Spg002787 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg002787
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionsubtilisin-like protease SBT1.3
Locationscaffold6: 2004756 .. 2007074 (-)
RNA-Seq ExpressionSpg002787
SyntenySpg002787
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGATTCTCTTGTGAAATGGCTCTGTTTGCTTCTGTTTCTTCACTGTTTGCTTCTTTCAAGAGCTCAGTTTTTGAAAACTTATGTCGTTCAAATGGACAGGTCTGCAATGCCGGCCTCATTCTCGGATCATTTTCAGTGGTACTCCACAGTTCTAAGCAATGTGGTGGTTAGCCCTGAGAGAGAAGGTAATGGGGGAGGTGAAGAGAGGATCATTTACAGTTACCAGAGTGTTTTCCATGGAGTTGCAGCTCGGTTGAGTGAAGAAGAGGCTGAGAGGCTCGAGGAAGAAGATGGGGTGTTGGCCATTTTTCCGGAGACTAAGTATGAGCTTCATACAACGAGAAGCCCCAAGTTTCTTGGGCTTGAACCGGCTGACAGCAACAGCGCCTGGTCTCAGCAGATTGCAGACCATGACGTGATCGTTGGAGTTTTGGATACTGGGATTTGGCCGGAGAGTGAGAGCTTCAACGACGCTGGAATGTCGCCGGTGCCGCCGCATTGGAAAGGGGAATGTGAAACAGGGCGAGGCTTTACGAAACAGAATTGTAATAGAAAGATCGTCGGCGCCAGAGTGTTCTACCATGGGTATGAAGCTGCGACTGGGAAATTTAACGAACAGTTGGAGTACAAATCGCCGAGGGATCAAGATGGGCATGGAACTCACACGGCGGCCACCGTCGCCGGCTCTCCGGTGGCCGGCGCGAGCCTCCTTGGTTATGCTTATGGAACGGCCAGAGGAATGGCGCCCGGTGCAAGAATTGCCGCCTACAAAGTCTGTTGGGTCGGCGGCTGCTTTAGCTCCGACATTTTGTCGGCTGTGGATAGAGCTGTGGCCGACGGAGTAAATGTTCTGTCCATCTCTTTGGGAGGTGGGGTCTCTTCTTACTATCGCGATAGCCTTTCAGTTGCAGCATTTGGGGCAATGGAGATGGGCGTGTTTGTATCCTGCTCGGCCGGAAATGGAGGGCCGGACCCTGTCAGTCTCACAAACGTATCGCCATGGATAACCACCGTCGGCGCCAGTACAATGGACAGAGATTTCCCGGCCATTGTCAAGCTCGGTGATGGCAGAACAGTCACCGGCGTTTCACTTTACAGAGGAAGAATCACAATTCCGGAAAACAAGCAATTCCCAGTTGTGTACATGGGGAGTAATTCGAGCAGCCCTGATCCGAGCTCGCTCTGTTTGGAAGGAACTTTAGATCCCCATTTCGTGGCCGGAAAAATTGTGATATGCGATCGAGGAATTAGCCCTCGGGTCCAGAAGGGTGTGGTGGTGAAAAATGCAGGTGGGATTGGGATGATTCTGTCTAACACGGCGGCGAATGGGGAAGAACTCGTTGCCGATTGCCATTTGGTACCAGCTGTCGCCGTCGGAGAAAAAGAAGGCAAAGCAATTAAACAATACGCATTAACGAATCGAAAAGCAACGGCGACTCTAGGGTTCTTGGGGACAAGATTGGGGGTTAAACCCTCGCCGGTGGTGGCGGCGTTTTCATCCAGAGGACCAAATTTCCTCACACTCGAAATCCTCAAGCCCGATCTAGTGGCTCCCGGCGTCAACATCCTCGCCGCTTGGACCGGAAAAACAGGGCCGTCGAGCTTGACGACGGACACCAGAAGGGTCAAATTCAACATTCTTTCAGGAACTTCAATGTCGTGCCCTCACGTTAGTGGCGTAGCGGCTCTGATCAAATCAAAGCATCCCGATTGGAGCCCGTCGGCAATCAAATCGGCGCTGATGACCACCGCTTACGTCCACGACAACACCTACAAGCCTCTCAGAGACTCCTCAGCCGCATCGCCGTCGAGTCCGTACGACCATGGCGCCGGCCACATAAACCCAAGAAAAGCCCTAGATCCTGGTTTGGTTTACGAAATTCAGCCGCAAGATTACTTCGAATTCCTCTGTACACAGGATTTAACCCCATCGCAACTCAAAGTCTTCTCCAAGAATTCAAACAGAACGTGCCATCGCCTTCTCCCCAACCCCGGAGATTTGAATTACCCGGCCATCTCCGCCGTTTTCCCAGAGAAAACCTCCGTCACTTCCCTGACCCTTCACAGAACCGTCACCAACGTCGGCCCAGCAGCCTCCAGTTACCGTGCAGTGGTGACGCTGTTCAACGGCGCCGCCGTGAAAGTCGAGCCGGAGAGTCTGAACTTCACGAGGAGATATCAGAAGCTCTCTTACAGAATCACTTTTCTGTCGAAGAAGAGACAGAGTATGCCGGAATTCGGAGGGCTGATTTGGAAGGATGGAAGTCACAAAGTGAGAAGCCCCATTGTCATCACTTGGTTGTCGTTTGTTTGA

mRNA sequence

ATGGCTGATTCTCTTGTGAAATGGCTCTGTTTGCTTCTGTTTCTTCACTGTTTGCTTCTTTCAAGAGCTCAGTTTTTGAAAACTTATGTCGTTCAAATGGACAGGTCTGCAATGCCGGCCTCATTCTCGGATCATTTTCAGTGGTACTCCACAGTTCTAAGCAATGTGGTGGTTAGCCCTGAGAGAGAAGGTAATGGGGGAGGTGAAGAGAGGATCATTTACAGTTACCAGAGTGTTTTCCATGGAGTTGCAGCTCGGTTGAGTGAAGAAGAGGCTGAGAGGCTCGAGGAAGAAGATGGGGTGTTGGCCATTTTTCCGGAGACTAAGTATGAGCTTCATACAACGAGAAGCCCCAAGTTTCTTGGGCTTGAACCGGCTGACAGCAACAGCGCCTGGTCTCAGCAGATTGCAGACCATGACGTGATCGTTGGAGTTTTGGATACTGGGATTTGGCCGGAGAGTGAGAGCTTCAACGACGCTGGAATGTCGCCGGTGCCGCCGCATTGGAAAGGGGAATGTGAAACAGGGCGAGGCTTTACGAAACAGAATTGTAATAGAAAGATCGTCGGCGCCAGAGTGTTCTACCATGGGTATGAAGCTGCGACTGGGAAATTTAACGAACAGTTGGAGTACAAATCGCCGAGGGATCAAGATGGGCATGGAACTCACACGGCGGCCACCGTCGCCGGCTCTCCGGTGGCCGGCGCGAGCCTCCTTGGTTATGCTTATGGAACGGCCAGAGGAATGGCGCCCGGTGCAAGAATTGCCGCCTACAAAGTCTGTTGGGTCGGCGGCTGCTTTAGCTCCGACATTTTGTCGGCTGTGGATAGAGCTGTGGCCGACGGAGTAAATGTTCTGTCCATCTCTTTGGGAGGTGGGGTCTCTTCTTACTATCGCGATAGCCTTTCAGTTGCAGCATTTGGGGCAATGGAGATGGGCGTGTTTGTATCCTGCTCGGCCGGAAATGGAGGGCCGGACCCTGTCAGTCTCACAAACGTATCGCCATGGATAACCACCGTCGGCGCCAGTACAATGGACAGAGATTTCCCGGCCATTGTCAAGCTCGGTGATGGCAGAACAGTCACCGGCGTTTCACTTTACAGAGGAAGAATCACAATTCCGGAAAACAAGCAATTCCCAGTTGTGTACATGGGGAGTAATTCGAGCAGCCCTGATCCGAGCTCGCTCTGTTTGGAAGGAACTTTAGATCCCCATTTCGTGGCCGGAAAAATTGTGATATGCGATCGAGGAATTAGCCCTCGGGTCCAGAAGGGTGTGGTGGTGAAAAATGCAGGTGGGATTGGGATGATTCTGTCTAACACGGCGGCGAATGGGGAAGAACTCGTTGCCGATTGCCATTTGGTACCAGCTGTCGCCGTCGGAGAAAAAGAAGGCAAAGCAATTAAACAATACGCATTAACGAATCGAAAAGCAACGGCGACTCTAGGGTTCTTGGGGACAAGATTGGGGGTTAAACCCTCGCCGGTGGTGGCGGCGTTTTCATCCAGAGGACCAAATTTCCTCACACTCGAAATCCTCAAGCCCGATCTAGTGGCTCCCGGCGTCAACATCCTCGCCGCTTGGACCGGAAAAACAGGGCCGTCGAGCTTGACGACGGACACCAGAAGGGTCAAATTCAACATTCTTTCAGGAACTTCAATGTCGTGCCCTCACGTTAGTGGCGTAGCGGCTCTGATCAAATCAAAGCATCCCGATTGGAGCCCGTCGGCAATCAAATCGGCGCTGATGACCACCGCTTACGTCCACGACAACACCTACAAGCCTCTCAGAGACTCCTCAGCCGCATCGCCGTCGAGTCCGTACGACCATGGCGCCGGCCACATAAACCCAAGAAAAGCCCTAGATCCTGGTTTGGTTTACGAAATTCAGCCGCAAGATTACTTCGAATTCCTCTGTACACAGGATTTAACCCCATCGCAACTCAAAGTCTTCTCCAAGAATTCAAACAGAACGTGCCATCGCCTTCTCCCCAACCCCGGAGATTTGAATTACCCGGCCATCTCCGCCGTTTTCCCAGAGAAAACCTCCGTCACTTCCCTGACCCTTCACAGAACCGTCACCAACGTCGGCCCAGCAGCCTCCAGTTACCGTGCAGTGGTGACGCTGTTCAACGGCGCCGCCGTGAAAGTCGAGCCGGAGAGTCTGAACTTCACGAGGAGATATCAGAAGCTCTCTTACAGAATCACTTTTCTGTCGAAGAAGAGACAGAGTATGCCGGAATTCGGAGGGCTGATTTGGAAGGATGGAAGTCACAAAGTGAGAAGCCCCATTGTCATCACTTGGTTGTCGTTTGTTTGA

Coding sequence (CDS)

ATGGCTGATTCTCTTGTGAAATGGCTCTGTTTGCTTCTGTTTCTTCACTGTTTGCTTCTTTCAAGAGCTCAGTTTTTGAAAACTTATGTCGTTCAAATGGACAGGTCTGCAATGCCGGCCTCATTCTCGGATCATTTTCAGTGGTACTCCACAGTTCTAAGCAATGTGGTGGTTAGCCCTGAGAGAGAAGGTAATGGGGGAGGTGAAGAGAGGATCATTTACAGTTACCAGAGTGTTTTCCATGGAGTTGCAGCTCGGTTGAGTGAAGAAGAGGCTGAGAGGCTCGAGGAAGAAGATGGGGTGTTGGCCATTTTTCCGGAGACTAAGTATGAGCTTCATACAACGAGAAGCCCCAAGTTTCTTGGGCTTGAACCGGCTGACAGCAACAGCGCCTGGTCTCAGCAGATTGCAGACCATGACGTGATCGTTGGAGTTTTGGATACTGGGATTTGGCCGGAGAGTGAGAGCTTCAACGACGCTGGAATGTCGCCGGTGCCGCCGCATTGGAAAGGGGAATGTGAAACAGGGCGAGGCTTTACGAAACAGAATTGTAATAGAAAGATCGTCGGCGCCAGAGTGTTCTACCATGGGTATGAAGCTGCGACTGGGAAATTTAACGAACAGTTGGAGTACAAATCGCCGAGGGATCAAGATGGGCATGGAACTCACACGGCGGCCACCGTCGCCGGCTCTCCGGTGGCCGGCGCGAGCCTCCTTGGTTATGCTTATGGAACGGCCAGAGGAATGGCGCCCGGTGCAAGAATTGCCGCCTACAAAGTCTGTTGGGTCGGCGGCTGCTTTAGCTCCGACATTTTGTCGGCTGTGGATAGAGCTGTGGCCGACGGAGTAAATGTTCTGTCCATCTCTTTGGGAGGTGGGGTCTCTTCTTACTATCGCGATAGCCTTTCAGTTGCAGCATTTGGGGCAATGGAGATGGGCGTGTTTGTATCCTGCTCGGCCGGAAATGGAGGGCCGGACCCTGTCAGTCTCACAAACGTATCGCCATGGATAACCACCGTCGGCGCCAGTACAATGGACAGAGATTTCCCGGCCATTGTCAAGCTCGGTGATGGCAGAACAGTCACCGGCGTTTCACTTTACAGAGGAAGAATCACAATTCCGGAAAACAAGCAATTCCCAGTTGTGTACATGGGGAGTAATTCGAGCAGCCCTGATCCGAGCTCGCTCTGTTTGGAAGGAACTTTAGATCCCCATTTCGTGGCCGGAAAAATTGTGATATGCGATCGAGGAATTAGCCCTCGGGTCCAGAAGGGTGTGGTGGTGAAAAATGCAGGTGGGATTGGGATGATTCTGTCTAACACGGCGGCGAATGGGGAAGAACTCGTTGCCGATTGCCATTTGGTACCAGCTGTCGCCGTCGGAGAAAAAGAAGGCAAAGCAATTAAACAATACGCATTAACGAATCGAAAAGCAACGGCGACTCTAGGGTTCTTGGGGACAAGATTGGGGGTTAAACCCTCGCCGGTGGTGGCGGCGTTTTCATCCAGAGGACCAAATTTCCTCACACTCGAAATCCTCAAGCCCGATCTAGTGGCTCCCGGCGTCAACATCCTCGCCGCTTGGACCGGAAAAACAGGGCCGTCGAGCTTGACGACGGACACCAGAAGGGTCAAATTCAACATTCTTTCAGGAACTTCAATGTCGTGCCCTCACGTTAGTGGCGTAGCGGCTCTGATCAAATCAAAGCATCCCGATTGGAGCCCGTCGGCAATCAAATCGGCGCTGATGACCACCGCTTACGTCCACGACAACACCTACAAGCCTCTCAGAGACTCCTCAGCCGCATCGCCGTCGAGTCCGTACGACCATGGCGCCGGCCACATAAACCCAAGAAAAGCCCTAGATCCTGGTTTGGTTTACGAAATTCAGCCGCAAGATTACTTCGAATTCCTCTGTACACAGGATTTAACCCCATCGCAACTCAAAGTCTTCTCCAAGAATTCAAACAGAACGTGCCATCGCCTTCTCCCCAACCCCGGAGATTTGAATTACCCGGCCATCTCCGCCGTTTTCCCAGAGAAAACCTCCGTCACTTCCCTGACCCTTCACAGAACCGTCACCAACGTCGGCCCAGCAGCCTCCAGTTACCGTGCAGTGGTGACGCTGTTCAACGGCGCCGCCGTGAAAGTCGAGCCGGAGAGTCTGAACTTCACGAGGAGATATCAGAAGCTCTCTTACAGAATCACTTTTCTGTCGAAGAAGAGACAGAGTATGCCGGAATTCGGAGGGCTGATTTGGAAGGATGGAAGTCACAAAGTGAGAAGCCCCATTGTCATCACTTGGTTGTCGTTTGTTTGA

Protein sequence

MADSLVKWLCLLLFLHCLLLSRAQFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPEREGNGGGEERIIYSYQSVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTCHRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEPESLNFTRRYQKLSYRITFLSKKRQSMPEFGGLIWKDGSHKVRSPIVITWLSFV
Homology
[Close]
BLAST of Spg002787 vs. NCBI nr
Match: KAG6576764.1 (Subtilisin-like protease 1.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1467.2 bits (3797), Expect = 0.0e+00
Identity = 723/772 (93.65%), Postives = 749/772 (97.02%), Query Frame = 0 

Query: 1   MADSLVKWLCLLLFLHCLLLSRAQFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           M  + +KWLCLLL   CLLLS AQF+KTYVVQMDRSAMP SFSDH QWYSTVL++VVV+P
Sbjct: 1   MGHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEERIIYSYQSVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPKF 120
           EREGNGGGE+RIIYSYQ+VFHGVAARLSE+EAERLEEEDGVLAIFPETKYELHTTRSP+F
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEDEAERLEEEDGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVP HWKGECETGR FT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           KQNCNRKIVGAR+FYHGYEAATGKFNE+LEYKSPRDQDGHGTHTAATVAGSP AGA+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRT 360
           SLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+GRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLY+GRITIPENKQFPVVYMGSNSS+PDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKATA 480
           RVQKGVVVKNAGG+GMIL+NTAANGEELVADCHLVPAVAVGE+EGKAIK YALTNRK TA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA 480

Query: 481 TLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPL+DSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 AASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTCH 660
            ASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSK SNR+CH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEPE 720
            LLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPA SSY AVVT FNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYRITFLSKKRQSMPEFGGLIWKDGSHKVRSPIVITWLSFV 773
           SLNFTRRYQKLSY+ITFL+KKRQSMPEFGGLIWKDG+H VRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

[Close]
BLAST of Spg002787 vs. NCBI nr
Match: XP_022922929.1 (subtilisin-like protease SBT1.3 [Cucurbita moschata])

HSP 1 Score: 1466.8 bits (3796), Expect = 0.0e+00
Identity = 724/772 (93.78%), Postives = 750/772 (97.15%), Query Frame = 0 

Query: 1   MADSLVKWLCLLLFLHCLLLSRAQFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           MA + +KWLCLLL   CLLLS AQF+KTYVVQMDRSAMP SFSDH QWYSTVL++VVV+P
Sbjct: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEERIIYSYQSVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPKF 120
           EREGNGGGE+RIIYSYQ+VFHGVAARL+EEEAERLEEE+GVLAIFPETKYELHTTRSP+F
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVP HWKGECETGR FT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           KQNCNRKIVGAR+FYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSP AGA+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRT 360
           SLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+GRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLY+GRITIPE+KQFPVVYMGSNSS+PDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKATA 480
           RVQKGVVVKNAGG+GMIL+NTAANGEELVADCHLVPAVAVGE+EGKAIK YALTNRK TA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTA 480

Query: 481 TLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPL+DSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 AASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTCH 660
            ASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSK SNR+CH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEPE 720
            LLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPA SSY AVVT FNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYRITFLSKKRQSMPEFGGLIWKDGSHKVRSPIVITWLSFV 773
           SLNFTRRYQKLSY+ITFL+KKRQSMPEFGGLIWKDG+H VRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

[Close]
BLAST of Spg002787 vs. NCBI nr
Match: XP_023552616.1 (subtilisin-like protease SBT1.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1466.8 bits (3796), Expect = 0.0e+00
Identity = 724/772 (93.78%), Postives = 748/772 (96.89%), Query Frame = 0 

Query: 1   MADSLVKWLCLLLFLHCLLLSRAQFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           MA + +KWLCLLL   CLLLS AQF+KTYVVQMDRSAMP SFSDH QWYSTVL++VVV+P
Sbjct: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEERIIYSYQSVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPKF 120
           EREGNGGGE+RIIYSYQ+VFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSP+F
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVP HWKGECETGR F+
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFS 180

Query: 181 KQNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           KQNCNRKIVGAR+FYHGYEAATGKFNE LEYKSPRDQDGHGTHTAATVAGSP AGA+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRT 360
           SLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+GRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLY+GRITIPENKQFPVVYMGSNSS+PDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKATA 480
           RVQKGVVVKNAGG+GMIL+NTAANGEELVADCHLVPAVAVGE+EGKAIK YALTNRK TA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA 480

Query: 481 TLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPL+DSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 AASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTCH 660
            ASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSK SNR+CH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEPE 720
            LLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPA SSY A VT FNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYRITFLSKKRQSMPEFGGLIWKDGSHKVRSPIVITWLSFV 773
           SLNFTRRYQKLSY+ITFL+KKRQSMPEFGGLIWKDG+H VRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

[Close]
BLAST of Spg002787 vs. NCBI nr
Match: KAA0033586.1 (subtilisin-like protease SBT1.3 [Cucumis melo var. makuwa] >TYK22252.1 subtilisin-like protease SBT1.3 [Cucumis melo var. makuwa])

HSP 1 Score: 1464.1 bits (3789), Expect = 0.0e+00
Identity = 728/773 (94.18%), Postives = 751/773 (97.15%), Query Frame = 0 

Query: 1   MADSLVKWLCLLLFLHCLLLSRAQFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           MADS +K LCLLLFL  LLLS A FLKTYVVQMDRSAMPASF++HF+WYS VLSNVVV  
Sbjct: 1   MADSPIKCLCLLLFLDSLLLSSALFLKTYVVQMDRSAMPASFTNHFEWYSNVLSNVVVDL 60

Query: 61  EREGN-GGGEERIIYSYQSVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPK 120
           EREGN GGGEERIIYSYQ+VFHGVAARLSEEE E+LEEEDGV+AIFPE KYELHTTRSP+
Sbjct: 61  EREGNGGGGEERIIYSYQNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPR 120

Query: 121 FLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGF 180
           FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPES+SF+DAGMSPVP HWKGECETGRGF
Sbjct: 121 FLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGF 180

Query: 181 TKQNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240
           TKQNCNRKIVGARVFYHGY+AATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL
Sbjct: 181 TKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240

Query: 241 GYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300
           GYAYGTARGMAPGARIAAYKVCW+GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR
Sbjct: 241 GYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300

Query: 301 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 360
           DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR
Sbjct: 301 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 360

Query: 361 TVTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGIS 420
           T++G SLYRGRITIPENKQFP+VYMGSNSSSPDPSSLCLEGTLDPH VAGKIVICDRGIS
Sbjct: 361 TMSGASLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHVVAGKIVICDRGIS 420

Query: 421 PRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKAT 480
           PRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKAT
Sbjct: 421 PRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKAT 480

Query: 481 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 540
           ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT
Sbjct: 481 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 540

Query: 541 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDS 600
           DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAY+HDNTYKPL+DS
Sbjct: 541 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYIHDNTYKPLQDS 600

Query: 601 SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTC 660
           SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYF+FLCTQDLTP+QLKVFSK SNRTC
Sbjct: 601 SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTC 660

Query: 661 HRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEP 720
            RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPA SSY AVVT F GAAVKVEP
Sbjct: 661 RRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFKGAAVKVEP 720

Query: 721 ESLNFTRRYQKLSYRITFLSKKRQSMPEFGGLIWKDGSHKVRSPIVITWLSFV 773
           ESLNFTRRY+KLSYRITF++KKR SMPEFGGLIWKDGSHKVRSPIVITWLSFV
Sbjct: 721 ESLNFTRRYEKLSYRITFVTKKRLSMPEFGGLIWKDGSHKVRSPIVITWLSFV 773

[Close]
BLAST of Spg002787 vs. NCBI nr
Match: XP_022984431.1 (subtilisin-like protease SBT1.3 [Cucurbita maxima])

HSP 1 Score: 1459.5 bits (3777), Expect = 0.0e+00
Identity = 720/772 (93.26%), Postives = 746/772 (96.63%), Query Frame = 0 

Query: 1   MADSLVKWLCLLLFLHCLLLSRAQFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           MA + +KWLCLLL   C LLS AQFLKTYVVQMDRSAMP SFSDH QWYSTVL++VVV+P
Sbjct: 1   MAHTPLKWLCLLLVFDCSLLSSAQFLKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEERIIYSYQSVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPKF 120
           EREGNGGGE+RIIYSYQ+VFHGVAARLSEEEAERLEEEDGVLAIFPETKY+LHTTRSP+F
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYDLHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVP HWKGECETGR FT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           KQNCNRKIVGAR+FYHGYEAATGKFNE+LEYKSPRDQDGHGTHTAATVAGSP A A+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTASANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRT 360
           SLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+GRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLY+GRITIPENKQFPVVYMGSNSS+PDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKATA 480
           RVQKGVVVKNAGG+GMIL+NTAANGEELVADCHLVPAVAVGE+EGKAIK YALTNRKATA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKATA 480

Query: 481 TLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           +LGFLGT+LGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 SLGFLGTKLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPL+DSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 AASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTCH 660
             SPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSK SNR+CH
Sbjct: 601 GTSPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEPE 720
            LLPNPGDLNYPAISAVFPEK SVTSLTLHRTVTNVGPA SSY AV T FNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKASVTSLTLHRTVTNVGPATSSYHAVATPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYRITFLSKKRQSMPEFGGLIWKDGSHKVRSPIVITWLSFV 773
           SLNFTRRYQKLSY+ITFL+KKRQSMPEFGGLIWKDG+H VRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

[Close]
BLAST of Spg002787 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 1161.7 bits (3004), Expect = 0.0e+00
Identity = 560/761 (73.59%), Postives = 658/761 (86.47%), Query Frame = 0 

Query: 12  LLFLHCLLLSRAQFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVV--VSPEREGNGGGE 71
           L+FL     ++    KTYV+ MD+SAMP  +++H QWYS+ +++V    S E EGN    
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGN---N 79

Query: 72  ERIIYSYQSVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSN 131
            RI+Y+YQ+ FHG+AA+L++EEAERLEEEDGV+A+ PET+YELHTTRSP FLGLE  +S 
Sbjct: 80  NRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESE 139

Query: 132 SAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNRKIV 191
             W++++ DHDV+VGVLDTGIWPESESFND GMSPVP  W+G CETG+ F K+NCNRKIV
Sbjct: 140 RVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIV 199

Query: 192 GARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGM 251
           GARVFY GYEAATGK +E+LEYKSPRD+DGHGTHTAATVAGSPV GA+L G+AYGTARGM
Sbjct: 200 GARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGM 259

Query: 252 APGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGA 311
           A  AR+AAYKVCWVGGCFSSDILSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FGA
Sbjct: 260 AQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGA 319

Query: 312 MEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRG 371
           MEMGVFVSCSAGNGGPDP+SLTNVSPWITTVGASTMDRDFPA VK+G  RT  GVSLY+G
Sbjct: 320 MEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKG 379

Query: 372 RITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVK 431
           R  +P+NKQ+P+VY+G N+SSPDP+S CL+G LD   VAGKIVICDRG++PRVQKG VVK
Sbjct: 380 RTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVK 439

Query: 432 NAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKATATLGFLGTRL 491
            AGGIGM+L+NTA NGEELVAD H++PAVAVGEKEGK IKQYA+T++KATA+L  LGTR+
Sbjct: 440 RAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRI 499

Query: 492 GVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNIL 551
           G+KPSPVVAAFSSRGPNFL+LEILKPDL+APGVNILAAWTG   PSSL++D RRVKFNIL
Sbjct: 500 GIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNIL 559

Query: 552 SGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDSSAASPSSPYD 611
           SGTSMSCPHVSGVAALIKS+HPDWSP+AIKSALMTTAYVHDN +KPL D+S A+PSSPYD
Sbjct: 560 SGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYD 619

Query: 612 HGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTC-HRLLPNPGD 671
           HGAGHI+P +A DPGLVY+I PQ+YFEFLCTQDL+PSQLKVF+K+SNRTC H L  NPG+
Sbjct: 620 HGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGN 679

Query: 672 LNYPAISAVFPEKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEPESLNFTRRY 731
           LNYPAISA+FPE T V ++TL RTVTNVGP  SSY+  V+ F GA+V V+P++LNFT ++
Sbjct: 680 LNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKH 739

Query: 732 QKLSYRITFLSKKRQSMPEFGGLIWKDGSHKVRSPIVITWL 770
           QKLSY +TF ++ R   PEFGGL+WK  +HKVRSP++ITWL
Sbjct: 740 QKLSYTVTFRTRFRMKRPEFGGLVWKSTTHKVRSPVIITWL 777

[Close]
BLAST of Spg002787 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 803.1 bits (2073), Expect = 2.7e-231
Identity = 412/768 (53.65%), Postives = 531/768 (69.14%), Query Frame = 0 

Query: 4   SLVKWLCLLLFLHCLLLSRAQFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPERE 63
           S   +  LL    C + S +    TY+V M +S MP+SF  H  WY + L ++  S E  
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE-- 66

Query: 64  GNGGGEERIIYSYQSVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPKFLGL 123
                   ++Y+Y++  HG + RL++EEA+ L  + GV+++ PE +YELHTTR+P FLGL
Sbjct: 67  --------LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGL 126

Query: 124 EPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQN 183
           +   ++  + +  +  DV+VGVLDTG+WPES+S++D G  P+P  WKG CE G  FT   
Sbjct: 127 DEHTAD-LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASL 186

Query: 184 CNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAY 243
           CNRK++GAR F  GYE+  G  +E  E +SPRD DGHGTHT++T AGS V GASLLGYA 
Sbjct: 187 CNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYAS 246

Query: 244 GTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLS 303
           GTARGMAP AR+A YKVCW+GGCFSSDIL+A+D+A+AD VNVLS+SLGGG+S YYRD ++
Sbjct: 247 GTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVA 306

Query: 304 VAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTG 363
           + AF AME G+ VSCSAGN GP   SL+NV+PWITTVGA T+DRDFPA+  LG+G+  TG
Sbjct: 307 IGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTG 366

Query: 364 VSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQ 423
           VSL++G   +P+ K  P +Y G N+S+    +LC+ GTL P  V GKIV+CDRGI+ RVQ
Sbjct: 367 VSLFKGE-ALPD-KLLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQ 426

Query: 424 KGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKATATLG 483
           KG VVK AGG+GMIL+NTAANGEELVAD HL+PA  VGEK G  I+ Y  T+   TA++ 
Sbjct: 427 KGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASIS 486

Query: 484 FLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRR 543
            LGT +GVKPSPVVAAFSSRGPN +T  ILKPDL+APGVNILAAWTG  GP+ L +D+RR
Sbjct: 487 ILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRR 546

Query: 544 VKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDSSAAS 603
           V+FNI+SGTSMSCPHVSG+AAL+KS HP+WSP+AI+SALMTTAY      KPL D +   
Sbjct: 547 VEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGK 606

Query: 604 PSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTCHRLL 663
           PS+P+DHGAGH++P  A +PGL+Y++  +DY  FLC  + T  Q++  S+  N TC    
Sbjct: 607 PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRR-NYTCDPSK 666

Query: 664 P-NPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEPESL 723
             +  DLNYP+ +        V +    RTVT+VG A +    V +   G  + VEP  L
Sbjct: 667 SYSVADLNYPSFAV---NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVL 726

Query: 724 NFTRRYQKLSYRITFL--SKKRQSMPEFGGLIWKDGSHKVRSPIVITW 769
           NF    +K SY +TF   S K      FG + W DG H V SP+ I+W
Sbjct: 727 NFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

[Close]
BLAST of Spg002787 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 9.6e-221
Identity = 396/763 (51.90%), Postives = 517/763 (67.76%), Query Frame = 0 

Query: 14  FLHCLLLSRAQFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPEREGNGGGEERII 73
           FL  LL + A+  KTY+++++ S  P SF  H  WY++ L++             E  ++
Sbjct: 17  FLFLLLHTTAK--KTYIIRVNHSDKPESFLTHHDWYTSQLNS-------------ESSLL 76

Query: 74  YSYQSVFHGVAARLSEEEAER-LEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNSAW 133
           Y+Y + FHG +A L   EA+  L   + +L IF +  Y LHTTR+P+FLGL         
Sbjct: 77  YTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN--SEFGVH 136

Query: 134 SQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNRKIVGAR 193
               + + VI+GVLDTG+WPES SF+D  M  +P  WKGECE+G  F  + CN+K++GAR
Sbjct: 137 DLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGAR 196

Query: 194 VFYHGYEAAT-GKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAP 253
            F  G++ A+ G F+ + E  SPRD DGHGTHT+ T AGS V  AS LGYA GTARGMA 
Sbjct: 197 SFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMAT 256

Query: 254 GARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAME 313
            AR+A YKVCW  GCF SDIL+A+DRA+ DGV+VLS+SLGGG + YYRD++++ AF AME
Sbjct: 257 RARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAME 316

Query: 314 MGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGRI 373
            GVFVSCSAGN GP   S+ NV+PW+ TVGA T+DRDFPA   LG+G+ +TGVSLY G  
Sbjct: 317 RGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG-- 376

Query: 374 TIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNA 433
                K   +VY   NSSS   S+LCL G+LD   V GKIV+CDRG++ RV+KG VV++A
Sbjct: 377 VGMGTKPLELVYNKGNSSS---SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDA 436

Query: 434 GGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKATATLGFLGTRLGV 493
           GG+GMI++NTAA+GEELVAD HL+PA+AVG+K G  +++Y  ++ K TA L F GT L V
Sbjct: 437 GGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDV 496

Query: 494 KPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSG 553
           KPSPVVAAFSSRGPN +T EILKPD++ PGVNILA W+   GP+ L  D+RR +FNI+SG
Sbjct: 497 KPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSG 556

Query: 554 TSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDSSAASPSSPYDHG 613
           TSMSCPH+SG+A L+K+ HP+WSPSAIKSALMTTAYV DNT  PL D++  S S+PY HG
Sbjct: 557 TSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHG 616

Query: 614 AGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTCHRLLPNPGDLNY 673
           +GH++P+KAL PGLVY+I  ++Y  FLC+ D T   +    K  +  C +   +PG LNY
Sbjct: 617 SGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNY 676

Query: 674 PAISAVFPEKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEPESLNFTRRYQKL 733
           P+ S +F  K  V      R VTNVG A+S Y+  V       + V+P  L+F    +K 
Sbjct: 677 PSFSVLFGGKRVV---RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKK 736

Query: 734 SYRITFLSKKRQSM---PEFGGLIWKDGSHKVRSPIVITWLSF 772
            Y +TF+SKK  SM    EFG + W +  H+VRSP+  +W  F
Sbjct: 737 RYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNRF 754

[Close]
BLAST of Spg002787 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 751.5 bits (1939), Expect = 9.3e-216
Identity = 381/754 (50.53%), Postives = 516/754 (68.44%), Query Frame = 0 

Query: 28  TYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPEREGNGGGEERIIYSYQSVFHGVAARL 87
           TY+V +D  A P+ F  HF WY++ L+++  SP           II++Y +VFHG +ARL
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP---------PSIIHTYDTVFHGFSARL 86

Query: 88  SEEEAERLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNSAWSQQIADHDVIVGVLD 147
           + ++A +L +   V+++ PE    LHTTRSP+FLGL   D      +     D+++GV+D
Sbjct: 87  TSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVID 146

Query: 148 TGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNRKIVGARVFYHGYEAATGKFNE 207
           TG+WPE  SF+D G+ PVP  WKG+C   + F +  CNRK+VGAR F  GYEA  GK NE
Sbjct: 147 TGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNE 206

Query: 208 QLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWVGGCF 267
             E++SPRD DGHGTHTA+  AG  V  AS LGYA+G A GMAP AR+AAYKVCW  GC+
Sbjct: 207 TTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCY 266

Query: 268 SSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDP 327
            SDIL+A D AVADGV+V+S+S+GG V  YY D++++ AFGA++ G+FVS SAGNGGP  
Sbjct: 267 DSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGA 326

Query: 328 VSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGRITIPENKQFPVVYMGSN 387
           +++TNV+PW+TTVGA T+DRDFPA VKLG+G+ ++GVS+Y G    P  + +P+VY GS 
Sbjct: 327 LTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDP-GRMYPLVYGGSL 386

Query: 388 SSSPD-PSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGE 447
                  SSLCLEG+LDP+ V GKIV+CDRGI+ R  KG +V+  GG+GMI++N   +GE
Sbjct: 387 LGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGE 446

Query: 448 ELVADCHLVPAVAVGEKEGKAIKQYALTNRKA------TATLGFLGTRLGVKPSPVVAAF 507
            LVADCH++PA +VG   G  I++Y   + K+      TAT+ F GTRLG++P+PVVA+F
Sbjct: 447 GLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASF 506

Query: 508 SSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVS 567
           S+RGPN  T EILKPD++APG+NILAAW  + GPS +T+D RR +FNILSGTSM+CPHVS
Sbjct: 507 SARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVS 566

Query: 568 GVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDSSAASPSSPYDHGAGHINPRKA 627
           G+AAL+K+ HPDWSP+AI+SAL+TTAY  DN+ +P+ D S  + SS  D+G+GH++P KA
Sbjct: 567 GLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKA 626

Query: 628 LDPGLVYEIQPQDYFEFLCTQDLTPSQL-KVFSKNSNRTCHRLLPNPGDLNYPAISAVFP 687
           +DPGLVY+I   DY  FLC  + T + +  +  + ++    R   + G+LNYP+ S VF 
Sbjct: 627 MDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQ 686

Query: 688 E-KTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEPESLNFTRRYQKLSYRITFL 747
           +   S  S    RTVTNVG + S Y   +    G  V VEPE L+F R  QKLS+ +   
Sbjct: 687 QYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVK 746

Query: 748 SKKRQSMP-----EFGGLIWKDGSHKVRSPIVIT 768
           + + +  P     E G ++W DG   V SP+V+T
Sbjct: 747 TTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

[Close]
BLAST of Spg002787 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 3.5e-215
Identity = 396/774 (51.16%), Postives = 522/774 (67.44%), Query Frame = 0 

Query: 9   LCLLLFLHCLLLSRA--QFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPEREGNG 68
           + LLLFL    +S A  Q  KT++ ++D  +MP+ F  H+ WYST  +            
Sbjct: 6   IVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAE----------- 65

Query: 69  GGEERIIYSYQSVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPKFLGLEPA 128
             E RI++ Y +VFHG +A ++ +EA+ L     VLA+F + + ELHTTRSP+FLGL+  
Sbjct: 66  --ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ-- 125

Query: 129 DSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNR 188
           +    WS+     DVI+GV DTGIWPE  SF+D  + P+P  W+G CE+G  F+ +NCNR
Sbjct: 126 NQKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNR 185

Query: 189 KIVGARVFYHGYEAAT-GKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGT 248
           KI+GAR F  G +AA  G  N+ +E+ SPRD DGHGTHT++T AG     AS+ GYA G 
Sbjct: 186 KIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGV 245

Query: 249 ARGMAPGARIAAYKVCWV-GGCFSSDILSAVDRAVADGVNVLSISLGGG---VSSYYRDS 308
           A+G+AP ARIAAYKVCW   GC  SDIL+A D AV DGV+V+SIS+GGG    S YY D 
Sbjct: 246 AKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDP 305

Query: 309 LSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTV 368
           +++ ++GA   G+FVS SAGN GP+ +S+TN++PW+TTVGAST+DR+FPA   LGDG  +
Sbjct: 306 IAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRL 365

Query: 369 TGVSLYRGRITIPEN-KQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISP 428
            GVSLY G   +P N + FPVVY G +  S   +SLC+E TLDP  V GKIVICDRG SP
Sbjct: 366 RGVSLYAG---VPLNGRMFPVVYPGKSGMS--SASLCMENTLDPKQVRGKIVICDRGSSP 425

Query: 429 RVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKATA 488
           RV KG+VVK AGG+GMIL+N A+NGE LV D HL+PA AVG  EG  IK YA ++    A
Sbjct: 426 RVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIA 485

Query: 489 TLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 548
           ++ F GT +G+KP+PV+A+FS RGPN L+ EILKPDL+APGVNILAAWT   GP+ L +D
Sbjct: 486 SIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSD 545

Query: 549 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDSS 608
            R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSP+ I+SA+MTT  + DN+ + L D S
Sbjct: 546 PRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDES 605

Query: 609 AASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTCH 668
               ++PYD+G+GH+N  +A++PGLVY+I   DY  FLC+    P  ++V ++   R   
Sbjct: 606 TGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPT 665

Query: 669 RLLPNPGDLNYPAISAVFP-EKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEP 728
              P+PG+LNYP+I+AVFP  +  + S T+ RT TNVG A + YRA +    G  V V+P
Sbjct: 666 TRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKP 725

Query: 729 ESLNFTRRYQKLSYRITFLSKKR-----QSMPEFGGLIWKD-GSHKVRSPIVIT 768
             L FT   ++ SY +T     R     ++   FG + W D G H VRSPIV+T
Sbjct: 726 PRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

[Close]
BLAST of Spg002787 vs. ExPASy TrEMBL
Match: A0A6J1E4V2 (subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111430759 PE=3 SV=1)

HSP 1 Score: 1466.8 bits (3796), Expect = 0.0e+00
Identity = 724/772 (93.78%), Postives = 750/772 (97.15%), Query Frame = 0 

Query: 1   MADSLVKWLCLLLFLHCLLLSRAQFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           MA + +KWLCLLL   CLLLS AQF+KTYVVQMDRSAMP SFSDH QWYSTVL++VVV+P
Sbjct: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEERIIYSYQSVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPKF 120
           EREGNGGGE+RIIYSYQ+VFHGVAARL+EEEAERLEEE+GVLAIFPETKYELHTTRSP+F
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVP HWKGECETGR FT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           KQNCNRKIVGAR+FYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSP AGA+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRT 360
           SLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+GRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLY+GRITIPE+KQFPVVYMGSNSS+PDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKATA 480
           RVQKGVVVKNAGG+GMIL+NTAANGEELVADCHLVPAVAVGE+EGKAIK YALTNRK TA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTA 480

Query: 481 TLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPL+DSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 AASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTCH 660
            ASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSK SNR+CH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEPE 720
            LLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPA SSY AVVT FNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYRITFLSKKRQSMPEFGGLIWKDGSHKVRSPIVITWLSFV 773
           SLNFTRRYQKLSY+ITFL+KKRQSMPEFGGLIWKDG+H VRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

[Close]
BLAST of Spg002787 vs. ExPASy TrEMBL
Match: A0A5A7SUB4 (Subtilisin-like protease SBT1.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold440G00040 PE=3 SV=1)

HSP 1 Score: 1464.1 bits (3789), Expect = 0.0e+00
Identity = 728/773 (94.18%), Postives = 751/773 (97.15%), Query Frame = 0 

Query: 1   MADSLVKWLCLLLFLHCLLLSRAQFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           MADS +K LCLLLFL  LLLS A FLKTYVVQMDRSAMPASF++HF+WYS VLSNVVV  
Sbjct: 1   MADSPIKCLCLLLFLDSLLLSSALFLKTYVVQMDRSAMPASFTNHFEWYSNVLSNVVVDL 60

Query: 61  EREGN-GGGEERIIYSYQSVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPK 120
           EREGN GGGEERIIYSYQ+VFHGVAARLSEEE E+LEEEDGV+AIFPE KYELHTTRSP+
Sbjct: 61  EREGNGGGGEERIIYSYQNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPR 120

Query: 121 FLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGF 180
           FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPES+SF+DAGMSPVP HWKGECETGRGF
Sbjct: 121 FLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGF 180

Query: 181 TKQNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240
           TKQNCNRKIVGARVFYHGY+AATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL
Sbjct: 181 TKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240

Query: 241 GYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300
           GYAYGTARGMAPGARIAAYKVCW+GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR
Sbjct: 241 GYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300

Query: 301 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 360
           DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR
Sbjct: 301 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 360

Query: 361 TVTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGIS 420
           T++G SLYRGRITIPENKQFP+VYMGSNSSSPDPSSLCLEGTLDPH VAGKIVICDRGIS
Sbjct: 361 TMSGASLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHVVAGKIVICDRGIS 420

Query: 421 PRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKAT 480
           PRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKAT
Sbjct: 421 PRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKAT 480

Query: 481 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 540
           ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT
Sbjct: 481 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 540

Query: 541 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDS 600
           DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAY+HDNTYKPL+DS
Sbjct: 541 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYIHDNTYKPLQDS 600

Query: 601 SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTC 660
           SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYF+FLCTQDLTP+QLKVFSK SNRTC
Sbjct: 601 SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTC 660

Query: 661 HRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEP 720
            RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPA SSY AVVT F GAAVKVEP
Sbjct: 661 RRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFKGAAVKVEP 720

Query: 721 ESLNFTRRYQKLSYRITFLSKKRQSMPEFGGLIWKDGSHKVRSPIVITWLSFV 773
           ESLNFTRRY+KLSYRITF++KKR SMPEFGGLIWKDGSHKVRSPIVITWLSFV
Sbjct: 721 ESLNFTRRYEKLSYRITFVTKKRLSMPEFGGLIWKDGSHKVRSPIVITWLSFV 773

[Close]
BLAST of Spg002787 vs. ExPASy TrEMBL
Match: A0A6J1J576 (subtilisin-like protease SBT1.3 OS=Cucurbita maxima OX=3661 GN=LOC111482733 PE=3 SV=1)

HSP 1 Score: 1459.5 bits (3777), Expect = 0.0e+00
Identity = 720/772 (93.26%), Postives = 746/772 (96.63%), Query Frame = 0 

Query: 1   MADSLVKWLCLLLFLHCLLLSRAQFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           MA + +KWLCLLL   C LLS AQFLKTYVVQMDRSAMP SFSDH QWYSTVL++VVV+P
Sbjct: 1   MAHTPLKWLCLLLVFDCSLLSSAQFLKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEERIIYSYQSVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPKF 120
           EREGNGGGE+RIIYSYQ+VFHGVAARLSEEEAERLEEEDGVLAIFPETKY+LHTTRSP+F
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYDLHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVP HWKGECETGR FT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           KQNCNRKIVGAR+FYHGYEAATGKFNE+LEYKSPRDQDGHGTHTAATVAGSP A A+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTASANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRT 360
           SLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+GRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLY+GRITIPENKQFPVVYMGSNSS+PDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKATA 480
           RVQKGVVVKNAGG+GMIL+NTAANGEELVADCHLVPAVAVGE+EGKAIK YALTNRKATA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKATA 480

Query: 481 TLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           +LGFLGT+LGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 SLGFLGTKLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPL+DSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 AASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTCH 660
             SPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSK SNR+CH
Sbjct: 601 GTSPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEPE 720
            LLPNPGDLNYPAISAVFPEK SVTSLTLHRTVTNVGPA SSY AV T FNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKASVTSLTLHRTVTNVGPATSSYHAVATPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYRITFLSKKRQSMPEFGGLIWKDGSHKVRSPIVITWLSFV 773
           SLNFTRRYQKLSY+ITFL+KKRQSMPEFGGLIWKDG+H VRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

[Close]
BLAST of Spg002787 vs. ExPASy TrEMBL
Match: A0A1S3AY00 (subtilisin-like protease SBT1.3 OS=Cucumis melo OX=3656 GN=LOC103484020 PE=3 SV=1)

HSP 1 Score: 1458.4 bits (3774), Expect = 0.0e+00
Identity = 725/773 (93.79%), Postives = 749/773 (96.90%), Query Frame = 0 

Query: 1   MADSLVKWLCLLLFLHCLLLSRAQFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           MAD  +K LCLLLFL  LLLS A FLKTYVVQMDRSAMPASF++HF+WYS VLSNVVV  
Sbjct: 1   MADFPLKCLCLLLFLDSLLLSSALFLKTYVVQMDRSAMPASFTNHFEWYSNVLSNVVVDL 60

Query: 61  EREGN-GGGEERIIYSYQSVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPK 120
           EREGN GGGEERIIYSYQ+VFHGVAARLSEEE E+LEEEDGV+AIFPE KYELHTTRSP+
Sbjct: 61  EREGNGGGGEERIIYSYQNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPR 120

Query: 121 FLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGF 180
           FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPES+SF+DAGMSPVP HWKGECETGRGF
Sbjct: 121 FLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGF 180

Query: 181 TKQNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240
           TKQNCNRKIVGARVFYHGY+AATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL
Sbjct: 181 TKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240

Query: 241 GYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300
           GYAYGTARGMAPGARIAAYKVCW+GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR
Sbjct: 241 GYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300

Query: 301 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 360
           DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR
Sbjct: 301 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 360

Query: 361 TVTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGIS 420
           T++G SLYRGRITIPENKQFP+VYMGSNSSSPDPSSLCLEGTLDPH VAGKIVICDRGIS
Sbjct: 361 TMSGASLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHVVAGKIVICDRGIS 420

Query: 421 PRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKAT 480
           PRVQKGVVVKNAGGIGMILSNT ANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKAT
Sbjct: 421 PRVQKGVVVKNAGGIGMILSNTVANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKAT 480

Query: 481 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 540
           ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT
Sbjct: 481 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 540

Query: 541 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDS 600
           DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAY+HDNTYKPL+DS
Sbjct: 541 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYIHDNTYKPLQDS 600

Query: 601 SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTC 660
           SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYF+FLCTQDLTP+QLKVFSK SNRTC
Sbjct: 601 SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTC 660

Query: 661 HRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEP 720
            RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPA SSY AVVT F GAAVKVEP
Sbjct: 661 RRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFKGAAVKVEP 720

Query: 721 ESLNFTRRYQKLSYRITFLSKKRQSMPEFGGLIWKDGSHKVRSPIVITWLSFV 773
           ESLNFTRRY+KLSYRITF++KKR SMPEFGGLIWKDGSHKVRSPIV+TWLSFV
Sbjct: 721 ESLNFTRRYEKLSYRITFVTKKRLSMPEFGGLIWKDGSHKVRSPIVMTWLSFV 773

[Close]
BLAST of Spg002787 vs. ExPASy TrEMBL
Match: A0A6J1FZU3 (subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111449392 PE=3 SV=1)

HSP 1 Score: 1446.0 bits (3742), Expect = 0.0e+00
Identity = 714/773 (92.37%), Postives = 747/773 (96.64%), Query Frame = 0 

Query: 1   MADSLVKWLCLLLFLHCLLLSRAQFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVV-S 60
           MA + ++WLC  LF HCL  S AQF KTYVVQMDRSAMP SFSDH +WYSTV+S+VVV +
Sbjct: 1   MAHTPLQWLCFPLFFHCLFFSSAQFFKTYVVQMDRSAMPDSFSDHLEWYSTVVSSVVVDN 60

Query: 61  PERE--GNGGGEERIIYSYQSVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRS 120
           PERE  GNGGGE+RIIYSYQ+VFHGVAARLSEEEAERLEEE GVLA+FPE KYELHTTRS
Sbjct: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120

Query: 121 PKFLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGR 180
           PKFLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVP +WKGECETGR
Sbjct: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180

Query: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGAS 240
           GFTKQNCNRKIVGARVFYHGYEAATGKFN+QLEYKSPRDQDGHGTHTAATVAGSPVAGA+
Sbjct: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGD 360
           YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+
Sbjct: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360

Query: 361 GRTVTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRG 420
           GRT+TGVSLYRGRITI ENKQFPVVYMGSNSS+PDPSSLCLEGTLDPHFVAGKIVICDRG
Sbjct: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420

Query: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRK 480
           ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHL+PAVAVGE EGKAIKQYALTNRK
Sbjct: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480

Query: 481 ATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540
            TATLG LGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL
Sbjct: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540

Query: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLR 600
           TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPL+
Sbjct: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600

Query: 601 DSSAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNR 660
           D+SAASPSSPY+HGAGHINPRKALDPGLVYEI+PQDYFEFLCTQDLTPSQLKVFSK+SNR
Sbjct: 601 DASAASPSSPYEHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660

Query: 661 TCHRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKV 720
           +CHRLLPNPGDLNYPAISAVFPEKT+VTSLTLHRTVTNVGPA+SSY A+V+ F GAAVK+
Sbjct: 661 SCHRLLPNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720

Query: 721 EPESLNFTRRYQKLSYRITFLSKKRQSMPEFGGLIWKDGSHKVRSPIVITWLS 771
           EPE LNFTRRYQKLSYRITF++KKR SMPEFGGLIWKDGSH+VRSPI+ITWLS
Sbjct: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLS 773

[Close]
BLAST of Spg002787 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 1161.7 bits (3004), Expect = 0.0e+00
Identity = 560/761 (73.59%), Postives = 658/761 (86.47%), Query Frame = 0 

Query: 12  LLFLHCLLLSRAQFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVV--VSPEREGNGGGE 71
           L+FL     ++    KTYV+ MD+SAMP  +++H QWYS+ +++V    S E EGN    
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGN---N 79

Query: 72  ERIIYSYQSVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSN 131
            RI+Y+YQ+ FHG+AA+L++EEAERLEEEDGV+A+ PET+YELHTTRSP FLGLE  +S 
Sbjct: 80  NRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESE 139

Query: 132 SAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNRKIV 191
             W++++ DHDV+VGVLDTGIWPESESFND GMSPVP  W+G CETG+ F K+NCNRKIV
Sbjct: 140 RVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIV 199

Query: 192 GARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGM 251
           GARVFY GYEAATGK +E+LEYKSPRD+DGHGTHTAATVAGSPV GA+L G+AYGTARGM
Sbjct: 200 GARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGM 259

Query: 252 APGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGA 311
           A  AR+AAYKVCWVGGCFSSDILSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FGA
Sbjct: 260 AQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGA 319

Query: 312 MEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRG 371
           MEMGVFVSCSAGNGGPDP+SLTNVSPWITTVGASTMDRDFPA VK+G  RT  GVSLY+G
Sbjct: 320 MEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKG 379

Query: 372 RITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVK 431
           R  +P+NKQ+P+VY+G N+SSPDP+S CL+G LD   VAGKIVICDRG++PRVQKG VVK
Sbjct: 380 RTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVK 439

Query: 432 NAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKATATLGFLGTRL 491
            AGGIGM+L+NTA NGEELVAD H++PAVAVGEKEGK IKQYA+T++KATA+L  LGTR+
Sbjct: 440 RAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRI 499

Query: 492 GVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNIL 551
           G+KPSPVVAAFSSRGPNFL+LEILKPDL+APGVNILAAWTG   PSSL++D RRVKFNIL
Sbjct: 500 GIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNIL 559

Query: 552 SGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDSSAASPSSPYD 611
           SGTSMSCPHVSGVAALIKS+HPDWSP+AIKSALMTTAYVHDN +KPL D+S A+PSSPYD
Sbjct: 560 SGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYD 619

Query: 612 HGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTC-HRLLPNPGD 671
           HGAGHI+P +A DPGLVY+I PQ+YFEFLCTQDL+PSQLKVF+K+SNRTC H L  NPG+
Sbjct: 620 HGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGN 679

Query: 672 LNYPAISAVFPEKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEPESLNFTRRY 731
           LNYPAISA+FPE T V ++TL RTVTNVGP  SSY+  V+ F GA+V V+P++LNFT ++
Sbjct: 680 LNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKH 739

Query: 732 QKLSYRITFLSKKRQSMPEFGGLIWKDGSHKVRSPIVITWL 770
           QKLSY +TF ++ R   PEFGGL+WK  +HKVRSP++ITWL
Sbjct: 740 QKLSYTVTFRTRFRMKRPEFGGLVWKSTTHKVRSPVIITWL 777

[Close]
BLAST of Spg002787 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 803.1 bits (2073), Expect = 1.9e-232
Identity = 412/768 (53.65%), Postives = 531/768 (69.14%), Query Frame = 0 

Query: 4   SLVKWLCLLLFLHCLLLSRAQFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPERE 63
           S   +  LL    C + S +    TY+V M +S MP+SF  H  WY + L ++  S E  
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE-- 66

Query: 64  GNGGGEERIIYSYQSVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPKFLGL 123
                   ++Y+Y++  HG + RL++EEA+ L  + GV+++ PE +YELHTTR+P FLGL
Sbjct: 67  --------LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGL 126

Query: 124 EPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQN 183
           +   ++  + +  +  DV+VGVLDTG+WPES+S++D G  P+P  WKG CE G  FT   
Sbjct: 127 DEHTAD-LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASL 186

Query: 184 CNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAY 243
           CNRK++GAR F  GYE+  G  +E  E +SPRD DGHGTHT++T AGS V GASLLGYA 
Sbjct: 187 CNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYAS 246

Query: 244 GTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLS 303
           GTARGMAP AR+A YKVCW+GGCFSSDIL+A+D+A+AD VNVLS+SLGGG+S YYRD ++
Sbjct: 247 GTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVA 306

Query: 304 VAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTG 363
           + AF AME G+ VSCSAGN GP   SL+NV+PWITTVGA T+DRDFPA+  LG+G+  TG
Sbjct: 307 IGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTG 366

Query: 364 VSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQ 423
           VSL++G   +P+ K  P +Y G N+S+    +LC+ GTL P  V GKIV+CDRGI+ RVQ
Sbjct: 367 VSLFKGE-ALPD-KLLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQ 426

Query: 424 KGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKATATLG 483
           KG VVK AGG+GMIL+NTAANGEELVAD HL+PA  VGEK G  I+ Y  T+   TA++ 
Sbjct: 427 KGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASIS 486

Query: 484 FLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRR 543
            LGT +GVKPSPVVAAFSSRGPN +T  ILKPDL+APGVNILAAWTG  GP+ L +D+RR
Sbjct: 487 ILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRR 546

Query: 544 VKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDSSAAS 603
           V+FNI+SGTSMSCPHVSG+AAL+KS HP+WSP+AI+SALMTTAY      KPL D +   
Sbjct: 547 VEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGK 606

Query: 604 PSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTCHRLL 663
           PS+P+DHGAGH++P  A +PGL+Y++  +DY  FLC  + T  Q++  S+  N TC    
Sbjct: 607 PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRR-NYTCDPSK 666

Query: 664 P-NPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEPESL 723
             +  DLNYP+ +        V +    RTVT+VG A +    V +   G  + VEP  L
Sbjct: 667 SYSVADLNYPSFAV---NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVL 726

Query: 724 NFTRRYQKLSYRITFL--SKKRQSMPEFGGLIWKDGSHKVRSPIVITW 769
           NF    +K SY +TF   S K      FG + W DG H V SP+ I+W
Sbjct: 727 NFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

[Close]
BLAST of Spg002787 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 768.1 bits (1982), Expect = 6.8e-222
Identity = 396/763 (51.90%), Postives = 517/763 (67.76%), Query Frame = 0 

Query: 14  FLHCLLLSRAQFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPEREGNGGGEERII 73
           FL  LL + A+  KTY+++++ S  P SF  H  WY++ L++             E  ++
Sbjct: 17  FLFLLLHTTAK--KTYIIRVNHSDKPESFLTHHDWYTSQLNS-------------ESSLL 76

Query: 74  YSYQSVFHGVAARLSEEEAER-LEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNSAW 133
           Y+Y + FHG +A L   EA+  L   + +L IF +  Y LHTTR+P+FLGL         
Sbjct: 77  YTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN--SEFGVH 136

Query: 134 SQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNRKIVGAR 193
               + + VI+GVLDTG+WPES SF+D  M  +P  WKGECE+G  F  + CN+K++GAR
Sbjct: 137 DLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGAR 196

Query: 194 VFYHGYEAAT-GKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAP 253
            F  G++ A+ G F+ + E  SPRD DGHGTHT+ T AGS V  AS LGYA GTARGMA 
Sbjct: 197 SFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMAT 256

Query: 254 GARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAME 313
            AR+A YKVCW  GCF SDIL+A+DRA+ DGV+VLS+SLGGG + YYRD++++ AF AME
Sbjct: 257 RARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAME 316

Query: 314 MGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGRI 373
            GVFVSCSAGN GP   S+ NV+PW+ TVGA T+DRDFPA   LG+G+ +TGVSLY G  
Sbjct: 317 RGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG-- 376

Query: 374 TIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNA 433
                K   +VY   NSSS   S+LCL G+LD   V GKIV+CDRG++ RV+KG VV++A
Sbjct: 377 VGMGTKPLELVYNKGNSSS---SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDA 436

Query: 434 GGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKATATLGFLGTRLGV 493
           GG+GMI++NTAA+GEELVAD HL+PA+AVG+K G  +++Y  ++ K TA L F GT L V
Sbjct: 437 GGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDV 496

Query: 494 KPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSG 553
           KPSPVVAAFSSRGPN +T EILKPD++ PGVNILA W+   GP+ L  D+RR +FNI+SG
Sbjct: 497 KPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSG 556

Query: 554 TSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDSSAASPSSPYDHG 613
           TSMSCPH+SG+A L+K+ HP+WSPSAIKSALMTTAYV DNT  PL D++  S S+PY HG
Sbjct: 557 TSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHG 616

Query: 614 AGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTCHRLLPNPGDLNY 673
           +GH++P+KAL PGLVY+I  ++Y  FLC+ D T   +    K  +  C +   +PG LNY
Sbjct: 617 SGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNY 676

Query: 674 PAISAVFPEKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEPESLNFTRRYQKL 733
           P+ S +F  K  V      R VTNVG A+S Y+  V       + V+P  L+F    +K 
Sbjct: 677 PSFSVLFGGKRVV---RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKK 736

Query: 734 SYRITFLSKKRQSM---PEFGGLIWKDGSHKVRSPIVITWLSF 772
            Y +TF+SKK  SM    EFG + W +  H+VRSP+  +W  F
Sbjct: 737 RYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNRF 754

[Close]
BLAST of Spg002787 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 751.5 bits (1939), Expect = 6.6e-217
Identity = 381/754 (50.53%), Postives = 516/754 (68.44%), Query Frame = 0 

Query: 28  TYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPEREGNGGGEERIIYSYQSVFHGVAARL 87
           TY+V +D  A P+ F  HF WY++ L+++  SP           II++Y +VFHG +ARL
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP---------PSIIHTYDTVFHGFSARL 86

Query: 88  SEEEAERLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNSAWSQQIADHDVIVGVLD 147
           + ++A +L +   V+++ PE    LHTTRSP+FLGL   D      +     D+++GV+D
Sbjct: 87  TSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVID 146

Query: 148 TGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNRKIVGARVFYHGYEAATGKFNE 207
           TG+WPE  SF+D G+ PVP  WKG+C   + F +  CNRK+VGAR F  GYEA  GK NE
Sbjct: 147 TGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNE 206

Query: 208 QLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWVGGCF 267
             E++SPRD DGHGTHTA+  AG  V  AS LGYA+G A GMAP AR+AAYKVCW  GC+
Sbjct: 207 TTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCY 266

Query: 268 SSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDP 327
            SDIL+A D AVADGV+V+S+S+GG V  YY D++++ AFGA++ G+FVS SAGNGGP  
Sbjct: 267 DSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGA 326

Query: 328 VSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGRITIPENKQFPVVYMGSN 387
           +++TNV+PW+TTVGA T+DRDFPA VKLG+G+ ++GVS+Y G    P  + +P+VY GS 
Sbjct: 327 LTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDP-GRMYPLVYGGSL 386

Query: 388 SSSPD-PSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGE 447
                  SSLCLEG+LDP+ V GKIV+CDRGI+ R  KG +V+  GG+GMI++N   +GE
Sbjct: 387 LGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGE 446

Query: 448 ELVADCHLVPAVAVGEKEGKAIKQYALTNRKA------TATLGFLGTRLGVKPSPVVAAF 507
            LVADCH++PA +VG   G  I++Y   + K+      TAT+ F GTRLG++P+PVVA+F
Sbjct: 447 GLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASF 506

Query: 508 SSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVS 567
           S+RGPN  T EILKPD++APG+NILAAW  + GPS +T+D RR +FNILSGTSM+CPHVS
Sbjct: 507 SARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVS 566

Query: 568 GVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDSSAASPSSPYDHGAGHINPRKA 627
           G+AAL+K+ HPDWSP+AI+SAL+TTAY  DN+ +P+ D S  + SS  D+G+GH++P KA
Sbjct: 567 GLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKA 626

Query: 628 LDPGLVYEIQPQDYFEFLCTQDLTPSQL-KVFSKNSNRTCHRLLPNPGDLNYPAISAVFP 687
           +DPGLVY+I   DY  FLC  + T + +  +  + ++    R   + G+LNYP+ S VF 
Sbjct: 627 MDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQ 686

Query: 688 E-KTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEPESLNFTRRYQKLSYRITFL 747
           +   S  S    RTVTNVG + S Y   +    G  V VEPE L+F R  QKLS+ +   
Sbjct: 687 QYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVK 746

Query: 748 SKKRQSMP-----EFGGLIWKDGSHKVRSPIVIT 768
           + + +  P     E G ++W DG   V SP+V+T
Sbjct: 747 TTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

[Close]
BLAST of Spg002787 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 749.6 bits (1934), Expect = 2.5e-216
Identity = 396/774 (51.16%), Postives = 522/774 (67.44%), Query Frame = 0 

Query: 9   LCLLLFLHCLLLSRA--QFLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPEREGNG 68
           + LLLFL    +S A  Q  KT++ ++D  +MP+ F  H+ WYST  +            
Sbjct: 6   IVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAE----------- 65

Query: 69  GGEERIIYSYQSVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPKFLGLEPA 128
             E RI++ Y +VFHG +A ++ +EA+ L     VLA+F + + ELHTTRSP+FLGL+  
Sbjct: 66  --ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ-- 125

Query: 129 DSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNR 188
           +    WS+     DVI+GV DTGIWPE  SF+D  + P+P  W+G CE+G  F+ +NCNR
Sbjct: 126 NQKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNR 185

Query: 189 KIVGARVFYHGYEAAT-GKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGT 248
           KI+GAR F  G +AA  G  N+ +E+ SPRD DGHGTHT++T AG     AS+ GYA G 
Sbjct: 186 KIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGV 245

Query: 249 ARGMAPGARIAAYKVCWV-GGCFSSDILSAVDRAVADGVNVLSISLGGG---VSSYYRDS 308
           A+G+AP ARIAAYKVCW   GC  SDIL+A D AV DGV+V+SIS+GGG    S YY D 
Sbjct: 246 AKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDP 305

Query: 309 LSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTV 368
           +++ ++GA   G+FVS SAGN GP+ +S+TN++PW+TTVGAST+DR+FPA   LGDG  +
Sbjct: 306 IAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRL 365

Query: 369 TGVSLYRGRITIPEN-KQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISP 428
            GVSLY G   +P N + FPVVY G +  S   +SLC+E TLDP  V GKIVICDRG SP
Sbjct: 366 RGVSLYAG---VPLNGRMFPVVYPGKSGMS--SASLCMENTLDPKQVRGKIVICDRGSSP 425

Query: 429 RVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKATA 488
           RV KG+VVK AGG+GMIL+N A+NGE LV D HL+PA AVG  EG  IK YA ++    A
Sbjct: 426 RVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIA 485

Query: 489 TLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 548
           ++ F GT +G+KP+PV+A+FS RGPN L+ EILKPDL+APGVNILAAWT   GP+ L +D
Sbjct: 486 SIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSD 545

Query: 549 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLRDSS 608
            R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSP+ I+SA+MTT  + DN+ + L D S
Sbjct: 546 PRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDES 605

Query: 609 AASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKNSNRTCH 668
               ++PYD+G+GH+N  +A++PGLVY+I   DY  FLC+    P  ++V ++   R   
Sbjct: 606 TGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPT 665

Query: 669 RLLPNPGDLNYPAISAVFP-EKTSVTSLTLHRTVTNVGPAASSYRAVVTLFNGAAVKVEP 728
              P+PG+LNYP+I+AVFP  +  + S T+ RT TNVG A + YRA +    G  V V+P
Sbjct: 666 TRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKP 725

Query: 729 ESLNFTRRYQKLSYRITFLSKKR-----QSMPEFGGLIWKD-GSHKVRSPIVIT 768
             L FT   ++ SY +T     R     ++   FG + W D G H VRSPIV+T
Sbjct: 726 PRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

The following BLAST results are available for this feature:
BLAST of Spg002787 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG6576764.10.0e+0093.65Subtilisin-like protease 1.3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022922929.10.0e+0093.78subtilisin-like protease SBT1.3 [Cucurbita moschata][more]
XP_023552616.10.0e+0093.78subtilisin-like protease SBT1.3 [Cucurbita pepo subsp. pepo][more]
KAA0033586.10.0e+0094.18subtilisin-like protease SBT1.3 [Cucumis melo var. makuwa] >TYK22252.1 subtilisi... [more]
XP_022984431.10.0e+0093.26subtilisin-like protease SBT1.3 [Cucurbita maxima][more]
BLAST of Spg002787 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9FLI40.0e+0073.59Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S... [more]
O653512.7e-23153.65Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
Q9ZUF69.6e-22151.90Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
Q9LUM39.3e-21650.53Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... [more]
O496073.5e-21551.16Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... [more]
BLAST of Spg002787 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1E4V20.0e+0093.78subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111430759 PE... [more]
A0A5A7SUB40.0e+0094.18Subtilisin-like protease SBT1.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A6J1J5760.0e+0093.26subtilisin-like protease SBT1.3 OS=Cucurbita maxima OX=3661 GN=LOC111482733 PE=3... [more]
A0A1S3AY000.0e+0093.79subtilisin-like protease SBT1.3 OS=Cucumis melo OX=3656 GN=LOC103484020 PE=3 SV=... [more]
A0A6J1FZU30.0e+0092.37subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111449392 PE... [more]
BLAST of Spg002787 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G51750.10.0e+0073.59subtilase 1.3 [more]
AT5G67360.11.9e-23253.65Subtilase family protein [more]
AT2G05920.16.8e-22251.90Subtilase family protein [more]
AT3G14240.16.6e-21750.53Subtilase family protein [more]
AT4G34980.12.5e-21651.16subtilisin-like serine protease 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 216..229
score: 50.79
coord: 550..566
score: 58.72
coord: 138..157
score: 31.6
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 668..766
e-value: 2.3E-27
score: 95.1
NoneNo IPR availableGENE3D3.50.30.30coord: 346..490
e-value: 1.0E-184
score: 616.6
NoneNo IPR availableGENE3D2.60.40.2310coord: 638..769
e-value: 2.3E-34
score: 119.9
NoneNo IPR availablePANTHERPTHR10795:SF675SUBTILISIN-LIKE PROTEASE SBT1.7coord: 18..767
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 109..621
score: 30.829422
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 353..482
e-value: 5.24389E-37
score: 132.923
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 28..113
e-value: 1.3E-16
score: 61.0
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 140..634
e-value: 1.0E-184
score: 616.6
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 112..625
IPR003137PA domainPFAMPF02225PAcoord: 380..468
e-value: 3.7E-9
score: 36.5
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 139..592
e-value: 4.8E-52
score: 177.4
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 20..113
e-value: 3.8E-19
score: 70.9
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 18..767
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 551..561
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 110..587
e-value: 1.4928E-154
score: 450.126

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg002787.1Spg002787.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity