Sgr020815 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr020815
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	BOI-related E3 ubiquitin-protein ligase 1-like
Location	tig00153574: 178763 .. 180027 (-)
RNA-Seq Expression	Sgr020815
Synteny	Sgr020815

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGTTGAAGCACGGCGTATGAGTCTTCTACCTTCTCAGTTCATCACGAACAGGTGCGTAATACGCTTCTTCTTCTTCTGAAGATGATGATGGTGATGATGATAACAATAATCTCTTCCCTTTTCGTTGATGGGTTTCTCGGGAATGAAGTTTTTCTCAAATGGGTTCTGGTTTTTTTTTTTAGCAGAGATTTCTTGAAACCGAATCAAGGAAGCGTGGATATGATCTGCAACAGCAGCACCCAGATGACGACGGCGGGATTATTGCCTTACGCGGCGGCCAAAGTGCCGCCGGAGACTGTTCTCCCGTTCTACCAATCTTCTGCTTGCGATTTTGGGGGTTCGGGAAAGGCCGACAGTGGGCTCACTTCTAATTTCCCGGCCGCTCCCCGAAAGAGAACCAGACGGTCGGTTTACGATGAACCAGTCGTCGGTCGGGTTTCCGGCTTGTTCGATCAAGAAATCATCTCCTCCCAGATCCAACACCAACAGTCTGAGATCGATCGCTTCATTGCAATTCACGTAATCCCTCTTTTCTTCTCTCTTTCTCCATTTTGCCGCTTCTTTTTTTTTTTTTTTTTTAACTCTCTTCCTCTGCAACTGGCGTTTCCGAGAAATCCGCTGATAATGCCAAATCTGTATGATTCCCGAGAAACCCAAAAAGAAAAAAAGAAACCCCCATTTACTGCATTATTGGTTGTAAACCTGATTTGAGTTTTAATGATATCATATTGCGAACAGACGGAAAAGCTGAGGATGGAAATGGAGGAAAGAAAGAAGAGGCAATCGAGAATGCTGGTATCTGCGATAGAAGACAGAGTGGTGAAGAAGCTGAAGGAAAAAGACGAGGAGATAGAAAGAATGGGGAAACTGAACTGGGTTCTTCAAGAACGAGTGAAAAGACTGTGCATGGAGAATCAGCTATGGCGGGATTTGGCAGAGACAAACGAAGCCACCGTCAACACGCTCAGAACCAATCTGGAGCAGGTGCTGCTGGCGAACGTCGGCGAGGAGGGGCAGGCGCCGGCGGAGGACGCAGAGTCGAGCTGCGGGAGCAACGATTACGGGAGGGAAGCGGAGAGCCAAGAGGAGGTTGGCGGCGACGGAGGAGGGCGGTGCCGGAAATGTAGGGCGGGGGAGTCGAGGGTGCTGCTGTTGCCATGCAGGCATCTTTGCCTGTGTACGATGTGTGGGTCCACACTTCACACTTGCCCTGTATGCAATTCTGCCATCAATGCCAGCGTGCATGTTAATTTCTCTTAA

mRNA sequence

ATGGCAGTTGAAGCACGGCGTATGAGTCTTCTACCTTCTCAGTTCATCACGAACAGCAGAGATTTCTTGAAACCGAATCAAGGAAGCGTGGATATGATCTGCAACAGCAGCACCCAGATGACGACGGCGGGATTATTGCCTTACGCGGCGGCCAAAGTGCCGCCGGAGACTGTTCTCCCGTTCTACCAATCTTCTGCTTGCGATTTTGGGGGTTCGGGAAAGGCCGACAGTGGGCTCACTTCTAATTTCCCGGCCGCTCCCCGAAAGAGAACCAGACGGTCGGTTTACGATGAACCAGTCGTCGGTCGGGTTTCCGGCTTGTTCGATCAAGAAATCATCTCCTCCCAGATCCAACACCAACAGTCTGAGATCGATCGCTTCATTGCAATTCACACGGAAAAGCTGAGGATGGAAATGGAGGAAAGAAAGAAGAGGCAATCGAGAATGCTGGTATCTGCGATAGAAGACAGAGTGGTGAAGAAGCTGAAGGAAAAAGACGAGGAGATAGAAAGAATGGGGAAACTGAACTGGGTTCTTCAAGAACGAGTGAAAAGACTGTGCATGGAGAATCAGCTATGGCGGGATTTGGCAGAGACAAACGAAGCCACCGTCAACACGCTCAGAACCAATCTGGAGCAGGTGCTGCTGGCGAACGTCGGCGAGGAGGGGCAGGCGCCGGCGGAGGACGCAGAGTCGAGCTGCGGGAGCAACGATTACGGGAGGGAAGCGGAGAGCCAAGAGGAGGTTGGCGGCGACGGAGGAGGGCGGTGCCGGAAATGTAGGGCGGGGGAGTCGAGGGTGCTGCTGTTGCCATGCAGGCATCTTTGCCTGTGTACGATGTGTGGGTCCACACTTCACACTTGCCCTGTATGCAATTCTGCCATCAATGCCAGCGTGCATGTTAATTTCTCTTAA

Coding sequence (CDS)

ATGGCAGTTGAAGCACGGCGTATGAGTCTTCTACCTTCTCAGTTCATCACGAACAGCAGAGATTTCTTGAAACCGAATCAAGGAAGCGTGGATATGATCTGCAACAGCAGCACCCAGATGACGACGGCGGGATTATTGCCTTACGCGGCGGCCAAAGTGCCGCCGGAGACTGTTCTCCCGTTCTACCAATCTTCTGCTTGCGATTTTGGGGGTTCGGGAAAGGCCGACAGTGGGCTCACTTCTAATTTCCCGGCCGCTCCCCGAAAGAGAACCAGACGGTCGGTTTACGATGAACCAGTCGTCGGTCGGGTTTCCGGCTTGTTCGATCAAGAAATCATCTCCTCCCAGATCCAACACCAACAGTCTGAGATCGATCGCTTCATTGCAATTCACACGGAAAAGCTGAGGATGGAAATGGAGGAAAGAAAGAAGAGGCAATCGAGAATGCTGGTATCTGCGATAGAAGACAGAGTGGTGAAGAAGCTGAAGGAAAAAGACGAGGAGATAGAAAGAATGGGGAAACTGAACTGGGTTCTTCAAGAACGAGTGAAAAGACTGTGCATGGAGAATCAGCTATGGCGGGATTTGGCAGAGACAAACGAAGCCACCGTCAACACGCTCAGAACCAATCTGGAGCAGGTGCTGCTGGCGAACGTCGGCGAGGAGGGGCAGGCGCCGGCGGAGGACGCAGAGTCGAGCTGCGGGAGCAACGATTACGGGAGGGAAGCGGAGAGCCAAGAGGAGGTTGGCGGCGACGGAGGAGGGCGGTGCCGGAAATGTAGGGCGGGGGAGTCGAGGGTGCTGCTGTTGCCATGCAGGCATCTTTGCCTGTGTACGATGTGTGGGTCCACACTTCACACTTGCCCTGTATGCAATTCTGCCATCAATGCCAGCGTGCATGTTAATTTCTCTTAA

Protein sequence

MAVEARRMSLLPSQFITNSRDFLKPNQGSVDMICNSSTQMTTAGLLPYAAAKVPPETVLPFYQSSACDFGGSGKADSGLTSNFPAAPRKRTRRSVYDEPVVGRVSGLFDQEIISSQIQHQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEEIERMGKLNWVLQERVKRLCMENQLWRDLAETNEATVNTLRTNLEQVLLANVGEEGQAPAEDAESSCGSNDYGREAESQEEVGGDGGGRCRKCRAGESRVLLLPCRHLCLCTMCGSTLHTCPVCNSAINASVHVNFS

Homology

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BLAST of Sgr020815 vs. NCBI nr
Match: XP_038893892.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Benincasa hispida])

HSP 1 Score: 378.3 bits (970), Expect = 6.4e-101
Identity = 229/321 (71.34%), Postives = 254/321 (79.13%), Query Frame = 0


Query: 1   MAVEARRMSLLPSQF-ITNSRDFLKPNQGSVDMICN----SSTQMT----TAGLLPYAAA 60
           MAVEARRM+LLPSQF I N+RDF+K NQG VD ICN    ++TQM     T GLLPYAA 
Sbjct: 1   MAVEARRMNLLPSQFIIPNNRDFIKQNQGIVD-ICNNHNKTNTQMAPATETPGLLPYAA- 60

Query: 61  KVPPETVLPFYQSSACDFGGSGKADSGLTS-NFP--AAPRKRTRRSVYDEPVVGRVSGLF 120
            +PPETV  F   +  D+   GKA+SGLTS NFP  A  RKRTRRSVYDEP       L 
Sbjct: 61  -LPPETVNNF---AMYDW---GKAESGLTSNNFPTVAPSRKRTRRSVYDEPGGSFPGRLL 120

Query: 121 DQEIISSQIQHQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEE 180
           D+EII+S IQ QQSEIDRFIAIH EK+RMEME RKKR+S MLV AIE+RVVKKLKEK+EE
Sbjct: 121 DEEIINSHIQQQQSEIDRFIAIHREKVRMEMEVRKKRESGMLVRAIEERVVKKLKEKEEE 180

Query: 181 IERMGKLNWVLQERVKRLCMENQLWRDLAETNEATVNTLRTNLEQVLLANVGEEGQAPAE 240
           IERMGKLNWVLQERVKRLC+ENQ+WRDLAE+NEATVN+LR+NLEQV+LA       A AE
Sbjct: 181 IERMGKLNWVLQERVKRLCIENQVWRDLAESNEATVNSLRSNLEQVMLA-ANNNVVAAAE 240

Query: 241 DAESSCGS-NDYGREAESQEEV----GGDGGGRCRKCRAGESRVLLLPCRHLCLCTMCGS 300
           +AESSCGS +D GR    +EE     GG GGGRCRKC AGESRVL+LPCRHLCLCTMCGS
Sbjct: 241 EAESSCGSTSDCGRRGAEEEEESGGGGGAGGGRCRKCGAGESRVLVLPCRHLCLCTMCGS 300

Query: 301 TLHTCPVCNSAINASVHVNFS 305
           TLHTCPVCNSAINASVHVNFS
Sbjct: 301 TLHTCPVCNSAINASVHVNFS 311

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BLAST of Sgr020815 vs. NCBI nr
Match: XP_023001155.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima])

HSP 1 Score: 358.2 bits (918), Expect = 6.9e-95
Identity = 210/307 (68.40%), Postives = 231/307 (75.24%), Query Frame = 0


Query: 1   MAVEARRMSLLPSQFITNSRDFLKPNQGSVDMICNSSTQ-MTTAGLLPYAAAKVPPETVL 60
           MAVEARRM+ LPS FITN+ DF+K +Q  +D ICN  T    TAGL PY + K  PE V 
Sbjct: 1   MAVEARRMNHLPSPFITNNGDFMKQSQEVLD-ICNYKTMPAETAGLWPYISGKSVPEIVN 60

Query: 61  PFYQSSACDFGGSGKADSGLTS-NFPAAP-RKRTRRSVYDEPVVGRVSGLFDQEIISSQI 120
            F             ADSGLTS NFPAAP RKR RRS Y+EP       L D+EII+S +
Sbjct: 61  NF------------PADSGLTSNNFPAAPVRKRQRRSGYEEPPCPFPCRLSDEEIINSHV 120

Query: 121 QHQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEEIERMGKLNW 180
           Q QQSEIDRFIAIH EK+RM ME RKKR+S MLV AIE+R+VKKLKEKDEEIERMGKLNW
Sbjct: 121 QQQQSEIDRFIAIHREKVRMAMEVRKKRESGMLVRAIEERLVKKLKEKDEEIERMGKLNW 180

Query: 181 VLQERVKRLCMENQLWRDLAETNEATVNTLRTNLEQVLLANVGEEGQAPAEDAESSCGSN 240
           VLQERVKRLCMENQLWRDLAETNEATVN+LRTNLE+++ A    E  A   DAESSCGSN
Sbjct: 181 VLQERVKRLCMENQLWRDLAETNEATVNSLRTNLERLISA--ANETAAAGNDAESSCGSN 240

Query: 241 DYGREAESQEEVGGDGGGRCRKCRAGESRVLLLPCRHLCLCTMCGSTLHTCPVCNSAINA 300
                 E +EE    GG RCRKC AGESRVL+LPCRHLCLCTMCGSTLHTCPVC+SAINA
Sbjct: 241 RGNGMTEEEEEEESGGGRRCRKCMAGESRVLVLPCRHLCLCTMCGSTLHTCPVCDSAINA 292

Query: 301 SVHVNFS 305
           SVHVNFS
Sbjct: 301 SVHVNFS 292

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BLAST of Sgr020815 vs. NCBI nr
Match: XP_023519053.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 351.3 bits (900), Expect = 8.4e-93
Identity = 210/307 (68.40%), Postives = 233/307 (75.90%), Query Frame = 0


Query: 1   MAVEARRMSLLPSQFITNSRDFLKPNQGSVDMICNSSTQ-MTTAGLLPYAAAKVPPETVL 60
           MAVEARRM+ LPS FITN+ DF+K +Q  +D ICN  T  + TAGL PY A K  PE V 
Sbjct: 1   MAVEARRMNHLPSPFITNNGDFMKQSQEVLD-ICNYKTMPVETAGLWPYVAGKSVPEIVN 60

Query: 61  PFYQSSACDFGGSGKADSGLTS-NFPAAP-RKRTRRSVYDEPVVGRVSGLFDQEIISSQI 120
            F             ADSGLTS NFPAAP RKR RRS Y+EP       L D+EII+S +
Sbjct: 61  NF------------PADSGLTSNNFPAAPARKRQRRSGYEEPPCPFPCRLSDEEIINSHV 120

Query: 121 QHQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEEIERMGKLNW 180
           Q QQSEIDRFIAIH EK+RM ME RKKR+S MLV AIE+R+VKKLKEKDEEIERMGKLNW
Sbjct: 121 QQQQSEIDRFIAIHREKVRMAMEIRKKRESGMLVRAIEERLVKKLKEKDEEIERMGKLNW 180

Query: 181 VLQERVKRLCMENQLWRDLAETNEATVNTLRTNLEQVLLANVGEEGQAPAEDAESSCGSN 240
           VLQERVKRLCMENQLWRDLAETNEATVN+LRTNLE+++ A    +    A DAESSCGSN
Sbjct: 181 VLQERVKRLCMENQLWRDLAETNEATVNSLRTNLERLISA--ANDTATAANDAESSCGSN 240

Query: 241 DYGREAESQEEVGGDGGGRCRKCRAGESRVLLLPCRHLCLCTMCGSTLHTCPVCNSAINA 300
                  ++EE G  GG RCRKC AGESRVL+LPCRHLCLCTMCGSTLHTCPVC+SAINA
Sbjct: 241 --SGCGITEEESG--GGRRCRKCMAGESRVLVLPCRHLCLCTMCGSTLHTCPVCDSAINA 288

Query: 301 SVHVNFS 305
           SVHVNFS
Sbjct: 301 SVHVNFS 288

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BLAST of Sgr020815 vs. NCBI nr
Match: XP_022927535.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita moschata] >KAG6583800.1 BOI-related E3 ubiquitin-protein ligase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019425.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 351.3 bits (900), Expect = 8.4e-93
Identity = 207/306 (67.65%), Postives = 232/306 (75.82%), Query Frame = 0


Query: 1   MAVEARRMSLLPSQFITNSRDFLKPNQGSVDMICNSSTQMTTAGLLPYAAAKVPPETVLP 60
           MAVEARRM+ LPS FITN+ DF+K +Q  +D+    +  + TAGL PY A K  PE V  
Sbjct: 1   MAVEARRMNHLPSPFITNNGDFMKQSQEVLDIYNYKTMPVETAGLWPYVAGKSVPEIVNN 60

Query: 61  FYQSSACDFGGSGKADSGLTS-NFPAAP-RKRTRRSVYDEPVVGRVSGLFDQEIISSQIQ 120
           F             ADSGLTS NFPAAP RKR RRS Y+EP       L D+EII+S +Q
Sbjct: 61  F------------PADSGLTSNNFPAAPVRKRQRRSGYEEPPCPFPCRLSDEEIINSHVQ 120

Query: 121 HQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEEIERMGKLNWV 180
            QQSEIDRFIAIH EK+RM ME RKKR+S MLV AIE+R+VKKLKEKDEEIERMGKLNWV
Sbjct: 121 QQQSEIDRFIAIHREKVRMAMEIRKKRESGMLVRAIEERLVKKLKEKDEEIERMGKLNWV 180

Query: 181 LQERVKRLCMENQLWRDLAETNEATVNTLRTNLEQVLLANVGEEGQAPAEDAESSCGSND 240
           LQERVKRLCMENQLWRDLAETNEATVN+LRTNLE+++ A    +  A A DAESSCGSN 
Sbjct: 181 LQERVKRLCMENQLWRDLAETNEATVNSLRTNLERLISA--ANDTAAAANDAESSCGSN- 240

Query: 241 YGREAESQEEVGGDGGGRCRKCRAGESRVLLLPCRHLCLCTMCGSTLHTCPVCNSAINAS 300
                 ++EE G  GG RCRKC AGESRVL+LPCRHLCLCTMCGSTLHTCPVC+SAINAS
Sbjct: 241 -SGCGITEEESG--GGRRCRKCMAGESRVLVLPCRHLCLCTMCGSTLHTCPVCDSAINAS 288

Query: 301 VHVNFS 305
           VHVNFS
Sbjct: 301 VHVNFS 288

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BLAST of Sgr020815 vs. NCBI nr
Match: XP_008459064.1 (PREDICTED: E3 ubiquitin-protein ligase BOI-like [Cucumis melo])

HSP 1 Score: 347.1 bits (889), Expect = 1.6e-91
Identity = 213/320 (66.56%), Postives = 242/320 (75.62%), Query Frame = 0


Query: 1   MAVEARRMSLLPSQ--FITNSRDFLKPNQGSVDMICNSSTQMTTAGLL-PYAAAK--VPP 60
           MAVEAR M+LLPS    I N+RDF+K NQ         +T  TT G++ PY A K  V P
Sbjct: 1   MAVEARPMNLLPSHQFIIPNNRDFIKQNQELASTHMTPTTDTTTPGIIWPYTAVKPAVTP 60

Query: 61  ETVLPFYQSSACDFGGSGKADSGLTS-NFP--AAPRKRTR-RSVYDEPVVGRVSGLFDQE 120
           E  L  + ++  D+   GKA+SGLTS NFP  A  RKRTR RS YDEP  GR   L D+E
Sbjct: 61  EATLNNFATTMYDW---GKAESGLTSNNFPSTAPTRKRTRSRSFYDEP-GGR---LLDEE 120

Query: 121 IISSQIQHQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEEIER 180
           II+S IQ QQSE+DRFIAIH EK+R+EME RKKR+S MLV AIE+RVVKKLKEK+EEIER
Sbjct: 121 IINSHIQQQQSEMDRFIAIHREKMRIEMEIRKKRESGMLVRAIEERVVKKLKEKEEEIER 180

Query: 181 MGKLNWVLQERVKRLCMENQLWRDLAETNEATVNTLRTNLEQVLL--ANVGEEGQAPA-- 240
           MGKLNWVLQERVKRLCMENQ+WRDLAE+NEATVN LR NLEQV+L  AN    G A A  
Sbjct: 181 MGKLNWVLQERVKRLCMENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKE 240

Query: 241 --EDAESSCGS-NDYGREAESQEEVGGDGGGRCRKCRAGESRVLLLPCRHLCLCTMCGST 300
             E AESSCGS ++ GR+ E +EE    GGGRCRKC AGESRVL+LPCRHLCLCTMCGST
Sbjct: 241 KEEKAESSCGSTSECGRKEEEEEEAESGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGST 300

Query: 301 LHTCPVCNSAINASVHVNFS 305
           +H+CPVCNSAINASVHVNFS
Sbjct: 301 IHSCPVCNSAINASVHVNFS 313

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BLAST of Sgr020815 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.1e-54
Identity = 145/314 (46.18%), Postives = 188/314 (59.87%), Query Frame = 0


Query: 1   MAVEARRMSLLPSQFITNSRDFLKPNQGSVDMICNSSTQMTTAGLLPYAAAKVPPETVLP 60
           MAVEAR M+L  SQ+ITN R+ +K           ++ Q    G  P        + + P
Sbjct: 1   MAVEARHMNLFSSQYITN-RECVKSQTNM------NNGQQIAGGGFPVTIGDRNLQYIDP 60

Query: 61  F--YQSSACDFGGSGKADSGLTSNFPAAPRKRTRRSVYDEPVVGRVSGLFDQEIISSQIQ 120
              +  S  +     K     T +  A    + RR++   P     + L D E++ SQIQ
Sbjct: 61  INSFNKSESELTAISKRQRDSTFDSDALIASQKRRAIAFSP-----ASLIDAELV-SQIQ 120

Query: 121 HQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEEIERMGKLNWV 180
            Q SEIDRF+A  TE LR+E+E R++ Q+RML SA+++ ++KKLK KDEEI RMGKLNWV
Sbjct: 121 QQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKLNWV 180

Query: 181 LQERVKRLCMENQLWRDLAETNEATVNTLRTNLEQVLLANVGEEGQ------APAEDAES 240
           LQERVK L +ENQ+WRDLA+TNEAT N LR+NLEQV LA V +           A+DAES
Sbjct: 181 LQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQV-LAQVDDLDAFRRPLVEEADDAES 240

Query: 241 SCGSNDYGREAESQEEVGGDGGGRCRKCRAGESRVLLLPCRHLCLCTMCGST--LHTCPV 300
           SCGS D G       +V     G C++C    + VL+LPCRHLCLCT+CGS+  L TCPV
Sbjct: 241 SCGSCDGG-------DVTAVVNGGCKRCGELTASVLVLPCRHLCLCTVCGSSALLRTCPV 293

Query: 301 CNSAINASVHVNFS 305
           C+  + ASVHVN S
Sbjct: 301 CDMVMTASVHVNMS 293

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BLAST of Sgr020815 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.0e-53
Identity = 145/322 (45.03%), Postives = 186/322 (57.76%), Query Frame = 0


Query: 1   MAVEARRMSLLPSQFITNSRDFLKPNQGSVDMICNSSTQMTTAGLLPYAAAKVPPETVLP 60
           MAV+A  M++  SQFI+ +RD            C    +    G   +   +VP  T   
Sbjct: 1   MAVQAHHMNIF-SQFISPNRD------------CVKFQENMNHGEFEFTGGEVPLITGES 60

Query: 61  FYQSSACDFGGSGKADSGLTSNFPAAP----RKRTRRSVYDEPVVGRVSGLFDQ------ 120
           F            KA+SGL+ NF   P    R+R  +       V R S  FD       
Sbjct: 61  FAVEPLAAKANFNKAESGLSYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPSLI 120

Query: 121 --EIISSQIQHQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEE 180
             E++S     QQSEIDRF+A  TEKLR+E+E R++ Q+RML SA+++ + KKLKEKD+E
Sbjct: 121 NVELVSQIQNQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDE 180

Query: 181 IERMGKLNWVLQERVKRLCMENQLWRDLAETNEATVNTLRTNLEQVL--LANVGEEGQAP 240
           I R+  LNWVLQERVK L +ENQ+WRD+A+TNEA  NTLRTNL+QVL  L          
Sbjct: 181 IVRIRNLNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQLETFPTASAVV 240

Query: 241 AEDAESSCGS--NDYGREAESQEEVGGDGGGRCRKCRAGESRVLLLPCRHLCLCTMCGST 300
            +DAESSCGS   D G EA +        GG C++C   E+ VL+LPCRHLCLCT+CG +
Sbjct: 241 EDDAESSCGSCCGDGGGEAVTAV------GGGCKRCGEREASVLVLPCRHLCLCTVCGGS 300

Query: 301 --LHTCPVCNSAINASVHVNFS 305
             L TCPVC+  +NASVHVN S
Sbjct: 301 ALLRTCPVCDMVMNASVHVNMS 303

[Close]

BLAST of Sgr020815 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 9.2e-50
Identity = 143/346 (41.33%), Postives = 199/346 (57.51%), Query Frame = 0


Query: 1   MAVEARRMSLLPSQFITNSRDFLKPNQGSVDMICNSSTQMTTAGLLPYAAAKVPPETVL- 60
           MAVEA  ++ L     +++R+ + P + S  ++ N  TQM   G +P     V  +T L 
Sbjct: 1   MAVEAHHLNPL----FSSNREMIHPVEAS-GVVYN--TQM-RYGTVPTFNPTVECQTSLF 60

Query: 61  -PFYQSSACD--FGGSGK-----ADSGLTSNF--------PAAPRKRTRRSVYDEPVVGR 120
            P Y  S  D     S K      DS LT N         P + RKR+R    +E VV  
Sbjct: 61  NPIYNISPVDRLVHQSMKPTIQSVDSSLTFNSDNNVDFLRPVSSRKRSR----EESVVLN 120

Query: 121 VSG------------LFDQEIISSQIQHQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLV 180
            S             +F  + +SS +Q    +IDR I+ H E++RME+EE++K Q R +V
Sbjct: 121 PSAYMQIQKNPTDPLMFLGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRIV 180

Query: 181 SAIEDRVVKKLKEKDEEIERMGKLNWVLQERVKRLCMENQLWRDLAETNEATVNTLRTNL 240
            A+E  ++K L+ KD+EI  +GKLN  L+E+VK LC+ENQ+WRD+A++NEATVN LR+NL
Sbjct: 181 EAVEQGLMKTLRAKDDEINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSNL 240

Query: 241 EQVLLA---NVGEEGQAPAEDAESSCGSNDYGREAESQEEVGGD----------GGGRCR 300
           +QVL A   N  EE    A+DA+S CGSND G   E + ++ G+          G   CR
Sbjct: 241 QQVLAAVERNRWEEPPTVADDAQSCCGSNDEGDSEEERWKLAGEAQDTKKMCRVGMSMCR 300

Query: 301 KCRAGESRVLLLPCRHLCLCTMCGSTLHTCPVCNSAINASVHVNFS 305
            C  GE+ VLLLPCRH+CLC++CGS+L+TCP+C S   AS+HVN S
Sbjct: 301 SCGKGEASVLLLPCRHMCLCSVCGSSLNTCPICKSPKTASLHVNLS 334

[Close]

BLAST of Sgr020815 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 2.6e-44
Identity = 108/213 (50.70%), Postives = 143/213 (67.14%), Query Frame = 0


Query: 113 ISSQIQHQQSEIDRFIAIH---TEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEEI 172
           ISS +  QQ EIDRF+++H    E+++ E+EE++KRQ+R ++ AIE  +VK+L+ K+EE 
Sbjct: 145 ISSHMNQQQHEIDRFVSLHLYQMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEER 204

Query: 173 ERMGKLNWVLQERVKRLCMENQLWRDLAETNEATVNTLRTNLEQVL--LANVG-----EE 232
           ER+GK+N  L+ERVK L +ENQ+WRDLA+TNEAT N LRTNLE VL  + +V      E+
Sbjct: 205 ERIGKVNHALEERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEK 264

Query: 233 GQAPAEDAESSCGSN-----------DYGREAESQEEVGGDGGGRCRKCRAGESRVLLLP 292
                +DAES CGS+             G E E+Q++        CR C   ES VLLLP
Sbjct: 265 NMNEEDDAESCCGSSCGGGGEETVRRRVGLEREAQDKAERRRRRMCRNCGEEESCVLLLP 324

Query: 293 CRHLCLCTMCGSTLHTCPVCNSAINASVHVNFS 305
           CRHLCLC +CGS++HTCP+C S  NASVHVN S
Sbjct: 325 CRHLCLCGVCGSSVHTCPICTSPKNASVHVNMS 357

[Close]

BLAST of Sgr020815 vs. ExPASy TrEMBL
Match: A0A6J1KKD3 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111495377 PE=4 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 3.3e-95
Identity = 210/307 (68.40%), Postives = 231/307 (75.24%), Query Frame = 0


Query: 1   MAVEARRMSLLPSQFITNSRDFLKPNQGSVDMICNSSTQ-MTTAGLLPYAAAKVPPETVL 60
           MAVEARRM+ LPS FITN+ DF+K +Q  +D ICN  T    TAGL PY + K  PE V 
Sbjct: 1   MAVEARRMNHLPSPFITNNGDFMKQSQEVLD-ICNYKTMPAETAGLWPYISGKSVPEIVN 60

Query: 61  PFYQSSACDFGGSGKADSGLTS-NFPAAP-RKRTRRSVYDEPVVGRVSGLFDQEIISSQI 120
            F             ADSGLTS NFPAAP RKR RRS Y+EP       L D+EII+S +
Sbjct: 61  NF------------PADSGLTSNNFPAAPVRKRQRRSGYEEPPCPFPCRLSDEEIINSHV 120

Query: 121 QHQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEEIERMGKLNW 180
           Q QQSEIDRFIAIH EK+RM ME RKKR+S MLV AIE+R+VKKLKEKDEEIERMGKLNW
Sbjct: 121 QQQQSEIDRFIAIHREKVRMAMEVRKKRESGMLVRAIEERLVKKLKEKDEEIERMGKLNW 180

Query: 181 VLQERVKRLCMENQLWRDLAETNEATVNTLRTNLEQVLLANVGEEGQAPAEDAESSCGSN 240
           VLQERVKRLCMENQLWRDLAETNEATVN+LRTNLE+++ A    E  A   DAESSCGSN
Sbjct: 181 VLQERVKRLCMENQLWRDLAETNEATVNSLRTNLERLISA--ANETAAAGNDAESSCGSN 240

Query: 241 DYGREAESQEEVGGDGGGRCRKCRAGESRVLLLPCRHLCLCTMCGSTLHTCPVCNSAINA 300
                 E +EE    GG RCRKC AGESRVL+LPCRHLCLCTMCGSTLHTCPVC+SAINA
Sbjct: 241 RGNGMTEEEEEEESGGGRRCRKCMAGESRVLVLPCRHLCLCTMCGSTLHTCPVCDSAINA 292

Query: 301 SVHVNFS 305
           SVHVNFS
Sbjct: 301 SVHVNFS 292

[Close]

BLAST of Sgr020815 vs. ExPASy TrEMBL
Match: A0A6J1EP88 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111434333 PE=4 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 4.1e-93
Identity = 207/306 (67.65%), Postives = 232/306 (75.82%), Query Frame = 0


Query: 1   MAVEARRMSLLPSQFITNSRDFLKPNQGSVDMICNSSTQMTTAGLLPYAAAKVPPETVLP 60
           MAVEARRM+ LPS FITN+ DF+K +Q  +D+    +  + TAGL PY A K  PE V  
Sbjct: 1   MAVEARRMNHLPSPFITNNGDFMKQSQEVLDIYNYKTMPVETAGLWPYVAGKSVPEIVNN 60

Query: 61  FYQSSACDFGGSGKADSGLTS-NFPAAP-RKRTRRSVYDEPVVGRVSGLFDQEIISSQIQ 120
           F             ADSGLTS NFPAAP RKR RRS Y+EP       L D+EII+S +Q
Sbjct: 61  F------------PADSGLTSNNFPAAPVRKRQRRSGYEEPPCPFPCRLSDEEIINSHVQ 120

Query: 121 HQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEEIERMGKLNWV 180
            QQSEIDRFIAIH EK+RM ME RKKR+S MLV AIE+R+VKKLKEKDEEIERMGKLNWV
Sbjct: 121 QQQSEIDRFIAIHREKVRMAMEIRKKRESGMLVRAIEERLVKKLKEKDEEIERMGKLNWV 180

Query: 181 LQERVKRLCMENQLWRDLAETNEATVNTLRTNLEQVLLANVGEEGQAPAEDAESSCGSND 240
           LQERVKRLCMENQLWRDLAETNEATVN+LRTNLE+++ A    +  A A DAESSCGSN 
Sbjct: 181 LQERVKRLCMENQLWRDLAETNEATVNSLRTNLERLISA--ANDTAAAANDAESSCGSN- 240

Query: 241 YGREAESQEEVGGDGGGRCRKCRAGESRVLLLPCRHLCLCTMCGSTLHTCPVCNSAINAS 300
                 ++EE G  GG RCRKC AGESRVL+LPCRHLCLCTMCGSTLHTCPVC+SAINAS
Sbjct: 241 -SGCGITEEESG--GGRRCRKCMAGESRVLVLPCRHLCLCTMCGSTLHTCPVCDSAINAS 288

Query: 301 VHVNFS 305
           VHVNFS
Sbjct: 301 VHVNFS 288

[Close]

BLAST of Sgr020815 vs. ExPASy TrEMBL
Match: A0A1S3C8U5 (E3 ubiquitin-protein ligase BOI-like OS=Cucumis melo OX=3656 GN=LOC103498284 PE=4 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 7.7e-92
Identity = 213/320 (66.56%), Postives = 242/320 (75.62%), Query Frame = 0


Query: 1   MAVEARRMSLLPSQ--FITNSRDFLKPNQGSVDMICNSSTQMTTAGLL-PYAAAK--VPP 60
           MAVEAR M+LLPS    I N+RDF+K NQ         +T  TT G++ PY A K  V P
Sbjct: 1   MAVEARPMNLLPSHQFIIPNNRDFIKQNQELASTHMTPTTDTTTPGIIWPYTAVKPAVTP 60

Query: 61  ETVLPFYQSSACDFGGSGKADSGLTS-NFP--AAPRKRTR-RSVYDEPVVGRVSGLFDQE 120
           E  L  + ++  D+   GKA+SGLTS NFP  A  RKRTR RS YDEP  GR   L D+E
Sbjct: 61  EATLNNFATTMYDW---GKAESGLTSNNFPSTAPTRKRTRSRSFYDEP-GGR---LLDEE 120

Query: 121 IISSQIQHQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEEIER 180
           II+S IQ QQSE+DRFIAIH EK+R+EME RKKR+S MLV AIE+RVVKKLKEK+EEIER
Sbjct: 121 IINSHIQQQQSEMDRFIAIHREKMRIEMEIRKKRESGMLVRAIEERVVKKLKEKEEEIER 180

Query: 181 MGKLNWVLQERVKRLCMENQLWRDLAETNEATVNTLRTNLEQVLL--ANVGEEGQAPA-- 240
           MGKLNWVLQERVKRLCMENQ+WRDLAE+NEATVN LR NLEQV+L  AN    G A A  
Sbjct: 181 MGKLNWVLQERVKRLCMENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKE 240

Query: 241 --EDAESSCGS-NDYGREAESQEEVGGDGGGRCRKCRAGESRVLLLPCRHLCLCTMCGST 300
             E AESSCGS ++ GR+ E +EE    GGGRCRKC AGESRVL+LPCRHLCLCTMCGST
Sbjct: 241 KEEKAESSCGSTSECGRKEEEEEEAESGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGST 300

Query: 301 LHTCPVCNSAINASVHVNFS 305
           +H+CPVCNSAINASVHVNFS
Sbjct: 301 IHSCPVCNSAINASVHVNFS 313

[Close]

BLAST of Sgr020815 vs. ExPASy TrEMBL
Match: A0A0A0M3V5 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G707120 PE=4 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 4.2e-90
Identity = 214/321 (66.67%), Postives = 243/321 (75.70%), Query Frame = 0


Query: 1   MAVEARRMSLLPSQ--FITNSRDFLKPNQGSVDMICNSSTQMTTAGLL-PYAAAK--VPP 60
           MAVEAR M+LLPS    I N RDF+K NQ   +      T  TT G++ PY A K  V P
Sbjct: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60

Query: 61  ETVLPFYQSSACDFGGSGKADSGLTS-NFP--AAPRKRTR-RSVYDEPVVGRVSGLFDQE 120
           ET L  + ++  D+   GKA+SGLTS NFP  A  RKRTR RS YDEP  GR   L D+E
Sbjct: 61  ETTLNNF-ATVYDW---GKAESGLTSNNFPSTAPTRKRTRSRSFYDEP-GGR---LLDEE 120

Query: 121 IISSQIQHQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEEIER 180
           II+S IQ QQSE+DRFIAIH EK+R+EME RKKR+S MLV AIE+RVVKKLKEK+EEIER
Sbjct: 121 IINSHIQQQQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIER 180

Query: 181 MGKLNWVLQERVKRLCMENQLWRDLAETNEATVNTLRTNLEQVLL--ANVGEEGQAPA-- 240
           MGKLNWVLQERVKRLC+ENQ+WRDLAE+NEATVN LR NLEQV+L  AN    G A A  
Sbjct: 181 MGKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKE 240

Query: 241 --EDAESSCGS-NDYGREAESQEEV-GGDGGGRCRKCRAGESRVLLLPCRHLCLCTMCGS 300
             E AESSCGS ++ GR+ E +EE   G GGGRCRKC AGESRVL+LPCRHLCLCTMCGS
Sbjct: 241 KEEKAESSCGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGS 300

Query: 301 TLHTCPVCNSAINASVHVNFS 305
           T+H+CPVCNSAINASVHVNFS
Sbjct: 301 TIHSCPVCNSAINASVHVNFS 313

[Close]

BLAST of Sgr020815 vs. ExPASy TrEMBL
Match: A0A6J1GUS0 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111457113 PE=4 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 2.2e-83
Identity = 193/308 (62.66%), Postives = 212/308 (68.83%), Query Frame = 0


Query: 1   MAVEARRMSLLPS--QFITNSRDFLKPNQGSVDMICNSSTQMTTAGLLPYAAAKVPP--E 60
           MAVEAR ++L PS   FITN RDF+ PN                           PP   
Sbjct: 1   MAVEARPINLFPSTPHFITN-RDFIDPN-----------------------TPTAPPLHS 60

Query: 61  TVLPFYQSSACDFGGSGKADSGLTSNFPAAPRKRTRRSVYDEPVVGRVSGLFDQEIISSQ 120
              P Y          G  D+GLTS      RKR R SVYDE        LFDQE+I S 
Sbjct: 61  LTFPMY----------GNPDTGLTS---TPSRKRARCSVYDE----SPPRLFDQEMIISH 120

Query: 121 IQHQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEEIERMGKLN 180
           I+HQ+SEIDRFIAIHTEK+ MEMEERK+RQSRMLVSAIEDRVVKKLKEK+EEIERMGKLN
Sbjct: 121 IEHQRSEIDRFIAIHTEKVMMEMEERKQRQSRMLVSAIEDRVVKKLKEKEEEIERMGKLN 180

Query: 181 WVLQERVKRLCMENQLWRDLAETNEATVNTLRTNLEQVLLANVGEEGQAPAEDAESSCGS 240
           WVLQERVK L +ENQ WR+LAETNEAT+N+LRTNLEQVL+AN GEE  A A+DAESSCGS
Sbjct: 181 WVLQERVKHLSLENQQWRNLAETNEATLNSLRTNLEQVLMANAGEEAAAVADDAESSCGS 240

Query: 241 NDYGREAESQEEVGGDGGGRCRKCRAGESRVLLLPCRHLCLCTMCGSTLHTCPVCNSAIN 300
           +             G GGGRCRKC A ESRVL+LPCRHLCLCT+CGS LHTCPVCNSAIN
Sbjct: 241 S-----------YSGGGGGRCRKCGAAESRVLVLPCRHLCLCTICGSRLHTCPVCNSAIN 256

Query: 301 ASVHVNFS 305
           ASVHVNFS
Sbjct: 301 ASVHVNFS 256

[Close]

BLAST of Sgr020815 vs. TAIR 10
Match: AT5G45100.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 214.2 bits (544), Expect = 1.5e-55
Identity = 145/314 (46.18%), Postives = 188/314 (59.87%), Query Frame = 0


Query: 1   MAVEARRMSLLPSQFITNSRDFLKPNQGSVDMICNSSTQMTTAGLLPYAAAKVPPETVLP 60
           MAVEAR M+L  SQ+ITN R+ +K           ++ Q    G  P        + + P
Sbjct: 1   MAVEARHMNLFSSQYITN-RECVKSQTNM------NNGQQIAGGGFPVTIGDRNLQYIDP 60

Query: 61  F--YQSSACDFGGSGKADSGLTSNFPAAPRKRTRRSVYDEPVVGRVSGLFDQEIISSQIQ 120
              +  S  +     K     T +  A    + RR++   P     + L D E++ SQIQ
Sbjct: 61  INSFNKSESELTAISKRQRDSTFDSDALIASQKRRAIAFSP-----ASLIDAELV-SQIQ 120

Query: 121 HQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEEIERMGKLNWV 180
            Q SEIDRF+A  TE LR+E+E R++ Q+RML SA+++ ++KKLK KDEEI RMGKLNWV
Sbjct: 121 QQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKLNWV 180

Query: 181 LQERVKRLCMENQLWRDLAETNEATVNTLRTNLEQVLLANVGEEGQ------APAEDAES 240
           LQERVK L +ENQ+WRDLA+TNEAT N LR+NLEQV LA V +           A+DAES
Sbjct: 181 LQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQV-LAQVDDLDAFRRPLVEEADDAES 240

Query: 241 SCGSNDYGREAESQEEVGGDGGGRCRKCRAGESRVLLLPCRHLCLCTMCGST--LHTCPV 300
           SCGS D G       +V     G C++C    + VL+LPCRHLCLCT+CGS+  L TCPV
Sbjct: 241 SCGSCDGG-------DVTAVVNGGCKRCGELTASVLVLPCRHLCLCTVCGSSALLRTCPV 293

Query: 301 CNSAINASVHVNFS 305
           C+  + ASVHVN S
Sbjct: 301 CDMVMTASVHVNMS 293

[Close]

BLAST of Sgr020815 vs. TAIR 10
Match: AT4G19700.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 211.8 bits (538), Expect = 7.4e-55
Identity = 145/322 (45.03%), Postives = 186/322 (57.76%), Query Frame = 0


Query: 1   MAVEARRMSLLPSQFITNSRDFLKPNQGSVDMICNSSTQMTTAGLLPYAAAKVPPETVLP 60
           MAV+A  M++  SQFI+ +RD            C    +    G   +   +VP  T   
Sbjct: 1   MAVQAHHMNIF-SQFISPNRD------------CVKFQENMNHGEFEFTGGEVPLITGES 60

Query: 61  FYQSSACDFGGSGKADSGLTSNFPAAP----RKRTRRSVYDEPVVGRVSGLFDQ------ 120
           F            KA+SGL+ NF   P    R+R  +       V R S  FD       
Sbjct: 61  FAVEPLAAKANFNKAESGLSYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPSLI 120

Query: 121 --EIISSQIQHQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEE 180
             E++S     QQSEIDRF+A  TEKLR+E+E R++ Q+RML SA+++ + KKLKEKD+E
Sbjct: 121 NVELVSQIQNQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDE 180

Query: 181 IERMGKLNWVLQERVKRLCMENQLWRDLAETNEATVNTLRTNLEQVL--LANVGEEGQAP 240
           I R+  LNWVLQERVK L +ENQ+WRD+A+TNEA  NTLRTNL+QVL  L          
Sbjct: 181 IVRIRNLNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQLETFPTASAVV 240

Query: 241 AEDAESSCGS--NDYGREAESQEEVGGDGGGRCRKCRAGESRVLLLPCRHLCLCTMCGST 300
            +DAESSCGS   D G EA +        GG C++C   E+ VL+LPCRHLCLCT+CG +
Sbjct: 241 EDDAESSCGSCCGDGGGEAVTAV------GGGCKRCGEREASVLVLPCRHLCLCTVCGGS 300

Query: 301 --LHTCPVCNSAINASVHVNFS 305
             L TCPVC+  +NASVHVN S
Sbjct: 301 ALLRTCPVCDMVMNASVHVNMS 303

[Close]

BLAST of Sgr020815 vs. TAIR 10
Match: AT5G45100.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 201.1 bits (510), Expect = 1.3e-51
Identity = 129/249 (51.81%), Postives = 162/249 (65.06%), Query Frame = 0


Query: 74  KADSGLTSNFPAAPRKRTRRSVYD----------EPVVGRVSGLFDQEIISSQIQHQQSE 133
           K++S LT     A  KR R S +D            +    + L D E++ SQIQ Q SE
Sbjct: 32  KSESELT-----AISKRQRDSTFDSDALIASQKRRAIAFSPASLIDAELV-SQIQQQNSE 91

Query: 134 IDRFIAIHTEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEEIERMGKLNWVLQERV 193
           IDRF+A  TE LR+E+E R++ Q+RML SA+++ ++KKLK KDEEI RMGKLNWVLQERV
Sbjct: 92  IDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKLNWVLQERV 151

Query: 194 KRLCMENQLWRDLAETNEATVNTLRTNLEQVLLANVGEEGQ------APAEDAESSCGSN 253
           K L +ENQ+WRDLA+TNEAT N LR+NLEQV LA V +           A+DAESSCGS 
Sbjct: 152 KNLYVENQIWRDLAQTNEATANNLRSNLEQV-LAQVDDLDAFRRPLVEEADDAESSCGSC 211

Query: 254 DYGREAESQEEVGGDGGGRCRKCRAGESRVLLLPCRHLCLCTMCGST--LHTCPVCNSAI 305
           D G       +V     G C++C    + VL+LPCRHLCLCT+CGS+  L TCPVC+  +
Sbjct: 212 DGG-------DVTAVVNGGCKRCGELTASVLVLPCRHLCLCTVCGSSALLRTCPVCDMVM 266

[Close]

BLAST of Sgr020815 vs. TAIR 10
Match: AT3G12920.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 198.7 bits (504), Expect = 6.5e-51
Identity = 143/346 (41.33%), Postives = 199/346 (57.51%), Query Frame = 0


Query: 1   MAVEARRMSLLPSQFITNSRDFLKPNQGSVDMICNSSTQMTTAGLLPYAAAKVPPETVL- 60
           MAVEA  ++ L     +++R+ + P + S  ++ N  TQM   G +P     V  +T L 
Sbjct: 1   MAVEAHHLNPL----FSSNREMIHPVEAS-GVVYN--TQM-RYGTVPTFNPTVECQTSLF 60

Query: 61  -PFYQSSACD--FGGSGK-----ADSGLTSNF--------PAAPRKRTRRSVYDEPVVGR 120
            P Y  S  D     S K      DS LT N         P + RKR+R    +E VV  
Sbjct: 61  NPIYNISPVDRLVHQSMKPTIQSVDSSLTFNSDNNVDFLRPVSSRKRSR----EESVVLN 120

Query: 121 VSG------------LFDQEIISSQIQHQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLV 180
            S             +F  + +SS +Q    +IDR I+ H E++RME+EE++K Q R +V
Sbjct: 121 PSAYMQIQKNPTDPLMFLGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRIV 180

Query: 181 SAIEDRVVKKLKEKDEEIERMGKLNWVLQERVKRLCMENQLWRDLAETNEATVNTLRTNL 240
            A+E  ++K L+ KD+EI  +GKLN  L+E+VK LC+ENQ+WRD+A++NEATVN LR+NL
Sbjct: 181 EAVEQGLMKTLRAKDDEINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSNL 240

Query: 241 EQVLLA---NVGEEGQAPAEDAESSCGSNDYGREAESQEEVGGD----------GGGRCR 300
           +QVL A   N  EE    A+DA+S CGSND G   E + ++ G+          G   CR
Sbjct: 241 QQVLAAVERNRWEEPPTVADDAQSCCGSNDEGDSEEERWKLAGEAQDTKKMCRVGMSMCR 300

Query: 301 KCRAGESRVLLLPCRHLCLCTMCGSTLHTCPVCNSAINASVHVNFS 305
            C  GE+ VLLLPCRH+CLC++CGS+L+TCP+C S   AS+HVN S
Sbjct: 301 SCGKGEASVLLLPCRHMCLCSVCGSSLNTCPICKSPKTASLHVNLS 334

[Close]

BLAST of Sgr020815 vs. TAIR 10
Match: AT1G79110.2 (zinc ion binding )

HSP 1 Score: 186.0 bits (471), Expect = 4.4e-47
Identity = 108/210 (51.43%), Postives = 143/210 (68.10%), Query Frame = 0


Query: 113 ISSQIQHQQSEIDRFIAIHTEKLRMEMEERKKRQSRMLVSAIEDRVVKKLKEKDEEIERM 172
           ISS +  QQ EIDRF+++H E+++ E+EE++KRQ+R ++ AIE  +VK+L+ K+EE ER+
Sbjct: 145 ISSHMNQQQHEIDRFVSLHMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERI 204

Query: 173 GKLNWVLQERVKRLCMENQLWRDLAETNEATVNTLRTNLEQVL--LANVG-----EEGQA 232
           GK+N  L+ERVK L +ENQ+WRDLA+TNEAT N LRTNLE VL  + +V      E+   
Sbjct: 205 GKVNHALEERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMN 264

Query: 233 PAEDAESSCGSN-----------DYGREAESQEEVGGDGGGRCRKCRAGESRVLLLPCRH 292
             +DAES CGS+             G E E+Q++        CR C   ES VLLLPCRH
Sbjct: 265 EEDDAESCCGSSCGGGGEETVRRRVGLEREAQDKAERRRRRMCRNCGEEESCVLLLPCRH 324

Query: 293 LCLCTMCGSTLHTCPVCNSAINASVHVNFS 305
           LCLC +CGS++HTCP+C S  NASVHVN S
Sbjct: 325 LCLCGVCGSSVHTCPICTSPKNASVHVNMS 354

The following BLAST results are available for this feature:

BLAST of Sgr020815 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893892.1	6.4e-101	71.34	BOI-related E3 ubiquitin-protein ligase 1-like [Benincasa hispida]	[more]
XP_023001155.1	6.9e-95	68.40	BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima]	[more]
XP_023519053.1	8.4e-93	68.40	BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022927535.1	8.4e-93	67.65	BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita moschata] >KAG6583800....	[more]
XP_008459064.1	1.6e-91	66.56	PREDICTED: E3 ubiquitin-protein ligase BOI-like [Cucumis melo]	[more]

BLAST of Sgr020815 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FHE4	2.1e-54	46.18	BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG...	[more]
O81851	1.0e-53	45.03	E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1	[more]
Q9LDD1	9.2e-50	41.33	Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37...	[more]
F4IDI6	2.6e-44	50.70	Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37...	[more]

BLAST of Sgr020815 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KKD3	3.3e-95	68.40	BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1EP88	4.1e-93	67.65	BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A1S3C8U5	7.7e-92	66.56	E3 ubiquitin-protein ligase BOI-like OS=Cucumis melo OX=3656 GN=LOC103498284 PE=...	[more]
A0A0A0M3V5	4.2e-90	66.67	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G707120 P...	[more]
A0A6J1GUS0	2.2e-83	62.66	BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]

BLAST of Sgr020815 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45100.1	1.5e-55	46.18	SBP (S-ribonuclease binding protein) family protein	[more]
AT4G19700.1	7.4e-55	45.03	SBP (S-ribonuclease binding protein) family protein	[more]
AT5G45100.2	1.3e-51	51.81	SBP (S-ribonuclease binding protein) family protein	[more]
AT3G12920.1	6.5e-51	41.33	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G79110.2	4.4e-47	51.43	zinc ion binding	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 256..296 e-value: 8.0E-9 score: 35.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 241..255
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 222..255
None	No IPR available	PANTHER	PTHR42647:SF55	BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 1	coord: 1..303
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 233..303 e-value: 1.0E-11 score: 46.1
IPR017066	E3 ubiquitin-protein ligase BOI-like	PANTHER	PTHR42647	SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN	coord: 1..303
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 257..292 score: 9.081335

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr020815.1	Sgr020815.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043067	regulation of programmed cell death
molecular_function	GO:0004842	ubiquitin-protein transferase activity