Spg002942 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg002942
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold6: 3121808 .. 3123765 (+)
RNA-Seq Expression	Spg002942
Synteny	Spg002942

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGCTCCATTTTGCTTTAGTTTTCGTCTTTTCCATTGCCATTTTGAGCTCATGGACTATATCTGCTTCGACTTACTTCAATGTTCTTGATTATGGAGCTGCTGGAAATGGCAAAACCGATGATTCAGAAGTAAGAAATTTAAGCTATATATACATTTTGATGTTTTTGAAGTACTTAATTATGATTCTCCTTCTAATCTTATAAGTTTTCGTCTCTCTTTCTCTTTGTGATAAGGCTTTTTTGAGAGCTTGGAAGGATGTGTGCGGAGCAACGGAAGGCAGTCCAACATTGCATGTACCGCTTGCAAAAACTTACCTGTTGAAGCCTCTCAAATTCCAGGGCCCTTGCAAGTCGAAACAAATCATTTTCCATGTAATTTATCTTACCCCATTAAAAAAACATTTATATTATTTTTATTTTAACATAGAATGTTTTTTTGTTTTCATATTTTTTTTATTAAATGGTTGGATTCAATATAATTGAAATTTTTTCCAATTATTTGGTGATTTTGTAGCTTAGAGGAACTCTAATAGCTCCAAGCAAGGGTGCATGGCCTAATGATAAAGGTAGATGGATCCAATTTTTTGATATTGATGGACTCACTGTTAAAGGACCAGGAGAAATTGATGCCCAAGGTTCTTCATGGTGGGAAGATTGCCATACACATTGTAAAAGACCAACAGTAAGTTTTTAATTCCTTTCATATTTGTATAAGTAATTTTGTGAAGATTTTTTTTTTCAATCACTTGATTATTGATTTTACTTCTAACTAGGGTTTGGTGGTTTAATTTGTAATGTGAATATTGATTATGAAATATTTGGTTTTTGTAGGCAGTGTTTTTCCACAATTGCAATGGACTCCAACTGAGGGAAATGAAACACGTCAACAGTGGCAAAAATCATATCTCCATAAATGTTTGCAATAATGTCATTGTCTCGGATATTCACATTATTGCTCCTGATGAAAGTCCTAACACTGATGGAATTGACATTTCTAGATCAAGCAATGTTCTTGTGCAAAATTCTTTAATGGAAACTGGTAATAAATTAGTATTATTCTCTTTAAGATTATTATTTTTGTTGATATATATAGTTTGAATTCATATAATTACACATTTATTTATATATATTTTTATTTCAATATAGGCGATGATTGTATTGCCATTAACAATGGAACTACCAATATCAAGATCGTAGGTGTCACTTGTGGCCCTGGCCATGGCATAAGGTAAAAAGAACATATTTGGTAACTCTTTATAATATAATATTTATAAAACATCTCATTTTTCATCTCTCATTTTTGTTTGTTTGTTTGATTGCAGCATTGGGAGTTTAGGGGAAGATGGAGAATACAGTGTAGTTGAAAATGTCTACGTAAGTGATTGCCTCTTAAAAGGCACTCAAAATGGAGTTAGAATCAAGACTTGGGAGGTATGTTGATTAGCGCCTTCAATTTATTCATATTCAACATTCAATAAAATTATAAACAAAATTAACTATTTTTCTTGAATTACAGGGTGGATACGGATATGCAAGAAATATCACGTTCGAGAAAATAACACTCGAGAGCACAAAAAACCCGATCATCATCGACCAATACTACACCGCGTATGCAAGTGCAAGGAAGAATAAGGTACAACGAATGAATTCCATCGACTTCTCGTCACATTTTTTGTTCATTAATATCTATTGATCTTTTATGTGTCGATTCTTTTTAGGCAATGGACATAAAAGTGAGCGATGTTACATATCGTGAAGTGAATGGAACATCAGCAAATGAAAATGCAATTACCTTGAACTGTTCTCGAGCTCGTTGCACCAATATTGTAATGGACAATGTCAACATAAGGACTTCAACTCCAGGGAAAGAAGCCAAAGCTTTTTGCCAAAATGTTGAAGGAAAATCTAAGTCTACAATAATCCCAACTGTGCCTTGCTTGTCAAAATCCCATTAA

mRNA sequence

ATGAGGCTCCATTTTGCTTTAGTTTTCGTCTTTTCCATTGCCATTTTGAGCTCATGGACTATATCTGCTTCGACTTACTTCAATGTTCTTGATTATGGAGCTGCTGGAAATGGCAAAACCGATGATTCAGAACTTAGAGGAACTCTAATAGCTCCAAGCAAGGGTGCATGGCCTAATGATAAAGGTAGATGGATCCAATTTTTTGATATTGATGGACTCACTGTTAAAGGACCAGGAGAAATTGATGCCCAAGGTTCTTCATGGTGGGAAGATTGCCATACACATTGTAAAAGACCAACAGCAGTGTTTTTCCACAATTGCAATGGACTCCAACTGAGGGAAATGAAACACGTCAACAGTGGCAAAAATCATATCTCCATAAATGTTTGCAATAATGTCATTGTCTCGGATATTCACATTATTGCTCCTGATGAAAGTCCTAACACTGATGGAATTGACATTTCTAGATCAAGCAATGTTCTTGTGCAAAATTCTTTAATGGAAACTGGCGATGATTGTATTGCCATTAACAATGGAACTACCAATATCAAGATCGTAGGTGTCACTTGTGGCCCTGGCCATGGCATAAGCATTGGGAGTTTAGGGGAAGATGGAGAATACAGTGTAGTTGAAAATGTCTACGTAAGTGATTGCCTCTTAAAAGGCACTCAAAATGGAGTTAGAATCAAGACTTGGGAGGGTGGATACGGATATGCAAGAAATATCACGTTCGAGAAAATAACACTCGAGAGCACAAAAAACCCGATCATCATCGACCAATACTACACCGCGTATGCAAGTGCAAGGAAGAATAAGGCAATGGACATAAAAGTGAGCGATGTTACATATCGTGAAGTGAATGGAACATCAGCAAATGAAAATGCAATTACCTTGAACTGTTCTCGAGCTCGTTGCACCAATATTGTAATGGACAATGTCAACATAAGGACTTCAACTCCAGGGAAAGAAGCCAAAGCTTTTTGCCAAAATGTTGAAGGAAAATCTAAGTCTACAATAATCCCAACTGTGCCTTGCTTGTCAAAATCCCATTAA

Coding sequence (CDS)

ATGAGGCTCCATTTTGCTTTAGTTTTCGTCTTTTCCATTGCCATTTTGAGCTCATGGACTATATCTGCTTCGACTTACTTCAATGTTCTTGATTATGGAGCTGCTGGAAATGGCAAAACCGATGATTCAGAACTTAGAGGAACTCTAATAGCTCCAAGCAAGGGTGCATGGCCTAATGATAAAGGTAGATGGATCCAATTTTTTGATATTGATGGACTCACTGTTAAAGGACCAGGAGAAATTGATGCCCAAGGTTCTTCATGGTGGGAAGATTGCCATACACATTGTAAAAGACCAACAGCAGTGTTTTTCCACAATTGCAATGGACTCCAACTGAGGGAAATGAAACACGTCAACAGTGGCAAAAATCATATCTCCATAAATGTTTGCAATAATGTCATTGTCTCGGATATTCACATTATTGCTCCTGATGAAAGTCCTAACACTGATGGAATTGACATTTCTAGATCAAGCAATGTTCTTGTGCAAAATTCTTTAATGGAAACTGGCGATGATTGTATTGCCATTAACAATGGAACTACCAATATCAAGATCGTAGGTGTCACTTGTGGCCCTGGCCATGGCATAAGCATTGGGAGTTTAGGGGAAGATGGAGAATACAGTGTAGTTGAAAATGTCTACGTAAGTGATTGCCTCTTAAAAGGCACTCAAAATGGAGTTAGAATCAAGACTTGGGAGGGTGGATACGGATATGCAAGAAATATCACGTTCGAGAAAATAACACTCGAGAGCACAAAAAACCCGATCATCATCGACCAATACTACACCGCGTATGCAAGTGCAAGGAAGAATAAGGCAATGGACATAAAAGTGAGCGATGTTACATATCGTGAAGTGAATGGAACATCAGCAAATGAAAATGCAATTACCTTGAACTGTTCTCGAGCTCGTTGCACCAATATTGTAATGGACAATGTCAACATAAGGACTTCAACTCCAGGGAAAGAAGCCAAAGCTTTTTGCCAAAATGTTGAAGGAAAATCTAAGTCTACAATAATCCCAACTGTGCCTTGCTTGTCAAAATCCCATTAA

Protein sequence

MRLHFALVFVFSIAILSSWTISASTYFNVLDYGAAGNGKTDDSELRGTLIAPSKGAWPNDKGRWIQFFDIDGLTVKGPGEIDAQGSSWWEDCHTHCKRPTAVFFHNCNGLQLREMKHVNSGKNHISINVCNNVIVSDIHIIAPDESPNTDGIDISRSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGPGHGISIGSLGEDGEYSVVENVYVSDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLESTKNPIIIDQYYTAYASARKNKAMDIKVSDVTYREVNGTSANENAITLNCSRARCTNIVMDNVNIRTSTPGKEAKAFCQNVEGKSKSTIIPTVPCLSKSH

Homology

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BLAST of Spg002942 vs. NCBI nr
Match: XP_038888688.1 (probable polygalacturonase At3g15720 [Benincasa hispida])

HSP 1 Score: 532.3 bits (1370), Expect = 3.1e-147
Identity = 266/396 (67.17%), Postives = 305/396 (77.02%), Query Frame = 0


Query: 1   MRLHFALVFVFSIAILSSWTISAST-YFNVLDYGAAGNGKTDDS---------------- 60
           M+L F  V +FSIA LSS  + AS  YFNVL YGA GN +TDDS                
Sbjct: 1   MKLLFVFVSIFSIANLSSRNVLASPHYFNVLHYGATGNDETDDSQAFLKAWKDVCEATKG 60

Query: 61  ------------------------------ELRGTLIAPSKGAWPND-KGRWIQFFDIDG 120
                                         +LRGTL+AP KGAWP D K +WI+F DIDG
Sbjct: 61  IPTLHVPLGKSYLLNPLTFQGPCKSKQVNIQLRGTLVAPKKGAWPTDKKDKWIEFHDIDG 120

Query: 121 LTVKGPGEIDAQGSSWWEDCHTHCKRPTAVFFHNCNGLQLREMKHVNSGKNHISINVCNN 180
           LT+ G G+ID QGSSWWEDC THC+RPTA+FFHNCNG  LR+MKHVNSG+NHISIN CNN
Sbjct: 121 LTINGGGQIDGQGSSWWEDCETHCERPTALFFHNCNGFLLRDMKHVNSGRNHISINACNN 180

Query: 181 VIVSDIHIIAPDESPNTDGIDISRSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGP 240
           V+VS+IHIIAP+ SPNTDGIDI+RS+NV +Q S M TGDDC+AINNG++NI IVG+TCGP
Sbjct: 181 VMVSNIHIIAPEASPNTDGIDIARSTNVFIQKSFMATGDDCVAINNGSSNINIVGITCGP 240

Query: 241 GHGISIGSLGEDGEYSVVENVYVSDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLEST 300
           GHGISIGSLG DG+Y+VVE+V+V +C  KG QNGVRIKTWEGGYGYARNITFEKITL +T
Sbjct: 241 GHGISIGSLGADGQYNVVEDVHVRNCFFKGAQNGVRIKTWEGGYGYARNITFEKITLMNT 300

Query: 301 KNPIIIDQYYTAYASARKNKAMDIKVSDVTYREVNGTSANENAITLNCSRARCTNIVMDN 349
           +NPIIIDQYYT  AS+RKNK ++IKVSDVTYREV+GTSANENAITLNCSRARCTNI+MDN
Sbjct: 301 RNPIIIDQYYTTSASSRKNKVINIKVSDVTYREVHGTSANENAITLNCSRARCTNILMDN 360

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BLAST of Spg002942 vs. NCBI nr
Match: XP_023552838.1 (probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 526.9 bits (1356), Expect = 1.3e-145
Identity = 259/398 (65.08%), Postives = 309/398 (77.64%), Query Frame = 0


Query: 1   MRLHFALVFVFSIAILSSWTISASTYFNVLDYGAAGNGKTDDSE---------------- 60
           M+L FA++F+F + +L S T+SA +YFNVLDYGA GNGKTDDSE                
Sbjct: 1   MKLLFAVIFLFYVTVLRSLTVSALSYFNVLDYGATGNGKTDDSEAFLKAWNDACAVIEDN 60

Query: 61  ------------------------------LRGTLIAPSKGAWPND-KGRWIQFFDIDGL 120
                                         L G L+APSK  WP+D + +W+QF++ID L
Sbjct: 61  PTLRVPFGKTYLLKPLKFQGPCKSKQVNFDLGGALMAPSKDEWPSDGEDKWVQFYNIDSL 120

Query: 121 TVKGPGEIDAQGSSWW-EDCHTHCKRPTAVFFHNCNGLQLREMKHVNSGKNHISINVCNN 180
           TV+G G  + QGSSWW +DCH HC RPTA+FFHNCNGL+L +MKH+NSGKNHISIN CNN
Sbjct: 121 TVQGDGRFNGQGSSWWNKDCHNHCNRPTALFFHNCNGLRLSKMKHINSGKNHISINDCNN 180

Query: 181 VIVSDIHIIAPDESPNTDGIDISRSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGP 240
           V++SDIHI AP++SPNTDGIDIS+S NVL+QNSL+ TGDDCIAINNG++NI IVG+TCGP
Sbjct: 181 VVISDIHISAPEQSPNTDGIDISQSKNVLIQNSLIATGDDCIAINNGSSNINIVGITCGP 240

Query: 241 GHGISIGSLGEDGEYSVVENVYVSDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLEST 300
           GHGISIGSLG+DG+Y+VVEN+YV +C LK TQNGVRIKTWEGGYGYARNITFEKI LE+T
Sbjct: 241 GHGISIGSLGKDGDYNVVENIYVRNCHLKDTQNGVRIKTWEGGYGYARNITFEKIILENT 300

Query: 301 KNPIIIDQYYTAYASARKNKAMDIKVSDVTYREVNGTSANENAITLNCSRARCTNIVMDN 351
           KNPIIIDQYY+A+A ARKN   +IKVS+VTYREV+GTSANENAI LNCSRARCTNI+M+N
Sbjct: 301 KNPIIIDQYYSAFAYARKNMGREIKVSNVTYREVHGTSANENAIILNCSRARCTNIIMNN 360

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BLAST of Spg002942 vs. NCBI nr
Match: XP_038888676.1 (probable polygalacturonase At3g15720 [Benincasa hispida])

HSP 1 Score: 518.1 bits (1333), Expect = 6.0e-143
Identity = 257/398 (64.57%), Postives = 309/398 (77.64%), Query Frame = 0


Query: 1   MRLHFALVFVFSIAILSSWTISASTYFNVLDYGAAGNGKTDDSE---------------- 60
           M+L FAL+F+F+I IL+   +SAS+ FNVLDYGA GNGK DDSE                
Sbjct: 1   MKLLFALIFIFTIDILTLSIVSASSNFNVLDYGAIGNGKADDSEAFLKAWNDVCGATEDS 60

Query: 61  ------------------------------LRGTLIAPSKGAWPND-KGRWIQFFDIDGL 120
                                         LRGTL+APSK  WP+D   +W+QF+DI+GL
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSNQVNFDLRGTLMAPSKDEWPSDGADKWVQFYDIEGL 120

Query: 121 TVKGPGEIDAQGSSWWEDC-HTHCKRPTAVFFHNCNGLQLREMKHVNSGKNHISINVCNN 180
            +KG G+ D QGS WW+DC   HCKRPTA+FFHNCNGLQ++EMKH+NSG+NHISIN CN+
Sbjct: 121 NLKGAGKFDGQGSLWWKDCDRKHCKRPTALFFHNCNGLQVKEMKHINSGRNHISINDCND 180

Query: 181 VIVSDIHIIAPDESPNTDGIDISRSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGP 240
           VI S+I I+AP+ESPNTDGIDIS+S NVL+Q+S + TGDDCIAIN G+++I I+G+TCGP
Sbjct: 181 VIFSNIQILAPEESPNTDGIDISKSKNVLIQDSFIATGDDCIAINEGSSDINIIGITCGP 240

Query: 241 GHGISIGSLGEDGEYSVVENVYVSDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLEST 300
           GHGISIGSLG+DG Y+VVEN++VS+CLL+GTQNGVRIKTW+GGYGYA+NITFEKITL++ 
Sbjct: 241 GHGISIGSLGKDGAYNVVENIHVSNCLLQGTQNGVRIKTWQGGYGYAKNITFEKITLKNA 300

Query: 301 KNPIIIDQYYTAYASARKNKAMDIKVSDVTYREVNGTSANENAITLNCSRARCTNIVMDN 351
           KNPIIIDQYYT+YA +RK K MDIKVSDVTYREVNGTSANE+AITLNCSRARCTNI++DN
Sbjct: 301 KNPIIIDQYYTSYAYSRKIKGMDIKVSDVTYREVNGTSANEDAITLNCSRARCTNIILDN 360

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BLAST of Spg002942 vs. NCBI nr
Match: XP_022141108.1 (probable polygalacturonase At3g15720 [Momordica charantia])

HSP 1 Score: 515.0 bits (1325), Expect = 5.1e-142
Identity = 251/397 (63.22%), Postives = 302/397 (76.07%), Query Frame = 0


Query: 1   MRLHFALVFVFSIAILSSWTISASTYFNVLDYGAAGNGKTDDSE---------------- 60
           M+LH ALVF+FSI +LSSW +S S  FNVLDYGA GNG+TDDS+                
Sbjct: 1   MKLHLALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQAFLRAWXDACGATEGI 60

Query: 61  ------------------------------LRGTLIAPSKGAWP-NDKGRWIQFFDIDGL 120
                                         L GT++AP+K +WP +D+ RWIQF DIDGL
Sbjct: 61  PALHAPVGKIYMLNPLKFQGPCKSKQVIFHLGGTVVAPTKDSWPSDDRDRWIQFSDIDGL 120

Query: 121 TVKGPGEIDAQGSSWWEDCHTHCKRPTAVFFHNCNGLQLREMKHVNSGKNHISINVCNNV 180
            V G G+ D QGSSWW+DC  HC RPTA+F HNCNGLQLREMKH+NSG+NHISIN CN+ 
Sbjct: 121 MVNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACNDA 180

Query: 181 IVSDIHIIAPDESPNTDGIDISRSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGPG 240
           I+SD+HI+AP++SPNTDGIDIS S+NVL+QNS M TGDDCIA+NNG+TNI IVGVTCGPG
Sbjct: 181 IISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPG 240

Query: 241 HGISIGSLGEDGEYSVVENVYVSDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLESTK 300
           HGISIGSLGE+G+Y+VVE+++V +CLLKGTQNGVRIKTW+ GYGYAR ITFE+ITL   K
Sbjct: 241 HGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVK 300

Query: 301 NPIIIDQYYTAYASARKNKAMDIKVSDVTYREVNGTSANENAITLNCSRARCTNIVMDNV 351
           NPIIIDQYY+  A A+KN+ + IKVSDVTYREV+GTSANENAI LNCSR+RC+NI+M+NV
Sbjct: 301 NPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSRCSNIIMENV 360

[Close]

BLAST of Spg002942 vs. NCBI nr
Match: XP_022979578.1 (probable polygalacturonase At3g15720 [Cucurbita maxima])

HSP 1 Score: 515.0 bits (1325), Expect = 5.1e-142
Identity = 258/398 (64.82%), Postives = 302/398 (75.88%), Query Frame = 0


Query: 1   MRLHFALVFVFSIAILSSWTISASTYFNVLDYGAAGNGKTDDSE---------------- 60
           M+L  + VF+FS A+LSS T S S+YFNVLDYGA GNGK DDSE                
Sbjct: 1   MKLLVSFVFLFSTAVLSSLTESTSSYFNVLDYGANGNGKKDDSEAFLKAWNDACASTEDN 60

Query: 61  ------------------------------LRGTLIAPSKGAWPND-KGRWIQFFDIDGL 120
                                         L G ++APSK  W ND   +WIQF+DID L
Sbjct: 61  PTLRVPFGKTFLLNPLKFQGPCKSERINFDLGGIVMAPSKDEWSNDGVDKWIQFYDIDSL 120

Query: 121 TVKGPGEIDAQGSSWWE-DCHTHCKRPTAVFFHNCNGLQLREMKHVNSGKNHISINVCNN 180
           TVKG G  D QGSSWW+ DCH HCKRPTA+FFHNCNGL L + KHVNSG+NH+SIN CN+
Sbjct: 121 TVKGDGRFDGQGSSWWDKDCHKHCKRPTALFFHNCNGLHLNKTKHVNSGRNHMSINNCND 180

Query: 181 VIVSDIHIIAPDESPNTDGIDISRSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGP 240
           VI+SDIHI APDESPNTDGIDIS S NV +Q+S + TGDDC+AIN+G++NI I+G+TCGP
Sbjct: 181 VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDIIGLTCGP 240

Query: 241 GHGISIGSLGEDGEYSVVENVYVSDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLEST 300
           GHGISIGSLG+DG+Y+VVENVYVSDC+L+ TQNGVRIKTWEGGYGYA+NITFEKITLE+ 
Sbjct: 241 GHGISIGSLGKDGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300

Query: 301 KNPIIIDQYYTAYASARKNKAMDIKVSDVTYREVNGTSANENAITLNCSRARCTNIVMDN 351
           KNPIIIDQ+YTAYA  RKNK  +IKVSDVTYR+V+GTSANE AITL+CSRARCTNI+M+ 
Sbjct: 301 KNPIIIDQHYTAYAYRRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEE 360

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BLAST of Spg002942 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 4.7e-82
Identity = 172/389 (44.22%), Postives = 229/389 (58.87%), Query Frame = 0


Query: 11  FSIAILSSWTISASTYFNVLDYGAAGNGKTDDS--------------------------- 70
           FS+  L  +T  +S   +V  +GA G+G TDDS                           
Sbjct: 10  FSLFFLQIFT--SSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDGQFVVPAGMTF 69

Query: 71  ------------------ELRGTLIAPSKGAWPNDKGRWIQFFDIDGLTVKGPGEIDAQG 130
                             ++ G L+APSKG W  DK +WI F DI+GL ++G GEI+ QG
Sbjct: 70  MLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKGDKDQWILFTDIEGLVIEGDGEINGQG 129

Query: 131 SSWWEDCHTHCKRPTAVFFHNCNGLQLREMKHVNSGKNHISINVCNNVIVSDIHIIAPDE 190
           SSWWE       RPTA+ F +CN L+L  + H++S   HI I+ CN V +S + I AP+ 
Sbjct: 130 SSWWEH---KGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPES 189

Query: 191 SPNTDGIDISRSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGPGHGISIGSLGEDG 250
           SPNTDGID+  SSNV++Q+ ++ TGDDCIAIN+GT+NI I G+ CGPGHGISIGSLG+DG
Sbjct: 190 SPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDG 249

Query: 251 EYSVVENVYVSDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLESTKNPIIIDQYYTAY 310
           E + VENV V +C  +GT NG RIKTW+GG GYAR ITF  ITL++ +NPIIIDQ+Y   
Sbjct: 250 ETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGG 309

Query: 311 AS--ARKNKAMDIKVSDVTYREVNGTSANENAITLNCS-RARCTNIVMDNVNIRT--STP 350
            S  A+  K+  ++VS V +    GTS +E  +   CS R  CT I + ++ I T  S  
Sbjct: 310 DSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGS 369

[Close]

BLAST of Spg002942 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 248.4 bits (633), Expect = 1.2e-64
Identity = 146/378 (38.62%), Postives = 207/378 (54.76%), Query Frame = 0


Query: 20  TISASTYFNVLDYGAAGNGKTDDS------------------------------------ 79
           ++   T  +V D+GA G+GKTDD+                                    
Sbjct: 61  SLRTPTTVSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSIQLTG 120

Query: 80  --------ELRGTLIAPSKGAWPNDKGRWIQFFDIDGLTVKG--PGEIDAQGSSWWEDCH 139
                   ++ GTL A  K +   D  +WI F  ++ L+V G   G +D  G +WW++  
Sbjct: 121 PCNSILTVQIFGTLSASQKRSDYKDISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQN-- 180

Query: 140 THCKR---------PTAVFFHNCNGLQLREMKHVNSGKNHISINVCNNVIVSDIHIIAPD 199
             CKR         PTA+ F+N   L ++ +K  N+ +  ISI  C+NV VS++ + AP 
Sbjct: 181 -SCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPA 240

Query: 200 ESPNTDGIDISRSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGPGHGISIGSLGED 259
           +SPNTDGI I+ + N+ V  S++ TGDDCI+I +G+ N++I  +TCGPGHGISIGSLG+D
Sbjct: 241 DSPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDD 300

Query: 260 GEYSVVENVYVSDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLESTKNPIIIDQYYTA 319
              + V  V V    L GT NGVRIKT++GG G A NI F+ I +++ KNPIIIDQ Y  
Sbjct: 301 NSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCD 360

Query: 320 YASARKNKAMDIKVSDVTYREVNGTSANENAITLNCSR-ARCTNIVMDNVNIRTSTPGKE 342
            +     K+  ++V +V YR+++GTSA+ENAIT NCS+   C  IV+D VNI      K 
Sbjct: 361 KSKCTTEKSA-VQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNI------KG 420

[Close]

BLAST of Spg002942 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.5e-61
Identity = 120/313 (38.34%), Postives = 183/313 (58.47%), Query Frame = 0


Query: 44  ELRGTLIAPS-KGAWPN-DKGRWIQFFDIDGLTVKGPGEIDAQGSSWWE-DC-HTH---C 103
           ++ GT+IAP+    W   ++ +W+ F  +  LTV+G G ++  G  WWE  C H H   C
Sbjct: 114 QISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSNPC 173

Query: 104 K-RPTAVFFHNCNGLQLREMKHVNSGKNHISINVCNNVIVSDIHIIAPDESPNTDGIDIS 163
           +  PTA+ FH C  +++  +  ++S + HI++  C  V +S + +IAP  SPNTDGI IS
Sbjct: 174 RGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHIS 233

Query: 164 RSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGPGHGISIGSLGEDGEYSVVENVYV 223
            S  +++ N+ + TGDDCI+I   +T I I  + CGPGHGISIGSLG+   +  V ++ V
Sbjct: 234 VSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITV 293

Query: 224 SDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLESTKNPIIIDQYYTAYASARKNKAMD 283
              ++  T NGVRIKTW+GG G    I F  I + +  NPIIIDQYY        N+   
Sbjct: 294 DTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSA 353

Query: 284 IKVSDVTYREVNGTSANENAITLNCS-RARCTNIVMDNVNIRTSTPGKEAKAFCQNVEGK 343
           I + ++++  V GTSA++ AI ++CS  + C NI++ ++++  S      ++FC    G 
Sbjct: 354 ISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEAYGS 413

Query: 344 SKSTIIPTVPCLS 348
           S   + P  PCLS
Sbjct: 414 SSGQVYPP-PCLS 425

[Close]

BLAST of Spg002942 vs. ExPASy Swiss-Prot
Match: Q9SFB7 (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 236.5 bits (602), Expect = 4.6e-61
Identity = 121/298 (40.60%), Postives = 181/298 (60.74%), Query Frame = 0


Query: 56  AWPN-----DKGRWIQFFDIDGLTVKGPGEIDAQGSSWW-EDCHTHCK-----RPTAVFF 115
           AW N     ++  WI F +++ L V+G G ID  G  WW + C  + +      PTAV F
Sbjct: 139 AWENPSDYKERRHWIVFENVNNLRVEGGGRIDGNGHIWWPKSCKINPQLPCLGAPTAVTF 198

Query: 116 HNCNGLQLREMKHVNSGKNHISINVCNNVIVSDIHIIAPDESPNTDGIDISRSSNVLVQN 175
             CN L++  ++  N+ + H++   C NV   ++ + +P +SPNTDGI +S + N+L+Q+
Sbjct: 199 VECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPADSPNTDGIHVSGTQNILIQD 258

Query: 176 SLMETGDDCIAINNGTTNIKIVGVTCGPGHGISIGSLGEDGEYSVVENVYVSDCLLKGTQ 235
           S++ TGDDCI+I +G+ N++  G+TCGPGHGISIGSLGED   + V NV V+   L GT 
Sbjct: 259 SIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGEDNSEAYVSNVVVNKATLIGTT 318

Query: 236 NGVRIKTWEGGYGYARNITFEKITLESTKNPIIIDQYYTAYASARKNKAMDIKVSDVTYR 295
           NGVRIKTW+GG+G A+NI F+ I +++  NPIII+Q Y     A   +   ++VS+V Y+
Sbjct: 319 NGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCDRVEACPEQKSAVQVSNVLYK 378

Query: 296 EVNGTSANENAITLNCSR-ARCTNIVMDNVNIRTSTPGKEAKAFCQNVEGKSKSTIIP 342
            + GTS+   A+   CS+   C  I M NV +   T    +KA C NV+  ++  + P
Sbjct: 379 NIQGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQTQQDVSKASCSNVKLDTRGNVSP 436

[Close]

BLAST of Spg002942 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.3e-60
Identity = 146/389 (37.53%), Postives = 211/389 (54.24%), Query Frame = 0


Query: 10  VFSIAILSSWTISASTYFNVLDYGAAGNGKTDDS-------------------------- 69
           V S+      T+ AST  +V ++GA G+GKTDD+                          
Sbjct: 52  VLSLISSDETTLEAST-VSVSNFGAKGDGKTDDTQAFKKAWKKACSTNGVTTFLVPKGKT 111

Query: 70  ------------------ELRGTLIAPSKGAWPNDKGRWIQFFDIDGLTVKG--PGEIDA 129
                             ++ GTL A +K +   DK  W+   D++ L++ G   G I+ 
Sbjct: 112 YLLKSTRFRGPCKSLRNFQILGTLSASTKRSDYKDKNHWLILEDVNNLSIDGGSTGIING 171

Query: 130 QGSSWWE-----DCHTHC-KRPTAVFFHNCNGLQLREMKHVNSGKNHISINVCNNVIVSD 189
            G +WW+     D    C K PTA+  +N   L ++ ++  N+ +  ISI  CN V VS+
Sbjct: 172 NGKTWWQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSN 231

Query: 190 IHIIAPDESPNTDGIDISRSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGPGHGIS 249
           + I AP +SPNTDGI I+ + N+ V NS + TGDDCI+I +GT N++I  +TCGPGHGIS
Sbjct: 232 VEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGIS 291

Query: 250 IGSLGEDGEYSVVENVYVSDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLESTKNPII 309
           IGSLG+D   + V  + V       + NGVRIKT++GG G A+NI F+ I +E+ KNPII
Sbjct: 292 IGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENVKNPII 351

Query: 310 IDQYYTAYASARKNKAMD----IKVSDVTYREVNGTSANENAITLNCS-RARCTNIVMDN 342
           IDQ Y       K+K  D    ++V +V Y+ ++GTSA + AITLNCS +  C  IV++N
Sbjct: 352 IDQDY-----CDKDKCEDQESAVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIVLEN 411

[Close]

BLAST of Spg002942 vs. ExPASy TrEMBL
Match: A0A6J1IWN4 (probable polygalacturonase At3g15720 OS=Cucurbita maxima OX=3661 GN=LOC111479259 PE=3 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 2.5e-142
Identity = 258/398 (64.82%), Postives = 302/398 (75.88%), Query Frame = 0


Query: 1   MRLHFALVFVFSIAILSSWTISASTYFNVLDYGAAGNGKTDDSE---------------- 60
           M+L  + VF+FS A+LSS T S S+YFNVLDYGA GNGK DDSE                
Sbjct: 1   MKLLVSFVFLFSTAVLSSLTESTSSYFNVLDYGANGNGKKDDSEAFLKAWNDACASTEDN 60

Query: 61  ------------------------------LRGTLIAPSKGAWPND-KGRWIQFFDIDGL 120
                                         L G ++APSK  W ND   +WIQF+DID L
Sbjct: 61  PTLRVPFGKTFLLNPLKFQGPCKSERINFDLGGIVMAPSKDEWSNDGVDKWIQFYDIDSL 120

Query: 121 TVKGPGEIDAQGSSWWE-DCHTHCKRPTAVFFHNCNGLQLREMKHVNSGKNHISINVCNN 180
           TVKG G  D QGSSWW+ DCH HCKRPTA+FFHNCNGL L + KHVNSG+NH+SIN CN+
Sbjct: 121 TVKGDGRFDGQGSSWWDKDCHKHCKRPTALFFHNCNGLHLNKTKHVNSGRNHMSINNCND 180

Query: 181 VIVSDIHIIAPDESPNTDGIDISRSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGP 240
           VI+SDIHI APDESPNTDGIDIS S NV +Q+S + TGDDC+AIN+G++NI I+G+TCGP
Sbjct: 181 VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDIIGLTCGP 240

Query: 241 GHGISIGSLGEDGEYSVVENVYVSDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLEST 300
           GHGISIGSLG+DG+Y+VVENVYVSDC+L+ TQNGVRIKTWEGGYGYA+NITFEKITLE+ 
Sbjct: 241 GHGISIGSLGKDGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300

Query: 301 KNPIIIDQYYTAYASARKNKAMDIKVSDVTYREVNGTSANENAITLNCSRARCTNIVMDN 351
           KNPIIIDQ+YTAYA  RKNK  +IKVSDVTYR+V+GTSANE AITL+CSRARCTNI+M+ 
Sbjct: 301 KNPIIIDQHYTAYAYRRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEE 360

[Close]

BLAST of Spg002942 vs. ExPASy TrEMBL
Match: A0A6J1CJJ6 (probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011582 PE=3 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 2.5e-142
Identity = 251/397 (63.22%), Postives = 302/397 (76.07%), Query Frame = 0


Query: 1   MRLHFALVFVFSIAILSSWTISASTYFNVLDYGAAGNGKTDDSE---------------- 60
           M+LH ALVF+FSI +LSSW +S S  FNVLDYGA GNG+TDDS+                
Sbjct: 1   MKLHLALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQAFLRAWXDACGATEGI 60

Query: 61  ------------------------------LRGTLIAPSKGAWP-NDKGRWIQFFDIDGL 120
                                         L GT++AP+K +WP +D+ RWIQF DIDGL
Sbjct: 61  PALHAPVGKIYMLNPLKFQGPCKSKQVIFHLGGTVVAPTKDSWPSDDRDRWIQFSDIDGL 120

Query: 121 TVKGPGEIDAQGSSWWEDCHTHCKRPTAVFFHNCNGLQLREMKHVNSGKNHISINVCNNV 180
            V G G+ D QGSSWW+DC  HC RPTA+F HNCNGLQLREMKH+NSG+NHISIN CN+ 
Sbjct: 121 MVNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACNDA 180

Query: 181 IVSDIHIIAPDESPNTDGIDISRSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGPG 240
           I+SD+HI+AP++SPNTDGIDIS S+NVL+QNS M TGDDCIA+NNG+TNI IVGVTCGPG
Sbjct: 181 IISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPG 240

Query: 241 HGISIGSLGEDGEYSVVENVYVSDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLESTK 300
           HGISIGSLGE+G+Y+VVE+++V +CLLKGTQNGVRIKTW+ GYGYAR ITFE+ITL   K
Sbjct: 241 HGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVK 300

Query: 301 NPIIIDQYYTAYASARKNKAMDIKVSDVTYREVNGTSANENAITLNCSRARCTNIVMDNV 351
           NPIIIDQYY+  A A+KN+ + IKVSDVTYREV+GTSANENAI LNCSR+RC+NI+M+NV
Sbjct: 301 NPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSRCSNIIMENV 360

[Close]

BLAST of Spg002942 vs. ExPASy TrEMBL
Match: A0A0A0KZ95 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G025750 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.3e-140
Identity = 248/394 (62.94%), Postives = 305/394 (77.41%), Query Frame = 0


Query: 1   MRLHFALVFVFSIAILSSWTISASTYFNVLDYGAAGNGKTDDS----------------- 60
           M+L  A +F++SIAILSSWT+  S+ FNVLDYGA GNGKTDDS                 
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 61  -------------------------ELRGTLIAPSKGAWPND-KGRWIQFFDIDGLTVKG 120
                                    +L GTL+AP+K  W +D KG+WIQFFDI+GL ++G
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSKQLGGTLMAPTKDEWISDGKGQWIQFFDIEGLNLQG 120

Query: 121 PGEIDAQGSSWWEDC-HTHCKRPTAVFFHNCNGLQLREMKHVNSGKNHISINVCNNVIVS 180
            G  D QGS WW+DC H HCKRPTA+FFHNCNGLQ++ MKH+NS KNHISIN+C++VI S
Sbjct: 121 GGRFDGQGSLWWKDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHDVIFS 180

Query: 181 DIHIIAPDESPNTDGIDISRSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGPGHGI 240
            +HI AP++SPNTDGIDIS S+N+ +++S M TGDDCIAINNG++NI I G+TCGPGHGI
Sbjct: 181 HLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGPGHGI 240

Query: 241 SIGSLGEDGEYSVVENVYVSDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLESTKNPI 300
           SIGSLG+DGEY+VVENV+VS+CLL+ TQNG+RIKTWEGGYGYA+NITFEKIT+++ KNPI
Sbjct: 241 SIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNAKNPI 300

Query: 301 IIDQYYTAYASARKNKAMDIKVSDVTYREVNGTSANENAITLNCSRARCTNIVMDNVNIR 351
           IIDQYY++YA +RK K MDIKVSDVTYREVNGTSANE+AIT NCS+ARC+NI++DNVNI+
Sbjct: 301 IIDQYYSSYAYSRKMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILDNVNIK 360

[Close]

BLAST of Spg002942 vs. ExPASy TrEMBL
Match: A0A6J1GVS6 (probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC111457994 PE=3 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 8.7e-140
Identity = 254/398 (63.82%), Postives = 302/398 (75.88%), Query Frame = 0


Query: 1   MRLHFALVFVFSIAILSSWTISASTYFNVLDYGAAGNGKTDDSE---------------- 60
           M+L  + VF+F I +LSS T SAS+YFNVLDYGA GNGKTDDSE                
Sbjct: 1   MKLFVSFVFLFFIVVLSSLTESASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDN 60

Query: 61  ------------------------------LRGTLIAPSKGAWPND-KGRWIQFFDIDGL 120
                                         L G ++APSK  W +D   +WIQF+DID L
Sbjct: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSL 120

Query: 121 TVKGPGEIDAQGSSWWE-DCHTHCKRPTAVFFHNCNGLQLREMKHVNSGKNHISINVCNN 180
           TVKG G+ D QGSSWW+ DCH HCKRPTA+FFHNCN L L + KHVNSG+NH+SIN CN+
Sbjct: 121 TVKGDGQFDGQGSSWWDKDCHKHCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCND 180

Query: 181 VIVSDIHIIAPDESPNTDGIDISRSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGP 240
           VI+SDIHI AP+ESPNTDGIDIS S NV + +S + TGDDCIAIN+G++NI I+G+TCGP
Sbjct: 181 VIISDIHITAPNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGP 240

Query: 241 GHGISIGSLGEDGEYSVVENVYVSDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLEST 300
           GHGISIGSLG++G+Y+VVENVYVS+C+L+ TQNGVRIKTWEGGYGYA+NITFEKITLE+ 
Sbjct: 241 GHGISIGSLGKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300

Query: 301 KNPIIIDQYYTAYASARKNKAMDIKVSDVTYREVNGTSANENAITLNCSRARCTNIVMDN 351
           KNPIIIDQ+YTAYA  RKNK  +IKVSDVTYR+V+GTSANE AITL+CSRARCTNI+M+ 
Sbjct: 301 KNPIIIDQHYTAYAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEE 360

[Close]

BLAST of Spg002942 vs. ExPASy TrEMBL
Match: A0A6J1CIX8 (probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011581 PE=3 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.3e-138
Identity = 252/394 (63.96%), Postives = 298/394 (75.63%), Query Frame = 0


Query: 1   MRLHFALVFVFSIAILSSWTISASTYFNVLDYGAAGNGKTDDSE---------------- 60
           M+LHF L+F+FSI ILSSW +SAS  FNVLDYGA GNG+ DDS+                
Sbjct: 1   MKLHFVLLFIFSINILSSWIVSASPSFNVLDYGAIGNGQADDSQAFLRAWNDVCGATEGI 60

Query: 61  ------------------------------LRGTLIAPSKGAWPN-DKGRWIQFFDIDGL 120
                                         L+GTL+AP KG+WP  D+ RWIQF DIDGL
Sbjct: 61  PTLHAPHGKIYMLKPLKFQGPCKSKQVTFHLKGTLVAPKKGSWPKYDRERWIQFSDIDGL 120

Query: 121 TVKGPGEIDAQGSSWWEDCHTHCKRPTAVFFHNCNGLQLREMKHVNSGKNHISINVCNNV 180
            V G G+ D QGSSWWED   HC+RPTA+F HNCNGLQLREMKH+NS KNHISIN CN+V
Sbjct: 121 IVNGTGQFDGQGSSWWEDWE-HCQRPTALFLHNCNGLQLREMKHINSAKNHISINACNDV 180

Query: 181 IVSDIHIIAPDESPNTDGIDISRSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGPG 240
           I+SD+HIIAP++SPNTDGID+SRS+NVL+QNS M TGDDCIA+NNG++NI IVGVTCGPG
Sbjct: 181 IISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPG 240

Query: 241 HGISIGSLGEDGEYSVVENVYVSDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLESTK 300
           HGISIGSLG + EY+VVEN++V +CLLKGTQNG+RIKTW+GGYGYARNITFE+ITL   K
Sbjct: 241 HGISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAK 300

Query: 301 NPIIIDQYYTAYASARKNKAMDIKVSDVTYREVNGTSANENAITLNCSRARCTNIVMDNV 348
           NPIIIDQYYT + S    +  DIKVS+VTYREVNGTSANENAI LNCSR RC+NI+++NV
Sbjct: 301 NPIIIDQYYTDFFSI-FFQLFDIKVSNVTYREVNGTSANENAIILNCSRNRCSNIILENV 360

[Close]

BLAST of Spg002942 vs. TAIR 10
Match: AT3G15720.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 306.2 bits (783), Expect = 3.3e-83
Identity = 172/389 (44.22%), Postives = 229/389 (58.87%), Query Frame = 0


Query: 11  FSIAILSSWTISASTYFNVLDYGAAGNGKTDDS--------------------------- 70
           FS+  L  +T  +S   +V  +GA G+G TDDS                           
Sbjct: 10  FSLFFLQIFT--SSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDGQFVVPAGMTF 69

Query: 71  ------------------ELRGTLIAPSKGAWPNDKGRWIQFFDIDGLTVKGPGEIDAQG 130
                             ++ G L+APSKG W  DK +WI F DI+GL ++G GEI+ QG
Sbjct: 70  MLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKGDKDQWILFTDIEGLVIEGDGEINGQG 129

Query: 131 SSWWEDCHTHCKRPTAVFFHNCNGLQLREMKHVNSGKNHISINVCNNVIVSDIHIIAPDE 190
           SSWWE       RPTA+ F +CN L+L  + H++S   HI I+ CN V +S + I AP+ 
Sbjct: 130 SSWWEH---KGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPES 189

Query: 191 SPNTDGIDISRSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGPGHGISIGSLGEDG 250
           SPNTDGID+  SSNV++Q+ ++ TGDDCIAIN+GT+NI I G+ CGPGHGISIGSLG+DG
Sbjct: 190 SPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDG 249

Query: 251 EYSVVENVYVSDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLESTKNPIIIDQYYTAY 310
           E + VENV V +C  +GT NG RIKTW+GG GYAR ITF  ITL++ +NPIIIDQ+Y   
Sbjct: 250 ETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGG 309

Query: 311 AS--ARKNKAMDIKVSDVTYREVNGTSANENAITLNCS-RARCTNIVMDNVNIRT--STP 350
            S  A+  K+  ++VS V +    GTS +E  +   CS R  CT I + ++ I T  S  
Sbjct: 310 DSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGS 369

[Close]

BLAST of Spg002942 vs. TAIR 10
Match: AT3G15720.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 299.3 bits (765), Expect = 4.1e-81
Identity = 171/389 (43.96%), Postives = 227/389 (58.35%), Query Frame = 0


Query: 11  FSIAILSSWTISASTYFNVLDYGAAGNGKTDDS--------------------------- 70
           FS+  L  +T  +S   +V  +GA G+G TDDS                           
Sbjct: 9   FSLFFLQIFT--SSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDGQFVVPAGMTF 68

Query: 71  ------------------ELRGTLIAPSKGAWPNDKGRWIQFFDIDGLTVKGPGEIDAQG 130
                             ++ G L+APSKG W  DK +WI F DI+GL ++G GEI+ QG
Sbjct: 69  MLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKGDKDQWILFTDIEGLVIEGDGEINGQG 128

Query: 131 SSWWEDCHTHCKRPTAVFFHNCNGLQLREMKHVNSGKNHISINVCNNVIVSDIHIIAPDE 190
           SSWWE       RPT   F +CN L+L  + H++S   HI I+ CN V +S + I AP+ 
Sbjct: 129 SSWWEH---KGSRPT---FRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPES 188

Query: 191 SPNTDGIDISRSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGPGHGISIGSLGEDG 250
           SPNTDGID+  SSNV++Q+ ++ TGDDCIAIN+GT+NI I G+ CGPGHGISIGSLG+DG
Sbjct: 189 SPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDG 248

Query: 251 EYSVVENVYVSDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLESTKNPIIIDQYYTAY 310
           E + VENV V +C  +GT NG RIKTW+GG GYAR ITF  ITL++ +NPIIIDQ+Y   
Sbjct: 249 ETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGG 308

Query: 311 AS--ARKNKAMDIKVSDVTYREVNGTSANENAITLNCS-RARCTNIVMDNVNIRT--STP 350
            S  A+  K+  ++VS V +    GTS +E  +   CS R  CT I + ++ I T  S  
Sbjct: 309 DSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGS 368

[Close]

BLAST of Spg002942 vs. TAIR 10
Match: AT4G35670.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 284.3 bits (726), Expect = 1.4e-76
Identity = 161/381 (42.26%), Postives = 224/381 (58.79%), Query Frame = 0


Query: 10  VFSIAILSSWTISASTY---FNVLDYGAAGNGKTDDSE---------------------- 69
           +  I+IL ++      Y   +NVL++ A G+G+TDDSE                      
Sbjct: 4   ILDISILINFLCFGIVYGKNYNVLNFDAKGDGQTDDSEAFLQAWTAACGGDGDIKTLLIP 63

Query: 70  ------------------------LRGTLIAPS-KGAWPNDKGR-WIQFFDIDGLTVKGP 129
                                   L GT++APS K AW +   R WI+F  + GL + G 
Sbjct: 64  SGKTFLLQPTVFQGPCKSSSIKVQLDGTIVAPSDKFAWSDPISRMWIKFSTVSGLIIVGS 123

Query: 130 GEIDAQGSSWWEDCHTHCKRPTAVFFHNCNGLQLREMKHVNSGKNHISINVCNNVIVSDI 189
           G ID++GSS+WE      +RPTA+    C+ L++  +  ++S KNHISI  CN V +S+I
Sbjct: 124 GTIDSRGSSFWELNLKASQRPTALHISKCDNLRINGITSIDSPKNHISIKTCNTVAISNI 183

Query: 190 HIIAPDESPNTDGIDISRSSNVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGPGHGISI 249
           ++ AP+ SPNTDGIDIS S+N+ + +S ++TGDDCIAIN+G++NI I G+ CGPGHGIS+
Sbjct: 184 NLFAPETSPNTDGIDISDSTNINIFDSTIQTGDDCIAINSGSSNINITGINCGPGHGISV 243

Query: 250 GSLGEDGEYSVVENVYVSDCLLKGTQNGVRIKTWEGGYGYARNITFEKITLESTKNPIII 309
           GSLG  G  + V +V V+ C    T NG RIKTW GG GYARNI+F  ITL +TKNPIII
Sbjct: 244 GSLGAGGAEAKVSDVQVTHCTFNQTTNGARIKTWLGGQGYARNISFTDITLVNTKNPIII 303

Query: 310 DQYYTAYASARKNKAMDIKVSDVTYREVNGTSANENAITLNCSR-ARCTNIVMDNVNIRT 339
           DQ+Y      R  +   + +S+V + +  GTS+N+NAITL CS    C ++VMD ++I  
Sbjct: 304 DQHY--IDKGRLTEESAVAISNVKFVDFRGTSSNKNAITLKCSETTHCVDVVMDGIDITM 363

[Close]

BLAST of Spg002942 vs. TAIR 10
Match: AT5G27530.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 270.4 bits (690), Expect = 2.0e-72
Identity = 152/358 (42.46%), Postives = 206/358 (57.54%), Query Frame = 0


Query: 27  FNVLDYGAAGNGKTDDS------------------------------------------- 86
           +NVL +GA G+G+TDDS                                           
Sbjct: 53  YNVLKFGAEGDGQTDDSNAFLQAWNATCGGEENINTLFIPSGKTYLLQPIEFKGPCKSTS 112

Query: 87  ---ELRGTLIAPSK-GAWPNDKGR-WIQFFDIDGLTVKGPGEIDAQGSSWWEDCHTHCKR 146
              +L G ++APS   +W N K + WI F  + GL + G G I+ +GSS+WE  H     
Sbjct: 113 IKLQLDGIIVAPSNITSWSNPKSQTWISFSGVPGLMIDGSGTINGRGSSFWEALH----- 172

Query: 147 PTAVFFHNCNGLQLREMKHVNSGKNHISINVCNNVIVSDIHIIAPDESPNTDGIDISRSS 206
                  NCN L +  +  ++S K+HISI  C+ V +S I+I+AP+ SPNTDGIDIS S+
Sbjct: 173 -----ICNCNNLTINGITSIDSPKSHISIKNCHYVAISKINILAPENSPNTDGIDISYST 232

Query: 207 NVLVQNSLMETGDDCIAINNGTTNIKIVGVTCGPGHGISIGSLGEDGEYSVVENVYVSDC 266
           NV + +S ++TGDDCIAIN G+++I I  V CGPGHGIS+GSLG DGE + V +VYV+ C
Sbjct: 233 NVNIFDSTIQTGDDCIAINTGSSSINITQVNCGPGHGISVGSLGADGENAAVSDVYVTQC 292

Query: 267 LLKGTQNGVRIKTWEGGYGYARNITFEKITLESTKNPIIIDQYYTAYASARKNKAMDIKV 326
               T NG RIKTW+GG GYARNI+FE ITL + +NPIIIDQ YT        K   + +
Sbjct: 293 TFNKTTNGARIKTWQGGQGYARNISFENITLINVQNPIIIDQQYTDKVLLDATKDSAVAI 352

Query: 327 SDVTYREVNGTSANENAITLNCSR-ARCTNIVMDNVNIRTSTPGKEAKAFCQNVEGKS 336
           S V Y    GT+ NE+AI L CS    C ++V+D++ + T   G++ K  C+NVEG+S
Sbjct: 353 SSVKYVGFQGTTLNEDAIMLKCSAITYCKDMVIDDIEV-TMENGEKPKVECENVEGES 399

[Close]

BLAST of Spg002942 vs. TAIR 10
Match: AT5G17200.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 266.9 bits (681), Expect = 2.2e-71
Identity = 139/324 (42.90%), Postives = 196/324 (60.49%), Query Frame = 0


Query: 45  LRGTLIAPSKGAWPNDKGRWIQFFDIDGLTVKGPGEIDAQGSSWWEDCHTHCKRPTAVFF 104
           +RG L+AP    +      WI F  I+GL V G G ID +GS WW + +    RP A+ F
Sbjct: 102 IRGNLVAPGYTWYAGRYTTWISFDSINGLVVTGGGTIDGRGSLWWGNVN---NRPCAMHF 161

Query: 105 HNCNGLQLREMKHVNSGKNHISINVCNNVIVSDIHIIAPDESPNTDGIDISRSSNVLVQN 164
           +NCNGL++  ++H+NS +NH+ ++   N+ V  + + AP +SPNTDGIDIS    V + +
Sbjct: 162 NNCNGLRISNLRHLNSPRNHVGLSCSQNIEVRGLRMTAPGDSPNTDGIDISNCIGVHIHD 221

Query: 165 SLMETGDDCIAINNGTTNIKIVGVTCGPGHGISIGSLGEDGEYSVVENVYVSDCLLKGTQ 224
           S++ TGDDCIAIN+G+++I I G+ CGPGHGIS+GSLG  G++  VE V V +C    TQ
Sbjct: 222 SVIATGDDCIAINSGSSHINITGIFCGPGHGISVGSLGVTGDFETVEEVRVKNCTFTKTQ 281

Query: 225 NGVRIKTWEGGYGYARNITFEKITLESTKNPIIIDQYY---------------------T 284
           NGVRIKT++ G GYAR I+FE I + +++NPIIIDQ Y                     T
Sbjct: 282 NGVRIKTYQNGSGYARKISFEDINMVASENPIIIDQTYHNGGTNGGISKSSSSYQNCHLT 341

Query: 285 AYASARKNKAMDIKVSDVTYREVNGTSANENAITLNC-SRARCTNIVMDNVNIRTSTPGK 344
           A           +KV+DV Y  + G+SA++  ITLNC +   C++IVMDNVN+ ++T G 
Sbjct: 342 AKQRTPSGNGKGVKVTDVRYARIRGSSASDQDITLNCDADLGCSDIVMDNVNMVSATFGH 401

Query: 345 EAKAFCQNVEGKSKSTIIPTVPCL 347
           +  + C+N  G   S     V CL
Sbjct: 402 KLFSSCKNAHG---SLFASKVDCL 419

The following BLAST results are available for this feature:

BLAST of Spg002942 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888688.1	3.1e-147	67.17	probable polygalacturonase At3g15720 [Benincasa hispida]	[more]
XP_023552838.1	1.3e-145	65.08	probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo]	[more]
XP_038888676.1	6.0e-143	64.57	probable polygalacturonase At3g15720 [Benincasa hispida]	[more]
XP_022141108.1	5.1e-142	63.22	probable polygalacturonase At3g15720 [Momordica charantia]	[more]
XP_022979578.1	5.1e-142	64.82	probable polygalacturonase At3g15720 [Cucurbita maxima]	[more]

BLAST of Spg002942 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LW07	4.7e-82	44.22	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572...	[more]
Q8RY29	1.2e-64	38.62	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2	[more]
Q94AJ5	3.5e-61	38.34	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
Q9SFB7	4.6e-61	40.60	Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2	[more]
O23147	1.3e-60	37.53	Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1	[more]

BLAST of Spg002942 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IWN4	2.5e-142	64.82	probable polygalacturonase At3g15720 OS=Cucurbita maxima OX=3661 GN=LOC111479259...	[more]
A0A6J1CJJ6	2.5e-142	63.22	probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A0A0KZ95	1.3e-140	62.94	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G025750 PE=3 SV=1	[more]
A0A6J1GVS6	8.7e-140	63.82	probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC1114579...	[more]
A0A6J1CIX8	1.3e-138	63.96	probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]

BLAST of Spg002942 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15720.1	3.3e-83	44.22	Pectin lyase-like superfamily protein	[more]
AT3G15720.2	4.1e-81	43.96	Pectin lyase-like superfamily protein	[more]
AT4G35670.1	1.4e-76	42.26	Pectin lyase-like superfamily protein	[more]
AT5G27530.1	2.0e-72	42.46	Pectin lyase-like superfamily protein	[more]
AT5G17200.1	2.2e-71	42.90	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 157..178 e-value: 320.0 score: 10.1 coord: 210..231 e-value: 18.0 score: 14.3 coord: 180..200 e-value: 6900.0 score: 1.3 coord: 130..156 e-value: 62.0 score: 12.5 coord: 239..260 e-value: 1800.0 score: 5.3 coord: 273..300 e-value: 7000.0 score: 1.2
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 14..345 e-value: 2.1E-95 score: 322.1
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 45..333 e-value: 5.3E-84 score: 282.1
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 187..200
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 44..346
None	No IPR available	PANTHER	PTHR31375:SF232	SUBFAMILY NOT NAMED	coord: 44..346
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 12..346

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg002942.1	Spg002942.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity