjct: 401 KMAFFDPSRAQDFLFISGTKMRALAKNRENPPDGFMCPGGWKVLVDYYDSL 451

BLAST of Spg007221 vs. ExPASy Swiss-Prot
Match: Q9S7D8 (ATP sulfurylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS4 PE=2 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 6.7e-188
Identity = 313/409 (76.53%), Postives = 353/409 (86.31%), Query Frame = 0

Query: 64  SIKSSLIEPDGGAVVELVVPESERASRVLEAEPLP-KVKLTQIDLEWVHVICEGWASPLK 123
           S+KS LIEPDGG ++ LVV ES R     EAE +P ++KL ++DLEWVHV+ EGWASPLK
Sbjct: 48  SVKSGLIEPDGGKLMNLVVEESRRRVMKHEAETVPARIKLNRVDLEWVHVLSEGWASPLK 107

Query: 124 GFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPNGDLVG 183
           GFMR+ E+LQ+LHFN  +++DGS+VNMS+PIVLAIDDD K +IG S+ V L+   G+ + 
Sbjct: 108 GFMRQSEFLQTLHFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIA 167

Query: 184 ILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYNDGLDH 243
           IL  IEI+KH KEERIARTWGTTA GLPY +E IT AGNWLIGGDL+V+ PIKYNDGLD 
Sbjct: 168 ILNDIEIYKHPKEERIARTWGTTARGLPYAEEAITKAGNWLIGGDLQVLEPIKYNDGLDR 227

Query: 244 YRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPLG 303
           +RLSP QLR+EF RR ADAVFAFQLRNPVHNGHALLM DTR+RLL+MGYKNP+LLL+PLG
Sbjct: 228 FRLSPSQLREEFIRRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLNPLG 287

Query: 304 GFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARINAGA 363
           GFTKADDVPL  RM QH KVLEDGVLDPETT+VSIFPSPM YAGPTEVQWHAKARINAGA
Sbjct: 288 GFTKADDVPLSWRMRQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGA 347

Query: 364 NFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKMAFF 423
           NFYIVGRDPAGMGHPTEKRDLYD DHGKKVLSMAPGLE+LNILPFKVAAYD    KMAFF
Sbjct: 348 NFYIVGRDPAGMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFF 407

Query: 424 EPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAE 472
           +PSR++DFLFISGTKMR  A+  ENPPDGFMCP GWKVLV+YY+SL AE
Sbjct: 408 DPSRSQDFLFISGTKMRGLAKKKENPPDGFMCPSGWKVLVDYYDSLSAE 456

BLAST of Spg007221 vs. ExPASy Swiss-Prot
Match: O95340 (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 OS=Homo sapiens OX=9606 GN=PAPSS2 PE=1 SV=2)

HSP 1 Score: 501.1 bits (1289), Expect = 1.4e-140
Identity = 235/391 (60.10%), Postives = 301/391 (76.98%), Query Frame = 0

Query: 79  ELVVPESERASRVLEAEPLPKVKLTQIDLEWVHVICEGWASPLKGFMREEEYLQSLHFNC 138
           EL VPE++      EAE LP + +T++DL+WV V+ EGWA+PLKGFMRE+EYLQ +HF+ 
Sbjct: 226 ELFVPENKLDHVRAEAETLPSLSITKLDLQWVQVLSEGWATPLKGFMREKEYLQVMHFDT 285

Query: 139 LKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPNGDLVGILRSIEIFKHNKEERI 198
           L   D  ++NMS+PIVL +  + K ++   S   +L   G  V ILR  E ++H KEER 
Sbjct: 286 L--LDDGVINMSIPIVLPVSAEDKTRLEGCSKF-VLAHGGRRVAILRDAEFYEHRKEERC 345

Query: 199 ARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYNDGLDHYRLSPKQLRQEFDRRQ 258
           +R WGTT    P++ +++  +G+WL+GGDL+V+  I++NDGLD YRL+P +L+Q+     
Sbjct: 346 SRVWGTTCTKHPHI-KMVMESGDWLVGGDLQVLEKIRWNDGLDQYRLTPLELKQKCKEMN 405

Query: 259 ADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPLGGFTKADDVPLDVRMEQ 318
           ADAVFAFQLRNPVHNGHALLM DTR+RLL+ GYK+P+LLLHPLGG+TK DDVPLD RM+Q
Sbjct: 406 ADAVFAFQLRNPVHNGHALLMQDTRRRLLERGYKHPVLLLHPLGGWTKDDDVPLDWRMKQ 465

Query: 319 HSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPT 378
           H+ VLE+GVLDP++TIV+IFPSPM YAGPTEVQWH ++R+ AGANFYIVGRDPAGM HP 
Sbjct: 466 HAAVLEEGVLDPKSTIVAIFPSPMLYAGPTEVQWHCRSRMIAGANFYIVGRDPAGMPHPE 525

Query: 379 EKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKMAFFEPSRAKDFLFISGTKM 438
            K+DLY+P HG KVLSMAPGL  + I+PF+VAAY+   K M F++P+R  +F FISGT+M
Sbjct: 526 TKKDLYEPTHGGKVLSMAPGLTSVEIIPFRVAAYNKAKKAMDFYDPARHNEFDFISGTRM 585

Query: 439 RTYARTGENPPDGFMCPGGWKVLVNYYESLQ 470
           R  AR GENPPDGFM P  WKVL +YY SL+
Sbjct: 586 RKLAREGENPPDGFMAPKAWKVLTDYYRSLE 612

BLAST of Spg007221 vs. ExPASy TrEMBL
Match: A0A6J1IXV9 (Sulfate adenylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480215 PE=4 SV=1)

HSP 1 Score: 926.4 bits (2393), Expect = 4.9e-266
Identity = 456/484 (94.21%), Postives = 468/484 (96.69%), Query Frame = 0

Query: 1   MSLVTKLHIATSPQISLNTLQTSIKRT--PIITLNTNIKSKPIYVSEPLVFKPAMQGFSP 60
           MSLV KLH+ATSPQISLNTLQT+IKRT  PI T  T IKSKPIYVSE L F+PAMQG+SP
Sbjct: 1   MSLVAKLHLATSPQISLNTLQTTIKRTPVPIATYYTKIKSKPIYVSESLGFRPAMQGYSP 60

Query: 61  RSVRSSIKSSLIEPDGGAVVELVVPESERASRVLEAEPLPKVKLTQIDLEWVHVICEGWA 120
           RS+RSSIK SLIEPDGGAVVEL+VPESERASRVLEAE LPKVKLT+IDLEWVHVI EGWA
Sbjct: 61  RSLRSSIKCSLIEPDGGAVVELIVPESERASRVLEAESLPKVKLTRIDLEWVHVISEGWA 120

Query: 121 SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPNG 180
           SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAI DDVKEQIGSS NVGLLGPNG
Sbjct: 121 SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIGDDVKEQIGSSPNVGLLGPNG 180

Query: 181 DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYND 240
           DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYND
Sbjct: 181 DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYND 240

Query: 241 GLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL 300
           GLDHYRLSPKQLRQEFDRR+ADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL
Sbjct: 241 GLDHYRLSPKQLRQEFDRREADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL 300

Query: 301 HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI 360
           HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI
Sbjct: 301 HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI 360

Query: 361 NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKK 420
           NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDT+AK+
Sbjct: 361 NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTMAKE 420

Query: 421 MAFFEPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEDASPQQP 480
           MAFF PSRAKDFLFISGTKMRT+ARTGENPPDGFMCPGGWKVLVNYY+SLQAEDAS QQP
Sbjct: 421 MAFFNPSRAKDFLFISGTKMRTFARTGENPPDGFMCPGGWKVLVNYYKSLQAEDASSQQP 480

Query: 481 ALSV 483
           ALSV
Sbjct: 481 ALSV 484

BLAST of Spg007221 vs. ExPASy TrEMBL
Match: A0A6J1FMY2 (Sulfate adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446642 PE=4 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 6.4e-266
Identity = 456/484 (94.21%), Postives = 467/484 (96.49%), Query Frame = 0

Query: 1   MSLVTKLHIATSPQISLNTLQTSIKRT--PIITLNTNIKSKPIYVSEPLVFKPAMQGFSP 60
           MSLV KLH+ATSPQISLNT QT+IKRT  PI    T IKSKPIYVSE L FKPAMQG+SP
Sbjct: 1   MSLVAKLHLATSPQISLNTRQTTIKRTPVPIAAYYTKIKSKPIYVSESLGFKPAMQGYSP 60

Query: 61  RSVRSSIKSSLIEPDGGAVVELVVPESERASRVLEAEPLPKVKLTQIDLEWVHVICEGWA 120
           RS+RSSIK SLIEPDGGAVVEL+VPESERASRVLEAE LPKVKLT+IDLEWVHVI EGWA
Sbjct: 61  RSLRSSIKCSLIEPDGGAVVELIVPESERASRVLEAESLPKVKLTRIDLEWVHVISEGWA 120

Query: 121 SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPNG 180
           SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAI DDVKEQIGSS NVGLLGPNG
Sbjct: 121 SPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIGDDVKEQIGSSPNVGLLGPNG 180

Query: 181 DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYND 240
           DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDE+ITNAGNWLIGGDLEVINPIKYND
Sbjct: 181 DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEIITNAGNWLIGGDLEVINPIKYND 240

Query: 241 GLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL 300
           GLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL
Sbjct: 241 GLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL 300

Query: 301 HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI 360
           HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI
Sbjct: 301 HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI 360

Query: 361 NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKK 420
           NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAK+
Sbjct: 361 NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKE 420

Query: 421 MAFFEPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEDASPQQP 480
           MAFF+PSRAKDFLFISGTKMRT+ARTGENPPDGFMCPGGWKVLVNYY+SLQAEDAS QQP
Sbjct: 421 MAFFDPSRAKDFLFISGTKMRTFARTGENPPDGFMCPGGWKVLVNYYKSLQAEDASSQQP 480

Query: 481 ALSV 483
           ALSV
Sbjct: 481 ALSV 484

BLAST of Spg007221 vs. ExPASy TrEMBL
Match: A0A1S3CRF3 (Sulfate adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103503887 PE=4 SV=1)

HSP 1 Score: 903.7 bits (2334), Expect = 3.4e-259
Identity = 442/483 (91.51%), Postives = 460/483 (95.24%), Query Frame = 0

Query: 1   MSLVTKLHIATSPQISLNTLQTSIKRTPIITLNTNIKSKPIYVSEPLV-FKPAMQGFSPR 60
           MSL TKLHIA SPQ ++N LQT+ + T I   N+ I+SK IYVS PLV FK  MQG SPR
Sbjct: 1   MSLFTKLHIAISPQFTINNLQTTKRTTSIPIYNSKIQSKHIYVSHPLVLFKSTMQGHSPR 60

Query: 61  SVRSSIKSSLIEPDGGAVVELVVPESERASRVLEAEPLPKVKLTQIDLEWVHVICEGWAS 120
           S+RSSIKSSLI+PDGG +V+LVVPESERASR+LEAEPLPKVKLTQIDLEWVHVI EGWAS
Sbjct: 61  SLRSSIKSSLIDPDGGVIVDLVVPESERASRILEAEPLPKVKLTQIDLEWVHVISEGWAS 120

Query: 121 PLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPNGD 180
           PL+GFMRE+EYLQSLHFNC+KIKDGSLVNMSLPIVLAI DDVK+QIGSSSNVGLLGPNGD
Sbjct: 121 PLRGFMREDEYLQSLHFNCIKIKDGSLVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGD 180

Query: 181 LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYNDG 240
           LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVD VITNAGNWL+GGDLEVINPIKYNDG
Sbjct: 181 LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDVVITNAGNWLLGGDLEVINPIKYNDG 240

Query: 241 LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH 300
           LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH
Sbjct: 241 LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH 300

Query: 301 PLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARIN 360
           PLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARIN
Sbjct: 301 PLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARIN 360

Query: 361 AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM 420
           AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM
Sbjct: 361 AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM 420

Query: 421 AFFEPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEDASPQQPA 480
           AFF+PSRAKDFLFISGTKMR YARTGENPPDGFMCPGGWKVLVNYYESLQAE+ASPQQ A
Sbjct: 421 AFFDPSRAKDFLFISGTKMRAYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASPQQAA 480

Query: 481 LSV 483
           LSV
Sbjct: 481 LSV 483

BLAST of Spg007221 vs. ExPASy TrEMBL
Match: A0A0A0LE71 (Sulfate adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G872050 PE=4 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 5.8e-259
Identity = 442/483 (91.51%), Postives = 459/483 (95.03%), Query Frame = 0

Query: 1   MSLVTKLHIATSPQISLNTLQTSIKRTPIITLNTNIKSKPIYVSEPLV-FKPAMQGFSPR 60
           MSL TKLHIA SPQ ++N LQT+ K T I   N+ I+SK IYVS PLV FK  MQ  SPR
Sbjct: 1   MSLFTKLHIAISPQFTINNLQTTKKTTSIPIYNSKIQSKHIYVSHPLVLFKSTMQSHSPR 60

Query: 61  SVRSSIKSSLIEPDGGAVVELVVPESERASRVLEAEPLPKVKLTQIDLEWVHVICEGWAS 120
           S+RSSIK SLI+PDGG VV+LVVPESERASRVLEAEPLPKVKLTQIDLEWVHVI EGWAS
Sbjct: 61  SLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGWAS 120

Query: 121 PLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPNGD 180
           PL+GFMRE+EYLQSLHFNC+KIKDGS VNMSLPIVLAI DDVK+QIGSSSNVGLLGPNGD
Sbjct: 121 PLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGD 180

Query: 181 LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYNDG 240
           LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWL+GGDLEVINPIKYNDG
Sbjct: 181 LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDG 240

Query: 241 LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH 300
           LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH
Sbjct: 241 LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH 300

Query: 301 PLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARIN 360
           PLGGFTKADDVPLDVRMEQHSKVLEDG+LDPETTIVSIFPSPMHYAGPTEVQWHAKARIN
Sbjct: 301 PLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQWHAKARIN 360

Query: 361 AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM 420
           AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM
Sbjct: 361 AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM 420

Query: 421 AFFEPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEDASPQQPA 480
           AFF+P+RAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAE+ASPQQ A
Sbjct: 421 AFFDPTRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASPQQAA 480

Query: 481 LSV 483
           LSV
Sbjct: 481 LSV 483

BLAST of Spg007221 vs. ExPASy TrEMBL
Match: A0A6J1BZ55 (Sulfate adenylyltransferase OS=Momordica charantia OX=3673 GN=LOC111006556 PE=4 SV=1)

HSP 1 Score: 889.4 bits (2297), Expect = 6.6e-255
Identity = 433/482 (89.83%), Postives = 455/482 (94.40%), Query Frame = 0

Query: 1   MSLVTKLHIATSPQISLNTLQTSIKRTPIITLNTNIKSKPIYVSEPLVFKPAMQGFSPRS 60
           MSL+TKLHIATSPQ SLN LQ + K T I   +  IKSK IY+S PLVFK  MQ  SPRS
Sbjct: 1   MSLLTKLHIATSPQFSLN-LQPATKTTAITNHSAKIKSKTIYISRPLVFKLPMQASSPRS 60

Query: 61  VRSSIKSSLIEPDGGAVVELVVPESERASRVLEAEPLPKVKLTQIDLEWVHVICEGWASP 120
           +RSS+KSSLI+PDGGAVV+LVVP+ ERA+RVLEA+PLPKVKL++IDLEWVHVI EGWASP
Sbjct: 61  LRSSVKSSLIDPDGGAVVDLVVPDGERAARVLEADPLPKVKLSKIDLEWVHVISEGWASP 120

Query: 121 LKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPNGDL 180
           L+GFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAI DDVK+QIGSS NVGLLGP+GDL
Sbjct: 121 LRGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIADDVKDQIGSSPNVGLLGPSGDL 180

Query: 181 VGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYNDGL 240
           VGILRS+EIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYNDGL
Sbjct: 181 VGILRSVEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYNDGL 240

Query: 241 DHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHP 300
           DH+RLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHP
Sbjct: 241 DHFRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHP 300

Query: 301 LGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARINA 360
           LGGFTKADDVPLDVRMEQHSK LEDGVLDPETTIV+IFPSPMHYAGPTEVQWHAKARINA
Sbjct: 301 LGGFTKADDVPLDVRMEQHSKALEDGVLDPETTIVAIFPSPMHYAGPTEVQWHAKARINA 360

Query: 361 GANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKMA 420
           GANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPF+VAAYDTVAKKMA
Sbjct: 361 GANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFRVAAYDTVAKKMA 420

Query: 421 FFEPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEDASPQQPAL 480
           FFEP RAKDFLFISGTKMR +ARTGENPPDGFMCP GWKVLVNYY+SLQ EDA PQQPAL
Sbjct: 421 FFEPGRAKDFLFISGTKMRNFARTGENPPDGFMCPSGWKVLVNYYQSLQVEDAPPQQPAL 480

Query: 481 SV 483
           SV
Sbjct: 481 SV 481

BLAST of Spg007221 vs. TAIR 10
Match: AT1G19920.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 753.4 bits (1944), Expect = 1.1e-217
Identity = 351/415 (84.58%), Postives = 384/415 (92.53%), Query Frame = 0

Query: 62  RSSIKSSLIEPDGGAVVELVVPESERASRVLEAEPLPKVKLTQIDLEWVHVICEGWASPL 121
           + ++KSSLI+PDGG +VEL+VPE+E   +  E+E +PKVKL QIDLEWVHVI EGWASPL
Sbjct: 57  KMTVKSSLIDPDGGELVELIVPETEIGVKKAESETMPKVKLNQIDLEWVHVISEGWASPL 116

Query: 122 KGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPNGDLV 181
           KGFMRE+EYLQSLHFN L++K+G+ VNMSLPIVLAIDDD KEQIGSS NV L+ P GD++
Sbjct: 117 KGFMREDEYLQSLHFNSLRLKNGTFVNMSLPIVLAIDDDTKEQIGSSENVALVCPQGDII 176

Query: 182 GILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYNDGLD 241
           G LRS+EI+KHNKEERIARTWGTT+PGLPYV+E IT +GNWLIGGDLEV  PIKYNDGLD
Sbjct: 177 GSLRSVEIYKHNKEERIARTWGTTSPGLPYVEEYITPSGNWLIGGDLEVFEPIKYNDGLD 236

Query: 242 HYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPL 301
           HYRLSPKQLR+EFD RQADAVFAFQLRNPVHNGHALLMNDTRKRLL+MGYKNP+LLLHPL
Sbjct: 237 HYRLSPKQLREEFDNRQADAVFAFQLRNPVHNGHALLMNDTRKRLLEMGYKNPVLLLHPL 296

Query: 302 GGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARINAG 361
           GGFTKADDVPLDVRMEQHSKVLEDGVLDP+TTIVSIFPSPMHYAGPTEVQWHAKARINAG
Sbjct: 297 GGFTKADDVPLDVRMEQHSKVLEDGVLDPKTTIVSIFPSPMHYAGPTEVQWHAKARINAG 356

Query: 362 ANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKMAF 421
           ANFYIVGRDPAGMGHPTEKRDLYDPDHGK+VLSMAPGLEKLNILPF+VAAYDT+ KKMAF
Sbjct: 357 ANFYIVGRDPAGMGHPTEKRDLYDPDHGKRVLSMAPGLEKLNILPFRVAAYDTIEKKMAF 416

Query: 422 FEPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEDASPQ 477
           F+PSRAK+FLFISGTKMRTYARTGENPPDGFMCP GW VLV YYESLQ  +A  Q
Sbjct: 417 FDPSRAKEFLFISGTKMRTYARTGENPPDGFMCPSGWNVLVKYYESLQESEAKQQ 471

BLAST of Spg007221 vs. TAIR 10
Match: AT3G22890.1 (ATP sulfurylase 1 )

HSP 1 Score: 679.9 bits (1753), Expect = 1.5e-195
Identity = 326/439 (74.26%), Postives = 370/439 (84.28%), Query Frame = 0

Query: 34  TNIKSKPIYVSEPLVFKP-AMQGFSPRSVR---SSIKSSLIEPDGGAVVELVVPESERAS 93
           T   S+P+  S P    P A   F  +S+R    SI++ LI PDGG +VEL+V E +R  
Sbjct: 11  TPFLSQPLTKSSPNSDLPFAAVSFPSKSLRRRVGSIRAGLIAPDGGKLVELIVEEPKRRE 70

Query: 94  RVLEAEPLPKVKLTQIDLEWVHVICEGWASPLKGFMREEEYLQSLHFNCLKIKDGSLVNM 153
           +  EA  LP+V+LT IDL+W+HV+ EGWASPL GFMRE E+LQ+LHFN L++ DGS+VNM
Sbjct: 71  KKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTLHFNSLRLDDGSVVNM 130

Query: 154 SLPIVLAIDDDVKEQIGSSSNVGLLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGL 213
           S+PIVLAIDD+ K +IG S+ V L   +G+ V IL  IEI+KH KEERIARTWGTTAPGL
Sbjct: 131 SVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIEIYKHPKEERIARTWGTTAPGL 190

Query: 214 PYVDEVITNAGNWLIGGDLEVINPIKYNDGLDHYRLSPKQLRQEFDRRQADAVFAFQLRN 273
           PYVDE ITNAGNWLIGGDLEV+ P+KYNDGLD +RLSP +LR+E ++R ADAVFAFQLRN
Sbjct: 191 PYVDEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSPAELRKELEKRNADAVFAFQLRN 250

Query: 274 PVHNGHALLMNDTRKRLLDMGYKNPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLD 333
           PVHNGHALLM DTR+RLL+MGYKNPILLLHPLGGFTKADDVPLD RM+QH KVLEDGVLD
Sbjct: 251 PVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLDWRMKQHEKVLEDGVLD 310

Query: 334 PETTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHG 393
           PETT+VSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP EKRDLYD DHG
Sbjct: 311 PETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPVEKRDLYDADHG 370

Query: 394 KKVLSMAPGLEKLNILPFKVAAYDTVAKKMAFFEPSRAKDFLFISGTKMRTYARTGENPP 453
           KKVLSMAPGLE+LNILPF+VAAYD    KMAFF+PSR +DFLFISGTKMRT A+  ENPP
Sbjct: 371 KKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTKMRTLAKNNENPP 430

Query: 454 DGFMCPGGWKVLVNYYESL 469
           DGFMCPGGWKVLV+YYESL
Sbjct: 431 DGFMCPGGWKVLVDYYESL 449

BLAST of Spg007221 vs. TAIR 10
Match: AT4G14680.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 664.5 bits (1713), Expect = 6.7e-191
Identity = 313/411 (76.16%), Postives = 357/411 (86.86%), Query Frame = 0

Query: 59  RSVRS-SIKSSLIEPDGGAVVELVVPESERASRVLEAEPLPKVKLTQIDLEWVHVICEGW 118
           RS+R+ S+++ LIEPDGG +V+LVVPE  R  +  EA  LP+V+LT IDL+W+HV+ EGW
Sbjct: 41  RSLRTISVRAGLIEPDGGKLVDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEGW 100

Query: 119 ASPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPN 178
           ASPL+GFMRE E+LQ+LHFN L + DGS+VNMS+PIVLAIDD  K  IG S  V L+  +
Sbjct: 101 ASPLRGFMRESEFLQTLHFNLLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSD 160

Query: 179 GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYN 238
            + + IL  IEI+KH KEERIARTWGTTAPGLPYV+E ITNAG+WLIGGDLEV+ P+KYN
Sbjct: 161 DNPIAILNDIEIYKHPKEERIARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYN 220

Query: 239 DGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL 298
           DGLD +RLSP +LR+E ++R ADAVFAFQLRNPVHNGHALLM DTR+RLL+MGYKNPILL
Sbjct: 221 DGLDRFRLSPFELRKELEKRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILL 280

Query: 299 LHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKAR 358
           LHPLGGFTKADDVPL  RM+QH KVLEDGVLDPETT+VSIFPSPM YAGPTEVQWHAKAR
Sbjct: 281 LHPLGGFTKADDVPLSWRMKQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKAR 340

Query: 359 INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAK 418
           INAGANFYIVGRDPAGMGHP EKRDLYD DHGKKVLSMAPGLE+LNILPF+VAAYD    
Sbjct: 341 INAGANFYIVGRDPAGMGHPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQG 400

Query: 419 KMAFFEPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESL 469
           KMAFF+PSRA+DFLFISGTKMR  A+  ENPPDGFMCPGGWKVLV+YY+SL
Sbjct: 401 KMAFFDPSRAQDFLFISGTKMRALAKNRENPPDGFMCPGGWKVLVDYYDSL 451

BLAST of Spg007221 vs. TAIR 10
Match: AT5G43780.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 658.3 bits (1697), Expect = 4.8e-189
Identity = 313/409 (76.53%), Postives = 353/409 (86.31%), Query Frame = 0

Query: 64  SIKSSLIEPDGGAVVELVVPESERASRVLEAEPLP-KVKLTQIDLEWVHVICEGWASPLK 123
           S+KS LIEPDGG ++ LVV ES R     EAE +P ++KL ++DLEWVHV+ EGWASPLK
Sbjct: 48  SVKSGLIEPDGGKLMNLVVEESRRRVMKHEAETVPARIKLNRVDLEWVHVLSEGWASPLK 107

Query: 124 GFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIDDDVKEQIGSSSNVGLLGPNGDLVG 183
           GFMR+ E+LQ+LHFN  +++DGS+VNMS+PIVLAIDDD K +IG S+ V L+   G+ + 
Sbjct: 108 GFMRQSEFLQTLHFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIA 167

Query: 184 ILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYNDGLDH 243
           IL  IEI+KH KEERIARTWGTTA GLPY +E IT AGNWLIGGDL+V+ PIKYNDGLD 
Sbjct: 168 ILNDIEIYKHPKEERIARTWGTTARGLPYAEEAITKAGNWLIGGDLQVLEPIKYNDGLDR 227

Query: 244 YRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPLG 303
           +RLSP QLR+EF RR ADAVFAFQLRNPVHNGHALLM DTR+RLL+MGYKNP+LLL+PLG
Sbjct: 228 FRLSPSQLREEFIRRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLNPLG 287

Query: 304 GFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARINAGA 363
           GFTKADDVPL  RM QH KVLEDGVLDPETT+VSIFPSPM YAGPTEVQWHAKARINAGA
Sbjct: 288 GFTKADDVPLSWRMRQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGA 347

Query: 364 NFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKMAFF 423
           NFYIVGRDPAGMGHPTEKRDLYD DHGKKVLSMAPGLE+LNILPFKVAAYD    KMAFF
Sbjct: 348 NFYIVGRDPAGMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFF 407

Query: 424 EPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAE 472
           +PSR++DFLFISGTKMR  A+  ENPPDGFMCP GWKVLV+YY+SL AE
Sbjct: 408 DPSRSQDFLFISGTKMRGLAKKKENPPDGFMCPSGWKVLVDYYDSLSAE 456

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023525367.11.6e-26694.42ATP sulfurylase 2 [Cucurbita pepo subsp. pepo][more]
KAG6608587.13.5e-26694.42ATP sulfurylase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022980900.11.0e-26594.21ATP sulfurylase 2 [Cucurbita maxima][more]
XP_022941299.11.3e-26594.21ATP sulfurylase 2 [Cucurbita moschata][more]
XP_038899984.12.3e-26292.75ATP sulfurylase 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q438701.5e-21684.58ATP sulfurylase 2 OS=Arabidopsis thaliana OX=3702 GN=APS2 PE=1 SV=1[more]
Q9LIK92.2e-19474.26ATP sulfurylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS1 PE=1 SV... [more]
O233249.4e-19076.16ATP-sulfurylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS3 PE=1 SV... [more]
Q9S7D86.7e-18876.53ATP sulfurylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS4 PE=2 SV... [more]
O953401.4e-14060.10Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 OS=Homo sapiens OX... [more]
Match NameE-valueIdentityDescription
A0A6J1IXV94.9e-26694.21Sulfate adenylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480215 PE=4 SV=... [more]
A0A6J1FMY26.4e-26694.21Sulfate adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446642 PE=4 S... [more]
A0A1S3CRF33.4e-25991.51Sulfate adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103503887 PE=4 SV=1[more]
A0A0A0LE715.8e-25991.51Sulfate adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G872050 PE=4 SV=1[more]
A0A6J1BZ556.6e-25589.83Sulfate adenylyltransferase OS=Momordica charantia OX=3673 GN=LOC111006556 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT1G19920.11.1e-21784.58Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
AT3G22890.11.5e-19574.26ATP sulfurylase 1 [more]
AT4G14680.16.7e-19176.16Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
AT5G43780.14.8e-18976.53Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025980ATP-sulfurylase PUA-like domainPFAMPF14306PUA_2coord: 70..232
e-value: 9.0E-45
score: 152.2
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 237..470
e-value: 1.2E-94
score: 317.9
NoneNo IPR availableGENE3D3.10.400.10Sulfate adenylyltransferasecoord: 68..236
e-value: 1.9E-57
score: 195.4
NoneNo IPR availablePANTHERPTHR11055:SF51ENDOGLUCANASEcoord: 51..476
NoneNo IPR availablePANTHERPTHR11055BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASEcoord: 51..476
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 242..465
IPR002650Sulphate adenylyltransferaseTIGRFAMTIGR00339TIGR00339coord: 72..462
e-value: 8.1E-129
score: 427.7
IPR002650Sulphate adenylyltransferaseCDDcd00517ATPScoord: 97..464
e-value: 4.14992E-169
score: 478.666
IPR024951Sulphate adenylyltransferase catalytic domainPFAMPF01747ATP-sulfurylasecoord: 242..463
e-value: 1.3E-66
score: 224.2
IPR015947PUA-like superfamilySUPERFAMILY88697PUA domain-likecoord: 70..238

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg007221.1Spg007221.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009970 cellular response to sulfate starvation
biological_process GO:0016310 phosphorylation
biological_process GO:0000103 sulfate assimilation
cellular_component GO:0009507 chloroplast
molecular_function GO:0004020 adenylylsulfate kinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004781 sulfate adenylyltransferase (ATP) activity