Spg006103 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg006103
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionExopolygalacturonase-like
Locationscaffold4: 5589622 .. 5591332 (-)
RNA-Seq ExpressionSpg006103
SyntenySpg006103
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTAGCAATCTGAACAAGGAGAAAATAAGCAAAGTAGAAGGTACATACATAAAGGATGGGATTCAAAACAAACTTTGTAGCATTACTACCTTTGCTATTATTGTTGGTGTCTACTGCTAATGGTCAATATGTTTTTGATGTCACTAAGTATGGTGCCAAACCTAATGCTGATATTACTCAAGTAAGTATATATTAGTTTGACAAACCATTTTAATTTATCAAAAAAATATTTTAATTTAATGAAACAATTCAATTTACAGGCTTTAGCAAATGCATGGAAGGAAGCATGTGCGTCGACAACTCCAAGTAAAGTGTTGGTTCCAAAAGGAGTTTACCAATTAAGTCAATCCAACCTCAAAGGCCCTTGCAAGAGTCCTATTGAGTTTCAAGTCGAAGGGACGTTACAAGCTCCTCCCCACCCAACAGGAGATGGGTTGGTTCTTTTGGAATATATTGATCAATTGACATTGTCAGGCACTGGAGTGTTTGACGGTCAAGGAAAAGCAGGTTGGGAAAAGAATGATTGCCACCAGAAGAAAATATGTACCAAGCTTCCCATGGTAAACGCTCTAATATAGTGGTCTTTTGTTTTTGGCCTAATATCATACGTTATTTAATCACAAATAATCTCAATTAACATAAATTTTTTAACTCCGAAAGCTTTTTTTTTTTTTTTAAATGAGCGCAGACCTTGAAGTTCAATTTCGTCACCAATTCGTTAGTGAAGGACATAACTTCATTGGATAGTAAGAATTTTCATATCAATCTTTTGGGCTGCAAGAATCTTACTTTTCAACACGTGACAATTACTGCACCAGAGGATAGCCCCAACACGGACGGAATTCATCTGAGTAGTTCATAACAGATTAACATTCTCGATACTAAAATCGCTACTGGAGATGATTGTGTATCTGTAGGAGATAGTAACAAACAAATAACTATTACTAACGTAACTTGCGGACCAGGACATGGCATTAGCATAGGAAGTTTGGGCAAGTACACAAAGGAAAAAGACGTGGTTGGAGTTTTCGTGAAAAAATGCAAAATAATGAGTACTACAAATGGCGTCAGGATCAAAACATGGCCAGATTCTGCCGGTGCATTCTCAGCCACTGACATGCATTTTGAAGATATTGAAATGGTTAACGTCAGCAATCCGGTCATTATAGACCAAGAATATTGTCCATGGAATCAATGCAACCGAAAGGTATATATATTTTGGAATTATTATGTGTCATTCATTTTCAACTTATTATTAACTTTAAGAAGTACTATAAAAACTAATGCAAAGTTGAGTGATTATTATGTTAATAAATATATATATTTTTGGTACCTTTAGGTTGCATCAAAAATTAAGATCAGCAAAGTTAGCTTCAAGAATATCAGAGGCACTTCTGCTACTCCAGTCGCGGTCAAATTTGTTTGCAGCAAAAGTCTACCATGTGAAGGTGTAGAAGTTGCTGATATTGACCTCACTTACAATGGAACCCAAGGACCGATTAAGTCTCAGTGTTCAAATGTGAAACCCACCATTACTGGAAAGCAAAACCCTCCCATATGTGCTGAACCTGCCCCTATTGATTCTCCATCAACTGACTAATCTTGTTCACAGTGACAAAAATTACCAAACCATGAGAAATTCTTTTTTTTTTTCGCGACATTTTTGTTTTACATTCAAAATTCTTTAGGTCTACTCCATTAAATAACGC

mRNA sequence

ATGGGATTCAAAACAAACTTTGTAGCATTACTACCTTTGCTATTATTGTTGGTGTCTACTGCTAATGGTCAATATGTTTTTGATGTCACTAAGTATGGTGCCAAACCTAATGCTGATATTACTCAAGCTTTAGCAAATGCATGGAAGGAAGCATGTGCGTCGACAACTCCAAGTAAAGTGTTGGTTCCAAAAGGAGTTTACCAATTAAGTCAATCCAACCTCAAAGGCCCTTGCAAGAGTCCTATTGAGTTTCAAGTCGAAGGGACGTTACAAGCTCCTCCCCACCCAACAGGAGATGGGTTGGTTCTTTTGGAATATATTGATCAATTGACATTGTCAGGCACTGGAGTGTTTGACGGTCAAGGAAAAGCAGGTTGGGAAAAGAATGATTGCCACCAGAAGAAAATATGTACCAAGCTTCCCATGATTAACATTCTCGATACTAAAATCGCTACTGGAGATGATTGTGTATCTGTAGGAGATAGTAACAAACAAATAACTATTACTAACGTAACTTGCGGACCAGGACATGGCATTAGCATAGGAAGTTTGGGCAAGTACACAAAGGAAAAAGACGTGGTTGGAGTTTTCGTGAAAAAATGCAAAATAATGAGTACTACAAATGGCGTCAGGATCAAAACATGGCCAGATTCTGCCGGTGCATTCTCAGCCACTGACATGCATTTTGAAGATATTGAAATGGTTAACGTCAGCAATCCGGTCATTATAGACCAAGAATATTGTCCATGGAATCAATGCAACCGAAAGGTTGCATCAAAAATTAAGATCAGCAAAGTTAGCTTCAAGAATATCAGAGGCACTTCTGCTACTCCAGTCGCGGTCAAATTTGTTTGCAGCAAAAGTCTACCATGTGAAGGTGTAGAAGTTGCTGATATTGACCTCACTTACAATGGAACCCAAGGACCGATTAAGTCTCAGTGTTCAAATGTGAAACCCACCATTACTGGAAAGCAAAACCCTCCCATATGTGCTGAACCTGCCCCTATTGATTCTCCATCAACTGACTAA

Coding sequence (CDS)

ATGGGATTCAAAACAAACTTTGTAGCATTACTACCTTTGCTATTATTGTTGGTGTCTACTGCTAATGGTCAATATGTTTTTGATGTCACTAAGTATGGTGCCAAACCTAATGCTGATATTACTCAAGCTTTAGCAAATGCATGGAAGGAAGCATGTGCGTCGACAACTCCAAGTAAAGTGTTGGTTCCAAAAGGAGTTTACCAATTAAGTCAATCCAACCTCAAAGGCCCTTGCAAGAGTCCTATTGAGTTTCAAGTCGAAGGGACGTTACAAGCTCCTCCCCACCCAACAGGAGATGGGTTGGTTCTTTTGGAATATATTGATCAATTGACATTGTCAGGCACTGGAGTGTTTGACGGTCAAGGAAAAGCAGGTTGGGAAAAGAATGATTGCCACCAGAAGAAAATATGTACCAAGCTTCCCATGATTAACATTCTCGATACTAAAATCGCTACTGGAGATGATTGTGTATCTGTAGGAGATAGTAACAAACAAATAACTATTACTAACGTAACTTGCGGACCAGGACATGGCATTAGCATAGGAAGTTTGGGCAAGTACACAAAGGAAAAAGACGTGGTTGGAGTTTTCGTGAAAAAATGCAAAATAATGAGTACTACAAATGGCGTCAGGATCAAAACATGGCCAGATTCTGCCGGTGCATTCTCAGCCACTGACATGCATTTTGAAGATATTGAAATGGTTAACGTCAGCAATCCGGTCATTATAGACCAAGAATATTGTCCATGGAATCAATGCAACCGAAAGGTTGCATCAAAAATTAAGATCAGCAAAGTTAGCTTCAAGAATATCAGAGGCACTTCTGCTACTCCAGTCGCGGTCAAATTTGTTTGCAGCAAAAGTCTACCATGTGAAGGTGTAGAAGTTGCTGATATTGACCTCACTTACAATGGAACCCAAGGACCGATTAAGTCTCAGTGTTCAAATGTGAAACCCACCATTACTGGAAAGCAAAACCCTCCCATATGTGCTGAACCTGCCCCTATTGATTCTCCATCAACTGACTAA

Protein sequence

MGFKTNFVALLPLLLLLVSTANGQYVFDVTKYGAKPNADITQALANAWKEACASTTPSKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPPHPTGDGLVLLEYIDQLTLSGTGVFDGQGKAGWEKNDCHQKKICTKLPMINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVADIDLTYNGTQGPIKSQCSNVKPTITGKQNPPICAEPAPIDSPSTD
Homology
BLAST of Spg006103 vs. NCBI nr
Match: XP_011652893.2 (exopolygalacturonase [Cucumis sativus] >KGN64588.2 hypothetical protein Csa_014082 [Cucumis sativus])

HSP 1 Score: 561.6 bits (1446), Expect = 4.6e-156
Identity = 285/406 (70.20%), Postives = 307/406 (75.62%), Query Frame = 0

Query: 1   MGFKTNFVALLPLLLLLVSTANGQY-----VFDVTKYGAKPNADITQALANAWKEACAST 60
           MG ++NFV LLPL LLLVS A GQ      VFDVT +GAKPN DIT  L  AWK+ACAST
Sbjct: 1   MGLRSNFVTLLPLFLLLVSNAKGQSAAGGGVFDVTSFGAKPNGDITGPLLVAWKDACAST 60

Query: 61  TPSKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPPHPTGDGLVLLEYIDQLTLSGT 120
           TPSKVL+PKG Y LSQSNLKGPCKSPIE Q+EGTLQA P P GDGL+LLEYIDQLT+SGT
Sbjct: 61  TPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQASPDPKGDGLLLLEYIDQLTVSGT 120

Query: 121 GVFDGQGKAGWEKNDCHQKKICTKLPM--------------------------------- 180
           GV DGQGKAGWEKNDCH KKICTKLPM                                 
Sbjct: 121 GVLDGQGKAGWEKNDCHLKKICTKLPMTLKLNFITNSIVKDITSLDSKNYHINLLGCKNL 180

Query: 181 --------------------------INILDTKIATGDDCVSVGDSNKQITITNVTCGPG 240
                                     INIL+T IATGDDC+SVGD+NKQI I++VTCGPG
Sbjct: 181 TIDHVTITAPENSPNTDGIHVSSSEQINILNTNIATGDDCISVGDTNKQIVISDVTCGPG 240

Query: 241 HGISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVN 300
           HGISIGSLGKY+KEK+VVGV VKKCK+ STTNGVRIKTWPDS G F ATDMHFEDIEMVN
Sbjct: 241 HGISIGSLGKYSKEKEVVGVTVKKCKLTSTTNGVRIKTWPDSVGTFPATDMHFEDIEMVN 300

Query: 301 VSNPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEV 343
           VSNP+IIDQEYCPWNQCNRKV SKIKISKVSFKNIRGTSAT VAVK VCS+S PCE VEV
Sbjct: 301 VSNPIIIDQEYCPWNQCNRKVPSKIKISKVSFKNIRGTSATAVAVKIVCSQSNPCEEVEV 360

BLAST of Spg006103 vs. NCBI nr
Match: KAA0045593.1 (exopolygalacturonase-like [Cucumis melo var. makuwa] >TYK28803.1 exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 555.4 bits (1430), Expect = 3.3e-154
Identity = 280/404 (69.31%), Postives = 308/404 (76.24%), Query Frame = 0

Query: 1   MGFKTNFVALLPLLLLLVSTANGQ---YVFDVTKYGAKPNADITQALANAWKEACASTTP 60
           MG  +NFVALLP+LLLLVS +  Q    VFD+TKYGAKPNAD+T AL +AWKEACASTTP
Sbjct: 1   MGLISNFVALLPVLLLLVSNSKAQSSGSVFDITKYGAKPNADVTAALVSAWKEACASTTP 60

Query: 61  SKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPPHPTGDGLVLLEYIDQLTLSGTGV 120
           SKVL+PKG Y LSQSNLKGPCKS IE Q+EGTLQAP  P GDGL+LLEY+DQLT+SGTGV
Sbjct: 61  SKVLIPKGSYGLSQSNLKGPCKSAIELQIEGTLQAPADPKGDGLLLLEYVDQLTVSGTGV 120

Query: 121 FDGQGKAGWEKNDCHQKKICTKLPM----------------------------------- 180
            DGQGKAGWEKNDCH KK CTKLPM                                   
Sbjct: 121 LDGQGKAGWEKNDCHLKKECTKLPMNLKLNFVTNSIVKDITSLDSKNYHINLLGCKNLTF 180

Query: 181 ------------------------INILDTKIATGDDCVSVGDSNKQITITNVTCGPGHG 240
                                   INIL+T IATGDDC+SVGD+NKQ+ I++VTCGPGHG
Sbjct: 181 DHVTITAPGNSPNTDGIHVSSSEQINILNTNIATGDDCISVGDTNKQVVISDVTCGPGHG 240

Query: 241 ISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVS 300
           ISIGSLGKY+KEK+VVGV VKKCK+  TTNGVRIKTWPDSA AF A+DMHFEDIEMVNVS
Sbjct: 241 ISIGSLGKYSKEKEVVGVTVKKCKLTDTTNGVRIKTWPDSAVAFPASDMHFEDIEMVNVS 300

Query: 301 NPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVAD 343
           NPVIIDQEYCPWNQCNRKV SKIKIS VSFKNIRGTSATPVAVK VCS+S PCEGVEVAD
Sbjct: 301 NPVIIDQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKIVCSQSNPCEGVEVAD 360

BLAST of Spg006103 vs. NCBI nr
Match: KAA0045608.1 (exopolygalacturonase-like [Cucumis melo var. makuwa] >TYK02648.1 exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 555.4 bits (1430), Expect = 3.3e-154
Identity = 280/404 (69.31%), Postives = 308/404 (76.24%), Query Frame = 0

Query: 1   MGFKTNFVALLPLLLLLVSTANGQY---VFDVTKYGAKPNADITQALANAWKEACASTTP 60
           MG  +NFV LLP+LLLLVS +  Q    VFD+TKYGAKPNAD+T ALA+AWKEACASTTP
Sbjct: 1   MGLISNFVTLLPVLLLLVSNSKAQSSGGVFDITKYGAKPNADVTAALASAWKEACASTTP 60

Query: 61  SKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPPHPTGDGLVLLEYIDQLTLSGTGV 120
           SKVL+PKG Y LSQSNLKGPCKS IE Q+EGTLQAP  P GDGL+LLEY+DQLT+SGTGV
Sbjct: 61  SKVLIPKGSYGLSQSNLKGPCKSAIELQIEGTLQAPADPKGDGLLLLEYVDQLTVSGTGV 120

Query: 121 FDGQGKAGWEKNDCHQKKICTKLPM----------------------------------- 180
            DGQGKAGWEKNDCH KK CTKLPM                                   
Sbjct: 121 LDGQGKAGWEKNDCHLKKECTKLPMNLKLNFVTNSIVKDITSLDSKNYHINLLGCKNLTF 180

Query: 181 ------------------------INILDTKIATGDDCVSVGDSNKQITITNVTCGPGHG 240
                                   INIL+T IATGDDC+SVGD+NKQ+ I++VTCGPGHG
Sbjct: 181 DHVTITAPGNSPNTDGIHVSSSEQINILNTNIATGDDCISVGDTNKQVVISDVTCGPGHG 240

Query: 241 ISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVS 300
           ISIGSLGKY+KEK+VVGV VKKCK+  TTNGVRIKTWPDSA AF A+DMHFEDIEMVNVS
Sbjct: 241 ISIGSLGKYSKEKEVVGVTVKKCKLTDTTNGVRIKTWPDSAVAFPASDMHFEDIEMVNVS 300

Query: 301 NPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVAD 343
           NPVIIDQEYCPWNQCNRKV SKIKIS VSFKNIRGTSATPVAVK VCS+S PCEGVEVAD
Sbjct: 301 NPVIIDQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKIVCSQSNPCEGVEVAD 360

BLAST of Spg006103 vs. NCBI nr
Match: XP_031744817.1 (exopolygalacturonase-like [Cucumis sativus] >KAE8645715.1 hypothetical protein Csa_020330 [Cucumis sativus])

HSP 1 Score: 554.7 bits (1428), Expect = 5.7e-154
Identity = 278/395 (70.38%), Postives = 307/395 (77.72%), Query Frame = 0

Query: 8   VALLPLLLLLVSTANGQYVFDVTKYGAKPNADITQALANAWKEACASTTPSKVLVPKGVY 67
           +ALLP  LLL ST   Q +FDVT+YGAKPN DITQALANAWKEAC STTPSK+L+PKGVY
Sbjct: 3   LALLPFFLLLASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIPKGVY 62

Query: 68  QLSQSNLKGPCKS-PIEFQVEGTLQAPPHPTGDGLVLLEYIDQLTLSGTGVFDGQGKAGW 127
           QLSQS LKGPCKS PI+ QV+GTL+AP HP GDGLVL  YIDQL LSGTGVFDGQGKAGW
Sbjct: 63  QLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQGKAGW 122

Query: 128 EKNDCHQKKICTKLPM-------------------------------------------- 187
           +KNDCH+KKICTKLPM                                            
Sbjct: 123 DKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTISAPE 182

Query: 188 ---------------INILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISIGSLGKY 247
                          INILD+KI+TGDDCVSVGDSNKQ+TITNVTCGPGHGIS+GSLGKY
Sbjct: 183 NSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGSLGKY 242

Query: 248 TKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPVIIDQEY 307
           TKEKDVVGV VK CK+++TTNGVRIKTWPDSAGAF A+DMHFEDIEM NVSNPVIIDQEY
Sbjct: 243 TKEKDVVGVSVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVIIDQEY 302

Query: 308 CPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVADIDLTYNGTQ 343
           CPWNQCNRKV SKIKISKVSFKNIRGTSATP+AVK VCSKS+PCEGVEVADI+LTY+G +
Sbjct: 303 CPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTYSGIR 362

BLAST of Spg006103 vs. NCBI nr
Match: XP_031744814.1 (exopolygalacturonase-like [Cucumis sativus] >XP_031744815.1 LOW QUALITY PROTEIN: exopolygalacturonase [Cucumis sativus] >XP_031744816.1 exopolygalacturonase-like [Cucumis sativus] >XP_031744818.1 exopolygalacturonase-like [Cucumis sativus] >KAE8645716.1 hypothetical protein Csa_020252 [Cucumis sativus])

HSP 1 Score: 554.7 bits (1428), Expect = 5.7e-154
Identity = 278/395 (70.38%), Postives = 307/395 (77.72%), Query Frame = 0

Query: 8   VALLPLLLLLVSTANGQYVFDVTKYGAKPNADITQALANAWKEACASTTPSKVLVPKGVY 67
           +ALLP  LLL ST   Q +FDVT+YGAKPN DITQALANAWKEAC STTPSK+L+PKGVY
Sbjct: 3   LALLPFFLLLASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIPKGVY 62

Query: 68  QLSQSNLKGPCKS-PIEFQVEGTLQAPPHPTGDGLVLLEYIDQLTLSGTGVFDGQGKAGW 127
           QLSQS LKGPCKS PI+ QV+GTL+AP HP GDGLVL  YIDQL LSGTGVFDGQGKAGW
Sbjct: 63  QLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQGKAGW 122

Query: 128 EKNDCHQKKICTKLPM-------------------------------------------- 187
           +KNDCH+KKICTKLPM                                            
Sbjct: 123 DKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTISAPE 182

Query: 188 ---------------INILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISIGSLGKY 247
                          INILD+KI+TGDDCVSVGDSNKQ+TITNVTCGPGHGIS+GSLGKY
Sbjct: 183 NSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGSLGKY 242

Query: 248 TKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPVIIDQEY 307
           TKEKDVVGV VK CK+++TTNGVRIKTWPDSAGAF A+DMHFEDIEM NVSNPVIIDQEY
Sbjct: 243 TKEKDVVGVTVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVIIDQEY 302

Query: 308 CPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVADIDLTYNGTQ 343
           CPWNQCNRKV SKIKISKVSFKNIRGTSATP+AVK VCSKS+PCEGVEVADI+LTY+G +
Sbjct: 303 CPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTYSGIR 362

BLAST of Spg006103 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 3.9e-97
Identity = 186/399 (46.62%), Postives = 241/399 (60.40%), Query Frame = 0

Query: 3   FKTNFVALLPLLLLLVSTANGQYVFDVTKYGAKPNADITQALANAWKEACASTTPSKVLV 62
           FK +F  +L  L     +  G  VFD+TKYGA  NADI++AL NA+KEAC ST+PS +++
Sbjct: 5   FKVHFALVLLFLAHFGESQTG--VFDITKYGANSNADISEALLNAFKEACQSTSPSTIVI 64

Query: 63  PKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPPHPT----GDGLVLLEYIDQLTLSGTGVF 122
           PKG + ++Q  L+GPCKSP+E Q++ TL+AP  P+    G+ L  +  +DQ T+SG G+ 
Sbjct: 65  PKGTFTMNQVKLEGPCKSPLELQIQATLKAPSDPSQLKVGEWLT-VNKLDQFTMSGGGIL 124

Query: 123 DGQGKAGWEKNDCHQKKICTKLP------------------------------------- 182
           DGQ  A WE   C Q K C KLP                                     
Sbjct: 125 DGQAAAAWE---CKQSKKCNKLPNNLSFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTFI 184

Query: 183 ----------------------MINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGI 242
                                  +NI D+  +TGDDC+SVGD  +Q+ IT VTCGPGHGI
Sbjct: 185 RFNVSAPANSPNTDGIHVSRSSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPGHGI 244

Query: 243 SIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSN 302
           S+GSLG    EK VVGVFV+ C   +T NGVRIKTWP S       D+HFEDI + NVSN
Sbjct: 245 SVGSLGGNPDEKPVVGVFVRNCTFTNTDNGVRIKTWPASHPGV-VNDVHFEDIIVQNVSN 304

Query: 303 PVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVADI 339
           PV+IDQ YCP+N+CN+ + S++KISKVSF+NI+GTS T  AV  + SK +PCEG+EV DI
Sbjct: 305 PVVIDQVYCPFNKCNKDLPSQVKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIEVGDI 364

BLAST of Spg006103 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.5e-93
Identity = 177/359 (49.30%), Postives = 221/359 (61.56%), Query Frame = 0

Query: 39  DITQALANAWKEACASTTPSKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPPHPT- 98
           D TQAL  AWKEACAS +PS +LVPKG + +    L+GPCKS I  Q++GTL+AP  P+ 
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSK 60

Query: 99  --GDGLVLLEYIDQLTLSGTGVFDGQGKAGWEKNDCHQK-KICTKLPM------------ 158
             G G + L  ID LT+ G GVFDGQGK+ W +NDCH+   IC  L M            
Sbjct: 61  IKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSIL 120

Query: 159 -----------------------------------------------INILDTKIATGDD 218
                                                          +NI++T+I TGDD
Sbjct: 121 RDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDD 180

Query: 219 CVSVGDSNKQITITNVTCGPGHGISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTW 278
           C+S+GD +K I ITN+TCGPGHGIS+GSLG+Y  E+ VVG++VK C I  + NGVRIKTW
Sbjct: 181 CISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTW 240

Query: 279 PDSAGAFSATDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTS 335
           P S     A++MHF+DI M +V  P++IDQ YCP+NQC  +V S +K+SK+SFKNI+GTS
Sbjct: 241 PKSEPG-EASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTS 300

BLAST of Spg006103 vs. ExPASy Swiss-Prot
Match: Q40312 (Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 6.4e-92
Identity = 171/375 (45.60%), Postives = 226/375 (60.27%), Query Frame = 0

Query: 26  VFDVTKYGAKPNADITQALANAWKEACASTTPSKVLVPKGVYQLSQSNLKGPCKSPIEFQ 85
           V D++K+G KPN+DI QAL +AW EACASTT +K+++P G YQL+   LKGPCK+PIE Q
Sbjct: 24  VLDISKFGGKPNSDIGQALTSAWNEACASTTAAKIVIPAGTYQLNGIELKGPCKAPIELQ 83

Query: 86  VEGTLQAPPHPT----GDGLVLLEYIDQLTLSGTGVFDGQG---------KAGWEKNDCH 145
           V+GT+QAP  P+     +      Y+D LTLSG GVFDGQG          + W   + +
Sbjct: 84  VDGTIQAPADPSVIKGTEQWFKFLYMDHLTLSGKGVFDGQGATVYKKAAPASAWSGKNSN 143

Query: 146 QKKICT------------------------------------------------------ 205
            K                                                          
Sbjct: 144 SKVFMNFGFNFVNNSIVRGVTSKDSKNFHVMVFGCKNITFDGFTITAPGDSPNTDGIHMG 203

Query: 206 KLPMINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISIGSLGKYTKEKDVVGVFV 265
           K   + IL+T I TGDDCVS+GD +KQIT+  V CGPGHG+S+GSLGK+T E++V G+ V
Sbjct: 204 KSTDVKILNTNIGTGDDCVSIGDGSKQITVQGVNCGPGHGLSVGSLGKFTTEENVEGITV 263

Query: 266 KKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKVA 325
           K C + +T NGVRIKTWPD+ G  + +D+HFEDI M NV NPVIIDQEY PWNQC++K  
Sbjct: 264 KNCTLTATDNGVRIKTWPDAPGTITVSDIHFEDITMTNVKNPVIIDQEYYPWNQCSKKNP 323

Query: 326 SKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVADIDLTYNGTQGPIKSQCSNVK 334
           SKIK+SK+SFKN++GTS T   V  +CS ++PC+GVE+ ++DL +NG   P  ++C+NVK
Sbjct: 324 SKIKLSKISFKNVKGTSGTAEGVVLICSSAVPCDGVELNNVDLKFNG--APTTAKCTNVK 383

BLAST of Spg006103 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 1.7e-89
Identity = 171/393 (43.51%), Postives = 233/393 (59.29%), Query Frame = 0

Query: 9   ALLPLLLLLVSTANGQY-VFD-VTKYGAKPN--ADITQALANAWKEACASTTPSKVLVPK 68
           ++  LLLL +S +  Q   FD V K+GAK +   D+++   +AWKEACAS TPS V++PK
Sbjct: 10  SMFVLLLLFISASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPK 69

Query: 69  GVYQLSQSNLKGPCKSPIEFQVEGTLQAPPHPTG---DGLVLLEYIDQLTLSGTGVFDGQ 128
           G Y LS+ NL+GPCK+PIE  V+GT+QAP  P+       V    ++   + G G+FDGQ
Sbjct: 70  GTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQ 129

Query: 129 GKAGWEKNDCHQKKICTKLPM--------------------------------------- 188
           G   +EKN C  ++  +KLP+                                       
Sbjct: 130 GSIAYEKNTCENREFRSKLPVNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLERLK 189

Query: 189 --------------------INILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISIG 248
                               +NI+ + I TGDDC+S+GD  K + I  +TCGPGHGISIG
Sbjct: 190 IEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIG 249

Query: 249 SLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPVI 308
           SLGK+  E+ V G+ +  C I +T+NG RIKTWP   G  + +++HFEDI M NVS+P++
Sbjct: 250 SLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGG-AVSEIHFEDITMNNVSSPIL 309

Query: 309 IDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVADIDLT 336
           IDQ+YCPWN+C +   SK+K+S +SFKNIRGTSA P A+KF+CS S PC+ VE+ADID+ 
Sbjct: 310 IDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIK 369

BLAST of Spg006103 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 3.9e-89
Identity = 171/393 (43.51%), Postives = 232/393 (59.03%), Query Frame = 0

Query: 9   ALLPLLLLLVSTANGQ-YVFD-VTKYGAKPN--ADITQALANAWKEACASTTPSKVLVPK 68
           ++  LLLL +S +  Q   FD V K+GAK +   D+++   +AWKEACAS TPS V++PK
Sbjct: 10  SMFVLLLLFISASKVQPDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPK 69

Query: 69  GVYQLSQSNLKGPCKSPIEFQVEGTLQAPPHPTG---DGLVLLEYIDQLTLSGTGVFDGQ 128
           G Y LS+ NL+GPCK+PIE  V+GT+QAP  P+       V    ++   + G G+FDGQ
Sbjct: 70  GTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQ 129

Query: 129 GKAGWEKNDCHQKKICTKLPM--------------------------------------- 188
           G   +EKN C  ++  +KLP+                                       
Sbjct: 130 GSIAYEKNTCENREFRSKLPVNIRFDFVTNALIQDITSKDSKLFHINVFACKNITLERLK 189

Query: 189 --------------------INILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISIG 248
                               +NI+ + I TGDDC+S+GD  K + I  +TCGPGHGISIG
Sbjct: 190 IEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIG 249

Query: 249 SLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPVI 308
           SLGK+  E+ V G+ +  C I +T+NG RIKTWP   G  + +++HFEDI M NVS+P++
Sbjct: 250 SLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGG-AVSEIHFEDITMNNVSSPIL 309

Query: 309 IDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVADIDLT 336
           IDQ+YCPWN+C +   SK+K+S +SFKNIRGTSA P A+KF+CS S PC+ VE+ADID+ 
Sbjct: 310 IDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIQ 369

BLAST of Spg006103 vs. ExPASy TrEMBL
Match: A0A5A7TRZ0 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold280G00600 PE=3 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.6e-154
Identity = 280/404 (69.31%), Postives = 308/404 (76.24%), Query Frame = 0

Query: 1   MGFKTNFVALLPLLLLLVSTANGQY---VFDVTKYGAKPNADITQALANAWKEACASTTP 60
           MG  +NFV LLP+LLLLVS +  Q    VFD+TKYGAKPNAD+T ALA+AWKEACASTTP
Sbjct: 1   MGLISNFVTLLPVLLLLVSNSKAQSSGGVFDITKYGAKPNADVTAALASAWKEACASTTP 60

Query: 61  SKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPPHPTGDGLVLLEYIDQLTLSGTGV 120
           SKVL+PKG Y LSQSNLKGPCKS IE Q+EGTLQAP  P GDGL+LLEY+DQLT+SGTGV
Sbjct: 61  SKVLIPKGSYGLSQSNLKGPCKSAIELQIEGTLQAPADPKGDGLLLLEYVDQLTVSGTGV 120

Query: 121 FDGQGKAGWEKNDCHQKKICTKLPM----------------------------------- 180
            DGQGKAGWEKNDCH KK CTKLPM                                   
Sbjct: 121 LDGQGKAGWEKNDCHLKKECTKLPMNLKLNFVTNSIVKDITSLDSKNYHINLLGCKNLTF 180

Query: 181 ------------------------INILDTKIATGDDCVSVGDSNKQITITNVTCGPGHG 240
                                   INIL+T IATGDDC+SVGD+NKQ+ I++VTCGPGHG
Sbjct: 181 DHVTITAPGNSPNTDGIHVSSSEQINILNTNIATGDDCISVGDTNKQVVISDVTCGPGHG 240

Query: 241 ISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVS 300
           ISIGSLGKY+KEK+VVGV VKKCK+  TTNGVRIKTWPDSA AF A+DMHFEDIEMVNVS
Sbjct: 241 ISIGSLGKYSKEKEVVGVTVKKCKLTDTTNGVRIKTWPDSAVAFPASDMHFEDIEMVNVS 300

Query: 301 NPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVAD 343
           NPVIIDQEYCPWNQCNRKV SKIKIS VSFKNIRGTSATPVAVK VCS+S PCEGVEVAD
Sbjct: 301 NPVIIDQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKIVCSQSNPCEGVEVAD 360

BLAST of Spg006103 vs. ExPASy TrEMBL
Match: A0A5D3DYI3 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00050 PE=3 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.6e-154
Identity = 280/404 (69.31%), Postives = 308/404 (76.24%), Query Frame = 0

Query: 1   MGFKTNFVALLPLLLLLVSTANGQ---YVFDVTKYGAKPNADITQALANAWKEACASTTP 60
           MG  +NFVALLP+LLLLVS +  Q    VFD+TKYGAKPNAD+T AL +AWKEACASTTP
Sbjct: 1   MGLISNFVALLPVLLLLVSNSKAQSSGSVFDITKYGAKPNADVTAALVSAWKEACASTTP 60

Query: 61  SKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPPHPTGDGLVLLEYIDQLTLSGTGV 120
           SKVL+PKG Y LSQSNLKGPCKS IE Q+EGTLQAP  P GDGL+LLEY+DQLT+SGTGV
Sbjct: 61  SKVLIPKGSYGLSQSNLKGPCKSAIELQIEGTLQAPADPKGDGLLLLEYVDQLTVSGTGV 120

Query: 121 FDGQGKAGWEKNDCHQKKICTKLPM----------------------------------- 180
            DGQGKAGWEKNDCH KK CTKLPM                                   
Sbjct: 121 LDGQGKAGWEKNDCHLKKECTKLPMNLKLNFVTNSIVKDITSLDSKNYHINLLGCKNLTF 180

Query: 181 ------------------------INILDTKIATGDDCVSVGDSNKQITITNVTCGPGHG 240
                                   INIL+T IATGDDC+SVGD+NKQ+ I++VTCGPGHG
Sbjct: 181 DHVTITAPGNSPNTDGIHVSSSEQINILNTNIATGDDCISVGDTNKQVVISDVTCGPGHG 240

Query: 241 ISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVS 300
           ISIGSLGKY+KEK+VVGV VKKCK+  TTNGVRIKTWPDSA AF A+DMHFEDIEMVNVS
Sbjct: 241 ISIGSLGKYSKEKEVVGVTVKKCKLTDTTNGVRIKTWPDSAVAFPASDMHFEDIEMVNVS 300

Query: 301 NPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVAD 343
           NPVIIDQEYCPWNQCNRKV SKIKIS VSFKNIRGTSATPVAVK VCS+S PCEGVEVAD
Sbjct: 301 NPVIIDQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKIVCSQSNPCEGVEVAD 360

BLAST of Spg006103 vs. ExPASy TrEMBL
Match: A0A5A7TR51 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G00990 PE=3 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.7e-154
Identity = 280/404 (69.31%), Postives = 308/404 (76.24%), Query Frame = 0

Query: 1   MGFKTNFVALLPLLLLLVSTANGQY---VFDVTKYGAKPNADITQALANAWKEACASTTP 60
           MG  +NFVALL +LLLLVS +  Q    VFD+TKYGAKPNAD+T ALA+AWKEACASTTP
Sbjct: 1   MGLISNFVALLSVLLLLVSNSKAQSSGGVFDITKYGAKPNADVTAALASAWKEACASTTP 60

Query: 61  SKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPPHPTGDGLVLLEYIDQLTLSGTGV 120
           SKVL+PKG Y LSQSNLKGPCKS IE Q+EGTLQAP  P GDGL+LLEY+DQLT+SGTGV
Sbjct: 61  SKVLIPKGSYGLSQSNLKGPCKSAIELQIEGTLQAPADPKGDGLLLLEYVDQLTVSGTGV 120

Query: 121 FDGQGKAGWEKNDCHQKKICTKLPM----------------------------------- 180
            DGQGKAGWEKNDCH KK CTKLPM                                   
Sbjct: 121 LDGQGKAGWEKNDCHLKKECTKLPMNLKLNFVTNSIVKDITSLDSKNYHINLLGCKNLTF 180

Query: 181 ------------------------INILDTKIATGDDCVSVGDSNKQITITNVTCGPGHG 240
                                   INIL+T IATGDDC+SVGD+NKQ+ I++VTCGPGHG
Sbjct: 181 DHVTITAPGNSPNTDGIHVSSSEQINILNTNIATGDDCISVGDTNKQVVISDVTCGPGHG 240

Query: 241 ISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVS 300
           ISIGSLGKY+KEK+VVGV VKKCK+  TTNGVRIKTWPDSA AF A+DMHFEDIEMVNVS
Sbjct: 241 ISIGSLGKYSKEKEVVGVTVKKCKLTDTTNGVRIKTWPDSAVAFPASDMHFEDIEMVNVS 300

Query: 301 NPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVAD 343
           NPVIIDQEYCPWNQCNRKV SKIKIS VSFKNIRGTSATPVAVK VCS+S PCEGVEVAD
Sbjct: 301 NPVIIDQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKIVCSQSNPCEGVEVAD 360

BLAST of Spg006103 vs. ExPASy TrEMBL
Match: A0A5D3BWP4 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold280G00550 PE=3 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.8e-153
Identity = 278/404 (68.81%), Postives = 306/404 (75.74%), Query Frame = 0

Query: 1   MGFKTNFVALLPLLLLLVSTANGQ---YVFDVTKYGAKPNADITQALANAWKEACASTTP 60
           MG  +NFVALL +LLLLVS    Q    V D+TK+GAKPNAD+T ALA+AWKEACASTTP
Sbjct: 1   MGLISNFVALLHVLLLLVSNCKAQSSGVVIDITKFGAKPNADVTAALASAWKEACASTTP 60

Query: 61  SKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPPHPTGDGLVLLEYIDQLTLSGTGV 120
           SKVL+PKG Y LSQSNLKGPCKS IE Q+EGTLQAP  P GDGL+LLEY+DQLT+SGTGV
Sbjct: 61  SKVLIPKGSYGLSQSNLKGPCKSAIELQIEGTLQAPADPKGDGLLLLEYVDQLTVSGTGV 120

Query: 121 FDGQGKAGWEKNDCHQKKICTKLPM----------------------------------- 180
            DGQGKAGWEKNDCH KK CTKLPM                                   
Sbjct: 121 LDGQGKAGWEKNDCHLKKECTKLPMNLKLNFVTNSIVKDITSLDSKNYHINLLGCKNLTF 180

Query: 181 ------------------------INILDTKIATGDDCVSVGDSNKQITITNVTCGPGHG 240
                                   INIL+T IATGDDC+SVGD+NKQ+ I++VTCGPGHG
Sbjct: 181 DHVTITAPGNSPNTDGIHVSSSEQINILNTNIATGDDCISVGDTNKQVVISDVTCGPGHG 240

Query: 241 ISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVS 300
           ISIGSLGKY+KEK+VVGV VKKCK+  TTNGVRIKTWPDSA AF A+DMHFEDIEMVNVS
Sbjct: 241 ISIGSLGKYSKEKEVVGVTVKKCKLTDTTNGVRIKTWPDSAVAFPASDMHFEDIEMVNVS 300

Query: 301 NPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVAD 343
           NPVIIDQEYCPWNQCNRKV SKIKIS VSFKNIRGTSATPVAVK VCS+S PCEGVEVAD
Sbjct: 301 NPVIIDQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKIVCSQSNPCEGVEVAD 360

BLAST of Spg006103 vs. ExPASy TrEMBL
Match: A0A5A7ULW5 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G006240 PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 2.0e-152
Identity = 277/404 (68.56%), Postives = 305/404 (75.50%), Query Frame = 0

Query: 1   MGFKTNFVALLPLLLLLVSTANGQ---YVFDVTKYGAKPNADITQALANAWKEACASTTP 60
           MG ++NFVALL L LLLVS +  Q    VFDVT +GAKP+ADIT AL +AWKE CAS TP
Sbjct: 1   MGLRSNFVALLLLFLLLVSNSKAQSSGAVFDVTSFGAKPDADITGALVSAWKEGCASITP 60

Query: 61  SKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPPHPTGDGLVLLEYIDQLTLSGTGV 120
           SKVL+PKG Y LSQSNLKGPCKS IE Q+EGTLQAP  P GDGL++LEY+D+LT+SG GV
Sbjct: 61  SKVLIPKGSYGLSQSNLKGPCKSSIELQIEGTLQAPSDPKGDGLLILEYVDRLTVSGMGV 120

Query: 121 FDGQGKAGWEKNDCHQKKICTKLPM----------------------------------- 180
            DGQGK GWEKNDCH KKICTKLPM                                   
Sbjct: 121 LDGQGKEGWEKNDCHLKKICTKLPMNLKLNFITNSIVKDITSLDSKNYHINLLGCKNLTF 180

Query: 181 ------------------------INILDTKIATGDDCVSVGDSNKQITITNVTCGPGHG 240
                                   INIL+T IATGDDC+SVGD+NKQI I++VTCGPGHG
Sbjct: 181 DHVTITAPGDSPNTDGIHVSSSEEINILNTNIATGDDCISVGDTNKQIVISDVTCGPGHG 240

Query: 241 ISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVS 300
           ISIGSLGKY+KEK+VVGV VKKCK+ STTNGVRIKTWPDS G F A+DMHFEDIEMVNVS
Sbjct: 241 ISIGSLGKYSKEKEVVGVTVKKCKLTSTTNGVRIKTWPDSVGTFPASDMHFEDIEMVNVS 300

Query: 301 NPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVAD 343
           NPVIIDQEYCPWNQCNRKV SKIKISKVSFKNIRGTSATPVAVK VCSKS PCE VEVAD
Sbjct: 301 NPVIIDQEYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPVAVKIVCSKSNPCEEVEVAD 360

BLAST of Spg006103 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 321.2 bits (822), Expect = 9.8e-88
Identity = 165/388 (42.53%), Postives = 229/388 (59.02%), Query Frame = 0

Query: 8   VALLPLLLLLVSTANGQYVFDVTKYGAKPNADITQALANAWKEACASTTPSKVLVPKGVY 67
           V+ + ++ LL  +AN + VF +   G+   +DITQAL  A+  AC S++PSKV++PKG +
Sbjct: 7   VSTIFIICLLGISANAE-VFTI---GSSSGSDITQALLKAFTSACQSSSPSKVVIPKGEF 66

Query: 68  QLSQSNLKGPCKSPIEFQVEGTLQAPPHPT--GDGLVLLEYIDQLTLSGTGVFDGQGKAG 127
           +L +  ++GPCK+PIE  ++GT++A  +     +  V+   ID   L+G G FDG+G A 
Sbjct: 67  KLGEIEMRGPCKAPIEVTLQGTVKADGNAIQGKEKWVVFGNIDGFKLNGGGAFDGEGNAA 126

Query: 128 WEKNDCHQKKICTKLPM------------------------------------------- 187
           W  N+CH+   C KLP+                                           
Sbjct: 127 WRVNNCHKTFECKKLPISIRFDFILNSEIRDISSIDAKNFHINVLGAKNMTMNNIKIVAP 186

Query: 188 ----------------INILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISIGSLGK 247
                           + IL++ I+TGDDC+SVGD  K + +  VTCGPGHGIS+GSLG+
Sbjct: 187 EDSPNTDGIHLGRSDGVKILNSFISTGDDCISVGDGMKNLHVEKVTCGPGHGISVGSLGR 246

Query: 248 YTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPVIIDQE 307
           Y  E+DV G+ V  C +  T NG+RIKTWP +A + +A+D+HFEDI + +VSNP++IDQE
Sbjct: 247 YGHEQDVSGIKVINCTLQETDNGLRIKTWPSAACSTTASDIHFEDIILKDVSNPILIDQE 306

Query: 308 YCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVADIDLTYNGT 335
           YCPWNQCN++ AS IK+  +SFKNIRGTS    AVK +CSK  PC+ VE+ DID+ YNG 
Sbjct: 307 YCPWNQCNKQKASTIKLVNISFKNIRGTSGNKDAVKLLCSKGYPCQNVEIGDIDIKYNGA 366

BLAST of Spg006103 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 312.0 bits (798), Expect = 5.9e-85
Identity = 167/397 (42.07%), Postives = 226/397 (56.93%), Query Frame = 0

Query: 1   MGFKTNFVALLPLLLLLVSTANGQYVFDVTKYGAKPNADITQALANAWKEACASTTPSKV 60
           MG      A+L ++ LL  +AN + VF+V   G  P +DITQAL  A+  AC + T SKV
Sbjct: 1   MGSYLGISAVLFVISLLGFSANAE-VFNV---GGSPGSDITQALLKAFTSACQAPTASKV 60

Query: 61  LVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPPHPT--GDGLVLLEYIDQLTLSGTGVF 120
           ++ KG ++L +  + GPCK+P+E  ++GTL+A        +  V+   I+   L+G G+F
Sbjct: 61  VITKGEFKLGEIEMTGPCKAPVEINLQGTLKADGKAIQGKERWVVFLRINGFKLNGGGIF 120

Query: 121 DGQGKAGWEKNDCHQKKICTKLPM------------------------------------ 180
           DG+G A W  N+CH+   C KLP+                                    
Sbjct: 121 DGEGNAAWRVNNCHKTFECKKLPISIRFDFVENAEIRDISSIDAKNFHINVLGAKNMTFD 180

Query: 181 -----------------------INILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGI 240
                                  + IL++KIATGDDC+SVGD  K + + NV CGPGHGI
Sbjct: 181 NVKVIAPAESPNTDGIHLGRSEGVKILNSKIATGDDCISVGDGMKNLHVENVMCGPGHGI 240

Query: 241 SIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSN 300
           S+GSLG+Y  E+DV G+ V  C +  T NG+RIKTWP +A A +A+ +HFE+I + NVSN
Sbjct: 241 SVGSLGRYVHEQDVTGITVVNCTLQGTDNGLRIKTWPSAACATTASGIHFENIILNNVSN 300

Query: 301 PVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVADI 336
           P++IDQEYCPWNQCN++  S IK+  +SFKNIRGTS    AVK +CSK+ PC  VE+ +I
Sbjct: 301 PILIDQEYCPWNQCNKQKPSTIKLVDISFKNIRGTSGNKDAVKLLCSKAHPCANVEIGNI 360

BLAST of Spg006103 vs. TAIR 10
Match: AT3G07830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 305.8 bits (782), Expect = 4.3e-83
Identity = 156/393 (39.69%), Postives = 224/393 (57.00%), Query Frame = 0

Query: 8   VALLPLLLLLVSTANGQYVFDVTKYGAKPNADITQALANAWKEACASTTPSKVLVPKGVY 67
           ++ + ++ LL  +AN   V  +T   + P +DITQAL  A+  AC S TP KV++PKG +
Sbjct: 7   ISTIFVICLLGFSANAHEVLRLT---SSPGSDITQALLRAFTTACQSPTPRKVVIPKGQF 66

Query: 68  QLSQSNLKGPCKSPIEFQVEGTLQAPPHPT--GDGLVLLEYIDQLTLSGTGVFDGQGKAG 127
           +L +  + GPCKSP+E  + GT+ A  +     +  V+ + +D   L+G G FDG+G A 
Sbjct: 67  KLGEIMMSGPCKSPVEITLLGTVLADGNSIHGKEKWVVFQRMDGFRLNGGGTFDGEGNAA 126

Query: 128 WEKNDCHQKKICTKLPM------------------------------------------- 187
           W  N+CH+   C KLP+                                           
Sbjct: 127 WRVNNCHKTFECKKLPISIRFDFVTNAEIRDISSIDAKNFHINVIGAKNMTFDNVKIMAP 186

Query: 188 ----------------INILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISIGSLGK 247
                           ++I++++I+TGDDCVSVGD    + + NV CGPGHGIS+GSLG+
Sbjct: 187 AESPNTDGIHLGRSVGVSIINSRISTGDDCVSVGDGMVNLLVKNVVCGPGHGISVGSLGR 246

Query: 248 YTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPVIIDQE 307
           Y  E+DV G+ V  C +  T NG+RIKTWP +A + +A+++HFE+I + NVSNP++IDQE
Sbjct: 247 YGHEQDVSGIRVINCTLQETDNGLRIKTWPSAACSTTASNIHFENIILRNVSNPILIDQE 306

Query: 308 YCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVADIDLTYNGT 340
           YCPWNQCN++ +S IK++ +SF+ IRGTS    AVK +CSK  PCE V+V DI++ Y G 
Sbjct: 307 YCPWNQCNKQKSSSIKLANISFRRIRGTSGNKDAVKLLCSKGYPCENVQVGDINIQYTGA 366

BLAST of Spg006103 vs. TAIR 10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 304.3 bits (778), Expect = 1.2e-82
Identity = 157/378 (41.53%), Postives = 216/378 (57.14%), Query Frame = 0

Query: 16  LLVSTANGQYVFDVTKYGAKPNADITQALANAWKEACASTTPSKVLVPKGVYQLSQSNLK 75
           LL  +AN + +F++    + P +DIT AL  A+ EAC   T S V++PKG Y+L +  + 
Sbjct: 16  LLGLSANAK-IFNI---NSPPGSDITNALLKAFNEACQFPTKSTVMIPKGEYKLGEIVMM 75

Query: 76  GPCKSPIEFQVEGTLQAPPHPTG-DGLVLLEYIDQLTLSGTGVFDGQGKAGWEKNDCHQK 135
           GPCK+PI   + GT++A  +  G +  V    I+   L+G GVFDG+G A W  N+CH+ 
Sbjct: 76  GPCKAPIRIALLGTVKADGNANGKEKWVAFRNINGFKLNGGGVFDGEGNAAWRVNNCHKT 135

Query: 136 KICTKLPM---------------------------------------------------- 195
             C KLP+                                                    
Sbjct: 136 FNCKKLPISIRFDFVTDAKIRGITSLDAKHFHINVIGAKNVTFEDVKIIAPAESPNTDGI 195

Query: 196 -------INILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISIGSLGKYTKEKDVVG 255
                  I I+++ I+TGDDCVSVGD  K + +  VTCGPGHGISIGSLG+Y+ E++V G
Sbjct: 196 HVGRSDGIKIINSFISTGDDCVSVGDGMKNLLVERVTCGPGHGISIGSLGRYSHEENVSG 255

Query: 256 VFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPVIIDQEYCPWNQCNR 315
           + +  C +  T NG+RIKTWP +A   +A+D+HFE+I + NVSNP++IDQEYCPWNQCN+
Sbjct: 256 IKIINCTLQETDNGLRIKTWPSAACTTTASDIHFENILLKNVSNPILIDQEYCPWNQCNK 315

Query: 316 KVASKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVADIDLTYNGTQGPIKSQCS 334
           +  S IK++ +SFK IRGTS    AVK +CSK  PC+ VEV D+++ Y G  GP   QCS
Sbjct: 316 QKPSTIKLANISFKKIRGTSGNKDAVKLLCSKGYPCQNVEVGDVNIQYTGADGPATFQCS 375

BLAST of Spg006103 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 283.1 bits (723), Expect = 3.0e-76
Identity = 157/376 (41.76%), Postives = 204/376 (54.26%), Query Frame = 0

Query: 28  DVTKYGAKPN--ADITQALANAWKEACASTTPSKVLVPKGVYQLSQSNLKGPCKSPIEFQ 87
           DV   GAK +   D + A A AWKEACA+   S + VPKG Y +     KGPCK P+  +
Sbjct: 72  DVKASGAKGDGKTDDSAAFAAAWKEACAA--GSTITVPKGEYLVESLEFKGPCKGPVTLE 131

Query: 88  VEGTLQAP-------PHPTGDGLVLLEYIDQLTLSGT-GVFDGQGKAGWEKNDCHQKKIC 147
           + G  +AP       PH    G +  E +   TL+G   +FDGQG   W+ NDC +   C
Sbjct: 132 LNGNFKAPATVKTTKPHA---GWIDFENLADFTLNGNKAIFDGQGSLAWKANDCAKTGKC 191

Query: 148 TKLPM------------------------------------------------------- 207
             LP+                                                       
Sbjct: 192 NSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIG 251

Query: 208 ----INILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISIGSLGKYTKEKDVVGVFV 267
               +N++  KI TGDDCVS+GD  + + + NV CGPGHGISIGSLG+Y  E+ V GV V
Sbjct: 252 RSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTV 311

Query: 268 KKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKVA 327
           +KC I +T NGVRIKTWP S     A+++ FEDI M NVS PV+IDQEYCP+  C   V 
Sbjct: 312 RKCLIKNTDNGVRIKTWPGSPPGI-ASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVP 371

Query: 328 SKIKISKVSFKNIRGTSATPVAVKFVCSKSLPCEGVEVADIDLTYNGTQGPIKSQCSNVK 335
           SK+K+S V+ KNI+GTSAT VAVK +CSK +PC  + ++DI+L +NG +GP  S CSN+K
Sbjct: 372 SKVKLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIK 431

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011652893.24.6e-15670.20exopolygalacturonase [Cucumis sativus] >KGN64588.2 hypothetical protein Csa_0140... [more]
KAA0045593.13.3e-15469.31exopolygalacturonase-like [Cucumis melo var. makuwa] >TYK28803.1 exopolygalactur... [more]
KAA0045608.13.3e-15469.31exopolygalacturonase-like [Cucumis melo var. makuwa] >TYK02648.1 exopolygalactur... [more]
XP_031744817.15.7e-15470.38exopolygalacturonase-like [Cucumis sativus] >KAE8645715.1 hypothetical protein C... [more]
XP_031744814.15.7e-15470.38exopolygalacturonase-like [Cucumis sativus] >XP_031744815.1 LOW QUALITY PROTEIN:... [more]
Match NameE-valueIdentityDescription
Q059673.9e-9746.62Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1[more]
P245481.5e-9349.30Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1[more]
Q403126.4e-9245.60Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1[more]
Q397861.7e-8943.51Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1[more]
Q397663.9e-8943.51Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7TRZ01.6e-15469.31Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A5D3DYI31.6e-15469.31Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A5A7TR514.7e-15469.31Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A5D3BWP48.8e-15368.81Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A5A7ULW52.0e-15268.56Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
Match NameE-valueIdentityDescription
AT3G07820.19.8e-8842.53Pectin lyase-like superfamily protein [more]
AT3G07840.15.9e-8542.07Pectin lyase-like superfamily protein [more]
AT3G07830.14.3e-8339.69Pectin lyase-like superfamily protein [more]
AT5G48140.11.2e-8241.53Pectin lyase-like superfamily protein [more]
AT3G14040.13.0e-7641.76Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 16..142
e-value: 1.6E-21
score: 78.7
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 143..318
e-value: 1.3E-53
score: 184.0
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 52..141
e-value: 1.4E-13
score: 50.6
coord: 143..319
e-value: 9.5E-58
score: 195.8
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 170..183
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 143..334
coord: 6..137
NoneNo IPR availablePANTHERPTHR31375:SF248POLYGALACTURONASEcoord: 143..334
coord: 6..137
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 14..332

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg006103.1Spg006103.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity