Spg006156 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg006156
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionSNF1-related protein kinase regulatory subunit beta-2-like isoform X1
Locationscaffold4: 4827269 .. 4831032 (-)
RNA-Seq ExpressionSpg006156
SyntenySpg006156
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGCTCTGGAGATGTCTCTTTTGAAGATCTACATCGAGCACATTGCTGTCAGGATGGGCCAACGAAAGAAGAAGCAGACTCATGTAGATGCTCCACCGAGTGGCGGTGTCAGCATCTCCAACTTGGTGATGTCAACATTTAACCACCAAACTCTCAACTCCATACGCAAAAGGAGGGCAGTGGAAGTTTACGATGTACGTGCTAACTCCTAACATGGCCTGCATTTATTTTGGGTTTTCTCATCCCTCTCCATCTATATCTCTGTCTCTCTCTACCTATCCATTTTTTCTTTTCTTTTTTCACTCTGGTTCTGCTCTGGTGGTTGATTGTTGAGAGAGAAAAATCACTTTCATTAGATTCTTGCACAGCCTTTTTCCCCAAGGACCAAATGATTGCAAAAGTTTAAGCCTAGAATTGGTATCATTTCCCCTCAACTTTTTACTGTTTATCGTTGATTGGATGGACAAAAGAACGAGATGCTACTTTACCTTTTCTTTTTGATACTTCTGCACTCCTTAGTCTAGTCCCTCTGGCTCTGGCCATTGGGGTTATTTTGAAGTTATTTTTGTAGTATATATATATAAATATTATATTATCACATCAAATAGAGAGGACTTCACTCACTTTTTTCTAATAAGAGGATCCACTCTTGAATAAAGTTTGCTTAATCGAGCATACCTTCAACTTTAGTCTCTCTCAAGGTATTAAGTGATTTGGACAACACTAATCGAGTTATCTCAATGTTTTGATCTGCTGTCTGTAACCTTACAGCACATTTGAGCATAGATCCATATGCTCACTTGCTGTGAGAAGGGGTTTCCTTGAATGATTTGGAAACAAGTCACATGGGGAGGCAGTTTTACTTTAGGAATGTCTTTCCATGGGGGGGGGGAGAGAGAGAGAGCTTGGGCTTGCAAAAGCAGCGCTTTTGCTGCTTTCAGCTACTTGGACTTCCACCCAGATTGAGTATTTGACTTTCGTACAACAACAGAGACAAGTATGCTTAGGCGACATCTTCTTCCCTAAGCAAACAAGTTAGAGGTCAGGTCAAATGTCATATAGTATTTTTCTTACAAATGTTTGTAAGATGAATTCTACAATTTCCGTTTAGAACGCTTCACAAATATGCACAAGAAGTTGTTATCCGAAAGCAACATCCAGTTCTAATCAACAGCTCATGCTTGTTTGTTCTAAGCGGGAGTTTTCTGTCTTTTCAAGCGACTTTTGGCCATTTTCTAACCTAATCTCCGGCCTTTGTGTATCAATTGTTTTTATAAAATTATGAGTTACCCGTGAGCATGAATCTGAGATCCCAATAGCCAAGATAGGTAAAACAGTAATTTTAAACCATGATTTGGAGCCCATCTCAATGCAGTGGCAGTTGAGAGTGGCTGTTGGAGGCTCCAAAAGACTCCCTGCAGACGAAGGAAAAAAAGTAATATTGGTGATGACGAGGCAACATTCTCTTTTAAATCAGCATAATTACCAACCCACTAAAAGAATCTTCCAAATTCATTGGAGAATAAAAAACCACTGTTTATCGTTCAGTTGGATTTTTTTAATCACAGCAGCAAGAGGGATTTGGGCTGAAAAGAAGAGGAAAAGAAGGGAGAGAAAGTGTCCTTGGGAAGGAAGAGAGAGAGAGAGAGAGAGGACAAATTGCACTTTCCAAAATAAAACTTAACCGAACTCCATAAATACACAGCTGCAGCATGCGCAAGGAAGATGCTAACAGAATCCAACCTTCACCCAACTCTTGGTTTGGATTCTAAATTTTTGTGGGATTGATCAACTGGCGTAAGTATGTGTAGAAATCGTCTGCTTTTGTGGGTTTTTGTTGTTTGCCTGTACCCCTTTTCTTTTCGATTGGCCAATTTTTTGAGTTAGAATTCTGATTTGGATTGTCGTCTGTTATTTAAATCCTGGTTTTCGTTCTGTTTTTCAGTGATGGACAATGCTGAGGATAGGAAAAATGGAGAAGGCCCCTCTGGAGCCCATAAGTTTCAGGAGAATCATGACCAAAGGATGGACTGTGCAGACGATGCAGCACATAACAGCTACCACACTTACACATTTTCAGAGTCCAGAATTCAGATTTCTCCACAAAGCTTAGGCTCACCTCAAGCCCTCATCTCTAATCCTCAGGTTTGTTCTTCCGTATCTTTCTTTGTTTTTTAAGTTCATAATTATGCACTCTCTCTCTCTCTCACCCACTTCCTTGATTCTTTGAACCGAGGAATATTTCTGGACCTGTTTAAATGGGGGCATTTTCTTTTTAAAACTACGGGAATTTGTTCTAAGCATCCTGATATGTTAAATAATCGAATATGCAGATTATTGTCTCTCTTTAAGCTTTTTTTGTTAATGATTTGGTTACATTACAGACGACGGGTCGTTCTTTGTAACTGCTCTCTTTTTCTGTGAATTGTTGCATATATTCTTCTCCTTCACTCCAGTTGAAGGCGACTTGCTAAAAGGGTTCTGCTTTCTATATTTTGTGTTAGGAAAATCAGTGCCTTTAACTTCATCCTGAAAATTGTAATTCATGGTCAGTTAGCTACATGTAGCAAATTGCTCTTCTAATGTTTCTTTCTTTATAGAAAAGGAAAACTCTTCATTTTCTTTTTATGATTAATATTAGGTGCAATTCAAGCTGTAAAGACGATCGTTTATCCTTTTTTATTATTTGTTGTCTGAAAGTACAGGAGAAAATTGAGCACCTAAAATTGGCATTAATTGCCTAATAACTATGCCAAAAGAGGGTGTCTACACTGAGTGGGACCTTGTATTTATAAGAACAAATCCGCCAAAAATATTGTACCTACTTTGGTTTATTGAACCACCGATAACACTCAAATTCACCCTCTCATTGTCTTGTTTTCATTCATCTTCACTTCTAGTACGCTACAGTTTCCTTGTCAAGTCCTGGTCTGATGCTACAACCTCAACCTCAATCTTTGCCTCAGAGTGCTGCATTCAGTGAAAATGTGTTCCATGAGACAAGGACGCGAGTGAGGATCTCGTGGAATCATGGCGGCAATCAAGTGGCCATTGTTGGATCATGGGACAACTGGCAGACCAGGTATTCGACTTCGATGATGTGCGTTTTTCTTTCTCATTCCTTTTCATTTTCTAAACTCAAACAACCTTTAATTGTGTCCTGTATTTCTGTTAGAGAGCTCCTGCAGAACACAGGTGAGAAGTTTGTGGTTATAAAGACTCTGCCAGTCGGTATCTACCACTACCACTTCATAGTTGATGGTTGGTTGGTTTATGCTCCAGATCTACCCTGGTTTTGTGATGATTCAGGGAATGCATTCAATATTTTAGACTTGCAGGTAAATTTCATGGCTTCCCATTGAGAATTTTGTATTCCTCAAGAATGCTATACTGCTGCTCCACATAATTCTCATTGAACTATGATTTCCAGGGCCATGTTTCTGAATTGCCTGAAAGTATGTCTGAGTTTGAAGCTCCTCCATCCCCGCGGTCGAGTTATGACAATCAATACTTGAACGAAGATGATTTCAGCAGACCTCCACCAGAACTACCTCCACATCTGCAAGGAACCATTTTGAACGATCCATCTTCTTCAATCGATGGTCGACAGTTGCAAGTTACGCCTCGAAGTACAGAATTAAATCATCTGTATCTACAGAGCAATGTCCAAGATCAGTTTCTAGCACTTGGCTCTACTCACAGGATTCGTGAAAAGCATGTTACAATGTTTGTATTCAAGCCTTTATCAAGAACAAGGTGA

mRNA sequence

ATGGCAGCTCTGGAGATGTCTCTTTTGAAGATCTACATCGAGCACATTGCTGTCAGGATGGGCCAACGAAAGAAGAAGCAGACTCATGTAGATGCTCCACCGAGTGGCGGTGTCAGCATCTCCAACTTGGTGATGTCAACATTTAACCACCAAACTCTCAACTCCATACGCAAAAGGAGGGCAGTGGAAGTTTACGATAATTCTGATTTGGATTGTCGTCTGTTATTTAAATCCTGGTTTTCGTTCTGTTTTTCAGTGATGGACAATGCTGAGGATAGGAAAAATGGAGAAGGCCCCTCTGGAGCCCATAAGTTTCAGGAGAATCATGACCAAAGGATGGACTGTGCAGACGATGCAGCACATAACAGCTACCACACTTACACATTTTCAGAGTCCAGAATTCAGATTTCTCCACAAAGCTTAGGCTCACCTCAAGCCCTCATCTCTAATCCTCAGTACGCTACAGTTTCCTTGTCAAGTCCTGGTCTGATGCTACAACCTCAACCTCAATCTTTGCCTCAGAGTGCTGCATTCAGTGAAAATGTGTTCCATGAGACAAGGACGCGAGTGAGGATCTCGTGGAATCATGGCGGCAATCAAGTGGCCATTGTTGGATCATGGGACAACTGGCAGACCAGAGAGCTCCTGCAGAACACAGGTGAGAAGTTTGTGGTTATAAAGACTCTGCCAGTCGGTATCTACCACTACCACTTCATAGTTGATGGTTGGTTGGTTTATGCTCCAGATCTACCCTGGTTTTGTGATGATTCAGGGAATGCATTCAATATTTTAGACTTGCAGGGCCATGTTTCTGAATTGCCTGAAAGTATGTCTGAGTTTGAAGCTCCTCCATCCCCGCGGTCGAGTTATGACAATCAATACTTGAACGAAGATGATTTCAGCAGACCTCCACCAGAACTACCTCCACATCTGCAAGGAACCATTTTGAACGATCCATCTTCTTCAATCGATGGTCGACAGTTGCAAGTTACGCCTCGAAGTACAGAATTAAATCATCTGTATCTACAGAGCAATGTCCAAGATCAGTTTCTAGCACTTGGCTCTACTCACAGGATTCGTGAAAAGCATGTTACAATGTTTGTATTCAAGCCTTTATCAAGAACAAGGTGA

Coding sequence (CDS)

ATGGCAGCTCTGGAGATGTCTCTTTTGAAGATCTACATCGAGCACATTGCTGTCAGGATGGGCCAACGAAAGAAGAAGCAGACTCATGTAGATGCTCCACCGAGTGGCGGTGTCAGCATCTCCAACTTGGTGATGTCAACATTTAACCACCAAACTCTCAACTCCATACGCAAAAGGAGGGCAGTGGAAGTTTACGATAATTCTGATTTGGATTGTCGTCTGTTATTTAAATCCTGGTTTTCGTTCTGTTTTTCAGTGATGGACAATGCTGAGGATAGGAAAAATGGAGAAGGCCCCTCTGGAGCCCATAAGTTTCAGGAGAATCATGACCAAAGGATGGACTGTGCAGACGATGCAGCACATAACAGCTACCACACTTACACATTTTCAGAGTCCAGAATTCAGATTTCTCCACAAAGCTTAGGCTCACCTCAAGCCCTCATCTCTAATCCTCAGTACGCTACAGTTTCCTTGTCAAGTCCTGGTCTGATGCTACAACCTCAACCTCAATCTTTGCCTCAGAGTGCTGCATTCAGTGAAAATGTGTTCCATGAGACAAGGACGCGAGTGAGGATCTCGTGGAATCATGGCGGCAATCAAGTGGCCATTGTTGGATCATGGGACAACTGGCAGACCAGAGAGCTCCTGCAGAACACAGGTGAGAAGTTTGTGGTTATAAAGACTCTGCCAGTCGGTATCTACCACTACCACTTCATAGTTGATGGTTGGTTGGTTTATGCTCCAGATCTACCCTGGTTTTGTGATGATTCAGGGAATGCATTCAATATTTTAGACTTGCAGGGCCATGTTTCTGAATTGCCTGAAAGTATGTCTGAGTTTGAAGCTCCTCCATCCCCGCGGTCGAGTTATGACAATCAATACTTGAACGAAGATGATTTCAGCAGACCTCCACCAGAACTACCTCCACATCTGCAAGGAACCATTTTGAACGATCCATCTTCTTCAATCGATGGTCGACAGTTGCAAGTTACGCCTCGAAGTACAGAATTAAATCATCTGTATCTACAGAGCAATGTCCAAGATCAGTTTCTAGCACTTGGCTCTACTCACAGGATTCGTGAAAAGCATGTTACAATGTTTGTATTCAAGCCTTTATCAAGAACAAGGTGA

Protein sequence

MAALEMSLLKIYIEHIAVRMGQRKKKQTHVDAPPSGGVSISNLVMSTFNHQTLNSIRKRRAVEVYDNSDLDCRLLFKSWFSFCFSVMDNAEDRKNGEGPSGAHKFQENHDQRMDCADDAAHNSYHTYTFSESRIQISPQSLGSPQALISNPQYATVSLSSPGLMLQPQPQSLPQSAAFSENVFHETRTRVRISWNHGGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFIVDGWLVYAPDLPWFCDDSGNAFNILDLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDDFSRPPPELPPHLQGTILNDPSSSIDGRQLQVTPRSTELNHLYLQSNVQDQFLALGSTHRIREKHVTMFVFKPLSRTR
Homology
BLAST of Spg006156 vs. NCBI nr
Match: XP_022928365.1 (SNF1-related protein kinase regulatory subunit beta-2-like isoform X7 [Cucurbita moschata])

HSP 1 Score: 566.6 bits (1459), Expect = 1.6e-157
Identity = 288/377 (76.39%), Postives = 312/377 (82.76%), Query Frame = 0

Query: 1   MAALEMSLLKIYIEHIAVRMGQRKKKQTHVDAPPSGGVSISNLVMSTFNHQTLNSIRKRR 60
           MAALEMSLLK+YIEHIAVRMGQ KKKQTHVDAPP GGVS SNLV+STFNH TLN     R
Sbjct: 1   MAALEMSLLKVYIEHIAVRMGQGKKKQTHVDAPPGGGVSFSNLVISTFNHLTLNFKEGSR 60

Query: 61  AVEVYDNSDLDCRLLFKSWFSFCFSVMDNAEDRKNGEGPSGAHKFQENHDQRMDCADDAA 120
           ++                       +MDNAE  KN +  SG HKF+ENHDQRMDC +DA 
Sbjct: 61  SLR----------------------LMDNAEGSKNRDDLSGVHKFEENHDQRMDCTEDAI 120

Query: 121 HNSYHTYTFSESRIQISPQSLGSPQALISNPQYATVSLSSPGLMLQPQPQSLPQS-AAFS 180
            NS+HT +F++SRIQISPQSLGS       PQYATVSLSSPGLMLQPQPQS+PQS AAF 
Sbjct: 121 QNSHHTRSFTDSRIQISPQSLGS-------PQYATVSLSSPGLMLQPQPQSVPQSAAAFI 180

Query: 181 ENVFHETRTRVRISWNHGGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFI 240
           ENVFHETR+RVRISWNHGGNQVAIVGSWDNWQ+RELLQNTGEKFVV+KTLPVGIYHYHFI
Sbjct: 181 ENVFHETRSRVRISWNHGGNQVAIVGSWDNWQSRELLQNTGEKFVVVKTLPVGIYHYHFI 240

Query: 241 VDGWLVYAPDLPWFCDDSGNAFNILDLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDD 300
           VDGWLVYAPDLPWF +DSGNA+NILDL G+VSELPESM EFE PPSP SSYDNQY NEDD
Sbjct: 241 VDGWLVYAPDLPWFRNDSGNAYNILDLPGYVSELPESMFEFETPPSPPSSYDNQYFNEDD 300

Query: 301 FSRPPPELPPHLQGTILNDPSSSIDGRQLQVTPRSTELNHLYLQSNVQDQFLALGSTHRI 360
           F RPPPELPP LQGTILNDPSSS+DGR L VTPR+TELNHLYLQ NVQDQFLALGS+ RI
Sbjct: 301 FCRPPPELPPQLQGTILNDPSSSVDGRPLPVTPRNTELNHLYLQRNVQDQFLALGSSQRI 348

Query: 361 REKHVTMFVFKPLSRTR 377
            EK+ TMF+FKPLSRTR
Sbjct: 361 HEKYYTMFLFKPLSRTR 348

BLAST of Spg006156 vs. NCBI nr
Match: XP_022928361.1 (SNF1-related protein kinase regulatory subunit beta-2-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 566.6 bits (1459), Expect = 1.6e-157
Identity = 288/377 (76.39%), Postives = 312/377 (82.76%), Query Frame = 0

Query: 1   MAALEMSLLKIYIEHIAVRMGQRKKKQTHVDAPPSGGVSISNLVMSTFNHQTLNSIRKRR 60
           MAALEMSLLK+YIEHIAVRMGQ KKKQTHVDAPP GGVS SNLV+STFNH TLN     R
Sbjct: 1   MAALEMSLLKVYIEHIAVRMGQGKKKQTHVDAPPGGGVSFSNLVISTFNHLTLNFKEGSR 60

Query: 61  AVEVYDNSDLDCRLLFKSWFSFCFSVMDNAEDRKNGEGPSGAHKFQENHDQRMDCADDAA 120
           ++                       +MDNAE  KN +  SG HKF+ENHDQRMDC +DA 
Sbjct: 61  SLR----------------------LMDNAEGSKNRDDLSGVHKFEENHDQRMDCTEDAI 120

Query: 121 HNSYHTYTFSESRIQISPQSLGSPQALISNPQYATVSLSSPGLMLQPQPQSLPQS-AAFS 180
            NS+HT +F++SRIQISPQSLGS       PQYATVSLSSPGLMLQPQPQS+PQS AAF 
Sbjct: 121 QNSHHTRSFTDSRIQISPQSLGS-------PQYATVSLSSPGLMLQPQPQSVPQSAAAFI 180

Query: 181 ENVFHETRTRVRISWNHGGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFI 240
           ENVFHETR+RVRISWNHGGNQVAIVGSWDNWQ+RELLQNTGEKFVV+KTLPVGIYHYHFI
Sbjct: 181 ENVFHETRSRVRISWNHGGNQVAIVGSWDNWQSRELLQNTGEKFVVVKTLPVGIYHYHFI 240

Query: 241 VDGWLVYAPDLPWFCDDSGNAFNILDLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDD 300
           VDGWLVYAPDLPWF +DSGNA+NILDL G+VSELPESM EFE PPSP SSYDNQY NEDD
Sbjct: 241 VDGWLVYAPDLPWFRNDSGNAYNILDLPGYVSELPESMFEFETPPSPPSSYDNQYFNEDD 300

Query: 301 FSRPPPELPPHLQGTILNDPSSSIDGRQLQVTPRSTELNHLYLQSNVQDQFLALGSTHRI 360
           F RPPPELPP LQGTILNDPSSS+DGR L VTPR+TELNHLYLQ NVQDQFLALGS+ RI
Sbjct: 301 FCRPPPELPPQLQGTILNDPSSSVDGRPLPVTPRNTELNHLYLQRNVQDQFLALGSSQRI 348

Query: 361 REKHVTMFVFKPLSRTR 377
            EK+ TMF+FKPLSRTR
Sbjct: 361 HEKYYTMFLFKPLSRTR 348

BLAST of Spg006156 vs. NCBI nr
Match: XP_022928364.1 (SNF1-related protein kinase regulatory subunit beta-2-like isoform X6 [Cucurbita moschata])

HSP 1 Score: 565.1 bits (1455), Expect = 4.6e-157
Identity = 287/376 (76.33%), Postives = 311/376 (82.71%), Query Frame = 0

Query: 1   MAALEMSLLKIYIEHIAVRMGQRKKKQTHVDAPPSGGVSISNLVMSTFNHQTLNSIRKRR 60
           MAALEMSLLK+YIEHIAVRMGQ KKKQTHVDAPP GGVS SNLV+STFNH TLN     R
Sbjct: 1   MAALEMSLLKVYIEHIAVRMGQGKKKQTHVDAPPGGGVSFSNLVISTFNHLTLNFKEGSR 60

Query: 61  AVEVYDNSDLDCRLLFKSWFSFCFSVMDNAEDRKNGEGPSGAHKFQENHDQRMDCADDAA 120
           ++                       +MDNAE  KN +  SG HKF+ENHDQRMDC +DA 
Sbjct: 61  SLR----------------------LMDNAEGSKNRDDLSGVHKFEENHDQRMDCTEDAI 120

Query: 121 HNSYHTYTFSESRIQISPQSLGSPQALISNPQYATVSLSSPGLMLQPQPQSLPQS-AAFS 180
            NS+HT +F++SRIQISPQSLGS       PQYATVSLSSPGLMLQPQPQS+PQS AAF 
Sbjct: 121 QNSHHTRSFTDSRIQISPQSLGS-------PQYATVSLSSPGLMLQPQPQSVPQSAAAFI 180

Query: 181 ENVFHETRTRVRISWNHGGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFI 240
           ENVFHETR+RVRISWNHGGNQVAIVGSWDNWQ+RELLQNTGEKFVV+KTLPVGIYHYHFI
Sbjct: 181 ENVFHETRSRVRISWNHGGNQVAIVGSWDNWQSRELLQNTGEKFVVVKTLPVGIYHYHFI 240

Query: 241 VDGWLVYAPDLPWFCDDSGNAFNILDLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDD 300
           VDGWLVYAPDLPWF +DSGNA+NILDL G+VSELPESM EFE PPSP SSYDNQY NEDD
Sbjct: 241 VDGWLVYAPDLPWFRNDSGNAYNILDLPGYVSELPESMFEFETPPSPPSSYDNQYFNEDD 300

Query: 301 FSRPPPELPPHLQGTILNDPSSSIDGRQLQVTPRSTELNHLYLQSNVQDQFLALGSTHRI 360
           F RPPPELPP LQGTILNDPSSS+DGR L VTPR+TELNHLYLQ NVQDQFLALGS+ RI
Sbjct: 301 FCRPPPELPPQLQGTILNDPSSSVDGRPLPVTPRNTELNHLYLQRNVQDQFLALGSSQRI 347

Query: 361 REKHVTMFVFKPLSRT 376
            EK+ TMF+FKPLSRT
Sbjct: 361 HEKYYTMFLFKPLSRT 347

BLAST of Spg006156 vs. NCBI nr
Match: XP_022928358.1 (SNF1-related protein kinase regulatory subunit beta-2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 565.1 bits (1455), Expect = 4.6e-157
Identity = 287/376 (76.33%), Postives = 311/376 (82.71%), Query Frame = 0

Query: 1   MAALEMSLLKIYIEHIAVRMGQRKKKQTHVDAPPSGGVSISNLVMSTFNHQTLNSIRKRR 60
           MAALEMSLLK+YIEHIAVRMGQ KKKQTHVDAPP GGVS SNLV+STFNH TLN     R
Sbjct: 1   MAALEMSLLKVYIEHIAVRMGQGKKKQTHVDAPPGGGVSFSNLVISTFNHLTLNFKEGSR 60

Query: 61  AVEVYDNSDLDCRLLFKSWFSFCFSVMDNAEDRKNGEGPSGAHKFQENHDQRMDCADDAA 120
           ++                       +MDNAE  KN +  SG HKF+ENHDQRMDC +DA 
Sbjct: 61  SLR----------------------LMDNAEGSKNRDDLSGVHKFEENHDQRMDCTEDAI 120

Query: 121 HNSYHTYTFSESRIQISPQSLGSPQALISNPQYATVSLSSPGLMLQPQPQSLPQS-AAFS 180
            NS+HT +F++SRIQISPQSLGS       PQYATVSLSSPGLMLQPQPQS+PQS AAF 
Sbjct: 121 QNSHHTRSFTDSRIQISPQSLGS-------PQYATVSLSSPGLMLQPQPQSVPQSAAAFI 180

Query: 181 ENVFHETRTRVRISWNHGGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFI 240
           ENVFHETR+RVRISWNHGGNQVAIVGSWDNWQ+RELLQNTGEKFVV+KTLPVGIYHYHFI
Sbjct: 181 ENVFHETRSRVRISWNHGGNQVAIVGSWDNWQSRELLQNTGEKFVVVKTLPVGIYHYHFI 240

Query: 241 VDGWLVYAPDLPWFCDDSGNAFNILDLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDD 300
           VDGWLVYAPDLPWF +DSGNA+NILDL G+VSELPESM EFE PPSP SSYDNQY NEDD
Sbjct: 241 VDGWLVYAPDLPWFRNDSGNAYNILDLPGYVSELPESMFEFETPPSPPSSYDNQYFNEDD 300

Query: 301 FSRPPPELPPHLQGTILNDPSSSIDGRQLQVTPRSTELNHLYLQSNVQDQFLALGSTHRI 360
           F RPPPELPP LQGTILNDPSSS+DGR L VTPR+TELNHLYLQ NVQDQFLALGS+ RI
Sbjct: 301 FCRPPPELPPQLQGTILNDPSSSVDGRPLPVTPRNTELNHLYLQRNVQDQFLALGSSQRI 347

Query: 361 REKHVTMFVFKPLSRT 376
            EK+ TMF+FKPLSRT
Sbjct: 361 HEKYYTMFLFKPLSRT 347

BLAST of Spg006156 vs. NCBI nr
Match: XP_022928360.1 (SNF1-related protein kinase regulatory subunit beta-2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 562.0 bits (1447), Expect = 3.9e-156
Identity = 285/374 (76.20%), Postives = 309/374 (82.62%), Query Frame = 0

Query: 4   LEMSLLKIYIEHIAVRMGQRKKKQTHVDAPPSGGVSISNLVMSTFNHQTLNSIRKRRAVE 63
           LEMSLLK+YIEHIAVRMGQ KKKQTHVDAPP GGVS SNLV+STFNH TLN     R++ 
Sbjct: 11  LEMSLLKVYIEHIAVRMGQGKKKQTHVDAPPGGGVSFSNLVISTFNHLTLNFKEGSRSLR 70

Query: 64  VYDNSDLDCRLLFKSWFSFCFSVMDNAEDRKNGEGPSGAHKFQENHDQRMDCADDAAHNS 123
                                 +MDNAE  KN +  SG HKF+ENHDQRMDC +DA  NS
Sbjct: 71  ----------------------LMDNAEGSKNRDDLSGVHKFEENHDQRMDCTEDAIQNS 130

Query: 124 YHTYTFSESRIQISPQSLGSPQALISNPQYATVSLSSPGLMLQPQPQSLPQS-AAFSENV 183
           +HT +F++SRIQISPQSLGS       PQYATVSLSSPGLMLQPQPQS+PQS AAF ENV
Sbjct: 131 HHTRSFTDSRIQISPQSLGS-------PQYATVSLSSPGLMLQPQPQSVPQSAAAFIENV 190

Query: 184 FHETRTRVRISWNHGGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFIVDG 243
           FHETR+RVRISWNHGGNQVAIVGSWDNWQ+RELLQNTGEKFVV+KTLPVGIYHYHFIVDG
Sbjct: 191 FHETRSRVRISWNHGGNQVAIVGSWDNWQSRELLQNTGEKFVVVKTLPVGIYHYHFIVDG 250

Query: 244 WLVYAPDLPWFCDDSGNAFNILDLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDDFSR 303
           WLVYAPDLPWF +DSGNA+NILDL G+VSELPESM EFE PPSP SSYDNQY NEDDF R
Sbjct: 251 WLVYAPDLPWFRNDSGNAYNILDLPGYVSELPESMFEFETPPSPPSSYDNQYFNEDDFCR 310

Query: 304 PPPELPPHLQGTILNDPSSSIDGRQLQVTPRSTELNHLYLQSNVQDQFLALGSTHRIREK 363
           PPPELPP LQGTILNDPSSS+DGR L VTPR+TELNHLYLQ NVQDQFLALGS+ RI EK
Sbjct: 311 PPPELPPQLQGTILNDPSSSVDGRPLPVTPRNTELNHLYLQRNVQDQFLALGSSQRIHEK 355

Query: 364 HVTMFVFKPLSRTR 377
           + TMF+FKPLSRTR
Sbjct: 371 YYTMFLFKPLSRTR 355

BLAST of Spg006156 vs. ExPASy Swiss-Prot
Match: Q9SCY5 (SNF1-related protein kinase regulatory subunit beta-2 OS=Arabidopsis thaliana OX=3702 GN=KINB2 PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 4.3e-49
Identity = 104/239 (43.51%), Postives = 145/239 (60.67%), Query Frame = 0

Query: 137 SPQSLGSPQALISNPQYATVSLSSPGLMLQPQPQSLPQSAAFSENVFHETRTRVRISWNH 196
           SP++  SP  L+  PQ   + L  P  +  P P  +   ++  E   +E      I+W H
Sbjct: 53  SPRATQSP--LMFAPQVPVLPLQRPDEIHIPNPSWMQSPSSLYEEASNEQGIPTMITWCH 112

Query: 197 GGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFIVDGWLVYAPDLPWFCDD 256
           GG ++A+ GSWDNW+TR  LQ +G+ F ++K LP G+Y Y FIVDG   +AP+LP   DD
Sbjct: 113 GGKEIAVEGSWDNWKTRSRLQRSGKDFTIMKVLPSGVYEYRFIVDGQWRHAPELPLARDD 172

Query: 257 SGNAFNILDLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDDFSRPPPELPPHLQGTIL 316
           +GN FNILDLQ +V E  +S+S FE P SP +SY N  L  +D+S+ PP +PPHLQ T+L
Sbjct: 173 AGNTFNILDLQDYVPEDIQSISGFEPPQSPENSYSNLLLGAEDYSKEPPVVPPHLQMTLL 232

Query: 317 NDPSSSIDGRQLQVTPRSTELNHLYLQSNVQ-DQFLALGSTHRIREKHVTMFVFKPLSR 375
           N P+++ D       P+   LNHLY+Q        +ALGSTHR   K+VT+ ++K L R
Sbjct: 233 NLPAANPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALGSTHRFLAKYVTVVLYKSLQR 289

BLAST of Spg006156 vs. ExPASy Swiss-Prot
Match: Q84VQ1 (SNF1-related protein kinase regulatory subunit beta-1 OS=Arabidopsis thaliana OX=3702 GN=KINB1 PE=1 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.0e-38
Identity = 105/291 (36.08%), Postives = 149/291 (51.20%), Query Frame = 0

Query: 89  NAEDRKNGEGPSGAHKFQENHDQRMDCADDAAHNSYHTYTFSESRIQISPQS-LGSPQAL 148
           N +D     G  GA     +   R +  D +A  S H    S+S     P S   SP   
Sbjct: 5   NGKDEDAAAGSGGAD--VTSSSARSNGGDPSA-RSRHRRPSSDSMSSSPPGSPARSPSPF 64

Query: 149 ISNPQYATVSLSSPGLMLQPQPQSLPQSAAFSENVFH---ETRTRVRISWNHGGNQVAIV 208
           +  PQ     L        P P ++  +   S+ VF    E      I+WN GGN VA+ 
Sbjct: 65  LFAPQVPVAPLQRAN---APPPNNIQWNQ--SQRVFDNPPEQGIPTIITWNQGGNDVAVE 124

Query: 209 GSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFIVDGWLVYAPDLPWFCDDSGNAFNIL 268
           GSWDNW++R+ LQ +G+   ++  LP GIYHY  IVDG   Y PDLP+  D+ GN  NIL
Sbjct: 125 GSWDNWRSRKKLQKSGKDHSILFVLPSGIYHYKVIVDGESKYIPDLPFVADEVGNVCNIL 184

Query: 269 DLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDDFSRPPPELPPHLQGTILNDPSSSID 328
           D+   V E PES+ EFEAPPSP  SY       +D+++ P  +PP L  T+L     + +
Sbjct: 185 DVHNFVPENPESIVEFEAPPSPDHSYGQTLPAAEDYAKEPLAVPPQLHLTLL----GTTE 244

Query: 329 GRQLQVTPRSTELNHLYLQSNVQDQ-FLALGSTHRIREKHVTMFVFKPLSR 375
              +   P+   LNH++++     Q  +ALG THR   K++T+ ++KPL+R
Sbjct: 245 ETAIATKPQHVVLNHVFIEQGWTPQSIVALGLTHRFESKYITVVLYKPLTR 283

BLAST of Spg006156 vs. ExPASy Swiss-Prot
Match: O43741 (5'-AMP-activated protein kinase subunit beta-2 OS=Homo sapiens OX=9606 GN=PRKAB2 PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 3.2e-20
Identity = 65/194 (33.51%), Postives = 96/194 (49.48%), Query Frame = 0

Query: 192 ISWNHGGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFIVDGWLVYAPDLP 251
           I W+ GG +V I GS++NW T+  L  +   FV I  LP G + Y F VDG  V+ P  P
Sbjct: 81  IRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEP 140

Query: 252 WFCDDSGNAFNILDLQGHVSEL-----PESMSEFE--------APPSPRSSYDNQYLNED 311
                 G   N++ ++    E+      +SM   E        +PP P       + +E+
Sbjct: 141 VVTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYAFRSEE 200

Query: 312 DFSRPPPELPPHLQGTILNDPSSSIDGRQLQVTPRSTELNHLYLQSNVQDQFLALGSTHR 371
            F + PP LPPHL   ILN  ++      L   P    LNHLY  S ++D  + L +THR
Sbjct: 201 RF-KSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALS-IKDSVMVLSATHR 260

Query: 372 IREKHVTMFVFKPL 373
            ++K+VT  ++KP+
Sbjct: 261 YKKKYVTTLLYKPI 272

BLAST of Spg006156 vs. ExPASy Swiss-Prot
Match: Q6PAM0 (5'-AMP-activated protein kinase subunit beta-2 OS=Mus musculus OX=10090 GN=Prkab2 PE=1 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 4.2e-20
Identity = 65/194 (33.51%), Postives = 96/194 (49.48%), Query Frame = 0

Query: 192 ISWNHGGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFIVDGWLVYAPDLP 251
           I W+ GG +V I GS++NW T+  L  +   FV I  LP G + Y F VDG  V+ P  P
Sbjct: 80  IRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEP 139

Query: 252 WFCDDSGNAFNILDLQGHVSEL-----PESMSEFE--------APPSPRSSYDNQYLNED 311
                 G   N++ ++    E+      +SM   E        +PP P       + +E+
Sbjct: 140 VVTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYVFRSEE 199

Query: 312 DFSRPPPELPPHLQGTILNDPSSSIDGRQLQVTPRSTELNHLYLQSNVQDQFLALGSTHR 371
            F + PP LPPHL   ILN  ++      L   P    LNHLY  S ++D  + L +THR
Sbjct: 200 RF-KSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALS-IKDSVMVLSATHR 259

Query: 372 IREKHVTMFVFKPL 373
            ++K+VT  ++KP+
Sbjct: 260 YKKKYVTTLLYKPI 271

BLAST of Spg006156 vs. ExPASy Swiss-Prot
Match: Q9QZH4 (5'-AMP-activated protein kinase subunit beta-2 OS=Rattus norvegicus OX=10116 GN=Prkab2 PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 9.4e-20
Identity = 65/194 (33.51%), Postives = 95/194 (48.97%), Query Frame = 0

Query: 192 ISWNHGGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFIVDGWLVYAPDLP 251
           I W+ GG +V I GS++NW T+  L  +   FV I  LP G + Y F VDG  V+ P  P
Sbjct: 80  IRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEP 139

Query: 252 WFCDDSGNAFNILDLQGHVSEL-----PESMSEFE--------APPSPRSSYDNQYLNED 311
                 G   N++ ++    E+      +SM   E        +PP P       + +E+
Sbjct: 140 VVTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYVFRSEE 199

Query: 312 DFSRPPPELPPHLQGTILNDPSSSIDGRQLQVTPRSTELNHLYLQSNVQDQFLALGSTHR 371
            F + PP LPPHL   ILN  ++      L   P    LNHLY  S  +D  + L +THR
Sbjct: 200 RF-KSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALS-TKDSVMVLSATHR 259

Query: 372 IREKHVTMFVFKPL 373
            ++K+VT  ++KP+
Sbjct: 260 YKKKYVTTLLYKPI 271

BLAST of Spg006156 vs. ExPASy TrEMBL
Match: A0A6J1ERI1 (SNF1-related protein kinase regulatory subunit beta-2-like isoform X7 OS=Cucurbita moschata OX=3662 GN=LOC111435212 PE=3 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 7.7e-158
Identity = 288/377 (76.39%), Postives = 312/377 (82.76%), Query Frame = 0

Query: 1   MAALEMSLLKIYIEHIAVRMGQRKKKQTHVDAPPSGGVSISNLVMSTFNHQTLNSIRKRR 60
           MAALEMSLLK+YIEHIAVRMGQ KKKQTHVDAPP GGVS SNLV+STFNH TLN     R
Sbjct: 1   MAALEMSLLKVYIEHIAVRMGQGKKKQTHVDAPPGGGVSFSNLVISTFNHLTLNFKEGSR 60

Query: 61  AVEVYDNSDLDCRLLFKSWFSFCFSVMDNAEDRKNGEGPSGAHKFQENHDQRMDCADDAA 120
           ++                       +MDNAE  KN +  SG HKF+ENHDQRMDC +DA 
Sbjct: 61  SLR----------------------LMDNAEGSKNRDDLSGVHKFEENHDQRMDCTEDAI 120

Query: 121 HNSYHTYTFSESRIQISPQSLGSPQALISNPQYATVSLSSPGLMLQPQPQSLPQS-AAFS 180
            NS+HT +F++SRIQISPQSLGS       PQYATVSLSSPGLMLQPQPQS+PQS AAF 
Sbjct: 121 QNSHHTRSFTDSRIQISPQSLGS-------PQYATVSLSSPGLMLQPQPQSVPQSAAAFI 180

Query: 181 ENVFHETRTRVRISWNHGGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFI 240
           ENVFHETR+RVRISWNHGGNQVAIVGSWDNWQ+RELLQNTGEKFVV+KTLPVGIYHYHFI
Sbjct: 181 ENVFHETRSRVRISWNHGGNQVAIVGSWDNWQSRELLQNTGEKFVVVKTLPVGIYHYHFI 240

Query: 241 VDGWLVYAPDLPWFCDDSGNAFNILDLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDD 300
           VDGWLVYAPDLPWF +DSGNA+NILDL G+VSELPESM EFE PPSP SSYDNQY NEDD
Sbjct: 241 VDGWLVYAPDLPWFRNDSGNAYNILDLPGYVSELPESMFEFETPPSPPSSYDNQYFNEDD 300

Query: 301 FSRPPPELPPHLQGTILNDPSSSIDGRQLQVTPRSTELNHLYLQSNVQDQFLALGSTHRI 360
           F RPPPELPP LQGTILNDPSSS+DGR L VTPR+TELNHLYLQ NVQDQFLALGS+ RI
Sbjct: 301 FCRPPPELPPQLQGTILNDPSSSVDGRPLPVTPRNTELNHLYLQRNVQDQFLALGSSQRI 348

Query: 361 REKHVTMFVFKPLSRTR 377
            EK+ TMF+FKPLSRTR
Sbjct: 361 HEKYYTMFLFKPLSRTR 348

BLAST of Spg006156 vs. ExPASy TrEMBL
Match: A0A6J1EK37 (SNF1-related protein kinase regulatory subunit beta-2-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111435212 PE=3 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 7.7e-158
Identity = 288/377 (76.39%), Postives = 312/377 (82.76%), Query Frame = 0

Query: 1   MAALEMSLLKIYIEHIAVRMGQRKKKQTHVDAPPSGGVSISNLVMSTFNHQTLNSIRKRR 60
           MAALEMSLLK+YIEHIAVRMGQ KKKQTHVDAPP GGVS SNLV+STFNH TLN     R
Sbjct: 1   MAALEMSLLKVYIEHIAVRMGQGKKKQTHVDAPPGGGVSFSNLVISTFNHLTLNFKEGSR 60

Query: 61  AVEVYDNSDLDCRLLFKSWFSFCFSVMDNAEDRKNGEGPSGAHKFQENHDQRMDCADDAA 120
           ++                       +MDNAE  KN +  SG HKF+ENHDQRMDC +DA 
Sbjct: 61  SLR----------------------LMDNAEGSKNRDDLSGVHKFEENHDQRMDCTEDAI 120

Query: 121 HNSYHTYTFSESRIQISPQSLGSPQALISNPQYATVSLSSPGLMLQPQPQSLPQS-AAFS 180
            NS+HT +F++SRIQISPQSLGS       PQYATVSLSSPGLMLQPQPQS+PQS AAF 
Sbjct: 121 QNSHHTRSFTDSRIQISPQSLGS-------PQYATVSLSSPGLMLQPQPQSVPQSAAAFI 180

Query: 181 ENVFHETRTRVRISWNHGGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFI 240
           ENVFHETR+RVRISWNHGGNQVAIVGSWDNWQ+RELLQNTGEKFVV+KTLPVGIYHYHFI
Sbjct: 181 ENVFHETRSRVRISWNHGGNQVAIVGSWDNWQSRELLQNTGEKFVVVKTLPVGIYHYHFI 240

Query: 241 VDGWLVYAPDLPWFCDDSGNAFNILDLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDD 300
           VDGWLVYAPDLPWF +DSGNA+NILDL G+VSELPESM EFE PPSP SSYDNQY NEDD
Sbjct: 241 VDGWLVYAPDLPWFRNDSGNAYNILDLPGYVSELPESMFEFETPPSPPSSYDNQYFNEDD 300

Query: 301 FSRPPPELPPHLQGTILNDPSSSIDGRQLQVTPRSTELNHLYLQSNVQDQFLALGSTHRI 360
           F RPPPELPP LQGTILNDPSSS+DGR L VTPR+TELNHLYLQ NVQDQFLALGS+ RI
Sbjct: 301 FCRPPPELPPQLQGTILNDPSSSVDGRPLPVTPRNTELNHLYLQRNVQDQFLALGSSQRI 348

Query: 361 REKHVTMFVFKPLSRTR 377
            EK+ TMF+FKPLSRTR
Sbjct: 361 HEKYYTMFLFKPLSRTR 348

BLAST of Spg006156 vs. ExPASy TrEMBL
Match: A0A6J1ENS9 (SNF1-related protein kinase regulatory subunit beta-2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435212 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.2e-157
Identity = 287/376 (76.33%), Postives = 311/376 (82.71%), Query Frame = 0

Query: 1   MAALEMSLLKIYIEHIAVRMGQRKKKQTHVDAPPSGGVSISNLVMSTFNHQTLNSIRKRR 60
           MAALEMSLLK+YIEHIAVRMGQ KKKQTHVDAPP GGVS SNLV+STFNH TLN     R
Sbjct: 1   MAALEMSLLKVYIEHIAVRMGQGKKKQTHVDAPPGGGVSFSNLVISTFNHLTLNFKEGSR 60

Query: 61  AVEVYDNSDLDCRLLFKSWFSFCFSVMDNAEDRKNGEGPSGAHKFQENHDQRMDCADDAA 120
           ++                       +MDNAE  KN +  SG HKF+ENHDQRMDC +DA 
Sbjct: 61  SLR----------------------LMDNAEGSKNRDDLSGVHKFEENHDQRMDCTEDAI 120

Query: 121 HNSYHTYTFSESRIQISPQSLGSPQALISNPQYATVSLSSPGLMLQPQPQSLPQS-AAFS 180
            NS+HT +F++SRIQISPQSLGS       PQYATVSLSSPGLMLQPQPQS+PQS AAF 
Sbjct: 121 QNSHHTRSFTDSRIQISPQSLGS-------PQYATVSLSSPGLMLQPQPQSVPQSAAAFI 180

Query: 181 ENVFHETRTRVRISWNHGGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFI 240
           ENVFHETR+RVRISWNHGGNQVAIVGSWDNWQ+RELLQNTGEKFVV+KTLPVGIYHYHFI
Sbjct: 181 ENVFHETRSRVRISWNHGGNQVAIVGSWDNWQSRELLQNTGEKFVVVKTLPVGIYHYHFI 240

Query: 241 VDGWLVYAPDLPWFCDDSGNAFNILDLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDD 300
           VDGWLVYAPDLPWF +DSGNA+NILDL G+VSELPESM EFE PPSP SSYDNQY NEDD
Sbjct: 241 VDGWLVYAPDLPWFRNDSGNAYNILDLPGYVSELPESMFEFETPPSPPSSYDNQYFNEDD 300

Query: 301 FSRPPPELPPHLQGTILNDPSSSIDGRQLQVTPRSTELNHLYLQSNVQDQFLALGSTHRI 360
           F RPPPELPP LQGTILNDPSSS+DGR L VTPR+TELNHLYLQ NVQDQFLALGS+ RI
Sbjct: 301 FCRPPPELPPQLQGTILNDPSSSVDGRPLPVTPRNTELNHLYLQRNVQDQFLALGSSQRI 347

Query: 361 REKHVTMFVFKPLSRT 376
            EK+ TMF+FKPLSRT
Sbjct: 361 HEKYYTMFLFKPLSRT 347

BLAST of Spg006156 vs. ExPASy TrEMBL
Match: A0A6J1EKM3 (SNF1-related protein kinase regulatory subunit beta-2-like isoform X6 OS=Cucurbita moschata OX=3662 GN=LOC111435212 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.2e-157
Identity = 287/376 (76.33%), Postives = 311/376 (82.71%), Query Frame = 0

Query: 1   MAALEMSLLKIYIEHIAVRMGQRKKKQTHVDAPPSGGVSISNLVMSTFNHQTLNSIRKRR 60
           MAALEMSLLK+YIEHIAVRMGQ KKKQTHVDAPP GGVS SNLV+STFNH TLN     R
Sbjct: 1   MAALEMSLLKVYIEHIAVRMGQGKKKQTHVDAPPGGGVSFSNLVISTFNHLTLNFKEGSR 60

Query: 61  AVEVYDNSDLDCRLLFKSWFSFCFSVMDNAEDRKNGEGPSGAHKFQENHDQRMDCADDAA 120
           ++                       +MDNAE  KN +  SG HKF+ENHDQRMDC +DA 
Sbjct: 61  SLR----------------------LMDNAEGSKNRDDLSGVHKFEENHDQRMDCTEDAI 120

Query: 121 HNSYHTYTFSESRIQISPQSLGSPQALISNPQYATVSLSSPGLMLQPQPQSLPQS-AAFS 180
            NS+HT +F++SRIQISPQSLGS       PQYATVSLSSPGLMLQPQPQS+PQS AAF 
Sbjct: 121 QNSHHTRSFTDSRIQISPQSLGS-------PQYATVSLSSPGLMLQPQPQSVPQSAAAFI 180

Query: 181 ENVFHETRTRVRISWNHGGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFI 240
           ENVFHETR+RVRISWNHGGNQVAIVGSWDNWQ+RELLQNTGEKFVV+KTLPVGIYHYHFI
Sbjct: 181 ENVFHETRSRVRISWNHGGNQVAIVGSWDNWQSRELLQNTGEKFVVVKTLPVGIYHYHFI 240

Query: 241 VDGWLVYAPDLPWFCDDSGNAFNILDLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDD 300
           VDGWLVYAPDLPWF +DSGNA+NILDL G+VSELPESM EFE PPSP SSYDNQY NEDD
Sbjct: 241 VDGWLVYAPDLPWFRNDSGNAYNILDLPGYVSELPESMFEFETPPSPPSSYDNQYFNEDD 300

Query: 301 FSRPPPELPPHLQGTILNDPSSSIDGRQLQVTPRSTELNHLYLQSNVQDQFLALGSTHRI 360
           F RPPPELPP LQGTILNDPSSS+DGR L VTPR+TELNHLYLQ NVQDQFLALGS+ RI
Sbjct: 301 FCRPPPELPPQLQGTILNDPSSSVDGRPLPVTPRNTELNHLYLQRNVQDQFLALGSSQRI 347

Query: 361 REKHVTMFVFKPLSRT 376
            EK+ TMF+FKPLSRT
Sbjct: 361 HEKYYTMFLFKPLSRT 347

BLAST of Spg006156 vs. ExPASy TrEMBL
Match: A0A6J1ERH6 (SNF1-related protein kinase regulatory subunit beta-2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435212 PE=3 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.9e-156
Identity = 285/374 (76.20%), Postives = 309/374 (82.62%), Query Frame = 0

Query: 4   LEMSLLKIYIEHIAVRMGQRKKKQTHVDAPPSGGVSISNLVMSTFNHQTLNSIRKRRAVE 63
           LEMSLLK+YIEHIAVRMGQ KKKQTHVDAPP GGVS SNLV+STFNH TLN     R++ 
Sbjct: 11  LEMSLLKVYIEHIAVRMGQGKKKQTHVDAPPGGGVSFSNLVISTFNHLTLNFKEGSRSLR 70

Query: 64  VYDNSDLDCRLLFKSWFSFCFSVMDNAEDRKNGEGPSGAHKFQENHDQRMDCADDAAHNS 123
                                 +MDNAE  KN +  SG HKF+ENHDQRMDC +DA  NS
Sbjct: 71  ----------------------LMDNAEGSKNRDDLSGVHKFEENHDQRMDCTEDAIQNS 130

Query: 124 YHTYTFSESRIQISPQSLGSPQALISNPQYATVSLSSPGLMLQPQPQSLPQS-AAFSENV 183
           +HT +F++SRIQISPQSLGS       PQYATVSLSSPGLMLQPQPQS+PQS AAF ENV
Sbjct: 131 HHTRSFTDSRIQISPQSLGS-------PQYATVSLSSPGLMLQPQPQSVPQSAAAFIENV 190

Query: 184 FHETRTRVRISWNHGGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFIVDG 243
           FHETR+RVRISWNHGGNQVAIVGSWDNWQ+RELLQNTGEKFVV+KTLPVGIYHYHFIVDG
Sbjct: 191 FHETRSRVRISWNHGGNQVAIVGSWDNWQSRELLQNTGEKFVVVKTLPVGIYHYHFIVDG 250

Query: 244 WLVYAPDLPWFCDDSGNAFNILDLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDDFSR 303
           WLVYAPDLPWF +DSGNA+NILDL G+VSELPESM EFE PPSP SSYDNQY NEDDF R
Sbjct: 251 WLVYAPDLPWFRNDSGNAYNILDLPGYVSELPESMFEFETPPSPPSSYDNQYFNEDDFCR 310

Query: 304 PPPELPPHLQGTILNDPSSSIDGRQLQVTPRSTELNHLYLQSNVQDQFLALGSTHRIREK 363
           PPPELPP LQGTILNDPSSS+DGR L VTPR+TELNHLYLQ NVQDQFLALGS+ RI EK
Sbjct: 311 PPPELPPQLQGTILNDPSSSVDGRPLPVTPRNTELNHLYLQRNVQDQFLALGSSQRIHEK 355

Query: 364 HVTMFVFKPLSRTR 377
           + TMF+FKPLSRTR
Sbjct: 371 YYTMFLFKPLSRTR 355

BLAST of Spg006156 vs. TAIR 10
Match: AT4G16360.1 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 196.8 bits (499), Expect = 3.1e-50
Identity = 104/239 (43.51%), Postives = 145/239 (60.67%), Query Frame = 0

Query: 137 SPQSLGSPQALISNPQYATVSLSSPGLMLQPQPQSLPQSAAFSENVFHETRTRVRISWNH 196
           SP++  SP  L+  PQ   + L  P  +  P P  +   ++  E   +E      I+W H
Sbjct: 23  SPRATQSP--LMFAPQVPVLPLQRPDEIHIPNPSWMQSPSSLYEEASNEQGIPTMITWCH 82

Query: 197 GGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFIVDGWLVYAPDLPWFCDD 256
           GG ++A+ GSWDNW+TR  LQ +G+ F ++K LP G+Y Y FIVDG   +AP+LP   DD
Sbjct: 83  GGKEIAVEGSWDNWKTRSRLQRSGKDFTIMKVLPSGVYEYRFIVDGQWRHAPELPLARDD 142

Query: 257 SGNAFNILDLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDDFSRPPPELPPHLQGTIL 316
           +GN FNILDLQ +V E  +S+S FE P SP +SY N  L  +D+S+ PP +PPHLQ T+L
Sbjct: 143 AGNTFNILDLQDYVPEDIQSISGFEPPQSPENSYSNLLLGAEDYSKEPPVVPPHLQMTLL 202

Query: 317 NDPSSSIDGRQLQVTPRSTELNHLYLQSNVQ-DQFLALGSTHRIREKHVTMFVFKPLSR 375
           N P+++ D       P+   LNHLY+Q        +ALGSTHR   K+VT+ ++K L R
Sbjct: 203 NLPAANPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALGSTHRFLAKYVTVVLYKSLQR 259

BLAST of Spg006156 vs. TAIR 10
Match: AT4G16360.3 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 196.8 bits (499), Expect = 3.1e-50
Identity = 104/239 (43.51%), Postives = 145/239 (60.67%), Query Frame = 0

Query: 137 SPQSLGSPQALISNPQYATVSLSSPGLMLQPQPQSLPQSAAFSENVFHETRTRVRISWNH 196
           SP++  SP  L+  PQ   + L  P  +  P P  +   ++  E   +E      I+W H
Sbjct: 25  SPRATQSP--LMFAPQVPVLPLQRPDEIHIPNPSWMQSPSSLYEEASNEQGIPTMITWCH 84

Query: 197 GGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFIVDGWLVYAPDLPWFCDD 256
           GG ++A+ GSWDNW+TR  LQ +G+ F ++K LP G+Y Y FIVDG   +AP+LP   DD
Sbjct: 85  GGKEIAVEGSWDNWKTRSRLQRSGKDFTIMKVLPSGVYEYRFIVDGQWRHAPELPLARDD 144

Query: 257 SGNAFNILDLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDDFSRPPPELPPHLQGTIL 316
           +GN FNILDLQ +V E  +S+S FE P SP +SY N  L  +D+S+ PP +PPHLQ T+L
Sbjct: 145 AGNTFNILDLQDYVPEDIQSISGFEPPQSPENSYSNLLLGAEDYSKEPPVVPPHLQMTLL 204

Query: 317 NDPSSSIDGRQLQVTPRSTELNHLYLQSNVQ-DQFLALGSTHRIREKHVTMFVFKPLSR 375
           N P+++ D       P+   LNHLY+Q        +ALGSTHR   K+VT+ ++K L R
Sbjct: 205 NLPAANPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALGSTHRFLAKYVTVVLYKSLQR 261

BLAST of Spg006156 vs. TAIR 10
Match: AT4G16360.2 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 190.7 bits (483), Expect = 2.2e-48
Identity = 103/239 (43.10%), Postives = 144/239 (60.25%), Query Frame = 0

Query: 137 SPQSLGSPQALISNPQYATVSLSSPGLMLQPQPQSLPQSAAFSENVFHETRTRVRISWNH 196
           SP++  SP  L+  PQ   + L  P  +  P P  +   ++  E   +E      I+W H
Sbjct: 23  SPRATQSP--LMFAPQVPVLPLQRPDEIHIPNPSWMQSPSSLYEEASNEQGIPTMITWCH 82

Query: 197 GGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFIVDGWLVYAPDLPWFCDD 256
           GG ++A+ GSWDNW+T   LQ +G+ F ++K LP G+Y Y FIVDG   +AP+LP   DD
Sbjct: 83  GGKEIAVEGSWDNWKTSR-LQRSGKDFTIMKVLPSGVYEYRFIVDGQWRHAPELPLARDD 142

Query: 257 SGNAFNILDLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDDFSRPPPELPPHLQGTIL 316
           +GN FNILDLQ +V E  +S+S FE P SP +SY N  L  +D+S+ PP +PPHLQ T+L
Sbjct: 143 AGNTFNILDLQDYVPEDIQSISGFEPPQSPENSYSNLLLGAEDYSKEPPVVPPHLQMTLL 202

Query: 317 NDPSSSIDGRQLQVTPRSTELNHLYLQSNVQ-DQFLALGSTHRIREKHVTMFVFKPLSR 375
           N P+++ D       P+   LNHLY+Q        +ALGSTHR   K+VT+ ++K L R
Sbjct: 203 NLPAANPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALGSTHRFLAKYVTVVLYKSLQR 258

BLAST of Spg006156 vs. TAIR 10
Match: AT5G21170.2 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 163.7 bits (413), Expect = 2.9e-40
Identity = 80/184 (43.48%), Postives = 114/184 (61.96%), Query Frame = 0

Query: 192 ISWNHGGNQVAIVGSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFIVDGWLVYAPDLP 251
           I+WN GGN VA+ GSWDNW++R+ LQ +G+   ++  LP GIYHY  IVDG   Y PDLP
Sbjct: 141 ITWNQGGNDVAVEGSWDNWRSRKKLQKSGKDHSILFVLPSGIYHYKVIVDGESKYIPDLP 200

Query: 252 WFCDDSGNAFNILDLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDDFSRPPPELPPHL 311
           +  D+ GN  NILD+   V E PES+ EFEAPPSP  SY       +D+++ P  +PP L
Sbjct: 201 FVADEVGNVCNILDVHNFVPENPESIVEFEAPPSPDHSYGQTLPAAEDYAKEPLAVPPQL 260

Query: 312 QGTILNDPSSSIDGRQLQVTPRSTELNHLYLQSNVQDQ-FLALGSTHRIREKHVTMFVFK 371
             T+L     + +   +   P+   LNH++++     Q  +ALG THR   K++T+ ++K
Sbjct: 261 HLTLL----GTTEETAIATKPQHVVLNHVFIEQGWTPQSIVALGLTHRFESKYITVVLYK 320

Query: 372 PLSR 375
           PL+R
Sbjct: 321 PLTR 320

BLAST of Spg006156 vs. TAIR 10
Match: AT5G21170.1 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 161.4 bits (407), Expect = 1.4e-39
Identity = 105/291 (36.08%), Postives = 149/291 (51.20%), Query Frame = 0

Query: 89  NAEDRKNGEGPSGAHKFQENHDQRMDCADDAAHNSYHTYTFSESRIQISPQS-LGSPQAL 148
           N +D     G  GA     +   R +  D +A  S H    S+S     P S   SP   
Sbjct: 5   NGKDEDAAAGSGGAD--VTSSSARSNGGDPSA-RSRHRRPSSDSMSSSPPGSPARSPSPF 64

Query: 149 ISNPQYATVSLSSPGLMLQPQPQSLPQSAAFSENVFH---ETRTRVRISWNHGGNQVAIV 208
           +  PQ     L        P P ++  +   S+ VF    E      I+WN GGN VA+ 
Sbjct: 65  LFAPQVPVAPLQRAN---APPPNNIQWNQ--SQRVFDNPPEQGIPTIITWNQGGNDVAVE 124

Query: 209 GSWDNWQTRELLQNTGEKFVVIKTLPVGIYHYHFIVDGWLVYAPDLPWFCDDSGNAFNIL 268
           GSWDNW++R+ LQ +G+   ++  LP GIYHY  IVDG   Y PDLP+  D+ GN  NIL
Sbjct: 125 GSWDNWRSRKKLQKSGKDHSILFVLPSGIYHYKVIVDGESKYIPDLPFVADEVGNVCNIL 184

Query: 269 DLQGHVSELPESMSEFEAPPSPRSSYDNQYLNEDDFSRPPPELPPHLQGTILNDPSSSID 328
           D+   V E PES+ EFEAPPSP  SY       +D+++ P  +PP L  T+L     + +
Sbjct: 185 DVHNFVPENPESIVEFEAPPSPDHSYGQTLPAAEDYAKEPLAVPPQLHLTLL----GTTE 244

Query: 329 GRQLQVTPRSTELNHLYLQSNVQDQ-FLALGSTHRIREKHVTMFVFKPLSR 375
              +   P+   LNH++++     Q  +ALG THR   K++T+ ++KPL+R
Sbjct: 245 ETAIATKPQHVVLNHVFIEQGWTPQSIVALGLTHRFESKYITVVLYKPLTR 283

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022928365.11.6e-15776.39SNF1-related protein kinase regulatory subunit beta-2-like isoform X7 [Cucurbita... [more]
XP_022928361.11.6e-15776.39SNF1-related protein kinase regulatory subunit beta-2-like isoform X3 [Cucurbita... [more]
XP_022928364.14.6e-15776.33SNF1-related protein kinase regulatory subunit beta-2-like isoform X6 [Cucurbita... [more]
XP_022928358.14.6e-15776.33SNF1-related protein kinase regulatory subunit beta-2-like isoform X1 [Cucurbita... [more]
XP_022928360.13.9e-15676.20SNF1-related protein kinase regulatory subunit beta-2-like isoform X2 [Cucurbita... [more]
Match NameE-valueIdentityDescription
Q9SCY54.3e-4943.51SNF1-related protein kinase regulatory subunit beta-2 OS=Arabidopsis thaliana OX... [more]
Q84VQ12.0e-3836.08SNF1-related protein kinase regulatory subunit beta-1 OS=Arabidopsis thaliana OX... [more]
O437413.2e-2033.515'-AMP-activated protein kinase subunit beta-2 OS=Homo sapiens OX=9606 GN=PRKAB2... [more]
Q6PAM04.2e-2033.515'-AMP-activated protein kinase subunit beta-2 OS=Mus musculus OX=10090 GN=Prkab... [more]
Q9QZH49.4e-2033.515'-AMP-activated protein kinase subunit beta-2 OS=Rattus norvegicus OX=10116 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1ERI17.7e-15876.39SNF1-related protein kinase regulatory subunit beta-2-like isoform X7 OS=Cucurbi... [more]
A0A6J1EK377.7e-15876.39SNF1-related protein kinase regulatory subunit beta-2-like isoform X3 OS=Cucurbi... [more]
A0A6J1ENS92.2e-15776.33SNF1-related protein kinase regulatory subunit beta-2-like isoform X1 OS=Cucurbi... [more]
A0A6J1EKM32.2e-15776.33SNF1-related protein kinase regulatory subunit beta-2-like isoform X6 OS=Cucurbi... [more]
A0A6J1ERH61.9e-15676.20SNF1-related protein kinase regulatory subunit beta-2-like isoform X2 OS=Cucurbi... [more]
Match NameE-valueIdentityDescription
AT4G16360.13.1e-5043.515'-AMP-activated protein kinase beta-2 subunit protein [more]
AT4G16360.33.1e-5043.515'-AMP-activated protein kinase beta-2 subunit protein [more]
AT4G16360.22.2e-4843.105'-AMP-activated protein kinase beta-2 subunit protein [more]
AT5G21170.22.9e-4043.485'-AMP-activated protein kinase beta-2 subunit protein [more]
AT5G21170.11.4e-3936.085'-AMP-activated protein kinase beta-2 subunit protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006828Association with the SNF1 complex (ASC) domainSMARTSM01010AMPKBI_2coord: 283..372
e-value: 3.3E-12
score: 56.5
IPR006828Association with the SNF1 complex (ASC) domainPFAMPF04739AMPKBIcoord: 302..371
e-value: 2.9E-15
score: 56.3
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 182..270
e-value: 2.6E-19
score: 70.8
NoneNo IPR availableGENE3D6.20.250.60coord: 276..372
e-value: 2.9E-21
score: 77.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 282..296
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 275..311
NoneNo IPR availablePANTHERPTHR46316:SF6SNF1-RELATED KINASE REGULATORY SUBUNIT BETA-2coord: 87..375
NoneNo IPR availableCDDcd02859E_set_AMPKbeta_like_Ncoord: 190..265
e-value: 9.13977E-19
score: 78.0244
IPR032640AMP-activated protein kinase, glycogen-binding domainPFAMPF16561AMPK1_CBMcoord: 190..267
e-value: 1.7E-23
score: 82.6
IPR043554SNF1-related protein kinase regulatory subunit betaPANTHERPTHR46316SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-1coord: 87..375
IPR014756Immunoglobulin E-setSUPERFAMILY81296E set domainscoord: 188..266
IPR037256ASC domain superfamilySUPERFAMILY160219AMPKBI-likecoord: 270..372

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg006156.1Spg006156.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005515 protein binding