Sgr023216 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr023216
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptioncalcium-dependent protein kinase 8-like
Locationtig00000892: 1095395 .. 1098292 (+)
RNA-Seq ExpressionSgr023216
SyntenySgr023216
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTAATTGCTGTGCTACTCCTGGCACTCCTTCGGCTAAGACTAAAGTTAAGCACAAGAAGAAAGCTAATCCATTTTCTTCTAACGATGGCGGGAATAATGTGAATGGTGCTGGCAACAAGCTTCGGGTCTTGAAAGATCCCACTGGTCATGACATTTTGGCTCAGTATGATCTCGGTCGCGAGCTCGGTAGAGGTGAATTTGGAGTGACATATTTATGCACAGATACTTCTACAGGTGAAAAGTTTGCCTGTAAATCTATATCAAAGAAGAAACTTAGGACTGCTATTGATATTGAGGATGTGCGAAGAGAGGTTCAAATAATGAAGCATTTGCCCAAGCATTCGAATATTGTTACACTGAAGGACACTTATGAGGATGATCAGGCAGTTCATATTGTGATGGAACTGTGCGAGGGTGGGGAGTTGTTTGACCGAATTGTTGCAAGGGGGCATTACACAGAGCGTGCAGCAGCTGCTGTTATGCGGACAATTGTTGAAGTTGTTCAGGTGAAAATAAAATGTTGGTTAACTTAAACATGGAAGATTTTTTTTTTTTTTCTAGTTTAGTTTAGTTAGACCATGTCTTAATCTATGTGAAACTGTGTAATTGTTAATATCAACGCTTTTGGCATATTTATGCTGATTGTATCTGCCAGATGATTTCAACATCTTTGTTCTTGAGAAGTAATCTTGGTTATTTACATGCATTTTACGTTATAGGTTTGTCATAAACATGGAGTGATGCATCGTGATCTCAAACCTGAGAATTTTCTTTTTGCAAATAAGAAGGAATCATCCCCATTGAAGGCAATTGATTTTGGGCTGTCTATCACTTTTAAACCAGGTATTGCGGCTTGTATTCAATCGACATGTGAGATTCATGTTATCATTTTGTTGTGCATTCTCGTTTTGTTTGTGTGGTTGCACTTGCCACGCCTTTCTATTTGTCATGTCATGAAAAATCCTACTATACATTAATGTTTCTATTGGATTAGGTGAACAATTTAATGAAATAGTAGGCAGTCCTTATTACATGGCTCCAGAGGTCCTGAAAAGGAATTATGGTCCAGAAGTTGATGTTTGGAGCGCCGGGGTTATCCTCTATATTTTACTCTGTGGCATCCCACCATTTTGGGCAGGTTTGAATTTTTGTTCTCTACAGTCAGTTCATGTCTTTAATAGACCATCTTTTTTTTATCAGAAATGGTCATATCTGGATTATCTGCAAAGAACAGTGAATCCGTCTACCATGTTGTTTAAAAATAAGTTCTTATTACTTGTTGCTAGGATCTTCCACACAACTAGCCCGTTAGTGTAATTTTTGCAATTCTTTTTTCAAATTTCCTCCTCACAAGTTGCATTCCAATTTTCATGTAGAAACTGAACAGGGTGTAGCACAGGCAATTATTCGCTCAGTTATTGATTTTAGGAGAGACCCTTGGCCAAGAGTCTCTGAAAATGCAAAGGACCTCGTAAGGAAAATGCTTAATCCTGATCCAAAGCGGCGCCTTACTGCTCTGGAAGTTCTAGGTAATATGTGCACTGATTTCTCATGCATTCTGAGATCTAGCTCTTATAGTTTAAGAATTTGCACACAATACCTGAAATATCGCTTTGTGCAGACCATCCGTGGTTACAAAATGCTAAGAAGGCCCCAAATGTTCCTTTAGGTGAGACAGTAAAAGCGAGGCTCAAGCAATTTTCTGTGATGAACAAGCTCAAGAAACGAGCTCTTAGGGTAATAACAAAAGTCATTCGGGGTGTTTAATAGATTACTCGAGTTATAGATGACACTCCATTTCATTATTGTTCTTAGGTAATTGCTGAGCATTTATCAGTGGAGGAAGTGGCTGGCATAAAGGAGGCATTTGATATGATGGACGTTGAGAAGAGAGGCAAGATAAATCTCGAGGAACTGCGAAATGGGATGCAAAAGCTTGGACAACAGATTCCAGACACAGATCTTCAGATACTTATGGAAGCAGTATGTACAGTTCTCTGTTTTTTTTTTTGGTACTTTTGTATCCTTCCGCGGGTGTGCATTGGATTTGCAATCTTCCTTCTTTCTTGGAATCGTGTACTTTTTTCAATAAGTGAACGTGTATGGAGGCTGATATTCTCTAAAAGGAAACCGAAAAATTGGGAAGGATGTTAGAGGCAATAAAAGAAAAGAGTATAAATGCGCGGATTCGTTATGACGTCTCGTCTGTGATTCAAAGGCAGTTCTTTTTATAGAAAACTCACACTTGTCAGTCTGTTCTACGAAGTAGTAATCAGACTGAGAGGCAGAATTACTGACTCAACTTTGCTTTATACAGGCTGGTGTTCATGGTGATGGAACTCTAAACTATGGAGAGTTTGTTGCAGTTTCGATCCACTTTAAAAGGATGGCAAATGATGAGCACCTACATAAAGCTTTTGCATTCTTTGATCAAAATCAGAGTGGTTACATTGAGATTGAAGAGCTTCGAGCTGTGCTAAACGCTGACGAAGAAACAAACAGTGAGGATGTCATCAGTGCCATTATGCATGATGTCGACACAGATAAGGTTAGCATCGCGGTTGTGTTTAGGAATAAATAGCTCATGTCCAGTTTTTAGTAACACGCACAAACTTGATCAACCTCGAAACTCTTCGTAATTCTAGTTCCTGCTAGGAAAACATCTGAAAGTTGTTCAGCTCCATATATGTTGATATAGATGTGGAGACTTTTAAGCCAGATCCAACCCTTTTATTCTTCTTCTGTAGGACGGATGCATAAGCTATGAAGAGTTTGCAGCCATGATGAAGGCGGGCACCGATTGGAGAAAAGCATCGAGGCAATATTCACGGGAAAGATTCAACAGCCTGAGCTTGAAGTTGTTCAGAGAAGGGTCATTGAATCTTACTAGTTAA

mRNA sequence

ATGGGTAATTGCTGTGCTACTCCTGGCACTCCTTCGGCTAAGACTAAAGTTAAGCACAAGAAGAAAGCTAATCCATTTTCTTCTAACGATGGCGGGAATAATGTGAATGGTGCTGGCAACAAGCTTCGGGTCTTGAAAGATCCCACTGGTCATGACATTTTGGCTCAGTATGATCTCGGTCGCGAGCTCGGTAGAGGTGAATTTGGAGTGACATATTTATGCACAGATACTTCTACAGGTGAAAAGTTTGCCTGTAAATCTATATCAAAGAAGAAACTTAGGACTGCTATTGATATTGAGGATGTGCGAAGAGAGGTTCAAATAATGAAGCATTTGCCCAAGCATTCGAATATTGTTACACTGAAGGACACTTATGAGGATGATCAGGCAGTTCATATTGTGATGGAACTGTGCGAGGGTGGGGAGTTGTTTGACCGAATTGTTGCAAGGGGGCATTACACAGAGCGTGCAGCAGCTGCTGTTATGCGGACAATTGTTGAAGTTGTTCAGGTTTGTCATAAACATGGAGTGATGCATCGTGATCTCAAACCTGAGAATTTTCTTTTTGCAAATAAGAAGGAATCATCCCCATTGAAGGCAATTGATTTTGGGCTGTCTATCACTTTTAAACCAGGTGAACAATTTAATGAAATAGTAGGCAGTCCTTATTACATGGCTCCAGAGGTCCTGAAAAGGAATTATGGTCCAGAAGTTGATGTTTGGAGCGCCGGGGTTATCCTCTATATTTTACTCTGTGGCATCCCACCATTTTGGGCAGAAACTGAACAGGGTGTAGCACAGGCAATTATTCGCTCAGTTATTGATTTTAGGAGAGACCCTTGGCCAAGAGTCTCTGAAAATGCAAAGGACCTCGTAAGGAAAATGCTTAATCCTGATCCAAAGCGGCGCCTTACTGCTCTGGAAGTTCTAGACCATCCGTGGTTACAAAATGCTAAGAAGGCCCCAAATGTTCCTTTAGGTGAGACAGTAAAAGCGAGGCTCAAGCAATTTTCTGTGATGAACAAGCTCAAGAAACGAGCTCTTAGGGTAATTGCTGAGCATTTATCAGTGGAGGAAGTGGCTGGCATAAAGGAGGCATTTGATATGATGGACGTTGAGAAGAGAGGCAAGATAAATCTCGAGGAACTGCGAAATGGGATGCAAAAGCTTGGACAACAGATTCCAGACACAGATCTTCAGATACTTATGGAAGCAGCTGGTGTTCATGGTGATGGAACTCTAAACTATGGAGAGTTTGTTGCAGTTTCGATCCACTTTAAAAGGATGGCAAATGATGAGCACCTACATAAAGCTTTTGCATTCTTTGATCAAAATCAGAGTGGTTACATTGAGATTGAAGAGCTTCGAGCTGTGCTAAACGCTGACGAAGAAACAAACAGTGAGGATGTCATCAGTGCCATTATGCATGATGTCGACACAGATAAGGACGGATGCATAAGCTATGAAGAGTTTGCAGCCATGATGAAGGCGGGCACCGATTGGAGAAAAGCATCGAGGCAATATTCACGGGAAAGATTCAACAGCCTGAGCTTGAAGTTGTTCAGAGAAGGGTCATTGAATCTTACTAGTTAA

Coding sequence (CDS)

ATGGGTAATTGCTGTGCTACTCCTGGCACTCCTTCGGCTAAGACTAAAGTTAAGCACAAGAAGAAAGCTAATCCATTTTCTTCTAACGATGGCGGGAATAATGTGAATGGTGCTGGCAACAAGCTTCGGGTCTTGAAAGATCCCACTGGTCATGACATTTTGGCTCAGTATGATCTCGGTCGCGAGCTCGGTAGAGGTGAATTTGGAGTGACATATTTATGCACAGATACTTCTACAGGTGAAAAGTTTGCCTGTAAATCTATATCAAAGAAGAAACTTAGGACTGCTATTGATATTGAGGATGTGCGAAGAGAGGTTCAAATAATGAAGCATTTGCCCAAGCATTCGAATATTGTTACACTGAAGGACACTTATGAGGATGATCAGGCAGTTCATATTGTGATGGAACTGTGCGAGGGTGGGGAGTTGTTTGACCGAATTGTTGCAAGGGGGCATTACACAGAGCGTGCAGCAGCTGCTGTTATGCGGACAATTGTTGAAGTTGTTCAGGTTTGTCATAAACATGGAGTGATGCATCGTGATCTCAAACCTGAGAATTTTCTTTTTGCAAATAAGAAGGAATCATCCCCATTGAAGGCAATTGATTTTGGGCTGTCTATCACTTTTAAACCAGGTGAACAATTTAATGAAATAGTAGGCAGTCCTTATTACATGGCTCCAGAGGTCCTGAAAAGGAATTATGGTCCAGAAGTTGATGTTTGGAGCGCCGGGGTTATCCTCTATATTTTACTCTGTGGCATCCCACCATTTTGGGCAGAAACTGAACAGGGTGTAGCACAGGCAATTATTCGCTCAGTTATTGATTTTAGGAGAGACCCTTGGCCAAGAGTCTCTGAAAATGCAAAGGACCTCGTAAGGAAAATGCTTAATCCTGATCCAAAGCGGCGCCTTACTGCTCTGGAAGTTCTAGACCATCCGTGGTTACAAAATGCTAAGAAGGCCCCAAATGTTCCTTTAGGTGAGACAGTAAAAGCGAGGCTCAAGCAATTTTCTGTGATGAACAAGCTCAAGAAACGAGCTCTTAGGGTAATTGCTGAGCATTTATCAGTGGAGGAAGTGGCTGGCATAAAGGAGGCATTTGATATGATGGACGTTGAGAAGAGAGGCAAGATAAATCTCGAGGAACTGCGAAATGGGATGCAAAAGCTTGGACAACAGATTCCAGACACAGATCTTCAGATACTTATGGAAGCAGCTGGTGTTCATGGTGATGGAACTCTAAACTATGGAGAGTTTGTTGCAGTTTCGATCCACTTTAAAAGGATGGCAAATGATGAGCACCTACATAAAGCTTTTGCATTCTTTGATCAAAATCAGAGTGGTTACATTGAGATTGAAGAGCTTCGAGCTGTGCTAAACGCTGACGAAGAAACAAACAGTGAGGATGTCATCAGTGCCATTATGCATGATGTCGACACAGATAAGGACGGATGCATAAGCTATGAAGAGTTTGCAGCCATGATGAAGGCGGGCACCGATTGGAGAAAAGCATCGAGGCAATATTCACGGGAAAGATTCAACAGCCTGAGCTTGAAGTTGTTCAGAGAAGGGTCATTGAATCTTACTAGTTAA

Protein sequence

MGNCCATPGTPSAKTKVKHKKKANPFSSNDGGNNVNGAGNKLRVLKDPTGHDILAQYDLGRELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHSNIVTLKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHGVMHRDLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEVDVWSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDPKRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEVAGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQILMEAAGVHGDGTLNYGEFVAVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAIMHDVDTDKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNLTS
Homology
BLAST of Sgr023216 vs. NCBI nr
Match: XP_038901584.1 (calcium-dependent protein kinase 8-like [Benincasa hispida] >XP_038901585.1 calcium-dependent protein kinase 8-like [Benincasa hispida])

HSP 1 Score: 996.9 bits (2576), Expect = 6.7e-287
Identity = 501/531 (94.35%), Postives = 510/531 (96.05%), Query Frame = 0

Query: 1   MGNCCATPGTPSAKTKVKHKKKANPFSSNDGGNNVNGAG-NKLRVLKDPTGHDILAQYDL 60
           MGNCCATPGTPSAK K KHKKKA PFS+ DGG NVNG+G NKL+VL DPTGHDI A YDL
Sbjct: 1   MGNCCATPGTPSAKPKGKHKKKAKPFSAKDGGKNVNGSGNNKLQVLSDPTGHDISAHYDL 60

Query: 61  GRELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHSNIV 120
           GRELGRGEFGVTYLCTDT+TGEKFACKSISKKKLRTA+DIEDVRREVQIMKHLPKH NIV
Sbjct: 61  GRELGRGEFGVTYLCTDTNTGEKFACKSISKKKLRTAVDIEDVRREVQIMKHLPKHPNIV 120

Query: 121 TLKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHGVMH 180
           TLKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAA VMRTIVEVVQ CHKHGVMH
Sbjct: 121 TLKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAVVMRTIVEVVQACHKHGVMH 180

Query: 181 RDLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEVD 240
           RDLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEVD
Sbjct: 181 RDLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEVD 240

Query: 241 VWSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPD 300
           VWSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPD
Sbjct: 241 VWSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPD 300

Query: 301 PKRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEE 360
           PKRRLTA EVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEE
Sbjct: 301 PKRRLTAQEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEE 360

Query: 361 VAGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQILMEAAGVHGDGTLNYGEF 420
           VAGIKEAFDMMDVEKRGKINLEELR+G+QKLGQQIP+TDLQILMEAAGV  DGTLNYGEF
Sbjct: 361 VAGIKEAFDMMDVEKRGKINLEELRSGLQKLGQQIPETDLQILMEAAGVQDDGTLNYGEF 420

Query: 421 VAVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAIMHDVD 480
           VAVSIH KRMANDEHLHKAFAFFDQNQSG+IEIEELRAVL  DEETN EDVISAIMHDVD
Sbjct: 421 VAVSIHLKRMANDEHLHKAFAFFDQNQSGFIEIEELRAVLLDDEETNIEDVISAIMHDVD 480

Query: 481 TDKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNLTS 531
           TDKDG ISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSL LTS
Sbjct: 481 TDKDGRISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLKLTS 531

BLAST of Sgr023216 vs. NCBI nr
Match: XP_022148790.1 (calcium-dependent protein kinase 8-like [Momordica charantia])

HSP 1 Score: 974.2 bits (2517), Expect = 4.6e-280
Identity = 489/534 (91.57%), Postives = 504/534 (94.38%), Query Frame = 0

Query: 1   MGNCCATPGTPSA----KTKVKHKKKANPFSSNDGGNNVNGAGNKLRVLKDPTGHDILAQ 60
           MGNCCATPGTPSA    KTK KHKKK N   ++D    VNGAGNKLRVLK+PTGHDI A 
Sbjct: 1   MGNCCATPGTPSAKPKTKTKGKHKKKGNRSPTDDNRKQVNGAGNKLRVLKEPTGHDISAH 60

Query: 61  YDLGRELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHS 120
           YDLGRELGRGEFGVTYLCTDT+TGEKFACKSISKKKLRTA+DIEDVRREVQIMKHLPKH 
Sbjct: 61  YDLGRELGRGEFGVTYLCTDTNTGEKFACKSISKKKLRTAVDIEDVRREVQIMKHLPKHP 120

Query: 121 NIVTLKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHG 180
           NIVTLKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAA VMRTIVEVVQVCHKHG
Sbjct: 121 NIVTLKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAVVMRTIVEVVQVCHKHG 180

Query: 181 VMHRDLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGP 240
           VMHRDLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYG 
Sbjct: 181 VMHRDLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGQ 240

Query: 241 EVDVWSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKML 300
           EVDVWSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPR+S+NAKDLVRKML
Sbjct: 241 EVDVWSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRISDNAKDLVRKML 300

Query: 301 NPDPKRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLS 360
           NPDPKRRLTA EVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLS
Sbjct: 301 NPDPKRRLTAQEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLS 360

Query: 361 VEEVAGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQILMEAAGVHGDGTLNY 420
           VEEVAGIKEAFD MDVEK+GKI+LEELR G+QKLG QIPD+DLQILMEAAGVH DGTLNY
Sbjct: 361 VEEVAGIKEAFDTMDVEKKGKIHLEELRTGLQKLGHQIPDSDLQILMEAAGVHDDGTLNY 420

Query: 421 GEFVAVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAIMH 480
           GEFVA+SIH KRMANDEHLHKAF+FFDQ+QSGYIEIEELRAVLN +EETNSEDVI AIMH
Sbjct: 421 GEFVAISIHLKRMANDEHLHKAFSFFDQDQSGYIEIEELRAVLNDEEETNSEDVIIAIMH 480

Query: 481 DVDTDKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNLTS 531
           DVDTDKDG ISYEEFA MMKAGTDWRKASRQYSRERFNSLSLKLFREGSL  TS
Sbjct: 481 DVDTDKDGRISYEEFAVMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLKHTS 534

BLAST of Sgr023216 vs. NCBI nr
Match: XP_011657639.1 (calcium-dependent protein kinase 8 [Cucumis sativus] >KGN48128.1 hypothetical protein Csa_003146 [Cucumis sativus])

HSP 1 Score: 971.1 bits (2509), Expect = 3.9e-279
Identity = 487/530 (91.89%), Postives = 501/530 (94.53%), Query Frame = 0

Query: 1   MGNCCATPGTPSAKTKVKHKKKANPFSSNDGGNNVNGAGNKLRVLKDPTGHDILAQYDLG 60
           MGNCCATP TPSAK K K+ KKANPF + DG  N NG GNKL+VL DPTGH+I A YDLG
Sbjct: 1   MGNCCATPSTPSAKPKGKN-KKANPFPAKDGEKNANGHGNKLQVLSDPTGHNISAHYDLG 60

Query: 61  RELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHSNIVT 120
           RELGRGEFGVTYLCTDT+TGEKFACKSISKKKLRTA+DIEDVRREVQIMKHLP H N+V 
Sbjct: 61  RELGRGEFGVTYLCTDTNTGEKFACKSISKKKLRTAVDIEDVRREVQIMKHLPNHPNVVR 120

Query: 121 LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHGVMHR 180
           LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAA VMRTIVEVVQ CHKHGVMHR
Sbjct: 121 LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAVVMRTIVEVVQACHKHGVMHR 180

Query: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEVDV 240
           DLKPENFLFANKKESSPLKAIDFGLSITFKPG+ FNEIVGSPYYMAPEVLKRNYGPEVDV
Sbjct: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKPGDLFNEIVGSPYYMAPEVLKRNYGPEVDV 240

Query: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP 300
           WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP
Sbjct: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP 300

Query: 301 KRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360
           KRRLTA EVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV
Sbjct: 301 KRRLTAQEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360

Query: 361 AGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQILMEAAGVHGDGTLNYGEFV 420
           AGIKEAFDMMDVEKRGKINLEELR+G+QKLGQQIP+ DLQILMEAAGV  DG LNYGEFV
Sbjct: 361 AGIKEAFDMMDVEKRGKINLEELRSGLQKLGQQIPEADLQILMEAAGVQDDGALNYGEFV 420

Query: 421 AVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAIMHDVDT 480
           AVSIH KRMANDEHLHKAFAFFDQNQSG+IEIEELRAVL  DEETNSEDV++AIMHDVDT
Sbjct: 421 AVSIHLKRMANDEHLHKAFAFFDQNQSGFIEIEELRAVLLNDEETNSEDVVNAIMHDVDT 480

Query: 481 DKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNLTS 531
           DKDG ISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSL LTS
Sbjct: 481 DKDGRISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLKLTS 529

BLAST of Sgr023216 vs. NCBI nr
Match: XP_022944585.1 (calcium-dependent protein kinase 8-like [Cucurbita moschata])

HSP 1 Score: 969.9 bits (2506), Expect = 8.7e-279
Identity = 484/530 (91.32%), Postives = 504/530 (95.09%), Query Frame = 0

Query: 1   MGNCCATPGTPSAKTKVKHKKKANPFSSNDGGNNVNGAGNKLRVLKDPTGHDILAQYDLG 60
           MGNCCATP +PS K K KHKKK+ PFS     +NV GA NKLRVLK PTGHDI A YDLG
Sbjct: 1   MGNCCATPVSPSGKPKGKHKKKSFPFSGKGSRSNVVGADNKLRVLKAPTGHDISAHYDLG 60

Query: 61  RELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHSNIVT 120
           RELGRGEFGVTYLCTDT+TGE FACKSISKKKLRTA+DIEDVRREVQIMKHLPKH NIVT
Sbjct: 61  RELGRGEFGVTYLCTDTNTGESFACKSISKKKLRTAVDIEDVRREVQIMKHLPKHPNIVT 120

Query: 121 LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHGVMHR 180
           LKDTYEDD+AVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVE+V+ CHKHGVMHR
Sbjct: 121 LKDTYEDDEAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEIVEACHKHGVMHR 180

Query: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEVDV 240
           DLKPENFLFANKKESSPLKAIDFGLSITFK GEQFNEIVGSPYYMAPEVLK+NYGPEVDV
Sbjct: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKRGEQFNEIVGSPYYMAPEVLKKNYGPEVDV 240

Query: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP 300
           WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKML+PDP
Sbjct: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLDPDP 300

Query: 301 KRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360
           KRRLTA EVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV
Sbjct: 301 KRRLTAQEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360

Query: 361 AGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQILMEAAGVHGDGTLNYGEFV 420
           AGIK+AF+MMDVEKRGKINLEELR+G+QKLGQQIP+ DLQILMEAAGVH DGTLNYGEFV
Sbjct: 361 AGIKDAFEMMDVEKRGKINLEELRSGLQKLGQQIPEADLQILMEAAGVHDDGTLNYGEFV 420

Query: 421 AVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAIMHDVDT 480
           A+SIH +RMANDEHLHKAFAFFDQNQSG+IEIEELRAVLN DEETNSEDVISAIMHDVDT
Sbjct: 421 ALSIHLRRMANDEHLHKAFAFFDQNQSGFIEIEELRAVLN-DEETNSEDVISAIMHDVDT 480

Query: 481 DKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNLTS 531
           DKDGCISYEEFA MMKAGTDWRKASRQYSRERFN+LSLKLFREGS NLT+
Sbjct: 481 DKDGCISYEEFAGMMKAGTDWRKASRQYSRERFNNLSLKLFREGSFNLTN 529

BLAST of Sgr023216 vs. NCBI nr
Match: KAG7010274.1 (Calcium-dependent protein kinase 8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 968.4 bits (2502), Expect = 2.5e-278
Identity = 483/530 (91.13%), Postives = 504/530 (95.09%), Query Frame = 0

Query: 1   MGNCCATPGTPSAKTKVKHKKKANPFSSNDGGNNVNGAGNKLRVLKDPTGHDILAQYDLG 60
           MGNCCATP +PS K K KHKKK+ PFS     +NV GA NKL+VLK PTGHDI A YDLG
Sbjct: 20  MGNCCATPVSPSGKPKGKHKKKSFPFSGKGSRSNVVGADNKLQVLKAPTGHDISAHYDLG 79

Query: 61  RELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHSNIVT 120
           RELGRGEFGVTYLCTDT+TGE FACKSISKKKLRTA+DIEDVRREVQIMKHLPKH NIVT
Sbjct: 80  RELGRGEFGVTYLCTDTNTGESFACKSISKKKLRTAVDIEDVRREVQIMKHLPKHPNIVT 139

Query: 121 LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHGVMHR 180
           LKDTYEDD+AVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVE+V+ CHKHGVMHR
Sbjct: 140 LKDTYEDDEAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEIVEACHKHGVMHR 199

Query: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEVDV 240
           DLKPENFLFANKKESSPLKAIDFGLSITFK GEQFNEIVGSPYYMAPEVLK+NYGPEVDV
Sbjct: 200 DLKPENFLFANKKESSPLKAIDFGLSITFKRGEQFNEIVGSPYYMAPEVLKKNYGPEVDV 259

Query: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP 300
           WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKML+PDP
Sbjct: 260 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLDPDP 319

Query: 301 KRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360
           KRRLTA EVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV
Sbjct: 320 KRRLTAQEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 379

Query: 361 AGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQILMEAAGVHGDGTLNYGEFV 420
           AGIK+AF+MMDVEKRGKINLEELR+G+QKLGQQIP+ DLQILMEAAGVH DGTLNYGEFV
Sbjct: 380 AGIKDAFEMMDVEKRGKINLEELRSGLQKLGQQIPEADLQILMEAAGVHDDGTLNYGEFV 439

Query: 421 AVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAIMHDVDT 480
           A+SIH +RMANDEHLHKAFAFFDQNQSG+IEIEELRAVLN DEETNSEDVISAIMHDVDT
Sbjct: 440 ALSIHLRRMANDEHLHKAFAFFDQNQSGFIEIEELRAVLN-DEETNSEDVISAIMHDVDT 499

Query: 481 DKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNLTS 531
           DKDGCISYEEFA MMKAGTDWRKASRQYSRERFN+LSLKLFREGS NLT+
Sbjct: 500 DKDGCISYEEFAGMMKAGTDWRKASRQYSRERFNNLSLKLFREGSFNLTN 548

BLAST of Sgr023216 vs. ExPASy Swiss-Prot
Match: Q42438 (Calcium-dependent protein kinase 8 OS=Arabidopsis thaliana OX=3702 GN=CPK8 PE=1 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 2.8e-256
Identity = 439/529 (82.99%), Postives = 479/529 (90.55%), Query Frame = 0

Query: 1   MGNCCATPGTPSAKTKVKHKKKANPFSSNDGGNNVNGAGNKLRVLKDPTGHDILAQYDLG 60
           MGNCCA+PG+ +   K K K K+NPF S     N +G G KL VLKDPTGHDI   YDLG
Sbjct: 1   MGNCCASPGSETGSKKGKPKIKSNPFYSEAYTTNGSGTGFKLSVLKDPTGHDISLMYDLG 60

Query: 61  RELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHSNIVT 120
           RE+GRGEFG+TYLCTD  TGEK+ACKSISKKKLRTA+DIEDVRREV+IMKH+P+H NIV+
Sbjct: 61  REVGRGEFGITYLCTDIKTGEKYACKSISKKKLRTAVDIEDVRREVEIMKHMPRHPNIVS 120

Query: 121 LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHGVMHR 180
           LKD +EDD AVHIVMELCEGGELFDRIVARGHYTERAAAAVM+TI+EVVQ+CHKHGVMHR
Sbjct: 121 LKDAFEDDDAVHIVMELCEGGELFDRIVARGHYTERAAAAVMKTILEVVQICHKHGVMHR 180

Query: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEVDV 240
           DLKPENFLFANKKE+S LKAIDFGLS+ FKPGE FNEIVGSPYYMAPEVL+RNYGPEVD+
Sbjct: 181 DLKPENFLFANKKETSALKAIDFGLSVFFKPGEGFNEIVGSPYYMAPEVLRRNYGPEVDI 240

Query: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP 300
           WSAGVILYILLCG+PPFWAETEQGVAQAIIRSVIDF+RDPWPRVSE AKDLVRKML PDP
Sbjct: 241 WSAGVILYILLCGVPPFWAETEQGVAQAIIRSVIDFKRDPWPRVSETAKDLVRKMLEPDP 300

Query: 301 KRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360
           K+RL+A +VL+H W+QNAKKAPNV LGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV
Sbjct: 301 KKRLSAAQVLEHSWIQNAKKAPNVSLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360

Query: 361 AGIKEAFDMMDVEKRGKINLEELRNGMQKLG-QQIPDTDLQILMEAAGVHGDGTLNYGEF 420
           AGIKEAF+MMD +K GKINLEEL+ G+ KLG QQIPDTDLQILMEAA V GDGTLNYGEF
Sbjct: 361 AGIKEAFEMMDSKKTGKINLEELKFGLHKLGQQQIPDTDLQILMEAADVDGDGTLNYGEF 420

Query: 421 VAVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAIMHDVD 480
           VAVS+H K+MANDEHLHKAF+FFDQNQS YIEIEELR  LN + +TNSE+V++AIM DVD
Sbjct: 421 VAVSVHLKKMANDEHLHKAFSFFDQNQSDYIEIEELREALNDEVDTNSEEVVAAIMQDVD 480

Query: 481 TDKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNL 529
           TDKDG ISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKL REGSL L
Sbjct: 481 TDKDGRISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLMREGSLQL 529

BLAST of Sgr023216 vs. ExPASy Swiss-Prot
Match: Q38873 (Calcium-dependent protein kinase 7 OS=Arabidopsis thaliana OX=3702 GN=CPK7 PE=2 SV=1)

HSP 1 Score: 872.5 bits (2253), Expect = 2.5e-252
Identity = 435/531 (81.92%), Postives = 478/531 (90.02%), Query Frame = 0

Query: 1   MGNCCATPGTPSAKTKV-KHKKKANPFSSND-GGNNVNGAGNKLRVLKDPTGHDILAQYD 60
           MGNCC  P + + ++K  K K K NPF SN+    + +GAG KL VLKDPTGHDI  QYD
Sbjct: 1   MGNCCGNPSSATNQSKQGKPKNKNNPFYSNEYATTDRSGAGFKLSVLKDPTGHDISLQYD 60

Query: 61  LGRELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHSNI 120
           LGRE+GRGEFG+TYLCTD  TGEK+ACKSISKKKLRTA+DIEDVRREV+IMKH+PKH N+
Sbjct: 61  LGREVGRGEFGITYLCTDKETGEKYACKSISKKKLRTAVDIEDVRREVEIMKHMPKHPNV 120

Query: 121 VTLKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHGVM 180
           V+LKD++EDD AVHIVMELCEGGELFDRIVARGHYTERAAAAVM+TIVEVVQ+CHK GVM
Sbjct: 121 VSLKDSFEDDDAVHIVMELCEGGELFDRIVARGHYTERAAAAVMKTIVEVVQICHKQGVM 180

Query: 181 HRDLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEV 240
           HRDLKPENFLFANKKE+S LKAIDFGLS+ FKPGEQFNEIVGSPYYMAPEVL+RNYGPE+
Sbjct: 181 HRDLKPENFLFANKKETSALKAIDFGLSVFFKPGEQFNEIVGSPYYMAPEVLRRNYGPEI 240

Query: 241 DVWSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNP 300
           DVWSAGVILYILLCG+PPFWAETEQGVAQAIIRSVIDF+RDPWPRVS++AKDLVRKML P
Sbjct: 241 DVWSAGVILYILLCGVPPFWAETEQGVAQAIIRSVIDFKRDPWPRVSDSAKDLVRKMLEP 300

Query: 301 DPKRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVE 360
           DPK+RLTA +VL+H W+ NAKKAPNV LGETVKARLKQFSVMNKLKKRALRVIAEHLSVE
Sbjct: 301 DPKKRLTAAQVLEHTWILNAKKAPNVSLGETVKARLKQFSVMNKLKKRALRVIAEHLSVE 360

Query: 361 EVAGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQILMEAAGVHGDGTLNYGE 420
           E AGIKEAF+MMDV KRGKINLEEL+ G+QK GQQI DTDLQILMEA  V GDGTLNY E
Sbjct: 361 EAAGIKEAFEMMDVNKRGKINLEELKYGLQKAGQQIADTDLQILMEATDVDGDGTLNYSE 420

Query: 421 FVAVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNAD-EETNSEDVISAIMHD 480
           FVAVS+H K+MANDEHLHKAF FFDQNQSGYIEI+ELR  LN + + T+SE+VI+AIM D
Sbjct: 421 FVAVSVHLKKMANDEHLHKAFNFFDQNQSGYIEIDELREALNDELDNTSSEEVIAAIMQD 480

Query: 481 VDTDKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNL 529
           VDTDKDG ISYEEF AMMKAGTDWRKASRQYSRERFNSLSLKL R+GSL L
Sbjct: 481 VDTDKDGRISYEEFVAMMKAGTDWRKASRQYSRERFNSLSLKLMRDGSLQL 531

BLAST of Sgr023216 vs. ExPASy Swiss-Prot
Match: Q84SL0 (Calcium-dependent protein kinase 20 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK20 PE=2 SV=2)

HSP 1 Score: 803.9 bits (2075), Expect = 1.1e-231
Identity = 404/549 (73.59%), Postives = 461/549 (83.97%), Query Frame = 0

Query: 1   MGNCCATP-----GTPSAKTKVKHK--------------KKANPFSSNDGGNNVNGAGNK 60
           MGNCC TP     G    + + K K              KK NPFS     ++   +G++
Sbjct: 1   MGNCCVTPEGSGRGRKKQQQEQKQKQKEPKQQQQQQKKGKKPNPFSIEYNRSSA-PSGHR 60

Query: 61  LRVLKDPTGHDILAQYDLGRELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIED 120
           L VL++PTG DI A+Y+LG ELGRGEFGVTYLCT+  TG+ +ACKSISKKKLRTA+DIED
Sbjct: 61  LVVLREPTGRDIAARYELGGELGRGEFGVTYLCTERETGDAYACKSISKKKLRTAVDIED 120

Query: 121 VRREVQIMKHLPKHSNIVTLKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAV 180
           VRREV IM+HLPKH NIVTL+DTYEDD AVH+VMELCEGGELFDRIVARGHYTERAAA V
Sbjct: 121 VRREVDIMRHLPKHPNIVTLRDTYEDDNAVHLVMELCEGGELFDRIVARGHYTERAAALV 180

Query: 181 MRTIVEVVQVCHKHGVMHRDLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGS 240
            RTIVEVVQ+CHKHGVMHRDLKPENFLFANKKE++ LKAIDFGLS+ F PGE+F EIVGS
Sbjct: 181 TRTIVEVVQMCHKHGVMHRDLKPENFLFANKKETAALKAIDFGLSVFFTPGERFTEIVGS 240

Query: 241 PYYMAPEVLKRNYGPEVDVWSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPW 300
           PYYMAPEVLKRNYGPEVDVWSAGVILYILLCG+PPFWAETEQGVAQAIIRSVIDF+RDPW
Sbjct: 241 PYYMAPEVLKRNYGPEVDVWSAGVILYILLCGVPPFWAETEQGVAQAIIRSVIDFKRDPW 300

Query: 301 PRVSENAKDLVRKMLNPDPKRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMN 360
           PRVS+NAKDLV+ MLNPDP+RRL A +VLDHPWLQN KKAPNV LGETVKARL+QFSVMN
Sbjct: 301 PRVSDNAKDLVKGMLNPDPRRRLNAQQVLDHPWLQNIKKAPNVNLGETVKARLQQFSVMN 360

Query: 361 KLKKRALRVIAEHLSVEEVAGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQI 420
           K KK ALRVIAEHLSVEEVAGIK+ F+ MD+ K   IN +EL+ G+ KLG Q+ D D+QI
Sbjct: 361 KFKKHALRVIAEHLSVEEVAGIKDMFEKMDLNKDNMINFDELKLGLHKLGHQMADADVQI 420

Query: 421 LMEAAGVHGDGTLNYGEFVAVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNA 480
           LM+AA V G+G+L+YGEFVA+S+H +++ NDEHLHKAFA+FD+NQSGYIEI+ELR  L  
Sbjct: 421 LMDAADVDGNGSLDYGEFVALSVHLRKIGNDEHLHKAFAYFDRNQSGYIEIDELRESLAD 480

Query: 481 DEETNSEDVISAIMHDVDTDKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLF 531
           D   N E+VI+AI+ DVDTDKDG ISY+EFAAMMKAGTDWRKASRQYSRERF SLSLKL 
Sbjct: 481 DLGANHEEVINAIIRDVDTDKDGKISYDEFAAMMKAGTDWRKASRQYSRERFTSLSLKLQ 540

BLAST of Sgr023216 vs. ExPASy Swiss-Prot
Match: Q6NLQ6 (Calcium-dependent protein kinase 32 OS=Arabidopsis thaliana OX=3702 GN=CPK32 PE=1 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 2.7e-230
Identity = 396/534 (74.16%), Postives = 454/534 (85.02%), Query Frame = 0

Query: 1   MGNCCATPGT--PSAKTKVKHKKKANPFS----SNDGGNNVNGAGNKLRVLKDPTGHDIL 60
           MGNCC T G+   +     K +KK NPFS     + GG +  G   KL VL DPTG +I 
Sbjct: 1   MGNCCGTAGSLAQNDNKPKKGRKKQNPFSIDYGLHHGGGDGGGRPLKLIVLNDPTGREIE 60

Query: 61  AQYDLGRELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPK 120
           ++Y LGRELGRGEFGVTYLCTD  T + FACKSI KKKLRTA+DIEDVRREV+IM+H+P+
Sbjct: 61  SKYTLGRELGRGEFGVTYLCTDKETDDVFACKSILKKKLRTAVDIEDVRREVEIMRHMPE 120

Query: 121 HSNIVTLKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHK 180
           H N+VTLK+TYED+ AVH+VMELCEGGELFDRIVARGHYTERAAAAV +TI+EVVQVCHK
Sbjct: 121 HPNVVTLKETYEDEHAVHLVMELCEGGELFDRIVARGHYTERAAAAVTKTIMEVVQVCHK 180

Query: 181 HGVMHRDLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNY 240
           HGVMHRDLKPENFLF NKKE++PLKAIDFGLS+ FKPGE+FNEIVGSPYYMAPEVLKRNY
Sbjct: 181 HGVMHRDLKPENFLFGNKKETAPLKAIDFGLSVFFKPGERFNEIVGSPYYMAPEVLKRNY 240

Query: 241 GPEVDVWSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRK 300
           GPEVD+WSAGVILYILLCG+PPFWAETEQGVAQAIIRSV+DFRRDPWP+VSENAKDL+RK
Sbjct: 241 GPEVDIWSAGVILYILLCGVPPFWAETEQGVAQAIIRSVLDFRRDPWPKVSENAKDLIRK 300

Query: 301 MLNPDPKRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEH 360
           ML+PD KRRLTA +VLDHPWLQNAK APNV LGETV+ARLKQF+VMNKLKKRALRVIAEH
Sbjct: 301 MLDPDQKRRLTAQQVLDHPWLQNAKTAPNVSLGETVRARLKQFTVMNKLKKRALRVIAEH 360

Query: 361 LSVEEVAGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQILMEAAGVHGDGTL 420
           LS EE +GI+E F +MD  +RGKIN++EL+ G+QKLG  IP  DLQILM+A  +  DG L
Sbjct: 361 LSDEEASGIREGFQIMDTSQRGKINIDELKIGLQKLGHAIPQDDLQILMDAGDIDRDGYL 420

Query: 421 NYGEFVAVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAI 480
           +  EF+A+S+H ++M NDEHL KAFAFFDQN +GYIEIEELR  L +DE   SE+V+ AI
Sbjct: 421 DCDEFIAISVHLRKMGNDEHLKKAFAFFDQNNNGYIEIEELREAL-SDELGTSEEVVDAI 480

Query: 481 MHDVDTDKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNL 529
           + DVDTDKDG ISYEEF  MMK GTDWRKASRQYSRERFNS+SLKL ++ SL +
Sbjct: 481 IRDVDTDKDGRISYEEFVTMMKTGTDWRKASRQYSRERFNSISLKLMQDASLQV 533

BLAST of Sgr023216 vs. ExPASy Swiss-Prot
Match: P93759 (Calcium-dependent protein kinase 14 OS=Arabidopsis thaliana OX=3702 GN=CPK14 PE=2 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 2.3e-221
Identity = 378/526 (71.86%), Postives = 448/526 (85.17%), Query Frame = 0

Query: 1   MGNCCATPGTPSAKTKVKHKKKANPFSSNDGGNNVNGAGNKLRVLKDPTGHDILAQYDLG 60
           MGNCC T G+     + K  K  NPF SN+ GN+ +G   KL VLK+PTGH+I  +Y LG
Sbjct: 1   MGNCCGTAGSLIQDKQKKGFKLPNPF-SNEYGNHHDGL--KLIVLKEPTGHEIKQKYKLG 60

Query: 61  RELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHSNIVT 120
           RELGRGEFGVTYLCT+  TGE FACKSI KKKL+T+IDIEDV+REV+IM+ +P+H NIVT
Sbjct: 61  RELGRGEFGVTYLCTEIETGEIFACKSILKKKLKTSIDIEDVKREVEIMRQMPEHPNIVT 120

Query: 121 LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHGVMHR 180
           LK+TYEDD+AVH+VMELCEGGELFDRIVARGHYTERAAA+V++TI+EVVQ+CHKHGVMHR
Sbjct: 121 LKETYEDDKAVHLVMELCEGGELFDRIVARGHYTERAAASVIKTIIEVVQMCHKHGVMHR 180

Query: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEVDV 240
           DLKPENFLFANKKE++ LKAIDFGLS+ FKPGE+FNEIVGSPYYMAPEVL+R+YG E+D+
Sbjct: 181 DLKPENFLFANKKETASLKAIDFGLSVFFKPGERFNEIVGSPYYMAPEVLRRSYGQEIDI 240

Query: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP 300
           WSAGVILYILLCG+PPFWAETE GVA+AI++SVIDF+RDPWP+VS+NAKDL++KML+PDP
Sbjct: 241 WSAGVILYILLCGVPPFWAETEHGVAKAILKSVIDFKRDPWPKVSDNAKDLIKKMLHPDP 300

Query: 301 KRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360
           +RRLTA +VLDHPW+QN K A NV LGETV+ARLKQFSVMNKLKKRALRVIAEHLSVEE 
Sbjct: 301 RRRLTAQQVLDHPWIQNGKNASNVSLGETVRARLKQFSVMNKLKKRALRVIAEHLSVEET 360

Query: 361 AGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQILMEAAGVHGDGTLNYGEFV 420
           + IKE F +MD   RGKI + EL  G+QKLG  +P  D+QILM+A  V  DG L+  EFV
Sbjct: 361 SCIKERFQVMDTSNRGKITITELGIGLQKLGIVVPQDDIQILMDAGDVDKDGYLDVNEFV 420

Query: 421 AVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAIMHDVDT 480
           A+S+H +++ NDEHL KAF FFD+N+SGYIEIEELR  L  D +T SE+V+ AI+ DVDT
Sbjct: 421 AISVHIRKLGNDEHLKKAFTFFDKNKSGYIEIEELRDALADDVDTTSEEVVEAIILDVDT 480

Query: 481 DKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSL 527
           +KDG ISY+EFA MMK GTDWRKASRQYSR+ F  LSLKL ++GSL
Sbjct: 481 NKDGKISYDEFATMMKTGTDWRKASRQYSRDLFKCLSLKLMQDGSL 523

BLAST of Sgr023216 vs. ExPASy TrEMBL
Match: A0A6J1D3X7 (calcium-dependent protein kinase 8-like OS=Momordica charantia OX=3673 GN=LOC111017358 PE=4 SV=1)

HSP 1 Score: 974.2 bits (2517), Expect = 2.2e-280
Identity = 489/534 (91.57%), Postives = 504/534 (94.38%), Query Frame = 0

Query: 1   MGNCCATPGTPSA----KTKVKHKKKANPFSSNDGGNNVNGAGNKLRVLKDPTGHDILAQ 60
           MGNCCATPGTPSA    KTK KHKKK N   ++D    VNGAGNKLRVLK+PTGHDI A 
Sbjct: 1   MGNCCATPGTPSAKPKTKTKGKHKKKGNRSPTDDNRKQVNGAGNKLRVLKEPTGHDISAH 60

Query: 61  YDLGRELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHS 120
           YDLGRELGRGEFGVTYLCTDT+TGEKFACKSISKKKLRTA+DIEDVRREVQIMKHLPKH 
Sbjct: 61  YDLGRELGRGEFGVTYLCTDTNTGEKFACKSISKKKLRTAVDIEDVRREVQIMKHLPKHP 120

Query: 121 NIVTLKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHG 180
           NIVTLKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAA VMRTIVEVVQVCHKHG
Sbjct: 121 NIVTLKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAVVMRTIVEVVQVCHKHG 180

Query: 181 VMHRDLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGP 240
           VMHRDLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYG 
Sbjct: 181 VMHRDLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGQ 240

Query: 241 EVDVWSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKML 300
           EVDVWSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPR+S+NAKDLVRKML
Sbjct: 241 EVDVWSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRISDNAKDLVRKML 300

Query: 301 NPDPKRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLS 360
           NPDPKRRLTA EVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLS
Sbjct: 301 NPDPKRRLTAQEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLS 360

Query: 361 VEEVAGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQILMEAAGVHGDGTLNY 420
           VEEVAGIKEAFD MDVEK+GKI+LEELR G+QKLG QIPD+DLQILMEAAGVH DGTLNY
Sbjct: 361 VEEVAGIKEAFDTMDVEKKGKIHLEELRTGLQKLGHQIPDSDLQILMEAAGVHDDGTLNY 420

Query: 421 GEFVAVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAIMH 480
           GEFVA+SIH KRMANDEHLHKAF+FFDQ+QSGYIEIEELRAVLN +EETNSEDVI AIMH
Sbjct: 421 GEFVAISIHLKRMANDEHLHKAFSFFDQDQSGYIEIEELRAVLNDEEETNSEDVIIAIMH 480

Query: 481 DVDTDKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNLTS 531
           DVDTDKDG ISYEEFA MMKAGTDWRKASRQYSRERFNSLSLKLFREGSL  TS
Sbjct: 481 DVDTDKDGRISYEEFAVMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLKHTS 534

BLAST of Sgr023216 vs. ExPASy TrEMBL
Match: A0A0A0KF18 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G439940 PE=4 SV=1)

HSP 1 Score: 971.1 bits (2509), Expect = 1.9e-279
Identity = 487/530 (91.89%), Postives = 501/530 (94.53%), Query Frame = 0

Query: 1   MGNCCATPGTPSAKTKVKHKKKANPFSSNDGGNNVNGAGNKLRVLKDPTGHDILAQYDLG 60
           MGNCCATP TPSAK K K+ KKANPF + DG  N NG GNKL+VL DPTGH+I A YDLG
Sbjct: 1   MGNCCATPSTPSAKPKGKN-KKANPFPAKDGEKNANGHGNKLQVLSDPTGHNISAHYDLG 60

Query: 61  RELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHSNIVT 120
           RELGRGEFGVTYLCTDT+TGEKFACKSISKKKLRTA+DIEDVRREVQIMKHLP H N+V 
Sbjct: 61  RELGRGEFGVTYLCTDTNTGEKFACKSISKKKLRTAVDIEDVRREVQIMKHLPNHPNVVR 120

Query: 121 LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHGVMHR 180
           LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAA VMRTIVEVVQ CHKHGVMHR
Sbjct: 121 LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAVVMRTIVEVVQACHKHGVMHR 180

Query: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEVDV 240
           DLKPENFLFANKKESSPLKAIDFGLSITFKPG+ FNEIVGSPYYMAPEVLKRNYGPEVDV
Sbjct: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKPGDLFNEIVGSPYYMAPEVLKRNYGPEVDV 240

Query: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP 300
           WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP
Sbjct: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP 300

Query: 301 KRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360
           KRRLTA EVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV
Sbjct: 301 KRRLTAQEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360

Query: 361 AGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQILMEAAGVHGDGTLNYGEFV 420
           AGIKEAFDMMDVEKRGKINLEELR+G+QKLGQQIP+ DLQILMEAAGV  DG LNYGEFV
Sbjct: 361 AGIKEAFDMMDVEKRGKINLEELRSGLQKLGQQIPEADLQILMEAAGVQDDGALNYGEFV 420

Query: 421 AVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAIMHDVDT 480
           AVSIH KRMANDEHLHKAFAFFDQNQSG+IEIEELRAVL  DEETNSEDV++AIMHDVDT
Sbjct: 421 AVSIHLKRMANDEHLHKAFAFFDQNQSGFIEIEELRAVLLNDEETNSEDVVNAIMHDVDT 480

Query: 481 DKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNLTS 531
           DKDG ISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSL LTS
Sbjct: 481 DKDGRISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLKLTS 529

BLAST of Sgr023216 vs. ExPASy TrEMBL
Match: A0A6J1FYR5 (calcium-dependent protein kinase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111448998 PE=4 SV=1)

HSP 1 Score: 969.9 bits (2506), Expect = 4.2e-279
Identity = 484/530 (91.32%), Postives = 504/530 (95.09%), Query Frame = 0

Query: 1   MGNCCATPGTPSAKTKVKHKKKANPFSSNDGGNNVNGAGNKLRVLKDPTGHDILAQYDLG 60
           MGNCCATP +PS K K KHKKK+ PFS     +NV GA NKLRVLK PTGHDI A YDLG
Sbjct: 1   MGNCCATPVSPSGKPKGKHKKKSFPFSGKGSRSNVVGADNKLRVLKAPTGHDISAHYDLG 60

Query: 61  RELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHSNIVT 120
           RELGRGEFGVTYLCTDT+TGE FACKSISKKKLRTA+DIEDVRREVQIMKHLPKH NIVT
Sbjct: 61  RELGRGEFGVTYLCTDTNTGESFACKSISKKKLRTAVDIEDVRREVQIMKHLPKHPNIVT 120

Query: 121 LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHGVMHR 180
           LKDTYEDD+AVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVE+V+ CHKHGVMHR
Sbjct: 121 LKDTYEDDEAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEIVEACHKHGVMHR 180

Query: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEVDV 240
           DLKPENFLFANKKESSPLKAIDFGLSITFK GEQFNEIVGSPYYMAPEVLK+NYGPEVDV
Sbjct: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKRGEQFNEIVGSPYYMAPEVLKKNYGPEVDV 240

Query: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP 300
           WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKML+PDP
Sbjct: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLDPDP 300

Query: 301 KRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360
           KRRLTA EVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV
Sbjct: 301 KRRLTAQEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360

Query: 361 AGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQILMEAAGVHGDGTLNYGEFV 420
           AGIK+AF+MMDVEKRGKINLEELR+G+QKLGQQIP+ DLQILMEAAGVH DGTLNYGEFV
Sbjct: 361 AGIKDAFEMMDVEKRGKINLEELRSGLQKLGQQIPEADLQILMEAAGVHDDGTLNYGEFV 420

Query: 421 AVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAIMHDVDT 480
           A+SIH +RMANDEHLHKAFAFFDQNQSG+IEIEELRAVLN DEETNSEDVISAIMHDVDT
Sbjct: 421 ALSIHLRRMANDEHLHKAFAFFDQNQSGFIEIEELRAVLN-DEETNSEDVISAIMHDVDT 480

Query: 481 DKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNLTS 531
           DKDGCISYEEFA MMKAGTDWRKASRQYSRERFN+LSLKLFREGS NLT+
Sbjct: 481 DKDGCISYEEFAGMMKAGTDWRKASRQYSRERFNNLSLKLFREGSFNLTN 529

BLAST of Sgr023216 vs. ExPASy TrEMBL
Match: A0A1S4DXT0 (calcium-dependent protein kinase 8-like OS=Cucumis melo OX=3656 GN=LOC103491504 PE=4 SV=1)

HSP 1 Score: 968.0 bits (2501), Expect = 1.6e-278
Identity = 486/530 (91.70%), Postives = 501/530 (94.53%), Query Frame = 0

Query: 1   MGNCCATPGTPSAKTKVKHKKKANPFSSNDGGNNVNGAGNKLRVLKDPTGHDILAQYDLG 60
           MGNCCATP TPSAK K K+ KK NPFS+ D   NVNG GNKL+VL DPTGH+I A YDLG
Sbjct: 1   MGNCCATPSTPSAKPKGKN-KKTNPFSAKDVEKNVNGHGNKLQVLSDPTGHNISAHYDLG 60

Query: 61  RELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHSNIVT 120
           RELGRGEFGVTYLCTDT+TGEKFACKSISKKKLRT++DIEDVRREVQIMKHLP H N+V 
Sbjct: 61  RELGRGEFGVTYLCTDTNTGEKFACKSISKKKLRTSVDIEDVRREVQIMKHLPNHPNVVM 120

Query: 121 LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHGVMHR 180
           LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAA VMRTIVEVVQ CHKHGVMHR
Sbjct: 121 LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAVVMRTIVEVVQACHKHGVMHR 180

Query: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEVDV 240
           DLKPENFLFANKKESSPLKAIDFGLSITFKPG+ FNEIVGSPYYMAPEVLKRNYGPEVDV
Sbjct: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKPGDVFNEIVGSPYYMAPEVLKRNYGPEVDV 240

Query: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP 300
           WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP
Sbjct: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP 300

Query: 301 KRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360
           KRRLTA EVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV
Sbjct: 301 KRRLTAQEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360

Query: 361 AGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQILMEAAGVHGDGTLNYGEFV 420
           AGIKEAFDMMDVEKRGKINLEELR+G+QKLGQQIP+ DLQIL+EAAGV  DG LNYGEFV
Sbjct: 361 AGIKEAFDMMDVEKRGKINLEELRSGLQKLGQQIPEADLQILIEAAGVQDDGALNYGEFV 420

Query: 421 AVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAIMHDVDT 480
           AVSIH KRMANDEHLHKAFAFFDQNQSG+IEIEELRAVL  DEETNSEDV+SAIMHDVDT
Sbjct: 421 AVSIHLKRMANDEHLHKAFAFFDQNQSGFIEIEELRAVLLDDEETNSEDVVSAIMHDVDT 480

Query: 481 DKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNLTS 531
           DKDG ISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSL LTS
Sbjct: 481 DKDGRISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLKLTS 529

BLAST of Sgr023216 vs. ExPASy TrEMBL
Match: A0A5D3BA14 (Calcium-dependent protein kinase 8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00990 PE=4 SV=1)

HSP 1 Score: 968.0 bits (2501), Expect = 1.6e-278
Identity = 486/530 (91.70%), Postives = 501/530 (94.53%), Query Frame = 0

Query: 1   MGNCCATPGTPSAKTKVKHKKKANPFSSNDGGNNVNGAGNKLRVLKDPTGHDILAQYDLG 60
           MGNCCATP TPSAK K K+ KK NPFS+ D   NVNG GNKL+VL DPTGH+I A YDLG
Sbjct: 1   MGNCCATPSTPSAKPKGKN-KKTNPFSAKDVEKNVNGHGNKLQVLSDPTGHNISAHYDLG 60

Query: 61  RELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHSNIVT 120
           RELGRGEFGVTYLCTDT+TGEKFACKSISKKKLRT++DIEDVRREVQIMKHLP H N+V 
Sbjct: 61  RELGRGEFGVTYLCTDTNTGEKFACKSISKKKLRTSVDIEDVRREVQIMKHLPNHPNVVM 120

Query: 121 LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHGVMHR 180
           LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAA VMRTIVEVVQ CHKHGVMHR
Sbjct: 121 LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAVVMRTIVEVVQACHKHGVMHR 180

Query: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEVDV 240
           DLKPENFLFANKKESSPLKAIDFGLSITFKPG+ FNEIVGSPYYMAPEVLKRNYGPEVDV
Sbjct: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKPGDVFNEIVGSPYYMAPEVLKRNYGPEVDV 240

Query: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP 300
           WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP
Sbjct: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP 300

Query: 301 KRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360
           KRRLTA EVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV
Sbjct: 301 KRRLTAQEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360

Query: 361 AGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQILMEAAGVHGDGTLNYGEFV 420
           AGIKEAFDMMDVEKRGKINLEELR+G+QKLGQQIP+ DLQIL+EAAGV  DG LNYGEFV
Sbjct: 361 AGIKEAFDMMDVEKRGKINLEELRSGLQKLGQQIPEADLQILIEAAGVQDDGALNYGEFV 420

Query: 421 AVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAIMHDVDT 480
           AVSIH KRMANDEHLHKAFAFFDQNQSG+IEIEELRAVL  DEETNSEDV+SAIMHDVDT
Sbjct: 421 AVSIHLKRMANDEHLHKAFAFFDQNQSGFIEIEELRAVLLDDEETNSEDVVSAIMHDVDT 480

Query: 481 DKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNLTS 531
           DKDG ISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSL LTS
Sbjct: 481 DKDGRISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLKLTS 529

BLAST of Sgr023216 vs. TAIR 10
Match: AT5G19450.1 (calcium-dependent protein kinase 19 )

HSP 1 Score: 885.6 bits (2287), Expect = 2.0e-257
Identity = 439/529 (82.99%), Postives = 479/529 (90.55%), Query Frame = 0

Query: 1   MGNCCATPGTPSAKTKVKHKKKANPFSSNDGGNNVNGAGNKLRVLKDPTGHDILAQYDLG 60
           MGNCCA+PG+ +   K K K K+NPF S     N +G G KL VLKDPTGHDI   YDLG
Sbjct: 1   MGNCCASPGSETGSKKGKPKIKSNPFYSEAYTTNGSGTGFKLSVLKDPTGHDISLMYDLG 60

Query: 61  RELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHSNIVT 120
           RE+GRGEFG+TYLCTD  TGEK+ACKSISKKKLRTA+DIEDVRREV+IMKH+P+H NIV+
Sbjct: 61  REVGRGEFGITYLCTDIKTGEKYACKSISKKKLRTAVDIEDVRREVEIMKHMPRHPNIVS 120

Query: 121 LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHGVMHR 180
           LKD +EDD AVHIVMELCEGGELFDRIVARGHYTERAAAAVM+TI+EVVQ+CHKHGVMHR
Sbjct: 121 LKDAFEDDDAVHIVMELCEGGELFDRIVARGHYTERAAAAVMKTILEVVQICHKHGVMHR 180

Query: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEVDV 240
           DLKPENFLFANKKE+S LKAIDFGLS+ FKPGE FNEIVGSPYYMAPEVL+RNYGPEVD+
Sbjct: 181 DLKPENFLFANKKETSALKAIDFGLSVFFKPGEGFNEIVGSPYYMAPEVLRRNYGPEVDI 240

Query: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP 300
           WSAGVILYILLCG+PPFWAETEQGVAQAIIRSVIDF+RDPWPRVSE AKDLVRKML PDP
Sbjct: 241 WSAGVILYILLCGVPPFWAETEQGVAQAIIRSVIDFKRDPWPRVSETAKDLVRKMLEPDP 300

Query: 301 KRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360
           K+RL+A +VL+H W+QNAKKAPNV LGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV
Sbjct: 301 KKRLSAAQVLEHSWIQNAKKAPNVSLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360

Query: 361 AGIKEAFDMMDVEKRGKINLEELRNGMQKLG-QQIPDTDLQILMEAAGVHGDGTLNYGEF 420
           AGIKEAF+MMD +K GKINLEEL+ G+ KLG QQIPDTDLQILMEAA V GDGTLNYGEF
Sbjct: 361 AGIKEAFEMMDSKKTGKINLEELKFGLHKLGQQQIPDTDLQILMEAADVDGDGTLNYGEF 420

Query: 421 VAVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAIMHDVD 480
           VAVS+H K+MANDEHLHKAF+FFDQNQS YIEIEELR  LN + +TNSE+V++AIM DVD
Sbjct: 421 VAVSVHLKKMANDEHLHKAFSFFDQNQSDYIEIEELREALNDEVDTNSEEVVAAIMQDVD 480

Query: 481 TDKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNL 529
           TDKDG ISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKL REGSL L
Sbjct: 481 TDKDGRISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLMREGSLQL 529

BLAST of Sgr023216 vs. TAIR 10
Match: AT5G19450.2 (calcium-dependent protein kinase 19 )

HSP 1 Score: 885.6 bits (2287), Expect = 2.0e-257
Identity = 439/529 (82.99%), Postives = 479/529 (90.55%), Query Frame = 0

Query: 1   MGNCCATPGTPSAKTKVKHKKKANPFSSNDGGNNVNGAGNKLRVLKDPTGHDILAQYDLG 60
           MGNCCA+PG+ +   K K K K+NPF S     N +G G KL VLKDPTGHDI   YDLG
Sbjct: 1   MGNCCASPGSETGSKKGKPKIKSNPFYSEAYTTNGSGTGFKLSVLKDPTGHDISLMYDLG 60

Query: 61  RELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHSNIVT 120
           RE+GRGEFG+TYLCTD  TGEK+ACKSISKKKLRTA+DIEDVRREV+IMKH+P+H NIV+
Sbjct: 61  REVGRGEFGITYLCTDIKTGEKYACKSISKKKLRTAVDIEDVRREVEIMKHMPRHPNIVS 120

Query: 121 LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHGVMHR 180
           LKD +EDD AVHIVMELCEGGELFDRIVARGHYTERAAAAVM+TI+EVVQ+CHKHGVMHR
Sbjct: 121 LKDAFEDDDAVHIVMELCEGGELFDRIVARGHYTERAAAAVMKTILEVVQICHKHGVMHR 180

Query: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEVDV 240
           DLKPENFLFANKKE+S LKAIDFGLS+ FKPGE FNEIVGSPYYMAPEVL+RNYGPEVD+
Sbjct: 181 DLKPENFLFANKKETSALKAIDFGLSVFFKPGEGFNEIVGSPYYMAPEVLRRNYGPEVDI 240

Query: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP 300
           WSAGVILYILLCG+PPFWAETEQGVAQAIIRSVIDF+RDPWPRVSE AKDLVRKML PDP
Sbjct: 241 WSAGVILYILLCGVPPFWAETEQGVAQAIIRSVIDFKRDPWPRVSETAKDLVRKMLEPDP 300

Query: 301 KRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360
           K+RL+A +VL+H W+QNAKKAPNV LGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV
Sbjct: 301 KKRLSAAQVLEHSWIQNAKKAPNVSLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360

Query: 361 AGIKEAFDMMDVEKRGKINLEELRNGMQKLG-QQIPDTDLQILMEAAGVHGDGTLNYGEF 420
           AGIKEAF+MMD +K GKINLEEL+ G+ KLG QQIPDTDLQILMEAA V GDGTLNYGEF
Sbjct: 361 AGIKEAFEMMDSKKTGKINLEELKFGLHKLGQQQIPDTDLQILMEAADVDGDGTLNYGEF 420

Query: 421 VAVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAIMHDVD 480
           VAVS+H K+MANDEHLHKAF+FFDQNQS YIEIEELR  LN + +TNSE+V++AIM DVD
Sbjct: 421 VAVSVHLKKMANDEHLHKAFSFFDQNQSDYIEIEELREALNDEVDTNSEEVVAAIMQDVD 480

Query: 481 TDKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNL 529
           TDKDG ISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKL REGSL L
Sbjct: 481 TDKDGRISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLMREGSLQL 529

BLAST of Sgr023216 vs. TAIR 10
Match: AT5G12480.1 (calmodulin-domain protein kinase 7 )

HSP 1 Score: 872.5 bits (2253), Expect = 1.8e-253
Identity = 435/531 (81.92%), Postives = 478/531 (90.02%), Query Frame = 0

Query: 1   MGNCCATPGTPSAKTKV-KHKKKANPFSSND-GGNNVNGAGNKLRVLKDPTGHDILAQYD 60
           MGNCC  P + + ++K  K K K NPF SN+    + +GAG KL VLKDPTGHDI  QYD
Sbjct: 1   MGNCCGNPSSATNQSKQGKPKNKNNPFYSNEYATTDRSGAGFKLSVLKDPTGHDISLQYD 60

Query: 61  LGRELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHSNI 120
           LGRE+GRGEFG+TYLCTD  TGEK+ACKSISKKKLRTA+DIEDVRREV+IMKH+PKH N+
Sbjct: 61  LGREVGRGEFGITYLCTDKETGEKYACKSISKKKLRTAVDIEDVRREVEIMKHMPKHPNV 120

Query: 121 VTLKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHGVM 180
           V+LKD++EDD AVHIVMELCEGGELFDRIVARGHYTERAAAAVM+TIVEVVQ+CHK GVM
Sbjct: 121 VSLKDSFEDDDAVHIVMELCEGGELFDRIVARGHYTERAAAAVMKTIVEVVQICHKQGVM 180

Query: 181 HRDLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEV 240
           HRDLKPENFLFANKKE+S LKAIDFGLS+ FKPGEQFNEIVGSPYYMAPEVL+RNYGPE+
Sbjct: 181 HRDLKPENFLFANKKETSALKAIDFGLSVFFKPGEQFNEIVGSPYYMAPEVLRRNYGPEI 240

Query: 241 DVWSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNP 300
           DVWSAGVILYILLCG+PPFWAETEQGVAQAIIRSVIDF+RDPWPRVS++AKDLVRKML P
Sbjct: 241 DVWSAGVILYILLCGVPPFWAETEQGVAQAIIRSVIDFKRDPWPRVSDSAKDLVRKMLEP 300

Query: 301 DPKRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVE 360
           DPK+RLTA +VL+H W+ NAKKAPNV LGETVKARLKQFSVMNKLKKRALRVIAEHLSVE
Sbjct: 301 DPKKRLTAAQVLEHTWILNAKKAPNVSLGETVKARLKQFSVMNKLKKRALRVIAEHLSVE 360

Query: 361 EVAGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQILMEAAGVHGDGTLNYGE 420
           E AGIKEAF+MMDV KRGKINLEEL+ G+QK GQQI DTDLQILMEA  V GDGTLNY E
Sbjct: 361 EAAGIKEAFEMMDVNKRGKINLEELKYGLQKAGQQIADTDLQILMEATDVDGDGTLNYSE 420

Query: 421 FVAVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNAD-EETNSEDVISAIMHD 480
           FVAVS+H K+MANDEHLHKAF FFDQNQSGYIEI+ELR  LN + + T+SE+VI+AIM D
Sbjct: 421 FVAVSVHLKKMANDEHLHKAFNFFDQNQSGYIEIDELREALNDELDNTSSEEVIAAIMQD 480

Query: 481 VDTDKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNL 529
           VDTDKDG ISYEEF AMMKAGTDWRKASRQYSRERFNSLSLKL R+GSL L
Sbjct: 481 VDTDKDGRISYEEFVAMMKAGTDWRKASRQYSRERFNSLSLKLMRDGSLQL 531

BLAST of Sgr023216 vs. TAIR 10
Match: AT3G57530.1 (calcium-dependent protein kinase 32 )

HSP 1 Score: 799.3 bits (2063), Expect = 1.9e-231
Identity = 396/534 (74.16%), Postives = 454/534 (85.02%), Query Frame = 0

Query: 1   MGNCCATPGT--PSAKTKVKHKKKANPFS----SNDGGNNVNGAGNKLRVLKDPTGHDIL 60
           MGNCC T G+   +     K +KK NPFS     + GG +  G   KL VL DPTG +I 
Sbjct: 1   MGNCCGTAGSLAQNDNKPKKGRKKQNPFSIDYGLHHGGGDGGGRPLKLIVLNDPTGREIE 60

Query: 61  AQYDLGRELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPK 120
           ++Y LGRELGRGEFGVTYLCTD  T + FACKSI KKKLRTA+DIEDVRREV+IM+H+P+
Sbjct: 61  SKYTLGRELGRGEFGVTYLCTDKETDDVFACKSILKKKLRTAVDIEDVRREVEIMRHMPE 120

Query: 121 HSNIVTLKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHK 180
           H N+VTLK+TYED+ AVH+VMELCEGGELFDRIVARGHYTERAAAAV +TI+EVVQVCHK
Sbjct: 121 HPNVVTLKETYEDEHAVHLVMELCEGGELFDRIVARGHYTERAAAAVTKTIMEVVQVCHK 180

Query: 181 HGVMHRDLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNY 240
           HGVMHRDLKPENFLF NKKE++PLKAIDFGLS+ FKPGE+FNEIVGSPYYMAPEVLKRNY
Sbjct: 181 HGVMHRDLKPENFLFGNKKETAPLKAIDFGLSVFFKPGERFNEIVGSPYYMAPEVLKRNY 240

Query: 241 GPEVDVWSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRK 300
           GPEVD+WSAGVILYILLCG+PPFWAETEQGVAQAIIRSV+DFRRDPWP+VSENAKDL+RK
Sbjct: 241 GPEVDIWSAGVILYILLCGVPPFWAETEQGVAQAIIRSVLDFRRDPWPKVSENAKDLIRK 300

Query: 301 MLNPDPKRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEH 360
           ML+PD KRRLTA +VLDHPWLQNAK APNV LGETV+ARLKQF+VMNKLKKRALRVIAEH
Sbjct: 301 MLDPDQKRRLTAQQVLDHPWLQNAKTAPNVSLGETVRARLKQFTVMNKLKKRALRVIAEH 360

Query: 361 LSVEEVAGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQILMEAAGVHGDGTL 420
           LS EE +GI+E F +MD  +RGKIN++EL+ G+QKLG  IP  DLQILM+A  +  DG L
Sbjct: 361 LSDEEASGIREGFQIMDTSQRGKINIDELKIGLQKLGHAIPQDDLQILMDAGDIDRDGYL 420

Query: 421 NYGEFVAVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAI 480
           +  EF+A+S+H ++M NDEHL KAFAFFDQN +GYIEIEELR  L +DE   SE+V+ AI
Sbjct: 421 DCDEFIAISVHLRKMGNDEHLKKAFAFFDQNNNGYIEIEELREAL-SDELGTSEEVVDAI 480

Query: 481 MHDVDTDKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSLNL 529
           + DVDTDKDG ISYEEF  MMK GTDWRKASRQYSRERFNS+SLKL ++ SL +
Sbjct: 481 IRDVDTDKDGRISYEEFVTMMKTGTDWRKASRQYSRERFNSISLKLMQDASLQV 533

BLAST of Sgr023216 vs. TAIR 10
Match: AT2G41860.1 (calcium-dependent protein kinase 14 )

HSP 1 Score: 769.6 bits (1986), Expect = 1.6e-222
Identity = 378/526 (71.86%), Postives = 448/526 (85.17%), Query Frame = 0

Query: 1   MGNCCATPGTPSAKTKVKHKKKANPFSSNDGGNNVNGAGNKLRVLKDPTGHDILAQYDLG 60
           MGNCC T G+     + K  K  NPF SN+ GN+ +G   KL VLK+PTGH+I  +Y LG
Sbjct: 1   MGNCCGTAGSLIQDKQKKGFKLPNPF-SNEYGNHHDGL--KLIVLKEPTGHEIKQKYKLG 60

Query: 61  RELGRGEFGVTYLCTDTSTGEKFACKSISKKKLRTAIDIEDVRREVQIMKHLPKHSNIVT 120
           RELGRGEFGVTYLCT+  TGE FACKSI KKKL+T+IDIEDV+REV+IM+ +P+H NIVT
Sbjct: 61  RELGRGEFGVTYLCTEIETGEIFACKSILKKKLKTSIDIEDVKREVEIMRQMPEHPNIVT 120

Query: 121 LKDTYEDDQAVHIVMELCEGGELFDRIVARGHYTERAAAAVMRTIVEVVQVCHKHGVMHR 180
           LK+TYEDD+AVH+VMELCEGGELFDRIVARGHYTERAAA+V++TI+EVVQ+CHKHGVMHR
Sbjct: 121 LKETYEDDKAVHLVMELCEGGELFDRIVARGHYTERAAASVIKTIIEVVQMCHKHGVMHR 180

Query: 181 DLKPENFLFANKKESSPLKAIDFGLSITFKPGEQFNEIVGSPYYMAPEVLKRNYGPEVDV 240
           DLKPENFLFANKKE++ LKAIDFGLS+ FKPGE+FNEIVGSPYYMAPEVL+R+YG E+D+
Sbjct: 181 DLKPENFLFANKKETASLKAIDFGLSVFFKPGERFNEIVGSPYYMAPEVLRRSYGQEIDI 240

Query: 241 WSAGVILYILLCGIPPFWAETEQGVAQAIIRSVIDFRRDPWPRVSENAKDLVRKMLNPDP 300
           WSAGVILYILLCG+PPFWAETE GVA+AI++SVIDF+RDPWP+VS+NAKDL++KML+PDP
Sbjct: 241 WSAGVILYILLCGVPPFWAETEHGVAKAILKSVIDFKRDPWPKVSDNAKDLIKKMLHPDP 300

Query: 301 KRRLTALEVLDHPWLQNAKKAPNVPLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEV 360
           +RRLTA +VLDHPW+QN K A NV LGETV+ARLKQFSVMNKLKKRALRVIAEHLSVEE 
Sbjct: 301 RRRLTAQQVLDHPWIQNGKNASNVSLGETVRARLKQFSVMNKLKKRALRVIAEHLSVEET 360

Query: 361 AGIKEAFDMMDVEKRGKINLEELRNGMQKLGQQIPDTDLQILMEAAGVHGDGTLNYGEFV 420
           + IKE F +MD   RGKI + EL  G+QKLG  +P  D+QILM+A  V  DG L+  EFV
Sbjct: 361 SCIKERFQVMDTSNRGKITITELGIGLQKLGIVVPQDDIQILMDAGDVDKDGYLDVNEFV 420

Query: 421 AVSIHFKRMANDEHLHKAFAFFDQNQSGYIEIEELRAVLNADEETNSEDVISAIMHDVDT 480
           A+S+H +++ NDEHL KAF FFD+N+SGYIEIEELR  L  D +T SE+V+ AI+ DVDT
Sbjct: 421 AISVHIRKLGNDEHLKKAFTFFDKNKSGYIEIEELRDALADDVDTTSEEVVEAIILDVDT 480

Query: 481 DKDGCISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLFREGSL 527
           +KDG ISY+EFA MMK GTDWRKASRQYSR+ F  LSLKL ++GSL
Sbjct: 481 NKDGKISYDEFATMMKTGTDWRKASRQYSRDLFKCLSLKLMQDGSL 523

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038901584.16.7e-28794.35calcium-dependent protein kinase 8-like [Benincasa hispida] >XP_038901585.1 calc... [more]
XP_022148790.14.6e-28091.57calcium-dependent protein kinase 8-like [Momordica charantia][more]
XP_011657639.13.9e-27991.89calcium-dependent protein kinase 8 [Cucumis sativus] >KGN48128.1 hypothetical pr... [more]
XP_022944585.18.7e-27991.32calcium-dependent protein kinase 8-like [Cucurbita moschata][more]
KAG7010274.12.5e-27891.13Calcium-dependent protein kinase 8 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q424382.8e-25682.99Calcium-dependent protein kinase 8 OS=Arabidopsis thaliana OX=3702 GN=CPK8 PE=1 ... [more]
Q388732.5e-25281.92Calcium-dependent protein kinase 7 OS=Arabidopsis thaliana OX=3702 GN=CPK7 PE=2 ... [more]
Q84SL01.1e-23173.59Calcium-dependent protein kinase 20 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q6NLQ62.7e-23074.16Calcium-dependent protein kinase 32 OS=Arabidopsis thaliana OX=3702 GN=CPK32 PE=... [more]
P937592.3e-22171.86Calcium-dependent protein kinase 14 OS=Arabidopsis thaliana OX=3702 GN=CPK14 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1D3X72.2e-28091.57calcium-dependent protein kinase 8-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A0A0KF181.9e-27991.89Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G439940 PE=4 SV=1[more]
A0A6J1FYR54.2e-27991.32calcium-dependent protein kinase 8-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A1S4DXT01.6e-27891.70calcium-dependent protein kinase 8-like OS=Cucumis melo OX=3656 GN=LOC103491504 ... [more]
A0A5D3BA141.6e-27891.70Calcium-dependent protein kinase 8-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
Match NameE-valueIdentityDescription
AT5G19450.12.0e-25782.99calcium-dependent protein kinase 19 [more]
AT5G19450.22.0e-25782.99calcium-dependent protein kinase 19 [more]
AT5G12480.11.8e-25381.92calmodulin-domain protein kinase 7 [more]
AT3G57530.11.9e-23174.16calcium-dependent protein kinase 32 [more]
AT2G41860.11.6e-22271.86calcium-dependent protein kinase 14 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 398..426
e-value: 120.0
score: 0.9
coord: 362..390
e-value: 3.0E-4
score: 30.1
coord: 470..498
e-value: 9.3E-5
score: 31.8
coord: 434..462
e-value: 0.0024
score: 27.1
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 363..421
e-value: 2.2E-7
score: 31.2
coord: 433..496
e-value: 1.7E-15
score: 57.2
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 358..393
score: 13.202322
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 466..501
score: 13.704439
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 430..465
score: 12.449146
IPR002048EF-hand domainCDDcd00051EFhcoord: 434..496
e-value: 2.37193E-18
score: 77.2029
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 57..315
e-value: 3.8E-103
score: 358.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 57..315
e-value: 5.7E-78
score: 261.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 57..315
score: 49.587032
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 59..142
e-value: 2.4E-29
score: 103.5
NoneNo IPR availableGENE3D1.10.238.10coord: 324..498
e-value: 5.7E-50
score: 171.4
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 143..323
e-value: 4.3E-60
score: 204.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..38
NoneNo IPR availablePANTHERPTHR24349:SF444CALCIUM-DEPENDENT PROTEIN KINASE 8coord: 13..512
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 13..512
NoneNo IPR availableCDDcd05117STKc_CAMKcoord: 56..314
e-value: 1.37029E-142
score: 409.173
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 177..189
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 443..455
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 479..491
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 63..86
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 54..325
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 344..520

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr023216.1Sgr023216.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0009931 calcium-dependent protein serine/threonine kinase activity
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0004672 protein kinase activity