Spg000074 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg000074
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	GDSL esterase/lipase
Location	scaffold6: 24212114 .. 24218318 (+)
RNA-Seq Expression	Spg000074
Synteny	Spg000074

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTAATTAGGATGATTGATCCGTATTGTATCGACGATCCATGTTTCATAAGGAGATATATTGGTGTTTTAATCAGTTAATTATGCTCGAATTGTCCTAGCTCAAATGTTAATTTAATTTATTTTTTTAAAAAAATAAAGGAATAATTATTTGACTCCGAACCATAAAAAAAAAAAAAAAAAAAAATAGAGAAGAAAATTCGGTAAACATGTTGGAGACAACTTAGTCCATAAAAGAATCATTAATAAATATTTTCAAAAAAAAAAAATCATTAATAAATATGAAAGATAATTCCCCAATGGGTCCCCAAGAAAGTCGCTCAACAGACTCTTCTTCTAGTTTTCACACTGAATTCCTCTCTCTCTCCCCCTATAAATTCTCTTCCATTCCACTCCATTAAGGAACGGAAAAAAAAGAAAAAAAACTCAAAGAAAAGAAAAATGGGGTCCCAAGTCTTATCGTTGAGCTTGGTCTTCCTCTTCTCCCTTTTGTTTAGCGTTTCAGATGGTGGCTTGGTTCCCGCAATGTACGTGTTCGGAGACTCGCTAGTAGATGTTGGAAATAACAACTACTTGAAGCTTTCACTCGCCAAAGCCAACTTTCCACACAACGGACTTGACTTTCCAACCAAAAACCCCACTGGAAGGTTCTCTAATGGCAAGAATGCTGCGGATTATGTCGGTAACTCCCACACAAACCCAAATATCCCTCCACTTTTCTTTCTCTCTTTTCTTTAACTACAACTACAAGAGTCTAATCGGACATATTTCTCAACAAAAACATAGTTTAACTACTTTTATGTACTTGCCATCAAGTGGTCGTGAGTTTGAATCCCAACCCCACATGATTGTAATAAAAATTAAATAAATAAGTTGGACCATCTCCCTGTCATTTTGCAACAACGTAAAATTGAAAAAGAGAACAAAAAGAAACGGCCATACAGATAGGATTCAAGTGGGGCCGATAAACTTCCATTTATTTGATTTTTTTCTTTTATAATCTTTTCCTCAAATAATAATAATAATAAATTTATATGAACTTAAATCCAAAAACAGTTGGTTCCATTTTAAATTCAATCCGATTGTTTTCACTGTTGTTCCGGTCTGTTCAACCAATTATTTTCGTAAATTTAAGGTCCTTTTAGAATATATTTTAATTTAAATATTTTTCAAGAATGCATTTTTTAAAAAATATTTTGTATAAAAATTGTTTAGAATTTAAACACACATATGTTTGGTTGCATTTCCTCACAAATGTTTTTATTCACAAGTTATTGTTTACTTAAGAAGTCTTTTTTAAAAAGCTTGTTTCAAGTGTTTCTCTCAAAATGGTTTATTTGGACTCCGTTTGATAACGATTTCGTTTTTTGTTTTTGGTTTTTGAAAATTATGTTTGTGTTCTCTCAATTTACTTGTCATGGTTTTCATTTTTGTTAAAAAGACATTTGAGATCTTAGCCAAATTCCAAAAACAAAAACAAATTTTTGAAAACTACTTCTTTTTGTTTTCAAAATTTGATTTGGTTTTTGAAAACATGGGAAGGTTGTAGATAACAAAACAAAGAAAGTCGTAGATGAAAGTAGTGTTTATAAGCTTAATTTTCAAAAACAAAAAAACAAAAAACGAAATGGTTATCAAACGAGGCCTTATCAATCTCATTTTCTAATAAATCGTTTTTAGTGGTTGCCAAACACTAGAAATTTTTTGAAAATAATTTATTTTCAAAATTAAACACTCACTTTAAACCAAACACACCCTTAATTTCTTTTAAGTCCATCAATTAAATTGGTTTGTTTATTTATTTATTTGTTAAATGTTGGTTTTAAATCATTTTCCAATGTTTTATGGAATAGATCAGTTGATTTATTATCGTTTATTAAAAAAGTTGAACCAACCTTTTTACATTTGTATTTAATCTATGTATATTTTTTGATTGGTTAAAATAGTTATGACTTTTCTAAAAATGAGAGGCTTCAAATCTTATTCCAACTAAACAAAAGTGCATGTCAATTACATGATACATCCCACTTTCTTCTATTAAATTCATAATGACATTGACAAACGAATTAAATAAGGTTTCCCATTTGTGTACCACTCTTCAATATTTTTATATTGGACTGCATATTATATCCCCATAAAACTTCTATTATGCTCTTTTTTTATTTTTTCATCCAATAAATGACATGACCGCATGCTTTTTAATATTCTTATTTTAAAATCTCTAATCAGTATTTTTATATTTGCCATCTTGAATGTTGGCAAATATTCTTTTTCCATATTATAAGCCCTTCTAAAAAGATCCAGATCTTGAATGTTGGCAAATACTTTTTCCTTATTATAAGGTCTCAATTGGATGAGATGTTTTTGTCATATGGCAACCATTTTACCTTTAAAAAAAATATTTATTTGAACTCACAAAAAAAGAAAAAGCATTCATTTGATATATTTAGATTCTTAAATTCTTCTTCTTCTTAAATTCTTCAACACGTAGACTTTTTTCATAAGTCAGGACGAAAAAAAAAGGGAAAAAAAGAAACAGAAGTTCTTCTTCTACAAGACCCAAGAAGAAAAAAAAAAAACAAAAACAAAACAAAAGTCAAATTTTCCAAGAAAATAGAATAAATGTAATTTTACTATTTTTTTTAATAGAGTTGGTATCTCTGGTGCGCATCCATTAAAAATAATAGAAAATTTATTTACATCATTTGTAAAATTCCTGAATCGCAAGGGTGTTTCAAGTCAAGTACTTTTATGCTTTTTTGTTATTTTTCCCTTTTGAAAAATTGGTTTATACATTAAAAATAAAACAAGACATATCAATTAAAAAATAATTGCTCTCCTTATTTATAATGTTCATTTCATTCCTAAAGTTGCTATGTCGTTTCATCTTTCACGGATGAAATCCCTAAAATGCATGAAAATTCTTATTTAGCAATGTTAAAGCATTGCAATGTAATTAAATTTACAAGAATACATTACTTAAGGTCCCGTTTGATAACCATTTTTGGTTTTTGGTTTTTGAAAATTAAGCCTTATTAAACACTACTTCCACCTACAAATTTTTTTGTTTTCCTAACACATTTTTTCCATGTTTTCAAAAACCAAGCCAAATTTTGAAAACTAAAAAAAGTAGTTTTCAAAAACTTGTTTTTGTTTTTGCATTTTGGCTTGAAATTCAAATGTGCCCTAGACAAATGTGAAAACCATGGTAAGAAAATGGGAAGAAAACAAGCATAATTTTTAAAAACCAAAAACCAAAAACCAAATGGTTATCAAACGGGGCCTAAACTTTTAGGTTTATGAGTGATTTAACATGTATTATAGTAGGGTCCTGTTTTCGAACTCCATTAATATCATTTTCTTTTTCATTAATATTGAATTCCACTTGTTTGAACCTTCTACACAAATGTCCAAACCCACAAGTGAGAGAGTGTTAAAACATTGATATAATTAAAGTTTCCATAACCCATTAGCTTAAATTTTAAAATGTTGTTTTACTTTAAATCATTAAATAGTAATTAAAAAAAATACTATTTGACTAATTTTCATTAATTTTCTATTAATTAATTAATAGAAAATTTGATTAGTTTTGAATGTAGTCTTATGACTCCCTTGGCCTTCCAACACGGTCTATGTGCACCCTCACGTACATCAAGTTAAAATGAACCTCTAATCTATGTTTTTTTTTCTTTTTGTTTTTGTTTTTGAAGGCTAACTTATATCTTATTAAAATGTATACCAGATAATTTTATAAAATAGATTAGTGATAAGAATCAAAATCAGTTTTTTTTTTTTTTTTAATACAAAATACATGGACTAAACAAAATATTTATATCGTTTCTTTTAAACAAAAAATTTATCAATTTATTTTAGGTGAGTATGATTACACAAACCAACAGTAATTTCATTTTCGTAAATATTACTAGATGTAGTAAGCATTGAATGTGAATAATAGTTTATATGAATATATATTTCCTTTTTTTCTTTTAGAATTTTGAATTTGACATAATAAATGAAAATTTGACCAGCTGAGAAGGTGGGCTTACCGACATCACCACCATATCTGTCTCTCATTTCCAAGTTTAGAAAGATCATAAATACGACACCGTTTACAACTGGCGTGAGCTTCGCTTCGGGAGGTGCCGGAATTTTTAACGAAACAAATAAACTTTTTGTAAGTACATCATTCCCTCGTCTAAATATATGGATAGGAAAATGTTAACTAACAAGTTTGGTCCAAAACTTTTAGGTGTGTTGAATATATTTTTGAATTTTAAAAATTATCACACACATCTTTAAACTTTTAATTATGTTTAATAATACGCTAAACTACAAAAATGTCAAATAAGTTCTTTAACTTTCAACTTTGTATTTAATAGATAGTCTGTAACATATTCGACATTTTTTTACATTAACAAATCTCTTAGAAACATAAAGATATAAAATTTTAAATTTTGTATCTAATAGGATTGTGATTTTTTTTTAAAAATAAATGTAATATAGTGTCTTATTAGACACAAAATTGAAATTTCGATGATTTATTAGACACAAAATAAAAAATCTAAAAATCTTTTTTTCCTTTTTAAGATAAATCTAGATTTATTAGACAGTTTACATAAATCGAAAGTCTATAGACTTATTATATATCTCTTAAAGTTTATGAATTTATTAGACACAAAATTAAAAATTCAAATACACTCTTGTAATTTAACAATAATAATAATAATAAAAAAAAAACAATGGAAGATAATTTATTTCCTTTCAATTTTTTTTCTTTGTCCTGCATCTTTTAGAAACAATCGGTGGCGATGGACCAACAAATTGAGTACTATTCGTCGGTGTACCAATATGTAGTTGGAGAGCTGGGATCATCTGCAGCACAAGTACACCTTTCCAAGTCTCTGTTCACCGTTGTGATTGGAAGCAATGACATCTTTGGTTATCACGAATCATCTGATCTGCGTAATAAGTACTCCCCACAGCAGTACGTGGATTTGATGGCCTCCACTCTCGAGTCACAGCTTAAGGTATAAATCATAATCTTTTTTTATGGTTTTGAGTGGCTGTGATTTCATAACTTTTTTTTTTTTTAAAAAAAAATTATTATGATAAAAAGAAAATAAGATTTACTTAGGCGATAAGATAAGATTTGAGTGGCTCTCGTTTCACTATGGTGTGAGACAGAGAAATCCAATAATTTCTTATTTTAAAAAGTTGTAAAGCTGATTTGAATTTGGGTTTAGAAAGTTTTTTAAATTGAGTGTACTATGTTCTATCATTTTTTAATTTAGTTTCTTCATTGGTGAATGCATATGTTTCTAAAACTTTATGAGCCTTTTTTTTTTATCACTATTTTGTTCTCAAAGATTAAGCCTGTGTCAATTTACATTTTATTGTAAAAATTAAAAAAAAAAACATCAGAAATTAAACCTTGATTAGTTAGTAATTAAGAATTGAAGTATGCAAGTTTTTACTGAATGGCAGCGATTATACGGGTATGGAGCACGAAAATACGTGGTGGCTGGGGTTGGATTAGTGGGGTGCACTCCATCACAAAGGAAGAGGAGCAAAACGGGCGAATGTGATGAGGAAGTAAACAATTGGGCGGCAGAGTACAACGTCGCCCTAAAATCAATGTTGCAGAGACTGAAAATGGAGCTCAAGGGCATTGCATTCTCCTACTTTGATGTATATGCAGTCATGTCCAACTTCGTTCGCACACCATCTTCTTACGGTGTAACCCGCTCTCCATTTCTGTTCAATTCAATTCAACATTATTCAACGCCATTTCTAAAGTGTATATGTTTCATCTTTTTGTGATTTCAGGATTCACAGAGATTAGAAGTGTTTGTTGTGGGCTTGGGAATCTGAACGCAAATGTTCCTTGCTTACCGATTGCAACTTTTTGCTCCAACAGAAACAACCACCTCTTTTGGGATCTCTATCATCCTACCCAACAAGCCAGTCGCATGTTTGCTAATTACATATTTGATGGTCCATTTACATACCCACTCAATGTCAAACAACTCATTTCACTTTGATCCTACGCACAAGAAAAAAAGAAAGAAAAGAATACCATCACACACATTATCTAATTAGAGTTTATCAGGAGCCCACTAGTGGGGAAAAAAAAAAAAAAGTCCCAAATTGTATTACTATATCACTTTTGATAGAGGAGAATAATGCTAAAATTCTGTTTGGTGATAA

mRNA sequence

ATGGGGTCCCAAGTCTTATCGTTGAGCTTGGTCTTCCTCTTCTCCCTTTTGTTTAGCGTTTCAGATGGTGGCTTGGTTCCCGCAATGTACGTGTTCGGAGACTCGCTAGTAGATGTTGGAAATAACAACTACTTGAAGCTTTCACTCGCCAAAGCCAACTTTCCACACAACGGACTTGACTTTCCAACCAAAAACCCCACTGGAAGGTTCTCTAATGGCAAGAATGCTGCGGATTATGTCGCTGAGAAGGTGGGCTTACCGACATCACCACCATATCTGTCTCTCATTTCCAAGTTTAGAAAGATCATAAATACGACACCGTTTACAACTGGCGTGAGCTTCGCTTCGGGAGGTGCCGGAATTTTTAACGAAACAAATAAACTTTTTAAACAATCGGTGGCGATGGACCAACAAATTGAGTACTATTCGTCGGTGTACCAATATGTAGTTGGAGAGCTGGGATCATCTGCAGCACAAGTACACCTTTCCAAGTCTCTGTTCACCGTTGTGATTGGAAGCAATGACATCTTTGGTTATCACGAATCATCTGATCTGCGTAATAAGTACTCCCCACAGCAGTACGTGGATTTGATGGCCTCCACTCTCGAGTCACAGCTTAAGCGATTATACGGGTATGGAGCACGAAAATACGTGGTGGCTGGGGTTGGATTAGTGGGGTGCACTCCATCACAAAGGAAGAGGAGCAAAACGGGCGAATGTGATGAGGAAGTAAACAATTGGGCGGCAGAGTACAACGTCGCCCTAAAATCAATGTTGCAGAGACTGAAAATGGAGCTCAAGGGCATTGCATTCTCCTACTTTGATGTATATGCAGTCATGTCCAACTTCGTTCGCACACCATCTTCTTACGGTGATTCACAGAGATTAGAAGTGTTTGTTGTGGGCTTGGGAATCTGA

Coding sequence (CDS)

ATGGGGTCCCAAGTCTTATCGTTGAGCTTGGTCTTCCTCTTCTCCCTTTTGTTTAGCGTTTCAGATGGTGGCTTGGTTCCCGCAATGTACGTGTTCGGAGACTCGCTAGTAGATGTTGGAAATAACAACTACTTGAAGCTTTCACTCGCCAAAGCCAACTTTCCACACAACGGACTTGACTTTCCAACCAAAAACCCCACTGGAAGGTTCTCTAATGGCAAGAATGCTGCGGATTATGTCGCTGAGAAGGTGGGCTTACCGACATCACCACCATATCTGTCTCTCATTTCCAAGTTTAGAAAGATCATAAATACGACACCGTTTACAACTGGCGTGAGCTTCGCTTCGGGAGGTGCCGGAATTTTTAACGAAACAAATAAACTTTTTAAACAATCGGTGGCGATGGACCAACAAATTGAGTACTATTCGTCGGTGTACCAATATGTAGTTGGAGAGCTGGGATCATCTGCAGCACAAGTACACCTTTCCAAGTCTCTGTTCACCGTTGTGATTGGAAGCAATGACATCTTTGGTTATCACGAATCATCTGATCTGCGTAATAAGTACTCCCCACAGCAGTACGTGGATTTGATGGCCTCCACTCTCGAGTCACAGCTTAAGCGATTATACGGGTATGGAGCACGAAAATACGTGGTGGCTGGGGTTGGATTAGTGGGGTGCACTCCATCACAAAGGAAGAGGAGCAAAACGGGCGAATGTGATGAGGAAGTAAACAATTGGGCGGCAGAGTACAACGTCGCCCTAAAATCAATGTTGCAGAGACTGAAAATGGAGCTCAAGGGCATTGCATTCTCCTACTTTGATGTATATGCAGTCATGTCCAACTTCGTTCGCACACCATCTTCTTACGGTGATTCACAGAGATTAGAAGTGTTTGTTGTGGGCTTGGGAATCTGA

Protein sequence

MGSQVLSLSLVFLFSLLFSVSDGGLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPHNGLDFPTKNPTGRFSNGKNAADYVAEKVGLPTSPPYLSLISKFRKIINTTPFTTGVSFASGGAGIFNETNKLFKQSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSKSLFTVVIGSNDIFGYHESSDLRNKYSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQRKRSKTGECDEEVNNWAAEYNVALKSMLQRLKMELKGIAFSYFDVYAVMSNFVRTPSSYGDSQRLEVFVVGLGI

Homology

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BLAST of Spg000074 vs. NCBI nr
Match: XP_022932219.1 (GDSL esterase/lipase At5g55050 [Cucurbita moschata])

HSP 1 Score: 485.7 bits (1249), Expect = 2.9e-133
Identity = 242/292 (82.88%), Postives = 266/292 (91.10%), Query Frame = 0


Query: 1   MGSQVLSLSLVFLFSLLFSVSDGGLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPHNGLD 60
           MGSQ+L   +  L S+  ++S+  LVPA+YVFGDSLVDVGNNN+LKLS+AKANFPHNG+D
Sbjct: 1   MGSQILFFFMFSLLSIFSTISESALVPAIYVFGDSLVDVGNNNHLKLSIAKANFPHNGID 60

Query: 61  FPTKNPTGRFSNGKNAADYVAEKVGLPTSPPYLSLISKFRKIINTTPFTTGVSFASGGAG 120
           FPTK PTGRFSNGKNAADY+AEKVGLPTSPPYLSLISKFRK+INT PF TGVSFASGGAG
Sbjct: 61  FPTKKPTGRFSNGKNAADYIAEKVGLPTSPPYLSLISKFRKVINTKPFKTGVSFASGGAG 120

Query: 121 IFNETNKLFKQSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSKSLFTVVIGSNDIFGYH 180
           IFNETN LFKQSVAMDQQIEYYS VYQ +V ELG SAAQ HLSKSLFTVVIGSNDIFGYH
Sbjct: 121 IFNETNNLFKQSVAMDQQIEYYSLVYQKLVVELGLSAAQAHLSKSLFTVVIGSNDIFGYH 180

Query: 181 ESSDLRNKYSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQRKRSKTGEC 240
           ESSDLR KYSPQQYV+ MASTL+SQLKRLYGYGARKYVV G+GLVGCTPSQRKRSKTGEC
Sbjct: 181 ESSDLRKKYSPQQYVESMASTLKSQLKRLYGYGARKYVVTGIGLVGCTPSQRKRSKTGEC 240

Query: 241 DEEVNNWAAEYNVALKSMLQRLKMEL-KGIAFSYFDVYAVMSNFVRTPSSYG 292
           DEEVN+WAAEYNVALKSMLQ LKMEL   I+F+YF+VYAVMS+F+R+PSS+G
Sbjct: 241 DEEVNSWAAEYNVALKSMLQTLKMELNNNISFTYFEVYAVMSDFIRSPSSFG 292

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BLAST of Spg000074 vs. NCBI nr
Match: KAG7015527.1 (GDSL esterase/lipase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 485.0 bits (1247), Expect = 4.9e-133
Identity = 245/294 (83.33%), Postives = 268/294 (91.16%), Query Frame = 0


Query: 1   MGSQVLSLSLVFLFSLL--FSVSDGGLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPHNG 60
           MGSQ+L     F+FSLL  FS+S+  LVPA+YVFGDSLVDVGNNN+LKLS+AKANFPHNG
Sbjct: 1   MGSQIL---FFFMFSLLSIFSISESALVPAIYVFGDSLVDVGNNNHLKLSIAKANFPHNG 60

Query: 61  LDFPTKNPTGRFSNGKNAADYVAEKVGLPTSPPYLSLISKFRKIINTTPFTTGVSFASGG 120
           +DFPTK PTGRFSNGKNAADY+AEKVGLPTSPPYLSLISKFRK+INT PF TGVSFASGG
Sbjct: 61  IDFPTKKPTGRFSNGKNAADYIAEKVGLPTSPPYLSLISKFRKVINTKPFKTGVSFASGG 120

Query: 121 AGIFNETNKLFKQSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSKSLFTVVIGSNDIFG 180
           AGIFNETN LFKQSVAMDQQIEYYS VYQ +V ELG SAAQ HLSKSLFTVVIGSNDIFG
Sbjct: 121 AGIFNETNNLFKQSVAMDQQIEYYSLVYQKLVVELGLSAAQAHLSKSLFTVVIGSNDIFG 180

Query: 181 YHESSDLRNKYSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQRKRSKTG 240
           YHESSDLR KYSPQQYV+ MASTL+SQLKRLYGYGARKYVV G+GL+GCTPSQRKRSKTG
Sbjct: 181 YHESSDLRKKYSPQQYVESMASTLKSQLKRLYGYGARKYVVTGIGLLGCTPSQRKRSKTG 240

Query: 241 ECDEEVNNWAAEYNVALKSMLQRLKMEL-KGIAFSYFDVYAVMSNFVRTPSSYG 292
           ECDEEVN WAA+YNVALKSMLQ LKMEL   I+F+YF+VYAVMS+F+R+PSS+G
Sbjct: 241 ECDEEVNGWAAQYNVALKSMLQTLKMELNNNISFTYFEVYAVMSDFIRSPSSFG 291

[Close]

BLAST of Spg000074 vs. NCBI nr
Match: XP_038903279.1 (GDSL esterase/lipase At5g55050 [Benincasa hispida])

HSP 1 Score: 484.2 bits (1245), Expect = 8.4e-133
Identity = 253/308 (82.14%), Postives = 273/308 (88.64%), Query Frame = 0


Query: 1   MGSQVLS--LSLVFL-FSL-LFSVSDGGLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPH 60
           MGSQ  S   SL FL FS+ LF VS+  LVPA+YVFGDSLVDVGNNN+LKLSLAKANFPH
Sbjct: 1   MGSQKPSSFSSLFFLIFSIYLFRVSNSALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPH 60

Query: 61  NGLDFPTKNPTGRFSNGKNAADYVAEKVGLPTSPPYLSLISKFRKIINTTPFTTGVSFAS 120
           NGLDFPTK PTGRFSNGKNAADYVAEKVGLPTSPPYLSLISKFRK+ NTTPF TGVSFAS
Sbjct: 61  NGLDFPTKKPTGRFSNGKNAADYVAEKVGLPTSPPYLSLISKFRKVTNTTPFKTGVSFAS 120

Query: 121 GGAGIFNETNKLFKQSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSKSLFTVVIGSNDI 180
           GGAGIFNETNKLFKQSVAMDQQIE+YS VY+ +VGELGS  A  HLSKSLFTVVIGSNDI
Sbjct: 121 GGAGIFNETNKLFKQSVAMDQQIEFYSLVYKTLVGELGSPGAAEHLSKSLFTVVIGSNDI 180

Query: 181 FGYHESSDLRNKYSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQRKRSK 240
           FGYHESSDLRNKYSPQQYVD MASTL+SQLKRLYGYGARKYVV G+G VGC PSQRKRSK
Sbjct: 181 FGYHESSDLRNKYSPQQYVDSMASTLKSQLKRLYGYGARKYVVGGIGPVGCAPSQRKRSK 240

Query: 241 TGECDEEVNNWAAEYNVALKSMLQRLKMELKGIAFSYFDVYAVMSNFVRTPSSYGDSQRL 300
            GECDEEVNNWAA YN+ALKSMLQ LKMELK I++SYFDVY VMSNF+R+PSS+G ++ +
Sbjct: 241 GGECDEEVNNWAAIYNIALKSMLQTLKMELKDISYSYFDVYQVMSNFLRSPSSFGFTE-I 300

Query: 301 EVFVVGLG 305
           +    GLG
Sbjct: 301 KSACCGLG 307

[Close]

BLAST of Spg000074 vs. NCBI nr
Match: XP_023552078.1 (GDSL esterase/lipase At5g55050 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 482.3 bits (1240), Expect = 3.2e-132
Identity = 245/294 (83.33%), Postives = 268/294 (91.16%), Query Frame = 0


Query: 1   MGSQVLSLSLVFLFSLL--FSVSDGGLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPHNG 60
           MGSQ+L     F+FSLL   S+S+  LVPA+YVFGDSLVDVGNNN+LKLS+AKANFPHNG
Sbjct: 1   MGSQLL---FFFIFSLLSISSISESALVPAVYVFGDSLVDVGNNNHLKLSIAKANFPHNG 60

Query: 61  LDFPTKNPTGRFSNGKNAADYVAEKVGLPTSPPYLSLISKFRKIINTTPFTTGVSFASGG 120
           +DFPTK PTGRFSNGKNAADY+AEKVGLPTSPPYLSLISKFRK+INT PF TGVSFASGG
Sbjct: 61  IDFPTKKPTGRFSNGKNAADYIAEKVGLPTSPPYLSLISKFRKVINTKPFKTGVSFASGG 120

Query: 121 AGIFNETNKLFKQSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSKSLFTVVIGSNDIFG 180
           AGIFNETN LFKQSVAMDQQIEYYS VYQ +V ELG SAAQ HLSKSLFTVVIGSNDIFG
Sbjct: 121 AGIFNETNDLFKQSVAMDQQIEYYSLVYQKLVVELGLSAAQAHLSKSLFTVVIGSNDIFG 180

Query: 181 YHESSDLRNKYSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQRKRSKTG 240
           YHESSDLR KYSPQQYV+ MASTL+SQLKRLYGYGARKYVV G+GLVGCTPSQRKRSKTG
Sbjct: 181 YHESSDLRKKYSPQQYVESMASTLKSQLKRLYGYGARKYVVTGIGLVGCTPSQRKRSKTG 240

Query: 241 ECDEEVNNWAAEYNVALKSMLQRLKMEL-KGIAFSYFDVYAVMSNFVRTPSSYG 292
           ECDEEVN+WAA+YNVALKSMLQ LKMEL   I+F+YF+VYAVMS+F+R+PSS+G
Sbjct: 241 ECDEEVNSWAAQYNVALKSMLQTLKMELNNNISFTYFEVYAVMSDFIRSPSSFG 291

[Close]

BLAST of Spg000074 vs. NCBI nr
Match: XP_008449153.1 (PREDICTED: GDSL esterase/lipase At5g55050 [Cucumis melo])

HSP 1 Score: 482.3 bits (1240), Expect = 3.2e-132
Identity = 247/308 (80.19%), Postives = 268/308 (87.01%), Query Frame = 0


Query: 1   MGSQVLSLSLVFL----FSLLFSVSDGGLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPH 60
           MGSQVL   L FL    FS+L  VS+  LVPA+YVFGDSLVDVGNNN+LKLSLAKANFPH
Sbjct: 1   MGSQVLLSFLFFLFFFFFSILMGVSNSSLVPAIYVFGDSLVDVGNNNHLKLSLAKANFPH 60

Query: 61  NGLDFPTKNPTGRFSNGKNAADYVAEKVGLPTSPPYLSLISKFRKIINTTPFTTGVSFAS 120
           NGLDFPTK PTGRFSNGKNAAD+VAE+VGL TSPPYLSLISKFRK +NTTPF TGVSFAS
Sbjct: 61  NGLDFPTKKPTGRFSNGKNAADFVAERVGLATSPPYLSLISKFRKTVNTTPFKTGVSFAS 120

Query: 121 GGAGIFNETNKLFKQSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSKSLFTVVIGSNDI 180
           GGAGIFNETN LFKQSVAMDQQIE YS VY+++VGELGSS A  HLSKSLFTVVIGSNDI
Sbjct: 121 GGAGIFNETNNLFKQSVAMDQQIELYSRVYKHLVGELGSSGAAAHLSKSLFTVVIGSNDI 180

Query: 181 FGYHESSDLRNKYSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQRKRSK 240
           FGYHESSDLR KYSPQQY+DLMASTL SQLKRL+GYGARKYVV G+GLVGC PSQRKRSK
Sbjct: 181 FGYHESSDLRQKYSPQQYLDLMASTLHSQLKRLHGYGARKYVVGGIGLVGCAPSQRKRSK 240

Query: 241 TGECDEEVNNWAAEYNVALKSMLQRLKMELKGIAFSYFDVYAVMSNFVRTPSSYGDSQRL 300
           TGECDEEVNNWAA YN ALKS L+ LKMEL  I+FS+FDVY VMSNFV +PSSYG ++ +
Sbjct: 241 TGECDEEVNNWAAIYNTALKSKLETLKMELNDISFSFFDVYQVMSNFVHSPSSYGFTE-I 300

Query: 301 EVFVVGLG 305
           +    GLG
Sbjct: 301 KSACCGLG 307

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BLAST of Spg000074 vs. ExPASy Swiss-Prot
Match: Q9FIA1 (GDSL esterase/lipase At5g55050 OS=Arabidopsis thaliana OX=3702 GN=At5g55050 PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 8.8e-77
Identity = 151/296 (51.01%), Postives = 202/296 (68.24%), Query Frame = 0


Query: 6   LSLSLVFLFSLLFSVSDG--------GLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPHN 65
           L++ L+FL  L F    G          +P +YVFGDSLVD GNNNYL +S++KAN+PHN
Sbjct: 9   LTIFLLFLGLLRFDSFPGLEAATGKLASIPGLYVFGDSLVDAGNNNYLPISISKANYPHN 68

Query: 66  GLDFPTKNPTGRFSNGKNAADYVAEKVGLPTSPPYLSLISKFRKIINTTPFTTGVSFASG 125
           G+DFP K PTGRF NGKNAAD +AEK GLP  PPYLSL    ++    +   TGV+FASG
Sbjct: 69  GVDFPNKKPTGRFCNGKNAADAIAEKFGLPLPPPYLSLRGLLKREKRKSAAVTGVNFASG 128

Query: 126 GAGIFNETNKLFKQSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSKSLFTVVIGSNDIF 185
           GAGIFN +++   Q++ + +Q+  + S+++ V+ +L  SAAQ+HLSKSLFTVVIGSND+F
Sbjct: 129 GAGIFNSSDQKLGQAIPLSKQVNNWLSIHEEVM-KLEPSAAQLHLSKSLFTVVIGSNDLF 188

Query: 186 GYHESSDLRNKYSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQR-KRSK 245
            Y  S  LR + +PQQY  LMA  L+ QLKR++  GAR++++ GV  +GCTP +R K S 
Sbjct: 189 DYFGSFKLRRQSNPQQYTQLMADKLKEQLKRIHDSGARRFLIIGVAQIGCTPGKRAKNST 248

Query: 246 TGECDEEVNNWAAEYNVALKSMLQRLKMELKG-IAFSYFDVYAVMSNFVRTPSSYG 292
             ECDE  N W + YN AL  MLQ+LK EL+G I ++YFD Y  + + +  P+ YG
Sbjct: 249 LHECDEGANMWCSLYNEALVKMLQQLKQELQGSITYTYFDNYKSLHDIISNPARYG 303

[Close]

BLAST of Spg000074 vs. ExPASy Swiss-Prot
Match: Q9FHQ1 (GDSL esterase/lipase At5g37690 OS=Arabidopsis thaliana OX=3702 GN=At5g37690 PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 5.8e-52
Identity = 103/264 (39.02%), Postives = 157/264 (59.47%), Query Frame = 0


Query: 30  YVFGDSLVDVGNNNYLKLSLAKANFPHNGLDFPTKNPTGRFSNGKNAADYVAEKVGLPTS 89
           Y+FGDSL +VGNNN+L+ SLA+A+FP+ G+DF     TGRF+NG+   D ++ K+G+ + 
Sbjct: 29  YIFGDSLTEVGNNNFLQYSLARADFPYYGVDFSGGKATGRFTNGRTIGDIISTKLGILSP 88

Query: 90  PPYLSLISKFRKIINTTPFTTGVSFASGGAGIFNETNKLFKQSVAMDQQIEYYSSVYQYV 149
           PPYLSL        N   F +G+++ASGGAGI NET   F Q +  + QI  +    + +
Sbjct: 89  PPYLSLSQ------NDDAFLSGINYASGGAGILNETGIYFIQRLTFNDQINCFKKTKEVI 148

Query: 150 VGELGSSAAQVHLSKSLFTVVIGSNDIFGYHESSDLRN--KYSPQQYVDLMASTLESQLK 209
             ++G  AA  H++ +++ + +GSND         + +  +Y+  ++V+L+ STL +QL 
Sbjct: 149 RAKIGDGAANKHVNDAMYFIGLGSNDYVNNFLQPFMADGQQYTHDEFVELLTSTLHNQLT 208

Query: 210 RLYGYGARKYVVAGVGLVGCTPSQRKRSKTGECDEEVNNWAAEYNVALKSMLQRLKMELK 269
            +Y  GARK +  G+G +GC PSQR +SKT  C   VN W  E+N   K +L  L   L 
Sbjct: 209 TIYKLGARKVIFHGLGPLGCIPSQRVKSKTRMCLNRVNEWVLEFNSRTKKLLIDLNKRLP 268

Query: 270 GIAFSYFDVYAVMSNFVRTPSSYG 292
           G  FS+ D Y  + + +  P+ YG
Sbjct: 269 GAKFSFADTYPAVLDLINNPTHYG 286

[Close]

BLAST of Spg000074 vs. ExPASy Swiss-Prot
Match: O23470 (GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3 SV=2)

HSP 1 Score: 188.3 bits (477), Expect = 1.2e-46
Identity = 113/295 (38.31%), Postives = 174/295 (58.98%), Query Frame = 0


Query: 4   QVLSLSLVFLFSLLFSVSDGGLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPHNGLDFPT 63
           Q++ LS++F   +  +   G  +PA +VFGDSLVD GNNNYL  +L+KAN+  NG+DF  
Sbjct: 9   QIIVLSVLFFSEVCLA---GKKIPANFVFGDSLVDAGNNNYL-ATLSKANYVPNGIDF-- 68

Query: 64  KNPTGRFSNGKNAADYVAEKVGL-PTSPPYLSLISKFRKIINTTPFTTGVSFASGGAGIF 123
            +PTGRF+NG+   D V + +G    +PPYL+  +    I+N      GV++ASGG+GI 
Sbjct: 69  GSPTGRFTNGRTIVDIVYQALGSDELTPPYLAPTTSGSLILN------GVNYASGGSGIL 128

Query: 124 NETNKLFKQSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSKSLFTVVIGSNDIFGYHES 183
           N T KLF + + +D Q++ +++  Q ++  +G S A      ++F+V  GSND+   + +
Sbjct: 129 NSTGKLFGERINVDAQLDNFATTRQDIISWIGESEAAKLFRSAIFSVTTGSNDLINNYFT 188

Query: 184 ---SDLRNK-YSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQRKRSKT- 243
              S L+ K  +P+ +VD M S    QL RLY  GARK VV  +G +GC P +R+     
Sbjct: 189 PVISTLQRKVVAPEVFVDTMISKFRLQLTRLYQLGARKIVVINIGPIGCIPFERESDPAA 248

Query: 244 -GECDEEVNNWAAEYNVALKSMLQRLKMELKGIAFSYFDVYAVMSNFVRTPSSYG 292
              C  E N  A  YN+ LK++++ L   L+G  F Y DV+ ++ + ++  SSYG
Sbjct: 249 GNNCLAEPNEVAQMYNLKLKTLVEELNKNLQGSRFVYGDVFRIVDDIIQNYSSYG 291

[Close]

BLAST of Spg000074 vs. ExPASy Swiss-Prot
Match: Q9M2R9 (GDSL esterase/lipase At3g50400 OS=Arabidopsis thaliana OX=3702 GN=At3g50400 PE=2 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 5.2e-45
Identity = 116/289 (40.14%), Postives = 169/289 (58.48%), Query Frame = 0


Query: 14  FSLLFSVSDGGLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPHNGLDF-PTK-NPTGRFS 73
           FS + S  D   + A +VFGDSLVD GNNNYL+ +L++AN P NG+DF P++ NPTGRF+
Sbjct: 20  FSRVASAGDQRALAASFVFGDSLVDAGNNNYLQ-TLSRANSPPNGIDFKPSRGNPTGRFT 79

Query: 74  NGKNAADYVAEKVGLPT-SPPYLSLISKFRKIINTTPFTTGVSFASGGAGIFNETNKLFK 133
           NG+  AD V EK+G  + + PYL+  +    ++N      GV++ASGG GI N T  +F 
Sbjct: 80  NGRTIADIVGEKLGQQSYAVPYLAPNASGEALLN------GVNYASGGGGILNATGSVFV 139

Query: 134 QSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSK-SLFTVVIGSNDIFGYH----ESSDL 193
             + MD Q++Y+++  +     LG   A+ ++ K SLF+VVIGSND    +     ++  
Sbjct: 140 NRLGMDIQVDYFTNTRKQFDKLLGQDKARDYIRKRSLFSVVIGSNDFLNNYLVPFVAAQA 199

Query: 194 RNKYSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQRKRSKTG--ECDEE 253
           R   +P+ +VD M S L +QLKRLY   ARK+VV  V  +GC P Q+  ++    +C + 
Sbjct: 200 RLTQTPETFVDDMISHLRNQLKRLYDMDARKFVVGNVAPIGCIPYQKSINQLNDKQCVDL 259

Query: 254 VNNWAAEYNVALKSMLQ-RLKMELKGIAFSYFDVYAVMSNFVRTPSSYG 292
            N  A +YN  LK +L   LK  LK   F Y +VY +  + +     YG
Sbjct: 260 ANKLAIQYNARLKDLLTVELKDSLKDAHFVYANVYDLFMDLIVNFKDYG 301

[Close]

BLAST of Spg000074 vs. ExPASy Swiss-Prot
Match: Q9CA68 (GDSL esterase/lipase At1g74460 OS=Arabidopsis thaliana OX=3702 GN=At1g74460 PE=2 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 7.6e-44
Identity = 99/283 (34.98%), Postives = 152/283 (53.71%), Query Frame = 0


Query: 11  VFLFSLLFSVSDGGLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPHNGLDFPTKNPTGRF 70
           +F+  ++ +++        ++FGDSL DVGNN  L  SLA AN P  G+DF    P GRF
Sbjct: 6   IFVLFIVLAINGYDCKIVQFIFGDSLSDVGNNKNLPRSLATANLPFYGIDFGNGLPNGRF 65

Query: 71  SNGKNAADYVAEKVGLPTSPPYLSLISKFRKIINTTPFTTGVSFASGGAGIFNETNKLFK 130
           +NG+  +D + +K+GLP    +L        I+       GV++ASGG GI NET   F 
Sbjct: 66  TNGRTVSDIIGDKIGLPRPVAFLDPSMNEDVILE-----NGVNYASGGGGILNETGGYFI 125

Query: 131 QSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSKSLFTVVIGSNDIFGYHESSDLRN--K 190
           Q  ++ +QIE +      VV ++G   A      + + V +GSND    +      +  K
Sbjct: 126 QRFSLWKQIELFQGTQDVVVAKIGKKEADKFFQDARYVVALGSNDFINNYLMPVYSDSWK 185

Query: 191 YSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQRKRSKTGECDEEVNNWA 250
           Y+ Q +VD +  TLESQLK L+  GARK +V G+G +GC P QR  S  G C  + +N A
Sbjct: 186 YNDQTFVDYLMETLESQLKVLHSLGARKLMVFGLGPMGCIPLQRALSLDGNCQNKASNLA 245

Query: 251 AEYNVALKSMLQRLKMELKGIAFSYFDVYAVMSNFVRTPSSYG 292
             +N A  +ML  L+ +L   ++ + + Y ++++ +  P  YG
Sbjct: 246 KRFNKAATTMLLDLETKLPNASYRFGEAYDLVNDVITNPKKYG 283

[Close]

BLAST of Spg000074 vs. ExPASy TrEMBL
Match: A0A6J1EWE7 (GDSL esterase/lipase At5g55050 OS=Cucurbita moschata OX=3662 GN=LOC111438588 PE=3 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.4e-133
Identity = 242/292 (82.88%), Postives = 266/292 (91.10%), Query Frame = 0


Query: 1   MGSQVLSLSLVFLFSLLFSVSDGGLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPHNGLD 60
           MGSQ+L   +  L S+  ++S+  LVPA+YVFGDSLVDVGNNN+LKLS+AKANFPHNG+D
Sbjct: 1   MGSQILFFFMFSLLSIFSTISESALVPAIYVFGDSLVDVGNNNHLKLSIAKANFPHNGID 60

Query: 61  FPTKNPTGRFSNGKNAADYVAEKVGLPTSPPYLSLISKFRKIINTTPFTTGVSFASGGAG 120
           FPTK PTGRFSNGKNAADY+AEKVGLPTSPPYLSLISKFRK+INT PF TGVSFASGGAG
Sbjct: 61  FPTKKPTGRFSNGKNAADYIAEKVGLPTSPPYLSLISKFRKVINTKPFKTGVSFASGGAG 120

Query: 121 IFNETNKLFKQSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSKSLFTVVIGSNDIFGYH 180
           IFNETN LFKQSVAMDQQIEYYS VYQ +V ELG SAAQ HLSKSLFTVVIGSNDIFGYH
Sbjct: 121 IFNETNNLFKQSVAMDQQIEYYSLVYQKLVVELGLSAAQAHLSKSLFTVVIGSNDIFGYH 180

Query: 181 ESSDLRNKYSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQRKRSKTGEC 240
           ESSDLR KYSPQQYV+ MASTL+SQLKRLYGYGARKYVV G+GLVGCTPSQRKRSKTGEC
Sbjct: 181 ESSDLRKKYSPQQYVESMASTLKSQLKRLYGYGARKYVVTGIGLVGCTPSQRKRSKTGEC 240

Query: 241 DEEVNNWAAEYNVALKSMLQRLKMEL-KGIAFSYFDVYAVMSNFVRTPSSYG 292
           DEEVN+WAAEYNVALKSMLQ LKMEL   I+F+YF+VYAVMS+F+R+PSS+G
Sbjct: 241 DEEVNSWAAEYNVALKSMLQTLKMELNNNISFTYFEVYAVMSDFIRSPSSFG 292

[Close]

BLAST of Spg000074 vs. ExPASy TrEMBL
Match: A0A1S3BM17 (GDSL esterase/lipase At5g55050 OS=Cucumis melo OX=3656 GN=LOC103491100 PE=3 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.5e-132
Identity = 247/308 (80.19%), Postives = 268/308 (87.01%), Query Frame = 0


Query: 1   MGSQVLSLSLVFL----FSLLFSVSDGGLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPH 60
           MGSQVL   L FL    FS+L  VS+  LVPA+YVFGDSLVDVGNNN+LKLSLAKANFPH
Sbjct: 1   MGSQVLLSFLFFLFFFFFSILMGVSNSSLVPAIYVFGDSLVDVGNNNHLKLSLAKANFPH 60

Query: 61  NGLDFPTKNPTGRFSNGKNAADYVAEKVGLPTSPPYLSLISKFRKIINTTPFTTGVSFAS 120
           NGLDFPTK PTGRFSNGKNAAD+VAE+VGL TSPPYLSLISKFRK +NTTPF TGVSFAS
Sbjct: 61  NGLDFPTKKPTGRFSNGKNAADFVAERVGLATSPPYLSLISKFRKTVNTTPFKTGVSFAS 120

Query: 121 GGAGIFNETNKLFKQSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSKSLFTVVIGSNDI 180
           GGAGIFNETN LFKQSVAMDQQIE YS VY+++VGELGSS A  HLSKSLFTVVIGSNDI
Sbjct: 121 GGAGIFNETNNLFKQSVAMDQQIELYSRVYKHLVGELGSSGAAAHLSKSLFTVVIGSNDI 180

Query: 181 FGYHESSDLRNKYSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQRKRSK 240
           FGYHESSDLR KYSPQQY+DLMASTL SQLKRL+GYGARKYVV G+GLVGC PSQRKRSK
Sbjct: 181 FGYHESSDLRQKYSPQQYLDLMASTLHSQLKRLHGYGARKYVVGGIGLVGCAPSQRKRSK 240

Query: 241 TGECDEEVNNWAAEYNVALKSMLQRLKMELKGIAFSYFDVYAVMSNFVRTPSSYGDSQRL 300
           TGECDEEVNNWAA YN ALKS L+ LKMEL  I+FS+FDVY VMSNFV +PSSYG ++ +
Sbjct: 241 TGECDEEVNNWAAIYNTALKSKLETLKMELNDISFSFFDVYQVMSNFVHSPSSYGFTE-I 300

Query: 301 EVFVVGLG 305
           +    GLG
Sbjct: 301 KSACCGLG 307

[Close]

BLAST of Spg000074 vs. ExPASy TrEMBL
Match: A0A6J1DE65 (GDSL esterase/lipase At5g55050-like OS=Momordica charantia OX=3673 GN=LOC111019250 PE=3 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.6e-132
Identity = 243/293 (82.94%), Postives = 260/293 (88.74%), Query Frame = 0


Query: 1   MGSQVLSLSLVFL-FSLLFSVSD-GGLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPHNG 60
           MGS+      +FL  S  F V + G LVPA+YVFGDSLVDVGNNN+LKLSLAKANFPHNG
Sbjct: 1   MGSRAFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNG 60

Query: 61  LDFPTKNPTGRFSNGKNAADYVAEKVGLPTSPPYLSLISKFRKIINTTPFTTGVSFASGG 120
           +DFPT  PTGRFSNGKNAADY+AEKVGLPTSPPYLSL+SKFRKI NTTPF TGVSFASGG
Sbjct: 61  IDFPTTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGG 120

Query: 121 AGIFNETNKLFKQSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSKSLFTVVIGSNDIFG 180
           AGIFNETN LFKQSVAM QQI++YSSVYQ++V ELGSSAAQVHLSKSLFTVVIGSNDIFG
Sbjct: 121 AGIFNETNNLFKQSVAMGQQIDFYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFG 180

Query: 181 YHESSDLRNKYSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQRKRSKTG 240
           YHESSDLR KYSPQQYVDLMASTLESQLKRLY YGARKYV+ GVGLVGCTPSQRKRS+TG
Sbjct: 181 YHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRSRTG 240

Query: 241 ECDEEVNNWAAEYNVALKSMLQRLKMELKGIAFSYFDVYAVMSNFVRTPSSYG 292
           ECD EVN+W   YN  LK+ML RLKMEL GIAFSYFD YAVMSNF+RTPSSYG
Sbjct: 241 ECDAEVNSWVEHYNFGLKAMLGRLKMELNGIAFSYFDAYAVMSNFIRTPSSYG 293

[Close]

BLAST of Spg000074 vs. ExPASy TrEMBL
Match: A0A6J1HMQ2 (GDSL esterase/lipase At5g55050 OS=Cucurbita maxima OX=3661 GN=LOC111465054 PE=3 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 4.5e-132
Identity = 240/292 (82.19%), Postives = 265/292 (90.75%), Query Frame = 0


Query: 1   MGSQVLSLSLVFLFSLLFSVSDGGLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPHNGLD 60
           MGSQ+L   +  L S+  ++S+  LVPA+YVFGDSLVDVGNNN+LKLS+AKANFPHNG+D
Sbjct: 1   MGSQLLFCFIFSLLSIFSTISESALVPAIYVFGDSLVDVGNNNHLKLSIAKANFPHNGID 60

Query: 61  FPTKNPTGRFSNGKNAADYVAEKVGLPTSPPYLSLISKFRKIINTTPFTTGVSFASGGAG 120
           FPTK PTGRFSNGKNAADY+AEKVGLPTSPPYLSLISKFRK+INT PF TGVSFASGGAG
Sbjct: 61  FPTKKPTGRFSNGKNAADYIAEKVGLPTSPPYLSLISKFRKVINTKPFKTGVSFASGGAG 120

Query: 121 IFNETNKLFKQSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSKSLFTVVIGSNDIFGYH 180
           IFNETN LFKQSVA+DQQIEYYS VYQ +V ELG SAA+ HLSKSLFTVVIGSNDIFGYH
Sbjct: 121 IFNETNNLFKQSVAIDQQIEYYSLVYQNLVVELGLSAAEAHLSKSLFTVVIGSNDIFGYH 180

Query: 181 ESSDLRNKYSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQRKRSKTGEC 240
           ESSDLR KYSPQQYV+ MASTL+SQLKRLYGYGARKYVV G+GLVGCTPSQRKRSKTGEC
Sbjct: 181 ESSDLRKKYSPQQYVESMASTLKSQLKRLYGYGARKYVVTGIGLVGCTPSQRKRSKTGEC 240

Query: 241 DEEVNNWAAEYNVALKSMLQRLKMEL-KGIAFSYFDVYAVMSNFVRTPSSYG 292
           D EVN+WAA+YNVALKSMLQ LKMEL   I+FSYF+VYA+MS+FVR+PSSYG
Sbjct: 241 DVEVNSWAAQYNVALKSMLQTLKMELNNNISFSYFEVYAIMSDFVRSPSSYG 292

[Close]

BLAST of Spg000074 vs. ExPASy TrEMBL
Match: A0A0A0L695 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G075990 PE=3 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 3.6e-129
Identity = 240/305 (78.69%), Postives = 264/305 (86.56%), Query Frame = 0


Query: 1   MGSQ-VLSLSLVFLFSLLFSVSDGGLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPHNGL 60
           MGSQ +LSL L+  FS L  VS+  LVPA+YVFGDSLVDVGNNN+LKLSLAKANFPHNGL
Sbjct: 1   MGSQLLLSLLLLLFFSSLIRVSNSSLVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNGL 60

Query: 61  DFPTKNPTGRFSNGKNAADYVAEKVGLPTSPPYLSLISKFRKIINTTPFTTGVSFASGGA 120
           DFPTK PTGRFSNGKNAAD+VAE+VGL TSPPYLSLISKFRK +NT PF TGVSFASGGA
Sbjct: 61  DFPTKKPTGRFSNGKNAADFVAERVGLATSPPYLSLISKFRKTVNTAPFKTGVSFASGGA 120

Query: 121 GIFNETNKLFKQSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSKSLFTVVIGSNDIFGY 180
           GIFNETN LFKQSVAM+QQIE YS VY  +VGELGSS A  HLSKSLFT+VIGSNDIFGY
Sbjct: 121 GIFNETNNLFKQSVAMEQQIELYSRVYTNLVGELGSSGAAAHLSKSLFTIVIGSNDIFGY 180

Query: 181 HESSDLRNKYSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQRKRSKTGE 240
           HESSDLR KYSPQQY+DLMASTL SQLKRL+GYGARKYVV G+GLVGC PSQRKRS+T +
Sbjct: 181 HESSDLRKKYSPQQYLDLMASTLHSQLKRLHGYGARKYVVGGIGLVGCAPSQRKRSETED 240

Query: 241 CDEEVNNWAAEYNVALKSMLQRLKMELKGIAFSYFDVYAVMSNFVRTPSSYGDSQRLEVF 300
           CDEEVNNWAA YN ALKS L+ LKMEL  I+FSYFDVY VMSNF+ +PSSYG ++ ++  
Sbjct: 241 CDEEVNNWAAIYNTALKSKLETLKMELNDISFSYFDVYQVMSNFIHSPSSYGFTE-IKSA 300

Query: 301 VVGLG 305
             GLG
Sbjct: 301 CCGLG 304

[Close]

BLAST of Spg000074 vs. TAIR 10
Match: AT5G55050.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 288.5 bits (737), Expect = 6.3e-78
Identity = 151/296 (51.01%), Postives = 202/296 (68.24%), Query Frame = 0


Query: 6   LSLSLVFLFSLLFSVSDG--------GLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPHN 65
           L++ L+FL  L F    G          +P +YVFGDSLVD GNNNYL +S++KAN+PHN
Sbjct: 9   LTIFLLFLGLLRFDSFPGLEAATGKLASIPGLYVFGDSLVDAGNNNYLPISISKANYPHN 68

Query: 66  GLDFPTKNPTGRFSNGKNAADYVAEKVGLPTSPPYLSLISKFRKIINTTPFTTGVSFASG 125
           G+DFP K PTGRF NGKNAAD +AEK GLP  PPYLSL    ++    +   TGV+FASG
Sbjct: 69  GVDFPNKKPTGRFCNGKNAADAIAEKFGLPLPPPYLSLRGLLKREKRKSAAVTGVNFASG 128

Query: 126 GAGIFNETNKLFKQSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSKSLFTVVIGSNDIF 185
           GAGIFN +++   Q++ + +Q+  + S+++ V+ +L  SAAQ+HLSKSLFTVVIGSND+F
Sbjct: 129 GAGIFNSSDQKLGQAIPLSKQVNNWLSIHEEVM-KLEPSAAQLHLSKSLFTVVIGSNDLF 188

Query: 186 GYHESSDLRNKYSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQR-KRSK 245
            Y  S  LR + +PQQY  LMA  L+ QLKR++  GAR++++ GV  +GCTP +R K S 
Sbjct: 189 DYFGSFKLRRQSNPQQYTQLMADKLKEQLKRIHDSGARRFLIIGVAQIGCTPGKRAKNST 248

Query: 246 TGECDEEVNNWAAEYNVALKSMLQRLKMELKG-IAFSYFDVYAVMSNFVRTPSSYG 292
             ECDE  N W + YN AL  MLQ+LK EL+G I ++YFD Y  + + +  P+ YG
Sbjct: 249 LHECDEGANMWCSLYNEALVKMLQQLKQELQGSITYTYFDNYKSLHDIISNPARYG 303

[Close]

BLAST of Spg000074 vs. TAIR 10
Match: AT5G37690.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 206.1 bits (523), Expect = 4.1e-53
Identity = 103/264 (39.02%), Postives = 157/264 (59.47%), Query Frame = 0


Query: 30  YVFGDSLVDVGNNNYLKLSLAKANFPHNGLDFPTKNPTGRFSNGKNAADYVAEKVGLPTS 89
           Y+FGDSL +VGNNN+L+ SLA+A+FP+ G+DF     TGRF+NG+   D ++ K+G+ + 
Sbjct: 29  YIFGDSLTEVGNNNFLQYSLARADFPYYGVDFSGGKATGRFTNGRTIGDIISTKLGILSP 88

Query: 90  PPYLSLISKFRKIINTTPFTTGVSFASGGAGIFNETNKLFKQSVAMDQQIEYYSSVYQYV 149
           PPYLSL        N   F +G+++ASGGAGI NET   F Q +  + QI  +    + +
Sbjct: 89  PPYLSLSQ------NDDAFLSGINYASGGAGILNETGIYFIQRLTFNDQINCFKKTKEVI 148

Query: 150 VGELGSSAAQVHLSKSLFTVVIGSNDIFGYHESSDLRN--KYSPQQYVDLMASTLESQLK 209
             ++G  AA  H++ +++ + +GSND         + +  +Y+  ++V+L+ STL +QL 
Sbjct: 149 RAKIGDGAANKHVNDAMYFIGLGSNDYVNNFLQPFMADGQQYTHDEFVELLTSTLHNQLT 208

Query: 210 RLYGYGARKYVVAGVGLVGCTPSQRKRSKTGECDEEVNNWAAEYNVALKSMLQRLKMELK 269
            +Y  GARK +  G+G +GC PSQR +SKT  C   VN W  E+N   K +L  L   L 
Sbjct: 209 TIYKLGARKVIFHGLGPLGCIPSQRVKSKTRMCLNRVNEWVLEFNSRTKKLLIDLNKRLP 268

Query: 270 GIAFSYFDVYAVMSNFVRTPSSYG 292
           G  FS+ D Y  + + +  P+ YG
Sbjct: 269 GAKFSFADTYPAVLDLINNPTHYG 286

[Close]

BLAST of Spg000074 vs. TAIR 10
Match: AT3G50400.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 183.0 bits (463), Expect = 3.7e-46
Identity = 116/289 (40.14%), Postives = 169/289 (58.48%), Query Frame = 0


Query: 14  FSLLFSVSDGGLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPHNGLDF-PTK-NPTGRFS 73
           FS + S  D   + A +VFGDSLVD GNNNYL+ +L++AN P NG+DF P++ NPTGRF+
Sbjct: 20  FSRVASAGDQRALAASFVFGDSLVDAGNNNYLQ-TLSRANSPPNGIDFKPSRGNPTGRFT 79

Query: 74  NGKNAADYVAEKVGLPT-SPPYLSLISKFRKIINTTPFTTGVSFASGGAGIFNETNKLFK 133
           NG+  AD V EK+G  + + PYL+  +    ++N      GV++ASGG GI N T  +F 
Sbjct: 80  NGRTIADIVGEKLGQQSYAVPYLAPNASGEALLN------GVNYASGGGGILNATGSVFV 139

Query: 134 QSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSK-SLFTVVIGSNDIFGYH----ESSDL 193
             + MD Q++Y+++  +     LG   A+ ++ K SLF+VVIGSND    +     ++  
Sbjct: 140 NRLGMDIQVDYFTNTRKQFDKLLGQDKARDYIRKRSLFSVVIGSNDFLNNYLVPFVAAQA 199

Query: 194 RNKYSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQRKRSKTG--ECDEE 253
           R   +P+ +VD M S L +QLKRLY   ARK+VV  V  +GC P Q+  ++    +C + 
Sbjct: 200 RLTQTPETFVDDMISHLRNQLKRLYDMDARKFVVGNVAPIGCIPYQKSINQLNDKQCVDL 259

Query: 254 VNNWAAEYNVALKSMLQ-RLKMELKGIAFSYFDVYAVMSNFVRTPSSYG 292
            N  A +YN  LK +L   LK  LK   F Y +VY +  + +     YG
Sbjct: 260 ANKLAIQYNARLKDLLTVELKDSLKDAHFVYANVYDLFMDLIVNFKDYG 301

[Close]

BLAST of Spg000074 vs. TAIR 10
Match: AT1G74460.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 179.1 bits (453), Expect = 5.4e-45
Identity = 99/283 (34.98%), Postives = 152/283 (53.71%), Query Frame = 0


Query: 11  VFLFSLLFSVSDGGLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPHNGLDFPTKNPTGRF 70
           +F+  ++ +++        ++FGDSL DVGNN  L  SLA AN P  G+DF    P GRF
Sbjct: 6   IFVLFIVLAINGYDCKIVQFIFGDSLSDVGNNKNLPRSLATANLPFYGIDFGNGLPNGRF 65

Query: 71  SNGKNAADYVAEKVGLPTSPPYLSLISKFRKIINTTPFTTGVSFASGGAGIFNETNKLFK 130
           +NG+  +D + +K+GLP    +L        I+       GV++ASGG GI NET   F 
Sbjct: 66  TNGRTVSDIIGDKIGLPRPVAFLDPSMNEDVILE-----NGVNYASGGGGILNETGGYFI 125

Query: 131 QSVAMDQQIEYYSSVYQYVVGELGSSAAQVHLSKSLFTVVIGSNDIFGYHESSDLRN--K 190
           Q  ++ +QIE +      VV ++G   A      + + V +GSND    +      +  K
Sbjct: 126 QRFSLWKQIELFQGTQDVVVAKIGKKEADKFFQDARYVVALGSNDFINNYLMPVYSDSWK 185

Query: 191 YSPQQYVDLMASTLESQLKRLYGYGARKYVVAGVGLVGCTPSQRKRSKTGECDEEVNNWA 250
           Y+ Q +VD +  TLESQLK L+  GARK +V G+G +GC P QR  S  G C  + +N A
Sbjct: 186 YNDQTFVDYLMETLESQLKVLHSLGARKLMVFGLGPMGCIPLQRALSLDGNCQNKASNLA 245

Query: 251 AEYNVALKSMLQRLKMELKGIAFSYFDVYAVMSNFVRTPSSYG 292
             +N A  +ML  L+ +L   ++ + + Y ++++ +  P  YG
Sbjct: 246 KRFNKAATTMLLDLETKLPNASYRFGEAYDLVNDVITNPKKYG 283

[Close]

BLAST of Spg000074 vs. TAIR 10
Match: AT1G71691.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 172.2 bits (435), Expect = 6.6e-43
Identity = 101/271 (37.27%), Postives = 144/271 (53.14%), Query Frame = 0


Query: 24  GLVPAMYVFGDSLVDVGNNNYLKLSLAKANFPHNGLDFPTKNPTGRFSNGKNAADYVAEK 83
           G+VPA++VFGDSL+D GNNN +  S AKAN+   G+DF    PTGRF NG    D +A+ 
Sbjct: 51  GIVPALFVFGDSLIDNGNNNNIP-SFAKANYFPYGIDF-NGGPTGRFCNGLTMVDGIAQL 110

Query: 84  VGLPTSPPYLSLISKFRKIINTTPFTTGVSFASGGAGIFNETNKLFKQSVAMDQQIEYYS 143
           +GLP  P Y                  GV++AS  AGI  +T   F   +  DQQI  + 
Sbjct: 111 LGLPLIPAYSEATG--------DQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFE 170

Query: 144 SVYQYVVGELGSSAAQV-HLSKSLFTVVIGSNDIFGYH--ESSDLRNKYSPQQYVDLMAS 203
           +    V  + G + A    +++SLF + +GSND    +   +   RN+Y+ QQ+ DL+  
Sbjct: 171 TTLDQVASKSGGAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQYNSQQFGDLLVQ 230

Query: 204 TLESQLKRLYGYGARKYVVAGVGLVGCTPSQRKRSKTGECDEEVNNWAAEYNVALKSMLQ 263
               QL RLY  G RK+VVAG+G +GC PS   +   G+C EEVN     +N  +K+M+ 
Sbjct: 231 HYTDQLTRLYNLGGRKFVVAGLGRMGCIPSILAQGNDGKCSEEVNQLVLPFNTNVKTMIS 290

Query: 264 RLKMELKGIAFSYFDVYAVMSNFVRTPSSYG 292
            L   L    F Y D+  +  + V   ++YG
Sbjct: 291 NLNQNLPDAKFIYLDIAHMFEDIVANQAAYG 311

The following BLAST results are available for this feature:

BLAST of Spg000074 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022932219.1	2.9e-133	82.88	GDSL esterase/lipase At5g55050 [Cucurbita moschata]	[more]
KAG7015527.1	4.9e-133	83.33	GDSL esterase/lipase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_038903279.1	8.4e-133	82.14	GDSL esterase/lipase At5g55050 [Benincasa hispida]	[more]
XP_023552078.1	3.2e-132	83.33	GDSL esterase/lipase At5g55050 [Cucurbita pepo subsp. pepo]	[more]
XP_008449153.1	3.2e-132	80.19	PREDICTED: GDSL esterase/lipase At5g55050 [Cucumis melo]	[more]

BLAST of Spg000074 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FIA1	8.8e-77	51.01	GDSL esterase/lipase At5g55050 OS=Arabidopsis thaliana OX=3702 GN=At5g55050 PE=2...	[more]
Q9FHQ1	5.8e-52	39.02	GDSL esterase/lipase At5g37690 OS=Arabidopsis thaliana OX=3702 GN=At5g37690 PE=2...	[more]
O23470	1.2e-46	38.31	GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3...	[more]
Q9M2R9	5.2e-45	40.14	GDSL esterase/lipase At3g50400 OS=Arabidopsis thaliana OX=3702 GN=At3g50400 PE=2...	[more]
Q9CA68	7.6e-44	34.98	GDSL esterase/lipase At1g74460 OS=Arabidopsis thaliana OX=3702 GN=At1g74460 PE=2...	[more]

BLAST of Spg000074 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EWE7	1.4e-133	82.88	GDSL esterase/lipase At5g55050 OS=Cucurbita moschata OX=3662 GN=LOC111438588 PE=...	[more]
A0A1S3BM17	1.5e-132	80.19	GDSL esterase/lipase At5g55050 OS=Cucumis melo OX=3656 GN=LOC103491100 PE=3 SV=1	[more]
A0A6J1DE65	2.6e-132	82.94	GDSL esterase/lipase At5g55050-like OS=Momordica charantia OX=3673 GN=LOC1110192...	[more]
A0A6J1HMQ2	4.5e-132	82.19	GDSL esterase/lipase At5g55050 OS=Cucurbita maxima OX=3661 GN=LOC111465054 PE=3 ...	[more]
A0A0A0L695	3.6e-129	78.69	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G075990 PE=3 SV=1	[more]

BLAST of Spg000074 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55050.1	6.3e-78	51.01	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT5G37690.1	4.1e-53	39.02	SGNH hydrolase-type esterase superfamily protein	[more]
AT3G50400.1	3.7e-46	40.14	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G74460.1	5.4e-45	34.98	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G71691.2	6.6e-43	37.27	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 30..283 e-value: 3.6E-24 score: 86.5
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 20..298 e-value: 6.8E-64 score: 218.3
None	No IPR available	PANTHER	PTHR45648	GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)	coord: 6..292
None	No IPR available	PANTHER	PTHR45648:SF90	GDSL-LIKE LIPASE/ACYLHYDROLASE SUPERFAMILY PROTEIN-RELATED	coord: 6..292
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 29..256
IPR035669	GDSL lipase/esterase-like, plant	CDD	cd01837	SGNH_plant_lipase_like	coord: 27..291 e-value: 1.01773E-102 score: 301.457

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg000074.1	Spg000074.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds