Sgr022086 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr022086
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	subtilisin-like protease SBT5.3
Location	tig00153874: 965533 .. 966054 (+)
RNA-Seq Expression	Sgr022086
Synteny	Sgr022086

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCATTCGATTTTGGACTTCACCGGAGGCAAAGCAACCCCATTAGAGTACGGTGCAGGACATGTCCATCCAAACAATGCAATGGACCCCGGTCTTGTTTATGACGCTACCGTCGACGACTACCTGAACTTCTTATGTGCACGAGGCTACAACCAGACACAAATCAAGCAATTCTCTAACAAGCCATTCACTTGTGCCAAATCATTCGCACTCACAGATCTCAACTACCCATCGATCTCGGTCCCAAATCTGCAAATGGGTGCCACCGTGACAATCAATAGAAGAGTTAAGAATGTGGGAAGTGTAGGCACGTATGTCGCACGGGTCAAGATGCCCTTGGGAGTTGTGGTTTCGGTCGAGCCGAGTACGTTGCAGTTTAGCAGTGTGGGTGAAGAGAAGGCTTTCAAGGTCGTATTGCAACATACAGGAAAAGTGCAACGGGGGGGCTATGTGTTTGGGACATTGGTGTGGTCAGATGGCAAGCATTTTGTTAGAAGTCCTATTGCAGTGAAATTGGGATGA

mRNA sequence

ATGCATTCGATTTTGGACTTCACCGGAGGCAAAGCAACCCCATTAGAGTACGGTGCAGGACATGTCCATCCAAACAATGCAATGGACCCCGGTCTTGTTTATGACGCTACCGTCGACGACTACCTGAACTTCTTATGTGCACGAGGCTACAACCAGACACAAATCAAGCAATTCTCTAACAAGCCATTCACTTGTGCCAAATCATTCGCACTCACAGATCTCAACTACCCATCGATCTCGGTCCCAAATCTGCAAATGGGTGCCACCGTGACAATCAATAGAAGAGTTAAGAATGTGGGAAGTGTAGGCACGTATGTCGCACGGGTCAAGATGCCCTTGGGAGTTGTGGTTTCGGTCGAGCCGAGTACGTTGCAGTTTAGCAGTGTGGGTGAAGAGAAGGCTTTCAAGGTCGTATTGCAACATACAGGAAAAGTGCAACGGGGGGGCTATGTGTTTGGGACATTGGTGTGGTCAGATGGCAAGCATTTTGTTAGAAGTCCTATTGCAGTGAAATTGGGATGA

Coding sequence (CDS)

ATGCATTCGATTTTGGACTTCACCGGAGGCAAAGCAACCCCATTAGAGTACGGTGCAGGACATGTCCATCCAAACAATGCAATGGACCCCGGTCTTGTTTATGACGCTACCGTCGACGACTACCTGAACTTCTTATGTGCACGAGGCTACAACCAGACACAAATCAAGCAATTCTCTAACAAGCCATTCACTTGTGCCAAATCATTCGCACTCACAGATCTCAACTACCCATCGATCTCGGTCCCAAATCTGCAAATGGGTGCCACCGTGACAATCAATAGAAGAGTTAAGAATGTGGGAAGTGTAGGCACGTATGTCGCACGGGTCAAGATGCCCTTGGGAGTTGTGGTTTCGGTCGAGCCGAGTACGTTGCAGTTTAGCAGTGTGGGTGAAGAGAAGGCTTTCAAGGTCGTATTGCAACATACAGGAAAAGTGCAACGGGGGGGCTATGTGTTTGGGACATTGGTGTGGTCAGATGGCAAGCATTTTGTTAGAAGTCCTATTGCAGTGAAATTGGGATGA

Protein sequence

MHSILDFTGGKATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNKPFTCAKSFALTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARVKMPLGVVVSVEPSTLQFSSVGEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVKLG

Homology

[Close]

BLAST of Sgr022086 vs. NCBI nr
Match: XP_022143462.1 (subtilisin-like protease SBT5.4 [Momordica charantia])

HSP 1 Score: 289.7 bits (740), Expect = 1.7e-74
Identity = 138/171 (80.70%), Postives = 153/171 (89.47%), Query Frame = 0


Query: 3   SILDFTGGKATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNKP 62
           SILDFT GKA+PLEYGAGHV PN+AMDPGLVYD T+DDYLN+LCARG NQTQ++QFSNKP
Sbjct: 596 SILDFTKGKASPLEYGAGHVQPNDAMDPGLVYDTTIDDYLNYLCARGANQTQLRQFSNKP 655

Query: 63  FTCAKSFALTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARVKMPLGVVVSVEPS 122
           FTCAKSFALTDLNYPSI+VPNL++GA  T+NRRVKNVGS GTY ARVK PLGV V VEPS
Sbjct: 656 FTCAKSFALTDLNYPSIAVPNLKVGAPTTVNRRVKNVGSPGTYTARVKAPLGVSVVVEPS 715

Query: 123 TLQFSSVGEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVKLG 174
           TLQF + GEEKAFKVVLQ T K ++G Y+FGT+VWSDGKHFVRSPIAVK G
Sbjct: 716 TLQFRTAGEEKAFKVVLQTTPKAEQGVYLFGTMVWSDGKHFVRSPIAVKSG 766

[Close]

BLAST of Sgr022086 vs. NCBI nr
Match: XP_038904202.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])

HSP 1 Score: 275.8 bits (704), Expect = 2.6e-70
Identity = 128/173 (73.99%), Postives = 149/173 (86.13%), Query Frame = 0


Query: 1   MHSILDFTGGKATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSN 60
           MH ILDFT  +ATP +YGAG V+PN+A+DPGLVYD T+DD LNFLCARGYN TQ+K+FSN
Sbjct: 118 MHPILDFTNVEATPFDYGAGQVNPNDAIDPGLVYDTTIDDCLNFLCARGYNNTQLKKFSN 177

Query: 61  KPFTCAKSFALTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARVKMPLGVVVSVE 120
           KPF CAKSFA+ DLNYPSIS+P L +   VTINRRVKNVGS GTYVA VK+P G+ VSVE
Sbjct: 178 KPFVCAKSFAIIDLNYPSISIPKLTIDGPVTINRRVKNVGSPGTYVAHVKVPEGIAVSVE 237

Query: 121 PSTLQFSSVGEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVKLG 174
           P+TLQF++VGEEKAFKVV +H G+ Q  GYVFGTL+WSDGKHF+RSPIA+KLG
Sbjct: 238 PNTLQFNNVGEEKAFKVVFRHIGQGQHQGYVFGTLIWSDGKHFLRSPIAMKLG 290

[Close]

BLAST of Sgr022086 vs. NCBI nr
Match: XP_038905686.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])

HSP 1 Score: 275.4 bits (703), Expect = 3.3e-70
Identity = 131/171 (76.61%), Postives = 147/171 (85.96%), Query Frame = 0


Query: 3   SILDFTGGKATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNKP 62
           +I D    KATP +YGAGHVHPN+AMDPGLVYD T+DDYLNFLCARGYN   +K+F NKP
Sbjct: 596 TISDTAKMKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSVALKKFYNKP 655

Query: 63  FTCAKSFALTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARVKMPLGVVVSVEPS 122
           F CAKSFA+TDLNYPSISVP L++GA VT+NRRVKNVG+ GTYVARVK    + VSVEPS
Sbjct: 656 FICAKSFAITDLNYPSISVPELRIGAPVTVNRRVKNVGTPGTYVARVKASPAISVSVEPS 715

Query: 123 TLQFSSVGEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVKLG 174
           TLQF+SVGEEKAFKVV Q+ GK QR G+VFGTL+WSDGKHFVRSPIAVKLG
Sbjct: 716 TLQFNSVGEEKAFKVVFQYKGKEQRQGHVFGTLIWSDGKHFVRSPIAVKLG 766

[Close]

BLAST of Sgr022086 vs. NCBI nr
Match: XP_023005962.1 (uncharacterized protein LOC111498820 [Cucurbita maxima])

HSP 1 Score: 274.2 bits (700), Expect = 7.5e-70
Identity = 128/171 (74.85%), Postives = 145/171 (84.80%), Query Frame = 0


Query: 2   HSILDFTGGKATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNK 61
           +++LDF   KATP +YGAGHVHPN+AMDPGLVYD T+DDYLNFLC +GYN   +K+FSNK
Sbjct: 590 NTMLDFNKVKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNK 649

Query: 62  PFTCAKSFALTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARVKMPLGVVVSVEP 121
           PF CAK+FA TDLNYPSISVP LQ+G  VT+NRRVKNVGS GTYVARV+MP G+ V VEP
Sbjct: 650 PFVCAKNFASTDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEP 709

Query: 122 STLQFSSVGEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVKL 173
           STLQF SVGEEKAFK+V  +  KV+R GYVFG LVWSDGKHFVRSPIAV L
Sbjct: 710 STLQFHSVGEEKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNL 760

[Close]

BLAST of Sgr022086 vs. NCBI nr
Match: KAG6596783.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 273.5 bits (698), Expect = 1.3e-69
Identity = 128/171 (74.85%), Postives = 145/171 (84.80%), Query Frame = 0


Query: 2   HSILDFTGGKATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNK 61
           +++LDF   KATP +YGAG VHPNNAMDPGLVYD T+DDYLNFLC +GYN   +K+FSNK
Sbjct: 595 NTMLDFNKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNK 654

Query: 62  PFTCAKSFALTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARVKMPLGVVVSVEP 121
           PF CAK+FA+TDLNYPSISVP LQ+GA VT+NRRVKNVGS GTYVARV+MP G+ V VEP
Sbjct: 655 PFVCAKNFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEP 714

Query: 122 STLQFSSVGEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVKL 173
           STLQF SVGEEK FK+V  +  KV+R GYVFG LVWSDGKHFVRSPIAV L
Sbjct: 715 STLQFHSVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNL 765

[Close]

BLAST of Sgr022086 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 1.7e-45
Identity = 94/170 (55.29%), Postives = 117/170 (68.82%), Query Frame = 0


Query: 4   ILDFTGGKATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNKPF 63
           I + T  KATP  +GAGHV PN A++PGLVYD  + DYLNFLC+ GYN +QI  FS   F
Sbjct: 604 IQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNF 663

Query: 64  TCAK-SFALTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARVKMPLGVVVSVEPS 123
           TC+    +L +LNYPSI+VPNL   + VT++R VKNVG    Y  +V  P GV V+V+P+
Sbjct: 664 TCSSPKISLVNLNYPSITVPNL-TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPT 723

Query: 124 TLQFSSVGEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVKL 173
           +L F+ VGE+K FKV+L  +      GYVFG LVWSD KH VRSPI VKL
Sbjct: 724 SLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

[Close]

BLAST of Sgr022086 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 2.4e-42
Identity = 88/170 (51.76%), Postives = 112/170 (65.88%), Query Frame = 0


Query: 4   ILDFTGGKATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNKP- 63
           ++D +  KA P  YG+GHV PN A  PGLVYD T  DYL+FLCA GYN T ++ F+  P 
Sbjct: 610 MVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQ 669

Query: 64  FTCAKSFALTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARVKMPLGVVVSVEPS 123
           +TC +   L D NYPSI+VPNL    ++T+ R++KNVG   TY AR + PLGV VSVEP 
Sbjct: 670 YTCRQGANLLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPK 729

Query: 124 TLQFSSVGEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVKL 173
            L F+  GE K F++ L+    V   GYVFG L W+D  H+VRSPI V+L
Sbjct: 730 QLTFNKTGEVKIFQMTLRPL-PVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

[Close]

BLAST of Sgr022086 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 161.8 bits (408), Expect = 7.1e-39
Identity = 80/161 (49.69%), Postives = 106/161 (65.84%), Query Frame = 0


Query: 12  ATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFS-NKPFTCAKSFA 71
           A    YG+GHV P+ A++PGLVYD ++ DYLNFLCA GY+Q  I   + N+ F C+ S +
Sbjct: 628 ADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQLISALNFNRTFICSGSHS 687

Query: 72  LTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARVKMPLGVVVSVEPSTLQFSSVG 131
           + DLNYPSI++PNL++   VTI R V NVG   TY    + P G  ++V P +L F+ +G
Sbjct: 688 VNDLNYPSITLPNLRL-KPVTIARTVTNVGPPSTYTVSTRSPNGYSIAVVPPSLTFTKIG 747

Query: 132 EEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVK 172
           E K FKV++Q +    R  Y FG L W+DGKH VRSPI VK
Sbjct: 748 ERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVRSPITVK 787

[Close]

BLAST of Sgr022086 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.5e-33
Identity = 74/171 (43.27%), Postives = 101/171 (59.06%), Query Frame = 0


Query: 4   ILDFTGGK-ATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNKP 63
           +LD   GK +TP ++GAGHV P  A +PGL+YD T +DYL FLCA  Y   QI+  S + 
Sbjct: 585 LLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRN 644

Query: 64  FTC--AKSFALTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARV-KMPLGVVVSV 123
           +TC  +KS+++ DLNYPS +V N+         R V +VG  GTY  +V     GV +SV
Sbjct: 645 YTCDPSKSYSVADLNYPSFAV-NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISV 704

Query: 124 EPSTLQFSSVGEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAV 171
           EP+ L F    E+K++ V          G   FG++ WSDGKH V SP+A+
Sbjct: 705 EPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

[Close]

BLAST of Sgr022086 vs. ExPASy Swiss-Prot
Match: F4JJH5 (Subtilisin-like protease SBT3.12 OS=Arabidopsis thaliana OX=3702 GN=SBT3.12 PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 7.8e-30
Identity = 70/163 (42.94%), Postives = 99/163 (60.74%), Query Frame = 0


Query: 12  ATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNKPFTCAKSF-A 71
           A P +YGAG V+   A DPGLVYD  +DDY+++ CA GYN T I   + KP  C+    +
Sbjct: 590 ADPFDYGAGLVNAERAKDPGLVYDMNIDDYIHYFCATGYNDTSITIITGKPTKCSSPLPS 649

Query: 72  LTDLNYPSISVPNLQMGATVTINRRVKNVGSV-GTYVARVKMPLGVVVSVEPSTLQFSSV 131
           + DLNYP+I++P+L+    VT+ R V NVG V   Y A V+ P GV + VEP TL F S 
Sbjct: 650 ILDLNYPAITIPDLE--EEVTVTRTVTNVGPVDSVYRAVVEPPRGVEIVVEPETLVFCSN 709

Query: 132 GEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVKL 173
            ++  FKV +  + K    G+ FG+  W+DG   V  P++V++
Sbjct: 710 TKKLGFKVRVSSSHK-SNTGFFFGSFTWTDGTRNVTIPLSVRI 749

[Close]

BLAST of Sgr022086 vs. ExPASy TrEMBL
Match: A0A6J1CQW4 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111013336 PE=3 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 8.3e-75
Identity = 138/171 (80.70%), Postives = 153/171 (89.47%), Query Frame = 0


Query: 3   SILDFTGGKATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNKP 62
           SILDFT GKA+PLEYGAGHV PN+AMDPGLVYD T+DDYLN+LCARG NQTQ++QFSNKP
Sbjct: 596 SILDFTKGKASPLEYGAGHVQPNDAMDPGLVYDTTIDDYLNYLCARGANQTQLRQFSNKP 655

Query: 63  FTCAKSFALTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARVKMPLGVVVSVEPS 122
           FTCAKSFALTDLNYPSI+VPNL++GA  T+NRRVKNVGS GTY ARVK PLGV V VEPS
Sbjct: 656 FTCAKSFALTDLNYPSIAVPNLKVGAPTTVNRRVKNVGSPGTYTARVKAPLGVSVVVEPS 715

Query: 123 TLQFSSVGEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVKLG 174
           TLQF + GEEKAFKVVLQ T K ++G Y+FGT+VWSDGKHFVRSPIAVK G
Sbjct: 716 TLQFRTAGEEKAFKVVLQTTPKAEQGVYLFGTMVWSDGKHFVRSPIAVKSG 766

[Close]

BLAST of Sgr022086 vs. ExPASy TrEMBL
Match: A0A6J1KUL8 (uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.6e-70
Identity = 128/171 (74.85%), Postives = 145/171 (84.80%), Query Frame = 0


Query: 2   HSILDFTGGKATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNK 61
           +++LDF   KATP +YGAGHVHPN+AMDPGLVYD T+DDYLNFLC +GYN   +K+FSNK
Sbjct: 590 NTMLDFNKVKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNK 649

Query: 62  PFTCAKSFALTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARVKMPLGVVVSVEP 121
           PF CAK+FA TDLNYPSISVP LQ+G  VT+NRRVKNVGS GTYVARV+MP G+ V VEP
Sbjct: 650 PFVCAKNFASTDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEP 709

Query: 122 STLQFSSVGEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVKL 173
           STLQF SVGEEKAFK+V  +  KV+R GYVFG LVWSDGKHFVRSPIAV L
Sbjct: 710 STLQFHSVGEEKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNL 760

[Close]

BLAST of Sgr022086 vs. ExPASy TrEMBL
Match: A0A6J1GDW2 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453052 PE=3 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.4e-69
Identity = 128/171 (74.85%), Postives = 143/171 (83.63%), Query Frame = 0


Query: 2   HSILDFTGGKATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNK 61
           ++ILD+T  KATP +YGAGHVHPNNAMDPGLVYD TVDDYLNFLC RGYN   +K+FSNK
Sbjct: 602 NTILDYTKVKATPFDYGAGHVHPNNAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNK 661

Query: 62  PFTCAKSFALTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARVKMPLGVVVSVEP 121
           PF CAK+FA TD NYPSI VP LQ+G +VT+NRRVKNVGS GTYVARVKMP G+ V VEP
Sbjct: 662 PFVCAKNFATTDFNYPSILVPRLQIGESVTVNRRVKNVGSAGTYVARVKMPKGITVMVEP 721

Query: 122 STLQFSSVGEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVKL 173
           STLQF SVGEEK FK+V  +  K++R GYVFG LVWSDGKHFVRS IAV L
Sbjct: 722 STLQFHSVGEEKPFKLVFHYAHKLRRQGYVFGALVWSDGKHFVRSSIAVNL 772

[Close]

BLAST of Sgr022086 vs. ExPASy TrEMBL
Match: A0A6J1GDR2 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453063 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 1.2e-68
Identity = 125/171 (73.10%), Postives = 144/171 (84.21%), Query Frame = 0


Query: 2   HSILDFTGGKATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNK 61
           +++LDF   KATP +YGAG VHPNNAMDPGLVYD T+DDYLNFLC +GYN   +K+FSNK
Sbjct: 595 NTMLDFNKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNK 654

Query: 62  PFTCAKSFALTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARVKMPLGVVVSVEP 121
           PF C+K+FA+TDLNYPSISVP LQ+GA VT+NRRVKNVGS GTYVARV+MP G+ V VEP
Sbjct: 655 PFVCSKNFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEP 714

Query: 122 STLQFSSVGEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVKL 173
           STLQF  VGEEK FK+V  +  +V+R GYVFG LVWSDGKHFVRSPIAV L
Sbjct: 715 STLQFHGVGEEKPFKLVFHYAQQVRRPGYVFGALVWSDGKHFVRSPIAVNL 765

[Close]

BLAST of Sgr022086 vs. ExPASy TrEMBL
Match: A0A5D3D732 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00170 PE=3 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.7e-67
Identity = 123/173 (71.10%), Postives = 146/173 (84.39%), Query Frame = 0


Query: 1    MHSILDFTGGKATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSN 60
            M SILD+   KATP +YGAGHVHPNNA+DPGLVYD T++DY+NF+CA+GYN T +K+F N
Sbjct: 1310 MKSILDYNKAKATPFQYGAGHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKRFYN 1369

Query: 61   KPFTCAKSFALTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARVKMPLGVVVSVE 120
            KP+ C KSF LTDLNYPSISVP L +G  VTINRR+KNVG+ GTYVARVK+   V V+V+
Sbjct: 1370 KPYLCPKSFPLTDLNYPSISVPKLTIGVPVTINRRLKNVGTPGTYVARVKVSSKVSVTVK 1429

Query: 121  PSTLQFSSVGEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVKLG 174
            PSTLQF+SVGEEKAFKVV ++ GK Q  G+VFGTL+WSDGKHFVRSP+ VKLG
Sbjct: 1430 PSTLQFNSVGEEKAFKVVFEYKGKGQGKGHVFGTLIWSDGKHFVRSPMVVKLG 1482

[Close]

BLAST of Sgr022086 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 183.7 bits (465), Expect = 1.2e-46
Identity = 94/170 (55.29%), Postives = 117/170 (68.82%), Query Frame = 0


Query: 4   ILDFTGGKATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNKPF 63
           I + T  KATP  +GAGHV PN A++PGLVYD  + DYLNFLC+ GYN +QI  FS   F
Sbjct: 604 IQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNF 663

Query: 64  TCAK-SFALTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARVKMPLGVVVSVEPS 123
           TC+    +L +LNYPSI+VPNL   + VT++R VKNVG    Y  +V  P GV V+V+P+
Sbjct: 664 TCSSPKISLVNLNYPSITVPNL-TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPT 723

Query: 124 TLQFSSVGEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVKL 173
           +L F+ VGE+K FKV+L  +      GYVFG LVWSD KH VRSPI VKL
Sbjct: 724 SLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

[Close]

BLAST of Sgr022086 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 173.3 bits (438), Expect = 1.7e-43
Identity = 88/170 (51.76%), Postives = 112/170 (65.88%), Query Frame = 0


Query: 4   ILDFTGGKATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNKP- 63
           ++D +  KA P  YG+GHV PN A  PGLVYD T  DYL+FLCA GYN T ++ F+  P 
Sbjct: 610 MVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQ 669

Query: 64  FTCAKSFALTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARVKMPLGVVVSVEPS 123
           +TC +   L D NYPSI+VPNL    ++T+ R++KNVG   TY AR + PLGV VSVEP 
Sbjct: 670 YTCRQGANLLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPK 729

Query: 124 TLQFSSVGEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVKL 173
            L F+  GE K F++ L+    V   GYVFG L W+D  H+VRSPI V+L
Sbjct: 730 QLTFNKTGEVKIFQMTLRPL-PVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

[Close]

BLAST of Sgr022086 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 144.1 bits (362), Expect = 1.1e-34
Identity = 74/171 (43.27%), Postives = 101/171 (59.06%), Query Frame = 0


Query: 4   ILDFTGGK-ATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNKP 63
           +LD   GK +TP ++GAGHV P  A +PGL+YD T +DYL FLCA  Y   QI+  S + 
Sbjct: 585 LLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRN 644

Query: 64  FTC--AKSFALTDLNYPSISVPNLQMGATVTINRRVKNVGSVGTYVARV-KMPLGVVVSV 123
           +TC  +KS+++ DLNYPS +V N+         R V +VG  GTY  +V     GV +SV
Sbjct: 645 YTCDPSKSYSVADLNYPSFAV-NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISV 704

Query: 124 EPSTLQFSSVGEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAV 171
           EP+ L F    E+K++ V          G   FG++ WSDGKH V SP+A+
Sbjct: 705 EPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

[Close]

BLAST of Sgr022086 vs. TAIR 10
Match: AT4G21326.1 (subtilase 3.12 )

HSP 1 Score: 131.7 bits (330), Expect = 5.6e-31
Identity = 70/163 (42.94%), Postives = 99/163 (60.74%), Query Frame = 0


Query: 12  ATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNKPFTCAKSF-A 71
           A P +YGAG V+   A DPGLVYD  +DDY+++ CA GYN T I   + KP  C+    +
Sbjct: 590 ADPFDYGAGLVNAERAKDPGLVYDMNIDDYIHYFCATGYNDTSITIITGKPTKCSSPLPS 649

Query: 72  LTDLNYPSISVPNLQMGATVTINRRVKNVGSV-GTYVARVKMPLGVVVSVEPSTLQFSSV 131
           + DLNYP+I++P+L+    VT+ R V NVG V   Y A V+ P GV + VEP TL F S 
Sbjct: 650 ILDLNYPAITIPDLE--EEVTVTRTVTNVGPVDSVYRAVVEPPRGVEIVVEPETLVFCSN 709

Query: 132 GEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVKL 173
            ++  FKV +  + K    G+ FG+  W+DG   V  P++V++
Sbjct: 710 TKKLGFKVRVSSSHK-SNTGFFFGSFTWTDGTRNVTIPLSVRI 749

[Close]

BLAST of Sgr022086 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 125.9 bits (315), Expect = 3.1e-29
Identity = 69/162 (42.59%), Postives = 98/162 (60.49%), Query Frame = 0


Query: 12  ATPLEYGAGHVHPNNAMDPGLVYDATVDDYLNFLCARGYNQTQIKQFSNKPFTCAK-SFA 71
           A P +YG G V+P  + +PGLVYD  ++DY+ ++C+ GYN+T I Q   K   C+    +
Sbjct: 609 ADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSISQLIGKTTVCSNPKPS 668

Query: 72  LTDLNYPSISVPNLQMGATVTINRRVKNVGSVGT-YVARVKMPLGVVVSVEPSTLQFSSV 131
           + D N PSI++PNL+    VTI R V NVG + + Y   V+ PLG  V+V P TL F+S 
Sbjct: 669 VLDFNLPSITIPNLK--DEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNST 728

Query: 132 GEEKAFKVVLQHTGKVQRGGYVFGTLVWSDGKHFVRSPIAVK 172
            ++  FKV +  T K    GY FG+L WSD  H V  P++V+
Sbjct: 729 TKKVYFKVKVSTTHKTNT-GYYFGSLTWSDSLHNVTIPLSVR 767

The following BLAST results are available for this feature:

BLAST of Sgr022086 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143462.1	1.7e-74	80.70	subtilisin-like protease SBT5.4 [Momordica charantia]	[more]
XP_038904202.1	2.6e-70	73.99	subtilisin-like protease SBT5.3 [Benincasa hispida]	[more]
XP_038905686.1	3.3e-70	76.61	subtilisin-like protease SBT5.3 [Benincasa hispida]	[more]
XP_023005962.1	7.5e-70	74.85	uncharacterized protein LOC111498820 [Cucurbita maxima]	[more]
KAG6596783.1	1.3e-69	74.85	Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Sgr022086 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZSP5	1.7e-45	55.29	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=...	[more]
F4JXC5	2.4e-42	51.76	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S...	[more]
I1N462	7.1e-39	49.69	Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P...	[more]
O65351	1.5e-33	43.27	Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S...	[more]
F4JJH5	7.8e-30	42.94	Subtilisin-like protease SBT3.12 OS=Arabidopsis thaliana OX=3702 GN=SBT3.12 PE=3...	[more]

BLAST of Sgr022086 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQW4	8.3e-75	80.70	subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111013336 P...	[more]
A0A6J1KUL8	3.6e-70	74.85	uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820...	[more]
A0A6J1GDW2	1.4e-69	74.85	subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453052 PE...	[more]
A0A6J1GDR2	1.2e-68	73.10	subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453063 PE...	[more]
A0A5D3D732	1.7e-67	71.10	Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]

BLAST of Sgr022086 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G04160.1	1.2e-46	55.29	Subtilisin-like serine endopeptidase family protein	[more]
AT5G59810.1	1.7e-43	51.76	Subtilase family protein	[more]
AT5G67360.1	1.1e-34	43.27	Subtilase family protein	[more]
AT4G21326.1	5.6e-31	42.94	subtilase 3.12	[more]
AT4G10550.1	3.1e-29	42.59	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 73..170 e-value: 2.3E-29 score: 101.5
None	No IPR available	GENE3D	2.60.40.2310		coord: 45..172 e-value: 1.5E-39 score: 136.7
None	No IPR available	PANTHER	PTHR43806	PEPTIDASE S8	coord: 2..171
None	No IPR available	PANTHER	PTHR43806:SF34	SUBTILISIN-LIKE PROTEASE SBT5.4	coord: 2..171
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 8..41 e-value: 9.6E-9 score: 36.5

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr022086.1	Sgr022086.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009610	response to symbiotic fungus
molecular_function	GO:0004252	serine-type endopeptidase activity