Spg002375 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg002375 vs. NCBI nr
Match: KAG7014677.1 (Subtilisin-like protease SBT3.3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1396.3 bits (3613), Expect = 0.0e+00
Identity = 686/773 (88.75%), Postives = 733/773 (94.83%), Query Frame = 0

Query: 376  MSSFAHGKSSRTTIFLVFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKT 435
            M SFAHGKSS TTIFLV IS FTAL+ LCTL I   AKSNVYIVYMG +PHDNEELLVK 
Sbjct: 1    MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 436  HHGVLASVLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQ 495
            HHGVLASVLGSQE S DSLVY+YKYGFSGFAAKLTMAQAQM+SELPSV++VIPNRLHKMQ
Sbjct: 61   HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120

Query: 496  TTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGIC 555
            TTRSWDYLQLSPRFPNSLL+KSRMG+GAIIG+LDTGIWPESEVFCD+GLGPVPSRWKGIC
Sbjct: 121  TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 556  ESGELFNPAKACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAG 615
            ESGELF+PAKACSRKLIGARYF+KGLEAAYGHP NN+ FQDY SPRD SGHGTHVSSVA 
Sbjct: 181  ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240

Query: 616  GSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVL 675
            G FVPNV YHGLAVGTVRGGAP SR+AMYKVCW++NGGVC+DIDILKAIDQAIYDGVDVL
Sbjct: 241  GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 676  SLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAA 735
            SLSLGP+FPSYSDVDM +GIAIGAFHAVAKGI VVGAAGNSGPAAYSV+NIEPWLLTVAA
Sbjct: 301  SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 736  SSVDRSFLVAVTLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAA 795
            SSVDRSFLVA+TLGNNWTTMGQGMFSGKLT+FHNLVYPEVSDLDD RACESLSLNDTWAA
Sbjct: 361  SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420

Query: 796  GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQ 855
            GNVVLCFASDDY+D+TH+TS SVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLD+GMQ
Sbjct: 421  GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480

Query: 856  ILNYIRSTRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 915
            ILNYIRSTRNPQVKIG STTRVGQ  STTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481  ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 916  VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGE 975
            VPPSDPK+RNSYAFISGTSMATPHVSAIVALLK+LH+HWSPAAI+SAIVTTAW+SDPYG 
Sbjct: 541  VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 976  VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 1035
            VIFAEG+PM SA PFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCA G+N+SAISGITK
Sbjct: 601  VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 660

Query: 1036 RSITCPKRRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAISVK 1095
            RSI+CPKRRPSILDINVPSITIPSL H ++LTRTV NVG +NS+YKAAI  PPGI I++K
Sbjct: 661  RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 720

Query: 1096 PRILKFNHKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            PRILKFNHKMK ISFTVT+S+NHRVTTGYCFGSLTWLDG+HSVRIP+SVRTNI
Sbjct: 721  PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Spg002375 vs. NCBI nr
Match: XP_022922598.1 (subtilisin-like protease SBT3.9 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1395.6 bits (3611), Expect = 0.0e+00
Identity = 687/773 (88.87%), Postives = 732/773 (94.70%), Query Frame = 0

Query: 376  MSSFAHGKSSRTTIFLVFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKT 435
            M SFAHGKSS TTIFLV IS FTAL+ LCTL I   AKSNVYIVYMG +PHDNEELLVK 
Sbjct: 1    MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 436  HHGVLASVLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQ 495
            HHGVLASVLGSQE S DSLVY+YKYGFSGFAAKLTMAQAQM+SELPSV++VIPNRLHKMQ
Sbjct: 61   HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120

Query: 496  TTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGIC 555
            TTRSWDYLQLSPRFPNSLL+KSRMG+GAIIG+LDTGIWPESEVFCD+GLGPVPSRWKGIC
Sbjct: 121  TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 556  ESGELFNPAKACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAG 615
            ESGELF+PAKACSRKLIGARYF+KGLEAAYGHP NN+ FQDY SPRD SGHGTHVSSVA 
Sbjct: 181  ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240

Query: 616  GSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVL 675
            G FVPNV YHGLAVGTVRGGAP SR+AMYKVCW++NGGVC+DIDILKAIDQAIYDGVDVL
Sbjct: 241  GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 676  SLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAA 735
            SLSLGP+FPSYSDVDM +GIAIGAFHAVAKGI VVGAAGNSGPAAYSV+NIEPWLLTVAA
Sbjct: 301  SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 736  SSVDRSFLVAVTLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAA 795
            SSVD SFLVA+TLGNNWTTMGQGMFSGKLT+FHNLVYPEVSDLDD RACESLSLNDTWAA
Sbjct: 361  SSVDCSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420

Query: 796  GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQ 855
            GNVVLCFASDDY+D+TH+TS SVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLD+GMQ
Sbjct: 421  GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480

Query: 856  ILNYIRSTRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 915
            ILNYIRSTRNPQVKIG STTRVGQ  STTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481  ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 916  VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGE 975
            VPPSDPK+RNSYAFISGTSMATPHVSAIVALLK+LH+HWSPAAI+SAIVTTAW+SDPYG 
Sbjct: 541  VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 976  VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 1035
            VIFAEG+PM SA PFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCA G+NNSAISGITK
Sbjct: 601  VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITK 660

Query: 1036 RSITCPKRRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAISVK 1095
            RSI+CPKRRPSILDINVPSITIPSL H ++LTRTV NVG +NS+YKAAI  PPGI I+VK
Sbjct: 661  RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAVK 720

Query: 1096 PRILKFNHKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            PRILKFNHKMK ISFTVT+S+NHRVTTGYCFGSLTWLDG+HSVRIP+SVRTNI
Sbjct: 721  PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Spg002375 vs. NCBI nr
Match: XP_022141072.1 (subtilisin-like protease SBT3.9 [Momordica charantia])

HSP 1 Score: 1394.8 bits (3609), Expect = 0.0e+00
Identity = 685/775 (88.39%), Postives = 732/775 (94.45%), Query Frame = 0

Query: 376  MSSFAHGKSSRTTIFLVFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKT 435
            MSSFAHGKSS TTI L+FISFFT L+DLCTLL+  DAKS+VY+VYMG RPHDNEELLVKT
Sbjct: 1    MSSFAHGKSSSTTILLLFISFFTLLSDLCTLLVPVDAKSHVYVVYMGKRPHDNEELLVKT 60

Query: 436  HHGVLASVLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQ 495
            HHGVLASVLGSQEA ADS+VYNYKYGFSGFAAKLTMAQAQ +SELP+VIKVIPNRLHKMQ
Sbjct: 61   HHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQKISELPTVIKVIPNRLHKMQ 120

Query: 496  TTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGIC 555
            TTRSWDYLQLSPR  NSLLQKSRMGNGAIIGLLDTGIWPESEVF D+GLGPVPSRWKGIC
Sbjct: 121  TTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTGIWPESEVFSDNGLGPVPSRWKGIC 180

Query: 556  ESGELFNPAKACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAG 615
            ESGELF+P KACSRKLIGARYFIKGLEAAYGHP+NN+GFQDYLSPRDY+GHGTHVSSVA 
Sbjct: 181  ESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGFQDYLSPRDYAGHGTHVSSVAS 240

Query: 616  GSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVL 675
            GSFV NV YHGLAVGTVRGGAPQSRLAMYKVCW+LNGGVCSD DILKAIDQAI+DGVDVL
Sbjct: 241  GSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGVCSDADILKAIDQAIHDGVDVL 300

Query: 676  SLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAA 735
            SLSLGPTFPSYSDVDMH+G+AIG FHA+ KGI VVGAAGN GPAAYSVANIEPWLLTVAA
Sbjct: 301  SLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAGNYGPAAYSVANIEPWLLTVAA 360

Query: 736  SSVDRSFLVAVTLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAA 795
            SSVDRSFLVA+TLGNNWTT+GQGMFSGKL RFHNLVYPEVSDL+D RACESLSLNDTWAA
Sbjct: 361  SSVDRSFLVAITLGNNWTTVGQGMFSGKLRRFHNLVYPEVSDLEDERACESLSLNDTWAA 420

Query: 796  GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQ 855
            GNVVLCFASDD++DNTH TS SVKKVG LGLIVAKNPTK VEPFIN+FPCVQ+SL++GM 
Sbjct: 421  GNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTKDVEPFINHFPCVQVSLEIGMH 480

Query: 856  ILNYIRSTRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 915
            ILNYIRSTRNPQV IGPS TR+GQS STTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481  ILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 916  VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGE 975
            VPPSDPK++NSYAFISGTSMATPHVSAIVALLKSLH+HWSPAAI+SAIVTTAWTSDPYGE
Sbjct: 541  VPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 600

Query: 976  VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 1035
             IFAEGRP+KSAGPFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCAMG+N+SAISGITK
Sbjct: 601  TIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMADYIRYFCAMGYNDSAISGITK 660

Query: 1036 RSITCPKRRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPP--PGIAIS 1095
            + I+CPKRRPSILD NVPSITIP L+HS+ +TRTV NVG VNSTYKAAI  P  PGIAI+
Sbjct: 661  KPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVGAVNSTYKAAIETPPGPGIAIA 720

Query: 1096 VKPRILKFNHKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            VKPR+LKFNHK K ISFTVTVS+NHRVTTGYCFGSLTWLDG+HSVRIPISVRT+I
Sbjct: 721  VKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWLDGVHSVRIPISVRTDI 775

BLAST of Spg002375 vs. NCBI nr
Match: XP_022984356.1 (subtilisin-like protease SBT3.9 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1394.8 bits (3609), Expect = 0.0e+00
Identity = 686/773 (88.75%), Postives = 732/773 (94.70%), Query Frame = 0

Query: 376  MSSFAHGKSSRTTIFLVFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKT 435
            M SFAHGKSS TTIFLV IS FTAL+ LCTL I   AKSNVYIVYMG +PHDNEELLVK 
Sbjct: 1    MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 436  HHGVLASVLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQ 495
            HHGVLASVLGSQE S DSLVY+YKYGFSGFAAKLTMAQAQM+SELPSV++VIPNRLHK+Q
Sbjct: 61   HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKIQ 120

Query: 496  TTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGIC 555
            TTRSWDYLQLSPRFPNSLL+KSRMG+GAIIG+LDTGIWPESEVFCD+GLGPVPSRWKGIC
Sbjct: 121  TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 556  ESGELFNPAKACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAG 615
            ESGELF+PAKACSRKLIGARYF+KGLEAAYGHP NN+ FQDYLSPRD SGHGTHVSSVA 
Sbjct: 181  ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYLSPRDRSGHGTHVSSVAS 240

Query: 616  GSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVL 675
            G FVPNV YHGLAVGTVRGGAP SR+AMYKVCW++NGGVC+DIDILKAIDQAIYDGVDVL
Sbjct: 241  GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 676  SLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAA 735
            SLSLGP+FPSYSDVDM +GIAIGAFHAVAKGI VVGAAGNSGPAAYSV+NIEPWLLTVAA
Sbjct: 301  SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 736  SSVDRSFLVAVTLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAA 795
            SSVDRSFLVA+TLGNNWTTMGQGMFSGKLT+F NLVYPEVSDLDD RACESLSLNDTWA+
Sbjct: 361  SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFINLVYPEVSDLDDERACESLSLNDTWAS 420

Query: 796  GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQ 855
            GNVVLCFASDDY+DNTH+TSWSVKKVGGLGLIVAKNPTKAVEPFI+NFPCVQISLD+GMQ
Sbjct: 421  GNVVLCFASDDYNDNTHNTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDIGMQ 480

Query: 856  ILNYIRSTRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 915
            ILNYIRSTRNPQVKIG STTRVGQ  STTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481  ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 916  VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGE 975
            VPPSDPK RNSYAFISGTSMATPHVSAIVALLK+LH+HWSPAAI+SAIVTTAW+SDPYG 
Sbjct: 541  VPPSDPKNRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 976  VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 1035
            VIFAEGRPM SA PFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCA G+NNSAISGI+K
Sbjct: 601  VIFAEGRPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGISK 660

Query: 1036 RSITCPKRRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAISVK 1095
            RSI+CPKRRPSILDINVPSITIPSL HS++LTRTV NVG +NS+YKAAI  PPGI I+VK
Sbjct: 661  RSISCPKRRPSILDINVPSITIPSLTHSVSLTRTVTNVGAINSSYKAAIEVPPGITIAVK 720

Query: 1096 PRILKFNHKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            PRILKFNHK K ISFTVT+S+N RVTTGYCFGSLTWLDG+HSVRIP+SVRTNI
Sbjct: 721  PRILKFNHKTKSISFTVTISSNQRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Spg002375 vs. NCBI nr
Match: XP_023551712.1 (subtilisin-like protease SBT3.9 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1392.5 bits (3603), Expect = 0.0e+00
Identity = 685/773 (88.62%), Postives = 731/773 (94.57%), Query Frame = 0

Query: 376  MSSFAHGKSSRTTIFLVFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKT 435
            M SFAHGKSS TTIFLV IS FTAL+ LCTL I   AKSNVYIVYMG +PHDNEELLVK 
Sbjct: 1    MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 436  HHGVLASVLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQ 495
            HHGVLASVLGSQE S DSLVY+YKYGFSGFAAKLTMAQAQM+SELPSV++VIPNRLHK+Q
Sbjct: 61   HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKIQ 120

Query: 496  TTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGIC 555
            TTRSWDYLQLSPRFPNSLL+KSRMG+GAIIG+LDTGIWPESEVFCD+GLGPVPSRWKGIC
Sbjct: 121  TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 556  ESGELFNPAKACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAG 615
            ESGELF+PAKACSRKLIGARYF+KGLEAAYGHP NN+ FQDY SPRD SGHGTHVSSVA 
Sbjct: 181  ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSKFQDYWSPRDRSGHGTHVSSVAS 240

Query: 616  GSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVL 675
            G FVPNV YHGLAVGTVRGGAP SR+AMYKVCW++NGGVC+DIDILKAIDQAIYDGVDVL
Sbjct: 241  GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 676  SLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAA 735
            SLSLGP+FPSYSDVDM +GIAIGAFHAVAKGI VVGAAGNSGPAAYSV+NIEPWLLTVAA
Sbjct: 301  SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 736  SSVDRSFLVAVTLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAA 795
            SSVDRSFLVA+TLGNNWTTMGQGMFSGKLT+FHNLVYPEVSDLDD RACESLSLNDTWAA
Sbjct: 361  SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420

Query: 796  GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQ 855
            GNVVLCFASDD++D+TH+TS SVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLD GMQ
Sbjct: 421  GNVVLCFASDDFNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDTGMQ 480

Query: 856  ILNYIRSTRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 915
            ILNYIRSTRNPQVKIG STTRVGQ  STTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481  ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 916  VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGE 975
            VPPSDPK+RNSYAFISGTSMATPHVSAIVALLK+LH+HWSPAAI+SAIVTTAW+SDPYG 
Sbjct: 541  VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 976  VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 1035
            VIFAEG+PM SA PFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCA G+NNSAISGITK
Sbjct: 601  VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITK 660

Query: 1036 RSITCPKRRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAISVK 1095
            RSI+CPKRRPSILDINVPSITIPSL H ++LTRTV NVG +NS+YKAAI  PPGI I+VK
Sbjct: 661  RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAVK 720

Query: 1096 PRILKFNHKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            PRILKFNHKMK I FTVT+S+NHRVTTGYCFGSLTWLDG+HSVRIP+SVRTNI
Sbjct: 721  PRILKFNHKMKSIPFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Spg002375 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 822.0 bits (2122), Expect = 8.4e-237
Identity = 418/773 (54.08%), Postives = 548/773 (70.89%), Query Frame = 0

Query: 383  KSSRTTIFLVFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLAS 442
            +S R++I LV +S  T LN        ++ +S V+IVY+G + H + E + ++HH +LAS
Sbjct: 2    RSFRSSILLVLLSLITVLN---ATRARSETESKVHIVYLGEKKHHDPEFVTESHHQMLAS 61

Query: 443  VLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDY 502
            +LGS++ + DS+VY+Y++GFSGFAAKLT +QA+ +++LP V+ VIP+  H++ TTR+W+Y
Sbjct: 62   LLGSKKDADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELATTRTWEY 121

Query: 503  LQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICESGELFN 562
            L LS   P +LL  + MG+  IIG++DTG+WPESE F D+G+GP+P +WKG CESGE F 
Sbjct: 122  LGLSSANPKNLLNDTNMGDQVIIGVIDTGVWPESESFNDNGVGPIPRKWKGGCESGENFR 181

Query: 563  PAKACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNV 622
             +  C+RKLIGA+YFI G   A    FN T  +DY+S RD+ GHGTHV+S+AGGSFVPNV
Sbjct: 182  -STDCNRKLIGAKYFINGF-LAENKGFNTTESRDYISARDFDGHGTHVASIAGGSFVPNV 241

Query: 623  GYHGLAVGTVRGGAPQSRLAMYKVCW---RLNGGVCSDIDILKAIDQAIYDGVDVLSLSL 682
             Y GLA GT+RGGAP++R+AMYK CW    L G  CSD DI+KAID+AI+DGVDVLS+SL
Sbjct: 242  SYKGLAGGTLRGGAPRARIAMYKACWFHEELKGVTCSDSDIMKAIDEAIHDGVDVLSISL 301

Query: 683  GPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVD 742
                P  S+ D+ D  A G FHAVAKGI VV A GN GPAA +V NI PW+LTVAA+++D
Sbjct: 302  VGQIPLNSETDIRDEFATGLFHAVAKGIVVVCAGGNDGPAAQTVVNIAPWILTVAATTLD 361

Query: 743  RSFLVAVTLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPR---ACESLSLNDTWA-A 802
            RSF   +TLGNN   +GQ  ++G      +LVYPE +  ++      CESL+LN  +  A
Sbjct: 362  RSFPTPITLGNNKVILGQATYTGPELGLTSLVYPENARNNNETFSGVCESLNLNPNYTMA 421

Query: 803  GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQ 862
              VVLCF +   +      +  VK  GGLGLI+++NP   + P  ++FPCV +  ++G  
Sbjct: 422  MKVVLCFTASRTNAAISRAASFVKAAGGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTD 481

Query: 863  ILNYIRSTRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 922
            IL+YIRSTR+P VKI  S T  GQ   T V  FSSRGPNS++PAILKPDIAAPGV ILAA
Sbjct: 482  ILSYIRSTRSPVVKIQRSRTLSGQPVGTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAA 541

Query: 923  VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGE 982
              P+D      +A +SGTSMATP +S ++ALLK+LH  WSPAA RSAIVTTAW +DP+GE
Sbjct: 542  TSPNDTLNVGGFAMLSGTSMATPVISGVIALLKALHPEWSPAAFRSAIVTTAWRTDPFGE 601

Query: 983  VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 1042
             IFAEG   K + PFD+GGGIVNP KA +PGL+YDMG  DYI Y C+ G+N+S+IS +  
Sbjct: 602  QIFAEGSSRKVSDPFDYGGGIVNPEKAAEPGLIYDMGPQDYILYLCSAGYNDSSISQLVG 661

Query: 1043 RSITCPKRRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAISVK 1102
            +   C   +PS+LD+N+PSITIP+L+  + LTRTV NVGLV+S YK ++ PP G+ + V 
Sbjct: 662  QITVCSNPKPSVLDVNLPSITIPNLKDEVTLTRTVTNVGLVDSVYKVSVEPPLGVRVVVT 721

Query: 1103 PRILKFNHKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            P  L FN K   +SFTV VST H++ TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 722  PETLVFNSKTISVSFTVRVSTTHKINTGYYFGSLTWTDSVHNVVIPLSVRTQI 769

BLAST of Spg002375 vs. ExPASy Swiss-Prot
Match: Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 1.3e-234
Identity = 426/766 (55.61%), Postives = 550/766 (71.80%), Query Frame = 0

Query: 385  SRTTIFL-VFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLASV 444
            S+T +FL +F+S    LN   + ++   A+S VY+VY+G + HDN E + ++HH +L S+
Sbjct: 2    SKTILFLALFLSI--VLNVQISFVV---AESKVYVVYLGEKEHDNPESVTESHHQMLWSL 61

Query: 445  LGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDYL 504
            LGS+EA  DS+VY+Y++GFSGFAAKLT +QAQ +SELP V++VIPN L++M TTR+WDYL
Sbjct: 62   LGSKEAVLDSIVYSYRHGFSGFAAKLTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYL 121

Query: 505  QLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICESGELFNP 564
             +SP   +SLLQK+ MG   I+G++D+G+WPESE+F D G GP+PSRWKG CESGELFN 
Sbjct: 122  GVSPGNSDSLLQKANMGYNVIVGVIDSGVWPESEMFNDKGFGPIPSRWKGGCESGELFNA 181

Query: 565  AKACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNVG 624
            +  C+RKLIGA+YF+ GL A +G   N T   +YLSPRD++GHGTHV+S  GGSF+PNV 
Sbjct: 182  SIHCNRKLIGAKYFVDGLVAEFG-VVNRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVS 241

Query: 625  YHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVLSLSLGPTF 684
            Y GL  GT RGGAP   +A+YK CW    G CS  D+LKA+D+AI+DGVD+LSLSLGP+ 
Sbjct: 242  YVGLGRGTARGGAPGVHIAVYKACW---SGYCSGADVLKAMDEAIHDGVDILSLSLGPSV 301

Query: 685  PSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVDRSFL 744
            P + +    +  ++GAFHAVAKGI VV AAGN+GP A +++N+ PW+LTVAA++ DRSF 
Sbjct: 302  PLFPET---EHTSVGAFHAVAKGIPVVIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFP 361

Query: 745  VAVTLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLN-DTWAAGNVVLCF 804
             A+TLGNN T +GQ ++ G    F  L YPE     D   CE LS N ++   G VVLCF
Sbjct: 362  TAITLGNNITILGQAIYGGPELGFVGLTYPESPLSGD---CEKLSANPNSTMEGKVVLCF 421

Query: 805  ASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQILNYIRS 864
            A+      ++    +V   GGLGLI+AKNPT ++ P    FP V I  ++G  IL YIRS
Sbjct: 422  AA---STPSNAAIAAVINAGGLGLIMAKNPTHSLTP-TRKFPWVSIDFELGTDILFYIRS 481

Query: 865  TRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPK 924
            TR+P VKI  S T  GQS ST VA FSSRGPNSV+PAILKPDIAAPGV ILAA+ P+   
Sbjct: 482  TRSPIVKIQASKTLFGQSVSTKVATFSSRGPNSVSPAILKPDIAAPGVNILAAISPNSSI 541

Query: 925  ERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGEVIFAEGR 984
                +A +SGTSMATP VS +V LLKSLH  WSP+AI+SAIVTTAW +DP GE IFA+G 
Sbjct: 542  NDGGFAMMSGTSMATPVVSGVVVLLKSLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGS 601

Query: 985  PMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITKRSITCPK 1044
              K A PFD+GGG++NP KA+ PGL+YDM   DY+ Y C++ +++ +IS +  +   CP 
Sbjct: 602  SRKLADPFDYGGGLINPEKAVKPGLIYDMTTDDYVMYMCSVDYSDISISRVLGKITVCPN 661

Query: 1045 RRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAISVKPRILKFN 1104
             +PS+LD+N+PSITIP+LR  + LTRTV NVG VNS YK  I PP GI ++V P  L F+
Sbjct: 662  PKPSVLDLNLPSITIPNLRGEVTLTRTVTNVGPVNSVYKVVIDPPTGINVAVTPAELVFD 721

Query: 1105 HKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            +   + SFTV VST H+V TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 722  YTTTKRSFTVRVSTTHKVNTGYYFGSLTWTDNMHNVAIPVSVRTQI 748

BLAST of Spg002375 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 1.7e-234
Identity = 410/770 (53.25%), Postives = 546/770 (70.91%), Query Frame = 0

Query: 386  RTTIFLVFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLASVLG 445
            RT+I++V +S    LN   + +  + AK  V+IVY+G + HD+ E + ++HH +L S+LG
Sbjct: 5    RTSIYVV-LSLVIFLNVQRSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLG 64

Query: 446  SQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDYLQL 505
            S+E + DS+VY+Y++GFSGFAAKLT +QA+ +++LP V+ VIP+  +K+ TTR+WDYL L
Sbjct: 65   SKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGL 124

Query: 506  SPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICESGELFNPAK 565
            S   P SLL ++ MG   IIG++DTG+WPESEVF D G GPVPS WKG CE+GE FN + 
Sbjct: 125  SAANPKSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSN 184

Query: 566  ACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNVGYH 625
             C++KLIGA+YFI G   A    FN+T   D++SPRD  GHGTHVS++AGGSFVPN+ Y 
Sbjct: 185  -CNKKLIGAKYFINGF-LAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYK 244

Query: 626  GLAVGTVRGGAPQSRLAMYKVCWRLNGG---VCSDIDILKAIDQAIYDGVDVLSLSLGPT 685
            GLA GTVRGGAP++ +AMYK CW L+      CS  DILKA+D+A++DGVDVLS+SLG +
Sbjct: 245  GLAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSS 304

Query: 686  FPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVDRSF 745
             P Y + D+ DGI  GAFHAV KGI VV + GNSGP + +V N  PW++TVAA+++DRSF
Sbjct: 305  VPLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSF 364

Query: 746  LVAVTLGNNWTTMGQGMFSGKLTRFHNLVYPE---VSDLDDPRACESLSLN-DTWAAGNV 805
               +TLGNN   +GQ M++G    F +LVYPE    S+      CE L  N +    G V
Sbjct: 365  ATPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKV 424

Query: 806  VLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQILN 865
            VLCF +  Y       +  VK+ GGLG+I+A++P  A++P +++FPCV +  ++G  IL 
Sbjct: 425  VLCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILL 484

Query: 866  YIRSTRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPP 925
            Y RS+ +P VKI PS T VGQ   T VA FSSRGPNS+APAILKPDIAAPGV+ILAA   
Sbjct: 485  YTRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAATTN 544

Query: 926  SDPKERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGEVIF 985
            +   ++  +  +SGTSMA P +S + ALLK+LH  WSPAAIRSAIVTTAW +DP+GE IF
Sbjct: 545  TTFSDQ-GFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIF 604

Query: 986  AEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITKRSI 1045
            AEG P K A PFD+GGG+VNP K+ +PGLVYDMG+ DY+ Y C++G+N ++IS +  ++ 
Sbjct: 605  AEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSISQLIGKTT 664

Query: 1046 TCPKRRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAISVKPRI 1105
             C   +PS+LD N+PSITIP+L+  + +TRTV NVG +NS Y+  + PP G  ++V P  
Sbjct: 665  VCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPET 724

Query: 1106 LKFNHKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            L FN   K++ F V VST H+  TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 725  LVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQI 770

BLAST of Spg002375 vs. ExPASy Swiss-Prot
Match: Q9SZY2 (Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 SV=2)

HSP 1 Score: 813.1 bits (2099), Expect = 3.9e-234
Identity = 413/772 (53.50%), Postives = 551/772 (71.37%), Query Frame = 0

Query: 383  KSSRTTIFLVFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLAS 442
            ++ RT+IF+V +S    LN     L  A A+S V+IVY+G + HD+ E + ++HH +L S
Sbjct: 2    RNHRTSIFVV-LSLVIILNGQSGFLPRAGAESKVHIVYLGEKQHDDPEFVTESHHRMLWS 61

Query: 443  VLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDY 502
            +LGS+E +  S+V+++++GFSGFAAKLT +QA+ +++LP V+ VIP+R +K  TTR+WDY
Sbjct: 62   LLGSKEEAHGSMVHSFRHGFSGFAAKLTESQAKKIADLPEVVHVIPDRFYKPATTRTWDY 121

Query: 503  LQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICESGELFN 562
            L LSP  P +LL ++ MG   IIG++D+G+WPESEVF D+ +GPVPS WKG CESGE FN
Sbjct: 122  LGLSPTNPKNLLNQTNMGEQMIIGIIDSGVWPESEVFNDNEIGPVPSHWKGGCESGEDFN 181

Query: 563  PAKACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNV 622
             +  C++KLIGA+YFI    A +   FN++   D++SPR Y+GHGTHV+++AGGS+VPN 
Sbjct: 182  -SSHCNKKLIGAKYFINAFLATH-ESFNSSESLDFISPRGYNGHGTHVATIAGGSYVPNT 241

Query: 623  GYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGV--CSDIDILKAIDQAIYDGVDVLSLSLG 682
             Y GLA GTVRGGAP++R+A+YK CW L+  +  CS  DILKA+D+AI+DGVDVLSLSLG
Sbjct: 242  SYKGLAGGTVRGGAPRARIAVYKTCWYLDLDIAACSSADILKAMDEAIHDGVDVLSLSLG 301

Query: 683  PTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVDR 742
               P Y + D+ DGIA GAFHAV KGI VV AAGN+GPAA +V N  PW+LTVAA+++DR
Sbjct: 302  -FEPLYPETDVRDGIATGAFHAVLKGITVVCAAGNAGPAAQTVGNTAPWILTVAATTLDR 361

Query: 743  SFLVAVTLGNNWTTMGQGMFSGKLTRFHNLVYPE---VSDLDDPRACESLSLN-DTWAAG 802
            SF+  +TLGNN   +GQ +++G    F +LVYPE    S+      CE L +N +   AG
Sbjct: 362  SFVTPMTLGNNKVILGQAIYTGTEVGFTSLVYPENPGNSNESFSGTCERLLINSNRTMAG 421

Query: 803  NVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQI 862
             VVLCF    Y  +    +  VK+ GGLG+I+A  P   + P +++FPCV +  ++G  I
Sbjct: 422  KVVLCFTESPYSISVTRAAHYVKRAGGLGVIIAGQPGNVLRPCLDDFPCVAVDYELGTYI 481

Query: 863  LNYIRSTRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAV 922
            L YIRS  +P VKI PS T +GQ   T VA FSSRGPN ++ AILKPDIAAPGV+ILAA 
Sbjct: 482  LFYIRSNGSPVVKIQPSRTLIGQPVGTKVASFSSRGPNPISAAILKPDIAAPGVSILAAT 541

Query: 923  PPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGEV 982
              +       + F+SGTSMATP +S IVALLK+LH  WSPAAIRSAIVTTAW +DP+GE 
Sbjct: 542  TTNTTFNDRGFIFLSGTSMATPTISGIVALLKALHPDWSPAAIRSAIVTTAWRTDPFGEQ 601

Query: 983  IFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITKR 1042
            IFAEG P K A PFD+GGG+VNP KA  PGLVYD+G+ DY+ Y C++G+N ++IS +  +
Sbjct: 602  IFAEGSPRKPADPFDYGGGLVNPEKATKPGLVYDLGLEDYVLYMCSVGYNETSISQLVGK 661

Query: 1043 SITCPKRRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAISVKP 1102
               C   +PS+LD N+PSITIP+L+  + L RT+ NVG + S Y+ A+ PP G  ++V P
Sbjct: 662  GTVCSYPKPSVLDFNLPSITIPNLKEEVTLPRTLTNVGPLESVYRVAVEPPLGTQVTVTP 721

Query: 1103 RILKFNHKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
              L FN   KR+SF V+VST H++ TGY FGSLTW D +H+V IP+SVRT +
Sbjct: 722  ETLVFNSTTKRVSFKVSVSTTHKINTGYYFGSLTWSDSLHNVTIPLSVRTQL 769

BLAST of Spg002375 vs. ExPASy Swiss-Prot
Match: Q9SZY3 (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 5.1e-234
Identity = 417/773 (53.95%), Postives = 542/773 (70.12%), Query Frame = 0

Query: 383  KSSRTTIFLVFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLAS 442
            KS RT IF+  I     LN L T +  A A+S V+IVY+G + HD+ E + ++HH +L S
Sbjct: 2    KSCRTLIFVAII-----LNGLSTFVAHAGAESKVHIVYLGEKQHDDPEFVTESHHRMLWS 61

Query: 443  VLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDY 502
            +LGS+E +  S+V++Y++GFSGFAAKLT +QA+ +++LP V+ V P+  +++ TTR+WDY
Sbjct: 62   LLGSKEDAHSSMVHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDY 121

Query: 503  LQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICESGELFN 562
            L LS   P +LL  + MG   IIG++D+G+WPESEVF D+G+GPVPS WKG C SGE F 
Sbjct: 122  LGLSVANPKNLLNDTNMGEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENFT 181

Query: 563  PAKACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNV 622
             ++ C++KLIGA+YFI G  A +   FN+T   D++SPRD SGHGTHV+++AGGS+VP++
Sbjct: 182  SSQ-CNKKLIGAKYFINGFLATH-ESFNSTESLDFISPRDRSGHGTHVATIAGGSYVPSI 241

Query: 623  GYHGLAVGTVRGGAPQSRLAMYKVCW---RLNGGVCSDIDILKAIDQAIYDGVDVLSLSL 682
             Y GLA GTVRGGAP++R+AMYK CW   R +   CS  DILKA+D+A++DGVDVLSLS+
Sbjct: 242  SYKGLAGGTVRGGAPRARIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSI 301

Query: 683  GPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVD 742
            G  FP + + D+   IA GAFHAV KGI VV + GNSGPAA +V N  PW+LTVAA+++D
Sbjct: 302  GYRFPYFPETDVRAVIATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLD 361

Query: 743  RSFLVAVTLGNNWTTMGQGMFSGKLTRFHNLVYPE---VSDLDDPRACESLSLNDTWA-A 802
            RSF   +TLGNN   +GQ M++G    F +LVYPE    S+      CE L  N     A
Sbjct: 362  RSFPTPITLGNNKLILGQAMYTGPELGFTSLVYPENPGNSNESFSGDCELLFFNSNHTMA 421

Query: 803  GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQ 862
            G VVLCF +             VK+ GGLG+IVA+NP   + P  ++FPCV +  ++G  
Sbjct: 422  GKVVLCFTTSTRYITVSSAVSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGTD 481

Query: 863  ILNYIRSTRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 922
            IL YIRST  P VKI PS T VGQ   T VA FSSRGPNS+ PAILKPDIAAPGV+ILAA
Sbjct: 482  ILLYIRSTGLPVVKIQPSKTLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILAA 541

Query: 923  VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGE 982
               +       + F+SGTSMA P +S +VALLK+LH  WSPAAIRSAIVTTAW +DP+GE
Sbjct: 542  TTTNKTFNDRGFIFLSGTSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPFGE 601

Query: 983  VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 1042
             IFAEG P K A PFD+GGG+VNP KA  PGLVYD+G+ DY+ Y C++G+N ++IS +  
Sbjct: 602  QIFAEGSPRKLADPFDYGGGLVNPEKAAKPGLVYDLGLEDYVLYMCSVGYNETSISQLVG 661

Query: 1043 RSITCPKRRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAISVK 1102
            +   C   +PS+LD N+PSITIP+L+  + LTRT+ NVG + S YK  I PP GI ++V 
Sbjct: 662  KGTVCSNPKPSVLDFNLPSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVT 721

Query: 1103 PRILKFNHKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            P  L FN   KR+SF V VST H++ TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 722  PETLLFNSTTKRVSFKVKVSTTHKINTGYFFGSLTWSDSLHNVTIPLSVRTQI 767

BLAST of Spg002375 vs. ExPASy TrEMBL
Match: A0A6J1E757 (subtilisin-like protease SBT3.9 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430558 PE=3 SV=1)

HSP 1 Score: 1395.6 bits (3611), Expect = 0.0e+00
Identity = 687/773 (88.87%), Postives = 732/773 (94.70%), Query Frame = 0

Query: 376  MSSFAHGKSSRTTIFLVFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKT 435
            M SFAHGKSS TTIFLV IS FTAL+ LCTL I   AKSNVYIVYMG +PHDNEELLVK 
Sbjct: 1    MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 436  HHGVLASVLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQ 495
            HHGVLASVLGSQE S DSLVY+YKYGFSGFAAKLTMAQAQM+SELPSV++VIPNRLHKMQ
Sbjct: 61   HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120

Query: 496  TTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGIC 555
            TTRSWDYLQLSPRFPNSLL+KSRMG+GAIIG+LDTGIWPESEVFCD+GLGPVPSRWKGIC
Sbjct: 121  TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 556  ESGELFNPAKACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAG 615
            ESGELF+PAKACSRKLIGARYF+KGLEAAYGHP NN+ FQDY SPRD SGHGTHVSSVA 
Sbjct: 181  ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240

Query: 616  GSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVL 675
            G FVPNV YHGLAVGTVRGGAP SR+AMYKVCW++NGGVC+DIDILKAIDQAIYDGVDVL
Sbjct: 241  GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 676  SLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAA 735
            SLSLGP+FPSYSDVDM +GIAIGAFHAVAKGI VVGAAGNSGPAAYSV+NIEPWLLTVAA
Sbjct: 301  SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 736  SSVDRSFLVAVTLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAA 795
            SSVD SFLVA+TLGNNWTTMGQGMFSGKLT+FHNLVYPEVSDLDD RACESLSLNDTWAA
Sbjct: 361  SSVDCSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420

Query: 796  GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQ 855
            GNVVLCFASDDY+D+TH+TS SVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLD+GMQ
Sbjct: 421  GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480

Query: 856  ILNYIRSTRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 915
            ILNYIRSTRNPQVKIG STTRVGQ  STTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481  ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 916  VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGE 975
            VPPSDPK+RNSYAFISGTSMATPHVSAIVALLK+LH+HWSPAAI+SAIVTTAW+SDPYG 
Sbjct: 541  VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 976  VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 1035
            VIFAEG+PM SA PFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCA G+NNSAISGITK
Sbjct: 601  VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITK 660

Query: 1036 RSITCPKRRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAISVK 1095
            RSI+CPKRRPSILDINVPSITIPSL H ++LTRTV NVG +NS+YKAAI  PPGI I+VK
Sbjct: 661  RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAVK 720

Query: 1096 PRILKFNHKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            PRILKFNHKMK ISFTVT+S+NHRVTTGYCFGSLTWLDG+HSVRIP+SVRTNI
Sbjct: 721  PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Spg002375 vs. ExPASy TrEMBL
Match: A0A6J1J508 (subtilisin-like protease SBT3.9 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482683 PE=3 SV=1)

HSP 1 Score: 1394.8 bits (3609), Expect = 0.0e+00
Identity = 686/773 (88.75%), Postives = 732/773 (94.70%), Query Frame = 0

Query: 376  MSSFAHGKSSRTTIFLVFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKT 435
            M SFAHGKSS TTIFLV IS FTAL+ LCTL I   AKSNVYIVYMG +PHDNEELLVK 
Sbjct: 1    MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 436  HHGVLASVLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQ 495
            HHGVLASVLGSQE S DSLVY+YKYGFSGFAAKLTMAQAQM+SELPSV++VIPNRLHK+Q
Sbjct: 61   HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKIQ 120

Query: 496  TTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGIC 555
            TTRSWDYLQLSPRFPNSLL+KSRMG+GAIIG+LDTGIWPESEVFCD+GLGPVPSRWKGIC
Sbjct: 121  TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 556  ESGELFNPAKACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAG 615
            ESGELF+PAKACSRKLIGARYF+KGLEAAYGHP NN+ FQDYLSPRD SGHGTHVSSVA 
Sbjct: 181  ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYLSPRDRSGHGTHVSSVAS 240

Query: 616  GSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVL 675
            G FVPNV YHGLAVGTVRGGAP SR+AMYKVCW++NGGVC+DIDILKAIDQAIYDGVDVL
Sbjct: 241  GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 676  SLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAA 735
            SLSLGP+FPSYSDVDM +GIAIGAFHAVAKGI VVGAAGNSGPAAYSV+NIEPWLLTVAA
Sbjct: 301  SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 736  SSVDRSFLVAVTLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAA 795
            SSVDRSFLVA+TLGNNWTTMGQGMFSGKLT+F NLVYPEVSDLDD RACESLSLNDTWA+
Sbjct: 361  SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFINLVYPEVSDLDDERACESLSLNDTWAS 420

Query: 796  GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQ 855
            GNVVLCFASDDY+DNTH+TSWSVKKVGGLGLIVAKNPTKAVEPFI+NFPCVQISLD+GMQ
Sbjct: 421  GNVVLCFASDDYNDNTHNTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDIGMQ 480

Query: 856  ILNYIRSTRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 915
            ILNYIRSTRNPQVKIG STTRVGQ  STTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481  ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 916  VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGE 975
            VPPSDPK RNSYAFISGTSMATPHVSAIVALLK+LH+HWSPAAI+SAIVTTAW+SDPYG 
Sbjct: 541  VPPSDPKNRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 976  VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 1035
            VIFAEGRPM SA PFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCA G+NNSAISGI+K
Sbjct: 601  VIFAEGRPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGISK 660

Query: 1036 RSITCPKRRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAISVK 1095
            RSI+CPKRRPSILDINVPSITIPSL HS++LTRTV NVG +NS+YKAAI  PPGI I+VK
Sbjct: 661  RSISCPKRRPSILDINVPSITIPSLTHSVSLTRTVTNVGAINSSYKAAIEVPPGITIAVK 720

Query: 1096 PRILKFNHKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            PRILKFNHK K ISFTVT+S+N RVTTGYCFGSLTWLDG+HSVRIP+SVRTNI
Sbjct: 721  PRILKFNHKTKSISFTVTISSNQRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Spg002375 vs. ExPASy TrEMBL
Match: A0A6J1CIU2 (subtilisin-like protease SBT3.9 OS=Momordica charantia OX=3673 GN=LOC111011560 PE=3 SV=1)

HSP 1 Score: 1394.8 bits (3609), Expect = 0.0e+00
Identity = 685/775 (88.39%), Postives = 732/775 (94.45%), Query Frame = 0

Query: 376  MSSFAHGKSSRTTIFLVFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKT 435
            MSSFAHGKSS TTI L+FISFFT L+DLCTLL+  DAKS+VY+VYMG RPHDNEELLVKT
Sbjct: 1    MSSFAHGKSSSTTILLLFISFFTLLSDLCTLLVPVDAKSHVYVVYMGKRPHDNEELLVKT 60

Query: 436  HHGVLASVLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQ 495
            HHGVLASVLGSQEA ADS+VYNYKYGFSGFAAKLTMAQAQ +SELP+VIKVIPNRLHKMQ
Sbjct: 61   HHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQKISELPTVIKVIPNRLHKMQ 120

Query: 496  TTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGIC 555
            TTRSWDYLQLSPR  NSLLQKSRMGNGAIIGLLDTGIWPESEVF D+GLGPVPSRWKGIC
Sbjct: 121  TTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTGIWPESEVFSDNGLGPVPSRWKGIC 180

Query: 556  ESGELFNPAKACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAG 615
            ESGELF+P KACSRKLIGARYFIKGLEAAYGHP+NN+GFQDYLSPRDY+GHGTHVSSVA 
Sbjct: 181  ESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGFQDYLSPRDYAGHGTHVSSVAS 240

Query: 616  GSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVL 675
            GSFV NV YHGLAVGTVRGGAPQSRLAMYKVCW+LNGGVCSD DILKAIDQAI+DGVDVL
Sbjct: 241  GSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGVCSDADILKAIDQAIHDGVDVL 300

Query: 676  SLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAA 735
            SLSLGPTFPSYSDVDMH+G+AIG FHA+ KGI VVGAAGN GPAAYSVANIEPWLLTVAA
Sbjct: 301  SLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAGNYGPAAYSVANIEPWLLTVAA 360

Query: 736  SSVDRSFLVAVTLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAA 795
            SSVDRSFLVA+TLGNNWTT+GQGMFSGKL RFHNLVYPEVSDL+D RACESLSLNDTWAA
Sbjct: 361  SSVDRSFLVAITLGNNWTTVGQGMFSGKLRRFHNLVYPEVSDLEDERACESLSLNDTWAA 420

Query: 796  GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQ 855
            GNVVLCFASDD++DNTH TS SVKKVG LGLIVAKNPTK VEPFIN+FPCVQ+SL++GM 
Sbjct: 421  GNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTKDVEPFINHFPCVQVSLEIGMH 480

Query: 856  ILNYIRSTRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 915
            ILNYIRSTRNPQV IGPS TR+GQS STTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481  ILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 916  VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGE 975
            VPPSDPK++NSYAFISGTSMATPHVSAIVALLKSLH+HWSPAAI+SAIVTTAWTSDPYGE
Sbjct: 541  VPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 600

Query: 976  VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 1035
             IFAEGRP+KSAGPFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCAMG+N+SAISGITK
Sbjct: 601  TIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMADYIRYFCAMGYNDSAISGITK 660

Query: 1036 RSITCPKRRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPP--PGIAIS 1095
            + I+CPKRRPSILD NVPSITIP L+HS+ +TRTV NVG VNSTYKAAI  P  PGIAI+
Sbjct: 661  KPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVGAVNSTYKAAIETPPGPGIAIA 720

Query: 1096 VKPRILKFNHKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            VKPR+LKFNHK K ISFTVTVS+NHRVTTGYCFGSLTWLDG+HSVRIPISVRT+I
Sbjct: 721  VKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWLDGVHSVRIPISVRTDI 775

BLAST of Spg002375 vs. ExPASy TrEMBL
Match: A0A6J1E3V3 (subtilisin-like protease SBT3.3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430558 PE=3 SV=1)

HSP 1 Score: 1206.0 bits (3119), Expect = 0.0e+00
Identity = 588/655 (89.77%), Postives = 627/655 (95.73%), Query Frame = 0

Query: 494  MQTTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKG 553
            MQTTRSWDYLQLSPRFPNSLL+KSRMG+GAIIG+LDTGIWPESEVFCD+GLGPVPSRWKG
Sbjct: 1    MQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKG 60

Query: 554  ICESGELFNPAKACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSV 613
            ICESGELF+PAKACSRKLIGARYF+KGLEAAYGHP NN+ FQDY SPRD SGHGTHVSSV
Sbjct: 61   ICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSV 120

Query: 614  AGGSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVD 673
            A G FVPNV YHGLAVGTVRGGAP SR+AMYKVCW++NGGVC+DIDILKAIDQAIYDGVD
Sbjct: 121  ASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVD 180

Query: 674  VLSLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTV 733
            VLSLSLGP+FPSYSDVDM +GIAIGAFHAVAKGI VVGAAGNSGPAAYSV+NIEPWLLTV
Sbjct: 181  VLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTV 240

Query: 734  AASSVDRSFLVAVTLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTW 793
            AASSVD SFLVA+TLGNNWTTMGQGMFSGKLT+FHNLVYPEVSDLDD RACESLSLNDTW
Sbjct: 241  AASSVDCSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTW 300

Query: 794  AAGNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVG 853
            AAGNVVLCFASDDY+D+TH+TS SVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLD+G
Sbjct: 301  AAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIG 360

Query: 854  MQILNYIRSTRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAIL 913
            MQILNYIRSTRNPQVKIG STTRVGQ  STTVAYFSSRGPNSVAPAILKPDIAAPGVAIL
Sbjct: 361  MQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAIL 420

Query: 914  AAVPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPY 973
            AAVPPSDPK+RNSYAFISGTSMATPHVSAIVALLK+LH+HWSPAAI+SAIVTTAW+SDPY
Sbjct: 421  AAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPY 480

Query: 974  GEVIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGI 1033
            G VIFAEG+PM SA PFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCA G+NNSAISGI
Sbjct: 481  GAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGI 540

Query: 1034 TKRSITCPKRRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAIS 1093
            TKRSI+CPKRRPSILDINVPSITIPSL H ++LTRTV NVG +NS+YKAAI  PPGI I+
Sbjct: 541  TKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA 600

Query: 1094 VKPRILKFNHKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            VKPRILKFNHKMK ISFTVT+S+NHRVTTGYCFGSLTWLDG+HSVRIP+SVRTNI
Sbjct: 601  VKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 655

BLAST of Spg002375 vs. ExPASy TrEMBL
Match: A0A6J1J1Z1 (subtilisin-like protease SBT3.3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482683 PE=3 SV=1)

HSP 1 Score: 1157.1 bits (2992), Expect = 0.0e+00
Identity = 564/630 (89.52%), Postives = 602/630 (95.56%), Query Frame = 0

Query: 519  MGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICESGELFNPAKACSRKLIGARYFI 578
            MG+GAIIG+LDTGIWPESEVFCD+GLGPVPSRWKGICESGELF+PAKACSRKLIGARYF+
Sbjct: 1    MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 60

Query: 579  KGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNVGYHGLAVGTVRGGAPQ 638
            KGLEAAYGHP NN+ FQDYLSPRD SGHGTHVSSVA G FVPNV YHGLAVGTVRGGAP 
Sbjct: 61   KGLEAAYGHPLNNSEFQDYLSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 120

Query: 639  SRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVLSLSLGPTFPSYSDVDMHDGIAIG 698
            SR+AMYKVCW++NGGVC+DIDILKAIDQAIYDGVDVLSLSLGP+FPSYSDVDM +GIAIG
Sbjct: 121  SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 180

Query: 699  AFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVDRSFLVAVTLGNNWTTMGQG 758
            AFHAVAKGI VVGAAGNSGPAAYSV+NIEPWLLTVAASSVDRSFLVA+TLGNNWTTMGQG
Sbjct: 181  AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 240

Query: 759  MFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAAGNVVLCFASDDYDDNTHDTSWSV 818
            MFSGKLT+F NLVYPEVSDLDD RACESLSLNDTWA+GNVVLCFASDDY+DNTH+TSWSV
Sbjct: 241  MFSGKLTKFINLVYPEVSDLDDERACESLSLNDTWASGNVVLCFASDDYNDNTHNTSWSV 300

Query: 819  KKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQILNYIRSTRNPQVKIGPSTTRVG 878
            KKVGGLGLIVAKNPTKAVEPFI+NFPCVQISLD+GMQILNYIRSTRNPQVKIG STTRVG
Sbjct: 301  KKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 360

Query: 879  QSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKERNSYAFISGTSMATP 938
            Q  STTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPK RNSYAFISGTSMATP
Sbjct: 361  QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKNRNSYAFISGTSMATP 420

Query: 939  HVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGEVIFAEGRPMKSAGPFDFGGGIVN 998
            HVSAIVALLK+LH+HWSPAAI+SAIVTTAW+SDPYG VIFAEGRPM SA PFDFGGGIVN
Sbjct: 421  HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGRPMMSARPFDFGGGIVN 480

Query: 999  PNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITKRSITCPKRRPSILDINVPSITIP 1058
            PNKA+DPGLVYDMGMADYI+YFCA G+NNSAISGI+KRSI+CPKRRPSILDINVPSITIP
Sbjct: 481  PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGISKRSISCPKRRPSILDINVPSITIP 540

Query: 1059 SLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAISVKPRILKFNHKMKRISFTVTVSTNH 1118
            SL HS++LTRTV NVG +NS+YKAAI  PPGI I+VKPRILKFNHK K ISFTVT+S+N 
Sbjct: 541  SLTHSVSLTRTVTNVGAINSSYKAAIEVPPGITIAVKPRILKFNHKTKSISFTVTISSNQ 600

Query: 1119 RVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            RVTTGYCFGSLTWLDG+HSVRIP+SVRTNI
Sbjct: 601  RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 630

BLAST of Spg002375 vs. TAIR 10
Match: AT1G32960.1 (Subtilase family protein )

HSP 1 Score: 822.0 bits (2122), Expect = 5.9e-238
Identity = 418/773 (54.08%), Postives = 548/773 (70.89%), Query Frame = 0

Query: 383  KSSRTTIFLVFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLAS 442
            +S R++I LV +S  T LN        ++ +S V+IVY+G + H + E + ++HH +LAS
Sbjct: 2    RSFRSSILLVLLSLITVLN---ATRARSETESKVHIVYLGEKKHHDPEFVTESHHQMLAS 61

Query: 443  VLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDY 502
            +LGS++ + DS+VY+Y++GFSGFAAKLT +QA+ +++LP V+ VIP+  H++ TTR+W+Y
Sbjct: 62   LLGSKKDADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELATTRTWEY 121

Query: 503  LQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICESGELFN 562
            L LS   P +LL  + MG+  IIG++DTG+WPESE F D+G+GP+P +WKG CESGE F 
Sbjct: 122  LGLSSANPKNLLNDTNMGDQVIIGVIDTGVWPESESFNDNGVGPIPRKWKGGCESGENFR 181

Query: 563  PAKACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNV 622
             +  C+RKLIGA+YFI G   A    FN T  +DY+S RD+ GHGTHV+S+AGGSFVPNV
Sbjct: 182  -STDCNRKLIGAKYFINGF-LAENKGFNTTESRDYISARDFDGHGTHVASIAGGSFVPNV 241

Query: 623  GYHGLAVGTVRGGAPQSRLAMYKVCW---RLNGGVCSDIDILKAIDQAIYDGVDVLSLSL 682
             Y GLA GT+RGGAP++R+AMYK CW    L G  CSD DI+KAID+AI+DGVDVLS+SL
Sbjct: 242  SYKGLAGGTLRGGAPRARIAMYKACWFHEELKGVTCSDSDIMKAIDEAIHDGVDVLSISL 301

Query: 683  GPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVD 742
                P  S+ D+ D  A G FHAVAKGI VV A GN GPAA +V NI PW+LTVAA+++D
Sbjct: 302  VGQIPLNSETDIRDEFATGLFHAVAKGIVVVCAGGNDGPAAQTVVNIAPWILTVAATTLD 361

Query: 743  RSFLVAVTLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPR---ACESLSLNDTWA-A 802
            RSF   +TLGNN   +GQ  ++G      +LVYPE +  ++      CESL+LN  +  A
Sbjct: 362  RSFPTPITLGNNKVILGQATYTGPELGLTSLVYPENARNNNETFSGVCESLNLNPNYTMA 421

Query: 803  GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQ 862
              VVLCF +   +      +  VK  GGLGLI+++NP   + P  ++FPCV +  ++G  
Sbjct: 422  MKVVLCFTASRTNAAISRAASFVKAAGGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTD 481

Query: 863  ILNYIRSTRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 922
            IL+YIRSTR+P VKI  S T  GQ   T V  FSSRGPNS++PAILKPDIAAPGV ILAA
Sbjct: 482  ILSYIRSTRSPVVKIQRSRTLSGQPVGTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAA 541

Query: 923  VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGE 982
              P+D      +A +SGTSMATP +S ++ALLK+LH  WSPAA RSAIVTTAW +DP+GE
Sbjct: 542  TSPNDTLNVGGFAMLSGTSMATPVISGVIALLKALHPEWSPAAFRSAIVTTAWRTDPFGE 601

Query: 983  VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 1042
             IFAEG   K + PFD+GGGIVNP KA +PGL+YDMG  DYI Y C+ G+N+S+IS +  
Sbjct: 602  QIFAEGSSRKVSDPFDYGGGIVNPEKAAEPGLIYDMGPQDYILYLCSAGYNDSSISQLVG 661

Query: 1043 RSITCPKRRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAISVK 1102
            +   C   +PS+LD+N+PSITIP+L+  + LTRTV NVGLV+S YK ++ PP G+ + V 
Sbjct: 662  QITVCSNPKPSVLDVNLPSITIPNLKDEVTLTRTVTNVGLVDSVYKVSVEPPLGVRVVVT 721

Query: 1103 PRILKFNHKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            P  L FN K   +SFTV VST H++ TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 722  PETLVFNSKTISVSFTVRVSTTHKINTGYYFGSLTWTDSVHNVVIPLSVRTQI 769

BLAST of Spg002375 vs. TAIR 10
Match: AT4G10520.1 (Subtilase family protein )

HSP 1 Score: 814.7 bits (2103), Expect = 9.5e-236
Identity = 426/766 (55.61%), Postives = 550/766 (71.80%), Query Frame = 0

Query: 385  SRTTIFL-VFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLASV 444
            S+T +FL +F+S    LN   + ++   A+S VY+VY+G + HDN E + ++HH +L S+
Sbjct: 2    SKTILFLALFLSI--VLNVQISFVV---AESKVYVVYLGEKEHDNPESVTESHHQMLWSL 61

Query: 445  LGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDYL 504
            LGS+EA  DS+VY+Y++GFSGFAAKLT +QAQ +SELP V++VIPN L++M TTR+WDYL
Sbjct: 62   LGSKEAVLDSIVYSYRHGFSGFAAKLTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYL 121

Query: 505  QLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICESGELFNP 564
             +SP   +SLLQK+ MG   I+G++D+G+WPESE+F D G GP+PSRWKG CESGELFN 
Sbjct: 122  GVSPGNSDSLLQKANMGYNVIVGVIDSGVWPESEMFNDKGFGPIPSRWKGGCESGELFNA 181

Query: 565  AKACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNVG 624
            +  C+RKLIGA+YF+ GL A +G   N T   +YLSPRD++GHGTHV+S  GGSF+PNV 
Sbjct: 182  SIHCNRKLIGAKYFVDGLVAEFG-VVNRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVS 241

Query: 625  YHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVLSLSLGPTF 684
            Y GL  GT RGGAP   +A+YK CW    G CS  D+LKA+D+AI+DGVD+LSLSLGP+ 
Sbjct: 242  YVGLGRGTARGGAPGVHIAVYKACW---SGYCSGADVLKAMDEAIHDGVDILSLSLGPSV 301

Query: 685  PSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVDRSFL 744
            P + +    +  ++GAFHAVAKGI VV AAGN+GP A +++N+ PW+LTVAA++ DRSF 
Sbjct: 302  PLFPET---EHTSVGAFHAVAKGIPVVIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFP 361

Query: 745  VAVTLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLN-DTWAAGNVVLCF 804
             A+TLGNN T +GQ ++ G    F  L YPE     D   CE LS N ++   G VVLCF
Sbjct: 362  TAITLGNNITILGQAIYGGPELGFVGLTYPESPLSGD---CEKLSANPNSTMEGKVVLCF 421

Query: 805  ASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQILNYIRS 864
            A+      ++    +V   GGLGLI+AKNPT ++ P    FP V I  ++G  IL YIRS
Sbjct: 422  AA---STPSNAAIAAVINAGGLGLIMAKNPTHSLTP-TRKFPWVSIDFELGTDILFYIRS 481

Query: 865  TRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPK 924
            TR+P VKI  S T  GQS ST VA FSSRGPNSV+PAILKPDIAAPGV ILAA+ P+   
Sbjct: 482  TRSPIVKIQASKTLFGQSVSTKVATFSSRGPNSVSPAILKPDIAAPGVNILAAISPNSSI 541

Query: 925  ERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGEVIFAEGR 984
                +A +SGTSMATP VS +V LLKSLH  WSP+AI+SAIVTTAW +DP GE IFA+G 
Sbjct: 542  NDGGFAMMSGTSMATPVVSGVVVLLKSLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGS 601

Query: 985  PMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITKRSITCPK 1044
              K A PFD+GGG++NP KA+ PGL+YDM   DY+ Y C++ +++ +IS +  +   CP 
Sbjct: 602  SRKLADPFDYGGGLINPEKAVKPGLIYDMTTDDYVMYMCSVDYSDISISRVLGKITVCPN 661

Query: 1045 RRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAISVKPRILKFN 1104
             +PS+LD+N+PSITIP+LR  + LTRTV NVG VNS YK  I PP GI ++V P  L F+
Sbjct: 662  PKPSVLDLNLPSITIPNLRGEVTLTRTVTNVGPVNSVYKVVIDPPTGINVAVTPAELVFD 721

Query: 1105 HKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            +   + SFTV VST H+V TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 722  YTTTKRSFTVRVSTTHKVNTGYYFGSLTWTDNMHNVAIPVSVRTQI 748

BLAST of Spg002375 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 814.3 bits (2102), Expect = 1.2e-235
Identity = 410/770 (53.25%), Postives = 546/770 (70.91%), Query Frame = 0

Query: 386  RTTIFLVFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLASVLG 445
            RT+I++V +S    LN   + +  + AK  V+IVY+G + HD+ E + ++HH +L S+LG
Sbjct: 5    RTSIYVV-LSLVIFLNVQRSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLG 64

Query: 446  SQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDYLQL 505
            S+E + DS+VY+Y++GFSGFAAKLT +QA+ +++LP V+ VIP+  +K+ TTR+WDYL L
Sbjct: 65   SKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGL 124

Query: 506  SPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICESGELFNPAK 565
            S   P SLL ++ MG   IIG++DTG+WPESEVF D G GPVPS WKG CE+GE FN + 
Sbjct: 125  SAANPKSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSN 184

Query: 566  ACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNVGYH 625
             C++KLIGA+YFI G   A    FN+T   D++SPRD  GHGTHVS++AGGSFVPN+ Y 
Sbjct: 185  -CNKKLIGAKYFINGF-LAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYK 244

Query: 626  GLAVGTVRGGAPQSRLAMYKVCWRLNGG---VCSDIDILKAIDQAIYDGVDVLSLSLGPT 685
            GLA GTVRGGAP++ +AMYK CW L+      CS  DILKA+D+A++DGVDVLS+SLG +
Sbjct: 245  GLAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSS 304

Query: 686  FPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVDRSF 745
             P Y + D+ DGI  GAFHAV KGI VV + GNSGP + +V N  PW++TVAA+++DRSF
Sbjct: 305  VPLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSF 364

Query: 746  LVAVTLGNNWTTMGQGMFSGKLTRFHNLVYPE---VSDLDDPRACESLSLN-DTWAAGNV 805
               +TLGNN   +GQ M++G    F +LVYPE    S+      CE L  N +    G V
Sbjct: 365  ATPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKV 424

Query: 806  VLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQILN 865
            VLCF +  Y       +  VK+ GGLG+I+A++P  A++P +++FPCV +  ++G  IL 
Sbjct: 425  VLCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILL 484

Query: 866  YIRSTRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPP 925
            Y RS+ +P VKI PS T VGQ   T VA FSSRGPNS+APAILKPDIAAPGV+ILAA   
Sbjct: 485  YTRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAATTN 544

Query: 926  SDPKERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGEVIF 985
            +   ++  +  +SGTSMA P +S + ALLK+LH  WSPAAIRSAIVTTAW +DP+GE IF
Sbjct: 545  TTFSDQ-GFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIF 604

Query: 986  AEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITKRSI 1045
            AEG P K A PFD+GGG+VNP K+ +PGLVYDMG+ DY+ Y C++G+N ++IS +  ++ 
Sbjct: 605  AEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSISQLIGKTT 664

Query: 1046 TCPKRRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAISVKPRI 1105
             C   +PS+LD N+PSITIP+L+  + +TRTV NVG +NS Y+  + PP G  ++V P  
Sbjct: 665  VCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPET 724

Query: 1106 LKFNHKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            L FN   K++ F V VST H+  TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 725  LVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQI 770

BLAST of Spg002375 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 814.3 bits (2102), Expect = 1.2e-235
Identity = 412/773 (53.30%), Postives = 543/773 (70.25%), Query Frame = 0

Query: 383  KSSRTTIFLVFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLAS 442
            KSS+ TIFL     F A          + AK  V+IVY+G + HD+ E + ++HH +L S
Sbjct: 26   KSSKETIFLTKERSFVA---------ESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWS 85

Query: 443  VLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDY 502
            +LGS+E + DS+VY+Y++GFSGFAAKLT +QA+ +++LP V+ VIP+  +K+ TTR+WDY
Sbjct: 86   LLGSKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDY 145

Query: 503  LQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICESGELFN 562
            L LS   P SLL ++ MG   IIG++DTG+WPESEVF D G GPVPS WKG CE+GE FN
Sbjct: 146  LGLSAANPKSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFN 205

Query: 563  PAKACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNV 622
             +  C++KLIGA+YFI G   A    FN+T   D++SPRD  GHGTHVS++AGGSFVPN+
Sbjct: 206  SSN-CNKKLIGAKYFINGF-LAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNI 265

Query: 623  GYHGLAVGTVRGGAPQSRLAMYKVCWRLNGG---VCSDIDILKAIDQAIYDGVDVLSLSL 682
             Y GLA GTVRGGAP++ +AMYK CW L+      CS  DILKA+D+A++DGVDVLS+SL
Sbjct: 266  SYKGLAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISL 325

Query: 683  GPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVD 742
            G + P Y + D+ DGI  GAFHAV KGI VV + GNSGP + +V N  PW++TVAA+++D
Sbjct: 326  GSSVPLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLD 385

Query: 743  RSFLVAVTLGNNWTTMGQGMFSGKLTRFHNLVYPE---VSDLDDPRACESLSLN-DTWAA 802
            RSF   +TLGNN   +GQ M++G    F +LVYPE    S+      CE L  N +    
Sbjct: 386  RSFATPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTME 445

Query: 803  GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQ 862
            G VVLCF +  Y       +  VK+ GGLG+I+A++P  A++P +++FPCV +  ++G  
Sbjct: 446  GKVVLCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTD 505

Query: 863  ILNYIRSTRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 922
            IL Y RS+ +P VKI PS T VGQ   T VA FSSRGPNS+APAILKPDIAAPGV+ILAA
Sbjct: 506  ILLYTRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAA 565

Query: 923  VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGE 982
               +   ++  +  +SGTSMA P +S + ALLK+LH  WSPAAIRSAIVTTAW +DP+GE
Sbjct: 566  TTNTTFSDQ-GFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGE 625

Query: 983  VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 1042
             IFAEG P K A PFD+GGG+VNP K+ +PGLVYDMG+ DY+ Y C++G+N ++IS +  
Sbjct: 626  QIFAEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSISQLIG 685

Query: 1043 RSITCPKRRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAISVK 1102
            ++  C   +PS+LD N+PSITIP+L+  + +TRTV NVG +NS Y+  + PP G  ++V 
Sbjct: 686  KTTVCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVT 745

Query: 1103 PRILKFNHKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            P  L FN   K++ F V VST H+  TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 746  PETLVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQI 786

BLAST of Spg002375 vs. TAIR 10
Match: AT4G10540.1 (Subtilase family protein )

HSP 1 Score: 812.8 bits (2098), Expect = 3.6e-235
Identity = 417/773 (53.95%), Postives = 542/773 (70.12%), Query Frame = 0

Query: 383  KSSRTTIFLVFISFFTALNDLCTLLITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLAS 442
            KS RT IF+  I     LN L T +  A A+S V+IVY+G + HD+ E + ++HH +L S
Sbjct: 2    KSCRTLIFVAII-----LNGLSTFVAHAGAESKVHIVYLGEKQHDDPEFVTESHHRMLWS 61

Query: 443  VLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDY 502
            +LGS+E +  S+V++Y++GFSGFAAKLT +QA+ +++LP V+ V P+  +++ TTR+WDY
Sbjct: 62   LLGSKEDAHSSMVHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDY 121

Query: 503  LQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICESGELFN 562
            L LS   P +LL  + MG   IIG++D+G+WPESEVF D+G+GPVPS WKG C SGE F 
Sbjct: 122  LGLSVANPKNLLNDTNMGEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENFT 181

Query: 563  PAKACSRKLIGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNV 622
             ++ C++KLIGA+YFI G  A +   FN+T   D++SPRD SGHGTHV+++AGGS+VP++
Sbjct: 182  SSQ-CNKKLIGAKYFINGFLATH-ESFNSTESLDFISPRDRSGHGTHVATIAGGSYVPSI 241

Query: 623  GYHGLAVGTVRGGAPQSRLAMYKVCW---RLNGGVCSDIDILKAIDQAIYDGVDVLSLSL 682
             Y GLA GTVRGGAP++R+AMYK CW   R +   CS  DILKA+D+A++DGVDVLSLS+
Sbjct: 242  SYKGLAGGTVRGGAPRARIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSI 301

Query: 683  GPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVD 742
            G  FP + + D+   IA GAFHAV KGI VV + GNSGPAA +V N  PW+LTVAA+++D
Sbjct: 302  GYRFPYFPETDVRAVIATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLD 361

Query: 743  RSFLVAVTLGNNWTTMGQGMFSGKLTRFHNLVYPE---VSDLDDPRACESLSLNDTWA-A 802
            RSF   +TLGNN   +GQ M++G    F +LVYPE    S+      CE L  N     A
Sbjct: 362  RSFPTPITLGNNKLILGQAMYTGPELGFTSLVYPENPGNSNESFSGDCELLFFNSNHTMA 421

Query: 803  GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDVGMQ 862
            G VVLCF +             VK+ GGLG+IVA+NP   + P  ++FPCV +  ++G  
Sbjct: 422  GKVVLCFTTSTRYITVSSAVSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGTD 481

Query: 863  ILNYIRSTRNPQVKIGPSTTRVGQSSSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 922
            IL YIRST  P VKI PS T VGQ   T VA FSSRGPNS+ PAILKPDIAAPGV+ILAA
Sbjct: 482  ILLYIRSTGLPVVKIQPSKTLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILAA 541

Query: 923  VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHTHWSPAAIRSAIVTTAWTSDPYGE 982
               +       + F+SGTSMA P +S +VALLK+LH  WSPAAIRSAIVTTAW +DP+GE
Sbjct: 542  TTTNKTFNDRGFIFLSGTSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPFGE 601

Query: 983  VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 1042
             IFAEG P K A PFD+GGG+VNP KA  PGLVYD+G+ DY+ Y C++G+N ++IS +  
Sbjct: 602  QIFAEGSPRKLADPFDYGGGLVNPEKAAKPGLVYDLGLEDYVLYMCSVGYNETSISQLVG 661

Query: 1043 RSITCPKRRPSILDINVPSITIPSLRHSINLTRTVMNVGLVNSTYKAAIVPPPGIAISVK 1102
            +   C   +PS+LD N+PSITIP+L+  + LTRT+ NVG + S YK  I PP GI ++V 
Sbjct: 662  KGTVCSNPKPSVLDFNLPSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVT 721

Query: 1103 PRILKFNHKMKRISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 1149
            P  L FN   KR+SF V VST H++ TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 722  PETLLFNSTTKRVSFKVKVSTTHKINTGYFFGSLTWSDSLHNVTIPLSVRTQI 767