Spg003486 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg003486 vs. NCBI nr
Match: XP_004139387.1 (myosin-binding protein 1 [Cucumis sativus] >XP_011648739.1 myosin-binding protein 1 [Cucumis sativus] >KGN60783.1 hypothetical protein Csa_019501 [Cucumis sativus])

HSP 1 Score: 1273.8 bits (3295), Expect = 0.0e+00
Identity = 677/905 (74.81%), Postives = 737/905 (81.44%), Query Frame = 0

Query: 51  MGTSSVEAHTERDLFTSLLSAVFEWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRL 110
           MGTSSVEA +ER LFTSLLSAV EWLLICMLFVDSIFSFFITKCAHFWKLH PCLLCSRL
Sbjct: 1   MGTSSVEARSERSLFTSLLSAVSEWLLICMLFVDSIFSFFITKCAHFWKLHTPCLLCSRL 60

Query: 111 DHVFSSEKRGYLWKLICGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKSNSET 170
           DH+F SEKRGY+W LIC KH+ E+S LVLCHAHNKLVNVHEMCENCLFSFAT  KSNSET
Sbjct: 61  DHIFGSEKRGYVWNLICSKHKVELSSLVLCHAHNKLVNVHEMCENCLFSFATFKKSNSET 120

Query: 171 YRLLVGKLGEDPRHVIDRHPLLGDQKHDSLSEKHCSCCKEPYVSRAFVQKLIQTRSNGLE 230
           YRLLVGKLGEDP   IDR PLL DQK+D+ S+K CSCCKE YV R F Q LIQTRS+GLE
Sbjct: 121 YRLLVGKLGEDPYPGIDRDPLLADQKYDTSSQKCCSCCKELYVPRGFAQSLIQTRSSGLE 180

Query: 231 TKDLDVPLSSSVVHCKEDFQEASSNALPQVQYRELKITSDTESDGYGSTLGVETANS--- 290
            +DLDVPLSSS VHC+EDFQ++SSN LP VQYRELKITSDTES+G GS LGVE ANS   
Sbjct: 181 AEDLDVPLSSSAVHCEEDFQDSSSNPLPHVQYRELKITSDTESEGNGSILGVEAANSLKD 240

Query: 291 ------------------------------------------------------------ 350
                                                                       
Sbjct: 241 DLTIQDVNMEPNFISLASNLTSTKLMEPALAPEPFVLEPLLTPYVQNRELKINPDTESDG 300

Query: 351 ------------KDDFTVQGVHMEPNLISLDSNLTSTKLVEPALAPEPLVIEPLVSLGDA 410
                       KDD TVQGV  EPN+I+LDSNLTS KLVEPALAPEPLV+EPLV L D 
Sbjct: 301 NGSSLRVETTNFKDDLTVQGVTTEPNIIALDSNLTSAKLVEPALAPEPLVLEPLVFLDDT 360

Query: 411 LPPVECGVSIGHGLDELTPKHVEANGVFSSPTDLLSLDNMVSSSNTIATSIEALDESYVT 470
           LPPVECGV IGHGLDE+TPKHVE NGVFSSPTDLL +DN+VSSSNTI T +EA++ES VT
Sbjct: 361 LPPVECGVLIGHGLDEVTPKHVEVNGVFSSPTDLLLIDNVVSSSNTIETPVEAVEESCVT 420

Query: 471 RNEEYETECRETEKAEILPTKATSEAGSEAQPVSSDAVQMAPNILEMGDAYKLALGARGG 530
           R+EEYE E R TEKAEILPTKATSEAGSE QPVSSD+ QMAP +LE+GDAYKLA+GARGG
Sbjct: 421 RSEEYEKESRGTEKAEILPTKATSEAGSEVQPVSSDSAQMAPIMLELGDAYKLAVGARGG 480

Query: 531 RQLSGKLSEQWLERDSSRVSEDLKLLLSQLSFNRMNDQSRDMSPRLSINGDESRNFDVSS 590
           RQLSGKL EQW+ ++SS+VSEDLKLLLSQLSFNRMNDQSRDMSPRLS+NGDE RNFD SS
Sbjct: 481 RQLSGKLLEQWIGKESSKVSEDLKLLLSQLSFNRMNDQSRDMSPRLSVNGDEVRNFDYSS 540

Query: 591 AVGMQMLQKRISLERNESGLESLDGSIVTEIEGENVVDRLKRQIEYDKRIMNSLYKELDE 650
           AVGMQMLQ+RISLERNESGLESLDGSI++EI+GENV DRLKRQ+EYDK++M+SLYKEL+E
Sbjct: 541 AVGMQMLQRRISLERNESGLESLDGSIISEIDGENVADRLKRQVEYDKKVMSSLYKELEE 600

Query: 651 ERNASAIATNQAMAMITRLQEEKATLHMEALQCLRMMEEQNEYDDDALQKANDLISEKDK 710
           ERNASAIATNQAMAMITRLQEEKA LHMEALQCLRMMEEQ+EYDDDALQKANDLI+EKDK
Sbjct: 601 ERNASAIATNQAMAMITRLQEEKANLHMEALQCLRMMEEQSEYDDDALQKANDLITEKDK 660

Query: 711 EIQDLEAELEFYRINFPNAYTIDNLVETSNVKERDIRVVHLESNQIGTIGYGNSVAGEPD 770
           EIQDLEAELEFYRINFPNAYTIDNLVETS VKERDI VVHLESNQ GTIG GN +AG+PD
Sbjct: 661 EIQDLEAELEFYRINFPNAYTIDNLVETS-VKERDIGVVHLESNQFGTIGNGNLIAGKPD 720

Query: 771 IHEKVGSEGSTYNNLLLEFEDEKLDIMQCLKMLENRLHLFSNNGDKIDLSNGEYFG---- 830
           +HEKVGSEGSTYNNLLLEFEDEKL+IMQ LK LEN LHLFSN+G K+DLSNGEY G    
Sbjct: 721 LHEKVGSEGSTYNNLLLEFEDEKLNIMQRLKKLENMLHLFSNDGIKMDLSNGEYIGNERS 780

Query: 831 ------DFDSDNRKLEDGKDHACLPGKDLHDDDDRLPSLANPSLSKESSELDCSDRNSLL 871
                 D D D+RKLED + HACLPG+D H +DD LPSL NPS  KES+ELDCSDRNSLL
Sbjct: 781 FSSGTNDLDLDDRKLEDREHHACLPGEDAHIEDDHLPSLTNPSFDKESNELDCSDRNSLL 840

BLAST of Spg003486 vs. NCBI nr
Match: XP_008456276.1 (PREDICTED: myosin-binding protein 1-like [Cucumis melo] >XP_008456282.1 PREDICTED: myosin-binding protein 1-like [Cucumis melo] >XP_016902090.1 PREDICTED: myosin-binding protein 1-like [Cucumis melo])

HSP 1 Score: 1231.5 bits (3185), Expect = 0.0e+00
Identity = 659/906 (72.74%), Postives = 724/906 (79.91%), Query Frame = 0

Query: 51  MGTSSVEAHTERDLFTSLLSAVFEWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRL 110
           MGTSSVEA TER LFT+L+SAVFEWLLICMLF DSIFSFFITKCAHFWKL  PCLLCSRL
Sbjct: 1   MGTSSVEARTERSLFTALVSAVFEWLLICMLFADSIFSFFITKCAHFWKLCTPCLLCSRL 60

Query: 111 DHVFSSEKRGYLWKLICGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKSNSET 170
           DH+F SEKRGY+W LIC KH+ E+S LVLCHAHNKLVNVHEMCENCLFSFAT NKSNSET
Sbjct: 61  DHIFGSEKRGYIWNLICSKHKLELSSLVLCHAHNKLVNVHEMCENCLFSFATFNKSNSET 120

Query: 171 YRLLVGKLGEDPRHVIDRHPLLGDQKHDSLSEKHCSCCKEPYVSRAFVQKLIQTRSNGLE 230
           YRLLVGKLG+DP   IDR PLLGDQK+D+LS+K CSCCKE YV R F Q LIQTRS+GLE
Sbjct: 121 YRLLVGKLGKDPYPRIDRDPLLGDQKYDTLSQKCCSCCKELYVPRGFAQSLIQTRSSGLE 180

Query: 231 TKDLDVPLSSSVVHCKEDFQEASSNALPQVQYRELKITS--------------------- 290
            +DLDVPL+SS VH   D QE S+N  P VQYRELKITS                     
Sbjct: 181 AEDLDVPLASSAVHYDGDSQECSNNPRPHVQYRELKITSDTESEGNGSILGVERANSLKD 240

Query: 291 -------------------------------------------------------DTESD 350
                                                                  DTESD
Sbjct: 241 DLTIQDVNMEPNFISLASNLTSTKLIEPALAPEPLVLEPLATTPYVQNRELKITPDTESD 300

Query: 351 GYGSTLGVETANSKDDFTVQGVHMEPNLISLDSNLTSTKLVEPALAPEPLVIEPLVSLGD 410
           G GSTL VET NSKDD TV GV+ EPN+ISLDSNLTS KLVEPALAPEPLV+EPLVSL D
Sbjct: 301 GNGSTLRVETTNSKDDLTVLGVNTEPNIISLDSNLTSAKLVEPALAPEPLVLEPLVSLDD 360

Query: 411 ALPPVECGVSIGHGLDELTPKHVEANGVFSSPTDLLSLDNMVSSSNTIATSIEALDESYV 470
           ALPP ECGV IGHGLDE+TPK VE NG FSSP+DLL +DN+VSSSNT  T +EA++ES V
Sbjct: 361 ALPPAECGVLIGHGLDEVTPKLVEVNGFFSSPSDLLPIDNVVSSSNTKETPVEAVEESCV 420

Query: 471 TRNEEYETECRETEKAEILPTKATSEAGSEAQPVSSDAVQMAPNILEMGDAYKLALGARG 530
            R+EEYE E R TEKAEILPTKATSEAGSE QPVSSD+ QM PN+LE+GDAYKLA+GARG
Sbjct: 421 ERSEEYEKESRGTEKAEILPTKATSEAGSEVQPVSSDSAQMVPNMLELGDAYKLAVGARG 480

Query: 531 GRQLSGKLSEQWLERDSSRVSEDLKLLLSQLSFNRMNDQSRDMSPRLSINGDESRNFDVS 590
           GRQLSGKL EQW+ ++SS+VSEDLKLLL+QLSFNR+NDQSR+MSPRLS+NGDE RNFD  
Sbjct: 481 GRQLSGKLLEQWIGKESSKVSEDLKLLLTQLSFNRLNDQSREMSPRLSVNGDEVRNFDYL 540

Query: 591 SAVGMQMLQKRISLERNESGLESLDGSIVTEIEGENVVDRLKRQIEYDKRIMNSLYKELD 650
           SAVGMQMLQKRISLERNESG+ESLDGSI++EI+GEN+ DRLKRQIEYDK++M+SLYKEL+
Sbjct: 541 SAVGMQMLQKRISLERNESGVESLDGSIISEIDGENMADRLKRQIEYDKKVMSSLYKELE 600

Query: 651 EERNASAIATNQAMAMITRLQEEKATLHMEALQCLRMMEEQNEYDDDALQKANDLISEKD 710
           EERNASAIA NQAMAMITRLQEEKA LHMEAL CLRMMEEQ+EYDDDALQKANDLI+EKD
Sbjct: 601 EERNASAIAANQAMAMITRLQEEKANLHMEALHCLRMMEEQSEYDDDALQKANDLITEKD 660

Query: 711 KEIQDLEAELEFYRINFPNAYTIDNLVETSNVKERDIRVVHLESNQIGTIGYGNSVAGEP 770
           KEIQDLEAELEFYRINFPNAYTIDNLVETS VKERDIRVVHLESNQI  IG  N V G+P
Sbjct: 661 KEIQDLEAELEFYRINFPNAYTIDNLVETS-VKERDIRVVHLESNQIEPIGNRNLVTGKP 720

Query: 771 DIHEKVGSEGSTYNNLLLEFEDEKLDIMQCLKMLENRLHLFSNNGDKIDLSNGEYFG--- 830
           D+HE+VGSEGSTYNNLLLEFEDEKL+I Q LK LEN LHLFSN+G K+DLSNG+YFG   
Sbjct: 721 DLHEEVGSEGSTYNNLLLEFEDEKLNIKQHLKKLENMLHLFSNDGVKMDLSNGKYFGNKR 780

Query: 831 -------DFDSDNRKLEDGKDHACLPGKDLHDDDDRLPSLANPSLSKESSELDCSDRNSL 871
                  D D D+RKLED + HACLPG+D H +DD LPSL N S  KESSELDCSD+NS 
Sbjct: 781 SFSSGTNDLDLDDRKLEDREHHACLPGEDAHIEDDHLPSLTNSSFDKESSELDCSDKNSP 840

BLAST of Spg003486 vs. NCBI nr
Match: XP_038877642.1 (myosin-binding protein 1-like [Benincasa hispida] >XP_038877643.1 myosin-binding protein 1-like [Benincasa hispida] >XP_038877644.1 myosin-binding protein 1-like [Benincasa hispida])

HSP 1 Score: 1228.0 bits (3176), Expect = 0.0e+00
Identity = 659/900 (73.22%), Postives = 724/900 (80.44%), Query Frame = 0

Query: 51  MGTSSVEAHTERDLFTSLLSAVFEWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRL 110
           MGTSSVE  TER LFTSLLSAV EWLLICMLFVDSIFSFFITKCA  WKL  PCLLCSRL
Sbjct: 1   MGTSSVEPRTERSLFTSLLSAVSEWLLICMLFVDSIFSFFITKCAQIWKLRTPCLLCSRL 60

Query: 111 DHVFSSEKRGYLWKLICGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKSNSET 170
           DH+F SEKRGYLWKLIC KH+ E+S LVLCHAHNKLVNVHEMCE+CLFSFATIN+SNSET
Sbjct: 61  DHIFGSEKRGYLWKLICSKHKLELSSLVLCHAHNKLVNVHEMCESCLFSFATINQSNSET 120

Query: 171 YRLLVGKLGEDPRHVIDRHPLLGDQKHDSLSEKHCSCCKEPYVSRAFVQKLIQTRSNGLE 230
           YRLLVGK+G+DP   IDR PLLG QK+D+LS+K CSCCKE YV R   Q LIQ RS+GLE
Sbjct: 121 YRLLVGKMGDDPYPGIDRDPLLGGQKYDTLSQKFCSCCKELYVPRGLGQSLIQARSSGLE 180

Query: 231 TKDLDVPLSSSVVHCKEDFQEASSNALPQVQY---------------------------- 290
            +DLDVPLSSSV HC+ED  E+SSN LP VQY                            
Sbjct: 181 AEDLDVPLSSSVAHCEEDCHESSSNPLPHVQYRELKIISDTESDGNGSILGIEMANSANS 240

Query: 291 -------------------------------------------------RELKITSDTES 350
                                                            RELKITSDTES
Sbjct: 241 KDDLTVQDVNMEPNFISLASNLTSTKLVELASAPEPLVLEPLVTPHVQHRELKITSDTES 300

Query: 351 DGYGSTLGVETANSKDDFTVQGVHMEPNLISLDSNLTSTKLVEPALAPEPLVIEPLVSLG 410
           DG GSTL VET NSKDD TVQGV+ EPN+ISL  NLTS KLV+PALAPEPL++EPLV L 
Sbjct: 301 DGNGSTLRVETTNSKDDLTVQGVNTEPNVISLAGNLTSMKLVDPALAPEPLILEPLVLLD 360

Query: 411 DALPPVECGVSIGHGLDELTPKHVEANGVFSSPTDLLSLDNMVSSSNTIATSIEALDESY 470
           + LPP+ECGVSIGHGLDELTPKHVE N VFSSPTD+L  D+M  SSNTIAT +E ++E+Y
Sbjct: 361 NPLPPIECGVSIGHGLDELTPKHVEVNEVFSSPTDILPPDSMAPSSNTIATPVEVVEENY 420

Query: 471 VTRNEEYETECRETEKAEILPTKATSEAGSEAQPVSSDAVQMAPNILEMGDAYKLALGAR 530
           VTR+EEYE E R TEKAEILPTKATSEAGSEAQPVSSDAVQMAPN+LE+GDAYKLA+G R
Sbjct: 421 VTRSEEYEMEIRGTEKAEILPTKATSEAGSEAQPVSSDAVQMAPNMLELGDAYKLAVGTR 480

Query: 531 GGRQLSGKLSEQWLERDSSRVSEDLKLLLSQLSFNRMNDQSRDMSPRLSINGDESRNFDV 590
           GGRQLSGKLSEQW+ ++SS+VSEDLKLLLSQLSFNR+NDQSRDMSPRLS+NGDE RNFD 
Sbjct: 481 GGRQLSGKLSEQWIGKESSKVSEDLKLLLSQLSFNRLNDQSRDMSPRLSVNGDELRNFDF 540

Query: 591 SSAVGMQMLQKRISLERNESGLESLDGSIVTEIEGENVVDRLKRQIEYDKRIMNSLYKEL 650
           SS  GMQMLQKR+SLERNESGLESLDGSIV+E+EGENVVDRLKRQ+EYD+++M+SLYKEL
Sbjct: 541 SS-TGMQMLQKRLSLERNESGLESLDGSIVSEVEGENVVDRLKRQVEYDEKLMSSLYKEL 600

Query: 651 DEERNASAIATNQAMAMITRLQEEKATLHMEALQCLRMMEEQNEYDDDALQKANDLISEK 710
           +EERNASAIA NQAMAMITRLQEEKA LHMEALQCLRMMEEQ+EYDD+ALQKANDLI+EK
Sbjct: 601 EEERNASAIAANQAMAMITRLQEEKANLHMEALQCLRMMEEQSEYDDEALQKANDLITEK 660

Query: 711 DKEIQDLEAELEFYRINFPNAYTIDNLVETSNVKERDIRVVHLESNQIGTIGYGNSVAGE 770
           DKEIQDLEAELEFYRINFPNAYTIDNLVETS VKERDIRVVHLESNQ GTIGYGN VAG+
Sbjct: 661 DKEIQDLEAELEFYRINFPNAYTIDNLVETS-VKERDIRVVHLESNQTGTIGYGNHVAGK 720

Query: 771 PDIHEKVGSEGSTYNNLLLEFEDEKLDIMQCLKMLENRLHLFSNNGDKIDLSNGEYF--- 830
           PDIHEKVGSEGSTYNNLLLEFEDEKL+I+QCLK LEN L+LFSNNG K+DLSNGEYF   
Sbjct: 721 PDIHEKVGSEGSTYNNLLLEFEDEKLNIVQCLKKLENMLYLFSNNGVKMDLSNGEYFGTE 780

Query: 831 GDFDSDNRKLEDGKDHACLPGKDLHDDDDRLPSLANPSLSKESSELDCSDRNSLLATGMA 871
           G F S    L+        PG+D H +DDRLPS+ N S  KESSELD SDRNSLLAT MA
Sbjct: 781 GSFPSGTNDLDLDNSKQKTPGEDAHANDDRLPSVTNSSFDKESSELDYSDRNSLLATEMA 840

BLAST of Spg003486 vs. NCBI nr
Match: KAA0063582.1 (myosin-binding protein 1-like [Cucumis melo var. makuwa] >TYJ97909.1 myosin-binding protein 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1227.2 bits (3174), Expect = 0.0e+00
Identity = 658/906 (72.63%), Postives = 722/906 (79.69%), Query Frame = 0

Query: 51  MGTSSVEAHTERDLFTSLLSAVFEWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRL 110
           MGTSSVEA TER LFT+L+SAVFEWLLICMLF DSIFSFFITKCAHFWKL  PCLLCSRL
Sbjct: 1   MGTSSVEARTERSLFTALVSAVFEWLLICMLFADSIFSFFITKCAHFWKLCTPCLLCSRL 60

Query: 111 DHVFSSEKRGYLWKLICGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKSNSET 170
           DH+F SEKRGY+W LIC KH+ E+S LVLCHAHNKLVNVHEMCENCLFSFAT NKSNSET
Sbjct: 61  DHIFGSEKRGYIWNLICSKHKLELSSLVLCHAHNKLVNVHEMCENCLFSFATFNKSNSET 120

Query: 171 YRLLVGKLGEDPRHVIDRHPLLGDQKHDSLSEKHCSCCKEPYVSRAFVQKLIQTRSNGLE 230
           YRLLVGKLG+DP   IDR PLLGDQK+D+LS+K CSCCKE YV R F Q LIQTRS+GLE
Sbjct: 121 YRLLVGKLGKDPYPRIDRDPLLGDQKYDTLSQKCCSCCKELYVPRGFAQSLIQTRSSGLE 180

Query: 231 TKDLDVPLSSSVVHCKEDFQEASSNALPQVQYRELKITS--------------------- 290
            +DLDVPL+SS VH   D QE SSN  P VQYRELKITS                     
Sbjct: 181 AEDLDVPLASSAVHYDGDSQECSSNPRPHVQYRELKITSDTESEGNGSILGVERANSLKD 240

Query: 291 -------------------------------------------------------DTESD 350
                                                                  DTESD
Sbjct: 241 DLTIQDVNMEPNFISLASNLTSTKLIEPALAPEPLVLEPLATTPYVQNRELKITPDTESD 300

Query: 351 GYGSTLGVETANSKDDFTVQGVHMEPNLISLDSNLTSTKLVEPALAPEPLVIEPLVSLGD 410
           G GSTL VET NSKDD TV GV+ EPN+ISLDSNLTS KLVEPALAPEPLV+EPLVSL D
Sbjct: 301 GNGSTLRVETTNSKDDLTVLGVNTEPNIISLDSNLTSAKLVEPALAPEPLVLEPLVSLDD 360

Query: 411 ALPPVECGVSIGHGLDELTPKHVEANGVFSSPTDLLSLDNMVSSSNTIATSIEALDESYV 470
           ALPP ECGV IGHGLDE+TPK VE NG FSSP+DLL +DN+VSSSNT  T +EA++ES V
Sbjct: 361 ALPPAECGVLIGHGLDEVTPKLVEVNGFFSSPSDLLPIDNVVSSSNTKETPVEAVEESCV 420

Query: 471 TRNEEYETECRETEKAEILPTKATSEAGSEAQPVSSDAVQMAPNILEMGDAYKLALGARG 530
            R+EEYE E R TEKAEILPTKATSEAGSE QPVSSD+ QM PN+LE+GDAYKLA+GARG
Sbjct: 421 ERSEEYEKESRGTEKAEILPTKATSEAGSEVQPVSSDSAQMVPNMLELGDAYKLAVGARG 480

Query: 531 GRQLSGKLSEQWLERDSSRVSEDLKLLLSQLSFNRMNDQSRDMSPRLSINGDESRNFDVS 590
           GRQLSGKL EQW+ ++SS+VSEDLKLLL+QLSFNR+NDQSR+MSPRLS+NGDE RNFD  
Sbjct: 481 GRQLSGKLLEQWIGKESSKVSEDLKLLLTQLSFNRLNDQSREMSPRLSVNGDEVRNFDYL 540

Query: 591 SAVGMQMLQKRISLERNESGLESLDGSIVTEIEGENVVDRLKRQIEYDKRIMNSLYKELD 650
           SAVGMQMLQKR SLERNESG+ESLDGSI++EI+GEN+ DRLKRQIEYDK++M+SLYKEL+
Sbjct: 541 SAVGMQMLQKRCSLERNESGVESLDGSIISEIDGENMADRLKRQIEYDKKVMSSLYKELE 600

Query: 651 EERNASAIATNQAMAMITRLQEEKATLHMEALQCLRMMEEQNEYDDDALQKANDLISEKD 710
           EERNASAIA NQAMAMITRLQEEKA LHMEAL CLRMMEEQ+EYDDDALQKANDLI+EKD
Sbjct: 601 EERNASAIAANQAMAMITRLQEEKANLHMEALHCLRMMEEQSEYDDDALQKANDLITEKD 660

Query: 711 KEIQDLEAELEFYRINFPNAYTIDNLVETSNVKERDIRVVHLESNQIGTIGYGNSVAGEP 770
           KEIQDLEAELEFYRINFPNAYTIDNLVETS VKERDIRVVHLESNQI  IG  N V G+P
Sbjct: 661 KEIQDLEAELEFYRINFPNAYTIDNLVETS-VKERDIRVVHLESNQIEPIGNRNLVTGKP 720

Query: 771 DIHEKVGSEGSTYNNLLLEFEDEKLDIMQCLKMLENRLHLFSNNGDKIDLSNGEYFG--- 830
           D+HE+VGSEGSTYN LLLEFEDEKL+I Q LK LEN LHLFSN+G K+DLSNG+YFG   
Sbjct: 721 DLHEEVGSEGSTYNILLLEFEDEKLNIKQHLKKLENMLHLFSNDGVKMDLSNGKYFGNKR 780

Query: 831 -------DFDSDNRKLEDGKDHACLPGKDLHDDDDRLPSLANPSLSKESSELDCSDRNSL 871
                  D D D+RKLED + HACLPG+D H +DD LPSL N S  KESSELDCSD+NS 
Sbjct: 781 SFSSGTNDLDLDDRKLEDREHHACLPGEDAHIEDDHLPSLTNSSFDKESSELDCSDKNSP 840

BLAST of Spg003486 vs. NCBI nr
Match: XP_022946503.1 (myosin-binding protein 1-like [Cucurbita moschata] >XP_022946504.1 myosin-binding protein 1-like [Cucurbita moschata])

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 621/860 (72.21%), Postives = 686/860 (79.77%), Query Frame = 0

Query: 51  MGTSSVEAHTERDLFTSLLSAVFEWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRL 110
           MGT SVEA T   L T LLSAV E LLICMLF+ SIFSFFITKCA  WKL  PCLLCSRL
Sbjct: 1   MGTLSVEACTGGSLRTYLLSAVSECLLICMLFLHSIFSFFITKCARLWKLRIPCLLCSRL 60

Query: 111 DHVFSSEKRGYLWKLICGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKSNSET 170
           DHVF SEK+GYLW LICGKH+ E+S LVLCHAHNKLVNVHEMCENCLFSFATINK NSET
Sbjct: 61  DHVFGSEKKGYLWTLICGKHKLEVSSLVLCHAHNKLVNVHEMCENCLFSFATINKLNSET 120

Query: 171 YRLLVGKLGEDPRHVIDRHPLLGDQKHDSLSEK--------------------------- 230
           YRLLVGKLGEDP   ID  P LGDQKH S  E+                           
Sbjct: 121 YRLLVGKLGEDPHFGIDSDPSLGDQKHCSCCEESYIPRGFVQTLIQTRSSGLKVEDLNVP 180

Query: 231 ------------HCSCCKEPYVSRAFVQKLIQTRSNGLETKDLDVPLSSSVVHCKEDFQE 290
                         SCC+E YV R FVQ  IQTRS+GL+ +DLDVPLSSS+V CK D Q 
Sbjct: 181 LSSSIVPCKVDVQGSCCEESYVPRGFVQTSIQTRSSGLKAEDLDVPLSSSIVPCKVDIQG 240

Query: 291 ASSNALPQVQYRELKITSDTESDGYGSTLGVETANSKDDFTVQGVHMEPNLISLDSNLTS 350
             SN LP VQY+EL ITSDTESDG G TL VETANSKDD  +Q  +ME N  SL SNLTS
Sbjct: 241 PPSNPLPHVQYKELNITSDTESDGNGGTLVVETANSKDDLALQDDNMEANFSSLASNLTS 300

Query: 351 TKLVEPALAPEPLVIEPLVSLGD-ALPPVECGVSIGHGLDELTPKHVEANGVFSSPTDLL 410
           T LVEPALAPEPLV+     LGD ALP VE GVSIGHGLDE TPKHVEAN  FSSP DLL
Sbjct: 301 TTLVEPALAPEPLVL-----LGDAALPRVERGVSIGHGLDESTPKHVEANDGFSSPNDLL 360

Query: 411 SLDNMVSSSNTIATSIEALDESYVTRNEEYETECRETEKAEILPTKATSEAGSEAQPVSS 470
           SLDNMV SSNTI TS+EA++ESYVTR+EE+ET+ R TEKAEI PTKATSE  +E QPVSS
Sbjct: 361 SLDNMVPSSNTIVTSVEAVEESYVTRSEEHETKSRGTEKAEISPTKATSEEDTETQPVSS 420

Query: 471 DAVQMAPNILEMGDAYKLALGARGGRQLSGKLSEQWLERDSSRVSEDLKLLLSQLSFNRM 530
           D  QMAPN LE+GDAYK+A+GAR GRQLSGKLSEQW+E+DSS+VS+DLKLL++QLSFNR 
Sbjct: 421 DTAQMAPNTLELGDAYKIAVGARAGRQLSGKLSEQWIEKDSSKVSDDLKLLMTQLSFNRT 480

Query: 531 NDQSRDMSPRLSINGDESRNFDVSSAVGMQMLQKRISLERNESGLESLDGSIVTEIEGEN 590
            DQSR+MSPRLSINGD     DVS+ VGMQ+ QKRISLERNES L+SLDGSIV+EIEGEN
Sbjct: 481 TDQSREMSPRLSINGD-----DVSNIVGMQIPQKRISLERNESSLDSLDGSIVSEIEGEN 540

Query: 591 VVDRLKRQIEYDKRIMNSLYKELDEERNASAIATNQAMAMITRLQEEKATLHMEALQCLR 650
           VVDRLKRQ+EYDK++M SLYKEL+EERNASAIA NQAMAMITRLQEEKA LHMEALQCLR
Sbjct: 541 VVDRLKRQVEYDKKLMISLYKELEEERNASAIAANQAMAMITRLQEEKANLHMEALQCLR 600

Query: 651 MMEEQNEYDDDALQKANDLISEKDKEIQDLEAELEFYRINFPNAYTIDNLVETSNVKERD 710
           MMEEQ+EYDDDALQKANDLI+EKDKEIQDLEAELEF+RINFPNAYTIDNL+E S VKERD
Sbjct: 601 MMEEQSEYDDDALQKANDLITEKDKEIQDLEAELEFFRINFPNAYTIDNLIEMS-VKERD 660

Query: 711 IRVVHLESNQIGTIGYGNSVAGEPDIHEKVGSEGSTYNNLLLEFEDEKLDIMQCLKMLEN 770
           I VVHLESNQ+GTIGYGN V G+PDIHEKVGSEG T+NNLL EFEDEK++I+QCL+ LEN
Sbjct: 661 IGVVHLESNQLGTIGYGNLVVGKPDIHEKVGSEGGTFNNLLSEFEDEKINILQCLQKLEN 720

Query: 771 RLHLFSNNGDKIDLSNGEYFGDFDSDNRKLEDGKDHACLPGKDLHDDDDRLPSLANPSLS 830
            +HLFS NG K+DLSNGEY G   +D+ KLE+G+DHA     D +D+DD LPSLANP   
Sbjct: 721 MIHLFSINGVKMDLSNGEYLG---TDDLKLENGEDHA-KDDDDDNDNDDCLPSLANPPFD 780

Query: 831 KESSELDCSDRNSLLATGMADFASLRNEMSNLNKRMEVLETDKNFLEHTINSLKKGEEGL 871
           KES+ELD SDRNS   T   DFA LR E+S LNKRMEVLE DKN LEHTINSL +GEEGL
Sbjct: 781 KESNELDRSDRNSSSNTEREDFAFLRIEVSKLNKRMEVLEADKNILEHTINSLIRGEEGL 840

BLAST of Spg003486 vs. ExPASy Swiss-Prot
Match: F4HXQ7 (Myosin-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=MYOB1 PE=1 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 5.4e-71
Identity = 331/1213 (27.29%), Postives = 460/1213 (37.92%), Query Frame = 0

Query: 65   FTSLLSAVF-EWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRLDHVFSSEK--RGY 124
            FT  L+  F EWLL+ MLFV+SIFS+ I + A + +L +PCL+CS LDH+    K  +  
Sbjct: 6    FTRALALAFNEWLLMFMLFVNSIFSYVIARFADYSELQSPCLMCSNLDHILRRTKDLKKT 65

Query: 125  LWKLICGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKSNSETYRLLVGKLGED 184
             W +IC KH+ EIS LV CHAH KLV+V  MCE CLFSFAT NKSN+ETYRLLVGKLGED
Sbjct: 66   HWDIICSKHKSEISSLVYCHAHGKLVDVRGMCETCLFSFATTNKSNAETYRLLVGKLGED 125

Query: 185  PR--HVIDRHPLLGDQKHDSLSE-KHCSCCKEPY---------VSRAFVQKL-----IQT 244
                   DR       K+ + S+   C+CC + +           R  + K+     I+T
Sbjct: 126  SHFGSKSDR------SKYPNCSKLTDCTCCNQLWTPQTAATQVAEREILPKIGLLGKIRT 185

Query: 245  RSNGLETKDL------DVPLSSSVVHCKEDFQ---------------------------- 304
                   K +      DV  +   +H   + +                            
Sbjct: 186  GKQSAPKKSVSFNHLPDVGYTELKIHSDTESEAVFSDTEPKQESSLNHLPPVGYNEPKIG 245

Query: 305  ---------------EASSNALPQVQYRELKITSDTESDGYGS----------------- 364
                             S N LP V Y ELKI SDTES+   S                 
Sbjct: 246  LVGDVRTGKPSTPKKSVSFNHLPDVGYTELKIHSDTESEAVFSEDECVVLKDEDHKYQIV 305

Query: 365  --------TLGVETANSK---DDF------------------------------------ 424
                    TL  + A  K    DF                                    
Sbjct: 306  DLQTHPIITLPYDLATDKLLNFDFPLEPFVTRNDREEVQLQETNWRTYSSFPVLIPVNDV 365

Query: 425  --TVQGVHMEPNLISLD------------------------------------------- 484
              T + V  E  + SLD                                           
Sbjct: 366  PETSEKVFKEEEINSLDNLFLTSRAMKHFAAAKVKEEPIRLQDISSTPDVKENPANASLM 425

Query: 485  --------SNLTSTK----------------------LVEPALAPEPL------------ 544
                    S++T+T                        V P     P             
Sbjct: 426  EETELICLSDVTATSGAMEHSEVILKEREELIHLQDISVTPDFKENPANASLLEETELIC 485

Query: 545  ---VIEPL-------VSLGDALPPV----------------------------------E 604
               V  PL       V L D + P+                                   
Sbjct: 486  LNDVTSPLRAVEHSAVLLKDKVEPIRLQDGGSLTPDFMENSANASILEETELICVNDVTS 545

Query: 605  CGVSIGHGL--------------DELTPKHVE---------------------------- 664
               ++GH                  LTP   E                            
Sbjct: 546  TSRTMGHSSVVLKENEEPIRFQDSSLTPDFKENPASTFLVEETELICLNDVTSPSRAMEH 605

Query: 665  ---------------------------ANGVFSSPTDL---------------------- 724
                                       AN      T+L                      
Sbjct: 606  STVFIEEKEELVRHQNITLTQDFMENPANSSLREETELICLNDVTSTSEVAETPEDVLEG 665

Query: 725  --------LSLDNMVSSSNTIATSIE-------------ALDESYVT------------- 784
                    +SLD +  S  T  TS+E             +L+  Y+              
Sbjct: 666  IELMSIHDISLDEVSESVTTNQTSVEISKERDTDQADITSLESEYIVVPSPNSMPENSTD 725

Query: 785  -----RNEEYETECRETEKAEILPTKATS--EAGSEAQ-------PVSSDAVQMAPNILE 844
                 + E  ET  R +  +E+ P   TS  EA  E++        V+++  Q +  +L+
Sbjct: 726  NCVSDKKEMKETSLRISSLSEMAPRDVTSHTEAALESESSSFNSMSVAAETNQYSGELLD 785

Query: 845  MGDAYKLALGARGGRQLSGKLS-EQWLERDSSRVSEDLKLLLSQLSFNRMND--QSRDMS 870
            + DAY + +G  G    +G+   E W+++D+SRVSEDLK LL+Q+S +R  +    RD+S
Sbjct: 786  LADAYNIVVGNEGHYDSNGRQQIENWMKKDTSRVSEDLKALLTQISASRGIEFLSPRDVS 845

BLAST of Spg003486 vs. ExPASy Swiss-Prot
Match: F4INW9 (Probable myosin-binding protein 4 OS=Arabidopsis thaliana OX=3702 GN=MYOB4 PE=3 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 5.0e-48
Identity = 203/720 (28.19%), Postives = 315/720 (43.75%), Query Frame = 0

Query: 68  LLSAVFEWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRLDHVFSSEKRGYLWK-LI 127
           L  A  EW LI ++F+D++ S+ +   A + +L  PC LCS+L H          W+ L+
Sbjct: 19  LTYAACEWFLILLMFIDALLSYLLVWFARYCRLQMPCFLCSKLLHPLH-------WRFLL 78

Query: 128 CGKHRQEISCLVLCHAH-NKLVNVHEMCENCLFSFATINKSNSETYRLLVGKLGEDPRHV 187
           C  HR E+S  + C  H N L +   MC++CL SF  +   N +  RLL+GKLG D    
Sbjct: 79  CRNHRSEVSSYMSCQNHGNNLADCRGMCDDCLLSFTKMTGPNPDMNRLLLGKLGYD---- 138

Query: 188 IDRHPLLGDQKHDSLSEKHCSCCKEPYVSRAFVQKLIQTRSNGLETKDL-DVPLSSSVVH 247
                 L  + H +   + CSCC +P+ +R   Q+LI+  S G  +    ++P    +  
Sbjct: 139 ------LLSRSHFA-HPRSCSCCNKPWRTRHHTQRLIRLGSRGRNSSSKPNIPAPRHLTR 198

Query: 248 -----------------CKEDFQEASSNALPQVQYRELKITSDTESDGYGS--------- 307
                              E     S + +  V Y ELKI SD+ES+   S         
Sbjct: 199 RGSGGSLKKMRDHIATSGSEYVDVGSRDGMAHVGYTELKIHSDSESEFLFSDDDAFLHIT 258

Query: 308 TLGVETANSK-----------------------DDFTVQGVHMEPNLISLDSNL-----T 367
              VE +  +                        D   + +H+E N     S L      
Sbjct: 259 DFNVEPSEKRTHKSRRRKSFDDKKMSNHKQPVLQDNQYKKIHVEDNETVESSMLGYNLEN 318

Query: 368 STKLVEPALAPE-PLVIEPLVSLGDALP-----PVECGVSI---GHGLDELTPKHVEANG 427
            T+  +P  A E   V+  L+++ +A P     P +    +        E++     + G
Sbjct: 319 RTRQKQPVKAKEHDDVLSELITMSEARPFLLGSPRKYAAGVVTQNENEAEVSGSSSPSGG 378

Query: 428 VFSSPTDLLSLDNMVSSSNTIATSIEALD-----ESYVTRNEEYET--ECRETEKAEILP 487
            F SP    S +N  S    I    ++ D      S     EE+E   E +E++  ++  
Sbjct: 379 EFLSP----SAENGASREIRIQEHDDSSDFSQNITSSAMEIEEFEAAIEQKESDHMDVSG 438

Query: 488 TKATSEAGSEAQPVSSDAVQMAPNILEMGDAYKLALGARGGRQLS-GKLSEQWL----ER 547
           + A   +  E   V  D+  +  N   M D+ +         +++   ++E++     E 
Sbjct: 439 SVANEPSSDEENEVEGDSKPLISN--NMSDSLEQEQSGEEESEVNENNVAEEYFSNEEED 498

Query: 548 DSSRVSEDLKLLLSQLSF-NRMNDQSRDMSPRLSINGDESRN-FDVSSAVGMQMLQKRI- 607
           + +  +E L       SF    + +  D S   S+  D S N  DV +     M    + 
Sbjct: 499 EVNGHTEPLTSKSESGSFAEEQSSEDEDGSNIYSVAKDHSSNEEDVDNEESEPMTSNNVT 558

Query: 608 -------------------------SLERNESGLESLDG--------------------- 654
                                    ++ + E  LE  D                      
Sbjct: 559 GVVKEEHSAKEEHGDHEETEPLTSLNISKEEPSLEHSDKDSLKITETRNTSNGSPELKHS 618

BLAST of Spg003486 vs. ExPASy Swiss-Prot
Match: Q9LMC8 (Probable myosin-binding protein 5 OS=Arabidopsis thaliana OX=3702 GN=MYOB5 PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 1.1e-34
Identity = 176/724 (24.31%), Postives = 279/724 (38.54%), Query Frame = 0

Query: 51  MGTSSVEAHTERDLFT---SLLSAVFEWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLC 110
           M   S +   E +L T    L+ A+ EW+LI +LF+D   +FF  + A F+ L  PCLLC
Sbjct: 1   MSNRSFKNFIEEELGTFPQFLIYALLEWILIIILFIDGFLAFFSNQIAKFFDLKIPCLLC 60

Query: 111 SRLDHVFSSEKRGYLWK-LICGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKS 170
           +RLDHV  S    + +   IC  H++ +S L  CH H KL  +  MCE CL SFAT  ++
Sbjct: 61  TRLDHVLVSRNPDFYYNDSICDAHKKNVSSLAYCHVHKKLSEIKRMCEGCLLSFATEKET 120

Query: 171 NSETYRLLVGKLGEDPRHVID-------RHPLLGDQKHDSLSEKHCSCCKEPYVSRAFVQ 230
           + +TY+ L+G L +D   +ID         P+ G +K ++  +                 
Sbjct: 121 DVDTYKSLIGILHKDLELLIDDERELQLAFPVAGSKKDENFYQ----------------- 180

Query: 231 KLIQTRSNGLETKDLDVPLSSSVVHCKEDFQ-EASSNALPQVQYRELKITSDTESDGYGS 290
             ++ R+N                +  + FQ +   +   Q+    +K+ SD       S
Sbjct: 181 --VENRTN----------------NSNDRFQRQQRCSCCGQI----MKLKSDKPKSNNQS 240

Query: 291 TLGVETANSKDDFTVQGVHMEPNLISLD-SNLTSTKLVEPALAPEPLVIEPLVSLGDALP 350
             G  + + +  F         N  +LD SN+  T L E                 DAL 
Sbjct: 241 FFGAPSPSPRVSF---------NQRTLDLSNIKYTDLPED---------------DDAL- 300

Query: 351 PVECGVSIGHGLDELTPKHVEANGVFSSPTDLLSLDNMVSSSNTIATSIEALDESYVTRN 410
                                                     NT   S++A+D+      
Sbjct: 301 ------------------------------------------NTKGASLDAVDD------ 360

Query: 411 EEYETECRETEKAEILPTKATSEAGSEAQPVSSDAVQMAPNILEMGDAYKLALGARGGRQ 470
                                                  P+ ++ G+ +           
Sbjct: 361 -------------------------------------RTPSFVKGGNKF----------- 420

Query: 471 LSGKLSEQWLERDSSRVSEDLKLLLSQLSFNRMNDQSRDMSPRLSINGDESRNFDVSSAV 530
                                        F      S   SPR S+   +    D     
Sbjct: 421 -----------------------------FGIPLSDSAQNSPRWSVRSMKKSFVD----- 480

Query: 531 GMQMLQKRISLERNESGLESLDGSIVTEIEGENVVDRLKRQIEYDKRIMNSLYKELDEER 590
                         ++GLES        ++G++++  L RQ+  D++ +  LY ELDEER
Sbjct: 481 --------------QNGLES------EVLDGDSILQHLNRQVRLDRKSLMDLYMELDEER 501

Query: 591 NASAIATNQAMAMITRLQEEKATLHMEALQCLRMMEEQNEYDDDALQKANDLISEKDKEI 650
           +ASA+A N AMAMITRLQ EKA + MEALQ  RMM+EQ EYD +ALQ  N L+ ++++E+
Sbjct: 541 SASAVAANNAMAMITRLQAEKAAVQMEALQYQRMMDEQAEYDQEALQSMNGLLVKREEEM 501

Query: 651 QDLEAELEFYRINFPNAYTIDNLVETSNVKERDIRVVHLESNQIGTIGYGNSVAGEPDIH 710
           ++LEA +E YR+ +        L+     +  +   +  E+  +  +   +S   E    
Sbjct: 601 KELEAGIEVYRLRY-------GLLREERGEAEEF--LDEETKPVSDLPVCSSNHEEDLEQ 501

Query: 711 EKVGSEGSTYNNLLLEFEDEK-------------LDIMQCLKMLENRLHLFSNNGDKIDL 749
            K  +E S  NN ++  E+EK              +I + L  +E++  L     D +D+
Sbjct: 661 MKDSAEDSIGNNGVMIIEEEKENGSRKDMLVKEISEITERLNAIESKGELLQQISDVLDV 501

BLAST of Spg003486 vs. ExPASy Swiss-Prot
Match: F4HVS6 (Probable myosin-binding protein 6 OS=Arabidopsis thaliana OX=3702 GN=MYOB6 PE=2 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 3.8e-32
Identity = 174/700 (24.86%), Postives = 266/700 (38.00%), Query Frame = 0

Query: 68  LLSAVFEWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRLDHVF-SSEKRGYLWKLI 127
           L+  V EW LI  LF+D + +F   + A F+ L+ PCLLC+R+DH+    + + Y  + I
Sbjct: 40  LIYTVLEWSLIVFLFIDGVIAFLSNQFAKFFDLNIPCLLCTRIDHILVPRDPQFYYNESI 99

Query: 128 CGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKSNSETYRLLVGKLGEDPRHVI 187
           C  H++++S L  CH H KL  +  MCE CL SFAT   S+ +TY+ L+G L +D   +I
Sbjct: 100 CDSHKKKVSSLAYCHVHKKLSEIKHMCEGCLLSFATEKDSDCDTYKSLIGILHKDLELLI 159

Query: 188 DRHPLLGDQKHDSLSEKHCSCCKEPYVSRAFVQKLIQTRSNGLETKDLDVPLSSSVVHCK 247
           D      D++   L+ K                 L+QT  N ++ K  ++   S   HC 
Sbjct: 160 D------DERDLPLAFKK-------------DDNLVQTTKNLVDYKTNNIKNDSLKQHC- 219

Query: 248 EDFQEASSNALPQVQYRELKITSDTESDGYGSTLGVETANSKDDFTVQGVHMEPNLISLD 307
                +    L       LKI S+       S L                          
Sbjct: 220 -----SCCGEL-------LKIKSEKLPKNNNSFL-------------------------- 279

Query: 308 SNLTSTKLVEPALAPEPLVIEPLVSLGDALPPVECGVSIGHGLDELTPKHVEANGVFSSP 367
                      A AP P V                                         
Sbjct: 280 -----------APAPSPRV----------------------------------------- 339

Query: 368 TDLLSLDNMVSSSNTIATSIEALDESYVTRNEEYETECRETEKAEILPTKATSEAGSEAQ 427
                      S N ++               E E+E ++ +                  
Sbjct: 340 -----------SHNKLS---------------ENESEFKDMD------------------ 399

Query: 428 PVSSDAVQMAPNILEMGDAYKLALGARGGRQLSGKLSEQWLERDSSRVSEDLKLLLSQLS 487
                 V   P+ +            RGG +  G                   + LS  +
Sbjct: 400 ------VDRTPSFV------------RGGNKFFG-------------------IPLSDSA 459

Query: 488 FNRMNDQSRDMSPRLSINGDESRNFDVSSAVGMQMLQKRISLERNESGLESLDGSIVTEI 547
            N         SPR S+                    K+  L + E+  ++ D +     
Sbjct: 460 QN---------SPRWSVRS-----------------LKKSVLNKTENASDTTDPT----- 516

Query: 548 EGENVVDRLKRQIEYDKRIMNSLYKELDEERNASAIATNQAMAMITRLQEEKATLHMEAL 607
            GE+++++LK+++  DK+ +  LY ELDEER+ASA+A N+AMAMITRLQ EKA + MEAL
Sbjct: 520 -GESILNQLKKEVRLDKKSLIDLYMELDEERSASAVAANEAMAMITRLQAEKAAVQMEAL 516

Query: 608 QCLRMMEEQNEYDDDALQKANDLISEKDKEIQDLEAELEFYRINF--------------- 667
           Q  RMM+EQ EYD +ALQ  +  ++++++E+++LEAE E YR  +               
Sbjct: 580 QYQRMMDEQAEYDQEALQSMSSELAKREEEMKELEAEFEVYREKYGCLTDQEDAREEFHK 516

Query: 668 --PNAYTIDNLVETSNVKERDIRVVHLESNQIGTIGYGNSVAGEPDIHEKVGSEGSTYNN 727
              NA   D+  ET  V +  +   + + N       G S   E    E V S      +
Sbjct: 640 QNGNASAYDDCQETKPVSDLAVSSSNQQENGENIDQNGQSKRSEESTAENVVSADEEKGS 516

Query: 728 LLLE-FEDEKLDIMQCLKMLENRLHLFSNNGDKIDLSNGE 749
              E    E  +I + L  L++   L  +  D +D+S GE
Sbjct: 700 ESKEGIVKELSEITERLSTLQSNGDLLKHIADVLDVSEGE 516

BLAST of Spg003486 vs. ExPASy Swiss-Prot
Match: Q0WNW4 (Myosin-binding protein 3 OS=Arabidopsis thaliana OX=3702 GN=MYOB3 PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 1.2e-30
Identity = 201/818 (24.57%), Postives = 336/818 (41.08%), Query Frame = 0

Query: 68  LLSAVFEWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRLDHVF--SSEKRGYLWKL 127
           L+ A  EWLL+  +F++S F++FI K A F+ L   CLLC +LD +F    E R    +L
Sbjct: 21  LVYAFLEWLLMFFIFLNSFFTYFIVKFASFFGLKQVCLLCPKLDRIFERKPENRFTYKEL 80

Query: 128 ICGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKSNSETYRLLVGKLGEDPRHV 187
           +C  H  E++ L  C  H KL     +C +C         SN E                
Sbjct: 81  LCQNHIAELASLSFCRTHGKLSESANLCSDC---------SNRE---------------- 140

Query: 188 IDRHPLLGDQKHDSLSEKHCSCCKEPYVSRAFVQKLIQTRSNGLETKDLDVPLSSSVVHC 247
                    ++  ++    C+CC++    + +   L+               L SS+   
Sbjct: 141 ---------EEQSNIGLGFCTCCQKSLADKPYPNYLL---------------LKSSI--- 200

Query: 248 KEDFQEASSNALPQVQYRELKITSDTESDGYGSTLGVETANSKDDFTVQGVHMEPNLISL 307
                                         +G TLG        D    G+ +E     +
Sbjct: 201 ------------------------------WGKTLG--------DREDGGLILE----MI 260

Query: 308 DSNLTSTKLVEPALAPEPLVIEPLVSLGDALPPVECGVSIGHGLDELTPKHVEANGVFSS 367
           D +                        GD           G  +D    +     G F  
Sbjct: 261 DDD----------------------KFGD-----------GFEID----RESYPLGFFRD 320

Query: 368 PTDLLSLDNMVSSSNTIATSIEALDESYVTRNEEYETECRETEKAEILPTKATSEA-GSE 427
             +     +   +   I+              E Y    RE  + + L +  ++ + G+E
Sbjct: 321 KAEEGKKQDQQQNGEVISDV------------ESYGLSLREVSEEDGLRSIISNNSPGNE 380

Query: 428 AQPVSSDAVQMAPNILEMGDAYKLALGARGGRQLSGKLSEQWLERDSSRVSEDLKLLLSQ 487
           A+   S+  Q   +           +   G  Q+SG++ E   + + + V++   LL  Q
Sbjct: 381 AKSRVSEDEQRNDDTSN--------VATYGEDQISGRVEE---KEEETGVAD---LLYDQ 440

Query: 488 LSFNRMNDQSRDMSPRLSINGDESRNFDVSSAVGMQML--QKRISLERNE--SGLESLDG 547
             F   N     +        + ++  D  +   +  L  +K   L RNE  +  ++ DG
Sbjct: 441 --FESKNFTGSQIEEEEEDREETTKELDPETPTSVSTLFNKKLHFLARNEYAAAEDAGDG 500

Query: 548 SI-VTEIEGEN---VVDRLKRQIEYDKRIMNSLYKELDEERNASAIATNQAMAMITRLQE 607
           ++ V+E++G +    ++RL+  +  ++  +  LY EL+EER+ASAI+ NQ MAMITRLQE
Sbjct: 501 NVLVSEMDGGDPLRTIERLRETVRAEQEALRDLYAELEEERSASAISANQTMAMITRLQE 560

Query: 608 EKATLHMEALQCLRMMEEQNEYDDDALQKANDLISEKDKEIQDLEAELEFYRINF--PNA 667
           EKA + MEALQ  RMMEEQ EYD +ALQ  N L+ +++KE + L+ ELE YR       +
Sbjct: 561 EKAKVQMEALQYQRMMEEQAEYDQEALQLLNHLMVKREKEKEQLQRELEVYRAKVLEYES 620

Query: 668 YTIDNLVETSNVKERDIRVVHLESNQIGTIGYGNSVAGEPDIHEKVG----SEGSTYNNL 727
              + ++   N  E D      E+ +       NS   + D+ EK+        S     
Sbjct: 621 KAKNKIIVVENDCEADDDDKEEENRE-----EDNSSEMDVDL-EKITLDCVQHMSMLGES 663

Query: 728 LLEFEDEKLDIMQCLKMLENRLHLFSNNGDKIDLSNGEYFGDFDSDNRKLEDGKDHACLP 787
           L EFE+E+L I+  LK+LE+RL          D  + E  G+F +   +  +G  H  L 
Sbjct: 681 LSEFEEERLVILDQLKVLEDRLVTMQ------DKESAEDPGEFSNSYEEASNG--HGGLT 663

Query: 788 GKDLHDDDDRLPSLANPSLSKESSELDCSDRNSLLATGMADFASLRNEMSNLNKRMEVLE 847
              +      L   A       S  L  SD  +  +   ++   +  ++ ++ +R++ LE
Sbjct: 741 MASMAKSLLPLLDAAENESEDGSQGLPESDEKNFGSD--SEKLEIIKQVDSVYERLQELE 663

Query: 848 TDKNFLEHTINSLKKGEEGLQFVQEIASHLQELRKIRI 869
           TD  FL++ ++S KKG++G   +++I  HL++LR I +
Sbjct: 801 TDGEFLKNCMSSAKKGDKGTDILKDILQHLRDLRTIEL 663

BLAST of Spg003486 vs. ExPASy TrEMBL
Match: A0A0A0LG67 (GTD-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G010190 PE=4 SV=1)

HSP 1 Score: 1273.8 bits (3295), Expect = 0.0e+00
Identity = 677/905 (74.81%), Postives = 737/905 (81.44%), Query Frame = 0

Query: 51  MGTSSVEAHTERDLFTSLLSAVFEWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRL 110
           MGTSSVEA +ER LFTSLLSAV EWLLICMLFVDSIFSFFITKCAHFWKLH PCLLCSRL
Sbjct: 1   MGTSSVEARSERSLFTSLLSAVSEWLLICMLFVDSIFSFFITKCAHFWKLHTPCLLCSRL 60

Query: 111 DHVFSSEKRGYLWKLICGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKSNSET 170
           DH+F SEKRGY+W LIC KH+ E+S LVLCHAHNKLVNVHEMCENCLFSFAT  KSNSET
Sbjct: 61  DHIFGSEKRGYVWNLICSKHKVELSSLVLCHAHNKLVNVHEMCENCLFSFATFKKSNSET 120

Query: 171 YRLLVGKLGEDPRHVIDRHPLLGDQKHDSLSEKHCSCCKEPYVSRAFVQKLIQTRSNGLE 230
           YRLLVGKLGEDP   IDR PLL DQK+D+ S+K CSCCKE YV R F Q LIQTRS+GLE
Sbjct: 121 YRLLVGKLGEDPYPGIDRDPLLADQKYDTSSQKCCSCCKELYVPRGFAQSLIQTRSSGLE 180

Query: 231 TKDLDVPLSSSVVHCKEDFQEASSNALPQVQYRELKITSDTESDGYGSTLGVETANS--- 290
            +DLDVPLSSS VHC+EDFQ++SSN LP VQYRELKITSDTES+G GS LGVE ANS   
Sbjct: 181 AEDLDVPLSSSAVHCEEDFQDSSSNPLPHVQYRELKITSDTESEGNGSILGVEAANSLKD 240

Query: 291 ------------------------------------------------------------ 350
                                                                       
Sbjct: 241 DLTIQDVNMEPNFISLASNLTSTKLMEPALAPEPFVLEPLLTPYVQNRELKINPDTESDG 300

Query: 351 ------------KDDFTVQGVHMEPNLISLDSNLTSTKLVEPALAPEPLVIEPLVSLGDA 410
                       KDD TVQGV  EPN+I+LDSNLTS KLVEPALAPEPLV+EPLV L D 
Sbjct: 301 NGSSLRVETTNFKDDLTVQGVTTEPNIIALDSNLTSAKLVEPALAPEPLVLEPLVFLDDT 360

Query: 411 LPPVECGVSIGHGLDELTPKHVEANGVFSSPTDLLSLDNMVSSSNTIATSIEALDESYVT 470
           LPPVECGV IGHGLDE+TPKHVE NGVFSSPTDLL +DN+VSSSNTI T +EA++ES VT
Sbjct: 361 LPPVECGVLIGHGLDEVTPKHVEVNGVFSSPTDLLLIDNVVSSSNTIETPVEAVEESCVT 420

Query: 471 RNEEYETECRETEKAEILPTKATSEAGSEAQPVSSDAVQMAPNILEMGDAYKLALGARGG 530
           R+EEYE E R TEKAEILPTKATSEAGSE QPVSSD+ QMAP +LE+GDAYKLA+GARGG
Sbjct: 421 RSEEYEKESRGTEKAEILPTKATSEAGSEVQPVSSDSAQMAPIMLELGDAYKLAVGARGG 480

Query: 531 RQLSGKLSEQWLERDSSRVSEDLKLLLSQLSFNRMNDQSRDMSPRLSINGDESRNFDVSS 590
           RQLSGKL EQW+ ++SS+VSEDLKLLLSQLSFNRMNDQSRDMSPRLS+NGDE RNFD SS
Sbjct: 481 RQLSGKLLEQWIGKESSKVSEDLKLLLSQLSFNRMNDQSRDMSPRLSVNGDEVRNFDYSS 540

Query: 591 AVGMQMLQKRISLERNESGLESLDGSIVTEIEGENVVDRLKRQIEYDKRIMNSLYKELDE 650
           AVGMQMLQ+RISLERNESGLESLDGSI++EI+GENV DRLKRQ+EYDK++M+SLYKEL+E
Sbjct: 541 AVGMQMLQRRISLERNESGLESLDGSIISEIDGENVADRLKRQVEYDKKVMSSLYKELEE 600

Query: 651 ERNASAIATNQAMAMITRLQEEKATLHMEALQCLRMMEEQNEYDDDALQKANDLISEKDK 710
           ERNASAIATNQAMAMITRLQEEKA LHMEALQCLRMMEEQ+EYDDDALQKANDLI+EKDK
Sbjct: 601 ERNASAIATNQAMAMITRLQEEKANLHMEALQCLRMMEEQSEYDDDALQKANDLITEKDK 660

Query: 711 EIQDLEAELEFYRINFPNAYTIDNLVETSNVKERDIRVVHLESNQIGTIGYGNSVAGEPD 770
           EIQDLEAELEFYRINFPNAYTIDNLVETS VKERDI VVHLESNQ GTIG GN +AG+PD
Sbjct: 661 EIQDLEAELEFYRINFPNAYTIDNLVETS-VKERDIGVVHLESNQFGTIGNGNLIAGKPD 720

Query: 771 IHEKVGSEGSTYNNLLLEFEDEKLDIMQCLKMLENRLHLFSNNGDKIDLSNGEYFG---- 830
           +HEKVGSEGSTYNNLLLEFEDEKL+IMQ LK LEN LHLFSN+G K+DLSNGEY G    
Sbjct: 721 LHEKVGSEGSTYNNLLLEFEDEKLNIMQRLKKLENMLHLFSNDGIKMDLSNGEYIGNERS 780

Query: 831 ------DFDSDNRKLEDGKDHACLPGKDLHDDDDRLPSLANPSLSKESSELDCSDRNSLL 871
                 D D D+RKLED + HACLPG+D H +DD LPSL NPS  KES+ELDCSDRNSLL
Sbjct: 781 FSSGTNDLDLDDRKLEDREHHACLPGEDAHIEDDHLPSLTNPSFDKESNELDCSDRNSLL 840

BLAST of Spg003486 vs. ExPASy TrEMBL
Match: A0A1S4E288 (myosin-binding protein 1-like OS=Cucumis melo OX=3656 GN=LOC103496267 PE=4 SV=1)

HSP 1 Score: 1231.5 bits (3185), Expect = 0.0e+00
Identity = 659/906 (72.74%), Postives = 724/906 (79.91%), Query Frame = 0

Query: 51  MGTSSVEAHTERDLFTSLLSAVFEWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRL 110
           MGTSSVEA TER LFT+L+SAVFEWLLICMLF DSIFSFFITKCAHFWKL  PCLLCSRL
Sbjct: 1   MGTSSVEARTERSLFTALVSAVFEWLLICMLFADSIFSFFITKCAHFWKLCTPCLLCSRL 60

Query: 111 DHVFSSEKRGYLWKLICGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKSNSET 170
           DH+F SEKRGY+W LIC KH+ E+S LVLCHAHNKLVNVHEMCENCLFSFAT NKSNSET
Sbjct: 61  DHIFGSEKRGYIWNLICSKHKLELSSLVLCHAHNKLVNVHEMCENCLFSFATFNKSNSET 120

Query: 171 YRLLVGKLGEDPRHVIDRHPLLGDQKHDSLSEKHCSCCKEPYVSRAFVQKLIQTRSNGLE 230
           YRLLVGKLG+DP   IDR PLLGDQK+D+LS+K CSCCKE YV R F Q LIQTRS+GLE
Sbjct: 121 YRLLVGKLGKDPYPRIDRDPLLGDQKYDTLSQKCCSCCKELYVPRGFAQSLIQTRSSGLE 180

Query: 231 TKDLDVPLSSSVVHCKEDFQEASSNALPQVQYRELKITS--------------------- 290
            +DLDVPL+SS VH   D QE S+N  P VQYRELKITS                     
Sbjct: 181 AEDLDVPLASSAVHYDGDSQECSNNPRPHVQYRELKITSDTESEGNGSILGVERANSLKD 240

Query: 291 -------------------------------------------------------DTESD 350
                                                                  DTESD
Sbjct: 241 DLTIQDVNMEPNFISLASNLTSTKLIEPALAPEPLVLEPLATTPYVQNRELKITPDTESD 300

Query: 351 GYGSTLGVETANSKDDFTVQGVHMEPNLISLDSNLTSTKLVEPALAPEPLVIEPLVSLGD 410
           G GSTL VET NSKDD TV GV+ EPN+ISLDSNLTS KLVEPALAPEPLV+EPLVSL D
Sbjct: 301 GNGSTLRVETTNSKDDLTVLGVNTEPNIISLDSNLTSAKLVEPALAPEPLVLEPLVSLDD 360

Query: 411 ALPPVECGVSIGHGLDELTPKHVEANGVFSSPTDLLSLDNMVSSSNTIATSIEALDESYV 470
           ALPP ECGV IGHGLDE+TPK VE NG FSSP+DLL +DN+VSSSNT  T +EA++ES V
Sbjct: 361 ALPPAECGVLIGHGLDEVTPKLVEVNGFFSSPSDLLPIDNVVSSSNTKETPVEAVEESCV 420

Query: 471 TRNEEYETECRETEKAEILPTKATSEAGSEAQPVSSDAVQMAPNILEMGDAYKLALGARG 530
            R+EEYE E R TEKAEILPTKATSEAGSE QPVSSD+ QM PN+LE+GDAYKLA+GARG
Sbjct: 421 ERSEEYEKESRGTEKAEILPTKATSEAGSEVQPVSSDSAQMVPNMLELGDAYKLAVGARG 480

Query: 531 GRQLSGKLSEQWLERDSSRVSEDLKLLLSQLSFNRMNDQSRDMSPRLSINGDESRNFDVS 590
           GRQLSGKL EQW+ ++SS+VSEDLKLLL+QLSFNR+NDQSR+MSPRLS+NGDE RNFD  
Sbjct: 481 GRQLSGKLLEQWIGKESSKVSEDLKLLLTQLSFNRLNDQSREMSPRLSVNGDEVRNFDYL 540

Query: 591 SAVGMQMLQKRISLERNESGLESLDGSIVTEIEGENVVDRLKRQIEYDKRIMNSLYKELD 650
           SAVGMQMLQKRISLERNESG+ESLDGSI++EI+GEN+ DRLKRQIEYDK++M+SLYKEL+
Sbjct: 541 SAVGMQMLQKRISLERNESGVESLDGSIISEIDGENMADRLKRQIEYDKKVMSSLYKELE 600

Query: 651 EERNASAIATNQAMAMITRLQEEKATLHMEALQCLRMMEEQNEYDDDALQKANDLISEKD 710
           EERNASAIA NQAMAMITRLQEEKA LHMEAL CLRMMEEQ+EYDDDALQKANDLI+EKD
Sbjct: 601 EERNASAIAANQAMAMITRLQEEKANLHMEALHCLRMMEEQSEYDDDALQKANDLITEKD 660

Query: 711 KEIQDLEAELEFYRINFPNAYTIDNLVETSNVKERDIRVVHLESNQIGTIGYGNSVAGEP 770
           KEIQDLEAELEFYRINFPNAYTIDNLVETS VKERDIRVVHLESNQI  IG  N V G+P
Sbjct: 661 KEIQDLEAELEFYRINFPNAYTIDNLVETS-VKERDIRVVHLESNQIEPIGNRNLVTGKP 720

Query: 771 DIHEKVGSEGSTYNNLLLEFEDEKLDIMQCLKMLENRLHLFSNNGDKIDLSNGEYFG--- 830
           D+HE+VGSEGSTYNNLLLEFEDEKL+I Q LK LEN LHLFSN+G K+DLSNG+YFG   
Sbjct: 721 DLHEEVGSEGSTYNNLLLEFEDEKLNIKQHLKKLENMLHLFSNDGVKMDLSNGKYFGNKR 780

Query: 831 -------DFDSDNRKLEDGKDHACLPGKDLHDDDDRLPSLANPSLSKESSELDCSDRNSL 871
                  D D D+RKLED + HACLPG+D H +DD LPSL N S  KESSELDCSD+NS 
Sbjct: 781 SFSSGTNDLDLDDRKLEDREHHACLPGEDAHIEDDHLPSLTNSSFDKESSELDCSDKNSP 840

BLAST of Spg003486 vs. ExPASy TrEMBL
Match: A0A5D3BFT5 (Myosin-binding protein 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001250 PE=4 SV=1)

HSP 1 Score: 1227.2 bits (3174), Expect = 0.0e+00
Identity = 658/906 (72.63%), Postives = 722/906 (79.69%), Query Frame = 0

Query: 51  MGTSSVEAHTERDLFTSLLSAVFEWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRL 110
           MGTSSVEA TER LFT+L+SAVFEWLLICMLF DSIFSFFITKCAHFWKL  PCLLCSRL
Sbjct: 1   MGTSSVEARTERSLFTALVSAVFEWLLICMLFADSIFSFFITKCAHFWKLCTPCLLCSRL 60

Query: 111 DHVFSSEKRGYLWKLICGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKSNSET 170
           DH+F SEKRGY+W LIC KH+ E+S LVLCHAHNKLVNVHEMCENCLFSFAT NKSNSET
Sbjct: 61  DHIFGSEKRGYIWNLICSKHKLELSSLVLCHAHNKLVNVHEMCENCLFSFATFNKSNSET 120

Query: 171 YRLLVGKLGEDPRHVIDRHPLLGDQKHDSLSEKHCSCCKEPYVSRAFVQKLIQTRSNGLE 230
           YRLLVGKLG+DP   IDR PLLGDQK+D+LS+K CSCCKE YV R F Q LIQTRS+GLE
Sbjct: 121 YRLLVGKLGKDPYPRIDRDPLLGDQKYDTLSQKCCSCCKELYVPRGFAQSLIQTRSSGLE 180

Query: 231 TKDLDVPLSSSVVHCKEDFQEASSNALPQVQYRELKITS--------------------- 290
            +DLDVPL+SS VH   D QE SSN  P VQYRELKITS                     
Sbjct: 181 AEDLDVPLASSAVHYDGDSQECSSNPRPHVQYRELKITSDTESEGNGSILGVERANSLKD 240

Query: 291 -------------------------------------------------------DTESD 350
                                                                  DTESD
Sbjct: 241 DLTIQDVNMEPNFISLASNLTSTKLIEPALAPEPLVLEPLATTPYVQNRELKITPDTESD 300

Query: 351 GYGSTLGVETANSKDDFTVQGVHMEPNLISLDSNLTSTKLVEPALAPEPLVIEPLVSLGD 410
           G GSTL VET NSKDD TV GV+ EPN+ISLDSNLTS KLVEPALAPEPLV+EPLVSL D
Sbjct: 301 GNGSTLRVETTNSKDDLTVLGVNTEPNIISLDSNLTSAKLVEPALAPEPLVLEPLVSLDD 360

Query: 411 ALPPVECGVSIGHGLDELTPKHVEANGVFSSPTDLLSLDNMVSSSNTIATSIEALDESYV 470
           ALPP ECGV IGHGLDE+TPK VE NG FSSP+DLL +DN+VSSSNT  T +EA++ES V
Sbjct: 361 ALPPAECGVLIGHGLDEVTPKLVEVNGFFSSPSDLLPIDNVVSSSNTKETPVEAVEESCV 420

Query: 471 TRNEEYETECRETEKAEILPTKATSEAGSEAQPVSSDAVQMAPNILEMGDAYKLALGARG 530
            R+EEYE E R TEKAEILPTKATSEAGSE QPVSSD+ QM PN+LE+GDAYKLA+GARG
Sbjct: 421 ERSEEYEKESRGTEKAEILPTKATSEAGSEVQPVSSDSAQMVPNMLELGDAYKLAVGARG 480

Query: 531 GRQLSGKLSEQWLERDSSRVSEDLKLLLSQLSFNRMNDQSRDMSPRLSINGDESRNFDVS 590
           GRQLSGKL EQW+ ++SS+VSEDLKLLL+QLSFNR+NDQSR+MSPRLS+NGDE RNFD  
Sbjct: 481 GRQLSGKLLEQWIGKESSKVSEDLKLLLTQLSFNRLNDQSREMSPRLSVNGDEVRNFDYL 540

Query: 591 SAVGMQMLQKRISLERNESGLESLDGSIVTEIEGENVVDRLKRQIEYDKRIMNSLYKELD 650
           SAVGMQMLQKR SLERNESG+ESLDGSI++EI+GEN+ DRLKRQIEYDK++M+SLYKEL+
Sbjct: 541 SAVGMQMLQKRCSLERNESGVESLDGSIISEIDGENMADRLKRQIEYDKKVMSSLYKELE 600

Query: 651 EERNASAIATNQAMAMITRLQEEKATLHMEALQCLRMMEEQNEYDDDALQKANDLISEKD 710
           EERNASAIA NQAMAMITRLQEEKA LHMEAL CLRMMEEQ+EYDDDALQKANDLI+EKD
Sbjct: 601 EERNASAIAANQAMAMITRLQEEKANLHMEALHCLRMMEEQSEYDDDALQKANDLITEKD 660

Query: 711 KEIQDLEAELEFYRINFPNAYTIDNLVETSNVKERDIRVVHLESNQIGTIGYGNSVAGEP 770
           KEIQDLEAELEFYRINFPNAYTIDNLVETS VKERDIRVVHLESNQI  IG  N V G+P
Sbjct: 661 KEIQDLEAELEFYRINFPNAYTIDNLVETS-VKERDIRVVHLESNQIEPIGNRNLVTGKP 720

Query: 771 DIHEKVGSEGSTYNNLLLEFEDEKLDIMQCLKMLENRLHLFSNNGDKIDLSNGEYFG--- 830
           D+HE+VGSEGSTYN LLLEFEDEKL+I Q LK LEN LHLFSN+G K+DLSNG+YFG   
Sbjct: 721 DLHEEVGSEGSTYNILLLEFEDEKLNIKQHLKKLENMLHLFSNDGVKMDLSNGKYFGNKR 780

Query: 831 -------DFDSDNRKLEDGKDHACLPGKDLHDDDDRLPSLANPSLSKESSELDCSDRNSL 871
                  D D D+RKLED + HACLPG+D H +DD LPSL N S  KESSELDCSD+NS 
Sbjct: 781 SFSSGTNDLDLDDRKLEDREHHACLPGEDAHIEDDHLPSLTNSSFDKESSELDCSDKNSP 840

BLAST of Spg003486 vs. ExPASy TrEMBL
Match: A0A6J1G417 (myosin-binding protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111450545 PE=4 SV=1)

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 621/860 (72.21%), Postives = 686/860 (79.77%), Query Frame = 0

Query: 51  MGTSSVEAHTERDLFTSLLSAVFEWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRL 110
           MGT SVEA T   L T LLSAV E LLICMLF+ SIFSFFITKCA  WKL  PCLLCSRL
Sbjct: 1   MGTLSVEACTGGSLRTYLLSAVSECLLICMLFLHSIFSFFITKCARLWKLRIPCLLCSRL 60

Query: 111 DHVFSSEKRGYLWKLICGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKSNSET 170
           DHVF SEK+GYLW LICGKH+ E+S LVLCHAHNKLVNVHEMCENCLFSFATINK NSET
Sbjct: 61  DHVFGSEKKGYLWTLICGKHKLEVSSLVLCHAHNKLVNVHEMCENCLFSFATINKLNSET 120

Query: 171 YRLLVGKLGEDPRHVIDRHPLLGDQKHDSLSEK--------------------------- 230
           YRLLVGKLGEDP   ID  P LGDQKH S  E+                           
Sbjct: 121 YRLLVGKLGEDPHFGIDSDPSLGDQKHCSCCEESYIPRGFVQTLIQTRSSGLKVEDLNVP 180

Query: 231 ------------HCSCCKEPYVSRAFVQKLIQTRSNGLETKDLDVPLSSSVVHCKEDFQE 290
                         SCC+E YV R FVQ  IQTRS+GL+ +DLDVPLSSS+V CK D Q 
Sbjct: 181 LSSSIVPCKVDVQGSCCEESYVPRGFVQTSIQTRSSGLKAEDLDVPLSSSIVPCKVDIQG 240

Query: 291 ASSNALPQVQYRELKITSDTESDGYGSTLGVETANSKDDFTVQGVHMEPNLISLDSNLTS 350
             SN LP VQY+EL ITSDTESDG G TL VETANSKDD  +Q  +ME N  SL SNLTS
Sbjct: 241 PPSNPLPHVQYKELNITSDTESDGNGGTLVVETANSKDDLALQDDNMEANFSSLASNLTS 300

Query: 351 TKLVEPALAPEPLVIEPLVSLGD-ALPPVECGVSIGHGLDELTPKHVEANGVFSSPTDLL 410
           T LVEPALAPEPLV+     LGD ALP VE GVSIGHGLDE TPKHVEAN  FSSP DLL
Sbjct: 301 TTLVEPALAPEPLVL-----LGDAALPRVERGVSIGHGLDESTPKHVEANDGFSSPNDLL 360

Query: 411 SLDNMVSSSNTIATSIEALDESYVTRNEEYETECRETEKAEILPTKATSEAGSEAQPVSS 470
           SLDNMV SSNTI TS+EA++ESYVTR+EE+ET+ R TEKAEI PTKATSE  +E QPVSS
Sbjct: 361 SLDNMVPSSNTIVTSVEAVEESYVTRSEEHETKSRGTEKAEISPTKATSEEDTETQPVSS 420

Query: 471 DAVQMAPNILEMGDAYKLALGARGGRQLSGKLSEQWLERDSSRVSEDLKLLLSQLSFNRM 530
           D  QMAPN LE+GDAYK+A+GAR GRQLSGKLSEQW+E+DSS+VS+DLKLL++QLSFNR 
Sbjct: 421 DTAQMAPNTLELGDAYKIAVGARAGRQLSGKLSEQWIEKDSSKVSDDLKLLMTQLSFNRT 480

Query: 531 NDQSRDMSPRLSINGDESRNFDVSSAVGMQMLQKRISLERNESGLESLDGSIVTEIEGEN 590
            DQSR+MSPRLSINGD     DVS+ VGMQ+ QKRISLERNES L+SLDGSIV+EIEGEN
Sbjct: 481 TDQSREMSPRLSINGD-----DVSNIVGMQIPQKRISLERNESSLDSLDGSIVSEIEGEN 540

Query: 591 VVDRLKRQIEYDKRIMNSLYKELDEERNASAIATNQAMAMITRLQEEKATLHMEALQCLR 650
           VVDRLKRQ+EYDK++M SLYKEL+EERNASAIA NQAMAMITRLQEEKA LHMEALQCLR
Sbjct: 541 VVDRLKRQVEYDKKLMISLYKELEEERNASAIAANQAMAMITRLQEEKANLHMEALQCLR 600

Query: 651 MMEEQNEYDDDALQKANDLISEKDKEIQDLEAELEFYRINFPNAYTIDNLVETSNVKERD 710
           MMEEQ+EYDDDALQKANDLI+EKDKEIQDLEAELEF+RINFPNAYTIDNL+E S VKERD
Sbjct: 601 MMEEQSEYDDDALQKANDLITEKDKEIQDLEAELEFFRINFPNAYTIDNLIEMS-VKERD 660

Query: 711 IRVVHLESNQIGTIGYGNSVAGEPDIHEKVGSEGSTYNNLLLEFEDEKLDIMQCLKMLEN 770
           I VVHLESNQ+GTIGYGN V G+PDIHEKVGSEG T+NNLL EFEDEK++I+QCL+ LEN
Sbjct: 661 IGVVHLESNQLGTIGYGNLVVGKPDIHEKVGSEGGTFNNLLSEFEDEKINILQCLQKLEN 720

Query: 771 RLHLFSNNGDKIDLSNGEYFGDFDSDNRKLEDGKDHACLPGKDLHDDDDRLPSLANPSLS 830
            +HLFS NG K+DLSNGEY G   +D+ KLE+G+DHA     D +D+DD LPSLANP   
Sbjct: 721 MIHLFSINGVKMDLSNGEYLG---TDDLKLENGEDHA-KDDDDDNDNDDCLPSLANPPFD 780

Query: 831 KESSELDCSDRNSLLATGMADFASLRNEMSNLNKRMEVLETDKNFLEHTINSLKKGEEGL 871
           KES+ELD SDRNS   T   DFA LR E+S LNKRMEVLE DKN LEHTINSL +GEEGL
Sbjct: 781 KESNELDRSDRNSSSNTEREDFAFLRIEVSKLNKRMEVLEADKNILEHTINSLIRGEEGL 840

BLAST of Spg003486 vs. ExPASy TrEMBL
Match: A0A6J1KBZ4 (myosin-binding protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493560 PE=4 SV=1)

HSP 1 Score: 1110.1 bits (2870), Expect = 0.0e+00
Identity = 622/906 (68.65%), Postives = 691/906 (76.27%), Query Frame = 0

Query: 51  MGTSSVEAHTERDLFTSLLSAVFEWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRL 110
           MGT SVEA T   L T LLSAV E LLICMLF+ SIFSFFITKCA  WKL  PCLLCSRL
Sbjct: 1   MGTLSVEACTGGSLRTYLLSAVSECLLICMLFLHSIFSFFITKCARLWKLRTPCLLCSRL 60

Query: 111 DHVFSSEKRGYLWKLICGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKSNSET 170
           DHVF S+K+GYLW LICGKH+ E+S LVLCHAHNKLVNVH++CENCLFSFATINK NSET
Sbjct: 61  DHVFGSQKKGYLWTLICGKHKLEVSSLVLCHAHNKLVNVHDVCENCLFSFATINKLNSET 120

Query: 171 YRLLVGKLGEDPRHVIDRHPLLGDQKHDSLSEKH-------------------------- 230
           YRLLVGKLGEDP   ID  PLLGDQKH S  E+                           
Sbjct: 121 YRLLVGKLGEDPHFGIDSDPLLGDQKHCSCCEESYVPRGFVQTLIQTRSSGLKAEDLNVP 180

Query: 231 -----------------------------------------------------C------ 290
                                                                C      
Sbjct: 181 LSSSIVPCKVDTQGSCCEESYVPRGFVQTLIQTRSSELKAEDLDVPLSSSIVPCKVDIQE 240

Query: 291 SCCKEPYVSRAFVQKLIQTRSNGLETKDLDVPLSSSVVHCKEDFQEASSNALPQVQYREL 350
           SCC+EP V R FVQ LIQTRS+ L+ +DLDVPLSSS+V CK D Q   SN LP VQY+EL
Sbjct: 241 SCCEEPCVPRGFVQTLIQTRSSELKAEDLDVPLSSSIVPCKVDIQGPPSNPLPHVQYKEL 300

Query: 351 KITSDTESDGYGSTLGVETANSKDDFTVQGVHMEPNLISLDSNLTSTKLVEPALAPEPLV 410
            ITSDTESDG G TL VETANSKDDF +Q  +ME N ISL SN+T T L EPALAPEP V
Sbjct: 301 NITSDTESDGNGGTLVVETANSKDDFALQDDNMEVNFISLASNITLTTLDEPALAPEPSV 360

Query: 411 IEPLVSLG-DALPPVECGVSIGHGLDELTPKHVEANGVFSSPTDLLSLDNMVSSSNTIAT 470
           +     LG DALP VE GVSIGHGLDELTPKHVEAN  FSSP DLLSLDNMV SSNTI T
Sbjct: 361 V-----LGDDALPRVERGVSIGHGLDELTPKHVEANDGFSSPNDLLSLDNMVPSSNTIVT 420

Query: 471 SIEALDESYVTRNEEYETECRETEKAEILPTKATSEAGSEAQPVSSDAVQMAPNILEMGD 530
           S+EA++ESYVTR+EE+ET+ R TEKAEI PTKATSE  +E QPVSSDA QMAPN LE+GD
Sbjct: 421 SVEAVEESYVTRSEEHETKSRGTEKAEISPTKATSEEDTETQPVSSDAAQMAPNTLELGD 480

Query: 531 AYKLALGARGGRQLSGKLSEQWLERDSSRVSEDLKLLLSQLSFNRMNDQSRDMSPRLSIN 590
           AYK+A+GAR GRQLSGKLSEQW+E+DSS+VS+DLKLL++QLSFNR NDQSR+MSPRLSIN
Sbjct: 481 AYKIAVGARAGRQLSGKLSEQWIEKDSSKVSDDLKLLMTQLSFNRTNDQSREMSPRLSIN 540

Query: 591 GDESRNFDVSSAVGMQMLQKRISLERNESGLESLDGSIVTEIEGENVVDRLKRQIEYDKR 650
           GD     DVS+ +GMQ+ QKRI+LERNES L+SLDGSIV+EIEGENVVDRLKRQ+EYDK+
Sbjct: 541 GD-----DVSNVIGMQIPQKRITLERNESSLDSLDGSIVSEIEGENVVDRLKRQVEYDKK 600

Query: 651 IMNSLYKELDEERNASAIATNQAMAMITRLQEEKATLHMEALQCLRMMEEQNEYDDDALQ 710
           +M SLYKEL+EERNASAIA NQAMAMITRLQEEKA LHMEALQCLRMMEEQ+EYDDDALQ
Sbjct: 601 LMISLYKELEEERNASAIAANQAMAMITRLQEEKANLHMEALQCLRMMEEQSEYDDDALQ 660

Query: 711 KANDLISEKDKEIQDLEAELEFYRINFPNAYTIDNLVETSNVKERDIRVVHLESNQIGTI 770
           KANDLI+EKDKEIQDLEAELEF+RINFPNAYTIDNL+E S VKERDI VVHLESNQIGTI
Sbjct: 661 KANDLITEKDKEIQDLEAELEFFRINFPNAYTIDNLIEMS-VKERDIGVVHLESNQIGTI 720

Query: 771 GYGNSVAGEPDIHEKVGSEGSTYNNLLLEFEDEKLDIMQCLKMLENRLHLFSNNGDKIDL 830
           GYGN V G+PDIHEKVGSEG T+NNLL EFEDEK++I+QCLK LEN +HLFS NG K+DL
Sbjct: 721 GYGNLVVGKPDIHEKVGSEGGTFNNLLSEFEDEKINILQCLKKLENMIHLFSINGVKMDL 780

Query: 831 SNGEYFGDFDSDNRKLEDGKDHACLPGKDLHDDDDRLPSLANPSLSKESSELDCSDRNSL 871
           SNGEY G   +D+ KLE+G+DHA     D +D+DD LPSLANP   KES+ELD SDRNS 
Sbjct: 781 SNGEYLG---TDDLKLENGEDHAKDDDND-NDNDDCLPSLANPPFDKESNELDRSDRNSS 840

BLAST of Spg003486 vs. TAIR 10
Match: AT1G08800.1 (Protein of unknown function, DUF593 )

HSP 1 Score: 270.8 bits (691), Expect = 3.9e-72
Identity = 331/1213 (27.29%), Postives = 460/1213 (37.92%), Query Frame = 0

Query: 65   FTSLLSAVF-EWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRLDHVFSSEK--RGY 124
            FT  L+  F EWLL+ MLFV+SIFS+ I + A + +L +PCL+CS LDH+    K  +  
Sbjct: 6    FTRALALAFNEWLLMFMLFVNSIFSYVIARFADYSELQSPCLMCSNLDHILRRTKDLKKT 65

Query: 125  LWKLICGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKSNSETYRLLVGKLGED 184
             W +IC KH+ EIS LV CHAH KLV+V  MCE CLFSFAT NKSN+ETYRLLVGKLGED
Sbjct: 66   HWDIICSKHKSEISSLVYCHAHGKLVDVRGMCETCLFSFATTNKSNAETYRLLVGKLGED 125

Query: 185  PR--HVIDRHPLLGDQKHDSLSE-KHCSCCKEPY---------VSRAFVQKL-----IQT 244
                   DR       K+ + S+   C+CC + +           R  + K+     I+T
Sbjct: 126  SHFGSKSDR------SKYPNCSKLTDCTCCNQLWTPQTAATQVAEREILPKIGLLGKIRT 185

Query: 245  RSNGLETKDL------DVPLSSSVVHCKEDFQ---------------------------- 304
                   K +      DV  +   +H   + +                            
Sbjct: 186  GKQSAPKKSVSFNHLPDVGYTELKIHSDTESEAVFSDTEPKQESSLNHLPPVGYNEPKIG 245

Query: 305  ---------------EASSNALPQVQYRELKITSDTESDGYGS----------------- 364
                             S N LP V Y ELKI SDTES+   S                 
Sbjct: 246  LVGDVRTGKPSTPKKSVSFNHLPDVGYTELKIHSDTESEAVFSEDECVVLKDEDHKYQIV 305

Query: 365  --------TLGVETANSK---DDF------------------------------------ 424
                    TL  + A  K    DF                                    
Sbjct: 306  DLQTHPIITLPYDLATDKLLNFDFPLEPFVTRNDREEVQLQETNWRTYSSFPVLIPVNDV 365

Query: 425  --TVQGVHMEPNLISLD------------------------------------------- 484
              T + V  E  + SLD                                           
Sbjct: 366  PETSEKVFKEEEINSLDNLFLTSRAMKHFAAAKVKEEPIRLQDISSTPDVKENPANASLM 425

Query: 485  --------SNLTSTK----------------------LVEPALAPEPL------------ 544
                    S++T+T                        V P     P             
Sbjct: 426  EETELICLSDVTATSGAMEHSEVILKEREELIHLQDISVTPDFKENPANASLLEETELIC 485

Query: 545  ---VIEPL-------VSLGDALPPV----------------------------------E 604
               V  PL       V L D + P+                                   
Sbjct: 486  LNDVTSPLRAVEHSAVLLKDKVEPIRLQDGGSLTPDFMENSANASILEETELICVNDVTS 545

Query: 605  CGVSIGHGL--------------DELTPKHVE---------------------------- 664
               ++GH                  LTP   E                            
Sbjct: 546  TSRTMGHSSVVLKENEEPIRFQDSSLTPDFKENPASTFLVEETELICLNDVTSPSRAMEH 605

Query: 665  ---------------------------ANGVFSSPTDL---------------------- 724
                                       AN      T+L                      
Sbjct: 606  STVFIEEKEELVRHQNITLTQDFMENPANSSLREETELICLNDVTSTSEVAETPEDVLEG 665

Query: 725  --------LSLDNMVSSSNTIATSIE-------------ALDESYVT------------- 784
                    +SLD +  S  T  TS+E             +L+  Y+              
Sbjct: 666  IELMSIHDISLDEVSESVTTNQTSVEISKERDTDQADITSLESEYIVVPSPNSMPENSTD 725

Query: 785  -----RNEEYETECRETEKAEILPTKATS--EAGSEAQ-------PVSSDAVQMAPNILE 844
                 + E  ET  R +  +E+ P   TS  EA  E++        V+++  Q +  +L+
Sbjct: 726  NCVSDKKEMKETSLRISSLSEMAPRDVTSHTEAALESESSSFNSMSVAAETNQYSGELLD 785

Query: 845  MGDAYKLALGARGGRQLSGKLS-EQWLERDSSRVSEDLKLLLSQLSFNRMND--QSRDMS 870
            + DAY + +G  G    +G+   E W+++D+SRVSEDLK LL+Q+S +R  +    RD+S
Sbjct: 786  LADAYNIVVGNEGHYDSNGRQQIENWMKKDTSRVSEDLKALLTQISASRGIEFLSPRDVS 845

BLAST of Spg003486 vs. TAIR 10
Match: AT1G08800.2 (Protein of unknown function, DUF593 )

HSP 1 Score: 270.8 bits (691), Expect = 3.9e-72
Identity = 331/1213 (27.29%), Postives = 460/1213 (37.92%), Query Frame = 0

Query: 65   FTSLLSAVF-EWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRLDHVFSSEK--RGY 124
            FT  L+  F EWLL+ MLFV+SIFS+ I + A + +L +PCL+CS LDH+    K  +  
Sbjct: 6    FTRALALAFNEWLLMFMLFVNSIFSYVIARFADYSELQSPCLMCSNLDHILRRTKDLKKT 65

Query: 125  LWKLICGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKSNSETYRLLVGKLGED 184
             W +IC KH+ EIS LV CHAH KLV+V  MCE CLFSFAT NKSN+ETYRLLVGKLGED
Sbjct: 66   HWDIICSKHKSEISSLVYCHAHGKLVDVRGMCETCLFSFATTNKSNAETYRLLVGKLGED 125

Query: 185  PR--HVIDRHPLLGDQKHDSLSE-KHCSCCKEPY---------VSRAFVQKL-----IQT 244
                   DR       K+ + S+   C+CC + +           R  + K+     I+T
Sbjct: 126  SHFGSKSDR------SKYPNCSKLTDCTCCNQLWTPQTAATQVAEREILPKIGLLGKIRT 185

Query: 245  RSNGLETKDL------DVPLSSSVVHCKEDFQ---------------------------- 304
                   K +      DV  +   +H   + +                            
Sbjct: 186  GKQSAPKKSVSFNHLPDVGYTELKIHSDTESEAVFSDTEPKQESSLNHLPPVGYNEPKIG 245

Query: 305  ---------------EASSNALPQVQYRELKITSDTESDGYGS----------------- 364
                             S N LP V Y ELKI SDTES+   S                 
Sbjct: 246  LVGDVRTGKPSTPKKSVSFNHLPDVGYTELKIHSDTESEAVFSEDECVVLKDEDHKYQIV 305

Query: 365  --------TLGVETANSK---DDF------------------------------------ 424
                    TL  + A  K    DF                                    
Sbjct: 306  DLQTHPIITLPYDLATDKLLNFDFPLEPFVTRNDREEVQLQETNWRTYSSFPVLIPVNDV 365

Query: 425  --TVQGVHMEPNLISLD------------------------------------------- 484
              T + V  E  + SLD                                           
Sbjct: 366  PETSEKVFKEEEINSLDNLFLTSRAMKHFAAAKVKEEPIRLQDISSTPDVKENPANASLM 425

Query: 485  --------SNLTSTK----------------------LVEPALAPEPL------------ 544
                    S++T+T                        V P     P             
Sbjct: 426  EETELICLSDVTATSGAMEHSEVILKEREELIHLQDISVTPDFKENPANASLLEETELIC 485

Query: 545  ---VIEPL-------VSLGDALPPV----------------------------------E 604
               V  PL       V L D + P+                                   
Sbjct: 486  LNDVTSPLRAVEHSAVLLKDKVEPIRLQDGGSLTPDFMENSANASILEETELICVNDVTS 545

Query: 605  CGVSIGHGL--------------DELTPKHVE---------------------------- 664
               ++GH                  LTP   E                            
Sbjct: 546  TSRTMGHSSVVLKENEEPIRFQDSSLTPDFKENPASTFLVEETELICLNDVTSPSRAMEH 605

Query: 665  ---------------------------ANGVFSSPTDL---------------------- 724
                                       AN      T+L                      
Sbjct: 606  STVFIEEKEELVRHQNITLTQDFMENPANSSLREETELICLNDVTSTSEVAETPEDVLEG 665

Query: 725  --------LSLDNMVSSSNTIATSIE-------------ALDESYVT------------- 784
                    +SLD +  S  T  TS+E             +L+  Y+              
Sbjct: 666  IELMSIHDISLDEVSESVTTNQTSVEISKERDTDQADITSLESEYIVVPSPNSMPENSTD 725

Query: 785  -----RNEEYETECRETEKAEILPTKATS--EAGSEAQ-------PVSSDAVQMAPNILE 844
                 + E  ET  R +  +E+ P   TS  EA  E++        V+++  Q +  +L+
Sbjct: 726  NCVSDKKEMKETSLRISSLSEMAPRDVTSHTEAALESESSSFNSMSVAAETNQYSGELLD 785

Query: 845  MGDAYKLALGARGGRQLSGKLS-EQWLERDSSRVSEDLKLLLSQLSFNRMND--QSRDMS 870
            + DAY + +G  G    +G+   E W+++D+SRVSEDLK LL+Q+S +R  +    RD+S
Sbjct: 786  LADAYNIVVGNEGHYDSNGRQQIENWMKKDTSRVSEDLKALLTQISASRGIEFLSPRDVS 845

BLAST of Spg003486 vs. TAIR 10
Match: AT2G30690.1 (Protein of unknown function, DUF593 )

HSP 1 Score: 194.5 bits (493), Expect = 3.5e-49
Identity = 203/720 (28.19%), Postives = 315/720 (43.75%), Query Frame = 0

Query: 68  LLSAVFEWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRLDHVFSSEKRGYLWK-LI 127
           L  A  EW LI ++F+D++ S+ +   A + +L  PC LCS+L H          W+ L+
Sbjct: 19  LTYAACEWFLILLMFIDALLSYLLVWFARYCRLQMPCFLCSKLLHPLH-------WRFLL 78

Query: 128 CGKHRQEISCLVLCHAH-NKLVNVHEMCENCLFSFATINKSNSETYRLLVGKLGEDPRHV 187
           C  HR E+S  + C  H N L +   MC++CL SF  +   N +  RLL+GKLG D    
Sbjct: 79  CRNHRSEVSSYMSCQNHGNNLADCRGMCDDCLLSFTKMTGPNPDMNRLLLGKLGYD---- 138

Query: 188 IDRHPLLGDQKHDSLSEKHCSCCKEPYVSRAFVQKLIQTRSNGLETKDL-DVPLSSSVVH 247
                 L  + H +   + CSCC +P+ +R   Q+LI+  S G  +    ++P    +  
Sbjct: 139 ------LLSRSHFA-HPRSCSCCNKPWRTRHHTQRLIRLGSRGRNSSSKPNIPAPRHLTR 198

Query: 248 -----------------CKEDFQEASSNALPQVQYRELKITSDTESDGYGS--------- 307
                              E     S + +  V Y ELKI SD+ES+   S         
Sbjct: 199 RGSGGSLKKMRDHIATSGSEYVDVGSRDGMAHVGYTELKIHSDSESEFLFSDDDAFLHIT 258

Query: 308 TLGVETANSK-----------------------DDFTVQGVHMEPNLISLDSNL-----T 367
              VE +  +                        D   + +H+E N     S L      
Sbjct: 259 DFNVEPSEKRTHKSRRRKSFDDKKMSNHKQPVLQDNQYKKIHVEDNETVESSMLGYNLEN 318

Query: 368 STKLVEPALAPE-PLVIEPLVSLGDALP-----PVECGVSI---GHGLDELTPKHVEANG 427
            T+  +P  A E   V+  L+++ +A P     P +    +        E++     + G
Sbjct: 319 RTRQKQPVKAKEHDDVLSELITMSEARPFLLGSPRKYAAGVVTQNENEAEVSGSSSPSGG 378

Query: 428 VFSSPTDLLSLDNMVSSSNTIATSIEALD-----ESYVTRNEEYET--ECRETEKAEILP 487
            F SP    S +N  S    I    ++ D      S     EE+E   E +E++  ++  
Sbjct: 379 EFLSP----SAENGASREIRIQEHDDSSDFSQNITSSAMEIEEFEAAIEQKESDHMDVSG 438

Query: 488 TKATSEAGSEAQPVSSDAVQMAPNILEMGDAYKLALGARGGRQLS-GKLSEQWL----ER 547
           + A   +  E   V  D+  +  N   M D+ +         +++   ++E++     E 
Sbjct: 439 SVANEPSSDEENEVEGDSKPLISN--NMSDSLEQEQSGEEESEVNENNVAEEYFSNEEED 498

Query: 548 DSSRVSEDLKLLLSQLSF-NRMNDQSRDMSPRLSINGDESRN-FDVSSAVGMQMLQKRI- 607
           + +  +E L       SF    + +  D S   S+  D S N  DV +     M    + 
Sbjct: 499 EVNGHTEPLTSKSESGSFAEEQSSEDEDGSNIYSVAKDHSSNEEDVDNEESEPMTSNNVT 558

Query: 608 -------------------------SLERNESGLESLDG--------------------- 654
                                    ++ + E  LE  D                      
Sbjct: 559 GVVKEEHSAKEEHGDHEETEPLTSLNISKEEPSLEHSDKDSLKITETRNTSNGSPELKHS 618

BLAST of Spg003486 vs. TAIR 10
Match: AT1G18990.1 (Protein of unknown function, DUF593 )

HSP 1 Score: 150.2 bits (378), Expect = 7.6e-36
Identity = 176/724 (24.31%), Postives = 279/724 (38.54%), Query Frame = 0

Query: 51  MGTSSVEAHTERDLFT---SLLSAVFEWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLC 110
           M   S +   E +L T    L+ A+ EW+LI +LF+D   +FF  + A F+ L  PCLLC
Sbjct: 1   MSNRSFKNFIEEELGTFPQFLIYALLEWILIIILFIDGFLAFFSNQIAKFFDLKIPCLLC 60

Query: 111 SRLDHVFSSEKRGYLWK-LICGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKS 170
           +RLDHV  S    + +   IC  H++ +S L  CH H KL  +  MCE CL SFAT  ++
Sbjct: 61  TRLDHVLVSRNPDFYYNDSICDAHKKNVSSLAYCHVHKKLSEIKRMCEGCLLSFATEKET 120

Query: 171 NSETYRLLVGKLGEDPRHVID-------RHPLLGDQKHDSLSEKHCSCCKEPYVSRAFVQ 230
           + +TY+ L+G L +D   +ID         P+ G +K ++  +                 
Sbjct: 121 DVDTYKSLIGILHKDLELLIDDERELQLAFPVAGSKKDENFYQ----------------- 180

Query: 231 KLIQTRSNGLETKDLDVPLSSSVVHCKEDFQ-EASSNALPQVQYRELKITSDTESDGYGS 290
             ++ R+N                +  + FQ +   +   Q+    +K+ SD       S
Sbjct: 181 --VENRTN----------------NSNDRFQRQQRCSCCGQI----MKLKSDKPKSNNQS 240

Query: 291 TLGVETANSKDDFTVQGVHMEPNLISLD-SNLTSTKLVEPALAPEPLVIEPLVSLGDALP 350
             G  + + +  F         N  +LD SN+  T L E                 DAL 
Sbjct: 241 FFGAPSPSPRVSF---------NQRTLDLSNIKYTDLPED---------------DDAL- 300

Query: 351 PVECGVSIGHGLDELTPKHVEANGVFSSPTDLLSLDNMVSSSNTIATSIEALDESYVTRN 410
                                                     NT   S++A+D+      
Sbjct: 301 ------------------------------------------NTKGASLDAVDD------ 360

Query: 411 EEYETECRETEKAEILPTKATSEAGSEAQPVSSDAVQMAPNILEMGDAYKLALGARGGRQ 470
                                                  P+ ++ G+ +           
Sbjct: 361 -------------------------------------RTPSFVKGGNKF----------- 420

Query: 471 LSGKLSEQWLERDSSRVSEDLKLLLSQLSFNRMNDQSRDMSPRLSINGDESRNFDVSSAV 530
                                        F      S   SPR S+   +    D     
Sbjct: 421 -----------------------------FGIPLSDSAQNSPRWSVRSMKKSFVD----- 480

Query: 531 GMQMLQKRISLERNESGLESLDGSIVTEIEGENVVDRLKRQIEYDKRIMNSLYKELDEER 590
                         ++GLES        ++G++++  L RQ+  D++ +  LY ELDEER
Sbjct: 481 --------------QNGLES------EVLDGDSILQHLNRQVRLDRKSLMDLYMELDEER 501

Query: 591 NASAIATNQAMAMITRLQEEKATLHMEALQCLRMMEEQNEYDDDALQKANDLISEKDKEI 650
           +ASA+A N AMAMITRLQ EKA + MEALQ  RMM+EQ EYD +ALQ  N L+ ++++E+
Sbjct: 541 SASAVAANNAMAMITRLQAEKAAVQMEALQYQRMMDEQAEYDQEALQSMNGLLVKREEEM 501

Query: 651 QDLEAELEFYRINFPNAYTIDNLVETSNVKERDIRVVHLESNQIGTIGYGNSVAGEPDIH 710
           ++LEA +E YR+ +        L+     +  +   +  E+  +  +   +S   E    
Sbjct: 601 KELEAGIEVYRLRY-------GLLREERGEAEEF--LDEETKPVSDLPVCSSNHEEDLEQ 501

Query: 711 EKVGSEGSTYNNLLLEFEDEK-------------LDIMQCLKMLENRLHLFSNNGDKIDL 749
            K  +E S  NN ++  E+EK              +I + L  +E++  L     D +D+
Sbjct: 661 MKDSAEDSIGNNGVMIIEEEKENGSRKDMLVKEISEITERLNAIESKGELLQQISDVLDV 501

BLAST of Spg003486 vs. TAIR 10
Match: AT1G74830.1 (Protein of unknown function, DUF593 )

HSP 1 Score: 141.7 bits (356), Expect = 2.7e-33
Identity = 174/700 (24.86%), Postives = 266/700 (38.00%), Query Frame = 0

Query: 68  LLSAVFEWLLICMLFVDSIFSFFITKCAHFWKLHAPCLLCSRLDHVF-SSEKRGYLWKLI 127
           L+  V EW LI  LF+D + +F   + A F+ L+ PCLLC+R+DH+    + + Y  + I
Sbjct: 40  LIYTVLEWSLIVFLFIDGVIAFLSNQFAKFFDLNIPCLLCTRIDHILVPRDPQFYYNESI 99

Query: 128 CGKHRQEISCLVLCHAHNKLVNVHEMCENCLFSFATINKSNSETYRLLVGKLGEDPRHVI 187
           C  H++++S L  CH H KL  +  MCE CL SFAT   S+ +TY+ L+G L +D   +I
Sbjct: 100 CDSHKKKVSSLAYCHVHKKLSEIKHMCEGCLLSFATEKDSDCDTYKSLIGILHKDLELLI 159

Query: 188 DRHPLLGDQKHDSLSEKHCSCCKEPYVSRAFVQKLIQTRSNGLETKDLDVPLSSSVVHCK 247
           D      D++   L+ K                 L+QT  N ++ K  ++   S   HC 
Sbjct: 160 D------DERDLPLAFKK-------------DDNLVQTTKNLVDYKTNNIKNDSLKQHC- 219

Query: 248 EDFQEASSNALPQVQYRELKITSDTESDGYGSTLGVETANSKDDFTVQGVHMEPNLISLD 307
                +    L       LKI S+       S L                          
Sbjct: 220 -----SCCGEL-------LKIKSEKLPKNNNSFL-------------------------- 279

Query: 308 SNLTSTKLVEPALAPEPLVIEPLVSLGDALPPVECGVSIGHGLDELTPKHVEANGVFSSP 367
                      A AP P V                                         
Sbjct: 280 -----------APAPSPRV----------------------------------------- 339

Query: 368 TDLLSLDNMVSSSNTIATSIEALDESYVTRNEEYETECRETEKAEILPTKATSEAGSEAQ 427
                      S N ++               E E+E ++ +                  
Sbjct: 340 -----------SHNKLS---------------ENESEFKDMD------------------ 399

Query: 428 PVSSDAVQMAPNILEMGDAYKLALGARGGRQLSGKLSEQWLERDSSRVSEDLKLLLSQLS 487
                 V   P+ +            RGG +  G                   + LS  +
Sbjct: 400 ------VDRTPSFV------------RGGNKFFG-------------------IPLSDSA 459

Query: 488 FNRMNDQSRDMSPRLSINGDESRNFDVSSAVGMQMLQKRISLERNESGLESLDGSIVTEI 547
            N         SPR S+                    K+  L + E+  ++ D +     
Sbjct: 460 QN---------SPRWSVRS-----------------LKKSVLNKTENASDTTDPT----- 516

Query: 548 EGENVVDRLKRQIEYDKRIMNSLYKELDEERNASAIATNQAMAMITRLQEEKATLHMEAL 607
            GE+++++LK+++  DK+ +  LY ELDEER+ASA+A N+AMAMITRLQ EKA + MEAL
Sbjct: 520 -GESILNQLKKEVRLDKKSLIDLYMELDEERSASAVAANEAMAMITRLQAEKAAVQMEAL 516

Query: 608 QCLRMMEEQNEYDDDALQKANDLISEKDKEIQDLEAELEFYRINF--------------- 667
           Q  RMM+EQ EYD +ALQ  +  ++++++E+++LEAE E YR  +               
Sbjct: 580 QYQRMMDEQAEYDQEALQSMSSELAKREEEMKELEAEFEVYREKYGCLTDQEDAREEFHK 516

Query: 668 --PNAYTIDNLVETSNVKERDIRVVHLESNQIGTIGYGNSVAGEPDIHEKVGSEGSTYNN 727
              NA   D+  ET  V +  +   + + N       G S   E    E V S      +
Sbjct: 640 QNGNASAYDDCQETKPVSDLAVSSSNQQENGENIDQNGQSKRSEESTAENVVSADEEKGS 516

Query: 728 LLLE-FEDEKLDIMQCLKMLENRLHLFSNNGDKIDLSNGE 749
              E    E  +I + L  L++   L  +  D +D+S GE
Sbjct: 700 ESKEGIVKELSEITERLSTLQSNGDLLKHIADVLDVSEGE 516