Sgr021061 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr021061
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionDNA ligase
Locationtig00153639: 640172 .. 642496 (+)
RNA-Seq ExpressionSgr021061
SyntenySgr021061
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCCAATCTTCCACACCTAAACACCCATCGATTGACTCTTCCCTCTCCTTCGTCACTGCTATCGAATCGTTTTCACGGCATTCACATCTGCCCCTTCTTCCGCCGACGCTTCCATTCCGTCCCCCTCCTTTTCCGTCCGACTTCCCGCAATCCAAACTGATCCCAAAATCCCGTTTCGTCGTCGACGCATTTGGTTACGCCGGTGATTACTCTGTTTCCTACTTCCTCTCTCATTTTCACTCCGATCACTATGGCGGTCTCTCCCCCAGTTGGTGCAAAGGTATTATCTTCTGCTCTGACACCACTGCCGGCCTTCTGGTCGAAGTTCTCAATGTCCCTTCGCCGTTTGTACAGCCATTGCCGCTTCGCGAGCCCGTAGCAATCGATGGCTGTGAGGTAATACTAATTGATGCGAATCACTGTCCCGGTGCGGTGCAGTTCTTGTTTAAGATTCCAAGTGGGGATGGAAAGTTAGAGAGATATGTTCATACTGGTGATTTTCGGTTTTGTGATTCTATGAGAGGCGATCCTGTTTTGGAAACATTTATTGGCTCGGATGCAATTTTCTTGGACACTACGTACTGTAACCCGAAATTTATTTTTCCTTCCCAGGAGGAGTCTATCGAATATATAGTACACGTCATACAAAAAATTGGAGGTGCAAGTAAGGGTTCTATGGGGGATGTTCTTTTCCTTGTAGCAACTTACGTAATAGGAAAAGAAAAGATTTTGCTTCAAATTGCAAGAAGATGTAACCGTAAGATAATGGTGGATGCTAGAAAAATGAAAGTTTTACGGGTTTTGGGACATGGTGAGAGCGGGGTGTTTACTGAGCATGAAAGTGAAAGTGATGTTCACGTTGTTGGATGGAATGTTTTGGGTGAGACTTGGCCTTATTTTAGGCCCAATTTTGTTAAAATGAATGATATGATGGTGGAAAAGGGGTATTCTAAGGTTGTCGGTTTTGTTCCGACAGGGTGGACTTATGAGGTAAAGCGCAACAAGTTCTCAGTAAGGACCAAGGATTCTCTTGAGATACATCTTGTGCCATACAGTGAACATTCAAACTATAATGAACTTAGGGAGTATGTGAAGTTTTTGAAACCAAAAAGGGTTGTTCCCACTGTAGGTTTAGATGTTGATAAACTTGACAGCAAGCATGCTAATAAAATTAAGAAGCATTTTGCAGGTTTAGTTGATGAAATGGCGAACAGAAAAGAATTCTTAAGGGGCTTTCATCGTGGATCCAGTGAAATGAGTGATGAAGTTGATGATAATGCCACAAAAGCTGTAAATGAGTGCCAGGACTTTAAAGAGAAGGAGATTTCATTGTGTATGAATATAAGTGTCAAAACAGAAGCATGCAGTAGTGGTATGGGGTTGTCTTCTCTACAAGAAGTTGGTTCTACAGGTCTAACTGTGTCAAATGATGAGGAACAGAAGAAGATTATCCAGGAACTTCGTGAGTGCCTGCCCGCATGGGTCACTCAGGATCAGATGTTAGATCTGATTAGCAGCTCAGGGAGAAATGTTGTTGATGCAGTTTCTATTTTCTATGAACGAGAAACTGAATTTTGTGAACATGTTACTACTTTCACGGATAATCTTCCCAAGTTCCAGACCAAAGTACTTGATGACCCATTTACTTTTCCCACTACAGATATGAAAAAGAAGATTGTTTTGGAAAATTTTGACATTTCTTCAAGCCAAGACTCAAAGTCATCCAGTACAAGGTGCATAACCAAGAGTAATGCTTCTCCTGGAAAAAGGAAGAGAAACATTCAGGAGAGGCCAAAAAATAAGTCAAAAAATAATTCAAGTTTACAATCTGGTGGGTCAAAGCAATCTACAATAACAAAGTTCTTCAGGAAAGCTTCAAATTTGTCAGAAGGTGCTAAAATGGAATCTACAGTGGAGCAAAGTTGTCAATTTCAAAGTTCTCCACATGATATTACCAAAAATGCAAACAAGATAGACAGGTTTATTGAGATAATAAATGGTGATGAGTCATTAAAAGAACTTGTTGCATCATTACTAGTGGAAACAAATGGAGACATGAATATGGCGCTAGATTTGTATTATGCTAATCCTCAGGGCAGGCCTGTTCAGAATGAAAAGGGTCACATCTTCAGTGGGAACCCAGTCCAAGCTGAAAGCAATTTGGATGAATGCTTATCTACCCAAAAGGAAAACATGCGAGAAAATTTTGGACTGGAGACTGATTTGTCTAAGCTGTCAGGTTCAACGAAGAAGATTAATGAACCTCTTTTATCCTTACCACCTGAAAAGTATGACCCGGTTGAGCATGGTTTGTATTAG

mRNA sequence

ATGGCTTCCCAATCTTCCACACCTAAACACCCATCGATTGACTCTTCCCTCTCCTTCGTCACTGCTATCGAATCGTTTTCACGGCATTCACATCTGCCCCTTCTTCCGCCGACGCTTCCATTCCGTCCCCCTCCTTTTCCGTCCGACTTCCCGCAATCCAAACTGATCCCAAAATCCCGTTTCGTCGTCGACGCATTTGGTTACGCCGGTGATTACTCTGTTTCCTACTTCCTCTCTCATTTTCACTCCGATCACTATGGCGGTCTCTCCCCCAGTTGGTGCAAAGGTATTATCTTCTGCTCTGACACCACTGCCGGCCTTCTGGTCGAAGTTCTCAATGTCCCTTCGCCGTTTGTACAGCCATTGCCGCTTCGCGAGCCCGTAGCAATCGATGGCTGTGAGGTAATACTAATTGATGCGAATCACTGTCCCGGTGCGGTGCAGTTCTTGTTTAAGATTCCAAGTGGGGATGGAAAGTTAGAGAGATATGTTCATACTGGTGATTTTCGGTTTTGTGATTCTATGAGAGGCGATCCTGTTTTGGAAACATTTATTGGCTCGGATGCAATTTTCTTGGACACTACGTACTGTAACCCGAAATTTATTTTTCCTTCCCAGGAGGAGTCTATCGAATATATAGTACACGTCATACAAAAAATTGGAGGTGCAAGTAAGGGTTCTATGGGGGATGTTCTTTTCCTTGTAGCAACTTACGTAATAGGAAAAGAAAAGATTTTGCTTCAAATTGCAAGAAGATGTAACCGTAAGATAATGGTGGATGCTAGAAAAATGAAAGTTTTACGGGTTTTGGGACATGGTGAGAGCGGGGTGTTTACTGAGCATGAAAGTGAAAGTGATGTTCACGTTGTTGGATGGAATGTTTTGGGTGAGACTTGGCCTTATTTTAGGCCCAATTTTGTTAAAATGAATGATATGATGGTGGAAAAGGGGTATTCTAAGGTTGTCGGTTTTGTTCCGACAGGGTGGACTTATGAGGTAAAGCGCAACAAGTTCTCAGTAAGGACCAAGGATTCTCTTGAGATACATCTTGTGCCATACAGTGAACATTCAAACTATAATGAACTTAGGGAGTATGTGAAGTTTTTGAAACCAAAAAGGGTTGTTCCCACTGTAGGTTTAGATGTTGATAAACTTGACAGCAAGCATGCTAATAAAATTAAGAAGCATTTTGCAGGTTTAGTTGATGAAATGGCGAACAGAAAAGAATTCTTAAGGGGCTTTCATCGTGGATCCAGTGAAATGAGTGATGAAGTTGATGATAATGCCACAAAAGCTGTAAATGAGTGCCAGGACTTTAAAGAGAAGGAGATTTCATTGTGTATGAATATAAGTGTCAAAACAGAAGCATGCAGTAGTGGTATGGGGTTGTCTTCTCTACAAGAAGTTGGTTCTACAGGTCTAACTGTGTCAAATGATGAGGAACAGAAGAAGATTATCCAGGAACTTCGTGAGTGCCTGCCCGCATGGGTCACTCAGGATCAGATGTTAGATCTGATTAGCAGCTCAGGGAGAAATGTTGTTGATGCAGTTTCTATTTTCTATGAACGAGAAACTGAATTTTGTGAACATGTTACTACTTTCACGGATAATCTTCCCAAGTTCCAGACCAAAGTACTTGATGACCCATTTACTTTTCCCACTACAGATATGAAAAAGAAGATTGTTTTGGAAAATTTTGACATTTCTTCAAGCCAAGACTCAAAGTCATCCAGTACAAGGTGCATAACCAAGAGTAATGCTTCTCCTGGAAAAAGGAAGAGAAACATTCAGGAGAGGCCAAAAAATAAGTCAAAAAATAATTCAAGTTTACAATCTGGTGGGTCAAAGCAATCTACAATAACAAAGTTCTTCAGGAAAGCTTCAAATTTGTCAGAAGGTGCTAAAATGGAATCTACAGTGGAGCAAAGTTGTCAATTTCAAAGTTCTCCACATGATATTACCAAAAATGCAAACAAGATAGACAGGTTTATTGAGATAATAAATGGTGATGAGTCATTAAAAGAACTTGTTGCATCATTACTAGTGGAAACAAATGGAGACATGAATATGGCGCTAGATTTGTATTATGCTAATCCTCAGGGCAGGCCTGTTCAGAATGAAAAGGGTCACATCTTCAGTGGGAACCCAGTCCAAGCTGAAAGCAATTTGGATGAATGCTTATCTACCCAAAAGGAAAACATGCGAGAAAATTTTGGACTGGAGACTGATTTGTCTAAGCTGTCAGGTTCAACGAAGAAGATTAATGAACCTCTTTTATCCTTACCACCTGAAAAGTATGACCCGGTTGAGCATGGTTTGTATTAG

Coding sequence (CDS)

ATGGCTTCCCAATCTTCCACACCTAAACACCCATCGATTGACTCTTCCCTCTCCTTCGTCACTGCTATCGAATCGTTTTCACGGCATTCACATCTGCCCCTTCTTCCGCCGACGCTTCCATTCCGTCCCCCTCCTTTTCCGTCCGACTTCCCGCAATCCAAACTGATCCCAAAATCCCGTTTCGTCGTCGACGCATTTGGTTACGCCGGTGATTACTCTGTTTCCTACTTCCTCTCTCATTTTCACTCCGATCACTATGGCGGTCTCTCCCCCAGTTGGTGCAAAGGTATTATCTTCTGCTCTGACACCACTGCCGGCCTTCTGGTCGAAGTTCTCAATGTCCCTTCGCCGTTTGTACAGCCATTGCCGCTTCGCGAGCCCGTAGCAATCGATGGCTGTGAGGTAATACTAATTGATGCGAATCACTGTCCCGGTGCGGTGCAGTTCTTGTTTAAGATTCCAAGTGGGGATGGAAAGTTAGAGAGATATGTTCATACTGGTGATTTTCGGTTTTGTGATTCTATGAGAGGCGATCCTGTTTTGGAAACATTTATTGGCTCGGATGCAATTTTCTTGGACACTACGTACTGTAACCCGAAATTTATTTTTCCTTCCCAGGAGGAGTCTATCGAATATATAGTACACGTCATACAAAAAATTGGAGGTGCAAGTAAGGGTTCTATGGGGGATGTTCTTTTCCTTGTAGCAACTTACGTAATAGGAAAAGAAAAGATTTTGCTTCAAATTGCAAGAAGATGTAACCGTAAGATAATGGTGGATGCTAGAAAAATGAAAGTTTTACGGGTTTTGGGACATGGTGAGAGCGGGGTGTTTACTGAGCATGAAAGTGAAAGTGATGTTCACGTTGTTGGATGGAATGTTTTGGGTGAGACTTGGCCTTATTTTAGGCCCAATTTTGTTAAAATGAATGATATGATGGTGGAAAAGGGGTATTCTAAGGTTGTCGGTTTTGTTCCGACAGGGTGGACTTATGAGGTAAAGCGCAACAAGTTCTCAGTAAGGACCAAGGATTCTCTTGAGATACATCTTGTGCCATACAGTGAACATTCAAACTATAATGAACTTAGGGAGTATGTGAAGTTTTTGAAACCAAAAAGGGTTGTTCCCACTGTAGGTTTAGATGTTGATAAACTTGACAGCAAGCATGCTAATAAAATTAAGAAGCATTTTGCAGGTTTAGTTGATGAAATGGCGAACAGAAAAGAATTCTTAAGGGGCTTTCATCGTGGATCCAGTGAAATGAGTGATGAAGTTGATGATAATGCCACAAAAGCTGTAAATGAGTGCCAGGACTTTAAAGAGAAGGAGATTTCATTGTGTATGAATATAAGTGTCAAAACAGAAGCATGCAGTAGTGGTATGGGGTTGTCTTCTCTACAAGAAGTTGGTTCTACAGGTCTAACTGTGTCAAATGATGAGGAACAGAAGAAGATTATCCAGGAACTTCGTGAGTGCCTGCCCGCATGGGTCACTCAGGATCAGATGTTAGATCTGATTAGCAGCTCAGGGAGAAATGTTGTTGATGCAGTTTCTATTTTCTATGAACGAGAAACTGAATTTTGTGAACATGTTACTACTTTCACGGATAATCTTCCCAAGTTCCAGACCAAAGTACTTGATGACCCATTTACTTTTCCCACTACAGATATGAAAAAGAAGATTGTTTTGGAAAATTTTGACATTTCTTCAAGCCAAGACTCAAAGTCATCCAGTACAAGGTGCATAACCAAGAGTAATGCTTCTCCTGGAAAAAGGAAGAGAAACATTCAGGAGAGGCCAAAAAATAAGTCAAAAAATAATTCAAGTTTACAATCTGGTGGGTCAAAGCAATCTACAATAACAAAGTTCTTCAGGAAAGCTTCAAATTTGTCAGAAGGTGCTAAAATGGAATCTACAGTGGAGCAAAGTTGTCAATTTCAAAGTTCTCCACATGATATTACCAAAAATGCAAACAAGATAGACAGGTTTATTGAGATAATAAATGGTGATGAGTCATTAAAAGAACTTGTTGCATCATTACTAGTGGAAACAAATGGAGACATGAATATGGCGCTAGATTTGTATTATGCTAATCCTCAGGGCAGGCCTGTTCAGAATGAAAAGGGTCACATCTTCAGTGGGAACCCAGTCCAAGCTGAAAGCAATTTGGATGAATGCTTATCTACCCAAAAGGAAAACATGCGAGAAAATTTTGGACTGGAGACTGATTTGTCTAAGCTGTCAGGTTCAACGAAGAAGATTAATGAACCTCTTTTATCCTTACCACCTGAAAAGTATGACCCGGTTGAGCATGGTTTGTATTAG

Protein sequence

MASQSSTPKHPSIDSSLSFVTAIESFSRHSHLPLLPPTLPFRPPPFPSDFPQSKLIPKSRFVVDAFGYAGDYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLVEVLNVPSPFVQPLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVHTGDFRFCDSMRGDPVLETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASKGSMGDVLFLVATYVIGKEKILLQIARRCNRKIMVDARKMKVLRVLGHGESGVFTEHESESDVHVVGWNVLGETWPYFRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNKFSVRTKDSLEIHLVPYSEHSNYNELREYVKFLKPKRVVPTVGLDVDKLDSKHANKIKKHFAGLVDEMANRKEFLRGFHRGSSEMSDEVDDNATKAVNECQDFKEKEISLCMNISVKTEACSSGMGLSSLQEVGSTGLTVSNDEEQKKIIQELRECLPAWVTQDQMLDLISSSGRNVVDAVSIFYERETEFCEHVTTFTDNLPKFQTKVLDDPFTFPTTDMKKKIVLENFDISSSQDSKSSSTRCITKSNASPGKRKRNIQERPKNKSKNNSSLQSGGSKQSTITKFFRKASNLSEGAKMESTVEQSCQFQSSPHDITKNANKIDRFIEIINGDESLKELVASLLVETNGDMNMALDLYYANPQGRPVQNEKGHIFSGNPVQAESNLDECLSTQKENMRENFGLETDLSKLSGSTKKINEPLLSLPPEKYDPVEHGLY
Homology
BLAST of Sgr021061 vs. NCBI nr
Match: XP_022158502.1 (DNA ligase 6-like isoform X2 [Momordica charantia])

HSP 1 Score: 1325.1 bits (3428), Expect = 0.0e+00
Identity = 673/796 (84.55%), Postives = 718/796 (90.20%), Query Frame = 0

Query: 1   MASQSSTPKHPSIDSSLSFVTAIESFSRHSHLPLLPPTLPFRPPPFPSDFPQSKLIPKSR 60
           MAS S T  H +IDSSLSFVTAIE FSRHSHLPLLPP LPFRPPPFPSDFPQSKLIPKSR
Sbjct: 1   MASDSPTHIHQTIDSSLSFVTAIELFSRHSHLPLLPPPLPFRPPPFPSDFPQSKLIPKSR 60

Query: 61  FVVDAFGYAGDYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLVEVLNVPSPFVQ 120
           FVVDAFG AGDYSVSYFLSHFHSDHYGGLS SWCKGIIFCSDTTA LL+EVL VPSPFV+
Sbjct: 61  FVVDAFGLAGDYSVSYFLSHFHSDHYGGLSISWCKGIIFCSDTTARLLLEVLKVPSPFVR 120

Query: 121 PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVHTGDFRFCDSMRGDPV 180
           PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIP GDGKLERYVHTGDFRFCDSMR DP+
Sbjct: 121 PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPGGDGKLERYVHTGDFRFCDSMRVDPI 180

Query: 181 LETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASKGSMGDVLFLVATYVI 240
           LE FIGSDAIFLDTTYCNPKFIFP+QEESIEYIV++IQKIGGASKGSMG+VLFLVATYVI
Sbjct: 181 LEEFIGSDAIFLDTTYCNPKFIFPTQEESIEYIVNIIQKIGGASKGSMGNVLFLVATYVI 240

Query: 241 GKEKILLQIARRCNRKIMVDARKMKVLRVLGHGESGVFTEHESESDVHVVGWNVLGETWP 300
           GKEKILL+IA RCNRKIMVDARKM VLRVLGHGESGVFTE ESESDVHVVGWN+LG+TWP
Sbjct: 241 GKEKILLEIASRCNRKIMVDARKMTVLRVLGHGESGVFTELESESDVHVVGWNILGDTWP 300

Query: 301 YFRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNKFSVRTKDSLEIHLVPYSEHSNYN 360
           YFRPNFVKMND+MVEKGYSKVVGFVPTGWTYEVKRNKFSVR+KDSLEIHLVPYSEHSNYN
Sbjct: 301 YFRPNFVKMNDIMVEKGYSKVVGFVPTGWTYEVKRNKFSVRSKDSLEIHLVPYSEHSNYN 360

Query: 361 ELREYVKFLKPKRVVPTVGLDVDKLDSKHANKIKKHFAGLVDEMANRKEFLRGFHRGSSE 420
           ELREYVKFL+P+RVVPTVG DVDKLDSKH NKIKKHFAGLVDEMANRKEFL+GFHRGSSE
Sbjct: 361 ELREYVKFLRPRRVVPTVGSDVDKLDSKHTNKIKKHFAGLVDEMANRKEFLKGFHRGSSE 420

Query: 421 MSDEVDDNATKAVNECQDFKEKEISLCMNISVKTEACSSGMGLSSLQEVGSTGLTVSNDE 480
           MSD+V DN  KAVNE +D +EKEIS CM +SVKTEACSSGMG SSL+EVGST L   NDE
Sbjct: 421 MSDDVYDNDAKAVNEFRDLEEKEISSCMKMSVKTEACSSGMGFSSLKEVGSTDL---NDE 480

Query: 481 EQKKIIQELRECLPAWVTQDQMLDLISSSGRNVVDAVSIFYERETEFCEHVTTFTDNLPK 540
           +++KIIQELRECLPAWVTQDQMLDLISSSGRN+VDAVS FYERETEFCEH+TT T+NLPK
Sbjct: 481 DKRKIIQELRECLPAWVTQDQMLDLISSSGRNLVDAVSNFYERETEFCEHITTLTENLPK 540

Query: 541 FQTKVLDDPFTFPTTDMKKKIVLENFDISSSQDSKSSSTRCITKSNASPGKRKRNIQERP 600
           FQ+KVLD PFTF TTD+KKKIVL  F+ISSSQDSK+SS RC+ KSNASPGKRKRNIQE+P
Sbjct: 541 FQSKVLDVPFTFHTTDIKKKIVLGKFEISSSQDSKASSPRCMAKSNASPGKRKRNIQEKP 600

Query: 601 KNKSKNNS----------------------SLQSGGSKQSTITKFFRKASNLSEGAKMES 660
           +N+SK NS                      SLQSGGSKQSTITKFFRKAS+LSEG KMES
Sbjct: 601 QNRSKKNSSLQSSGSKKKSSLQSSGSKKILSLQSGGSKQSTITKFFRKASHLSEGDKMES 660

Query: 661 TVEQSCQFQSSPHDITKNANKIDRFIEIINGDESLKELVASLLVETNGDMNMALDLYYAN 720
           TVEQSCQ QS+ +DI K+ NKIDRFI+IINGDESLK+LV SLLVETNGD+NMALDLYYAN
Sbjct: 661 TVEQSCQDQSTSYDINKDTNKIDRFIQIINGDESLKQLVTSLLVETNGDINMALDLYYAN 720

Query: 721 PQGRPVQNEKGHIFSGNPVQAESNLDECLSTQKENMRENFGLETDLSKLSGSTKKINEPL 775
           PQ R  QNEKGHIFSGNP+Q ESNLDECLSTQKE M ENFGLETD SKLS STKKINEPL
Sbjct: 721 PQERLGQNEKGHIFSGNPIQIESNLDECLSTQKEKMAENFGLETDFSKLSSSTKKINEPL 780

BLAST of Sgr021061 vs. NCBI nr
Match: XP_022158501.1 (DNA ligase 6-like isoform X1 [Momordica charantia])

HSP 1 Score: 1325.1 bits (3428), Expect = 0.0e+00
Identity = 673/796 (84.55%), Postives = 718/796 (90.20%), Query Frame = 0

Query: 1   MASQSSTPKHPSIDSSLSFVTAIESFSRHSHLPLLPPTLPFRPPPFPSDFPQSKLIPKSR 60
           MAS S T  H +IDSSLSFVTAIE FSRHSHLPLLPP LPFRPPPFPSDFPQSKLIPKSR
Sbjct: 1   MASDSPTHIHQTIDSSLSFVTAIELFSRHSHLPLLPPPLPFRPPPFPSDFPQSKLIPKSR 60

Query: 61  FVVDAFGYAGDYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLVEVLNVPSPFVQ 120
           FVVDAFG AGDYSVSYFLSHFHSDHYGGLS SWCKGIIFCSDTTA LL+EVL VPSPFV+
Sbjct: 61  FVVDAFGLAGDYSVSYFLSHFHSDHYGGLSISWCKGIIFCSDTTARLLLEVLKVPSPFVR 120

Query: 121 PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVHTGDFRFCDSMRGDPV 180
           PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIP GDGKLERYVHTGDFRFCDSMR DP+
Sbjct: 121 PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPGGDGKLERYVHTGDFRFCDSMRVDPI 180

Query: 181 LETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASKGSMGDVLFLVATYVI 240
           LE FIGSDAIFLDTTYCNPKFIFP+QEESIEYIV++IQKIGGASKGSMG+VLFLVATYVI
Sbjct: 181 LEEFIGSDAIFLDTTYCNPKFIFPTQEESIEYIVNIIQKIGGASKGSMGNVLFLVATYVI 240

Query: 241 GKEKILLQIARRCNRKIMVDARKMKVLRVLGHGESGVFTEHESESDVHVVGWNVLGETWP 300
           GKEKILL+IA RCNRKIMVDARKM VLRVLGHGESGVFTE ESESDVHVVGWN+LG+TWP
Sbjct: 241 GKEKILLEIASRCNRKIMVDARKMTVLRVLGHGESGVFTELESESDVHVVGWNILGDTWP 300

Query: 301 YFRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNKFSVRTKDSLEIHLVPYSEHSNYN 360
           YFRPNFVKMND+MVEKGYSKVVGFVPTGWTYEVKRNKFSVR+KDSLEIHLVPYSEHSNYN
Sbjct: 301 YFRPNFVKMNDIMVEKGYSKVVGFVPTGWTYEVKRNKFSVRSKDSLEIHLVPYSEHSNYN 360

Query: 361 ELREYVKFLKPKRVVPTVGLDVDKLDSKHANKIKKHFAGLVDEMANRKEFLRGFHRGSSE 420
           ELREYVKFL+P+RVVPTVG DVDKLDSKH NKIKKHFAGLVDEMANRKEFL+GFHRGSSE
Sbjct: 361 ELREYVKFLRPRRVVPTVGSDVDKLDSKHTNKIKKHFAGLVDEMANRKEFLKGFHRGSSE 420

Query: 421 MSDEVDDNATKAVNECQDFKEKEISLCMNISVKTEACSSGMGLSSLQEVGSTGLTVSNDE 480
           MSD+V DN  KAVNE +D +EKEIS CM +SVKTEACSSGMG SSL+EVGST L   NDE
Sbjct: 421 MSDDVYDNDAKAVNEFRDLEEKEISSCMKMSVKTEACSSGMGFSSLKEVGSTDL---NDE 480

Query: 481 EQKKIIQELRECLPAWVTQDQMLDLISSSGRNVVDAVSIFYERETEFCEHVTTFTDNLPK 540
           +++KIIQELRECLPAWVTQDQMLDLISSSGRN+VDAVS FYERETEFCEH+TT T+NLPK
Sbjct: 481 DKRKIIQELRECLPAWVTQDQMLDLISSSGRNLVDAVSNFYERETEFCEHITTLTENLPK 540

Query: 541 FQTKVLDDPFTFPTTDMKKKIVLENFDISSSQDSKSSSTRCITKSNASPGKRKRNIQERP 600
           FQ+KVLD PFTF TTD+KKKIVL  F+ISSSQDSK+SS RC+ KSNASPGKRKRNIQE+P
Sbjct: 541 FQSKVLDVPFTFHTTDIKKKIVLGKFEISSSQDSKASSPRCMAKSNASPGKRKRNIQEKP 600

Query: 601 KNKSKNNS----------------------SLQSGGSKQSTITKFFRKASNLSEGAKMES 660
           +N+SK NS                      SLQSGGSKQSTITKFFRKAS+LSEG KMES
Sbjct: 601 QNRSKKNSSLQSSGSKKKSSLQSSGSKKILSLQSGGSKQSTITKFFRKASHLSEGDKMES 660

Query: 661 TVEQSCQFQSSPHDITKNANKIDRFIEIINGDESLKELVASLLVETNGDMNMALDLYYAN 720
           TVEQSCQ QS+ +DI K+ NKIDRFI+IINGDESLK+LV SLLVETNGD+NMALDLYYAN
Sbjct: 661 TVEQSCQDQSTSYDINKDTNKIDRFIQIINGDESLKQLVTSLLVETNGDINMALDLYYAN 720

Query: 721 PQGRPVQNEKGHIFSGNPVQAESNLDECLSTQKENMRENFGLETDLSKLSGSTKKINEPL 775
           PQ R  QNEKGHIFSGNP+Q ESNLDECLSTQKE M ENFGLETD SKLS STKKINEPL
Sbjct: 721 PQERLGQNEKGHIFSGNPIQIESNLDECLSTQKEKMAENFGLETDFSKLSSSTKKINEPL 780

BLAST of Sgr021061 vs. NCBI nr
Match: XP_038878216.1 (DNA ligase 6-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1277.7 bits (3305), Expect = 0.0e+00
Identity = 648/775 (83.61%), Postives = 694/775 (89.55%), Query Frame = 0

Query: 1   MASQSSTPKHPSIDSSLSFVTAIESFSRHSHLPLLPPTLPFRPPPFPSDFPQSKLIPKSR 60
           MAS+SSTP+  +IDSSL FVTAIESFSR  +L L P  LPFRPPPFPSDFPQSKLIPKSR
Sbjct: 34  MASESSTPEPLTIDSSLCFVTAIESFSRQLNLSLPPLKLPFRPPPFPSDFPQSKLIPKSR 93

Query: 61  FVVDAFGYAGDYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLVEVLNVPSPFVQ 120
           FVVDAFG+AG+YS+SYFLSHFHSDHYGGLSP+WCKGIIFCS  TA LLVEVL VP PFVQ
Sbjct: 94  FVVDAFGHAGEYSLSYFLSHFHSDHYGGLSPTWCKGIIFCSHLTACLLVEVLKVPLPFVQ 153

Query: 121 PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVHTGDFRFCDSMRGDPV 180
           PLPLREP  IDGCEVIL+DANHCPGAVQFLFKIP GDGK ERYVHTGDFRFCDSMR DP+
Sbjct: 154 PLPLREPTTIDGCEVILVDANHCPGAVQFLFKIPCGDGKFERYVHTGDFRFCDSMRVDPI 213

Query: 181 LETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASKGSMGDVLFLVATYVI 240
           LETF GSDA+FLDTTYCNPKFIFPSQEESIEYIV++I KIGG SKGSMG+VLFLV+TYVI
Sbjct: 214 LETFTGSDAVFLDTTYCNPKFIFPSQEESIEYIVNIINKIGGGSKGSMGNVLFLVSTYVI 273

Query: 241 GKEKILLQIARRCNRKIMVDARKMKVLRVLGHGESGVFTEHESESDVHVVGWNVLGETWP 300
           GKEKILLQIA RCNRKIMVDARKM VLRVLG+GESGVFTEHESESDVHVVGWNVLGETWP
Sbjct: 274 GKEKILLQIASRCNRKIMVDARKMTVLRVLGYGESGVFTEHESESDVHVVGWNVLGETWP 333

Query: 301 YFRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNKFSVRTKDSLEIHLVPYSEHSNYN 360
           +FRPNFVKM D+MVEKGYSKVVGFVPTGWTYE+K NKFSVRTKDSLEIHLVPYSEHSNYN
Sbjct: 334 FFRPNFVKMKDIMVEKGYSKVVGFVPTGWTYELKHNKFSVRTKDSLEIHLVPYSEHSNYN 393

Query: 361 ELREYVKFLKPKRVVPTVGLDVDKLDSKHANKIKKHFAGLVDEMANRKEFLRGFHRGSSE 420
           ELREYVKFL+PKRVVPTVGLDVDKLDSKHA KIKKHFAGLVDEMANRKEFL+GFHRGSSE
Sbjct: 394 ELREYVKFLRPKRVVPTVGLDVDKLDSKHAGKIKKHFAGLVDEMANRKEFLKGFHRGSSE 453

Query: 421 MSDEVDDNATKAVNECQDFKEKEISLCMNISVKT-EACSSGMGLSSLQEVGSTGLTVSND 480
           MSD+ D+N TKA NE  +F+EKEIS CM ISVK  EACSSGMGLSSLQEVGST L  SND
Sbjct: 454 MSDDADENTTKAENEFWEFEEKEISPCMEISVKAEEACSSGMGLSSLQEVGSTNLFESND 513

Query: 481 EEQKKIIQELRECLPAWVTQDQMLDLISSSGRNVVDAVSIFYERETEFCEHVTTFTDNLP 540
           EEQ KIIQELRECLPAWVTQDQ+LDLI SSGRNVVDAVS FYERETEF EH+TT TDNLP
Sbjct: 514 EEQNKIIQELRECLPAWVTQDQILDLIGSSGRNVVDAVSNFYERETEFYEHITTLTDNLP 573

Query: 541 KFQTKVLDDPFTFPTTDMKKKIVLENFDISSSQDSKSSSTRCITKSNASPGKRKRNIQER 600
           K Q+KVLD PFTFP TD+K K+VL   DISS Q SK SS R  TKS  SPGKRKRN Q++
Sbjct: 574 KHQSKVLDSPFTFPATDIKMKVVLGKVDISSGQASKLSSPRGTTKSKTSPGKRKRNAQDK 633

Query: 601 PKNKSKNNSSLQSGGSKQSTITKFFRKASNLSEGAKMESTVEQSCQFQSSPHDITKNANK 660
           PKNKSK N SLQS GSKQSTITKFFRKAS+LSEG KM+ST EQSCQ QSSP+D+T+  NK
Sbjct: 634 PKNKSKKNPSLQSDGSKQSTITKFFRKASHLSEGPKMKSTEEQSCQDQSSPNDVTEYVNK 693

Query: 661 IDRFIEIINGDESLKELVASLLVETNGDMNMALDLYYANPQGRPVQNEKGHIFSGNPVQA 720
           ID+FI+IINGDESLK LVASLLV+TNGDMNMALDLYYA+PQ R  QNEKGHIFS NPVQ 
Sbjct: 694 IDKFIQIINGDESLKTLVASLLVKTNGDMNMALDLYYADPQERLGQNEKGHIFSENPVQT 753

Query: 721 ESNLDECLSTQKENMRENFGLETDLSKLSGSTKKINEPLLSLPPEKYDPVEHGLY 775
           ESNLDEC+STQKENM EN GL+TDLSKLSGS KKINE  LSLPPE+YDPV+H  +
Sbjct: 754 ESNLDECISTQKENMLENVGLKTDLSKLSGSMKKINETFLSLPPEEYDPVKHACW 808

BLAST of Sgr021061 vs. NCBI nr
Match: XP_038878217.1 (DNA ligase 6-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1277.7 bits (3305), Expect = 0.0e+00
Identity = 648/775 (83.61%), Postives = 694/775 (89.55%), Query Frame = 0

Query: 1   MASQSSTPKHPSIDSSLSFVTAIESFSRHSHLPLLPPTLPFRPPPFPSDFPQSKLIPKSR 60
           MAS+SSTP+  +IDSSL FVTAIESFSR  +L L P  LPFRPPPFPSDFPQSKLIPKSR
Sbjct: 34  MASESSTPEPLTIDSSLCFVTAIESFSRQLNLSLPPLKLPFRPPPFPSDFPQSKLIPKSR 93

Query: 61  FVVDAFGYAGDYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLVEVLNVPSPFVQ 120
           FVVDAFG+AG+YS+SYFLSHFHSDHYGGLSP+WCKGIIFCS  TA LLVEVL VP PFVQ
Sbjct: 94  FVVDAFGHAGEYSLSYFLSHFHSDHYGGLSPTWCKGIIFCSHLTACLLVEVLKVPLPFVQ 153

Query: 121 PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVHTGDFRFCDSMRGDPV 180
           PLPLREP  IDGCEVIL+DANHCPGAVQFLFKIP GDGK ERYVHTGDFRFCDSMR DP+
Sbjct: 154 PLPLREPTTIDGCEVILVDANHCPGAVQFLFKIPCGDGKFERYVHTGDFRFCDSMRVDPI 213

Query: 181 LETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASKGSMGDVLFLVATYVI 240
           LETF GSDA+FLDTTYCNPKFIFPSQEESIEYIV++I KIGG SKGSMG+VLFLV+TYVI
Sbjct: 214 LETFTGSDAVFLDTTYCNPKFIFPSQEESIEYIVNIINKIGGGSKGSMGNVLFLVSTYVI 273

Query: 241 GKEKILLQIARRCNRKIMVDARKMKVLRVLGHGESGVFTEHESESDVHVVGWNVLGETWP 300
           GKEKILLQIA RCNRKIMVDARKM VLRVLG+GESGVFTEHESESDVHVVGWNVLGETWP
Sbjct: 274 GKEKILLQIASRCNRKIMVDARKMTVLRVLGYGESGVFTEHESESDVHVVGWNVLGETWP 333

Query: 301 YFRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNKFSVRTKDSLEIHLVPYSEHSNYN 360
           +FRPNFVKM D+MVEKGYSKVVGFVPTGWTYE+K NKFSVRTKDSLEIHLVPYSEHSNYN
Sbjct: 334 FFRPNFVKMKDIMVEKGYSKVVGFVPTGWTYELKHNKFSVRTKDSLEIHLVPYSEHSNYN 393

Query: 361 ELREYVKFLKPKRVVPTVGLDVDKLDSKHANKIKKHFAGLVDEMANRKEFLRGFHRGSSE 420
           ELREYVKFL+PKRVVPTVGLDVDKLDSKHA KIKKHFAGLVDEMANRKEFL+GFHRGSSE
Sbjct: 394 ELREYVKFLRPKRVVPTVGLDVDKLDSKHAGKIKKHFAGLVDEMANRKEFLKGFHRGSSE 453

Query: 421 MSDEVDDNATKAVNECQDFKEKEISLCMNISVKT-EACSSGMGLSSLQEVGSTGLTVSND 480
           MSD+ D+N TKA NE  +F+EKEIS CM ISVK  EACSSGMGLSSLQEVGST L  SND
Sbjct: 454 MSDDADENTTKAENEFWEFEEKEISPCMEISVKAEEACSSGMGLSSLQEVGSTNLFESND 513

Query: 481 EEQKKIIQELRECLPAWVTQDQMLDLISSSGRNVVDAVSIFYERETEFCEHVTTFTDNLP 540
           EEQ KIIQELRECLPAWVTQDQ+LDLI SSGRNVVDAVS FYERETEF EH+TT TDNLP
Sbjct: 514 EEQNKIIQELRECLPAWVTQDQILDLIGSSGRNVVDAVSNFYERETEFYEHITTLTDNLP 573

Query: 541 KFQTKVLDDPFTFPTTDMKKKIVLENFDISSSQDSKSSSTRCITKSNASPGKRKRNIQER 600
           K Q+KVLD PFTFP TD+K K+VL   DISS Q SK SS R  TKS  SPGKRKRN Q++
Sbjct: 574 KHQSKVLDSPFTFPATDIKMKVVLGKVDISSGQASKLSSPRGTTKSKTSPGKRKRNAQDK 633

Query: 601 PKNKSKNNSSLQSGGSKQSTITKFFRKASNLSEGAKMESTVEQSCQFQSSPHDITKNANK 660
           PKNKSK N SLQS GSKQSTITKFFRKAS+LSEG KM+ST EQSCQ QSSP+D+T+  NK
Sbjct: 634 PKNKSKKNPSLQSDGSKQSTITKFFRKASHLSEGPKMKSTEEQSCQDQSSPNDVTEYVNK 693

Query: 661 IDRFIEIINGDESLKELVASLLVETNGDMNMALDLYYANPQGRPVQNEKGHIFSGNPVQA 720
           ID+FI+IINGDESLK LVASLLV+TNGDMNMALDLYYA+PQ R  QNEKGHIFS NPVQ 
Sbjct: 694 IDKFIQIINGDESLKTLVASLLVKTNGDMNMALDLYYADPQERLGQNEKGHIFSENPVQT 753

Query: 721 ESNLDECLSTQKENMRENFGLETDLSKLSGSTKKINEPLLSLPPEKYDPVEHGLY 775
           ESNLDEC+STQKENM EN GL+TDLSKLSGS KKINE  LSLPPE+YDPV+H  +
Sbjct: 754 ESNLDECISTQKENMLENVGLKTDLSKLSGSMKKINETFLSLPPEEYDPVKHACW 808

BLAST of Sgr021061 vs. NCBI nr
Match: XP_008444061.1 (PREDICTED: DNA ligase 1 isoform X1 [Cucumis melo])

HSP 1 Score: 1220.3 bits (3156), Expect = 0.0e+00
Identity = 619/774 (79.97%), Postives = 676/774 (87.34%), Query Frame = 0

Query: 1   MASQSSTPKHPSIDSSLSFVTAIESFSRHSHLPLLPPTLPFRPPPFPSDFPQSKLIPKSR 60
           MAS+SSTPK  +IDSSL FVTAIESFSR  +L L P  LPFRPPPFPSDFPQSKLIP SR
Sbjct: 1   MASESSTPKPLAIDSSLCFVTAIESFSRQLNLSLPPLKLPFRPPPFPSDFPQSKLIPNSR 60

Query: 61  FVVDAFGYAGDYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLVEVLNVPSPFVQ 120
           FVVDAFG+AGDYS+SYFLSHFHSDHYGGLSP+WCKGIIFCS  TA LL+EVL VP PFV 
Sbjct: 61  FVVDAFGHAGDYSLSYFLSHFHSDHYGGLSPTWCKGIIFCSHLTARLLLEVLKVPLPFVH 120

Query: 121 PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVHTGDFRFCDSMRGDPV 180
           PLPLR+P  IDGCEVIL+DANHCPGAVQFLFKIP GDGK ERYVHTGDFRF DSMR DPV
Sbjct: 121 PLPLRQPTTIDGCEVILVDANHCPGAVQFLFKIPCGDGKFERYVHTGDFRFFDSMRVDPV 180

Query: 181 LETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASKGSMGDVLFLVATYVI 240
           LE FIGSDA+FLDTTYCNPKF FPSQEESIEYIV++IQ+ GGASKGSMG+VLFLVATYVI
Sbjct: 181 LERFIGSDAVFLDTTYCNPKFSFPSQEESIEYIVNIIQQTGGASKGSMGNVLFLVATYVI 240

Query: 241 GKEKILLQIARRCNRKIMVDARKMKVLRVLGHGESGVFTEHESESDVHVVGWNVLGETWP 300
           GKEKILLQIA RCNRKIMVDARKM VLRVLG+GESGVFTEHESESDVHVVGWNVLGETWP
Sbjct: 241 GKEKILLQIASRCNRKIMVDARKMTVLRVLGYGESGVFTEHESESDVHVVGWNVLGETWP 300

Query: 301 YFRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNKFSVRTKDSLEIHLVPYSEHSNYN 360
           +FRPNFV++ D+MVEKGYSKVVGFVPTGWTYE+K NKFSVR+KDSLEIHLVPYSEHSNYN
Sbjct: 301 FFRPNFVRIKDIMVEKGYSKVVGFVPTGWTYELKHNKFSVRSKDSLEIHLVPYSEHSNYN 360

Query: 361 ELREYVKFLKPKRVVPTVGLDVDKLDSKHANKIKKHFAGLVDEMANRKEFLRGFHRGSSE 420
           ELREYVKFL+PKRVVPTVGLDVDKLDSKHA+KIKKHFAGLVDEMANRKEFL+GFHRGS E
Sbjct: 361 ELREYVKFLRPKRVVPTVGLDVDKLDSKHADKIKKHFAGLVDEMANRKEFLKGFHRGSGE 420

Query: 421 MSDEVDDNATKAVNECQDFKEKEISLCMNISVKTEACSSGMGLSSLQEVGSTGLTVSNDE 480
           MSD+VD+NATKA  E Q+F+EKEIS CMNISVKT+A +SGMGLSSLQEVG T L  SND 
Sbjct: 421 MSDDVDENATKAETEFQEFEEKEISSCMNISVKTDASNSGMGLSSLQEVGCTNLFESNDG 480

Query: 481 EQKKIIQELRECLPAWVTQDQMLDLISSSGRNVVDAVSIFYERETEFCEHVTTFTDNLPK 540
           EQ+KIIQELRECLPAWVTQDQ+LDLISSSGRNVVDAVS FYERETEF EHV+T TD  PK
Sbjct: 481 EQQKIIQELRECLPAWVTQDQILDLISSSGRNVVDAVSNFYERETEFYEHVSTLTDICPK 540

Query: 541 FQTKVLDDPFTFPTTDMKKKIVLENFDISSSQDSKSSSTRCITKSNASPGKRKRNIQERP 600
           + +K+ D PFTFP TD KK ++L   D SS   SKSSS R   K+  SPGKRKRN Q++P
Sbjct: 541 YPSKISDSPFTFPATDNKKVVILGKVDTSSGHASKSSSPRGTAKTKTSPGKRKRNFQDKP 600

Query: 601 KNKSKNNSSLQSGGSKQSTITKFFRKASNLSEGAKMESTVEQSCQFQSSPHDITKNANKI 660
           KNKS+ N SL SGGSKQSTITKFFRKA +LS+ +KMES  EQ+CQ QSSP+DIT+  NK+
Sbjct: 601 KNKSRKNLSLHSGGSKQSTITKFFRKAPHLSDCSKMESIEEQNCQDQSSPNDITEYVNKM 660

Query: 661 DRFIEIINGDESLKELVASLLVETNGDMNMALDLYYANPQGRPVQNEKGHIFSGNPVQAE 720
           D FI+IINGDESLK LV SLLV+TNGDMNMALDLYY NPQ R  QNEKGHIFSGNP+Q E
Sbjct: 661 DEFIQIINGDESLKTLVESLLVKTNGDMNMALDLYYTNPQERLGQNEKGHIFSGNPIQTE 720

Query: 721 SNLDECLSTQKENMRENFGLETDLSKLSGSTKKINEPLLSLPPEKYDPVEHGLY 775
            N DECLSTQK+NM EN GLETDLS      KKINE  +SLPPE YDPV++  +
Sbjct: 721 RNSDECLSTQKKNMLENIGLETDLS------KKINETFVSLPPEDYDPVKNACW 768

BLAST of Sgr021061 vs. ExPASy Swiss-Prot
Match: F4HPZ9 (DNA ligase 6 OS=Arabidopsis thaliana OX=3702 GN=LIG6 PE=2 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 7.0e-187
Identity = 373/779 (47.88%), Postives = 486/779 (62.39%), Query Frame = 0

Query: 3   SQSSTPKHPSIDSSLSFVTAIESFSRHSHLPLLPPTLPFRPPPFPSDFPQSKLIPKSRFV 62
           S S   +  +++++  + +AI S S     P  P   P    P     P SK IP + F+
Sbjct: 15  SNSDNSETLNLNTTKLYSSAISSIS-----PQFPSPKPTSSCP---SIPNSKRIPNTNFI 74

Query: 63  VDAFGYA-GDYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLVEVLNVPSPFVQP 122
           VD F       SV++FLSHFHSDHY GLS SW KGII+CS  TA L+ E+L VPS FV  
Sbjct: 75  VDLFRLPHQSSSVAFFLSHFHSDHYSGLSSSWSKGIIYCSHKTARLVAEILQVPSQFVFA 134

Query: 123 LPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVHTGDFRFCDSMRGDPVL 182
           LP+ + V IDG EV+LI+ANHCPGAVQFLFK+       E+YVHTGDFRFCD MR DP L
Sbjct: 135 LPMNQMVKIDGSEVVLIEANHCPGAVQFLFKVKLESSGFEKYVHTGDFRFCDEMRFDPFL 194

Query: 183 ETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASKGSMGDVLFLVATYVIG 242
             F+G D +FLDTTYCNPKF+FPSQEES+ Y+V VI KI      S   VLFLVATYV+G
Sbjct: 195 NGFVGCDGVFLDTTYCNPKFVFPSQEESVGYVVSVIDKI------SEEKVLFLVATYVVG 254

Query: 243 KEKILLQIARRCNRKIMVDARKMKVLRVLGHGESGVFTEHESESDVHVVGWNVLGETWPY 302
           KEKIL++IARRC RKI+VDARKM +L VLG GE G+FTE E+ESDVHVVGWNVLGETWPY
Sbjct: 255 KEKILVEIARRCKRKIVVDARKMSMLSVLGCGEEGMFTEDENESDVHVVGWNVLGETWPY 314

Query: 303 FRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNKFSVRTKDSLEIHLVPYSEHSNYNE 362
           FRPNFVKMN++MVEKGY KVVGFVPTGWTYEVKRNKF+VR KDS+EIHLVPYSEHSNY+E
Sbjct: 315 FRPNFVKMNEIMVEKGYDKVVGFVPTGWTYEVKRNKFAVRFKDSMEIHLVPYSEHSNYDE 374

Query: 363 LREYVKFLKPKRVVPTVGLDVDKLDSKHANKIKKHFAGLVDEMANRKEFLRGFHRGSSEM 422
           LRE++KFLKPKRV+PTVG+D++K D K  NK++KHF+GLVDEMAN+K+FL GF+R S + 
Sbjct: 375 LREFIKFLKPKRVIPTVGVDIEKFDCKEVNKMQKHFSGLVDEMANKKDFLLGFYRQSYQK 434

Query: 423 SDEVDDNATKAVNECQDFKEKEISLCMNISVKTEACSSGMGLSSLQEVGSTGLTVSND-- 482
           +++ D +      E  + +EK             AC  G      + V S+   + +D  
Sbjct: 435 NEKSDVDVVSHSAEVYEEEEK------------NACEDGG-----ENVPSSRGPILHDTT 494

Query: 483 -EEQKKIIQELRECLPAWVTQDQMLDLISSSGRNVVDAVSIFYERETEFCEHVTTFTDNL 542
                +++ +LR+ LPAWVT++QMLDLI     N VD VS FYE E E  +  +  T +L
Sbjct: 495 PSSDSRLLIKLRDSLPAWVTEEQMLDLIKKHAGNPVDIVSNFYEYEAELYKQASLPTPSL 554

Query: 543 PKFQTKVLDDPFTFPTTDMKKKIVLENFDISSSQDSKSSSTRCITKSNASPGKRKRNIQE 602
              Q  + DD  T    +  K I  +   I    D        +TK   SPGKR ++   
Sbjct: 555 NN-QAVLFDDDVTDLQPNPVKGICPDVQAIQKGFDLPRKMN--LTKGTISPGKRGKSSGS 614

Query: 603 RPKNKSKNNSSLQSGGSKQSTITKFFRKA-SNLSEGAKMESTVEQSCQFQSSPHDITKNA 662
           +   K+K +   +  G  Q T+ KFF K     S    + S  E+    +   H     A
Sbjct: 615 KSNKKAKKDPKSKPVGPGQPTLFKFFNKVLDGGSNSVSVGSETEECNTDKKMVHIDASEA 674

Query: 663 NK--IDRFIEIINGDESLKELVASLLVETNGDMNMALDLYYANPQGRPVQNEKGHIFSGN 722
            K   D+FI+I+NG ESL++  AS++ E  GD++ AL++YY+ P+  P  +      S  
Sbjct: 675 YKEVTDQFIDIVNGSESLRDYAASIIDEAKGDISRALNIYYSKPREIPGDHAGERGLSSK 734

Query: 723 PVQAESNLDECLSTQKENMRENFGLETDLSKLSGSTKKINEPLLSLPPEKYDPVEHGLY 775
            +Q     + C S + +   EN G   ++   + + + +++  +SLPPEKY P EH  +
Sbjct: 735 TIQYPKCSEACSSQEDKKASENSGHAVNICVQTSAEESVDKNYVSLPPEKYQPKEHACW 759

BLAST of Sgr021061 vs. ExPASy Swiss-Prot
Match: Q6PJP8 (DNA cross-link repair 1A protein OS=Homo sapiens OX=9606 GN=DCLRE1A PE=1 SV=3)

HSP 1 Score: 210.7 bits (535), Expect = 5.9e-53
Identity = 125/341 (36.66%), Postives = 193/341 (56.60%), Query Frame = 0

Query: 51   PQSKLIPKSRFVVDAFGY-AGDYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLV 110
            P  K IP + F VDAF Y   +   +YFL+HFHSDHY GLS  +    ++CS+ T  LL 
Sbjct: 702  PFYKKIPGTGFTVDAFQYGVVEGCTAYFLTHFHSDHYAGLSKHFTFP-VYCSEITGNLLK 761

Query: 111  EVLNVPSPFVQPLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVHTGDF 170
              L+V   ++ PLPL     ++G +V+L+DANHCPGAV  LF +P+G   L    HTGDF
Sbjct: 762  NKLHVQEQYIHPLPLDTECIVNGVKVVLLDANHCPGAVMILFYLPNGTVIL----HTGDF 821

Query: 171  RFCDSMRGDPVLETFIGSD----AIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASK 230
                  R DP +E  + +D     ++LDTTYC+P++ FPSQ+E I + ++   +    + 
Sbjct: 822  ------RADPSMERSLLADQKVHMLYLDTTYCSPEYTFPSQQEVIRFAINTAFEAVTLNP 881

Query: 231  GSMGDVLFLVATYVIGKEKILLQIARRCNRKIMVDARKMKVLRVLGHGE-SGVFTEHESE 290
                  L +  TY IGKEK+ L IA     K+ +   K K L+ L   E + + T     
Sbjct: 882  ----HALVVCGTYSIGKEKVFLAIADVLGSKVGMSQEKYKTLQCLNIPEINSLITTDMCS 941

Query: 291  SDVHVVGWNVLGETWPYFRPNFVKMNDMMVEKG--YSKVVGFVPTGWTYEVKRNKFS--- 350
            S VH++         P  + NF  +   + + G  Y++++ F PTGWT+  K  + +   
Sbjct: 942  SLVHLL---------PMMQINFKGLQSHLKKCGGKYNQILAFRPTGWTHSNKFTRIADVI 1001

Query: 351  VRTKDSLEIHLVPYSEHSNYNELREYVKFLKPKRVVPTVGL 381
             +TK ++ I+ +PYSEHS+Y E++ +V++LKP++++PTV +
Sbjct: 1002 PQTKGNISIYGIPYSEHSSYLEMKRFVQWLKPQKIIPTVNV 1018

BLAST of Sgr021061 vs. ExPASy Swiss-Prot
Match: Q86KS1 (DNA cross-link repair 1 protein OS=Dictyostelium discoideum OX=44689 GN=dclre1 PE=3 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 2.3e-52
Identity = 123/359 (34.26%), Postives = 193/359 (53.76%), Query Frame = 0

Query: 51  PQSKLIPKSRFVVDAFGYAGDYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLVE 110
           P  K+I  + F+VD F Y  +    YFL+HFHSDHY G++ +W  G I+C++ T  L+  
Sbjct: 261 PSFKVIDGTNFLVDGFQYKSEDFTHYFLTHFHSDHYVGITKTWSFGNIYCTEETGKLVSH 320

Query: 111 VLNVPSPFVQPLPLREPVAIDGCEVILIDANHCPGAVQFLFKIP--SGDGKL---ERYVH 170
            L V   ++      + + I G +V  +D+NHCPG+   LF IP  + DG++   E  +H
Sbjct: 321 KLGVDQRYIVKCEWNKLIEIQGVKVAFLDSNHCPGSALILFIIPLRNKDGEIIGEESILH 380

Query: 171 TGDFRFCDSMRGDPVLETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASK 230
           TGDFR+  SM   P+L+    S  ++LD TYC+P+++FP Q E I+ +  +++      K
Sbjct: 381 TGDFRYNQSMNNYPLLKGRTIS-KLYLDNTYCDPQYVFPPQPEIIKQVASIVR------K 440

Query: 231 GSMGDVLFLVATYVIGKEKILLQIARRCNRKIMVDARKMKVLRVLGHG-ESGVFTEHESE 290
            + G+ LFL  TYVIGKE+ILL+IA++  + + V   K  +L  L    +   FT +E  
Sbjct: 441 ENDGETLFLFGTYVIGKERILLEIAKQEGKPVHVSNEKYAILCCLSTCLDINKFTTNELI 500

Query: 291 SDVHVVGWNVLGETWPYFRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNKFSVRTKD 350
           +    V  ++L         N + + D    K Y +V+GF PTGWT   K    +   + 
Sbjct: 501 TPFRAVTMSMLS------YHNMLSLLDSSNNK-YKRVIGFRPTGWTQAKK--SITYLNRG 560

Query: 351 SLEIHLVPYSEHSNYNELREYVKFLKPKRVVPTVGLDVDKLDSKHANKIKKHFAGLVDE 404
               + V YSEHS++NELR+ +   +P +++PTV  D           I  HF+ L ++
Sbjct: 561 PTTFYSVAYSEHSSFNELRDCIDHFRPTQIIPTVDCD----SPIKVKNILAHFSDLYEK 599

BLAST of Sgr021061 vs. ExPASy Swiss-Prot
Match: Q9JIC3 (DNA cross-link repair 1A protein OS=Mus musculus OX=10090 GN=Dclre1a PE=1 SV=2)

HSP 1 Score: 208.0 bits (528), Expect = 3.9e-52
Identity = 122/354 (34.46%), Postives = 196/354 (55.37%), Query Frame = 0

Query: 51   PQSKLIPKSRFVVDAFGYAG-DYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLV 110
            P  K IP + F VDAF Y   +   +YFL+HFHSDHY GLS  + +  ++CS+ T  LL 
Sbjct: 688  PFYKRIPGTGFTVDAFQYGEIEGCTAYFLTHFHSDHYAGLSKDFTRP-VYCSEITGNLLK 747

Query: 111  EVLNVPSPFVQPLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVHTGDF 170
            + L V   +++ LP+     +D  +V+L+DANHCPGA   LF++P+G       +HTGDF
Sbjct: 748  KKLRVQEQYIRQLPMDTECVVDSVKVVLLDANHCPGATMILFQLPNG----AVILHTGDF 807

Query: 171  RFCDSMRGDPVLETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASKGSMG 230
            R   SM    +    +    +FLDTTYC+P++ FPSQ+E I++ ++   +    +  +  
Sbjct: 808  RADPSMERSRLAGRKV--HTLFLDTTYCSPEYTFPSQQEVIQFAINTAFEAVTLNPRA-- 867

Query: 231  DVLFLVATYVIGKEKILLQIARRCNRKIMVDARKMKVLRVLGHGE-SGVFTEHESESDVH 290
              L +  TY IGKEK+ L IA     K+ +   K K L+ L   E S + T    +S VH
Sbjct: 868  --LVVCGTYCIGKEKVFLAIADVLGSKVGMSQEKYKTLQCLNIPEVSSLITTDMCDSLVH 927

Query: 291  VVGWNVLGETWPYFRPNFVKMNDMMVEKG--YSKVVGFVPTGWTYE---VKRNKFSVRTK 350
            ++         P  + NF  +   + + G  Y +++ F PTGWT+            +T+
Sbjct: 928  LL---------PMMQINFKGLQSHLKKCGGKYDQILAFRPTGWTHSNNITSTADIIPQTR 987

Query: 351  DSLEIHLVPYSEHSNYNELREYVKFLKPKRVVPTVGLDVDKLDSKHANKIKKHF 398
             ++ I+ +PYSEHS+Y E++ +V++LKP++++PTV +       +  N ++K+F
Sbjct: 988  GNISIYGIPYSEHSSYLEMKRFVQWLKPQKIIPTVNVG----SFRSRNTMEKYF 1017

BLAST of Sgr021061 vs. ExPASy Swiss-Prot
Match: Q5QJC4 (DNA cross-link repair 1A protein OS=Gallus gallus OX=9031 GN=DCLRE1A PE=1 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 9.5e-51
Identity = 126/367 (34.33%), Postives = 199/367 (54.22%), Query Frame = 0

Query: 51  PQSKLIPKSRFVVDAFGYAG-DYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLV 110
           P  K IP + F VDAF Y   +   +YFL+HFHSDHY GL+ ++    ++C+  T  L+ 
Sbjct: 631 PFYKKIPGTGFTVDAFQYGEIEGCTAYFLTHFHSDHYCGLTKNFVFP-LYCNKITGNLVK 690

Query: 111 EVLNVPSPFVQPLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVHTGDF 170
             L V   ++  LP+     ++G +V+L+DANHCPGA   LF +PSG   L    HTGDF
Sbjct: 691 SKLRVKEQYINVLPMDTECIVNGIKVLLLDANHCPGATMILFYLPSGTAIL----HTGDF 750

Query: 171 RFCDSMRGDPVLETFIGS--DAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASKGS 230
           R   SM   P L   IG     ++LDTTYC+P++ FPSQ+E I++ V+   ++   +  +
Sbjct: 751 RADPSMERYPAL---IGQKIHTLYLDTTYCSPEYTFPSQQEVIQFAVNTAFEMVTLNPRT 810

Query: 231 MGDVLFLVATYVIGKEKILLQIARRCNRKIMVDARKMKVLRVLGHGESGVFTEHESESDV 290
               L +  TY IGKEK+ L IA     K  +   K K L+ L         E  + + +
Sbjct: 811 ----LVVCGTYSIGKEKVFLAIAEVLGSKASMSRDKYKTLQCL---------ESAAVNSL 870

Query: 291 HVVGWN-VLGETWPYFRPNFVKMNDMM--VEKGYSKVVGFVPTGWTYE---VKRNKFSVR 350
             + W+  L    P  + NF  + D +    + + +V+ F PTGWTY    +       +
Sbjct: 871 ITMNWDGTLLHILPMMQINFKGLQDHLNKFSENFDQVLAFKPTGWTYSDSCLSVMDIKPQ 930

Query: 351 TKDSLEIHLVPYSEHSNYNELREYVKFLKPKRVVPTVGLDVDKLDSKHANKIKKHFAGLV 409
           T+ ++ I+ +PYSEHS+Y E++ +V++LKP++++PTV +     D +  + ++KHF   +
Sbjct: 931 TRGNITIYGIPYSEHSSYLEMKRFVQWLKPQKIIPTVNVG----DWRARSLMEKHFRDWM 972

BLAST of Sgr021061 vs. ExPASy TrEMBL
Match: A0A6J1DXE4 (DNA ligase OS=Momordica charantia OX=3673 GN=LOC111024977 PE=3 SV=1)

HSP 1 Score: 1325.1 bits (3428), Expect = 0.0e+00
Identity = 673/796 (84.55%), Postives = 718/796 (90.20%), Query Frame = 0

Query: 1   MASQSSTPKHPSIDSSLSFVTAIESFSRHSHLPLLPPTLPFRPPPFPSDFPQSKLIPKSR 60
           MAS S T  H +IDSSLSFVTAIE FSRHSHLPLLPP LPFRPPPFPSDFPQSKLIPKSR
Sbjct: 1   MASDSPTHIHQTIDSSLSFVTAIELFSRHSHLPLLPPPLPFRPPPFPSDFPQSKLIPKSR 60

Query: 61  FVVDAFGYAGDYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLVEVLNVPSPFVQ 120
           FVVDAFG AGDYSVSYFLSHFHSDHYGGLS SWCKGIIFCSDTTA LL+EVL VPSPFV+
Sbjct: 61  FVVDAFGLAGDYSVSYFLSHFHSDHYGGLSISWCKGIIFCSDTTARLLLEVLKVPSPFVR 120

Query: 121 PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVHTGDFRFCDSMRGDPV 180
           PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIP GDGKLERYVHTGDFRFCDSMR DP+
Sbjct: 121 PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPGGDGKLERYVHTGDFRFCDSMRVDPI 180

Query: 181 LETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASKGSMGDVLFLVATYVI 240
           LE FIGSDAIFLDTTYCNPKFIFP+QEESIEYIV++IQKIGGASKGSMG+VLFLVATYVI
Sbjct: 181 LEEFIGSDAIFLDTTYCNPKFIFPTQEESIEYIVNIIQKIGGASKGSMGNVLFLVATYVI 240

Query: 241 GKEKILLQIARRCNRKIMVDARKMKVLRVLGHGESGVFTEHESESDVHVVGWNVLGETWP 300
           GKEKILL+IA RCNRKIMVDARKM VLRVLGHGESGVFTE ESESDVHVVGWN+LG+TWP
Sbjct: 241 GKEKILLEIASRCNRKIMVDARKMTVLRVLGHGESGVFTELESESDVHVVGWNILGDTWP 300

Query: 301 YFRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNKFSVRTKDSLEIHLVPYSEHSNYN 360
           YFRPNFVKMND+MVEKGYSKVVGFVPTGWTYEVKRNKFSVR+KDSLEIHLVPYSEHSNYN
Sbjct: 301 YFRPNFVKMNDIMVEKGYSKVVGFVPTGWTYEVKRNKFSVRSKDSLEIHLVPYSEHSNYN 360

Query: 361 ELREYVKFLKPKRVVPTVGLDVDKLDSKHANKIKKHFAGLVDEMANRKEFLRGFHRGSSE 420
           ELREYVKFL+P+RVVPTVG DVDKLDSKH NKIKKHFAGLVDEMANRKEFL+GFHRGSSE
Sbjct: 361 ELREYVKFLRPRRVVPTVGSDVDKLDSKHTNKIKKHFAGLVDEMANRKEFLKGFHRGSSE 420

Query: 421 MSDEVDDNATKAVNECQDFKEKEISLCMNISVKTEACSSGMGLSSLQEVGSTGLTVSNDE 480
           MSD+V DN  KAVNE +D +EKEIS CM +SVKTEACSSGMG SSL+EVGST L   NDE
Sbjct: 421 MSDDVYDNDAKAVNEFRDLEEKEISSCMKMSVKTEACSSGMGFSSLKEVGSTDL---NDE 480

Query: 481 EQKKIIQELRECLPAWVTQDQMLDLISSSGRNVVDAVSIFYERETEFCEHVTTFTDNLPK 540
           +++KIIQELRECLPAWVTQDQMLDLISSSGRN+VDAVS FYERETEFCEH+TT T+NLPK
Sbjct: 481 DKRKIIQELRECLPAWVTQDQMLDLISSSGRNLVDAVSNFYERETEFCEHITTLTENLPK 540

Query: 541 FQTKVLDDPFTFPTTDMKKKIVLENFDISSSQDSKSSSTRCITKSNASPGKRKRNIQERP 600
           FQ+KVLD PFTF TTD+KKKIVL  F+ISSSQDSK+SS RC+ KSNASPGKRKRNIQE+P
Sbjct: 541 FQSKVLDVPFTFHTTDIKKKIVLGKFEISSSQDSKASSPRCMAKSNASPGKRKRNIQEKP 600

Query: 601 KNKSKNNS----------------------SLQSGGSKQSTITKFFRKASNLSEGAKMES 660
           +N+SK NS                      SLQSGGSKQSTITKFFRKAS+LSEG KMES
Sbjct: 601 QNRSKKNSSLQSSGSKKKSSLQSSGSKKILSLQSGGSKQSTITKFFRKASHLSEGDKMES 660

Query: 661 TVEQSCQFQSSPHDITKNANKIDRFIEIINGDESLKELVASLLVETNGDMNMALDLYYAN 720
           TVEQSCQ QS+ +DI K+ NKIDRFI+IINGDESLK+LV SLLVETNGD+NMALDLYYAN
Sbjct: 661 TVEQSCQDQSTSYDINKDTNKIDRFIQIINGDESLKQLVTSLLVETNGDINMALDLYYAN 720

Query: 721 PQGRPVQNEKGHIFSGNPVQAESNLDECLSTQKENMRENFGLETDLSKLSGSTKKINEPL 775
           PQ R  QNEKGHIFSGNP+Q ESNLDECLSTQKE M ENFGLETD SKLS STKKINEPL
Sbjct: 721 PQERLGQNEKGHIFSGNPIQIESNLDECLSTQKEKMAENFGLETDFSKLSSSTKKINEPL 780

BLAST of Sgr021061 vs. ExPASy TrEMBL
Match: A0A6J1E130 (DNA ligase OS=Momordica charantia OX=3673 GN=LOC111024977 PE=3 SV=1)

HSP 1 Score: 1325.1 bits (3428), Expect = 0.0e+00
Identity = 673/796 (84.55%), Postives = 718/796 (90.20%), Query Frame = 0

Query: 1   MASQSSTPKHPSIDSSLSFVTAIESFSRHSHLPLLPPTLPFRPPPFPSDFPQSKLIPKSR 60
           MAS S T  H +IDSSLSFVTAIE FSRHSHLPLLPP LPFRPPPFPSDFPQSKLIPKSR
Sbjct: 1   MASDSPTHIHQTIDSSLSFVTAIELFSRHSHLPLLPPPLPFRPPPFPSDFPQSKLIPKSR 60

Query: 61  FVVDAFGYAGDYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLVEVLNVPSPFVQ 120
           FVVDAFG AGDYSVSYFLSHFHSDHYGGLS SWCKGIIFCSDTTA LL+EVL VPSPFV+
Sbjct: 61  FVVDAFGLAGDYSVSYFLSHFHSDHYGGLSISWCKGIIFCSDTTARLLLEVLKVPSPFVR 120

Query: 121 PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVHTGDFRFCDSMRGDPV 180
           PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIP GDGKLERYVHTGDFRFCDSMR DP+
Sbjct: 121 PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPGGDGKLERYVHTGDFRFCDSMRVDPI 180

Query: 181 LETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASKGSMGDVLFLVATYVI 240
           LE FIGSDAIFLDTTYCNPKFIFP+QEESIEYIV++IQKIGGASKGSMG+VLFLVATYVI
Sbjct: 181 LEEFIGSDAIFLDTTYCNPKFIFPTQEESIEYIVNIIQKIGGASKGSMGNVLFLVATYVI 240

Query: 241 GKEKILLQIARRCNRKIMVDARKMKVLRVLGHGESGVFTEHESESDVHVVGWNVLGETWP 300
           GKEKILL+IA RCNRKIMVDARKM VLRVLGHGESGVFTE ESESDVHVVGWN+LG+TWP
Sbjct: 241 GKEKILLEIASRCNRKIMVDARKMTVLRVLGHGESGVFTELESESDVHVVGWNILGDTWP 300

Query: 301 YFRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNKFSVRTKDSLEIHLVPYSEHSNYN 360
           YFRPNFVKMND+MVEKGYSKVVGFVPTGWTYEVKRNKFSVR+KDSLEIHLVPYSEHSNYN
Sbjct: 301 YFRPNFVKMNDIMVEKGYSKVVGFVPTGWTYEVKRNKFSVRSKDSLEIHLVPYSEHSNYN 360

Query: 361 ELREYVKFLKPKRVVPTVGLDVDKLDSKHANKIKKHFAGLVDEMANRKEFLRGFHRGSSE 420
           ELREYVKFL+P+RVVPTVG DVDKLDSKH NKIKKHFAGLVDEMANRKEFL+GFHRGSSE
Sbjct: 361 ELREYVKFLRPRRVVPTVGSDVDKLDSKHTNKIKKHFAGLVDEMANRKEFLKGFHRGSSE 420

Query: 421 MSDEVDDNATKAVNECQDFKEKEISLCMNISVKTEACSSGMGLSSLQEVGSTGLTVSNDE 480
           MSD+V DN  KAVNE +D +EKEIS CM +SVKTEACSSGMG SSL+EVGST L   NDE
Sbjct: 421 MSDDVYDNDAKAVNEFRDLEEKEISSCMKMSVKTEACSSGMGFSSLKEVGSTDL---NDE 480

Query: 481 EQKKIIQELRECLPAWVTQDQMLDLISSSGRNVVDAVSIFYERETEFCEHVTTFTDNLPK 540
           +++KIIQELRECLPAWVTQDQMLDLISSSGRN+VDAVS FYERETEFCEH+TT T+NLPK
Sbjct: 481 DKRKIIQELRECLPAWVTQDQMLDLISSSGRNLVDAVSNFYERETEFCEHITTLTENLPK 540

Query: 541 FQTKVLDDPFTFPTTDMKKKIVLENFDISSSQDSKSSSTRCITKSNASPGKRKRNIQERP 600
           FQ+KVLD PFTF TTD+KKKIVL  F+ISSSQDSK+SS RC+ KSNASPGKRKRNIQE+P
Sbjct: 541 FQSKVLDVPFTFHTTDIKKKIVLGKFEISSSQDSKASSPRCMAKSNASPGKRKRNIQEKP 600

Query: 601 KNKSKNNS----------------------SLQSGGSKQSTITKFFRKASNLSEGAKMES 660
           +N+SK NS                      SLQSGGSKQSTITKFFRKAS+LSEG KMES
Sbjct: 601 QNRSKKNSSLQSSGSKKKSSLQSSGSKKILSLQSGGSKQSTITKFFRKASHLSEGDKMES 660

Query: 661 TVEQSCQFQSSPHDITKNANKIDRFIEIINGDESLKELVASLLVETNGDMNMALDLYYAN 720
           TVEQSCQ QS+ +DI K+ NKIDRFI+IINGDESLK+LV SLLVETNGD+NMALDLYYAN
Sbjct: 661 TVEQSCQDQSTSYDINKDTNKIDRFIQIINGDESLKQLVTSLLVETNGDINMALDLYYAN 720

Query: 721 PQGRPVQNEKGHIFSGNPVQAESNLDECLSTQKENMRENFGLETDLSKLSGSTKKINEPL 775
           PQ R  QNEKGHIFSGNP+Q ESNLDECLSTQKE M ENFGLETD SKLS STKKINEPL
Sbjct: 721 PQERLGQNEKGHIFSGNPIQIESNLDECLSTQKEKMAENFGLETDFSKLSSSTKKINEPL 780

BLAST of Sgr021061 vs. ExPASy TrEMBL
Match: A0A5A7VEZ9 (DNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold548G00160 PE=3 SV=1)

HSP 1 Score: 1220.3 bits (3156), Expect = 0.0e+00
Identity = 619/774 (79.97%), Postives = 676/774 (87.34%), Query Frame = 0

Query: 1   MASQSSTPKHPSIDSSLSFVTAIESFSRHSHLPLLPPTLPFRPPPFPSDFPQSKLIPKSR 60
           MAS+SSTPK  +IDSSL FVTAIESFSR  +L L P  LPFRPPPFPSDFPQSKLIP SR
Sbjct: 1   MASESSTPKPLAIDSSLCFVTAIESFSRQLNLSLPPLKLPFRPPPFPSDFPQSKLIPNSR 60

Query: 61  FVVDAFGYAGDYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLVEVLNVPSPFVQ 120
           FVVDAFG+AGDYS+SYFLSHFHSDHYGGLSP+WCKGIIFCS  TA LL+EVL VP PFV 
Sbjct: 61  FVVDAFGHAGDYSLSYFLSHFHSDHYGGLSPTWCKGIIFCSHLTARLLLEVLKVPLPFVH 120

Query: 121 PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVHTGDFRFCDSMRGDPV 180
           PLPLR+P  IDGCEVIL+DANHCPGAVQFLFKIP GDGK ERYVHTGDFRF DSMR DPV
Sbjct: 121 PLPLRQPTTIDGCEVILVDANHCPGAVQFLFKIPCGDGKFERYVHTGDFRFFDSMRVDPV 180

Query: 181 LETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASKGSMGDVLFLVATYVI 240
           LE FIGSDA+FLDTTYCNPKF FPSQEESIEYIV++IQ+ GGASKGSMG+VLFLVATYVI
Sbjct: 181 LERFIGSDAVFLDTTYCNPKFSFPSQEESIEYIVNIIQQTGGASKGSMGNVLFLVATYVI 240

Query: 241 GKEKILLQIARRCNRKIMVDARKMKVLRVLGHGESGVFTEHESESDVHVVGWNVLGETWP 300
           GKEKILLQIA RCNRKIMVDARKM VLRVLG+GESGVFTEHESESDVHVVGWNVLGETWP
Sbjct: 241 GKEKILLQIASRCNRKIMVDARKMTVLRVLGYGESGVFTEHESESDVHVVGWNVLGETWP 300

Query: 301 YFRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNKFSVRTKDSLEIHLVPYSEHSNYN 360
           +FRPNFV++ D+MVEKGYSKVVGFVPTGWTYE+K NKFSVR+KDSLEIHLVPYSEHSNYN
Sbjct: 301 FFRPNFVRIKDIMVEKGYSKVVGFVPTGWTYELKHNKFSVRSKDSLEIHLVPYSEHSNYN 360

Query: 361 ELREYVKFLKPKRVVPTVGLDVDKLDSKHANKIKKHFAGLVDEMANRKEFLRGFHRGSSE 420
           ELREYVKFL+PKRVVPTVGLDVDKLDSKHA+KIKKHFAGLVDEMANRKEFL+GFHRGS E
Sbjct: 361 ELREYVKFLRPKRVVPTVGLDVDKLDSKHADKIKKHFAGLVDEMANRKEFLKGFHRGSGE 420

Query: 421 MSDEVDDNATKAVNECQDFKEKEISLCMNISVKTEACSSGMGLSSLQEVGSTGLTVSNDE 480
           MSD+VD+NATKA  E Q+F+EKEIS CMNISVKT+A +SGMGLSSLQEVG T L  SND 
Sbjct: 421 MSDDVDENATKAETEFQEFEEKEISSCMNISVKTDASNSGMGLSSLQEVGCTNLFESNDG 480

Query: 481 EQKKIIQELRECLPAWVTQDQMLDLISSSGRNVVDAVSIFYERETEFCEHVTTFTDNLPK 540
           EQ+KIIQELRECLPAWVTQDQ+LDLISSSGRNVVDAVS FYERETEF EHV+T TD  PK
Sbjct: 481 EQQKIIQELRECLPAWVTQDQILDLISSSGRNVVDAVSNFYERETEFYEHVSTLTDICPK 540

Query: 541 FQTKVLDDPFTFPTTDMKKKIVLENFDISSSQDSKSSSTRCITKSNASPGKRKRNIQERP 600
           + +K+ D PFTFP TD KK ++L   D SS   SKSSS R   K+  SPGKRKRN Q++P
Sbjct: 541 YPSKISDSPFTFPATDNKKVVILGKVDTSSGHASKSSSPRGTAKTKTSPGKRKRNFQDKP 600

Query: 601 KNKSKNNSSLQSGGSKQSTITKFFRKASNLSEGAKMESTVEQSCQFQSSPHDITKNANKI 660
           KNKS+ N SL SGGSKQSTITKFFRKA +LS+ +KMES  EQ+CQ QSSP+DIT+  NK+
Sbjct: 601 KNKSRKNLSLHSGGSKQSTITKFFRKAPHLSDCSKMESIEEQNCQDQSSPNDITEYVNKM 660

Query: 661 DRFIEIINGDESLKELVASLLVETNGDMNMALDLYYANPQGRPVQNEKGHIFSGNPVQAE 720
           D FI+IINGDESLK LV SLLV+TNGDMNMALDLYY NPQ R  QNEKGHIFSGNP+Q E
Sbjct: 661 DEFIQIINGDESLKTLVESLLVKTNGDMNMALDLYYTNPQERLGQNEKGHIFSGNPIQTE 720

Query: 721 SNLDECLSTQKENMRENFGLETDLSKLSGSTKKINEPLLSLPPEKYDPVEHGLY 775
            N DECLSTQK+NM EN GLETDLS      KKINE  +SLPPE YDPV++  +
Sbjct: 721 RNSDECLSTQKKNMLENIGLETDLS------KKINETFVSLPPEDYDPVKNACW 768

BLAST of Sgr021061 vs. ExPASy TrEMBL
Match: A0A1S3B9K7 (uncharacterized protein LOC103487505 isoform X6 OS=Cucumis melo OX=3656 GN=LOC103487505 PE=3 SV=1)

HSP 1 Score: 1220.3 bits (3156), Expect = 0.0e+00
Identity = 619/774 (79.97%), Postives = 676/774 (87.34%), Query Frame = 0

Query: 1   MASQSSTPKHPSIDSSLSFVTAIESFSRHSHLPLLPPTLPFRPPPFPSDFPQSKLIPKSR 60
           MAS+SSTPK  +IDSSL FVTAIESFSR  +L L P  LPFRPPPFPSDFPQSKLIP SR
Sbjct: 1   MASESSTPKPLAIDSSLCFVTAIESFSRQLNLSLPPLKLPFRPPPFPSDFPQSKLIPNSR 60

Query: 61  FVVDAFGYAGDYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLVEVLNVPSPFVQ 120
           FVVDAFG+AGDYS+SYFLSHFHSDHYGGLSP+WCKGIIFCS  TA LL+EVL VP PFV 
Sbjct: 61  FVVDAFGHAGDYSLSYFLSHFHSDHYGGLSPTWCKGIIFCSHLTARLLLEVLKVPLPFVH 120

Query: 121 PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVHTGDFRFCDSMRGDPV 180
           PLPLR+P  IDGCEVIL+DANHCPGAVQFLFKIP GDGK ERYVHTGDFRF DSMR DPV
Sbjct: 121 PLPLRQPTTIDGCEVILVDANHCPGAVQFLFKIPCGDGKFERYVHTGDFRFFDSMRVDPV 180

Query: 181 LETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASKGSMGDVLFLVATYVI 240
           LE FIGSDA+FLDTTYCNPKF FPSQEESIEYIV++IQ+ GGASKGSMG+VLFLVATYVI
Sbjct: 181 LERFIGSDAVFLDTTYCNPKFSFPSQEESIEYIVNIIQQTGGASKGSMGNVLFLVATYVI 240

Query: 241 GKEKILLQIARRCNRKIMVDARKMKVLRVLGHGESGVFTEHESESDVHVVGWNVLGETWP 300
           GKEKILLQIA RCNRKIMVDARKM VLRVLG+GESGVFTEHESESDVHVVGWNVLGETWP
Sbjct: 241 GKEKILLQIASRCNRKIMVDARKMTVLRVLGYGESGVFTEHESESDVHVVGWNVLGETWP 300

Query: 301 YFRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNKFSVRTKDSLEIHLVPYSEHSNYN 360
           +FRPNFV++ D+MVEKGYSKVVGFVPTGWTYE+K NKFSVR+KDSLEIHLVPYSEHSNYN
Sbjct: 301 FFRPNFVRIKDIMVEKGYSKVVGFVPTGWTYELKHNKFSVRSKDSLEIHLVPYSEHSNYN 360

Query: 361 ELREYVKFLKPKRVVPTVGLDVDKLDSKHANKIKKHFAGLVDEMANRKEFLRGFHRGSSE 420
           ELREYVKFL+PKRVVPTVGLDVDKLDSKHA+KIKKHFAGLVDEMANRKEFL+GFHRGS E
Sbjct: 361 ELREYVKFLRPKRVVPTVGLDVDKLDSKHADKIKKHFAGLVDEMANRKEFLKGFHRGSGE 420

Query: 421 MSDEVDDNATKAVNECQDFKEKEISLCMNISVKTEACSSGMGLSSLQEVGSTGLTVSNDE 480
           MSD+VD+NATKA  E Q+F+EKEIS CMNISVKT+A +SGMGLSSLQEVG T L  SND 
Sbjct: 421 MSDDVDENATKAETEFQEFEEKEISSCMNISVKTDASNSGMGLSSLQEVGCTNLFESNDG 480

Query: 481 EQKKIIQELRECLPAWVTQDQMLDLISSSGRNVVDAVSIFYERETEFCEHVTTFTDNLPK 540
           EQ+KIIQELRECLPAWVTQDQ+LDLISSSGRNVVDAVS FYERETEF EHV+T TD  PK
Sbjct: 481 EQQKIIQELRECLPAWVTQDQILDLISSSGRNVVDAVSNFYERETEFYEHVSTLTDICPK 540

Query: 541 FQTKVLDDPFTFPTTDMKKKIVLENFDISSSQDSKSSSTRCITKSNASPGKRKRNIQERP 600
           + +K+ D PFTFP TD KK ++L   D SS   SKSSS R   K+  SPGKRKRN Q++P
Sbjct: 541 YPSKISDSPFTFPATDNKKVVILGKVDTSSGHASKSSSPRGTAKTKTSPGKRKRNFQDKP 600

Query: 601 KNKSKNNSSLQSGGSKQSTITKFFRKASNLSEGAKMESTVEQSCQFQSSPHDITKNANKI 660
           KNKS+ N SL SGGSKQSTITKFFRKA +LS+ +KMES  EQ+CQ QSSP+DIT+  NK+
Sbjct: 601 KNKSRKNLSLHSGGSKQSTITKFFRKAPHLSDCSKMESIEEQNCQDQSSPNDITEYVNKM 660

Query: 661 DRFIEIINGDESLKELVASLLVETNGDMNMALDLYYANPQGRPVQNEKGHIFSGNPVQAE 720
           D FI+IINGDESLK LV SLLV+TNGDMNMALDLYY NPQ R  QNEKGHIFSGNP+Q E
Sbjct: 661 DEFIQIINGDESLKTLVESLLVKTNGDMNMALDLYYTNPQERLGQNEKGHIFSGNPIQTE 720

Query: 721 SNLDECLSTQKENMRENFGLETDLSKLSGSTKKINEPLLSLPPEKYDPVEHGLY 775
            N DECLSTQK+NM EN GLETDLS      KKINE  +SLPPE YDPV++  +
Sbjct: 721 RNSDECLSTQKKNMLENIGLETDLS------KKINETFVSLPPEDYDPVKNACW 768

BLAST of Sgr021061 vs. ExPASy TrEMBL
Match: A0A1S3BAA0 (uncharacterized protein LOC103487505 isoform X7 OS=Cucumis melo OX=3656 GN=LOC103487505 PE=3 SV=1)

HSP 1 Score: 1220.3 bits (3156), Expect = 0.0e+00
Identity = 619/774 (79.97%), Postives = 676/774 (87.34%), Query Frame = 0

Query: 1   MASQSSTPKHPSIDSSLSFVTAIESFSRHSHLPLLPPTLPFRPPPFPSDFPQSKLIPKSR 60
           MAS+SSTPK  +IDSSL FVTAIESFSR  +L L P  LPFRPPPFPSDFPQSKLIP SR
Sbjct: 1   MASESSTPKPLAIDSSLCFVTAIESFSRQLNLSLPPLKLPFRPPPFPSDFPQSKLIPNSR 60

Query: 61  FVVDAFGYAGDYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLVEVLNVPSPFVQ 120
           FVVDAFG+AGDYS+SYFLSHFHSDHYGGLSP+WCKGIIFCS  TA LL+EVL VP PFV 
Sbjct: 61  FVVDAFGHAGDYSLSYFLSHFHSDHYGGLSPTWCKGIIFCSHLTARLLLEVLKVPLPFVH 120

Query: 121 PLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVHTGDFRFCDSMRGDPV 180
           PLPLR+P  IDGCEVIL+DANHCPGAVQFLFKIP GDGK ERYVHTGDFRF DSMR DPV
Sbjct: 121 PLPLRQPTTIDGCEVILVDANHCPGAVQFLFKIPCGDGKFERYVHTGDFRFFDSMRVDPV 180

Query: 181 LETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASKGSMGDVLFLVATYVI 240
           LE FIGSDA+FLDTTYCNPKF FPSQEESIEYIV++IQ+ GGASKGSMG+VLFLVATYVI
Sbjct: 181 LERFIGSDAVFLDTTYCNPKFSFPSQEESIEYIVNIIQQTGGASKGSMGNVLFLVATYVI 240

Query: 241 GKEKILLQIARRCNRKIMVDARKMKVLRVLGHGESGVFTEHESESDVHVVGWNVLGETWP 300
           GKEKILLQIA RCNRKIMVDARKM VLRVLG+GESGVFTEHESESDVHVVGWNVLGETWP
Sbjct: 241 GKEKILLQIASRCNRKIMVDARKMTVLRVLGYGESGVFTEHESESDVHVVGWNVLGETWP 300

Query: 301 YFRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNKFSVRTKDSLEIHLVPYSEHSNYN 360
           +FRPNFV++ D+MVEKGYSKVVGFVPTGWTYE+K NKFSVR+KDSLEIHLVPYSEHSNYN
Sbjct: 301 FFRPNFVRIKDIMVEKGYSKVVGFVPTGWTYELKHNKFSVRSKDSLEIHLVPYSEHSNYN 360

Query: 361 ELREYVKFLKPKRVVPTVGLDVDKLDSKHANKIKKHFAGLVDEMANRKEFLRGFHRGSSE 420
           ELREYVKFL+PKRVVPTVGLDVDKLDSKHA+KIKKHFAGLVDEMANRKEFL+GFHRGS E
Sbjct: 361 ELREYVKFLRPKRVVPTVGLDVDKLDSKHADKIKKHFAGLVDEMANRKEFLKGFHRGSGE 420

Query: 421 MSDEVDDNATKAVNECQDFKEKEISLCMNISVKTEACSSGMGLSSLQEVGSTGLTVSNDE 480
           MSD+VD+NATKA  E Q+F+EKEIS CMNISVKT+A +SGMGLSSLQEVG T L  SND 
Sbjct: 421 MSDDVDENATKAETEFQEFEEKEISSCMNISVKTDASNSGMGLSSLQEVGCTNLFESNDG 480

Query: 481 EQKKIIQELRECLPAWVTQDQMLDLISSSGRNVVDAVSIFYERETEFCEHVTTFTDNLPK 540
           EQ+KIIQELRECLPAWVTQDQ+LDLISSSGRNVVDAVS FYERETEF EHV+T TD  PK
Sbjct: 481 EQQKIIQELRECLPAWVTQDQILDLISSSGRNVVDAVSNFYERETEFYEHVSTLTDICPK 540

Query: 541 FQTKVLDDPFTFPTTDMKKKIVLENFDISSSQDSKSSSTRCITKSNASPGKRKRNIQERP 600
           + +K+ D PFTFP TD KK ++L   D SS   SKSSS R   K+  SPGKRKRN Q++P
Sbjct: 541 YPSKISDSPFTFPATDNKKVVILGKVDTSSGHASKSSSPRGTAKTKTSPGKRKRNFQDKP 600

Query: 601 KNKSKNNSSLQSGGSKQSTITKFFRKASNLSEGAKMESTVEQSCQFQSSPHDITKNANKI 660
           KNKS+ N SL SGGSKQSTITKFFRKA +LS+ +KMES  EQ+CQ QSSP+DIT+  NK+
Sbjct: 601 KNKSRKNLSLHSGGSKQSTITKFFRKAPHLSDCSKMESIEEQNCQDQSSPNDITEYVNKM 660

Query: 661 DRFIEIINGDESLKELVASLLVETNGDMNMALDLYYANPQGRPVQNEKGHIFSGNPVQAE 720
           D FI+IINGDESLK LV SLLV+TNGDMNMALDLYY NPQ R  QNEKGHIFSGNP+Q E
Sbjct: 661 DEFIQIINGDESLKTLVESLLVKTNGDMNMALDLYYTNPQERLGQNEKGHIFSGNPIQTE 720

Query: 721 SNLDECLSTQKENMRENFGLETDLSKLSGSTKKINEPLLSLPPEKYDPVEHGLY 775
            N DECLSTQK+NM EN GLETDLS      KKINE  +SLPPE YDPV++  +
Sbjct: 721 RNSDECLSTQKKNMLENIGLETDLS------KKINETFVSLPPEDYDPVKNACW 768

BLAST of Sgr021061 vs. TAIR 10
Match: AT1G66730.1 (DNA LIGASE 6 )

HSP 1 Score: 655.6 bits (1690), Expect = 5.0e-188
Identity = 373/779 (47.88%), Postives = 486/779 (62.39%), Query Frame = 0

Query: 3   SQSSTPKHPSIDSSLSFVTAIESFSRHSHLPLLPPTLPFRPPPFPSDFPQSKLIPKSRFV 62
           S S   +  +++++  + +AI S S     P  P   P    P     P SK IP + F+
Sbjct: 15  SNSDNSETLNLNTTKLYSSAISSIS-----PQFPSPKPTSSCP---SIPNSKRIPNTNFI 74

Query: 63  VDAFGYA-GDYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLVEVLNVPSPFVQP 122
           VD F       SV++FLSHFHSDHY GLS SW KGII+CS  TA L+ E+L VPS FV  
Sbjct: 75  VDLFRLPHQSSSVAFFLSHFHSDHYSGLSSSWSKGIIYCSHKTARLVAEILQVPSQFVFA 134

Query: 123 LPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVHTGDFRFCDSMRGDPVL 182
           LP+ + V IDG EV+LI+ANHCPGAVQFLFK+       E+YVHTGDFRFCD MR DP L
Sbjct: 135 LPMNQMVKIDGSEVVLIEANHCPGAVQFLFKVKLESSGFEKYVHTGDFRFCDEMRFDPFL 194

Query: 183 ETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASKGSMGDVLFLVATYVIG 242
             F+G D +FLDTTYCNPKF+FPSQEES+ Y+V VI KI      S   VLFLVATYV+G
Sbjct: 195 NGFVGCDGVFLDTTYCNPKFVFPSQEESVGYVVSVIDKI------SEEKVLFLVATYVVG 254

Query: 243 KEKILLQIARRCNRKIMVDARKMKVLRVLGHGESGVFTEHESESDVHVVGWNVLGETWPY 302
           KEKIL++IARRC RKI+VDARKM +L VLG GE G+FTE E+ESDVHVVGWNVLGETWPY
Sbjct: 255 KEKILVEIARRCKRKIVVDARKMSMLSVLGCGEEGMFTEDENESDVHVVGWNVLGETWPY 314

Query: 303 FRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNKFSVRTKDSLEIHLVPYSEHSNYNE 362
           FRPNFVKMN++MVEKGY KVVGFVPTGWTYEVKRNKF+VR KDS+EIHLVPYSEHSNY+E
Sbjct: 315 FRPNFVKMNEIMVEKGYDKVVGFVPTGWTYEVKRNKFAVRFKDSMEIHLVPYSEHSNYDE 374

Query: 363 LREYVKFLKPKRVVPTVGLDVDKLDSKHANKIKKHFAGLVDEMANRKEFLRGFHRGSSEM 422
           LRE++KFLKPKRV+PTVG+D++K D K  NK++KHF+GLVDEMAN+K+FL GF+R S + 
Sbjct: 375 LREFIKFLKPKRVIPTVGVDIEKFDCKEVNKMQKHFSGLVDEMANKKDFLLGFYRQSYQK 434

Query: 423 SDEVDDNATKAVNECQDFKEKEISLCMNISVKTEACSSGMGLSSLQEVGSTGLTVSND-- 482
           +++ D +      E  + +EK             AC  G      + V S+   + +D  
Sbjct: 435 NEKSDVDVVSHSAEVYEEEEK------------NACEDGG-----ENVPSSRGPILHDTT 494

Query: 483 -EEQKKIIQELRECLPAWVTQDQMLDLISSSGRNVVDAVSIFYERETEFCEHVTTFTDNL 542
                +++ +LR+ LPAWVT++QMLDLI     N VD VS FYE E E  +  +  T +L
Sbjct: 495 PSSDSRLLIKLRDSLPAWVTEEQMLDLIKKHAGNPVDIVSNFYEYEAELYKQASLPTPSL 554

Query: 543 PKFQTKVLDDPFTFPTTDMKKKIVLENFDISSSQDSKSSSTRCITKSNASPGKRKRNIQE 602
              Q  + DD  T    +  K I  +   I    D        +TK   SPGKR ++   
Sbjct: 555 NN-QAVLFDDDVTDLQPNPVKGICPDVQAIQKGFDLPRKMN--LTKGTISPGKRGKSSGS 614

Query: 603 RPKNKSKNNSSLQSGGSKQSTITKFFRKA-SNLSEGAKMESTVEQSCQFQSSPHDITKNA 662
           +   K+K +   +  G  Q T+ KFF K     S    + S  E+    +   H     A
Sbjct: 615 KSNKKAKKDPKSKPVGPGQPTLFKFFNKVLDGGSNSVSVGSETEECNTDKKMVHIDASEA 674

Query: 663 NK--IDRFIEIINGDESLKELVASLLVETNGDMNMALDLYYANPQGRPVQNEKGHIFSGN 722
            K   D+FI+I+NG ESL++  AS++ E  GD++ AL++YY+ P+  P  +      S  
Sbjct: 675 YKEVTDQFIDIVNGSESLRDYAASIIDEAKGDISRALNIYYSKPREIPGDHAGERGLSSK 734

Query: 723 PVQAESNLDECLSTQKENMRENFGLETDLSKLSGSTKKINEPLLSLPPEKYDPVEHGLY 775
            +Q     + C S + +   EN G   ++   + + + +++  +SLPPEKY P EH  +
Sbjct: 735 TIQYPKCSEACSSQEDKKASENSGHAVNICVQTSAEESVDKNYVSLPPEKYQPKEHACW 759

BLAST of Sgr021061 vs. TAIR 10
Match: AT2G45700.1 (sterile alpha motif (SAM) domain-containing protein )

HSP 1 Score: 211.5 bits (537), Expect = 2.5e-54
Identity = 126/337 (37.39%), Postives = 183/337 (54.30%), Query Frame = 0

Query: 51  PQSKLIPKSRFVVDAFGYAGDYSVSYFLSHFHSDHYGGLSPSWCKGIIFCSDTTAGLLVE 110
           P    IP + F VDAF Y       +FL+HFH DHY GL+ S+  G I+CS  TA L+  
Sbjct: 393 PHWNCIPGTPFRVDAFKYLTRDCCHWFLTHFHLDHYQGLTKSFSHGKIYCSLVTAKLVNM 452

Query: 111 VLNVPSPFVQPLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVHTGDFR 170
            + +P   +Q L L + V I G +V   DANHCPG++  LF+  +G   L    HTGDFR
Sbjct: 453 KIGIPWERLQVLDLGQKVNISGIDVTCFDANHCPGSIMILFEPANGKAVL----HTGDFR 512

Query: 171 FCDSMRGDPVLETFIGS--DAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASKGSM 230
           + + M         IGS   ++ LDTTYCNP++ FP QE  I+++V  IQ     ++   
Sbjct: 513 YSEEMS-----NWLIGSHISSLILDTTYCNPQYDFPKQEAVIQFVVEAIQ-----AEAFN 572

Query: 231 GDVLFLVATYVIGKEKILLQIARRCNRKIMVDARKMKVLRVLGHGESGV--FTEHESESD 290
              LFL+ +Y IGKE++ L++AR    KI ++  K+K+L  LG  +  +  FT  E ES 
Sbjct: 573 PKTLFLIGSYTIGKERLFLEVARVLREKIYINPAKLKLLECLGFSKDDIQWFTVKEEESH 632

Query: 291 VHVVGWNVLGETWPYFRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNKFSVR--TKD 350
           +HVV        W       +K         YS +V F PTGWT    + K   R   + 
Sbjct: 633 IHVV------PLWTLASFKRLKHVANRYTNRYSLIVAFSPTGWTSGKTKKKSPGRRLQQG 692

Query: 351 SLEIHLVPYSEHSNYNELREYVKFLKPKRVVPTVGLD 382
           ++  + VPYSEHS++ EL+E+V+ + P+ ++P+V  D
Sbjct: 693 TIIRYEVPYSEHSSFTELKEFVQKVSPEVIIPSVNND 709

BLAST of Sgr021061 vs. TAIR 10
Match: AT3G26680.1 (DNA repair metallo-beta-lactamase family protein )

HSP 1 Score: 202.2 bits (513), Expect = 1.5e-51
Identity = 121/338 (35.80%), Postives = 183/338 (54.14%), Query Frame = 0

Query: 47  PSDFPQSKLIPKSRFVVDAFGYAGDYSVS-YFLSHFHSDHYGGLSPSWCKGIIFCSDTTA 106
           P   P  K +P + F VDAF Y      S YFL+HFH+DHY GL+ +W  G I+CS  T+
Sbjct: 142 PRPCPFYKKLPGTPFTVDAFRYGCVQGCSAYFLTHFHADHYIGLTKAWSHGPIYCSSLTS 201

Query: 107 GLLVEVLNVPSPFVQPLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVH 166
            LL   L+V    + PL L     I+G +V LI+ANHCPGA    F++  G      Y+H
Sbjct: 202 RLLRLSLSVNPSSIHPLELDVEYTINGIKVTLIEANHCPGAALIHFRLLDG----TCYLH 261

Query: 167 TGDFRFCDSMRGDPVLETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASK 226
           TGDFR    M+  P+L        ++LDTTYCNP++ FPS+E+ + Y+V + +       
Sbjct: 262 TGDFRASKQMQTHPLLFN-QRVHVLYLDTTYCNPRYKFPSKEDVLSYVVRITKDF----L 321

Query: 227 GSMGDVLFLVATYVIGKEKILLQIARRCNRKIMVDARKMKVLRVLGHGE-SGVFTEHESE 286
                 L +V +Y IGKE + L IA+    KI  +A + ++L+  G  + S   +     
Sbjct: 322 RKQPKTLIVVGSYSIGKECVYLAIAKALGVKIFANASRRRILQSFGWDDISKNLSTDGKA 381

Query: 287 SDVHVVGWNVLGETWPYFRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNK----FSV 346
           + +HV+  + L       +   +  +  +  + Y  V+ F PTGWTY  K  +       
Sbjct: 382 TCLHVLPMSSL-------KVERLDEHLKIYREQYGAVLAFRPTGWTYSEKIGEHLDLIKP 441

Query: 347 RTKDSLEIHLVPYSEHSNYNELREYVKFLKPKRVVPTV 379
            ++  + I+ VPYSEHS++ ELRE+V+FL+P +++PTV
Sbjct: 442 TSRGKITIYGVPYSEHSSFTELREFVQFLRPDKIIPTV 463

BLAST of Sgr021061 vs. TAIR 10
Match: AT3G26680.2 (DNA repair metallo-beta-lactamase family protein )

HSP 1 Score: 202.2 bits (513), Expect = 1.5e-51
Identity = 121/338 (35.80%), Postives = 183/338 (54.14%), Query Frame = 0

Query: 47  PSDFPQSKLIPKSRFVVDAFGYAGDYSVS-YFLSHFHSDHYGGLSPSWCKGIIFCSDTTA 106
           P   P  K +P + F VDAF Y      S YFL+HFH+DHY GL+ +W  G I+CS  T+
Sbjct: 142 PRPCPFYKKLPGTPFTVDAFRYGCVQGCSAYFLTHFHADHYIGLTKAWSHGPIYCSSLTS 201

Query: 107 GLLVEVLNVPSPFVQPLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVH 166
            LL   L+V    + PL L     I+G +V LI+ANHCPGA    F++  G      Y+H
Sbjct: 202 RLLRLSLSVNPSSIHPLELDVEYTINGIKVTLIEANHCPGAALIHFRLLDG----TCYLH 261

Query: 167 TGDFRFCDSMRGDPVLETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASK 226
           TGDFR    M+  P+L        ++LDTTYCNP++ FPS+E+ + Y+V + +       
Sbjct: 262 TGDFRASKQMQTHPLLFN-QRVHVLYLDTTYCNPRYKFPSKEDVLSYVVRITKDF----L 321

Query: 227 GSMGDVLFLVATYVIGKEKILLQIARRCNRKIMVDARKMKVLRVLGHGE-SGVFTEHESE 286
                 L +V +Y IGKE + L IA+    KI  +A + ++L+  G  + S   +     
Sbjct: 322 RKQPKTLIVVGSYSIGKECVYLAIAKALGVKIFANASRRRILQSFGWDDISKNLSTDGKA 381

Query: 287 SDVHVVGWNVLGETWPYFRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNK----FSV 346
           + +HV+  + L       +   +  +  +  + Y  V+ F PTGWTY  K  +       
Sbjct: 382 TCLHVLPMSSL-------KVERLDEHLKIYREQYGAVLAFRPTGWTYSEKIGEHLDLIKP 441

Query: 347 RTKDSLEIHLVPYSEHSNYNELREYVKFLKPKRVVPTV 379
            ++  + I+ VPYSEHS++ ELRE+V+FL+P +++PTV
Sbjct: 442 TSRGKITIYGVPYSEHSSFTELREFVQFLRPDKIIPTV 463

BLAST of Sgr021061 vs. TAIR 10
Match: AT3G26680.3 (DNA repair metallo-beta-lactamase family protein )

HSP 1 Score: 202.2 bits (513), Expect = 1.5e-51
Identity = 121/338 (35.80%), Postives = 183/338 (54.14%), Query Frame = 0

Query: 47  PSDFPQSKLIPKSRFVVDAFGYAGDYSVS-YFLSHFHSDHYGGLSPSWCKGIIFCSDTTA 106
           P   P  K +P + F VDAF Y      S YFL+HFH+DHY GL+ +W  G I+CS  T+
Sbjct: 142 PRPCPFYKKLPGTPFTVDAFRYGCVQGCSAYFLTHFHADHYIGLTKAWSHGPIYCSSLTS 201

Query: 107 GLLVEVLNVPSPFVQPLPLREPVAIDGCEVILIDANHCPGAVQFLFKIPSGDGKLERYVH 166
            LL   L+V    + PL L     I+G +V LI+ANHCPGA    F++  G      Y+H
Sbjct: 202 RLLRLSLSVNPSSIHPLELDVEYTINGIKVTLIEANHCPGAALIHFRLLDG----TCYLH 261

Query: 167 TGDFRFCDSMRGDPVLETFIGSDAIFLDTTYCNPKFIFPSQEESIEYIVHVIQKIGGASK 226
           TGDFR    M+  P+L        ++LDTTYCNP++ FPS+E+ + Y+V + +       
Sbjct: 262 TGDFRASKQMQTHPLLFN-QRVHVLYLDTTYCNPRYKFPSKEDVLSYVVRITKDF----L 321

Query: 227 GSMGDVLFLVATYVIGKEKILLQIARRCNRKIMVDARKMKVLRVLGHGE-SGVFTEHESE 286
                 L +V +Y IGKE + L IA+    KI  +A + ++L+  G  + S   +     
Sbjct: 322 RKQPKTLIVVGSYSIGKECVYLAIAKALGVKIFANASRRRILQSFGWDDISKNLSTDGKA 381

Query: 287 SDVHVVGWNVLGETWPYFRPNFVKMNDMMVEKGYSKVVGFVPTGWTYEVKRNK----FSV 346
           + +HV+  + L       +   +  +  +  + Y  V+ F PTGWTY  K  +       
Sbjct: 382 TCLHVLPMSSL-------KVERLDEHLKIYREQYGAVLAFRPTGWTYSEKIGEHLDLIKP 441

Query: 347 RTKDSLEIHLVPYSEHSNYNELREYVKFLKPKRVVPTV 379
            ++  + I+ VPYSEHS++ ELRE+V+FL+P +++PTV
Sbjct: 442 TSRGKITIYGVPYSEHSSFTELREFVQFLRPDKIIPTV 463

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158502.10.0e+0084.55DNA ligase 6-like isoform X2 [Momordica charantia][more]
XP_022158501.10.0e+0084.55DNA ligase 6-like isoform X1 [Momordica charantia][more]
XP_038878216.10.0e+0083.61DNA ligase 6-like isoform X1 [Benincasa hispida][more]
XP_038878217.10.0e+0083.61DNA ligase 6-like isoform X2 [Benincasa hispida][more]
XP_008444061.10.0e+0079.97PREDICTED: DNA ligase 1 isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
F4HPZ97.0e-18747.88DNA ligase 6 OS=Arabidopsis thaliana OX=3702 GN=LIG6 PE=2 SV=1[more]
Q6PJP85.9e-5336.66DNA cross-link repair 1A protein OS=Homo sapiens OX=9606 GN=DCLRE1A PE=1 SV=3[more]
Q86KS12.3e-5234.26DNA cross-link repair 1 protein OS=Dictyostelium discoideum OX=44689 GN=dclre1 P... [more]
Q9JIC33.9e-5234.46DNA cross-link repair 1A protein OS=Mus musculus OX=10090 GN=Dclre1a PE=1 SV=2[more]
Q5QJC49.5e-5134.33DNA cross-link repair 1A protein OS=Gallus gallus OX=9031 GN=DCLRE1A PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DXE40.0e+0084.55DNA ligase OS=Momordica charantia OX=3673 GN=LOC111024977 PE=3 SV=1[more]
A0A6J1E1300.0e+0084.55DNA ligase OS=Momordica charantia OX=3673 GN=LOC111024977 PE=3 SV=1[more]
A0A5A7VEZ90.0e+0079.97DNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold548G00160 PE=... [more]
A0A1S3B9K70.0e+0079.97uncharacterized protein LOC103487505 isoform X6 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A1S3BAA00.0e+0079.97uncharacterized protein LOC103487505 isoform X7 OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
AT1G66730.15.0e-18847.88DNA LIGASE 6 [more]
AT2G45700.12.5e-5437.39sterile alpha motif (SAM) domain-containing protein [more]
AT3G26680.11.5e-5135.80DNA repair metallo-beta-lactamase family protein [more]
AT3G26680.21.5e-5135.80DNA repair metallo-beta-lactamase family protein [more]
AT3G26680.31.5e-5135.80DNA repair metallo-beta-lactamase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011084DNA repair metallo-beta-lactamasePFAMPF07522DRMBLcoord: 276..379
e-value: 4.0E-19
score: 68.9
NoneNo IPR availablePFAMPF14555UBA_4coord: 658..700
e-value: 1.1E-4
score: 21.9
NoneNo IPR availableGENE3D3.40.50.12650coord: 202..356
e-value: 2.8E-83
score: 281.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 754..774
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 602..618
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 570..618
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 570..590
NoneNo IPR availablePANTHERPTHR23240DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATEDcoord: 6..770
NoneNo IPR availablePANTHERPTHR23240:SF24DNA LIGASEcoord: 6..770
NoneNo IPR availableCDDcd16273SNM1A-1C-like_MBL-foldcoord: 54..199
e-value: 6.26999E-69
score: 222.03
IPR036866Ribonuclease Z/Hydroxyacylglutathione hydrolase-likeGENE3D3.60.15.10coord: 56..378
e-value: 2.8E-83
score: 281.8
IPR036866Ribonuclease Z/Hydroxyacylglutathione hydrolase-likeSUPERFAMILY56281Metallo-hydrolase/oxidoreductasecoord: 75..379

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr021061.1Sgr021061.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071897 DNA biosynthetic process
biological_process GO:0006266 DNA ligation
biological_process GO:0006310 DNA recombination
biological_process GO:0006281 DNA repair
biological_process GO:0006260 DNA replication
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0003910 DNA ligase (ATP) activity