Sgr030002 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr030002
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	gibberellin 20-oxidase-like protein
Location	tig00153554: 2012608 .. 2014554 (+)
RNA-Seq Expression	Sgr030002
Synteny	Sgr030002

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCGAATCTGAAACGACGAGCTGCGTGGTGGAGCTTCCAGTGATCGATCTTTCTCAGCCAAATTTGAACGACGAATCGAGTCTCTCTGCAGCCTGCATGGAGTGGGGCTTCTTCTACATCACCAACCATGGTGTTTCCATGGAGTTTTACCGGAAACTCATCTCCCTCGCCGATCAACTCTTCGCCCTTCCTCAAGACTCCAAAACTAACGTCGGAATTTCGTCGTACGTCCCGAGGTTCGTCGCGTCGCCTTTCTTTGAGAGCTTCAAGGTTTCTGGGCCCGACTTCTCTGCCTCTGCTCGAGCCTTGACGGAAGCGCTCTTCGCCGACGGCCACCGCAATACTGAATTCAGGTAAGAATTGATTAAGAAGCATACATGTATTAATACACAAAAATATGAAAATCAAAGTATTTTAATTTTATAAGGTTTTTGTGTTATCTGATTTCCTATTAAAAGAAAATTGGTCAAATTACAAATTTAGTTCGCGCATAGTTTTCAAAATTTTAGTTTATTCCTTATATTTTAAAAGTTTCAATTTGGTATCTTTGGTCAAATCTCGTAAATTATTTATAATTTGTCTTTTACGCATTAGAAAAAAAAAATGTTAATATGGTTAAGGGACGATCCGTGAGGTTTGACTAATGACCAAATTGAAAGTTTTAAAATTATAAAGACCAAAATGTAATATAATTAAGTCAAAACCATATGGACTAAATTGAAAATTTTAAAATTATAAAGATTAAATTAAAATTATGCTCAAATCATAAAGATCAAATTTGTAATTTAACAAAATAGAAATAATTTTGTTTAATAATGATTTTTAATTGACTATTCAATAATTATCAAATTTTATTTTATCTTTTAGCTATAAAAATGAAATATTTTAATTTTATAATTTCAATATTTATAAAATATTAGAATTAGAAAATAAAATTGAGATAAAAGAAATAAAAGTTAATAAAGTTAAATGCATCTAGATATATTAGAGAAAATTGAACTGTCAATGCTTTAGTAATTAACGCATTTTAGACCTTTTTCAAAGTCAAAGTTTCAATCTCCTAACAGCAGCGTTAACTAAAAAAAATAAAAAATGAGAGAGAGAAGCGAGAATGAAGTAACATGAGTAAAAGTGAGTTGAATGACTTTTCTGATATACATTTTTAAGTGGCCGCGGAATCACTCATTAATTGTTTCTTTCAAATGCGGTGTTTTAAGATAATCAAACACTAATAAATTTTCACAATTGTGCAAATTATTAAACAGAATGCTCATTGTTCGTTATTGTAATTTTGTTTCTGAAACTAACCAATCTCTCTCTTGTGAGTTGAATTTGGTTTGCAGCAATCTACTCGAGGAATATGGAAGCAAAATGACAGACCTATCAAAAAGAATAGTAAAGCTATTGTTAAAAATCTTGGGAGAGAATTTTGAAAACAAATTCTATGATTCTGAGTTCAGCAACTGCAATGGCTACCTGAGGATAAACAACTACTCTCCTGGAGACTCCAAGGAAGAAGAAGAAGTGGAAGGGCTGGGAAAGCATACCGACATTAGCTGCGTGACGATTCTGTTCCAAGACGACGTCGGCGGGCTTCAGATGAGATCGAAAGGAGGAGATTGGACGGACATAAGGCCATGCGAGGACGCGGTAGTGGTGAACATTGGGGATTTTCTGCAGGCATGGAGCAACGAGAGGCTGAGATCGGCGGAGCACCGAGTGGTTCTGAGGCGAAATGTGAAGAGATTCTCTGTGGCGTTCTTCTGGTCGTACGGAGAGGAGAGAGAGATCTATGCGCCGAGTGAGGTCGTCGGAGAAGGAAATGCGAGGCTGTATCGGCCGTTCTCGACTAAAGATTACCGGATGTTTAGGGCAACTGGTGTGAGAGACAACTATGAGAAAGTCGGAGCCACTGTGAAGGAATTTGCTGGAATTGTAGGATGA

mRNA sequence

ATGTCCGAATCTGAAACGACGAGCTGCGTGGTGGAGCTTCCAGTGATCGATCTTTCTCAGCCAAATTTGAACGACGAATCGAGTCTCTCTGCAGCCTGCATGGAGTGGGGCTTCTTCTACATCACCAACCATGGTGTTTCCATGGAGTTTTACCGGAAACTCATCTCCCTCGCCGATCAACTCTTCGCCCTTCCTCAAGACTCCAAAACTAACGTCGGAATTTCGTCGTACGTCCCGAGGTTCGTCGCGTCGCCTTTCTTTGAGAGCTTCAAGGTTTCTGGGCCCGACTTCTCTGCCTCTGCTCGAGCCTTGACGGAAGCGCTCTTCGCCGACGGCCACCGCAATACTGAATTCAGCAATCTACTCGAGGAATATGGAAGCAAAATGACAGACCTATCAAAAAGAATAGTAAAGCTATTGTTAAAAATCTTGGGAGAGAATTTTGAAAACAAATTCTATGATTCTGAGTTCAGCAACTGCAATGGCTACCTGAGGATAAACAACTACTCTCCTGGAGACTCCAAGGAAGAAGAAGAAGTGGAAGGGCTGGGAAAGCATACCGACATTAGCTGCGTGACGATTCTGTTCCAAGACGACGTCGGCGGGCTTCAGATGAGATCGAAAGGAGGAGATTGGACGGACATAAGGCCATGCGAGGACGCGGTAGTGGTGAACATTGGGGATTTTCTGCAGGCATGGAGCAACGAGAGGCTGAGATCGGCGGAGCACCGAGTGGTTCTGAGGCGAAATGTGAAGAGATTCTCTGTGGCGTTCTTCTGGTCGTACGGAGAGGAGAGAGAGATCTATGCGCCGAGTGAGGTCGTCGGAGAAGGAAATGCGAGGCTGTATCGGCCGTTCTCGACTAAAGATTACCGGATGTTTAGGGCAACTGGTGTGAGAGACAACTATGAGAAAGTCGGAGCCACTGTGAAGGAATTTGCTGGAATTGTAGGATGA

Coding sequence (CDS)

ATGTCCGAATCTGAAACGACGAGCTGCGTGGTGGAGCTTCCAGTGATCGATCTTTCTCAGCCAAATTTGAACGACGAATCGAGTCTCTCTGCAGCCTGCATGGAGTGGGGCTTCTTCTACATCACCAACCATGGTGTTTCCATGGAGTTTTACCGGAAACTCATCTCCCTCGCCGATCAACTCTTCGCCCTTCCTCAAGACTCCAAAACTAACGTCGGAATTTCGTCGTACGTCCCGAGGTTCGTCGCGTCGCCTTTCTTTGAGAGCTTCAAGGTTTCTGGGCCCGACTTCTCTGCCTCTGCTCGAGCCTTGACGGAAGCGCTCTTCGCCGACGGCCACCGCAATACTGAATTCAGCAATCTACTCGAGGAATATGGAAGCAAAATGACAGACCTATCAAAAAGAATAGTAAAGCTATTGTTAAAAATCTTGGGAGAGAATTTTGAAAACAAATTCTATGATTCTGAGTTCAGCAACTGCAATGGCTACCTGAGGATAAACAACTACTCTCCTGGAGACTCCAAGGAAGAAGAAGAAGTGGAAGGGCTGGGAAAGCATACCGACATTAGCTGCGTGACGATTCTGTTCCAAGACGACGTCGGCGGGCTTCAGATGAGATCGAAAGGAGGAGATTGGACGGACATAAGGCCATGCGAGGACGCGGTAGTGGTGAACATTGGGGATTTTCTGCAGGCATGGAGCAACGAGAGGCTGAGATCGGCGGAGCACCGAGTGGTTCTGAGGCGAAATGTGAAGAGATTCTCTGTGGCGTTCTTCTGGTCGTACGGAGAGGAGAGAGAGATCTATGCGCCGAGTGAGGTCGTCGGAGAAGGAAATGCGAGGCTGTATCGGCCGTTCTCGACTAAAGATTACCGGATGTTTAGGGCAACTGGTGTGAGAGACAACTATGAGAAAGTCGGAGCCACTGTGAAGGAATTTGCTGGAATTGTAGGATGA

Protein sequence

MSESETTSCVVELPVIDLSQPNLNDESSLSAACMEWGFFYITNHGVSMEFYRKLISLADQLFALPQDSKTNVGISSYVPRFVASPFFESFKVSGPDFSASARALTEALFADGHRNTEFSNLLEEYGSKMTDLSKRIVKLLLKILGENFENKFYDSEFSNCNGYLRINNYSPGDSKEEEEVEGLGKHTDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVVVNIGDFLQAWSNERLRSAEHRVVLRRNVKRFSVAFFWSYGEEREIYAPSEVVGEGNARLYRPFSTKDYRMFRATGVRDNYEKVGATVKEFAGIVG

Homology

[Close]

BLAST of Sgr030002 vs. NCBI nr
Match: XP_022141254.1 (gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia])

HSP 1 Score: 514.6 bits (1324), Expect = 6.0e-142
Identity = 259/320 (80.94%), Postives = 283/320 (88.44%), Query Frame = 0


Query: 1   MSESETTSCVVELPVIDLSQPNLNDE--SSLSAACMEWGFFYITNHGVSMEFYRKLISLA 60
           MSES+T    VELPVIDLSQP L++    SL AAC EWGFFYITNHGVS EFY+KLIS+A
Sbjct: 1   MSESQT---CVELPVIDLSQP-LSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIA 60

Query: 61  DQLFALPQDSKTNVGISSYVPRFVASPFFESFKVSGPDFSASARALTEALFADGHRNTEF 120
           D+LF LPQD+K  VGISSYVPRF ASPFFESFK+SGPDFSASA+ LTEAL   GH NT+F
Sbjct: 61  DELFRLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALL--GHHNTQF 120

Query: 121 SNLLEEYGSKMTDLSKRIVKLLLKILGENFENKFYDSEFSNCNGYLRINNYSPGDSKEEE 180
           SNLLEEYGSKMT+LSKRIVKLLLK+LG+NFENKFYD EF NC+GYLRINNYSP DS   E
Sbjct: 121 SNLLEEYGSKMTBLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDS--NE 180

Query: 181 EVEGLGKHTDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVVVNIGDFLQAWSNERL 240
           EVEGLGKHTDISCVTILFQD +GGLQMRSKGG+W DIRPCE+A+VVNIGDF+QAWSNERL
Sbjct: 181 EVEGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERL 240

Query: 241 RSAEHRVVLRRNVKRFSVAFFWSYGEEREIYAPSEVVGEGNARLYRPFSTKDYRMFRATG 300
           RSAEHRVVLR+NVKRFS+AFFWSY ++ EIYAPSEVVGEGN RLYR FSTK+YRMFRATG
Sbjct: 241 RSAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATG 300

Query: 301 VRDNYEKVGATVKEFAGIVG 319
            R  YEKVGA VKEFAGIVG
Sbjct: 301 ARGTYEKVGANVKEFAGIVG 312

[Close]

BLAST of Sgr030002 vs. NCBI nr
Match: XP_022141253.1 (gibberellin 20-oxidase-like protein [Momordica charantia])

HSP 1 Score: 493.4 bits (1269), Expect = 1.4e-135
Identity = 248/323 (76.78%), Postives = 281/323 (87.00%), Query Frame = 0


Query: 1   MSESETTSCVVELPVIDLSQPNLNDE--SSLSAACMEWGFFYITNHGVSMEFYRKLISLA 60
           MSES+T    VELPVID+ + +L++   SSL+ AC EWGFFYI NHGVS EFY +LIS+A
Sbjct: 1   MSESQT---CVELPVIDVYE-DLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIA 60

Query: 61  DQLFALPQDSKTNVGISSYVPRFVASPFFESFKVSGPDFSASARALTEALFADGHRNTEF 120
           D+LF LPQ++K  VGIS YVPRF+ASPFFESFK +GPDFSASA +LTEALF   H NTEF
Sbjct: 61  DELFRLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALF--DHPNTEF 120

Query: 121 SNLLEEYGSKMTDLSKRIVKLLLKILGENFENKFYDSEFSNCNGYLRINNYSPGDSKEE- 180
           SNLLEEYGSKM +LSKRIV+LLLKIL + FENKFYDSEFSNC G L INNYSP DS +E 
Sbjct: 121 SNLLEEYGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKED 180

Query: 181 --EEVEGLGKHTDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVVVNIGDFLQAWSN 240
             EE+EGLGKHTDISCVTILFQD+VGGLQMRSKGG+W DI+PCEDA+VVNIGDF+QAWSN
Sbjct: 181 KVEEIEGLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSN 240

Query: 241 ERLRSAEHRVVLRRNVKRFSVAFFWSYGEEREIYAPSEVVGEGNARLYRPFSTKDYRMFR 300
           ERLRSAEHRVVLR+NVKRFS++FFWSY ++REIYAPSEVVGEGN RLYRPFS K+Y+MFR
Sbjct: 241 ERLRSAEHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFR 300

Query: 301 ATGVRDNYEKVGATVKEFAGIVG 319
           AT  R NYEKVGATVK+FAGIVG
Sbjct: 301 ATSARGNYEKVGATVKDFAGIVG 317

[Close]

BLAST of Sgr030002 vs. NCBI nr
Match: XP_022140867.1 (gibberellin 20-oxidase-like protein [Momordica charantia])

HSP 1 Score: 477.6 bits (1228), Expect = 8.2e-131
Identity = 239/323 (73.99%), Postives = 275/323 (85.14%), Query Frame = 0


Query: 4   SETTSCVVELPVIDLSQPNLNDE--SSLSAACMEWGFFYITNHGVSMEFYRKLISLADQL 63
           SE+ S  VELPVID+ + +L++   SSLS AC EWGFFYI NHGVS EFY+KLIS+ADQL
Sbjct: 2   SESKSYTVELPVIDVYE-DLSESALSSLSEACSEWGFFYIRNHGVSKEFYQKLISIADQL 61

Query: 64  FALPQDSKTNVGISSYVPRFVASPFFESFKVSGPDFSASARALTEALFADGHRNTEFSNL 123
           F LP ++K  VG S Y+PRF+ASPF+ESFK SGPDFSASA  LTE L   G  + EFSNL
Sbjct: 62  FCLPHETKNKVGFSCYIPRFIASPFYESFKFSGPDFSASAHHLTETLL--GKHDAEFSNL 121

Query: 124 LEEYGSKMTDLSKRIVKLLLKILGENFENKFYDSEFSNCNGYLRINNYSPGDSKEE---E 183
           L+EYGSKMTD+S+RIVKLLLKILG+NFENKFYDSEFSNC+GYLR+NNYSP DS E    E
Sbjct: 122 LKEYGSKMTDISRRIVKLLLKILGDNFENKFYDSEFSNCDGYLRMNNYSPQDSNENVKIE 181

Query: 184 EVEGLGKHTDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVVVNIGDFLQAWSNERL 243
           EVEGLGKHTDISCVTILFQD VGGLQMRSK G+W DI+PCEDA+VVNIGDF+ AWSNE+L
Sbjct: 182 EVEGLGKHTDISCVTILFQDHVGGLQMRSKEGEWMDIKPCEDALVVNIGDFMHAWSNEKL 241

Query: 244 RSAEHRVVLRRN-VKRFSVAFFWSYGEEREIYAPSEVVGEGNARLYRPFSTKDYRMFRAT 303
           RSAEHRVVLR+N VKRFS+ F WS+ +++EIYAPSEVVGEGNARLY+PF TK+YR+FRAT
Sbjct: 242 RSAEHRVVLRQNSVKRFSMVFIWSFEDDKEIYAPSEVVGEGNARLYKPFLTKEYRIFRAT 301

Query: 304 --GVRDNYEKVGATVKEFAGIVG 319
             G R+NYEKVGA V +FAGIVG
Sbjct: 302 PRGARENYEKVGANVTDFAGIVG 321

[Close]

BLAST of Sgr030002 vs. NCBI nr
Match: XP_022141255.1 (gibberellin 20-oxidase-like protein isoform X2 [Momordica charantia])

HSP 1 Score: 410.2 bits (1053), Expect = 1.6e-110
Identity = 219/320 (68.44%), Postives = 239/320 (74.69%), Query Frame = 0


Query: 1   MSESETTSCVVELPVIDLSQPNLNDE--SSLSAACMEWGFFYITNHGVSMEFYRKLISLA 60
           MSES+T    VELPVIDLSQP L++    SL AAC EWGFFYITNHGVS EFY+KLIS+A
Sbjct: 1   MSESQT---CVELPVIDLSQP-LSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIA 60

Query: 61  DQLFALPQDSKTNVGISSYVPRFVASPFFESFKVSGPDFSASARALTEALFADGHRNTEF 120
           D+LF LPQD+K  VGISSYVPRF ASPFFESFK+SGPDFSASA+ LTEAL   GH NT+F
Sbjct: 61  DELFRLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALL--GHHNTQF 120

Query: 121 SNLLEEYGSKMTDLSKRIVKLLLKILGENFENKFYDSEFSNCNGYLRINNYSPGDSKEEE 180
                                                         RINNYSP DS   E
Sbjct: 121 ----------------------------------------------RINNYSPRDS--NE 180

Query: 181 EVEGLGKHTDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVVVNIGDFLQAWSNERL 240
           EVEGLGKHTDISCVTILFQD +GGLQMRSKGG+W DIRPCE+A+VVNIGDF+QAWSNERL
Sbjct: 181 EVEGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERL 240

Query: 241 RSAEHRVVLRRNVKRFSVAFFWSYGEEREIYAPSEVVGEGNARLYRPFSTKDYRMFRATG 300
           RSAEHRVVLR+NVKRFS+AFFWSY ++ EIYAPSEVVGEGN RLYR FSTK+YRMFRATG
Sbjct: 241 RSAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATG 266

Query: 301 VRDNYEKVGATVKEFAGIVG 319
            R  YEKVGA VKEFAGIVG
Sbjct: 301 ARGTYEKVGANVKEFAGIVG 266

[Close]

BLAST of Sgr030002 vs. NCBI nr
Match: XP_031265650.1 (gibberellin 20-oxidase-like protein [Pistacia vera])

HSP 1 Score: 395.6 bits (1015), Expect = 4.1e-106
Identity = 194/321 (60.44%), Postives = 249/321 (77.57%), Query Frame = 0


Query: 1   MSESETTSCVVELPVIDLSQP-NLNDESSLSAACMEWGFFYITNHGVSMEFYRKLISLAD 60
           M+ES+TT   VELPV+D+SQP + +  S+LSAAC EWGFFYITNHGVS + YRKL SL++
Sbjct: 1   MAESQTT---VELPVLDISQPLSTSKLSALSAACKEWGFFYITNHGVSKDLYRKLHSLSN 60

Query: 61  QLFALPQDSKTNVG----ISSYVPRFVASPFFESFKVSGPDFSASARALTEALFADGHRN 120
            LF LP ++K  VG    I +Y P F+ASPFFESF+VSGPDFS+SA++  EAL  +   N
Sbjct: 61  HLFNLPYETKVKVGPFSSIKTYTPHFIASPFFESFRVSGPDFSSSAQSSVEALLKE--PN 120

Query: 121 TEFSNLLEEYGSKMTDLSKRIVKLLLKILGENFENKFYDSEFSNCNGYLRINNYSPGDSK 180
           ++FS  L+EYG KMT+LSK IV+ +L  LG++F+ KFY+SEFS C+GYLRI NYSP ++ 
Sbjct: 121 SDFSETLKEYGRKMTELSKAIVQFVLMSLGKDFQKKFYESEFSKCHGYLRIINYSPPENV 180

Query: 181 EEEEVEGLGKHTDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVVVNIGDFLQAWSN 240
            E++VEGLG HTD+SCVTI++QD++GGLQ+RSK G W DI PCED +VVNIGD + AWSN
Sbjct: 181 VEKQVEGLGMHTDMSCVTIVYQDEIGGLQVRSKEGTWMDINPCEDTLVVNIGDLMHAWSN 240

Query: 241 ERLRSAEHRVVLRRNVKRFSVAFFWSYGEEREIYAPSEVVGEGNARLYRPFSTKDYRMFR 300
            RLRS+EHRVVL+RN  RFS+AFFW + +E+ I AP EVVGE + R+Y+ F  KDY  FR
Sbjct: 241 GRLRSSEHRVVLKRNANRFSLAFFWCFEDEKNISAPDEVVGEEDNRVYKTFICKDYLRFR 300

Query: 301 ATGVRDNYEKVGATVKEFAGI 317
            +  +  +EKVG TVK+FAGI
Sbjct: 301 ESSEKGKFEKVGFTVKQFAGI 316

[Close]

BLAST of Sgr030002 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.4e-85
Identity = 161/322 (50.00%), Postives = 227/322 (70.50%), Query Frame = 0


Query: 11  VELPVIDLSQPNLNDES--SLSAACMEWGFFYITNHGVSMEFYRKLISLADQLFALPQDS 70
           +ELPV D+S+P L++ S  SL  AC EWGFFY+TNHGVS + Y+KL   +  +F L  + 
Sbjct: 3   LELPVFDISKP-LSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEE 62

Query: 71  KTNVGISSYVPRFVASPFFESFKVSGPDFSASARALTEALFADGHRNTEFSNLLEEYGSK 130
           K  +G S+Y PRF+ASPFFES +VSGPDF ASA++  +A F+D   + EFS L++EYG K
Sbjct: 63  KMKMGASNYTPRFIASPFFESLRVSGPDFYASAKSSVDA-FSDQATDEEFSGLMKEYGEK 122

Query: 131 MTDLSKRIVKLLLKILGENFENKFYDSEFSNCNGYLRINNYS-PGDSK-------EEEEV 190
           MT L ++I+K +L   G++  +K+Y+SEF NC+GY RINNY+ P D +       E++ +
Sbjct: 123 MTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQDLI 182

Query: 191 EGLGKHTDISCVTILFQDDVGGLQMRSKGG-DWTDIRPCEDAVVVNIGDFLQAWSNERLR 250
           EGLG HTD+SC+TI+ QDD+GGLQ+R++ G    DI P ++A+VVN+GD L AW+N RLR
Sbjct: 183 EGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLLHAWTNGRLR 242

Query: 251 SAEHRVVLRRN---VKRFSVAFFWSYGEEREIYAPSEVVG--EGNARLYRPFSTKDYRMF 310
           S++HRV+L+R      RFS+AFFW + + + ++AP EVVG  EG  R++R F   DY  F
Sbjct: 243 SSQHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEG-MRVFRSFKCGDYLRF 302

Query: 311 RATGVRDNYEKVGATVKEFAGI 317
           R +  +  +EKVG TV++FA I
Sbjct: 303 RESNEKGKFEKVGDTVEDFARI 321

[Close]

BLAST of Sgr030002 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.9e-34
Identity = 101/324 (31.17%), Postives = 162/324 (50.00%), Query Frame = 0


Query: 2   SESETTSCVVE-----LPVIDLSQPNLNDE-------SSLSAACMEWGFFYITNHGVSME 61
           SES T +  ++     LPVIDLS     +E         + AA  EWGFF I NHG+  +
Sbjct: 24  SESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKD 83

Query: 62  FYRKLISLADQLFALPQDSKTNVGISSYVPRFVASPFFESFKVSGPDFSASAR-ALTEA- 121
            +  ++    +LF  P   K     S             S++   P  ++ A+ +++EA 
Sbjct: 84  VFEMMLLEEKKLFDQPFSVKVRERFSDLSK--------NSYRWGNPSATSPAQYSVSEAF 143

Query: 122 --LFADGHRNTEFSNLLEEYGSKMTDLSKRIVKLLLKILGE--NFENKFYDSEFSNCNGY 181
             + ++  R ++  N L            R+ +++ +ILG+  N  ++++++ F   N +
Sbjct: 144 HIILSEVSRISDDRNNLRTIVETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSF 203

Query: 182 LRINNYSPGDSKEEEEVEGLGKHTDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVV 241
           LR+N Y P  S    EV GL  HTD S +TIL QD +GGL++ +  G W  ++PC +A+ 
Sbjct: 204 LRLNKYHP--SVFGSEVFGLVPHTDTSFLTILSQDQIGGLELEN-NGQWISVKPCLEALT 263

Query: 242 VNIGDFLQAWSNERLRSAEHRVVLRRNVKRFSVAFFWSYGEEREIYAPSEVVGEGNARLY 301
           VNIGD  QA SN   +S  HRV+   N++R S+AFF         Y  +E+   G  + Y
Sbjct: 264 VNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVC------PYLETEIDCFGYPKKY 323

Query: 302 RPFSTKDYRMFRATGVRDNYEKVG 308
           R FS ++Y+      V++  +KVG
Sbjct: 324 RRFSFREYKEQSEHDVKETGDKVG 330

[Close]

BLAST of Sgr030002 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 138.3 bits (347), Expect = 1.5e-31
Identity = 102/315 (32.38%), Postives = 156/315 (49.52%), Query Frame = 0


Query: 11  VELPVIDLS-------QPNLNDESSLSAACMEWGFFYITNHGVSMEFYRKLISLADQLFA 70
           VELPVID+S       +     + +++ A  EWGFF + NHG+SM+   K+     ++F 
Sbjct: 40  VELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFR 99

Query: 71  LPQDSKTNVGISSYVPRFVASPFFESFKVSGPDFSASARAL--TEAL------FADGHRN 130
            P D K      S   +F A     S++   P  + S R L  +EA        +D    
Sbjct: 100 EPFDKK------SKSEKFSAG----SYRWGTPS-ATSIRQLSWSEAFHVPMTDISDNKDF 159

Query: 131 TEFSNLLEEYGSKMTDLSKRIVKLLLKILGENFENKFYDSEFSNCNGYLRINNYSPGDSK 190
           T  S+ +E++ S+   L+  + ++L +  G+N  + F+         YLR+N Y P    
Sbjct: 160 TTLSSTMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYPP--CP 219

Query: 191 EEEEVEGLGKHTDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVVVNIGDFLQAWSN 250
           +  EV GL  HTD   +TIL+QD VGGLQ+  K   W  ++P   A+++NIGD  QAWSN
Sbjct: 220 KPSEVYGLMPHTDSDFLTILYQDQVGGLQL-IKDNRWIAVKPNPKALIINIGDLFQAWSN 279

Query: 251 ERLRSAEHRVVLRRNVKRFSVAFFWSYGEEREIYAPSEVVGEGNARLYRPFSTKDYRMFR 310
              +S EHRV+    V+RFS A+F     +  I   S+         YR FS +++R   
Sbjct: 280 GMYKSVEHRVMTNPKVERFSTAYFMCPSYDAVIECSSD------RPAYRNFSFREFRQ-- 328

[Close]

BLAST of Sgr030002 vs. ExPASy Swiss-Prot
Match: Q9C899 (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 5.9e-31
Identity = 106/296 (35.81%), Postives = 146/296 (49.32%), Query Frame = 0


Query: 13  LPVIDLSQPNLNDESSLSAAC---MEWGFFYITNHGVSMEFYRKLISLADQLFALPQDSK 72
           +PVID+S  NL+++S   A C    EWGFF + NHGVSME    + +   + F LP + K
Sbjct: 62  IPVIDIS--NLDEKSVSKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFGLPVEEK 121

Query: 73  ----------TNVGI-SSYVPRFVASPFFESFKVSGPDFSASARALTEALFADGHRNTEF 132
                     TNV   +S+ P   A    E        F + A A    L+ D  R+   
Sbjct: 122 RKFSREKSLSTNVRFGTSFSPH--AEKALEWKDYLSLFFVSEAEA--SQLWPDSCRSETL 181

Query: 133 SNLLEEYGSKMTDLSKRIVKLLLKILGENFENKFYD-SEFSNCNGYLRIN-NYSPGDSKE 192
             + E         +K +VK LL+ LGEN   K  D ++ S   G  RIN NY P     
Sbjct: 182 EYMNE---------TKPLVKKLLRFLGENLNVKELDKTKESFFMGSTRINLNYYPICPNP 241

Query: 193 EEEVEGLGKHTDISCVTILFQDDVGGLQMRS-KGGDWTDIRPCEDAVVVNIGDFLQAWSN 252
           E  V G+G+H+D+S +TIL QD++GGL +RS   G W  + P   ++V+NIGD +Q  SN
Sbjct: 242 ELTV-GVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPISGSLVINIGDAMQIMSN 301

Query: 253 ERLRSAEHRVVLRRNVKRFSVAFFWSYGEEREIYAPSEVVGEGNARLYRPFSTKDY 292
            R +S EHRV+   +  R SV  F S   E  I    EV+  G   +Y+     DY
Sbjct: 302 GRYKSVEHRVLANGSYNRISVPIFVSPKPESVIGPLLEVIENGEKPVYKDILYTDY 341

[Close]

BLAST of Sgr030002 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 131.0 bits (328), Expect = 2.5e-29
Identity = 85/293 (29.01%), Postives = 141/293 (48.12%), Query Frame = 0


Query: 13  LPVIDLSQPNLNDESSLSAACMEWGFFYITNHGVSMEFYRKLISLADQLFALP-----QD 72
           +P+IDL  P+  ++  +  AC  WG F I+NHGV +   + +  L   LF LP     + 
Sbjct: 57  IPLIDLDHPDATNQ--IGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKS 116

Query: 73  SKTNVGISSYVPRFVASPFFESFKVSGPDFSASARALTEALFADGHRNTEFSNLLEEYGS 132
           +++  G+S Y    +AS F +     G   + S       L+   H N  + +++EEY  
Sbjct: 117 ARSETGVSGYGVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLN--YCDIVEEYEE 176

Query: 133 KMTDLSKRIVKLLLKILG---ENFENKFYDSEFSNCNGYLRINNYSPGDSKEEEEVEGLG 192
            M  L+ +++ L L  LG   E+ E     S+ +     L++N+Y      E +   GL 
Sbjct: 177 HMKKLASKLMWLALNSLGVSEEDIEWASLSSDLNWAQAALQLNHYPV--CPEPDRAMGLA 236

Query: 193 KHTDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVVVNIGDFLQAWSNERLRSAEHR 252
            HTD + +TIL+Q++  GLQ+      W  + P   ++VVN+GD     SN   +S  HR
Sbjct: 237 AHTDSTLLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHR 296

Query: 253 VVLRRNVKRFSVAFFWSYGEEREIYAPSEVVGEGNARLYRPFSTKDYRMFRAT 298
             + +   R SVAF W    + +I    ++V    + LY+  + K+Y   +AT
Sbjct: 297 ARVNQTRARLSVAFLWGPQSDIKISPVPKLVSPVESPLYQSVTWKEYLRTKAT 343

[Close]

BLAST of Sgr030002 vs. ExPASy TrEMBL
Match: A0A6J1CI26 (gibberellin 20-oxidase-like protein isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011697 PE=3 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.7e-142
Identity = 260/320 (81.25%), Postives = 283/320 (88.44%), Query Frame = 0


Query: 1   MSESETTSCVVELPVIDLSQPNLNDE--SSLSAACMEWGFFYITNHGVSMEFYRKLISLA 60
           MSES+T    VELPVIDLSQP L++    SL AAC EWGFFYITNHGVS EFY+KLIS+A
Sbjct: 1   MSESQT---CVELPVIDLSQP-LSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIA 60

Query: 61  DQLFALPQDSKTNVGISSYVPRFVASPFFESFKVSGPDFSASARALTEALFADGHRNTEF 120
           D+LF LPQD+K  VGISSYVPRF ASPFFESFK+SGPDFSASA+ LTEAL   GH NT+F
Sbjct: 61  DELFRLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALL--GHHNTQF 120

Query: 121 SNLLEEYGSKMTDLSKRIVKLLLKILGENFENKFYDSEFSNCNGYLRINNYSPGDSKEEE 180
           SNLLEEYGSKMTDLSKRIVKLLLK+LG+NFENKFYD EF NC+GYLRINNYSP DS   E
Sbjct: 121 SNLLEEYGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDS--NE 180

Query: 181 EVEGLGKHTDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVVVNIGDFLQAWSNERL 240
           EVEGLGKHTDISCVTILFQD +GGLQMRSKGG+W DIRPCE+A+VVNIGDF+QAWSNERL
Sbjct: 181 EVEGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERL 240

Query: 241 RSAEHRVVLRRNVKRFSVAFFWSYGEEREIYAPSEVVGEGNARLYRPFSTKDYRMFRATG 300
           RSAEHRVVLR+NVKRFS+AFFWSY ++ EIYAPSEVVGEGN RLYR FSTK+YRMFRATG
Sbjct: 241 RSAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATG 300

Query: 301 VRDNYEKVGATVKEFAGIVG 319
            R  YEKVGA VKEFAGIVG
Sbjct: 301 ARGTYEKVGANVKEFAGIVG 312

[Close]

BLAST of Sgr030002 vs. ExPASy TrEMBL
Match: A0A6J1CJY7 (gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC111011696 PE=3 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 7.0e-136
Identity = 248/323 (76.78%), Postives = 281/323 (87.00%), Query Frame = 0


Query: 1   MSESETTSCVVELPVIDLSQPNLNDE--SSLSAACMEWGFFYITNHGVSMEFYRKLISLA 60
           MSES+T    VELPVID+ + +L++   SSL+ AC EWGFFYI NHGVS EFY +LIS+A
Sbjct: 1   MSESQT---CVELPVIDVYE-DLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIA 60

Query: 61  DQLFALPQDSKTNVGISSYVPRFVASPFFESFKVSGPDFSASARALTEALFADGHRNTEF 120
           D+LF LPQ++K  VGIS YVPRF+ASPFFESFK +GPDFSASA +LTEALF   H NTEF
Sbjct: 61  DELFRLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALF--DHPNTEF 120

Query: 121 SNLLEEYGSKMTDLSKRIVKLLLKILGENFENKFYDSEFSNCNGYLRINNYSPGDSKEE- 180
           SNLLEEYGSKM +LSKRIV+LLLKIL + FENKFYDSEFSNC G L INNYSP DS +E 
Sbjct: 121 SNLLEEYGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKED 180

Query: 181 --EEVEGLGKHTDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVVVNIGDFLQAWSN 240
             EE+EGLGKHTDISCVTILFQD+VGGLQMRSKGG+W DI+PCEDA+VVNIGDF+QAWSN
Sbjct: 181 KVEEIEGLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSN 240

Query: 241 ERLRSAEHRVVLRRNVKRFSVAFFWSYGEEREIYAPSEVVGEGNARLYRPFSTKDYRMFR 300
           ERLRSAEHRVVLR+NVKRFS++FFWSY ++REIYAPSEVVGEGN RLYRPFS K+Y+MFR
Sbjct: 241 ERLRSAEHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFR 300

Query: 301 ATGVRDNYEKVGATVKEFAGIVG 319
           AT  R NYEKVGATVK+FAGIVG
Sbjct: 301 ATSARGNYEKVGATVKDFAGIVG 317

[Close]

BLAST of Sgr030002 vs. ExPASy TrEMBL
Match: A0A6J1CI97 (gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC111011430 PE=3 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 4.0e-131
Identity = 239/323 (73.99%), Postives = 275/323 (85.14%), Query Frame = 0


Query: 4   SETTSCVVELPVIDLSQPNLNDE--SSLSAACMEWGFFYITNHGVSMEFYRKLISLADQL 63
           SE+ S  VELPVID+ + +L++   SSLS AC EWGFFYI NHGVS EFY+KLIS+ADQL
Sbjct: 2   SESKSYTVELPVIDVYE-DLSESALSSLSEACSEWGFFYIRNHGVSKEFYQKLISIADQL 61

Query: 64  FALPQDSKTNVGISSYVPRFVASPFFESFKVSGPDFSASARALTEALFADGHRNTEFSNL 123
           F LP ++K  VG S Y+PRF+ASPF+ESFK SGPDFSASA  LTE L   G  + EFSNL
Sbjct: 62  FCLPHETKNKVGFSCYIPRFIASPFYESFKFSGPDFSASAHHLTETLL--GKHDAEFSNL 121

Query: 124 LEEYGSKMTDLSKRIVKLLLKILGENFENKFYDSEFSNCNGYLRINNYSPGDSKEE---E 183
           L+EYGSKMTD+S+RIVKLLLKILG+NFENKFYDSEFSNC+GYLR+NNYSP DS E    E
Sbjct: 122 LKEYGSKMTDISRRIVKLLLKILGDNFENKFYDSEFSNCDGYLRMNNYSPQDSNENVKIE 181

Query: 184 EVEGLGKHTDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVVVNIGDFLQAWSNERL 243
           EVEGLGKHTDISCVTILFQD VGGLQMRSK G+W DI+PCEDA+VVNIGDF+ AWSNE+L
Sbjct: 182 EVEGLGKHTDISCVTILFQDHVGGLQMRSKEGEWMDIKPCEDALVVNIGDFMHAWSNEKL 241

Query: 244 RSAEHRVVLRRN-VKRFSVAFFWSYGEEREIYAPSEVVGEGNARLYRPFSTKDYRMFRAT 303
           RSAEHRVVLR+N VKRFS+ F WS+ +++EIYAPSEVVGEGNARLY+PF TK+YR+FRAT
Sbjct: 242 RSAEHRVVLRQNSVKRFSMVFIWSFEDDKEIYAPSEVVGEGNARLYKPFLTKEYRIFRAT 301

Query: 304 --GVRDNYEKVGATVKEFAGIVG 319
             G R+NYEKVGA V +FAGIVG
Sbjct: 302 PRGARENYEKVGANVTDFAGIVG 321

[Close]

BLAST of Sgr030002 vs. ExPASy TrEMBL
Match: A0A6J1CHI8 (gibberellin 20-oxidase-like protein isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011697 PE=3 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 7.8e-111
Identity = 219/320 (68.44%), Postives = 239/320 (74.69%), Query Frame = 0


Query: 1   MSESETTSCVVELPVIDLSQPNLNDE--SSLSAACMEWGFFYITNHGVSMEFYRKLISLA 60
           MSES+T    VELPVIDLSQP L++    SL AAC EWGFFYITNHGVS EFY+KLIS+A
Sbjct: 1   MSESQT---CVELPVIDLSQP-LSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIA 60

Query: 61  DQLFALPQDSKTNVGISSYVPRFVASPFFESFKVSGPDFSASARALTEALFADGHRNTEF 120
           D+LF LPQD+K  VGISSYVPRF ASPFFESFK+SGPDFSASA+ LTEAL   GH NT+F
Sbjct: 61  DELFRLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALL--GHHNTQF 120

Query: 121 SNLLEEYGSKMTDLSKRIVKLLLKILGENFENKFYDSEFSNCNGYLRINNYSPGDSKEEE 180
                                                         RINNYSP DS   E
Sbjct: 121 ----------------------------------------------RINNYSPRDS--NE 180

Query: 181 EVEGLGKHTDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVVVNIGDFLQAWSNERL 240
           EVEGLGKHTDISCVTILFQD +GGLQMRSKGG+W DIRPCE+A+VVNIGDF+QAWSNERL
Sbjct: 181 EVEGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERL 240

Query: 241 RSAEHRVVLRRNVKRFSVAFFWSYGEEREIYAPSEVVGEGNARLYRPFSTKDYRMFRATG 300
           RSAEHRVVLR+NVKRFS+AFFWSY ++ EIYAPSEVVGEGN RLYR FSTK+YRMFRATG
Sbjct: 241 RSAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATG 266

Query: 301 VRDNYEKVGATVKEFAGIVG 319
            R  YEKVGA VKEFAGIVG
Sbjct: 301 ARGTYEKVGANVKEFAGIVG 266

[Close]

BLAST of Sgr030002 vs. ExPASy TrEMBL
Match: A0A061FZ75 (2-oxoglutarate-dependent dioxygenase OS=Theobroma cacao OX=3641 GN=TCM_014890 PE=3 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 2.7e-103
Identity = 189/320 (59.06%), Postives = 242/320 (75.62%), Query Frame = 0


Query: 1   MSESETTSCVVELPVIDLSQP-NLNDESSLSAACMEWGFFYITNHGVSMEFYRKLISLAD 60
           M E++T+   VELPV DLSQP + +  SSLS AC EWGFF ITNHGVS + YRKL SL++
Sbjct: 1   MPETKTS---VELPVFDLSQPLSPSSLSSLSLACREWGFFRITNHGVSKDLYRKLYSLSN 60

Query: 61  QLFALPQDSKTNVG----ISSYVPRFVASPFFESFKVSGPDFSASARALTEALFADGHRN 120
            LF+LP +SK  +G    + +Y P F+ASPFFES +VSGPDF ASA++  + LF     N
Sbjct: 61  HLFSLPDESKLKLGPTSSLKTYTPHFIASPFFESLRVSGPDFFASAQSSVDVLFE--QPN 120

Query: 121 TEFSNLLEEYGSKMTDLSKRIVKLLLKILGENFENKFYDSEFSNCNGYLRINNYSPGDSK 180
           +EFS +L+EYGS MT LSK IV+ +LK LGE  E K ++SEF NC+GYLRINNY+P  S 
Sbjct: 121 SEFSEVLKEYGSLMTKLSKEIVEAILKSLGEELEKKSFESEFGNCHGYLRINNYTPPKSM 180

Query: 181 EEEEVEGLGKHTDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVVVNIGDFLQAWSN 240
           +EEEV+GLG HTD+SCVTI++QD++GGLQ+RSK G W DI PCED +VVNIGD + AWSN
Sbjct: 181 KEEEVDGLGMHTDMSCVTIVYQDEIGGLQVRSKEGKWMDINPCEDTLVVNIGDLMHAWSN 240

Query: 241 ERLRSAEHRVVLRRNVKRFSVAFFWSYGEEREIYAPSEVVGEGNARLYRPFSTKDYRMFR 300
            +L S+EHRVVL+R V RFS+AFFW + +E+ I+AP+EVVGE N R+Y+PF   DY  FR
Sbjct: 241 GKLSSSEHRVVLKRIVNRFSLAFFWCFEDEKVIFAPNEVVGEDNLRIYKPFVCADYLKFR 300

Query: 301 ATGVRDNYEKVGATVKEFAG 316
            +  +  ++KVG TVK+FAG
Sbjct: 301 QSSEKGRFDKVGFTVKDFAG 315

[Close]

BLAST of Sgr030002 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 349.7 bits (896), Expect = 2.4e-96
Identity = 169/310 (54.52%), Postives = 223/310 (71.94%), Query Frame = 0


Query: 11  VELPVIDLSQP-NLNDESSLSAACMEWGFFYITNHGVSMEFYRKLISLADQLFALPQDSK 70
           ++LPV+DL+QP   +  SSLS AC EWGFFY+TNHG+S E + K+ SL+  +F  P +SK
Sbjct: 8   LQLPVLDLTQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVFKAPLESK 67

Query: 71  TNVGISSYVPRFVASPFFESFKVSGPDFSASARALTEALFADGHRNTEFSNLLEEYGSKM 130
             +G  SY PR++ASP+FES  VSGPDFS SA+A  + LF D H   E    ++EYG+KM
Sbjct: 68  LKLGPISYTPRYIASPYFESLVVSGPDFSDSAKASADVLFQD-HHKPELRETMQEYGAKM 127

Query: 131 TDLSKRIVKLLLKI-LGENFENKFYDSEFSNCNGYLRINNYSPGD--SKEEEEVEGLGKH 190
            +LSKR++K+LL + LG+    + Y ++FSNC+GYLR+ NY+P     K+EE VEGLG H
Sbjct: 128 AELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEELVEGLGMH 187

Query: 191 TDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVVVNIGDFLQAWSNERLRSAEHRVV 250
           TD+SC+TI++QD VGGLQMRSK G W DI PC D +VVNIGD +QAWSN RLRS+EHRVV
Sbjct: 188 TDMSCITIVYQDSVGGLQMRSKEGKWIDINPCNDFLVVNIGDLMQAWSNGRLRSSEHRVV 247

Query: 251 LRRNVKRFSVAFFWSYGEEREIYAPSEVVGEGNARLYRPFSTKDYRMFRATGVRDNYEKV 310
           LR+ V R S+AFF  + +E+ I AP E+VGEG  R Y+ F   +Y  FR +     +EK+
Sbjct: 248 LRKLVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQSNEEGKFEKI 307

Query: 311 GATVKEFAGI 317
           G TVK+FAG+
Sbjct: 308 GFTVKDFAGL 316

[Close]

BLAST of Sgr030002 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 317.0 bits (811), Expect = 1.7e-86
Identity = 161/322 (50.00%), Postives = 227/322 (70.50%), Query Frame = 0


Query: 11  VELPVIDLSQPNLNDES--SLSAACMEWGFFYITNHGVSMEFYRKLISLADQLFALPQDS 70
           +ELPV D+S+P L++ S  SL  AC EWGFFY+TNHGVS + Y+KL   +  +F L  + 
Sbjct: 3   LELPVFDISKP-LSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEE 62

Query: 71  KTNVGISSYVPRFVASPFFESFKVSGPDFSASARALTEALFADGHRNTEFSNLLEEYGSK 130
           K  +G S+Y PRF+ASPFFES +VSGPDF ASA++  +A F+D   + EFS L++EYG K
Sbjct: 63  KMKMGASNYTPRFIASPFFESLRVSGPDFYASAKSSVDA-FSDQATDEEFSGLMKEYGEK 122

Query: 131 MTDLSKRIVKLLLKILGENFENKFYDSEFSNCNGYLRINNYS-PGDSK-------EEEEV 190
           MT L ++I+K +L   G++  +K+Y+SEF NC+GY RINNY+ P D +       E++ +
Sbjct: 123 MTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQDLI 182

Query: 191 EGLGKHTDISCVTILFQDDVGGLQMRSKGG-DWTDIRPCEDAVVVNIGDFLQAWSNERLR 250
           EGLG HTD+SC+TI+ QDD+GGLQ+R++ G    DI P ++A+VVN+GD L AW+N RLR
Sbjct: 183 EGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLLHAWTNGRLR 242

Query: 251 SAEHRVVLRRN---VKRFSVAFFWSYGEEREIYAPSEVVG--EGNARLYRPFSTKDYRMF 310
           S++HRV+L+R      RFS+AFFW + + + ++AP EVVG  EG  R++R F   DY  F
Sbjct: 243 SSQHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEG-MRVFRSFKCGDYLRF 302

Query: 311 RATGVRDNYEKVGATVKEFAGI 317
           R +  +  +EKVG TV++FA I
Sbjct: 303 RESNEKGKFEKVGDTVEDFARI 321

[Close]

BLAST of Sgr030002 vs. TAIR 10
Match: AT4G23340.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 237.3 bits (604), Expect = 1.7e-62
Identity = 111/198 (56.06%), Postives = 147/198 (74.24%), Query Frame = 0


Query: 122 LEEYGSKMTDLSKRIVKLLLKI-LGENFENKFYDSEFSNCNGYLRINNYSPGD--SKEEE 181
           ++EYG+KM +LSKR++K+LL + LG+    + Y ++FSNC+GYLR+ NY+P     K+EE
Sbjct: 1   MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEE 60

Query: 182 EVEGLGKHTDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVVVNIGDFLQAWSNERL 241
            VEGLG HTD+SC+TI++QD VGGLQMRSK G W DI PC D +VVNIGD +QAWSN RL
Sbjct: 61  LVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCNDFLVVNIGDLMQAWSNGRL 120

Query: 242 RSAEHRVVLRRNVKRFSVAFFWSYGEEREIYAPSEVVGEGNARLYRPFSTKDYRMFRATG 301
           RS+EHRVVLR+ V R S+AFF  + +E+ I AP E+VGEG  R Y+ F   +Y  FR + 
Sbjct: 121 RSSEHRVVLRKLVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQSN 180

Query: 302 VRDNYEKVGATVKEFAGI 317
               +EK+G TVK+FAG+
Sbjct: 181 EEGKFEKIGFTVKDFAGL 198

[Close]

BLAST of Sgr030002 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 147.9 bits (372), Expect = 1.4e-35
Identity = 101/324 (31.17%), Postives = 162/324 (50.00%), Query Frame = 0


Query: 2   SESETTSCVVE-----LPVIDLSQPNLNDE-------SSLSAACMEWGFFYITNHGVSME 61
           SES T +  ++     LPVIDLS     +E         + AA  EWGFF I NHG+  +
Sbjct: 24  SESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKD 83

Query: 62  FYRKLISLADQLFALPQDSKTNVGISSYVPRFVASPFFESFKVSGPDFSASAR-ALTEA- 121
            +  ++    +LF  P   K     S             S++   P  ++ A+ +++EA 
Sbjct: 84  VFEMMLLEEKKLFDQPFSVKVRERFSDLSK--------NSYRWGNPSATSPAQYSVSEAF 143

Query: 122 --LFADGHRNTEFSNLLEEYGSKMTDLSKRIVKLLLKILGE--NFENKFYDSEFSNCNGY 181
             + ++  R ++  N L            R+ +++ +ILG+  N  ++++++ F   N +
Sbjct: 144 HIILSEVSRISDDRNNLRTIVETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSF 203

Query: 182 LRINNYSPGDSKEEEEVEGLGKHTDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVV 241
           LR+N Y P  S    EV GL  HTD S +TIL QD +GGL++ +  G W  ++PC +A+ 
Sbjct: 204 LRLNKYHP--SVFGSEVFGLVPHTDTSFLTILSQDQIGGLELEN-NGQWISVKPCLEALT 263

Query: 242 VNIGDFLQAWSNERLRSAEHRVVLRRNVKRFSVAFFWSYGEEREIYAPSEVVGEGNARLY 301
           VNIGD  QA SN   +S  HRV+   N++R S+AFF         Y  +E+   G  + Y
Sbjct: 264 VNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVC------PYLETEIDCFGYPKKY 323

Query: 302 RPFSTKDYRMFRATGVRDNYEKVG 308
           R FS ++Y+      V++  +KVG
Sbjct: 324 RRFSFREYKEQSEHDVKETGDKVG 330

[Close]

BLAST of Sgr030002 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 146.7 bits (369), Expect = 3.1e-35
Identity = 95/325 (29.23%), Postives = 160/325 (49.23%), Query Frame = 0


Query: 4   SETTSCVVELPVIDLSQPNLN--------DESSLSAACMEWGFFYITNHGVSMEFYRKLI 63
           SET    + LPVID S  NL           + +  A  ++G+F  +   +  E  + + 
Sbjct: 3   SETPLLPLRLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVF 62

Query: 64  SLADQLFALPQDSK-TNVG---ISSYVPRFVASPFFESFKVSGPDFSASARALTEALFAD 123
              ++LF LP  +K  NV       YV ++   P +ES  +   D +    A TE L+  
Sbjct: 63  GALEELFDLPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWPQ 122

Query: 124 GHRNTEFSNLLEEYGSKMTDLSKRIVKLLLKILGENFENKFYDSEFSNCNGYLRINNYSP 183
           G  N  FS  ++ +  K+++L   I +++++  G    +K+ D    + N  LR+  Y  
Sbjct: 123 G--NISFSTTIQSFSKKLSELDITIRRMIMESFG---LDKYIDEHLHSTNYLLRVMKYKG 182

Query: 184 GDSKEEEEVEGLGKHTDISCVTILFQDDVGGLQMRSKGGDWTDIRPCEDAVVVNIGDFLQ 243
            D+  EE   GL  HTD + VTIL+Q+ V GL++++K  +W  ++P +D+  V IGD L 
Sbjct: 183 PDT--EETKVGLNAHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPTQDSFTVMIGDSLY 242

Query: 244 AWSNERLRSAEHRVVLRRNVKRFSVAFFWSYGEEREIYAPSEVVGEGNARLYRPFSTKDY 303
           A  N RL S  HRV++     R+S+  F        + +P E+V E + RL++PF   ++
Sbjct: 243 ALLNGRLHSPYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEF 302

Query: 304 RMFRATGVRDNYEKVGATVKEFAGI 317
             F  T   +  ++  + +K + GI
Sbjct: 303 LQFYYT---EAGQRSQSALKTYCGI 317

The following BLAST results are available for this feature:

BLAST of Sgr030002 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141254.1	6.0e-142	80.94	gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia]	[more]
XP_022141253.1	1.4e-135	76.78	gibberellin 20-oxidase-like protein [Momordica charantia]	[more]
XP_022140867.1	8.2e-131	73.99	gibberellin 20-oxidase-like protein [Momordica charantia]	[more]
XP_022141255.1	1.6e-110	68.44	gibberellin 20-oxidase-like protein isoform X2 [Momordica charantia]	[more]
XP_031265650.1	4.1e-106	60.44	gibberellin 20-oxidase-like protein [Pistacia vera]	[more]

BLAST of Sgr030002 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KBY0	2.4e-85	50.00	Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310...	[more]
Q9C6I4	1.9e-34	31.17	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ...	[more]
O49561	1.5e-31	32.38	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
Q9C899	5.9e-31	35.81	Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 S...	[more]
Q39103	2.5e-29	29.01	Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 ...	[more]

BLAST of Sgr030002 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CI26	1.7e-142	81.25	gibberellin 20-oxidase-like protein isoform X1 OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1CJY7	7.0e-136	76.78	gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC1110116...	[more]
A0A6J1CI97	4.0e-131	73.99	gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC1110114...	[more]
A0A6J1CHI8	7.8e-111	68.44	gibberellin 20-oxidase-like protein isoform X2 OS=Momordica charantia OX=3673 GN...	[more]
A0A061FZ75	2.7e-103	59.06	2-oxoglutarate-dependent dioxygenase OS=Theobroma cacao OX=3641 GN=TCM_014890 PE...	[more]

BLAST of Sgr030002 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23340.1	2.4e-96	54.52	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51310.1	1.7e-86	50.00	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G23340.2	1.7e-62	56.06	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G50960.1	1.4e-35	31.17	gibberellin 2-oxidase 7	[more]
AT1G52820.1	3.1e-35	29.23	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 29..46 score: 53.54 coord: 250..268 score: 35.89
None	No IPR available	PANTHER	PTHR47990:SF9	GIBBERELLIN 20-OXIDASE-LIKE PROTEIN	coord: 8..311
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 8..311
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 10..301
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 13..75 e-value: 1.5E-14 score: 54.7
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 165..259 e-value: 1.5E-23 score: 83.2
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..294 e-value: 3.4E-70 score: 239.2
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 160..262 score: 11.607044

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr030002.1	Sgr030002.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding