Spg004122 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg004122 vs. NCBI nr
Match: XP_038888073.1 (ent-kaur-16-ene synthase, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 1231.5 bits (3185), Expect = 0.0e+00
Identity = 624/798 (78.20%), Postives = 658/798 (82.46%), Query Frame = 0

Query: 20  SASLFPGLDVETKTKTGALRFEDTKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 79
           SASLFP LDV+TKTKTGALR E+TKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL
Sbjct: 72  SASLFPELDVDTKTKTGALRLEETKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 131

Query: 80  FPECINWVLDNQHSDGSWGILHNHQLLMKATLLSTLACVLTLKRWNIGHDHMSKALNFIK 139
           FPECINWVLDNQH DGSWGILH+HQLLMKATLLSTLACVLTLKRWNIG DHMSKAL+FIK
Sbjct: 132 FPECINWVLDNQHPDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGQDHMSKALSFIK 191

Query: 140 SNIASATDEKQHSPVGFDIIFPGMIEYAKDLNLNLPMTPMNVDALVQKKELELRRSNCEG 199
           SNIASATDEKQ SPVGFDIIFPGMIEYAKDLNLNLP+ PM VDALVQKKELELRRSN EG
Sbjct: 192 SNIASATDEKQRSPVGFDIIFPGMIEYAKDLNLNLPLAPMKVDALVQKKELELRRSNSEG 251

Query: 200 GKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSLLQK 259
           GKAYLAYVSEGI  LQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRS+LQK
Sbjct: 252 GKAYLAYVSEGIENLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSVLQK 311

Query: 260 FDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQGEENIFLDAST 319
           FD SVPTIYPLDIYARLHMVDSLQK GI RHFKEEIRSVLDETYRCWMQGEENIFLDAST
Sbjct: 312 FDDSVPTIYPLDIYARLHMVDSLQKFGIGRHFKEEIRSVLDETYRCWMQGEENIFLDAST 371

Query: 320 CAMAFRMLRVEGYDVSSDQLTQFSEDLFSNCLGGYLKDFGASLELFKASQIIIYPEESVL 379
           CAMAFR+LRVEGYDVSSD+LTQFSE LF NCLGG+LKDFGAS+ELFKASQIIIYP+ES+L
Sbjct: 372 CAMAFRLLRVEGYDVSSDKLTQFSEVLFPNCLGGHLKDFGASMELFKASQIIIYPDESIL 431

Query: 380 ENINSWTSRFLNHGLSSGSVCSDRTDGFIKQEAVSALEFTYKASLERLSNKRAMESYNGN 439
           EN+NSWTSRFLNHGLS G V SDRTD  +KQEAV+ALEF Y A+LERL NKRA+ESY+G+
Sbjct: 432 ENVNSWTSRFLNHGLSGGLVRSDRTDRLVKQEAVNALEFPYNATLERLLNKRALESYSGD 491

Query: 440 IVRISKTTYACLNFGHQDFLEFAVKDFNTLQCIHRKELKEL------------------- 499
           IVRISKTTYACLNFGHQDFLE AV+DFNTLQCIHRKELKEL                   
Sbjct: 492 IVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQKL 551

Query: 500 -----------------------------------------------------ERWDANG 559
                                                                E+WDANG
Sbjct: 552 AYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELLNLIQLVEKWDANG 611

Query: 560 GEDYCSKEVEIIFLALHSTICEIGKRALAWQGRSVTRNVIDIWLALLESMRKEAEWLRNR 619
              YCSKEVEIIFLALHST+C+IG+RAL+WQGRSVTRNVIDIWLALLESMRKEAEWL+N+
Sbjct: 612 ETGYCSKEVEIIFLALHSTVCDIGRRALSWQGRSVTRNVIDIWLALLESMRKEAEWLKNK 671

Query: 620 VAPSMDEYMENGYVSFALGPIVLPALYFVGPKLSEEVIGNCEYQKLFKLMSTCGRLLNDT 679
           V PSMDEYMENGYVSFALGPIVLP LYFVGPKL EEVIGNCEY+KLFKLMSTCGRLLNDT
Sbjct: 672 VVPSMDEYMENGYVSFALGPIVLPTLYFVGPKLPEEVIGNCEYKKLFKLMSTCGRLLNDT 731

Query: 680 RTFDVSTETSLHDLIVFTLRVLSFLYKPLLALLQRESNEGKLNAVSLCMINAGGNFTKEE 739
           RTFD                              RES+EGKLNA+SLC+INAGG  TKEE
Sbjct: 732 RTFD------------------------------RESSEGKLNAMSLCIINAGGKLTKEE 791

Query: 740 AAEVIKGDVERTRRELLRLVLQEKNSTIPRACMDLFWKMSCVVHLFYRKDDGFTSHELMN 746
           A E +KGDVERTRRELLRLVLQE N+TIPRAC DLF KMSCVVHLFYRKDDGFTSHELM 
Sbjct: 792 ATEAMKGDVERTRRELLRLVLQE-NTTIPRACKDLFRKMSCVVHLFYRKDDGFTSHELMK 838

BLAST of Spg004122 vs. NCBI nr
Match: XP_038888074.1 (ent-kaur-16-ene synthase, chloroplastic isoform X3 [Benincasa hispida])

HSP 1 Score: 1226.5 bits (3172), Expect = 0.0e+00
Identity = 624/800 (78.00%), Postives = 658/800 (82.25%), Query Frame = 0

Query: 20  SASLFPGLDVETKTKTGALRFEDTKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 79
           SASLFP LDV+TKTKTGALR E+TKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL
Sbjct: 17  SASLFPELDVDTKTKTGALRLEETKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 76

Query: 80  FPECINWVLDNQHSDGSWGILHNHQLLMKATLLSTLACVLTLKRWNIGHDHMSKALNFIK 139
           FPECINWVLDNQH DGSWGILH+HQLLMKATLLSTLACVLTLKRWNIG DHMSKAL+FIK
Sbjct: 77  FPECINWVLDNQHPDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGQDHMSKALSFIK 136

Query: 140 SNIASATDEKQHSPVGFDIIFPGMIEYAKDLNLNLPMTPMNVDALVQKKELELR--RSNC 199
           SNIASATDEKQ SPVGFDIIFPGMIEYAKDLNLNLP+ PM VDALVQKKELELR  RSN 
Sbjct: 137 SNIASATDEKQRSPVGFDIIFPGMIEYAKDLNLNLPLAPMKVDALVQKKELELRSCRSNS 196

Query: 200 EGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSLL 259
           EGGKAYLAYVSEGI  LQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRS+L
Sbjct: 197 EGGKAYLAYVSEGIENLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSVL 256

Query: 260 QKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQGEENIFLDA 319
           QKFD SVPTIYPLDIYARLHMVDSLQK GI RHFKEEIRSVLDETYRCWMQGEENIFLDA
Sbjct: 257 QKFDDSVPTIYPLDIYARLHMVDSLQKFGIGRHFKEEIRSVLDETYRCWMQGEENIFLDA 316

Query: 320 STCAMAFRMLRVEGYDVSSDQLTQFSEDLFSNCLGGYLKDFGASLELFKASQIIIYPEES 379
           STCAMAFR+LRVEGYDVSSD+LTQFSE LF NCLGG+LKDFGAS+ELFKASQIIIYP+ES
Sbjct: 317 STCAMAFRLLRVEGYDVSSDKLTQFSEVLFPNCLGGHLKDFGASMELFKASQIIIYPDES 376

Query: 380 VLENINSWTSRFLNHGLSSGSVCSDRTDGFIKQEAVSALEFTYKASLERLSNKRAMESYN 439
           +LEN+NSWTSRFLNHGLS G V SDRTD  +KQEAV+ALEF Y A+LERL NKRA+ESY+
Sbjct: 377 ILENVNSWTSRFLNHGLSGGLVRSDRTDRLVKQEAVNALEFPYNATLERLLNKRALESYS 436

Query: 440 GNIVRISKTTYACLNFGHQDFLEFAVKDFNTLQCIHRKELKEL----------------- 499
           G+IVRISKTTYACLNFGHQDFLE AV+DFNTLQCIHRKELKEL                 
Sbjct: 437 GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQ 496

Query: 500 -------------------------------------------------------ERWDA 559
                                                                  E+WDA
Sbjct: 497 KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELLNLIQLVEKWDA 556

Query: 560 NGGEDYCSKEVEIIFLALHSTICEIGKRALAWQGRSVTRNVIDIWLALLESMRKEAEWLR 619
           NG   YCSKEVEIIFLALHST+C+IG+RAL+WQGRSVTRNVIDIWLALLESMRKEAEWL+
Sbjct: 557 NGETGYCSKEVEIIFLALHSTVCDIGRRALSWQGRSVTRNVIDIWLALLESMRKEAEWLK 616

Query: 620 NRVAPSMDEYMENGYVSFALGPIVLPALYFVGPKLSEEVIGNCEYQKLFKLMSTCGRLLN 679
           N+V PSMDEYMENGYVSFALGPIVLP LYFVGPKL EEVIGNCEY+KLFKLMSTCGRLLN
Sbjct: 617 NKVVPSMDEYMENGYVSFALGPIVLPTLYFVGPKLPEEVIGNCEYKKLFKLMSTCGRLLN 676

Query: 680 DTRTFDVSTETSLHDLIVFTLRVLSFLYKPLLALLQRESNEGKLNAVSLCMINAGGNFTK 739
           DTRTFD                              RES+EGKLNA+SLC+INAGG  TK
Sbjct: 677 DTRTFD------------------------------RESSEGKLNAMSLCIINAGGKLTK 736

Query: 740 EEAAEVIKGDVERTRRELLRLVLQEKNSTIPRACMDLFWKMSCVVHLFYRKDDGFTSHEL 746
           EEA E +KGDVERTRRELLRLVLQE N+TIPRAC DLF KMSCVVHLFYRKDDGFTSHEL
Sbjct: 737 EEATEAMKGDVERTRRELLRLVLQE-NTTIPRACKDLFRKMSCVVHLFYRKDDGFTSHEL 785

BLAST of Spg004122 vs. NCBI nr
Match: XP_038888071.1 (ent-kaur-16-ene synthase, chloroplastic isoform X1 [Benincasa hispida] >XP_038888072.1 ent-kaur-16-ene synthase, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1226.5 bits (3172), Expect = 0.0e+00
Identity = 624/800 (78.00%), Postives = 658/800 (82.25%), Query Frame = 0

Query: 20  SASLFPGLDVETKTKTGALRFEDTKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 79
           SASLFP LDV+TKTKTGALR E+TKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL
Sbjct: 72  SASLFPELDVDTKTKTGALRLEETKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 131

Query: 80  FPECINWVLDNQHSDGSWGILHNHQLLMKATLLSTLACVLTLKRWNIGHDHMSKALNFIK 139
           FPECINWVLDNQH DGSWGILH+HQLLMKATLLSTLACVLTLKRWNIG DHMSKAL+FIK
Sbjct: 132 FPECINWVLDNQHPDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGQDHMSKALSFIK 191

Query: 140 SNIASATDEKQHSPVGFDIIFPGMIEYAKDLNLNLPMTPMNVDALVQKKELELR--RSNC 199
           SNIASATDEKQ SPVGFDIIFPGMIEYAKDLNLNLP+ PM VDALVQKKELELR  RSN 
Sbjct: 192 SNIASATDEKQRSPVGFDIIFPGMIEYAKDLNLNLPLAPMKVDALVQKKELELRSCRSNS 251

Query: 200 EGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSLL 259
           EGGKAYLAYVSEGI  LQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRS+L
Sbjct: 252 EGGKAYLAYVSEGIENLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSVL 311

Query: 260 QKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQGEENIFLDA 319
           QKFD SVPTIYPLDIYARLHMVDSLQK GI RHFKEEIRSVLDETYRCWMQGEENIFLDA
Sbjct: 312 QKFDDSVPTIYPLDIYARLHMVDSLQKFGIGRHFKEEIRSVLDETYRCWMQGEENIFLDA 371

Query: 320 STCAMAFRMLRVEGYDVSSDQLTQFSEDLFSNCLGGYLKDFGASLELFKASQIIIYPEES 379
           STCAMAFR+LRVEGYDVSSD+LTQFSE LF NCLGG+LKDFGAS+ELFKASQIIIYP+ES
Sbjct: 372 STCAMAFRLLRVEGYDVSSDKLTQFSEVLFPNCLGGHLKDFGASMELFKASQIIIYPDES 431

Query: 380 VLENINSWTSRFLNHGLSSGSVCSDRTDGFIKQEAVSALEFTYKASLERLSNKRAMESYN 439
           +LEN+NSWTSRFLNHGLS G V SDRTD  +KQEAV+ALEF Y A+LERL NKRA+ESY+
Sbjct: 432 ILENVNSWTSRFLNHGLSGGLVRSDRTDRLVKQEAVNALEFPYNATLERLLNKRALESYS 491

Query: 440 GNIVRISKTTYACLNFGHQDFLEFAVKDFNTLQCIHRKELKEL----------------- 499
           G+IVRISKTTYACLNFGHQDFLE AV+DFNTLQCIHRKELKEL                 
Sbjct: 492 GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQ 551

Query: 500 -------------------------------------------------------ERWDA 559
                                                                  E+WDA
Sbjct: 552 KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELLNLIQLVEKWDA 611

Query: 560 NGGEDYCSKEVEIIFLALHSTICEIGKRALAWQGRSVTRNVIDIWLALLESMRKEAEWLR 619
           NG   YCSKEVEIIFLALHST+C+IG+RAL+WQGRSVTRNVIDIWLALLESMRKEAEWL+
Sbjct: 612 NGETGYCSKEVEIIFLALHSTVCDIGRRALSWQGRSVTRNVIDIWLALLESMRKEAEWLK 671

Query: 620 NRVAPSMDEYMENGYVSFALGPIVLPALYFVGPKLSEEVIGNCEYQKLFKLMSTCGRLLN 679
           N+V PSMDEYMENGYVSFALGPIVLP LYFVGPKL EEVIGNCEY+KLFKLMSTCGRLLN
Sbjct: 672 NKVVPSMDEYMENGYVSFALGPIVLPTLYFVGPKLPEEVIGNCEYKKLFKLMSTCGRLLN 731

Query: 680 DTRTFDVSTETSLHDLIVFTLRVLSFLYKPLLALLQRESNEGKLNAVSLCMINAGGNFTK 739
           DTRTFD                              RES+EGKLNA+SLC+INAGG  TK
Sbjct: 732 DTRTFD------------------------------RESSEGKLNAMSLCIINAGGKLTK 791

Query: 740 EEAAEVIKGDVERTRRELLRLVLQEKNSTIPRACMDLFWKMSCVVHLFYRKDDGFTSHEL 746
           EEA E +KGDVERTRRELLRLVLQE N+TIPRAC DLF KMSCVVHLFYRKDDGFTSHEL
Sbjct: 792 EEATEAMKGDVERTRRELLRLVLQE-NTTIPRACKDLFRKMSCVVHLFYRKDDGFTSHEL 840

BLAST of Spg004122 vs. NCBI nr
Match: XP_016900364.1 (PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 1220.3 bits (3156), Expect = 0.0e+00
Identity = 620/798 (77.69%), Postives = 659/798 (82.58%), Query Frame = 0

Query: 20  SASLFPGLDVETKTKTGALRFEDTKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 79
           SASLFPGLDV+TKTKTGALRFEDTKERI+KLF  VELSVSAYDTAWVAMVPSPNSLNKPL
Sbjct: 17  SASLFPGLDVDTKTKTGALRFEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPNSLNKPL 76

Query: 80  FPECINWVLDNQHSDGSWGILHNHQLLMKATLLSTLACVLTLKRWNIGHDHMSKALNFIK 139
           FPECINWVLD+Q+ DGSWGILH+HQL+MKATLLSTLACVLTLKRWNIGHDHMSKAL+FIK
Sbjct: 77  FPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSKALSFIK 136

Query: 140 SNIASATDEKQHSPVGFDIIFPGMIEYAKDLNLNLPMTPMNVDALVQKKELELRRSNCEG 199
           SNIASATDE Q SPVGFDIIFPGMIEYAKDLNLNLP+  MNVDALVQKKELEL RSN EG
Sbjct: 137 SNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDALVQKKELEL-RSNSEG 196

Query: 200 GKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSLLQK 259
           GKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLF+SPSTTAVAFMHRNDDGCFNYLRS+LQK
Sbjct: 197 GKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFNYLRSILQK 256

Query: 260 FDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQGEENIFLDAST 319
           F SSVP IYPLDIYARLHMVDSLQKLGIDRHFK+EIRSVLDETYRCWMQGEENIFLDAST
Sbjct: 257 FHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKDEIRSVLDETYRCWMQGEENIFLDAST 316

Query: 320 CAMAFRMLRVEGYDVSSDQLTQFSEDLFSNCLGGYLKDFGASLELFKASQIIIYPEESVL 379
           CAMAFRMLRVEGYDVSSDQLTQFSEDLF NCLGG+LKDFGASLELFKASQIIIYP+E +L
Sbjct: 317 CAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGHLKDFGASLELFKASQIIIYPDEFIL 376

Query: 380 ENINSWTSRFLNHGLSSGSVCSDRTDGFIKQEAVSALEFTYKASLERLSNKRAMESYNGN 439
           ENINSWTSRFLNHGLS GSV SDRT+  +KQEAV+A EF Y ++LERLSNKRA+ESY+G+
Sbjct: 377 ENINSWTSRFLNHGLSGGSVHSDRTERLVKQEAVNAFEFPYNSTLERLSNKRALESYSGD 436

Query: 440 IVRISKTTYACLNFGHQDFLEFAVKDFNTLQCIHRKELKEL------------------- 499
           IVRISKTTYACLNFGHQDFLE AV+DFNTLQCIHRKELKEL                   
Sbjct: 437 IVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVIENKLDKLKFARQKL 496

Query: 500 -----------------------------------------------------ERWDANG 559
                                                                E+WDA+G
Sbjct: 497 AYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDASG 556

Query: 560 GEDYCSKEVEIIFLALHSTICEIGKRALAWQGRSVTRNVIDIWLALLESMRKEAEWLRNR 619
              YCSKEVEIIFLALHSTICEIG++AL WQGRSVTRNVIDIWLALLESMRKEAEWL+N+
Sbjct: 557 EAGYCSKEVEIIFLALHSTICEIGEKALPWQGRSVTRNVIDIWLALLESMRKEAEWLKNK 616

Query: 620 VAPSMDEYMENGYVSFALGPIVLPALYFVGPKLSEEVIGNCEYQKLFKLMSTCGRLLNDT 679
           V PS+DEYMENGYVSFALGPIVLP LYFVGPKL EE+IGNCEYQKLFKLMST GRLLNDT
Sbjct: 617 VVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIIGNCEYQKLFKLMSTSGRLLNDT 676

Query: 680 RTFDVSTETSLHDLIVFTLRVLSFLYKPLLALLQRESNEGKLNAVSLCMINAGGNFTKEE 739
           RTFD                              RES+EGKLNA+SL +I+AGG  +KEE
Sbjct: 677 RTFD------------------------------RESSEGKLNALSLYLISAGGKLSKEE 736

Query: 740 AAEVIKGDVERTRRELLRLVLQEKNSTIPRACMDLFWKMSCVVHLFYRKDDGFTSHELMN 746
           A E +KGDV+RTRRELLRLVLQE NSTIPRAC DLFWKMSCVVHLFYRKDDGFTSHELMN
Sbjct: 737 ATEAMKGDVDRTRRELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMN 782

BLAST of Spg004122 vs. NCBI nr
Match: XP_008447073.1 (PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 1219.5 bits (3154), Expect = 0.0e+00
Identity = 620/800 (77.50%), Postives = 659/800 (82.38%), Query Frame = 0

Query: 20  SASLFPGLDVETKTKTGALRFEDTKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 79
           SASLFPGLDV+TKTKTGALRFEDTKERI+KLF  VELSVSAYDTAWVAMVPSPNSLNKPL
Sbjct: 17  SASLFPGLDVDTKTKTGALRFEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPNSLNKPL 76

Query: 80  FPECINWVLDNQHSDGSWGILHNHQLLMKATLLSTLACVLTLKRWNIGHDHMSKALNFIK 139
           FPECINWVLD+Q+ DGSWGILH+HQL+MKATLLSTLACVLTLKRWNIGHDHMSKAL+FIK
Sbjct: 77  FPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSKALSFIK 136

Query: 140 SNIASATDEKQHSPVGFDIIFPGMIEYAKDLNLNLPMTPMNVDALVQKKELELRR--SNC 199
           SNIASATDE Q SPVGFDIIFPGMIEYAKDLNLNLP+  MNVDALVQKKELELR   SN 
Sbjct: 137 SNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDALVQKKELELRSCCSNS 196

Query: 200 EGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSLL 259
           EGGKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLF+SPSTTAVAFMHRNDDGCFNYLRS+L
Sbjct: 197 EGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFNYLRSIL 256

Query: 260 QKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQGEENIFLDA 319
           QKF SSVP IYPLDIYARLHMVDSLQKLGIDRHFK+EIRSVLDETYRCWMQGEENIFLDA
Sbjct: 257 QKFHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKDEIRSVLDETYRCWMQGEENIFLDA 316

Query: 320 STCAMAFRMLRVEGYDVSSDQLTQFSEDLFSNCLGGYLKDFGASLELFKASQIIIYPEES 379
           STCAMAFRMLRVEGYDVSSDQLTQFSEDLF NCLGG+LKDFGASLELFKASQIIIYP+E 
Sbjct: 317 STCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGHLKDFGASLELFKASQIIIYPDEF 376

Query: 380 VLENINSWTSRFLNHGLSSGSVCSDRTDGFIKQEAVSALEFTYKASLERLSNKRAMESYN 439
           +LENINSWTSRFLNHGLS GSV SDRT+  +KQEAV+A EF Y ++LERLSNKRA+ESY+
Sbjct: 377 ILENINSWTSRFLNHGLSGGSVHSDRTERLVKQEAVNAFEFPYNSTLERLSNKRALESYS 436

Query: 440 GNIVRISKTTYACLNFGHQDFLEFAVKDFNTLQCIHRKELKEL----------------- 499
           G+IVRISKTTYACLNFGHQDFLE AV+DFNTLQCIHRKELKEL                 
Sbjct: 437 GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVIENKLDKLKFARQ 496

Query: 500 -------------------------------------------------------ERWDA 559
                                                                  E+WDA
Sbjct: 497 KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDA 556

Query: 560 NGGEDYCSKEVEIIFLALHSTICEIGKRALAWQGRSVTRNVIDIWLALLESMRKEAEWLR 619
           +G   YCSKEVEIIFLALHSTICEIG++AL WQGRSVTRNVIDIWLALLESMRKEAEWL+
Sbjct: 557 SGEAGYCSKEVEIIFLALHSTICEIGEKALPWQGRSVTRNVIDIWLALLESMRKEAEWLK 616

Query: 620 NRVAPSMDEYMENGYVSFALGPIVLPALYFVGPKLSEEVIGNCEYQKLFKLMSTCGRLLN 679
           N+V PS+DEYMENGYVSFALGPIVLP LYFVGPKL EE+IGNCEYQKLFKLMST GRLLN
Sbjct: 617 NKVVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIIGNCEYQKLFKLMSTSGRLLN 676

Query: 680 DTRTFDVSTETSLHDLIVFTLRVLSFLYKPLLALLQRESNEGKLNAVSLCMINAGGNFTK 739
           DTRTFD                              RES+EGKLNA+SL +I+AGG  +K
Sbjct: 677 DTRTFD------------------------------RESSEGKLNALSLYLISAGGKLSK 736

Query: 740 EEAAEVIKGDVERTRRELLRLVLQEKNSTIPRACMDLFWKMSCVVHLFYRKDDGFTSHEL 746
           EEA E +KGDV+RTRRELLRLVLQE NSTIPRAC DLFWKMSCVVHLFYRKDDGFTSHEL
Sbjct: 737 EEATEAMKGDVDRTRRELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHEL 785

BLAST of Spg004122 vs. ExPASy Swiss-Prot
Match: Q39548 (Ent-kaur-16-ene synthase, chloroplastic OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 1133.2 bits (2930), Expect = 0.0e+00
Identity = 579/800 (72.38%), Postives = 631/800 (78.88%), Query Frame = 0

Query: 20  SASLFPGLDVETKTKTGALRFEDTKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 79
           SASLFPG+DV+T TKTGAL FE+TKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLN+PL
Sbjct: 21  SASLFPGVDVDTTTKTGALHFEETKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNQPL 80

Query: 80  FPECINWVLDNQHSDGSWGILHNHQLLMKATLLSTLACVLTLKRWNIGHDHMSKALNFIK 139
           FPECINWVLD+QH+DGSWG+LHN QLLMKA LLSTLACVLTLKRWNIGHDHMSKAL+FIK
Sbjct: 81  FPECINWVLDSQHADGSWGLLHNDQLLMKANLLSTLACVLTLKRWNIGHDHMSKALDFIK 140

Query: 140 SNIASATDEKQHSPVGFDIIFPGMIEYAKDLNLNLPMTPMNVDALVQKKELELR--RSNC 199
           SNIASATDE Q SPVGFDIIFPGMIEYAKDLNLNLP+ P NVDALV+KKELELR  RSN 
Sbjct: 141 SNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLAPTNVDALVRKKELELRSCRSNS 200

Query: 200 EGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSLL 259
           EGGKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLFNSPSTTA AFMHRNDDGCF+YLRSLL
Sbjct: 201 EGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFNSPSTTAAAFMHRNDDGCFDYLRSLL 260

Query: 260 QKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQGEENIFLDA 319
           QKFD SVPTIYPLDIYARLHMVDSLQK GI RHFKEEIRSVLDETYRCWMQGEENIFLDA
Sbjct: 261 QKFDGSVPTIYPLDIYARLHMVDSLQKFGIARHFKEEIRSVLDETYRCWMQGEENIFLDA 320

Query: 320 STCAMAFRMLRVEGYDVSSDQLTQFSEDLFSNCLGGYLKDFGASLELFKASQIIIYPEES 379
           STCAMAFRMLRVEGYDVSSDQLTQFSED+F NCLGGYLKDFGASLEL+KASQII +P+ES
Sbjct: 321 STCAMAFRMLRVEGYDVSSDQLTQFSEDIFPNCLGGYLKDFGASLELYKASQIITHPDES 380

Query: 380 VLENINSWTSRFLNHGLSSGSVCSDRTDGFIKQEAVSALEFTYKASLERLSNKRAMESYN 439
           VLENINSWTSRFL HGLSS SV SDRTD  +KQEAV+ALEF Y A+LERL +KRAMESY+
Sbjct: 381 VLENINSWTSRFLKHGLSSDSVWSDRTDSVVKQEAVNALEFPYNATLERLISKRAMESYS 440

Query: 440 GNIVRISKTTYACLNFGHQDFLEFAVKDFNTLQCIHRKELKELERW-------------- 499
           G+IVRISK+ YACLNFGHQDFLE AV+DFNTLQ IH KEL+EL+RW              
Sbjct: 441 GDIVRISKSPYACLNFGHQDFLELAVEDFNTLQRIHLKELEELQRWVVENKLDELKFFRL 500

Query: 500 ---------------------------------------DANGGED-------------- 559
                                                  D  G E+              
Sbjct: 501 HLGYCYFAAAATLTDPELHDARIAWAQNGVLTTVVDDFYDGGGSEEELDNLIELVEKWDP 560

Query: 560 -----YCSKEVEIIFLALHSTICEIGKRALAWQGRSVTRNVIDIWLALLESMRKEAEWLR 619
                YCSK+VEI+FLALHST+CEIG+RAL WQGRSV RNVID WLALL+ MRKEAEW  
Sbjct: 561 DGEVGYCSKDVEIVFLALHSTVCEIGRRALVWQGRSVMRNVIDGWLALLKVMRKEAEWST 620

Query: 620 NRVAPSMDEYMENGYVSFALGPIVLPALYFVGPKLSEEVIGNCEYQKLFKLMSTCGRLLN 679
           N+V PSM EYME  +VSFALGPI+LP L+FVGPKLSEE+IG+CEYQKL+KLMST GRL N
Sbjct: 621 NKVVPSMGEYMEQAHVSFALGPIILPMLFFVGPKLSEEMIGSCEYQKLYKLMSTAGRLKN 680

Query: 680 DTRTFDVSTETSLHDLIVFTLRVLSFLYKPLLALLQRESNEGKLNAVSLCMINAGGNFTK 739
           D R++D                              RE  EGKLN +SL MI+ GGN TK
Sbjct: 681 DIRSYD------------------------------RECKEGKLNILSLWMIDGGGNVTK 740

Query: 740 EEAAEVIKGDVERTRRELLRLVLQEKNSTIPRACMDLFWKMSCVVHLFYRKDDGFTSHEL 746
           EEA E IKGD ER  RELL LVLQE N+TIPRAC DLFWK+  +V+LFY +DDG+TS+ L
Sbjct: 741 EEAIEAIKGDFERAIRELLGLVLQE-NTTIPRACKDLFWKLMSIVNLFYMEDDGYTSNRL 789

BLAST of Spg004122 vs. ExPASy Swiss-Prot
Match: A0A2K9RFY0 (Ent-kaurene synthase TSP4, chloroplastic OS=Vitex agnus-castus OX=54477 GN=TPS4 PE=1 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 9.3e-205
Identity = 378/794 (47.61%), Postives = 494/794 (62.22%), Query Frame = 0

Query: 20  SASLFPGLDVETKTKTGALRFEDTKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 79
           SASL  G+   T  K  +  FE+TKERI  L  K ELSVS YDTAWVAMVPSPNS  +P 
Sbjct: 25  SASLDTGVRRVTSAKIASTCFEETKERIADLIHKAELSVSTYDTAWVAMVPSPNSSQEPC 84

Query: 80  FPECINWVLDNQHSDGSWGILHNHQLLMKATLLSTLACVLTLKRWNIGHDHMSKALNFIK 139
           FP+C++W+L NQ  DGSW   H+H LL K  L STLACVL LK+W +G + +++ ++FI+
Sbjct: 85  FPDCLSWLLQNQCCDGSWACPHHHPLLKKDVLCSTLACVLALKKWGVGEEKINRGVHFIE 144

Query: 140 SNIASATDEKQHSPVGFDIIFPGMIEYAKDLNLNLPMTPMNVDALVQKKELELRRSNCEG 199
            N ASA ++ Q SP+GFDIIFP M++YA+DL LNL + P  ++ L+ K+ LEL+R+    
Sbjct: 145 HNFASAMEKCQISPMGFDIIFPAMLDYARDLLLNLRLEPTMLNDLIYKRGLELKRNQNHS 204

Query: 200 G--KAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSLL 259
              +AYLAYV+EG+GKLQD   VM++QR+NGSLFNSPSTTA AF+   +  C  YLRS L
Sbjct: 205 AEREAYLAYVAEGMGKLQDLGSVMKHQRRNGSLFNSPSTTAAAFIAFPNSRCLTYLRSAL 264

Query: 260 QKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQGEENIFLDA 319
           +KF S+VP +YPLDIY +L  VD+L++LGI R+F++EI+ VLDETYRCW+QG E IF+DA
Sbjct: 265 KKFGSAVPAVYPLDIYLQLCTVDNLERLGISRYFQKEIQGVLDETYRCWLQGNEEIFMDA 324

Query: 320 STCAMAFRMLRVEGYDVSSDQLTQFSEDLFSNCLGGYLKDFGASLELFKASQIIIYPEES 379
            TCA+AFR+LR  GY+V+SD +T+  E+ FS+   G +KD   +L L++AS+ I+YP+E 
Sbjct: 325 PTCALAFRVLRKNGYNVTSDPITKLLEECFSSSFCGNIKDINTTLGLYRASEFILYPDER 384

Query: 380 VLENINSWTSRFLNHGLSSGSVCSDRTDGFIKQEAVSALEFTYKASLERLSNKRAMESYN 439
            LE  N      L   LSS  + S +    I  E   ALE+ + A L+R+ N+R +E YN
Sbjct: 385 DLEKQNLMLKNLLEQELSSDFIHSSQLGRNIDAEVKHALEYPFYADLDRIVNRRNIEHYN 444

Query: 440 GNIVRISKTTYACLNFGHQDFLEFAVKDFNTLQCIHRKELKELER--------------- 499
            +  RI KT+Y   NFG++DFL  +VKD+N  Q IHR+EL+ELER               
Sbjct: 445 FDNTRILKTSYCSPNFGNKDFLFLSVKDYNECQAIHREELRELERWVIENRLDELRFARQ 504

Query: 500 ---------------------------------------------------------WDA 559
                                                                    WD 
Sbjct: 505 KCAYCYFSAAATLFAPELSNARMSWAKNGVLTTVVDDFFDLGGSVEELKNLIQLVELWDV 564

Query: 560 NGGEDYCSKEVEIIFLALHSTICEIGKRALAWQGRSVTRNVIDIWLALLESMRKEAEWLR 619
           +   +  S+ V+IIF AL  TIC+IG +    Q RS+T ++IDIWL LL SM KE EW R
Sbjct: 565 DVSTECSSQNVQIIFSALKCTICDIGDKGSKLQERSITNHIIDIWLDLLYSMMKETEWAR 624

Query: 620 NRVAPSMDEYMENGYVSFALGPIVLPALYFVGPKLSEEVIGNCEYQKLFKLMSTCGRLLN 679
           ++  P+MDEY+ N YVSFALGPIVLPALY VGPKLSEE++ + EY  L+KLMSTCGRLLN
Sbjct: 625 DKYIPTMDEYISNAYVSFALGPIVLPALYLVGPKLSEEMVHHSEYHNLYKLMSTCGRLLN 684

Query: 680 DTRTFDVSTETSLHDLIVFTLRVLSFLYKPLLALLQRESNEGKLNAVSLCMINAGGNFTK 739
           D R                                +RE  EGKLNA+ L +IN GG  TK
Sbjct: 685 DIRG------------------------------CERELKEGKLNAIPLYIINNGGEITK 744

BLAST of Spg004122 vs. ExPASy Swiss-Prot
Match: A0A1Z3GC64 (Ent-kaurene synthase 5, chloroplastic OS=Isodon rubescens OX=587669 GN=KSL5 PE=1 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 4.1e-200
Identity = 370/800 (46.25%), Postives = 495/800 (61.88%), Query Frame = 0

Query: 20  SASLFPGLDVETKTKTGALR--FEDTKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNK 79
           SASL  GL   T  +  ++   FE+TK RI KLF K ELSVS YDTAWVAMVPSP S  +
Sbjct: 24  SASLDIGLQRATSPQNASVATCFEETKGRIAKLFHKNELSVSTYDTAWVAMVPSPTSSEE 83

Query: 80  PLFPECINWVLDNQHSDGSWGILHNHQLLMKATLLSTLACVLTLKRWNIGHDHMSKALNF 139
           P FP C+NW+L+NQ  DGSW   H+H +L K  L STLAC+L LK+W +G + +++ L+F
Sbjct: 84  PCFPACLNWLLENQCHDGSWARPHHHHMLKKDVLSSTLACILALKKWGVGEEQINRGLHF 143

Query: 140 IKSNIASATDEKQHSPVGFDIIFPGMIEYAKDLNLNLPMTPMNVDALVQKKELELRR--- 199
           ++ N ASAT++ Q +P+GFDIIFP M++ A+ L+LNL + P  ++ L+ K++LEL+R   
Sbjct: 144 VELNFASATEKGQITPMGFDIIFPAMLDNARGLSLNLQLEPTTLNDLIYKRDLELKRCNQ 203

Query: 200 SNCEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLR 259
           SN    + Y A+++EG+GKLQDWE VM+YQRKNGSLFN PSTTA AF    +  C NYLR
Sbjct: 204 SNSAEKEVYWAHIAEGMGKLQDWESVMKYQRKNGSLFNCPSTTAAAFTALRNSDCLNYLR 263

Query: 260 SLLQKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQGEENIF 319
             L+KF S+VP +YPLDIY++L  VD+L++LGI R+F  EI+SVLDETYR W+QG+E IF
Sbjct: 264 LALEKFGSAVPAVYPLDIYSQLCTVDNLERLGISRYFLTEIQSVLDETYRSWLQGDEEIF 323

Query: 320 LDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLFSNCLGGYLKDFGASLELFKASQIIIYP 379
           +DASTCA+AFR LR+ GY+V+SD +T+  ++ FS+   G + D   +LE+++AS++I+YP
Sbjct: 324 MDASTCALAFRTLRMNGYNVTSDPITKILQECFSSSFRGNMTDINTTLEIYRASELILYP 383

Query: 380 EESVLENINSWTSRFLNHGLSS-GSVCSDRTDGFIKQEAVSALEFTYKASLERLSNKRAM 439
           EE  L+  N     FL   LSS G + S +    I  E   A+E+ + A ++R++ ++ +
Sbjct: 384 EERDLDQHNLRLKTFLEQELSSNGFIQSCQLGRNINAEVNQAIEYPFYAIMDRMAKRKNI 443

Query: 440 ESYNGNIVRISKTTYACLNFGHQDFLEFAVKDFNTLQCIHRKELKELER----------- 499
           E+YN +  RI KT+Y   NFG++DFL  +V+DFN  Q IHR+EL+ELER           
Sbjct: 444 ENYNIDNTRILKTSYRSPNFGNKDFLSLSVEDFNRCQVIHREELRELERWVIENRLDELK 503

Query: 500 ------------------------------------------------------------ 559
                                                                       
Sbjct: 504 FARSKAAYCYFSAAATIFSPELSDARMSWAKNGVLTTVVDDFFDVGGSVEELKNLIQLVE 563

Query: 560 -WDANGGEDYCSKEVEIIFLALHSTICEIGKRALAWQGRSVTRNVIDIWLALLESMRKEA 619
            WD +     CS  V+IIF AL  TICEI  +    QGRS+T ++I IWL LL SM KE 
Sbjct: 564 LWDVDVSTQCCSPNVQIIFSALKHTICEIADKGFKLQGRSITDHIISIWLDLLYSMMKET 623

Query: 620 EWLRNRVAPSMDEYMENGYVSFALGPIVLPALYFVGPKLSEEVIGNCEYQKLFKLMSTCG 679
           E   ++  P+MDEYM N YVSFALGPIVLPALY VGPKLSEE++ + EY  LFKLMSTCG
Sbjct: 624 ELGIDKSFPTMDEYMSNAYVSFALGPIVLPALYLVGPKLSEEMVNHSEYHTLFKLMSTCG 683

Query: 680 RLLNDTRTFDVSTETSLHDLIVFTLRVLSFLYKPLLALLQRESNEGKLNAVSLCMINAGG 739
           RLLND R +                              +RE  +GK++AVSL ++N GG
Sbjct: 684 RLLNDIRGY------------------------------ERELKDGKISAVSLYIMNNGG 743

Query: 740 NFTKEEAAEVIKGDVERTRRELLRLVLQEKNSTIPRACMDLFWKMSCVVHLFYRKDDGFT 742
             T E A   ++  +ER RRELLRLVL+E  S +P+AC  LFW M  VVHLFYRKDDGFT
Sbjct: 744 EITTEAAISEMRSWIERDRRELLRLVLEENKSVLPKACKKLFWHMCTVVHLFYRKDDGFT 793

BLAST of Spg004122 vs. ExPASy Swiss-Prot
Match: A0A0U4CDK4 (Ent-kaur-16-ene synthase, chloroplastic OS=Salvia miltiorrhiza OX=226208 GN=KS PE=1 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 4.5e-199
Identity = 363/799 (45.43%), Postives = 488/799 (61.08%), Query Frame = 0

Query: 20  SASLFPGLDVETKTKTGALR--FEDTKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNK 79
           SASL  GL   T  K  ++   FE T+ RI KLF K ELSVS YDTAWVAMVPSP SL +
Sbjct: 27  SASLKSGLHSATSAKIASMPTCFEQTRGRIAKLFHKDELSVSTYDTAWVAMVPSPTSLEE 86

Query: 80  PLFPECINWVLDNQHSDGSWGILHNHQLLMKATLLSTLACVLTLKRWNIGHDHMSKALNF 139
           P FP+C+NW+L+NQ  DGSW   H+H LL K  L STLAC+L LK+W +G + + + L+F
Sbjct: 87  PCFPDCLNWLLENQCHDGSWARPHHHPLLKKDVLSSTLACILALKKWGVGEEQIKRGLHF 146

Query: 140 IKSNIASATDEKQHSPVGFDIIFPGMIEYAKDLNLNLPMTPMNVDALVQKKELELRRSN- 199
           ++ N ASATD+ Q +P+GFDIIFP M++YA+  +LNL + P   + L+ K+++EL+RSN 
Sbjct: 147 LELNFASATDKCQITPMGFDIIFPAMLDYARGFSLNLRLDPTTFNDLMHKRDVELKRSNR 206

Query: 200 --CEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLR 259
                 + Y AY++EG+G+LQ+WE VM+YQR+NGSLFN PSTTA AF+   +  C NYL 
Sbjct: 207 NYSSETETYWAYIAEGMGELQNWESVMKYQRRNGSLFNCPSTTAAAFIALRNSDCLNYLH 266

Query: 260 SLLQKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQGEENIF 319
             L+KF ++V  +YPLDIY++L  VD+L++LGI ++F  EI++VLDETYRCWMQG E IF
Sbjct: 267 LALKKFGNAVSAVYPLDIYSQLCTVDNLERLGISQYFSTEIQNVLDETYRCWMQGNEEIF 326

Query: 320 LDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLFSNCLGGYLKDFGASLELFKASQIIIYP 379
           +DASTCA+AFR LR+ GYDV+SD +TQ  ++ FS+   G + D   +LEL++AS++++YP
Sbjct: 327 MDASTCALAFRTLRLNGYDVTSDPVTQILQECFSSSFRGNMTDINTTLELYRASELVLYP 386

Query: 380 EESVLENINSWTSRFLNHGLSSGSVCSDRTDGFIKQEAVSALEFTYKASLERLSNKRAME 439
           +E  LE  N      L   LSSG + S +    I  E   A+E+ + A ++R++ ++++E
Sbjct: 387 DERDLEKQNLRLKLLLEQELSSGLIQSCQLGRSINVEVNQAIEYPFYAIMDRVAKRKSIE 446

Query: 440 SYNGNIVRISKTTYACLNFGHQDFLEFAVKDFNTLQCIHRKELKELERWDANGGED---- 499
            YN +  RI KT+Y   NF ++DFL  +++DFN  Q  HR+EL ELERW      D    
Sbjct: 447 HYNFDNTRILKTSYCSPNFSNEDFLFLSIEDFNRCQAAHREELGELERWVVENRLDELKF 506

Query: 500 ------YC---------------------------------------------------- 559
                 YC                                                    
Sbjct: 507 ARSKSAYCYFSAAATFFAPELLDARLSWAKNGVLTTVIDDFFDVGGSVEELKNLIQLVEL 566

Query: 560 ----------SKEVEIIFLALHSTICEIGKRALAWQGRSVTRNVIDIWLALLESMRKEAE 619
                     S  V+IIF AL  TICEIG +A   QGR +T ++I IWL LL SM +E E
Sbjct: 567 WDVDISTECYSHNVQIIFSALRRTICEIGDKAFKLQGRCITNHIIAIWLDLLNSMMRETE 626

Query: 620 WLRNRVAPSMDEYMENGYVSFALGPIVLPALYFVGPKLSEEVIGNCEYQKLFKLMSTCGR 679
           W R+   P++DEYM N +VSFALGPIVLPALY VGPKLSEE++ + EY  LFKLMSTCGR
Sbjct: 627 WARDNFVPTIDEYMSNAHVSFALGPIVLPALYLVGPKLSEEMVNHSEYHNLFKLMSTCGR 686

Query: 680 LLNDTRTFDVSTETSLHDLIVFTLRVLSFLYKPLLALLQRESNEGKLNAVSLCMINAGGN 739
           LLND   +                              +RE  +GKLNA+SL +IN GG 
Sbjct: 687 LLNDIHGY------------------------------ERELKDGKLNALSLYIINHGGE 746

Query: 740 FTKEEAAEVIKGDVERTRRELLRLVLQEKNSTIPRACMDLFWKMSCVVHLFYRKDDGFTS 742
            +KE A   +K  +E  RRELLRLVL+ K S +P+ C +LFW M  VVHLFY K DGFTS
Sbjct: 747 VSKEAAIWEMKSWIETQRRELLRLVLEGKKSVLPKPCRELFWHMCSVVHLFYSKGDGFTS 795

BLAST of Spg004122 vs. ExPASy Swiss-Prot
Match: A0A075FBT3 (Ent-kaurene synthase, chloroplastic (Fragment) OS=Marrubium vulgare OX=41230 GN=EKS PE=1 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 7.2e-197
Identity = 364/773 (47.09%), Postives = 475/773 (61.45%), Query Frame = 0

Query: 40  FEDTKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPLFPECINWVLDNQHSDGSWGI 99
           FEDTK RI KL  K ELSVS YDTAWVAMVPSPNS N+P FP+C+ W+L+NQ  DGSW  
Sbjct: 27  FEDTKARIVKLIHKPELSVSTYDTAWVAMVPSPNSSNEPCFPDCLAWLLENQCCDGSWAC 86

Query: 100 LHNHQLLMKATLLSTLACVLTLKRWNIGHDHMSKALNFIKSNIASATDEKQHSPVGFDII 159
            H+H  L K  L STLAC+L LK+W +G + ++K   FI+ N ASAT++ Q SP GFDII
Sbjct: 87  PHHHPFLKKDVLSSTLACILALKKWGVGEEQINKGARFIEVNFASATEKSQISPTGFDII 146

Query: 160 FPGMIEYAKDLNLNLPMTPMNVDALVQKKELELRRSNCEGGKAYLAYVSEGIGKLQDWEM 219
           FP M++YA+DL L+L + P   D L+ +++LEL+R      +AYLAY++EG+GKLQDWE 
Sbjct: 147 FPAMLDYARDLFLDLRLEPTTFDNLIFRRDLELKRCYESHREAYLAYIAEGMGKLQDWES 206

Query: 220 VMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSLLQKFDSSVPTIYPLDIYARLHMV 279
           VM+YQRKNGSLFNSPSTTA AF+   + GC +YL S L+KF ++VP  YPLD+Y+RL  V
Sbjct: 207 VMKYQRKNGSLFNSPSTTAAAFIALPNSGCLSYLHSALKKFGNAVPAAYPLDVYSRLRTV 266

Query: 280 DSLQKLGIDRHFKEEIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQL 339
           D+L+ LGI R+F++EI+ VLDETYR W+QG E IFLDASTCA+AFR LR+ GY+V+SD +
Sbjct: 267 DNLESLGISRYFQKEIQQVLDETYRWWLQGSEEIFLDASTCALAFRTLRMNGYNVTSDAI 326

Query: 340 TQFSEDLFSNCLGGYLKDFGASLELFKASQIIIYPEESVLENINSWTSRFLNHGLSSGSV 399
           T+   D F     G +KD G +LEL++AS+ I+YP+E  LE  N      L   LSS   
Sbjct: 327 TKLLPDSFC----GNMKDIGTTLELYRASEFILYPDEKDLEQQNLRLKDILEQELSS--- 386

Query: 400 CSDRTDGFIKQEAVSALEFTYKASLERLSNKRAMESYNGNIVRISKTTYACLNFGHQDFL 459
                 GFI  E   AL + + A ++R++ +R +E YN +  RI KT+Y   NF ++DFL
Sbjct: 387 ------GFIHSEVNRALNYPFYAIMDRVAKRRNIEHYNFDNTRILKTSYCSPNFANKDFL 446

Query: 460 EFAVKDFNTLQCIHRKELKELERW------------------------------------ 519
             +V+DFN  Q +HR+ELK   RW                                    
Sbjct: 447 FLSVEDFNNCQAMHREELKGKMRWVTENRLDELKFARSKSAYCYFSAAATFFAPDLSDAR 506

Query: 520 -----------------DANGGE-------------------DYCSKEVEIIFLALHSTI 579
                            D  G E                   +  S+ V+IIF +L  TI
Sbjct: 507 MSWAKNGVLTTVVDDFFDVGGSEEELKQLIRLVEIWDLDAITECSSQNVQIIFSSLKRTI 566

Query: 580 CEIGKRALAWQGRSVTRNVIDIWLALLESMRKEAEWLRNRVAPSMDEYMENGYVSFALGP 639
            EIG +    QGRSVT ++I+IWL LL SM KEAEW R+  AP MD+Y+ N YVSFALGP
Sbjct: 567 SEIGDKGFKLQGRSVTNHIIEIWLDLLYSMMKEAEWARDNHAPPMDDYISNAYVSFALGP 626

Query: 640 IVLPALYFVGPKLSEEVIGNCEYQKLFKLMSTCGRLLNDTRTFDVSTETSLHDLIVFTLR 699
           IVLP LY VGPKLSEE++ + E   LF+LMSTCGRLLND +T                  
Sbjct: 627 IVLPCLYLVGPKLSEEMVRHSECHTLFRLMSTCGRLLNDIQT------------------ 686

Query: 700 VLSFLYKPLLALLQRESNEGKLNAVSLCMINAGGNFTKEEAAEVIKGDVERTRRELLRLV 741
                        +RE  +GKLNA+ L MIN+GG  +KE A + +K  ++R R+ELLRLV
Sbjct: 687 ------------CERELKDGKLNAIPLYMINSGGETSKEAATQEMKSLIDRQRQELLRLV 746

BLAST of Spg004122 vs. ExPASy TrEMBL
Match: A0A1S4DWJ8 (ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489622 PE=4 SV=1)

HSP 1 Score: 1220.3 bits (3156), Expect = 0.0e+00
Identity = 620/798 (77.69%), Postives = 659/798 (82.58%), Query Frame = 0

Query: 20  SASLFPGLDVETKTKTGALRFEDTKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 79
           SASLFPGLDV+TKTKTGALRFEDTKERI+KLF  VELSVSAYDTAWVAMVPSPNSLNKPL
Sbjct: 17  SASLFPGLDVDTKTKTGALRFEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPNSLNKPL 76

Query: 80  FPECINWVLDNQHSDGSWGILHNHQLLMKATLLSTLACVLTLKRWNIGHDHMSKALNFIK 139
           FPECINWVLD+Q+ DGSWGILH+HQL+MKATLLSTLACVLTLKRWNIGHDHMSKAL+FIK
Sbjct: 77  FPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSKALSFIK 136

Query: 140 SNIASATDEKQHSPVGFDIIFPGMIEYAKDLNLNLPMTPMNVDALVQKKELELRRSNCEG 199
           SNIASATDE Q SPVGFDIIFPGMIEYAKDLNLNLP+  MNVDALVQKKELEL RSN EG
Sbjct: 137 SNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDALVQKKELEL-RSNSEG 196

Query: 200 GKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSLLQK 259
           GKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLF+SPSTTAVAFMHRNDDGCFNYLRS+LQK
Sbjct: 197 GKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFNYLRSILQK 256

Query: 260 FDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQGEENIFLDAST 319
           F SSVP IYPLDIYARLHMVDSLQKLGIDRHFK+EIRSVLDETYRCWMQGEENIFLDAST
Sbjct: 257 FHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKDEIRSVLDETYRCWMQGEENIFLDAST 316

Query: 320 CAMAFRMLRVEGYDVSSDQLTQFSEDLFSNCLGGYLKDFGASLELFKASQIIIYPEESVL 379
           CAMAFRMLRVEGYDVSSDQLTQFSEDLF NCLGG+LKDFGASLELFKASQIIIYP+E +L
Sbjct: 317 CAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGHLKDFGASLELFKASQIIIYPDEFIL 376

Query: 380 ENINSWTSRFLNHGLSSGSVCSDRTDGFIKQEAVSALEFTYKASLERLSNKRAMESYNGN 439
           ENINSWTSRFLNHGLS GSV SDRT+  +KQEAV+A EF Y ++LERLSNKRA+ESY+G+
Sbjct: 377 ENINSWTSRFLNHGLSGGSVHSDRTERLVKQEAVNAFEFPYNSTLERLSNKRALESYSGD 436

Query: 440 IVRISKTTYACLNFGHQDFLEFAVKDFNTLQCIHRKELKEL------------------- 499
           IVRISKTTYACLNFGHQDFLE AV+DFNTLQCIHRKELKEL                   
Sbjct: 437 IVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVIENKLDKLKFARQKL 496

Query: 500 -----------------------------------------------------ERWDANG 559
                                                                E+WDA+G
Sbjct: 497 AYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDASG 556

Query: 560 GEDYCSKEVEIIFLALHSTICEIGKRALAWQGRSVTRNVIDIWLALLESMRKEAEWLRNR 619
              YCSKEVEIIFLALHSTICEIG++AL WQGRSVTRNVIDIWLALLESMRKEAEWL+N+
Sbjct: 557 EAGYCSKEVEIIFLALHSTICEIGEKALPWQGRSVTRNVIDIWLALLESMRKEAEWLKNK 616

Query: 620 VAPSMDEYMENGYVSFALGPIVLPALYFVGPKLSEEVIGNCEYQKLFKLMSTCGRLLNDT 679
           V PS+DEYMENGYVSFALGPIVLP LYFVGPKL EE+IGNCEYQKLFKLMST GRLLNDT
Sbjct: 617 VVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIIGNCEYQKLFKLMSTSGRLLNDT 676

Query: 680 RTFDVSTETSLHDLIVFTLRVLSFLYKPLLALLQRESNEGKLNAVSLCMINAGGNFTKEE 739
           RTFD                              RES+EGKLNA+SL +I+AGG  +KEE
Sbjct: 677 RTFD------------------------------RESSEGKLNALSLYLISAGGKLSKEE 736

Query: 740 AAEVIKGDVERTRRELLRLVLQEKNSTIPRACMDLFWKMSCVVHLFYRKDDGFTSHELMN 746
           A E +KGDV+RTRRELLRLVLQE NSTIPRAC DLFWKMSCVVHLFYRKDDGFTSHELMN
Sbjct: 737 ATEAMKGDVDRTRRELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMN 782

BLAST of Spg004122 vs. ExPASy TrEMBL
Match: A0A1S3BG06 (ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489622 PE=4 SV=1)

HSP 1 Score: 1219.5 bits (3154), Expect = 0.0e+00
Identity = 620/800 (77.50%), Postives = 659/800 (82.38%), Query Frame = 0

Query: 20  SASLFPGLDVETKTKTGALRFEDTKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 79
           SASLFPGLDV+TKTKTGALRFEDTKERI+KLF  VELSVSAYDTAWVAMVPSPNSLNKPL
Sbjct: 17  SASLFPGLDVDTKTKTGALRFEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPNSLNKPL 76

Query: 80  FPECINWVLDNQHSDGSWGILHNHQLLMKATLLSTLACVLTLKRWNIGHDHMSKALNFIK 139
           FPECINWVLD+Q+ DGSWGILH+HQL+MKATLLSTLACVLTLKRWNIGHDHMSKAL+FIK
Sbjct: 77  FPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSKALSFIK 136

Query: 140 SNIASATDEKQHSPVGFDIIFPGMIEYAKDLNLNLPMTPMNVDALVQKKELELRR--SNC 199
           SNIASATDE Q SPVGFDIIFPGMIEYAKDLNLNLP+  MNVDALVQKKELELR   SN 
Sbjct: 137 SNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDALVQKKELELRSCCSNS 196

Query: 200 EGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSLL 259
           EGGKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLF+SPSTTAVAFMHRNDDGCFNYLRS+L
Sbjct: 197 EGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFNYLRSIL 256

Query: 260 QKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQGEENIFLDA 319
           QKF SSVP IYPLDIYARLHMVDSLQKLGIDRHFK+EIRSVLDETYRCWMQGEENIFLDA
Sbjct: 257 QKFHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKDEIRSVLDETYRCWMQGEENIFLDA 316

Query: 320 STCAMAFRMLRVEGYDVSSDQLTQFSEDLFSNCLGGYLKDFGASLELFKASQIIIYPEES 379
           STCAMAFRMLRVEGYDVSSDQLTQFSEDLF NCLGG+LKDFGASLELFKASQIIIYP+E 
Sbjct: 317 STCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGHLKDFGASLELFKASQIIIYPDEF 376

Query: 380 VLENINSWTSRFLNHGLSSGSVCSDRTDGFIKQEAVSALEFTYKASLERLSNKRAMESYN 439
           +LENINSWTSRFLNHGLS GSV SDRT+  +KQEAV+A EF Y ++LERLSNKRA+ESY+
Sbjct: 377 ILENINSWTSRFLNHGLSGGSVHSDRTERLVKQEAVNAFEFPYNSTLERLSNKRALESYS 436

Query: 440 GNIVRISKTTYACLNFGHQDFLEFAVKDFNTLQCIHRKELKEL----------------- 499
           G+IVRISKTTYACLNFGHQDFLE AV+DFNTLQCIHRKELKEL                 
Sbjct: 437 GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVIENKLDKLKFARQ 496

Query: 500 -------------------------------------------------------ERWDA 559
                                                                  E+WDA
Sbjct: 497 KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDA 556

Query: 560 NGGEDYCSKEVEIIFLALHSTICEIGKRALAWQGRSVTRNVIDIWLALLESMRKEAEWLR 619
           +G   YCSKEVEIIFLALHSTICEIG++AL WQGRSVTRNVIDIWLALLESMRKEAEWL+
Sbjct: 557 SGEAGYCSKEVEIIFLALHSTICEIGEKALPWQGRSVTRNVIDIWLALLESMRKEAEWLK 616

Query: 620 NRVAPSMDEYMENGYVSFALGPIVLPALYFVGPKLSEEVIGNCEYQKLFKLMSTCGRLLN 679
           N+V PS+DEYMENGYVSFALGPIVLP LYFVGPKL EE+IGNCEYQKLFKLMST GRLLN
Sbjct: 617 NKVVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIIGNCEYQKLFKLMSTSGRLLN 676

Query: 680 DTRTFDVSTETSLHDLIVFTLRVLSFLYKPLLALLQRESNEGKLNAVSLCMINAGGNFTK 739
           DTRTFD                              RES+EGKLNA+SL +I+AGG  +K
Sbjct: 677 DTRTFD------------------------------RESSEGKLNALSLYLISAGGKLSK 736

Query: 740 EEAAEVIKGDVERTRRELLRLVLQEKNSTIPRACMDLFWKMSCVVHLFYRKDDGFTSHEL 746
           EEA E +KGDV+RTRRELLRLVLQE NSTIPRAC DLFWKMSCVVHLFYRKDDGFTSHEL
Sbjct: 737 EEATEAMKGDVDRTRRELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHEL 785

BLAST of Spg004122 vs. ExPASy TrEMBL
Match: A0A5A7V5B3 (Ent-kaur-16-ene synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold357G00260 PE=4 SV=1)

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 616/799 (77.10%), Postives = 657/799 (82.23%), Query Frame = 0

Query: 21  ASLFPGLDVETKTKTGALRFEDTKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPLF 80
           +SLFPGLDV+TKTKTGALRFEDTKERI+KLF  VELSVSAYDTAWVAMVPSPNSLN+PLF
Sbjct: 92  SSLFPGLDVDTKTKTGALRFEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPNSLNRPLF 151

Query: 81  PECINWVLDNQHSDGSWGILHNHQLLMKATLLSTLACVLTLKRWNIGHDHMSKALNFIKS 140
           PECINWVLD+Q+ DGSWGILH+HQL+MKATLLSTLACVLTLKRWNIGHDHMSKAL+FIKS
Sbjct: 152 PECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSKALSFIKS 211

Query: 141 NIASATDEKQHSPVGFDIIFPGMIEYAKDLNLNLPMTPMNVDALVQKKELELRR--SNCE 200
           NIASATDE Q SPVGFDIIFPGMIEYAKDLNLNLP+  MNVDALVQKKELELR   SN E
Sbjct: 212 NIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDALVQKKELELRSCCSNSE 271

Query: 201 GGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSLLQ 260
           GGKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLF+SPSTTAVAFMHRNDDGCFNYLRS+LQ
Sbjct: 272 GGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFNYLRSILQ 331

Query: 261 KFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQGEENIFLDAS 320
           KF SSVP IYPLDIYARLHMVDSLQKLGIDRHFK+EIRSVLDETYRCWMQGEENIFLDAS
Sbjct: 332 KFHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKDEIRSVLDETYRCWMQGEENIFLDAS 391

Query: 321 TCAMAFRMLRVEGYDVSSDQLTQFSEDLFSNCLGGYLKDFGASLELFKASQIIIYPEESV 380
           TCAMAFRMLRVEGYDVSSDQLTQFSEDLF NCLGG+LKDFGASLELFKASQIIIYP+E +
Sbjct: 392 TCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGHLKDFGASLELFKASQIIIYPDEFI 451

Query: 381 LENINSWTSRFLNHGLSSGSVCSDRTDGFIKQEAVSALEFTYKASLERLSNKRAMESYNG 440
           LENINSWTSRFLNHGLS GSV  DRT+  +KQEAV+A EF Y ++LERLSNKRA+ESY+G
Sbjct: 452 LENINSWTSRFLNHGLSGGSVHFDRTERLVKQEAVNAFEFPYNSTLERLSNKRALESYSG 511

Query: 441 NIVRISKTTYACLNFGHQDFLEFAVKDFNTLQCIHRKELKEL------------------ 500
           +IVRISKTTYACLNFGHQDFLE AV+DFNTLQCIHRKELKEL                  
Sbjct: 512 DIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVIENKLDKLKFARQK 571

Query: 501 ------------------------------------------------------ERWDAN 560
                                                                 E+WDA+
Sbjct: 572 LAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDAS 631

Query: 561 GGEDYCSKEVEIIFLALHSTICEIGKRALAWQGRSVTRNVIDIWLALLESMRKEAEWLRN 620
           G   YCSKEVEIIFLALHSTICEIG++AL WQGRSVTRNVIDIWLALLESMRKEAEWL+N
Sbjct: 632 GEAGYCSKEVEIIFLALHSTICEIGEKALPWQGRSVTRNVIDIWLALLESMRKEAEWLKN 691

Query: 621 RVAPSMDEYMENGYVSFALGPIVLPALYFVGPKLSEEVIGNCEYQKLFKLMSTCGRLLND 680
           +V PS+DEYMENGYVSFALGPIVLP LYFVGPKL EE+IGNCEYQKLFKLMST GRLLND
Sbjct: 692 KVVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIIGNCEYQKLFKLMSTSGRLLND 751

Query: 681 TRTFDVSTETSLHDLIVFTLRVLSFLYKPLLALLQRESNEGKLNAVSLCMINAGGNFTKE 740
           TRTFD                              RES+EGKLNA+SL +I+AGG  +KE
Sbjct: 752 TRTFD------------------------------RESSEGKLNALSLYLISAGGKLSKE 811

Query: 741 EAAEVIKGDVERTRRELLRLVLQEKNSTIPRACMDLFWKMSCVVHLFYRKDDGFTSHELM 746
           EA E +KGDV+RTRRELLRLVLQE NSTIPRAC DLFWKMSCVVHLFYRKDDGFTSHELM
Sbjct: 812 EATEAMKGDVDRTRRELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELM 859

BLAST of Spg004122 vs. ExPASy TrEMBL
Match: A0A6J1D2F0 (ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016726 PE=4 SV=1)

HSP 1 Score: 1211.8 bits (3134), Expect = 0.0e+00
Identity = 608/798 (76.19%), Postives = 655/798 (82.08%), Query Frame = 0

Query: 20  SASLFPGLDVETKTKTGALRFEDTKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 79
           SAS FPGLDVETKTKTGAL FE+TKERIKK+F+KVELSVSAYDTAWVAMVPSPNSLN PL
Sbjct: 66  SASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNSLNDPL 125

Query: 80  FPECINWVLDNQHSDGSWGILHNHQLLMKATLLSTLACVLTLKRWNIGHDHMSKALNFIK 139
           FPECINWVLDNQH DGSWGI+HNH LLMKA+LLSTLAC LTLKRWNIGH+HMSKALNFIK
Sbjct: 126 FPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKALNFIK 185

Query: 140 SNIASATDEKQHSPVGFDIIFPGMIEYAKDLNLNLPMTPMNVDALVQKKELELRRSNCEG 199
           SNI SATDEKQ+SPVGFDIIFPGMIEYAKDL+LNLP+ P NVDAL+QKKEL+LRRSN EG
Sbjct: 186 SNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRRSNSEG 245

Query: 200 GKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSLLQK 259
           GKAYLAYVSEGIG+L+DWEMVMQYQRKNGSLFNSPSTTAVAFMH NDDGCFNYL SLLQK
Sbjct: 246 GKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQK 305

Query: 260 FDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQGEENIFLDAST 319
           FD+SVPTIYPLDIYARL MVD+LQ+LGIDRHFKEEIRSVLDETYRCWMQGEE+IFLDAST
Sbjct: 306 FDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEEDIFLDAST 365

Query: 320 CAMAFRMLRVEGYDVSSDQLTQFSEDLFSNCLGGYLKDFGASLELFKASQIIIYPEESVL 379
           CAMAFRMLR+ GYDVSSDQLTQFSEDLFSNCLGGYLKDFG+SLELFKASQIIIYP+ES+L
Sbjct: 366 CAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIIIYPDESIL 425

Query: 380 ENINSWTSRFLNHGLSSGSVCSDRTDGFIKQEAVSALEFTYKASLERLSNKRAMESYNGN 439
           ENINSWT+ FL+HGLSSGSV SDR D  +KQEA++ALEFTY  +LERL NKRAME+YN +
Sbjct: 426 ENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRAMENYNID 485

Query: 440 IVRISKTTYACLNFGHQDFLEFAVKDFNTLQCIHRKELKELER----------------- 499
           IVRISKTTY CLNFGHQDFLE AV+DFN LQ IH KELKELER                 
Sbjct: 486 IVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKLKFARQKL 545

Query: 500 -------------------------------------------------------WDANG 559
                                                                  WD NG
Sbjct: 546 AYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLVEKWDVNG 605

Query: 560 GEDYCSKEVEIIFLALHSTICEIGKRALAWQGRSVTRNVIDIWLALLESMRKEAEWLRNR 619
             DYCSKEVEIIFLALH T+CEI KRAL WQGRSV RNVIDIWLALL+SMRKEAEWL+N+
Sbjct: 606 EADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKEAEWLKNK 665

Query: 620 VAPSMDEYMENGYVSFALGPIVLPALYFVGPKLSEEVIGNCEYQKLFKLMSTCGRLLNDT 679
           +AP MDEYMENGY+SFALGPIVLPAL+FVGPKL EEVIG+CEY KLFKLMSTCGRLLNDT
Sbjct: 666 IAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDT 725

Query: 680 RTFDVSTETSLHDLIVFTLRVLSFLYKPLLALLQRESNEGKLNAVSLCMINAGGNFTKEE 739
           ++F                              +RES+EGKLNA+SLCMINAGG+FTKEE
Sbjct: 726 QSF------------------------------ERESSEGKLNAMSLCMINAGGDFTKEE 785

Query: 740 AAEVIKGDVERTRRELLRLVLQEKNSTIPRACMDLFWKMSCVVHLFYRKDDGFTSHELMN 746
           A+E IKG+VERTRRELLRLVLQEKNST+PRAC DLFWKMSCVVHLFYRKDDGFTSHELMN
Sbjct: 786 ASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMN 833

BLAST of Spg004122 vs. ExPASy TrEMBL
Match: A0A6J1D2L1 (ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016726 PE=4 SV=1)

HSP 1 Score: 1206.8 bits (3121), Expect = 0.0e+00
Identity = 608/800 (76.00%), Postives = 655/800 (81.88%), Query Frame = 0

Query: 20  SASLFPGLDVETKTKTGALRFEDTKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 79
           SAS FPGLDVETKTKTGAL FE+TKERIKK+F+KVELSVSAYDTAWVAMVPSPNSLN PL
Sbjct: 66  SASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNSLNDPL 125

Query: 80  FPECINWVLDNQHSDGSWGILHNHQLLMKATLLSTLACVLTLKRWNIGHDHMSKALNFIK 139
           FPECINWVLDNQH DGSWGI+HNH LLMKA+LLSTLAC LTLKRWNIGH+HMSKALNFIK
Sbjct: 126 FPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKALNFIK 185

Query: 140 SNIASATDEKQHSPVGFDIIFPGMIEYAKDLNLNLPMTPMNVDALVQKKELELR--RSNC 199
           SNI SATDEKQ+SPVGFDIIFPGMIEYAKDL+LNLP+ P NVDAL+QKKEL+LR  RSN 
Sbjct: 186 SNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRSCRSNS 245

Query: 200 EGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSLL 259
           EGGKAYLAYVSEGIG+L+DWEMVMQYQRKNGSLFNSPSTTAVAFMH NDDGCFNYL SLL
Sbjct: 246 EGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNYLHSLL 305

Query: 260 QKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQGEENIFLDA 319
           QKFD+SVPTIYPLDIYARL MVD+LQ+LGIDRHFKEEIRSVLDETYRCWMQGEE+IFLDA
Sbjct: 306 QKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEEDIFLDA 365

Query: 320 STCAMAFRMLRVEGYDVSSDQLTQFSEDLFSNCLGGYLKDFGASLELFKASQIIIYPEES 379
           STCAMAFRMLR+ GYDVSSDQLTQFSEDLFSNCLGGYLKDFG+SLELFKASQIIIYP+ES
Sbjct: 366 STCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIIIYPDES 425

Query: 380 VLENINSWTSRFLNHGLSSGSVCSDRTDGFIKQEAVSALEFTYKASLERLSNKRAMESYN 439
           +LENINSWT+ FL+HGLSSGSV SDR D  +KQEA++ALEFTY  +LERL NKRAME+YN
Sbjct: 426 ILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRAMENYN 485

Query: 440 GNIVRISKTTYACLNFGHQDFLEFAVKDFNTLQCIHRKELKELER--------------- 499
            +IVRISKTTY CLNFGHQDFLE AV+DFN LQ IH KELKELER               
Sbjct: 486 IDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKLKFARQ 545

Query: 500 ---------------------------------------------------------WDA 559
                                                                    WD 
Sbjct: 546 KLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLVEKWDV 605

Query: 560 NGGEDYCSKEVEIIFLALHSTICEIGKRALAWQGRSVTRNVIDIWLALLESMRKEAEWLR 619
           NG  DYCSKEVEIIFLALH T+CEI KRAL WQGRSV RNVIDIWLALL+SMRKEAEWL+
Sbjct: 606 NGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKEAEWLK 665

Query: 620 NRVAPSMDEYMENGYVSFALGPIVLPALYFVGPKLSEEVIGNCEYQKLFKLMSTCGRLLN 679
           N++AP MDEYMENGY+SFALGPIVLPAL+FVGPKL EEVIG+CEY KLFKLMSTCGRLLN
Sbjct: 666 NKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTCGRLLN 725

Query: 680 DTRTFDVSTETSLHDLIVFTLRVLSFLYKPLLALLQRESNEGKLNAVSLCMINAGGNFTK 739
           DT++F                              +RES+EGKLNA+SLCMINAGG+FTK
Sbjct: 726 DTQSF------------------------------ERESSEGKLNAMSLCMINAGGDFTK 785

Query: 740 EEAAEVIKGDVERTRRELLRLVLQEKNSTIPRACMDLFWKMSCVVHLFYRKDDGFTSHEL 746
           EEA+E IKG+VERTRRELLRLVLQEKNST+PRAC DLFWKMSCVVHLFYRKDDGFTSHEL
Sbjct: 786 EEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHEL 835

BLAST of Spg004122 vs. TAIR 10
Match: AT1G79460.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 677.6 bits (1747), Expect = 1.2e-194
Identity = 370/802 (46.13%), Postives = 497/802 (61.97%), Query Frame = 0

Query: 20  SASLFPGLDVETKTKTGALRFEDTKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 79
           SA+L   LD E +T+   + FE TKE+I+K+ +KVELSVSAYDT+WVAMVPSP+S N PL
Sbjct: 15  SATLERRLDSEVQTRANNVSFEQTKEKIRKMLEKVELSVSAYDTSWVAMVPSPSSQNAPL 74

Query: 80  FPECINWVLDNQHSDGSWGI-LHNHQLLMKATLLSTLACVLTLKRWNIGHDHMSKALNFI 139
           FP+C+ W+LDNQH DGSWG+  H+HQ L K  L STLA +L LK+W IG   ++K L FI
Sbjct: 75  FPQCVKWLLDNQHEDGSWGLDNHDHQSLKKDVLSSTLASILALKKWGIGERQINKGLQFI 134

Query: 140 KSNIASATDEKQHSPVGFDIIFPGMIEYAKDLNLNLPMTPMNVDALVQKKELELRRSN-- 199
           + N A  TDE    P GFDIIFPGMI+YA+DLNL +P+    VD +++K++L+L+  +  
Sbjct: 135 ELNSALVTDETIQKPTGFDIIFPGMIKYARDLNLTIPLGSEVVDDMIRKRDLDLKCDSEK 194

Query: 200 -CEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRS 259
             +G +AYLAYV EG   L+DW+++++YQRKNGSLF+SP+TTA AF    +DGC  YL S
Sbjct: 195 FSKGREAYLAYVLEGTRNLKDWDLIVKYQRKNGSLFDSPATTAAAFTQFGNDGCLRYLCS 254

Query: 260 LLQKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQGEENIFL 319
           LLQKF+++VP++YP D YARL ++ +L+ LGIDR FK EI+S+LDETYR W++G+E I L
Sbjct: 255 LLQKFEAAVPSVYPFDQYARLSIIVTLESLGIDRDFKTEIKSILDETYRYWLRGDEEICL 314

Query: 320 DASTCAMAFRMLRVEGYDVSSDQLTQFSEDL-FSNCLGGYLKDFGASLELFKASQIIIYP 379
           D +TCA+AFR+L   GYDVS D L  F+E+  FS+ L GY+K+  + LELFKA+Q   YP
Sbjct: 315 DLATCALAFRLLLAHGYDVSYDPLKPFAEESGFSDTLEGYVKNTFSVLELFKAAQ--SYP 374

Query: 380 EESVLENINSWTSRFLNHGLSSGSVCSDRTDGFIKQEAVSALEFTYKASLERLSNKRAME 439
            ES L+    WT ++L   LSS    S R D ++K+E   AL F   ASLER  ++R + 
Sbjct: 375 HESALKKQCCWTKQYLEMELSSWVKTSVR-DKYLKKEVEDALAFPSYASLERSDHRRKI- 434

Query: 440 SYNGNIV---RISKTTYACLNFGHQDFLEFAVKDFNTLQCIHRKELKEL----------- 499
             NG+ V   R++KT+Y   N    D L+ AV DFN  Q IHR+E++ L           
Sbjct: 435 -LNGSAVENTRVTKTSYRLHNICTSDILKLAVDDFNFCQSIHREEMERLDRWIVENRLQE 494

Query: 500 ------------------------------------------------------------ 559
                                                                       
Sbjct: 495 LKFARQKLAYCYFSGAATLFSPELSDARISWAKGGVLTTVVDDFFDVGGSKEELENLIHL 554

Query: 560 -ERWDANGGEDYCSKEVEIIFLALHSTICEIGKRALAWQGRSVTRNVIDIWLALLESMRK 619
            E+WD NG  +Y S+ VEIIF  L  TI E G +A  +QGR+VT +++ IWL LL+SM +
Sbjct: 555 VEKWDLNGVPEYSSEHVEIIFSVLRDTILETGDKAFTYQGRNVTHHIVKIWLDLLKSMLR 614

Query: 620 EAEWLRNRVAPSMDEYMENGYVSFALGPIVLPALYFVGPKLSEEVIGNCEYQKLFKLMST 679
           EAEW  ++  PS+++YMEN Y+SFALGPIVLPA Y +GP L E+ + + +Y +L+KL+ST
Sbjct: 615 EAEWSSDKSTPSLEDYMENAYISFALGPIVLPATYLIGPPLPEKTVDSHQYNQLYKLVST 674

Query: 680 CGRLLNDTRTFDVSTETSLHDLIVFTLRVLSFLYKPLLALLQRESNEGKLNAVSLCMINA 739
            GRLLND + F                              +RES EGKLNAVSL M + 
Sbjct: 675 MGRLLNDIQGF------------------------------KRESAEGKLNAVSLHMKHE 734

Query: 740 GGNFTKEEAAEVIKGDVERTRRELLRLVLQEKNSTIPRACMDLFWKMSCVVHLFYRKDDG 742
             N +KE   E +KG  ER R EL +LVL+EK S +PR C + F KMS V++LFYRKDDG
Sbjct: 735 RDNRSKEVIIESMKGLAERKREELHKLVLEEKGSVVPRECKEAFLKMSKVLNLFYRKDDG 781

BLAST of Spg004122 vs. TAIR 10
Match: AT4G02780.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 3.8e-68
Identity = 154/476 (32.35%), Postives = 248/476 (52.10%), Query Frame = 0

Query: 25  PGLDVETKTKTGALRFEDTKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPLFPECI 84
           P + V + +       +  K  ++ L D  E+++SAYDTAWVA++ + +    P FP  +
Sbjct: 83  PQISVGSNSNAFKEAVKSVKTILRNLTDG-EITISAYDTAWVALIDAGD--KTPAFPSAV 142

Query: 85  NWVLDNQHSDGSWGILHNHQLLMKATLLSTLACVLTLKRWNIGHDHMSKALNFIKSNIAS 144
            W+ +NQ SDGSWG    +       L++TLACV+ L+ WN+     +K + F + NI  
Sbjct: 143 KWIAENQLSDGSWG--DAYLFSYHDRLINTLACVVALRSWNLFPHQCNKGITFFRENIGK 202

Query: 145 ATDEK-QHSPVGFDIIFPGMIEYAKDLNLNLPMTPMNVDALVQKKELELRRSNCE---GG 204
             DE  +H P+GF++ FP ++E A+ +N+++P     +  +  KKEL+L R   E     
Sbjct: 203 LEDENDEHMPIGFEVAFPSLLEIARGINIDVPYDSPVLKDIYAKKELKLTRIPKEIMHKI 262

Query: 205 KAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSLLQKF 264
              L +  EG+  L DWE +++ Q ++GS   SPS+TA AFM   D  C  YLR+ +++F
Sbjct: 263 PTTLLHSLEGMRDL-DWEKLLKLQSQDGSFLFSPSSTAFAFMQTRDSNCLEYLRNAVKRF 322

Query: 265 DSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQ-----GEENIFL 324
           +  VP ++P+D++  + +VD LQ+LGI R+F+EEI+  LD  +R W          +   
Sbjct: 323 NGGVPNVFPVDLFEHIWIVDRLQRLGISRYFEEEIKECLDYVHRYWTDNGICWARCSHVQ 382

Query: 325 DASTCAMAFRMLRVEGYDVSSDQLTQFSEDLFSNCLGGYLKDFGASL-ELFKASQIIIYP 384
           D    AMAFR+LR  GY VS+D    F ++    C  G        +  L++ASQ + +P
Sbjct: 383 DIDDTAMAFRLLRQHGYQVSADVFKNFEKEGEFFCFVGQSNQAVTGMFNLYRASQ-LAFP 442

Query: 385 EESVLENINSWTSRFL------NHGLSSGSVCSDRTDGFIKQEAVSALEFTYKASLERLS 444
            E +L+N   ++  +L         +    +  D     +  E   ALE  + ASL R+ 
Sbjct: 443 REEILKNAKEFSYNYLLEKREREELIDKWIIMKD-----LPGEIGFALEIPWYASLPRVE 502

Query: 445 NKRAMESYNG-NIVRISKTTYACLNFGHQDFLEFAVKDFNTLQCIHRKELKELERW 484
            +  ++ Y G N V I KT Y      +  +LE A +D+N  Q  H+ E    ++W
Sbjct: 503 TRFYIDQYGGENDVWIGKTLYRMPYVNNNGYLELAKQDYNNCQAQHQLEWDIFQKW 546

BLAST of Spg004122 vs. TAIR 10
Match: AT1G61120.1 (terpene synthase 04 )

HSP 1 Score: 212.6 bits (540), Expect = 1.1e-54
Identity = 211/800 (26.38%), Postives = 345/800 (43.12%), Query Frame = 0

Query: 56  LSVSAYDTAWVAMVPSPNSLN----KPLFPECINWVLDNQHS-DGSWGILHNHQLLM--- 115
           +S SAYDTAW++M+    +++    KP+F  C++W++ NQ++ +G W    ++  +    
Sbjct: 36  VSPSAYDTAWLSMIEEDINVDDNELKPMFQGCLDWIMCNQNAREGFWMNSTSYTTVADGR 95

Query: 116 -------KATLLSTLACVLTLKRWNIGHDHMSKALNFIKSN----IASATDEKQHSPVGF 175
                     L STLACV+ L++WNIG  H+ K   +I+ N    I    +E+   P  F
Sbjct: 96  DEDGEKDMCILTSTLACVVALQKWNIGCFHLHKGTRYIERNTEMIIGKYINEEGSYPRWF 155

Query: 176 DIIFPGMIEYAKDLNLNLPMTPMNVDAL----VQKKELELRRS---NCEGGKAYLAYVSE 235
            I F G++E A+ L L+   +   ++ +     Q++E+  R     +C   K  LAY+  
Sbjct: 156 AIKFTGILELAQKLGLHFVFSSRCIEMIKGMFYQRQEIIQREKLVHDC-NYKPLLAYLEV 215

Query: 236 GIGKL----QDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSLLQKFDSSVP 295
              KL    Q+  +V      +GSLF SPS TA AFM   +  C  YL++L+QK  + VP
Sbjct: 216 LPSKLYVTNQEDIIVKSLDSMDGSLFQSPSATASAFMLTRNTKCLAYLQNLVQKCPNGVP 275

Query: 296 TIYPL-DIYARLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQGEENIF--LDASTCA- 355
             YPL +   +L MV+ ++  G+   F  EI  VL++ Y  +   EE  F  +  S  A 
Sbjct: 276 QKYPLNEDLIKLSMVNLIESTGLGEFFGIEIEHVLEQVYSRY---EEKDFERMPMSYLAD 335

Query: 356 ------MAFRMLRVEGYDVSSDQLTQFSEDLFS-NCLGGYLKDF-GASLELFKASQIIIY 415
                 +AFRMLR+ G DVS      F  D  + N L   +  F    L +++A+  +++
Sbjct: 336 QLHKDSLAFRMLRMHGRDVSPRSFCWFLNDQETRNHLERNIDSFLLVILSVYRATD-LMF 395

Query: 416 PEESVLENINSWTSRFLNHGLSSGSVCSDRTDGFIKQEAV-SALEFTYKASLERLSNKRA 475
           P E  L+    +T   L    S            IK++ +   L   + A L+ L ++  
Sbjct: 396 PGEHDLQEAREYTRNLLEKRRS------------IKEKMIMHELSTPWIARLKHLDHRMW 455

Query: 476 MESYNGNIVRISKTTYACLNFGHQDFL-EFAVKDFNTLQCIHRKELKELERW-------D 535
           +E  N N++ + K ++  L+  + D L   A ++F   Q  + +EL+EL  W       D
Sbjct: 456 IEDKNSNVLSMEKASFLRLHSSYSDKLTHLAARNFEFQQAKYCRELEELTMWVKKWGLSD 515

Query: 536 ANGGED---YC--------SKEVEIIF------LALHSTICE--------------IGKR 595
              G +   YC          E  I F       A+  TI +              + K 
Sbjct: 516 IGFGREKTTYCYFATVTSLPYEYAIKFGKLAAKTAILITIADDFFDEKGSFNDLEGLTKA 575

Query: 596 ALAWQGRSVTR--NVI----------------------------DIWLALLESMRKEAEW 655
            L W+G  +    N+I                            +IW    ES  +EAEW
Sbjct: 576 VLRWEGEELKSYGNIIFRALDDIVRETANTCRTHHKTDIIVHLRNIWGETFESWLREAEW 635

Query: 656 LRNRVAPSMDEYMENGYVSFALGPIVLPALYFVGPKLSEEVIGNCEYQKLFKLMSTCGRL 715
            +     SMDEY+ NG +S A   I L     + P      +    Y  +  L+    RL
Sbjct: 636 SKKGHTSSMDEYIRNGMISIAAHTIALSISCLMEPCFPHNKLKPGNYDSITTLLMIIPRL 695

Query: 716 LNDTRTFDVSTETSLHDLIVFTLRVLSFLYKPLLALLQRESNEGKLNAVSLCMINAGGNF 743
           LND +++                              Q+E  +GK+N+V L M N  G  
Sbjct: 696 LNDLQSY------------------------------QKEQEQGKMNSVLLHMKNHPGLE 755

BLAST of Spg004122 vs. TAIR 10
Match: AT4G16730.1 (terpene synthase 02 )

HSP 1 Score: 93.6 bits (231), Expect = 7.2e-19
Identity = 102/416 (24.52%), Postives = 166/416 (39.90%), Query Frame = 0

Query: 275 RLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQGE-ENIFLDASTCAMAFRMLRVEGYD 334
           +L M+DSLQ+LGI  H+K EI  +L + +     GE E    D    ++ F +LR  G+D
Sbjct: 44  QLEMIDSLQRLGISYHYKHEIHDILRKIHD--QHGEIERETQDLHATSLEFILLRQHGFD 103

Query: 335 VSSDQLTQFSEDLFSNCLGGYLK----DFGASLELFKASQIIIYPEESVLEN---INSWT 394
           VS D       D+F +  G + K    D    L L++AS   +  E  + E     N   
Sbjct: 104 VSQDAF-----DVFISETGEFRKTLHSDIKGLLSLYEASYFSMDSEFKLKETRIYANKRL 163

Query: 395 SRFLNHGLSSGSVCSDRTDGFIKQEAVSALEFTYKASLERLSNKRAMESYNGNIVRISKT 454
           S F+    SS ++C +  + +I +    ALE  Y  S+ RL  +  +  Y          
Sbjct: 164 SEFVAE--SSKTICRE-DETYILEMVKRALETPYHWSIRRLEARWYINVYEKK------- 223

Query: 455 TYACLNFGHQDFLEFAVKDFNTLQCIHRKELKELERWDANGG------------------ 514
                +  +   LEFA  DFN LQ  H++ELK +  W  + G                  
Sbjct: 224 -----HEMNPLLLEFAAIDFNMLQANHQEELKLISSWWNSTGLMKQLDFVRDRITESYFW 283

Query: 515 ---------EDYCSK-----------------------EVEII----------------- 574
                      YC K                       E+E+                  
Sbjct: 284 TIGIFYEPEFKYCRKILTKIFMLIVIMDDIYDIYGTLEELELFTNVVEKWDVNHVERLPN 343

Query: 575 -----FLALHSTICEIGKRALAWQGRSVTRNVIDIWLALLESMRKEAEWLRNRVAPSMDE 610
                FL L++ I +IG   L  +G +V   +  +W  L ++   E++W +    PS +E
Sbjct: 344 YMRMCFLFLYNEINQIGYDVLRDKGLNVIPYLKQVWTDLFKTFLTESKWYKTGHKPSFEE 403

BLAST of Spg004122 vs. TAIR 10
Match: AT4G16740.1 (terpene synthase 03 )

HSP 1 Score: 88.6 bits (218), Expect = 2.3e-17
Identity = 92/404 (22.77%), Postives = 178/404 (44.06%), Query Frame = 0

Query: 275 RLHMVDSLQKLGIDRHFKEEIRSVLDETYRCWMQG-----EENIFLDASTCAMAFRMLRV 334
           +L ++D+LQ+LG+  HF++EI+  L   +   ++      + N + D    A+ FR+LR 
Sbjct: 77  QLELIDTLQRLGVSYHFEQEIKKTLTNVHVKNVRAHKNRIDRNRWGDLYATALEFRLLRQ 136

Query: 335 EGYDVSSDQLTQFSEDLFSNCLGGYL--KDFGASLELFKASQIIIYPEESVLENINSWTS 394
            G+ +        ++D+F   +G  L  KD    L L++AS +    +  + E+I   T 
Sbjct: 137 HGFSI--------AQDVFDGNIGVDLDDKDIKGILSLYEASYLSTRIDTKLKESIYYTTK 196

Query: 395 RFLNHGLSSGSVCSDRTDGF-IKQEAVSALEFTYKASLERLSNKRAMESY------NGNI 454
           R          V  + T  + +++  + ALE  Y   + RL  +  +E Y      N  +
Sbjct: 197 RLRKF----VEVNKNETKSYTLRRMVIHALEMPYHRRVGRLEARWYIEVYGERHDMNPIL 256

Query: 455 VRISKTTYACLNFGHQDFLEFA---------------VKD------FNTLQCIHRKE--- 514
           + ++K  +  +   HQD L+                 V+D      F+++  ++  E   
Sbjct: 257 LELAKLDFNFVQAIHQDELKSLSSWWSKTGLTKHLDFVRDRITEGYFSSVGVMYEPEFAY 316

Query: 515 ------------------------LKEL-------ERWDANGGEDYCSKEVEIIFLALHS 574
                                   L+EL       E+WD N  E+     +++ FL L +
Sbjct: 317 HRQMLTKVFMLITTIDDIYDIYGTLEELQLFTTIVEKWDVNRLEE-LPNYMKLCFLCLVN 376

Query: 575 TICEIGKRALAWQGRSVTRNVIDIWLALLESMRKEAEWLRNRVAPSMDEYMENGYVSFAL 610
            I +IG   L  +G +V   + + W  +  +  KEA+W ++   P+ +EYM+NG++S ++
Sbjct: 377 EINQIGYFVLRDKGFNVIPYLKESWADMCTTFLKEAKWYKSGYKPNFEEYMQNGWISSSV 436