Sgr020240 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr020240
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionDNA primase
Locationtig00153451: 393939 .. 401271 (+)
RNA-Seq ExpressionSgr020240
SyntenySgr020240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTAGCGAAGCGGTTGAGAATGGAGGAAGCGATATGGTAATTGATGGTATCGAACAGAAGCCGAAGAAAATTGTGCCTGACCGGTTCGATGCTAATTATCTCAAAATTTACTACGGTGAGCCATTTTTGCCTTTCGTTTTTAGAAAATTTTGTATTTTGATCTGGCCTTCTTTCCAGTTGCGATATGAATTGTCCCATGAAATTTAGTTAACTTTTGTCCCTTTATGTTTTTCTGCTACTGAAAGATATGAAATAAAATTGTTGCAGGAAAGCTTTTTCCATACACTGATTTTTTCAAATGGATGTCTTATGGAAATGGTAAGCCGTTCATCTTTGGGAAGAAAAACAAAATATTATCTCCCCCCCCCCCCCCCCCCCCCCCCCCGGGTCTACTTATCTTGGTGCAAGCGAAGGTTTACACAATCGGCAGAGGAAGCTAACTAACATCAAATATGTAACCTTACCTGCTTTATGTGCTTTGTAGATGGCAATCATCCAGGCACTGACCAGTCCTATTTTGGCCGAAGAGAGTTTTCATTCACGTTGGATAATGATATTTATCTACGATTCCAATCTTTCAATAATTTGTCTGAAATGGAAAATTCGATCAAGGAAAAGTGTCCGTTTAAAATTGATATCGGGCCTGTATATAGTGTGGATGTAAGTAAAATTCTTCCTTGTTGGTCTTATCATGAATCTGAATTTCATTCGCCCCGATCAATGTTGTTTGATTTATTTGGGACTTGAAGTTTTTTATTTTAAAATTTTGGAATCATCTCCAATTGAAAGGTATTCCGAGGATGGATATGTAGATTTTTGTATTGCTTAATTGATAATTTTTAACTTTCTAGTTGAAAGATATGAAAGTGAAATAAAGAAAATGAATTATCATGCCACTCTTATGCTTTGAAATTTGACGTCTGATTTGCCTCTGACTTATCTAACTAGGGATGATCATTCCATAGAATGAATTGCAGTGGACCATCTAAGGCTCTTAGAATCAACTAAAGTCTCAAACTCTGCAAGTGCATGAGAATGATTGTCATTGAGTTCATCTCTAACATTCATGTAATTTAAACTAAATACAGACCTGATCTTTCTAGTTCTCTCATAATTATTGATGATTTAGTTACTAGTTTTTGCATAATTTGATTTCTAGAAATCAGGTTTATTTCAAGAAATCAAGGCTTCAGACATTCAGCATACTTGTTTTGAATATTATTTATTTGCATGCTAGCTCTTTTTTGTAATTTTCATCTCTTTCTCTCATTTCAACTCTTCTGCTTCAGCCTGCAAAGAGACACGCATATGCTGGTGATAATGTATTTACTCCAGTGGAGAGAGAGCTGGTTTTTGATATAGTAAGCAGATCAGTTTTCAAAATTCTAATTTAATTTGAAAGTTAATTATGATATCCTTTTCACTGATTATTCAGTGATGTTCCTCCATGTGCAGGATATGACAGATTACGATGATGTTAGATACTGTTGTTCAGGAGCTGATGTTTGCTTGGATTGCTGGCCTTTAATGAGTGCTGCTATCAAAGTCATTGATACTACCCTCCGAGGTAAGAAAATTCATCATCTGTATCTGAAAAGTCATTCAAGAAATGAGTATGATTGCATCCATTCTTACAACCCTCAATTTGAAAAGGAATGAGCATAGAGAAGGTTGCATAGCATTGAATGCTGCTAGCCGTCTCGAGAGAAATCACTTACTTTCTTAGTAGTTTCCCCTCTCCAGCCCCATGCCTAAGCCTATTTCCTTTTTTTTTCTTTTATTGGGAACGAGATGTTCCATATTGAGCTCTTTAATAAAATTACTTCTTTTTAACTTATTCCTAAGTTCTTACTTCAACATATTATTTTATTTGACTAGAAGAGAATTAGGAAGTAGGATATGTCTAAAAGGTGTTATGCATTCTGAAGTATTTAGATTATGAATCACTAGCTTATTATCAGTCCTGCTTTGCTACTTTATCTATTTCAGCCTGTTATATTTGCAATGCTTATCTTTATTTAAATATGTTCACACATGAAGCCTCTCACAACAATTTTTTGAACGTATATTCAAAATTAACCATGATTGGCACTGTGACATTGTACAATGCATATGAGAATTGACTTTTTATTGCAGTTCTATGATTAGATAGCACACAATTTTATATCCAGTCCCATCGGATACACTTAACTTGAAATTTGTGTTGCAGATGACTTTGGATTTAACCACTTACTGTGGGTATTTAGTGGCCGACGTGGTGTTCATTGTTGGGTCTGTGATGGGAAAGCAAGAAGGTAAAGGTAGTAATGTTGAATTTATAGTCATAAAGTCTACTTGTTAAAATTGCCGTCATCACTGCCTCAGGTTGTCTAATGAACAGAGAGCTGCTATCACGGAATATTTCCATTTATATCAGGTTATACTACGTTATAAAATATTGGTGAATTTTAGTGTCTCGAGTTATAAAGTTAAATGTGGTTGATGTGCAGGCTAATGAAAACAGTAATAAGAAAATTTCTCTAACAGGTCCAGTTCTTCATCCATTTCTCGTGTAAGTGGTGCTTAATATTAATATATGATGATTCAAGTCTCATTTTTCTCAGCTAACTTATGTATTTTGTTTCAGGAGATCATACACAGAAGTTCTCAAGGATAGCTTTGAATCAAAATTGCTTTCTAGTCAAAAGATTTTCTCAACTGAGGAGAGATATGAGAAAATCTTAGATATGATTCCTGATGAGTGTAAGTGATCATGTATTCGTCATATGTCAGTTCTGTCATCCTTATGATATGTTAGAAACTAATAGAGTTGCTCTTGTAGCCATTACCTCAGAGCTACGGGGAAGGTGGCAAGAGAATAGGCGACCCTCTAAAAAAGATGTTAATTCTGTAAGATGGGAGCAACTAAAAAATGTGCTGCAATCAGGGAAACATAAGGTACAATCAAACATTTTTTCCTTACTTGAAAGAGATTACAACTTGGAACAGTCAATTGAAGAGGACTTTGTGTTGTTATATTGCAATTTGGAAGAATTTTTGTCAACAAAAATTGAGTTAGAAGTGATTGACCGATTTCCTTTTTCTTTTGTTTATGCTTTCTTTTTTCCAACTCAACTTACCATATAGGAGGGATAGCCATTATACATGGTACTTTCCTTAGGGAAGCAACCATAGATTTCGAGCTCAAGGAAGAAAAAAGAGCTACCTTTTGTCCAAAAGACTTTGCCCAGCTAGAGCTACTTTATGTTCCAAGAACTTCAGTCAATGGTCAAAAGTCCCCTCCCCCTACTTCTCAGGTCAGCTACCTTCTACACCTTTTGTCCTCCCATGGTAACTTCTAAAGCCTAACTGTTATCTTCTACCCTTCTCCCCGAAAATCCAGCCTAATTATCATAGCAGCTTTCTGCCTTGGCCCCACTTTTGTTGTTGATCACTCTTATCCTTCCCCTACAGTGTGTCTCTTACACAGCCCAACCTTTCTTCTGGTAAAGGCCCCTCTATCAACAACTTTGTCCAATCAACAAACCCATCTGCCTCCTCCACCAAAGGTTAAATAAAAAGGGTTTGTTTCTTTCAACTCCAACATTTTCCATCAAAATTTTCTTTGCAAATGGCACTAAACACTCCACCTCCCAGACACTCATCCTTGAACCAGACTTTGATGACTATCTCTCAAGCCCTTTCCTAGAGAACATTTAAGAACATAAAAAAGTCATTTGATATGATAGGAACCATAAAATTGGAATGGAGATTGTATTGAAACTTGAAGAACAAGAAAATACAATAAAATATCCCAGGTAATTCGAAGAATATGGACACCTCCATTTTGGAGCAAGCTCAAGCCCAAACATCTAAATAATGCTCGCCTTCCCTCCCTCTCACTCTAGAATCACCTCTTACTAAAGAACTTCTTAGTTAATGACTAATATTCCCTTTGTATCCTAATAATATTTCTAACATTTATCCTAATAGTATTTCTAACATGATATTTTCTTAGAATCTATATTTTTTATGGCTCCTTTTTGCTCATGGGCTTGCTCCCCCCCCCCCCCCCCCTTATTTTTCATTATTTTTATTTAGGGACTTGGCTTTCCCCCTCTTTTCTTATTTCATATCATCAATGAAATGTTGTTTCTTATTAAAAAAAAGAAAGCTTATTTGATAAGTCATGTGCAGTCCACATAGTTGTAATCTTTATTTTTAGTTGCCTTTTCTCAATCATCGTGCTTTCCTTTTTCCCCTTTCTTTATTTCTTTCCAGTCATGTTTTGGTGTGGCTGCCCCCTAGATATGTGTTGGTGCAACCTTTTCTTTTTTTACTTTTTGAGTCTTGTATGTTGTAGTATTTGGGTAGTGTAGAGTCCAATTTCTAACAACTGGCAACTCTGCTAATTTCGATGATAATTATGACATTGTCCTTTTGTTCAGGCACATGGAATTCGTAGGCATGTTGAAGAGATTGTTTTCTCTTTTACCTACCCTAGGCTCGATTTGGAGGTATGTGTACCTCGCTTTATCTTACCTAGATTTTCCGCCTCTTTGATCAGAATTAGGTTATTATATTCTAGCAACTAAATTCATTGCATATTGGATCTCTAATTGGCCTGCACATGGGATTGTATAAGAAATTTCAAATTTGCTTGTTAAAGCTGGTTTTAATTTTACTCGCTTGATGAGGCAAGTTCTTTATTCTTCAGGTTACTAAACACATGAACCATCTGCTAAAAGCACCATTCTGTGTTCATCCCAAAACAGGTCAGTGCTGGAAATAATTGCTAGATTAGATGAGTAGTAGTGACTTGCAGAAATCCCACTATGGTCTCAATTTTAAATTTACTTCTTTAAGGTTCACTTGATTTGGAATCCTGCAAGTAATTTGATTAGCATGAAAGATGCTTTATATTCACTTCATTTGAAGAGAACAATGCGTTCTACATATTATTAAAGCTTACTTATACTTTTATATTCCAGGTCGTATTTGTGTCCCTATCAATCCTGAACGCTGTGATGAGTTTGATCCTACTACTGTACCAACTATTGCGCAGGTAAAACAGCAAGAAATAAAGGCATAAAGCATAAATGTTATACTTTTGCATACTGATACGCAGCGAGGAAAATGTTTAATTTTCAATTCAATATTTGGGAATTTTTCGTGGTTAGAAATAAATGAGATATCATTTGATGTTAGAAATTTGGAATACGAGGGATTGGACAATGCTTGCTTTTATTTTAAACTAGCTTCTTTATTCCCATTCAATGCATCATTCATATAATGGAAAATATTGGGAAGGGATAATTACTTTCATTCCATTGCAAGTGATTCTAAAAAACATTGCTACTAATATCCTTTGGCATAAAGGAAGGTAAGTTGTGGGAACTTGGCTTTATAGAAACTATATTGTCATTCTCAATCTGTTTGTAAATACAAAACAAACTAAGCTCAACTTTATCTTTTTTTTTTTTTTTAATCCTATATTTATCTTACTACTTGATCAGCATCAAAGCTGTGTTTGGGCTTATTTAAGACCAATTTTTCTGTTTCTGCTGTGACTATCAGAAATTCTTTTGTTGTAAATCAGAGTGATACCTTGCTTTCCTTGTTTTGTCAGCTCTTTTTTGTAACTTTCTTGGCTTGGGGCTTGGCTCTTCTTCCATTCATTTTATAATTTCATACTATCTATGAAATTGCTTCTTATTTTTTAGAAAAAGAAAATTGTCCTTCAACGTTTGGAACTTTCTAAAACTACATAACCATGCTCATTTAATTAGGCAATTGAATATTCTGGCATACATTCCTTGATCAAGTAGCTAGCAGCTCTTTTTTTTCGCATTCTGGCTGAAGAAAGAAGATCAAAATTTGTATAATTAAACCATGATGCATAGTAAATTCTCCATTATGCTTTAAAGTAAATTTGTCTTGTCCAATAAAGTGAATCTGGCAATGATGGATTGCAGCTCCTAGAAGAACTTAATGCTGCAGATATGAAAGTAGATGGTGAGACTGGTATGTTAAGTGCTAACTTCATTGTAGCTTCGAGAGGTTCCTGCTTTATTTGCTTGTGCAGCTAGGGTAGGGATAATTTAATATTAGTGATTAACTAATGCTGTTATAGTCTTTTTACTTCATGACAGATTGGGATAGAACCTCACTTGGGGAATCGATTAGGTTTTTCAGATCATCATTCTTACAGCCACTACTAAAATCATGCAAGGTAATTCTTTTTTAATATATGGAGTAAATCTCTGACCTTCCAAGTTGTAGACCATCGTTCTTGTTGTAGGCTCGAAGTTGTGCTGGGTAATTATCACCGTCTATTAGAAGAACTAGGCTACTAGTTGTGCATCTTCAAACTTTGTTTGCTTTCTTGTGGTTTTATTGACTGTAAAGTGTATAGTCTATTTGTTGGGGTGTGCCTTGATAGGCTCTCTTTTAGTAGGTTTGATAGTTTGTTACTGATAGACCCCAAAAACAGGTCCACCACTACAAAAAGAGATTAATATCACGAAAAGATAACCACAAACACCCCCAGCTCTTCGAGAGGCCGGGGTACTCTCTTCCTTAGAGACTCTCTAAGGCTGTACACAGAATAGATGCCGAATAATGAATCCTAACGAGGGCTCTGCCCTTCCTAAAACCCCTCCACTCACAGCCTAGTACCGTGATCACCTAAAACAAAAATGGGGCCCACTAGCTACACACGCACTAACACATAACTAGCTGTCAGACCCCGTTTTTCCCTTCTTCCTTCGGGTATAAACGAATTGCACGGTCGGTTTGTCCTTCCCATCAGTTACTTCTCAAACATCAATGAAAAGTTCGTTAAAAGTTTGTTTTCTTGTCAGAAAAAAAAAATGTCTTCCTGCATCATCTTCAGGAATTGCCACATTATTATTTAGGTATAGGGAGCATTTTAAGAGTTTTGATGGTTGTTTTGGCTCATTGGATTTAGTCTGAGTTATACATTATAGATATTCTCAATATTAGCCATCTTCTATAAGCGATTCATTCTACTGCTGTCTGGCAAAGGTCGGGAGGCACTAAGGATGGCTAACAAGACTTGTGATCTTTTCTTCTTGCATTCTATGCTTTACACGTTTGAATGACAGACTTTTGTCGTCAAAATGTGACAAAAGAAAGCCAAGCCAAGACAATTGGTCACTCCTTTTATCCACTTGTTTTAAGGCTCAAACACACTTTAAATGCTAGAATGCTGTATATCTTTCGTGAGATCGAGCAAGTTCTTAGCCATGATTCTTGTTTATTGCAGGAGGAGATAGAATCTTCATACAATGCTAAAGTGCAACAGTCGAAAAATTCTCTGAGTTGGTAG

mRNA sequence

ATGATTAGCGAAGCGGTTGAGAATGGAGGAAGCGATATGGTAATTGATGGTATCGAACAGAAGCCGAAGAAAATTGTGCCTGACCGGTTCGATGCTAATTATCTCAAAATTTACTACGGAAAGCTTTTTCCATACACTGATTTTTTCAAATGGATGTCTTATGGAAATGATGGCAATCATCCAGGCACTGACCAGTCCTATTTTGGCCGAAGAGAGTTTTCATTCACGTTGGATAATGATATTTATCTACGATTCCAATCTTTCAATAATTTGTCTGAAATGGAAAATTCGATCAAGGAAAAGTGTCCGTTTAAAATTGATATCGGGCCTGTATATAGTGTGGATCCTGCAAAGAGACACGCATATGCTGGTGATAATGTATTTACTCCAGTGGAGAGAGAGCTGGTTTTTGATATAGATATGACAGATTACGATGATGTTAGATACTGTTGTTCAGGAGCTGATGTTTGCTTGGATTGCTGGCCTTTAATGAGTGCTGCTATCAAAGTCATTGATACTACCCTCCGAGATGACTTTGGATTTAACCACTTACTGTGGGTATTTAGTGGCCGACGTGGTGTTCATTGTTGGGTCTGTGATGGGAAAGCAAGAAGGTTGTCTAATGAACAGAGAGCTGCTATCACGGAATATTTCCATTTATATCAGGCTAATGAAAACAGTAATAAGAAAATTTCTCTAACAGGTCCAGTTCTTCATCCATTTCTCGTGAGATCATACACAGAAGTTCTCAAGGATAGCTTTGAATCAAAATTGCTTTCTAGTCAAAAGATTTTCTCAACTGAGGAGAGATATGAGAAAATCTTAGATATGATTCCTGATGAGTCCATTACCTCAGAGCTACGGGGAAGGTGGCAAGAGAATAGGCGACCCTCTAAAAAAGATGTTAATTCTGTAAGATGGGAGCAACTAAAAAATGTGCTGCAATCAGGGAAACATAAGGCACATGGAATTCGTAGGCATGTTGAAGAGATTGTTTTCTCTTTTACCTACCCTAGGCTCGATTTGGAGGTTACTAAACACATGAACCATCTGCTAAAAGCACCATTCTGTGTTCATCCCAAAACAGGTCGTATTTGTGTCCCTATCAATCCTGAACGCTGTGATGAGTTTGATCCTACTACTGTACCAACTATTGCGCAGCTCCTAGAAGAACTTAATGCTGCAGATATGAAAGTAGATGGTGAGACTGATTGGGATAGAACCTCACTTGGGGAATCGATTAGGTTTTTCAGATCATCATTCTTACAGCCACTACTAAAATCATGCAAGATATTCTCAATATTAGCCATCTTCTATAAGCGATTCATTCTACTGCTGTCTGGCAAAGACTTTTGTCGTCAAAATGTGACAAAAGAAAGCCAAGCCAAGACAATTGGTCACTCCTTTTATCCACTTGTTTTAAGGCTCAAACACACTTTAAATGCTAGAATGCTGTATATCTTTCGTGAGATCGAGCAAGTTCTTAGCCATGATTCTTGTTTATTGCAGGAGGAGATAGAATCTTCATACAATGCTAAAGTGCAACAGTCGAAAAATTCTCTGAGTTGGTAG

Coding sequence (CDS)

ATGATTAGCGAAGCGGTTGAGAATGGAGGAAGCGATATGGTAATTGATGGTATCGAACAGAAGCCGAAGAAAATTGTGCCTGACCGGTTCGATGCTAATTATCTCAAAATTTACTACGGAAAGCTTTTTCCATACACTGATTTTTTCAAATGGATGTCTTATGGAAATGATGGCAATCATCCAGGCACTGACCAGTCCTATTTTGGCCGAAGAGAGTTTTCATTCACGTTGGATAATGATATTTATCTACGATTCCAATCTTTCAATAATTTGTCTGAAATGGAAAATTCGATCAAGGAAAAGTGTCCGTTTAAAATTGATATCGGGCCTGTATATAGTGTGGATCCTGCAAAGAGACACGCATATGCTGGTGATAATGTATTTACTCCAGTGGAGAGAGAGCTGGTTTTTGATATAGATATGACAGATTACGATGATGTTAGATACTGTTGTTCAGGAGCTGATGTTTGCTTGGATTGCTGGCCTTTAATGAGTGCTGCTATCAAAGTCATTGATACTACCCTCCGAGATGACTTTGGATTTAACCACTTACTGTGGGTATTTAGTGGCCGACGTGGTGTTCATTGTTGGGTCTGTGATGGGAAAGCAAGAAGGTTGTCTAATGAACAGAGAGCTGCTATCACGGAATATTTCCATTTATATCAGGCTAATGAAAACAGTAATAAGAAAATTTCTCTAACAGGTCCAGTTCTTCATCCATTTCTCGTGAGATCATACACAGAAGTTCTCAAGGATAGCTTTGAATCAAAATTGCTTTCTAGTCAAAAGATTTTCTCAACTGAGGAGAGATATGAGAAAATCTTAGATATGATTCCTGATGAGTCCATTACCTCAGAGCTACGGGGAAGGTGGCAAGAGAATAGGCGACCCTCTAAAAAAGATGTTAATTCTGTAAGATGGGAGCAACTAAAAAATGTGCTGCAATCAGGGAAACATAAGGCACATGGAATTCGTAGGCATGTTGAAGAGATTGTTTTCTCTTTTACCTACCCTAGGCTCGATTTGGAGGTTACTAAACACATGAACCATCTGCTAAAAGCACCATTCTGTGTTCATCCCAAAACAGGTCGTATTTGTGTCCCTATCAATCCTGAACGCTGTGATGAGTTTGATCCTACTACTGTACCAACTATTGCGCAGCTCCTAGAAGAACTTAATGCTGCAGATATGAAAGTAGATGGTGAGACTGATTGGGATAGAACCTCACTTGGGGAATCGATTAGGTTTTTCAGATCATCATTCTTACAGCCACTACTAAAATCATGCAAGATATTCTCAATATTAGCCATCTTCTATAAGCGATTCATTCTACTGCTGTCTGGCAAAGACTTTTGTCGTCAAAATGTGACAAAAGAAAGCCAAGCCAAGACAATTGGTCACTCCTTTTATCCACTTGTTTTAAGGCTCAAACACACTTTAAATGCTAGAATGCTGTATATCTTTCGTGAGATCGAGCAAGTTCTTAGCCATGATTCTTGTTTATTGCAGGAGGAGATAGAATCTTCATACAATGCTAAAGTGCAACAGTCGAAAAATTCTCTGAGTTGGTAG

Protein sequence

MISEAVENGGSDMVIDGIEQKPKKIVPDRFDANYLKIYYGKLFPYTDFFKWMSYGNDGNHPGTDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMENSIKEKCPFKIDIGPVYSVDPAKRHAYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMSAAIKVIDTTLRDDFGFNHLLWVFSGRRGVHCWVCDGKARRLSNEQRAAITEYFHLYQANENSNKKISLTGPVLHPFLVRSYTEVLKDSFESKLLSSQKIFSTEERYEKILDMIPDESITSELRGRWQENRRPSKKDVNSVRWEQLKNVLQSGKHKAHGIRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPINPERCDEFDPTTVPTIAQLLEELNAADMKVDGETDWDRTSLGESIRFFRSSFLQPLLKSCKIFSILAIFYKRFILLLSGKDFCRQNVTKESQAKTIGHSFYPLVLRLKHTLNARMLYIFREIEQVLSHDSCLLQEEIESSYNAKVQQSKNSLSW
Homology
BLAST of Sgr020240 vs. NCBI nr
Match: XP_022141835.1 (DNA primase small subunit-like [Momordica charantia] >XP_022141836.1 DNA primase small subunit-like [Momordica charantia] >XP_022141837.1 DNA primase small subunit-like [Momordica charantia] >XP_022141838.1 DNA primase small subunit-like [Momordica charantia])

HSP 1 Score: 832.4 bits (2149), Expect = 2.1e-237
Identity = 415/536 (77.43%), Postives = 431/536 (80.41%), Query Frame = 0

Query: 1   MISEAVENGGSDMVIDGIEQKPKKIVPDRFDANYLKIYYGKLFPYTDFFKWMSYGNDGNH 60
           MI+EAVENGG DM IDG EQKP+K+VPD FD NYLKIYYGKLFPYTDFFKWMSYGNDG H
Sbjct: 1   MITEAVENGGGDMEIDGFEQKPEKVVPDGFDVNYLKIYYGKLFPYTDFFKWMSYGNDGKH 60

Query: 61  PGTDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PG+DQSYFGRREFSFTL+NDIYLR+QSFNNLSEMENSIKEKCPFKIDIGPVYSVD AKRH
Sbjct: 61  PGSDQSYFGRREFSFTLENDIYLRYQSFNNLSEMENSIKEKCPFKIDIGPVYSVDLAKRH 120

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMSAAIKVIDTTLRDDFG 180
           AYAGDNVFTPVEREL+FDIDMTDYDDVRYCCSGADVCLDCWPLM+AAIKVIDTTLRDDFG
Sbjct: 121 AYAGDNVFTPVERELIFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180

Query: 181 FNHLLWVFSGRRGVHCWVCDGKARRLSNEQRAAITEYFHLYQANENSNKKISLTGPVLHP 240
           FNH+LWVFSGRRGVHCWVCDGKARRLSNEQRAAI E+FHLYQ NENS KKISLTGPVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEHFHLYQGNENSRKKISLTGPVLHP 240

Query: 241 FLVRSYTEVLKDSFESKLLSSQKIFSTEERYEKILDMIPDESITSELRGRWQENRRPSKK 300
           FL RSYTEVLKDSFES LL SQKIFSTEERYEKILDMIPDES+TSELRGRWQENR+PSKK
Sbjct: 241 FLARSYTEVLKDSFESTLLCSQKIFSTEERYEKILDMIPDESVTSELRGRWQENRQPSKK 300

Query: 301 DVNSVRWEQLKNVLQSGKHKAHGIRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVN VRWEQLK++LQS KHKA G+RRHVEEIVFSFTYPRLDLEVTKH NHLLKAPFCVHP
Sbjct: 301 DVNYVRWEQLKSMLQSVKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHTNHLLKAPFCVHP 360

Query: 361 KTGRICVPINPERCDEFDPTTVPTIAQLLEELNAADM-------------KVDGETDWDR 420
           KTGRICVPINPE CDEFDPTTVPT+AQLLEELNAADM             KVDGETDWDR
Sbjct: 361 KTGRICVPINPENCDEFDPTTVPTLAQLLEELNAADMNVDGEYSQEKKRQKVDGETDWDR 420

Query: 421 TSLGESIRFFRSSFLQPLLKSCKIFSILAIFYKRFILLLSGKDFCRQNVTKESQAKTIGH 480
           TSLGESIRFFRSSFLQPLLKSCK                                     
Sbjct: 421 TSLGESIRFFRSSFLQPLLKSCK------------------------------------- 463

Query: 481 SFYPLVLRLKHTLNARMLYIFREIEQVLSHDSCLLQEEIESSYNAKVQQSKNSLSW 524
                                               EEIESSYNAKVQQSKN LSW
Sbjct: 481 ------------------------------------EEIESSYNAKVQQSKNPLSW 463

BLAST of Sgr020240 vs. NCBI nr
Match: XP_004141619.1 (DNA primase small subunit [Cucumis sativus] >XP_031742330.1 DNA primase small subunit [Cucumis sativus] >KGN52619.1 hypothetical protein Csa_007851 [Cucumis sativus])

HSP 1 Score: 831.2 bits (2146), Expect = 4.8e-237
Identity = 412/523 (78.78%), Postives = 428/523 (81.84%), Query Frame = 0

Query: 1   MISEAVENGGSDMVIDGIEQKPKKIVPDRFDANYLKIYYGKLFPYTDFFKWMSYGNDGNH 60
           M++ AVENGGSDMVIDG EQKPK  V D FDANYLKIYYGKLFPY DFFKWMSYGNDG H
Sbjct: 1   MVTAAVENGGSDMVIDGNEQKPKNTVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 60

Query: 61  PGTDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PG+DQSYFGRREFSFTLDNDIYLRFQSFNNLSEME SIKEKCPFKIDIGPVYSVDPAKRH
Sbjct: 61  PGSDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMEISIKEKCPFKIDIGPVYSVDPAKRH 120

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMSAAIKVIDTTLRDDFG 180
           AYA DNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLM+AAIKVI TTLRDDFG
Sbjct: 121 AYADDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIHTTLRDDFG 180

Query: 181 FNHLLWVFSGRRGVHCWVCDGKARRLSNEQRAAITEYFHLYQANENSNKKISLTGPVLHP 240
           FNH+LWVFSGRRGVHCWVCDGKARRLSNEQRAAI EYFHLYQ NENS KKISLTGPVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 240

Query: 241 FLVRSYTEVLKDSFESKLLSSQKIFSTEERYEKILDMIPDESITSELRGRWQENRRPSKK 300
           FLVRSYTEVLKDSFES+LL SQKIFSTEERYEKILDMIPDESITS+LRGRWQ+NRR SK 
Sbjct: 241 FLVRSYTEVLKDSFESQLLCSQKIFSTEERYEKILDMIPDESITSDLRGRWQDNRRSSKN 300

Query: 301 DVNSVRWEQLKNVLQSGKHKAHGIRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVNS+RWEQLK++LQSGKHKA G+RRHVEEIVFSFTYPRLD EVTKHMNHLLKAPFCVHP
Sbjct: 301 DVNSLRWEQLKSLLQSGKHKAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHP 360

Query: 361 KTGRICVPINPERCDEFDPTTVPTIAQLLEELNAADMKVDGETDWDRTSLGESIRFFRSS 420
           KTGRICVPI+PE CDEFDPTTVPT+AQLLEELNAADM+VD E DWDRTSLGESIRFFRS+
Sbjct: 361 KTGRICVPIDPEHCDEFDPTTVPTVAQLLEELNAADMEVDSENDWDRTSLGESIRFFRSA 420

Query: 421 FLQPLLKSCKIFSILAIFYKRFILLLSGKDFCRQNVTKESQAKTIGHSFYPLVLRLKHTL 480
           FLQPLLKSCK                                                  
Sbjct: 421 FLQPLLKSCK-------------------------------------------------- 450

Query: 481 NARMLYIFREIEQVLSHDSCLLQEEIESSYNAKVQQSKNSLSW 524
                                  EEIESSYNAKVQQSKNSLSW
Sbjct: 481 -----------------------EEIESSYNAKVQQSKNSLSW 450

BLAST of Sgr020240 vs. NCBI nr
Match: XP_023535939.1 (DNA primase small subunit [Cucurbita pepo subsp. pepo])

HSP 1 Score: 826.2 bits (2133), Expect = 1.5e-235
Identity = 409/523 (78.20%), Postives = 426/523 (81.45%), Query Frame = 0

Query: 1   MISEAVENGGSDMVIDGIEQKPKKIVPDRFDANYLKIYYGKLFPYTDFFKWMSYGNDGNH 60
           M+ EA ENGGSDMVIDG EQKPK IV + FDANYLKIYYGKLFP+ DFFKWMSYGNDG H
Sbjct: 1   MVHEAAENGGSDMVIDGNEQKPKNIVSNVFDANYLKIYYGKLFPFADFFKWMSYGNDGKH 60

Query: 61  PGTDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PG+D SY GRREFSFTLDNDIYLRFQSFN+LSEME+S KEKCPFKIDIGPVYSVDPAKRH
Sbjct: 61  PGSDHSYIGRREFSFTLDNDIYLRFQSFNSLSEMESSTKEKCPFKIDIGPVYSVDPAKRH 120

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMSAAIKVIDTTLRDDFG 180
           AYAG NVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLM+AAIKVIDTTLRDDFG
Sbjct: 121 AYAGANVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180

Query: 181 FNHLLWVFSGRRGVHCWVCDGKARRLSNEQRAAITEYFHLYQANENSNKKISLTGPVLHP 240
           FNH+LWVFSGRRGVHCWVCDGKARRLSNEQRAAI +YFHLYQ NENS KKISLTGPVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIADYFHLYQGNENSRKKISLTGPVLHP 240

Query: 241 FLVRSYTEVLKDSFESKLLSSQKIFSTEERYEKILDMIPDESITSELRGRWQENRRPSKK 300
           FL RSY EVLKDSFESKLL SQKIFSTEERYEKILDMI DESITSELRGRWQ+NRRPSK 
Sbjct: 241 FLARSYIEVLKDSFESKLLCSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKN 300

Query: 301 DVNSVRWEQLKNVLQSGKHKAHGIRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVN VRWEQLKN+LQSGKHK+ G+RRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP
Sbjct: 301 DVNLVRWEQLKNMLQSGKHKSQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360

Query: 361 KTGRICVPINPERCDEFDPTTVPTIAQLLEELNAADMKVDGETDWDRTSLGESIRFFRSS 420
           KTGRICVPI+PERCDEFDPTTVPT+AQLLEELNAADM+VD ETDWDRTSLGESIRFFRS+
Sbjct: 361 KTGRICVPIDPERCDEFDPTTVPTVAQLLEELNAADMEVDSETDWDRTSLGESIRFFRSA 420

Query: 421 FLQPLLKSCKIFSILAIFYKRFILLLSGKDFCRQNVTKESQAKTIGHSFYPLVLRLKHTL 480
           FLQPLLKSCK                                                  
Sbjct: 421 FLQPLLKSCK-------------------------------------------------- 450

Query: 481 NARMLYIFREIEQVLSHDSCLLQEEIESSYNAKVQQSKNSLSW 524
                                  EEIESSYNAKVQQSKN LSW
Sbjct: 481 -----------------------EEIESSYNAKVQQSKNPLSW 450

BLAST of Sgr020240 vs. NCBI nr
Match: KAG6592333.1 (DNA primase small subunit, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 824.7 bits (2129), Expect = 4.5e-235
Identity = 407/523 (77.82%), Postives = 426/523 (81.45%), Query Frame = 0

Query: 1   MISEAVENGGSDMVIDGIEQKPKKIVPDRFDANYLKIYYGKLFPYTDFFKWMSYGNDGNH 60
           M+ EA ENGGSDMVIDG EQKPK IV + FDANYLKIYYGKLFP+ DFFKWMSYGNDG H
Sbjct: 1   MVHEAAENGGSDMVIDGNEQKPKNIVSNGFDANYLKIYYGKLFPFADFFKWMSYGNDGKH 60

Query: 61  PGTDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PG+D SY GRREFSFTLDNDIYLRFQSFN+LSEME+S KEKCPFKIDIGPVYSVDPAKRH
Sbjct: 61  PGSDHSYIGRREFSFTLDNDIYLRFQSFNSLSEMESSTKEKCPFKIDIGPVYSVDPAKRH 120

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMSAAIKVIDTTLRDDFG 180
           AYAG NVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLM+AAIKVID+TLRDDFG
Sbjct: 121 AYAGANVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDSTLRDDFG 180

Query: 181 FNHLLWVFSGRRGVHCWVCDGKARRLSNEQRAAITEYFHLYQANENSNKKISLTGPVLHP 240
           FNH+LWVFSGRRGVHCWVCDGKARRLSNEQRAAI +YFHLYQ NENS KKISLTGPVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIADYFHLYQGNENSRKKISLTGPVLHP 240

Query: 241 FLVRSYTEVLKDSFESKLLSSQKIFSTEERYEKILDMIPDESITSELRGRWQENRRPSKK 300
           FL RSY EVLKDSFESKLL SQKIFSTEERYEK+LDMI DESITSELRGRWQ+NRRPSK 
Sbjct: 241 FLARSYIEVLKDSFESKLLCSQKIFSTEERYEKVLDMIHDESITSELRGRWQDNRRPSKN 300

Query: 301 DVNSVRWEQLKNVLQSGKHKAHGIRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVN VRWEQLKN+LQSGKHK+ G+RRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP
Sbjct: 301 DVNLVRWEQLKNMLQSGKHKSQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360

Query: 361 KTGRICVPINPERCDEFDPTTVPTIAQLLEELNAADMKVDGETDWDRTSLGESIRFFRSS 420
           KTGRICVPI+PERCDEFDPTTVPT+AQLLEELNAADM+VD ETDWDRTSLGESIRFFRS+
Sbjct: 361 KTGRICVPIDPERCDEFDPTTVPTVAQLLEELNAADMEVDSETDWDRTSLGESIRFFRSA 420

Query: 421 FLQPLLKSCKIFSILAIFYKRFILLLSGKDFCRQNVTKESQAKTIGHSFYPLVLRLKHTL 480
           FLQPLLKSCK                                                  
Sbjct: 421 FLQPLLKSCK-------------------------------------------------- 450

Query: 481 NARMLYIFREIEQVLSHDSCLLQEEIESSYNAKVQQSKNSLSW 524
                                  EEIESSYNAKVQQSKN LSW
Sbjct: 481 -----------------------EEIESSYNAKVQQSKNPLSW 450

BLAST of Sgr020240 vs. NCBI nr
Match: XP_022925368.1 (DNA primase small subunit [Cucurbita moschata])

HSP 1 Score: 824.3 bits (2128), Expect = 5.9e-235
Identity = 408/523 (78.01%), Postives = 426/523 (81.45%), Query Frame = 0

Query: 1   MISEAVENGGSDMVIDGIEQKPKKIVPDRFDANYLKIYYGKLFPYTDFFKWMSYGNDGNH 60
           M+ EA ENGGSDMVIDG EQKPK IV + FDANYL+IYYGKLFP+ DFFKWMSYGNDG H
Sbjct: 1   MVHEAAENGGSDMVIDGNEQKPKNIVSNGFDANYLEIYYGKLFPFADFFKWMSYGNDGKH 60

Query: 61  PGTDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PG+D SY GRREFSFTLDNDIYLRFQSFN+LSEME+S KEKCPFKIDIGPVYSVDPAKRH
Sbjct: 61  PGSDHSYIGRREFSFTLDNDIYLRFQSFNSLSEMESSTKEKCPFKIDIGPVYSVDPAKRH 120

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMSAAIKVIDTTLRDDFG 180
           AYAG NVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLM+AAIKVIDTTLRDDFG
Sbjct: 121 AYAGANVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180

Query: 181 FNHLLWVFSGRRGVHCWVCDGKARRLSNEQRAAITEYFHLYQANENSNKKISLTGPVLHP 240
           FNH+LWVFSGRRGVHCWVCDGKARRLSNEQRAAI +YFHLYQ NENS KKISLTGPVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIADYFHLYQGNENSRKKISLTGPVLHP 240

Query: 241 FLVRSYTEVLKDSFESKLLSSQKIFSTEERYEKILDMIPDESITSELRGRWQENRRPSKK 300
           FL RSY EVLKDSFESKLL SQKIFSTEERYEKILDMI DESITSELRGRWQ+NRRPSK 
Sbjct: 241 FLARSYIEVLKDSFESKLLCSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKN 300

Query: 301 DVNSVRWEQLKNVLQSGKHKAHGIRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVN VRWEQLKN+LQSGKHK+ G+RRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP
Sbjct: 301 DVNLVRWEQLKNMLQSGKHKSPGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360

Query: 361 KTGRICVPINPERCDEFDPTTVPTIAQLLEELNAADMKVDGETDWDRTSLGESIRFFRSS 420
           KTGRICVPI+PERCDEFDPTTVPT+AQLLEELNAADM+VD ETDWDRTSLGESIRFFRS+
Sbjct: 361 KTGRICVPIDPERCDEFDPTTVPTVAQLLEELNAADMEVDSETDWDRTSLGESIRFFRSA 420

Query: 421 FLQPLLKSCKIFSILAIFYKRFILLLSGKDFCRQNVTKESQAKTIGHSFYPLVLRLKHTL 480
           FLQPLLKSCK                                                  
Sbjct: 421 FLQPLLKSCK-------------------------------------------------- 450

Query: 481 NARMLYIFREIEQVLSHDSCLLQEEIESSYNAKVQQSKNSLSW 524
                                  EEIESSYNAKVQQSKN LSW
Sbjct: 481 -----------------------EEIESSYNAKVQQSKNPLSW 450

BLAST of Sgr020240 vs. ExPASy Swiss-Prot
Match: P49642 (DNA primase small subunit OS=Homo sapiens OX=9606 GN=PRIM1 PE=1 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 3.4e-92
Identity = 187/413 (45.28%), Postives = 259/413 (62.71%), Query Frame = 0

Query: 35  LKIYYGKLFPYTDFFKWMSYGNDGNHPGTDQSYFGRREFSFTLDNDIYLRFQSFNNLSEM 94
           LK+YY +LFPY+ +++W++YG      G  ++YF  REFSFTL +DIY+R+QSFNN S++
Sbjct: 13  LKLYYRRLFPYSQYYRWLNYG------GVIKNYFQHREFSFTLKDDIYIRYQSFNNQSDL 72

Query: 95  ENSIKEKCPFKIDIGPVYSVDPAKRHAYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGA 154
           E  +++  P+KIDIG VYS  P  +H       F   E+ELVFDIDMTDYDDVR CCS A
Sbjct: 73  EKEMQKMNPYKIDIGAVYSHRP-NQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSA 132

Query: 155 DVCLDCWPLMSAAIKVIDTTLRDDFGFNHLLWVFSGRRGVHCWVCDGKARRLSNEQRAAI 214
           D+C  CW LM+ AI++ID  L++DFGF H LWV+SGRRGVHCWVCD   R+LS+  R+ I
Sbjct: 133 DICPKCWTLMTMAIRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGI 192

Query: 215 TEYFHLYQANENSNKKISLTGPVLHPFLVRSYTEVLKDSFESKLLSSQKIFSTEERYEKI 274
            EY  L +  ++  KK+ L+  + HPF +R    ++K  FE   L +Q I   +E ++KI
Sbjct: 193 VEYLSLVKGGQDVKKKVHLSEKI-HPF-IRKSINIIKKYFEEYALVNQDILENKESWDKI 252

Query: 275 LDMIPDESITSELRGRWQENRRPSKKDVNSV-RWEQLKNVLQSGKHKAHGIRRH---VEE 334
           L ++P E+I  EL+  +Q       K  NS+ RWE LK V    ++     +       E
Sbjct: 253 LALVP-ETIHDELQQSFQ-------KSHNSLQRWEHLKKVASRYQNNIKNDKYGPWLEWE 312

Query: 335 IVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPINPERCDEFDPTTVPTIAQLLE 394
           I+  + +PRLD+ V+K +NHLLK+PF VHPKTGRI VPI+ ++ D+FDP TVPTI+ +  
Sbjct: 313 IMLQYCFPRLDINVSKGINHLLKSPFSVHPKTGRISVPIDLQKVDQFDPFTVPTISFICR 372

Query: 395 EL------------NAADMKVDGET-DWDRTSLGESIRFFRSSFLQPLLKSCK 431
           EL            N A+  V   T D+ +TSL   ++ F   FL+ L KS K
Sbjct: 373 ELDAISTNEEEKEENEAESDVKHRTRDYKKTSLAPYVKVF-EHFLENLDKSRK 407

BLAST of Sgr020240 vs. ExPASy Swiss-Prot
Match: P20664 (DNA primase small subunit OS=Mus musculus OX=10090 GN=Prim1 PE=1 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 5.7e-92
Identity = 186/409 (45.48%), Postives = 255/409 (62.35%), Query Frame = 0

Query: 35  LKIYYGKLFPYTDFFKWMSYGNDGNHPGTDQSYFGRREFSFTLDNDIYLRFQSFNNLSEM 94
           LK+YY +LFPY  +++W++YG      G  ++YF  REFSFTL +DIY+R+QSFNN SE+
Sbjct: 13  LKLYYRRLFPYAQYYRWLNYG------GVTKNYFQHREFSFTLKDDIYIRYQSFNNQSEL 72

Query: 95  ENSIKEKCPFKIDIGPVYSVDPAKRHAYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGA 154
           E  +++  P+KIDIG VYS  P  +H       F   E+ELVFDIDMTDYDDVR CCS A
Sbjct: 73  EKEMQKMNPYKIDIGAVYSHRP-NQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSA 132

Query: 155 DVCLDCWPLMSAAIKVIDTTLRDDFGFNHLLWVFSGRRGVHCWVCDGKARRLSNEQRAAI 214
           D+C  CW LM+ A+++ID  L++DFGF H LWV+SGRRGVHCWVCD   R+LS+  R+ I
Sbjct: 133 DICSKCWTLMTMAMRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGI 192

Query: 215 TEYFHLYQANENSNKKISLTGPVLHPFLVRSYTEVLKDSFESKLLSSQKIFSTEERYEKI 274
            EY  L +  ++  KK+ L   V HPF VR    ++K  FE   L  Q I   +E ++KI
Sbjct: 193 VEYLSLVKGGQDVKKKVHLNEKV-HPF-VRKSINIIKKYFEEYALVGQDILENKENWDKI 252

Query: 275 LDMIPDESITSELRGRWQENRRPSKKDVNSVRWEQLKNVLQSGKH-KAHGIRRHVE-EIV 334
           L ++P E+I  EL+  +Q      K   +  RWE L+ V  S ++ K       +E E++
Sbjct: 253 LALVP-ETIHDELQRGFQ------KFHSSPQRWEHLRKVANSSQNMKNDKCGPWLEWEVM 312

Query: 335 FSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPINPERCDEFDPTTVPTIAQLLEEL 394
             + +PRLD+ V+K +NHLLK+PF VHPKTGRI VPI+  + D+FDP TVPTI+ +  EL
Sbjct: 313 LQYCFPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPIDFHKVDQFDPFTVPTISAICREL 372

Query: 395 NAADM----KVDGETD-------WDRTSLGESIRFFRSSFLQPLLKSCK 431
           +        K + E D       + +TSL   ++ F   FL+ L KS K
Sbjct: 373 DMVSTHEKEKEENEADSKHRVRGYKKTSLAPYVKVF-EQFLENLDKSRK 404

BLAST of Sgr020240 vs. ExPASy Swiss-Prot
Match: Q24317 (DNA primase small subunit OS=Drosophila melanogaster OX=7227 GN=Prim1 PE=1 SV=2)

HSP 1 Score: 316.2 bits (809), Expect = 6.8e-85
Identity = 171/417 (41.01%), Postives = 238/417 (57.07%), Query Frame = 0

Query: 35  LKIYYGKLFPYTDFFKWMSYGNDGNHPGTDQSYFGRREFSFTLDNDIYLRFQSFNNLSEM 94
           L +YY +LFP+  F++W+SYG+      ++ + F  RE SFTL +DIY+R+  F+  +E+
Sbjct: 32  LPVYYRRLFPHLPFYRWLSYGS------SEDAIFSNREISFTLQDDIYIRYLCFDTQAEL 91

Query: 95  ENSIKEKCPFKIDIGPVYSVDPAKRHAYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGA 154
           E  I  + P KIDIGPV    P    +  G    TPV+RELVFDIDMTDYD+VR CCSGA
Sbjct: 92  EKEICSRNPIKIDIGPVMHSKPKNHRSIPGG--LTPVQRELVFDIDMTDYDEVRTCCSGA 151

Query: 155 DVCLDCWPLMSAAIKVIDTTLRDDFGFNHLLWVFSGRRGVHCWVCDGKARRLSNEQRAAI 214
            VCL CW  M  A +V+D  LR+DFGF H++W+FSGRRG+HCWVCD +AR L    R A+
Sbjct: 152 GVCLKCWKFMVLAARVLDVALREDFGFEHIIWIFSGRRGIHCWVCDYQARHLDGRGRYAV 211

Query: 215 TEYFHL--YQANENSNKKISLTGPVLHPFLVRSYTEVLKDSFESKLLSSQKIFSTEERYE 274
            EY ++  Y +    N      G   H  L R+  ++++  FE  +L  Q +F T +   
Sbjct: 212 AEYLNIITYASFAGGNSPRCSMGDRPHHSLKRA-LKIVEPMFEEIVLEDQNLFGTPKGVT 271

Query: 275 KILDMIPDESITSELRGRWQENRRPSKKDVNSVRWEQLKNVLQSGKHK-----AHGIRRH 334
           K+L+M+ D++   EL    Q+N        + + WE       S +       +  ++  
Sbjct: 272 KLLNMVHDDAARGELESYMQKNLEDGAH--SRLVWESFIKYANSMRTSTTSAWSRKLKNI 331

Query: 335 VEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPINPERCDEFDPTTVPTIAQ 394
           V EI     YPRLD+ VT+  NHLLKAPFC+HP TG++CVP +     +FDPTTVPTI Q
Sbjct: 332 VAEIQLGLLYPRLDINVTRGFNHLLKAPFCIHPATGKVCVPFSVSAVAKFDPTTVPTITQ 391

Query: 395 LLEELNAADMKVDGE----------TDWDRTSLGESIRFFRSSFLQPLLKSCKIFSI 435
           LL E+NA D K               D  +TS+ + +  F   FL+ L +S K  S+
Sbjct: 392 LLHEINAFDDKSKSYMEAPEDKSRIKDHKKTSMFKGVVVF-EEFLRKLERSQKSASL 436

BLAST of Sgr020240 vs. ExPASy Swiss-Prot
Match: O14215 (DNA primase small subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pri1 PE=1 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 1.4e-82
Identity = 162/417 (38.85%), Postives = 240/417 (57.55%), Query Frame = 0

Query: 2   ISEAVENGGSDMVIDGIEQKPKKIVPDRFDANYLKIYYGKLFPYTDFFKWMSYGNDGNHP 61
           + E + NG     +DG +Q          D+  +  YY  LFP+   F+W+      NH 
Sbjct: 13  LDEIIANG----TLDGAKQ-------GAVDSETMIQYYRHLFPWKYLFQWL------NHG 72

Query: 62  GTDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMENSIKEKCPFKIDIGPVYSVDPAKRHA 121
               + F  REF+FTL ND Y+R+ SF+N  E++      CP + ++GPVYS +P  R  
Sbjct: 73  PVVTNDFAHREFAFTLPNDAYIRYLSFSNWEELKKEALNLCPSRFEVGPVYSANPRDRKT 132

Query: 122 YAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMSAAIKVIDTTLRDDFGF 181
               + F P+++ELVFDIDMTDYDDVR CCS  ++C  CWP ++ A++V+D    +DFGF
Sbjct: 133 IR-KSTFHPLKKELVFDIDMTDYDDVRTCCSKTNICEKCWPFITIAVQVLDICFHEDFGF 192

Query: 182 NHLLWVFSGRRGVHCWVCDGKARRLSNEQRAAITEYFHLYQANENSNKKI--SLTGPVLH 241
            H+LWV+SGRRG+H W+CD  A  L +  R  I  Y  +   N     ++  +L  P LH
Sbjct: 193 KHILWVYSGRRGIHAWICDEIACSLDDRSRRMIASYLQVVVGNPQGGVRLINNLKRP-LH 252

Query: 242 PFLVRSYTEVLKDSFESKLLSSQKIFSTEERYEKILDMIPDESITSELRGRWQENRRPSK 301
           P L RS   +LK +F   +L  Q  ++++E  E +L ++PD+ + S LR +W+ +   S 
Sbjct: 253 PHLTRS-LNILKSAFVKIVLEDQDPWASKEGAENLLKLLPDKDLASALRKKWEVDPERSS 312

Query: 302 KDVNSVRWEQLKNVLQSGKHKAHG---IRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPF 361
           K+    +W  +  VL SG   +     I    ++IV ++ YPRLD+EV++H+NHLLK+PF
Sbjct: 313 KN----KWSDIDTVLASGSIASISPSVIAIAKQDIVLTYLYPRLDVEVSRHLNHLLKSPF 372

Query: 362 CVHPKTGRICVPINPERCDEFDPTTVPTIAQLLEELNAADMKVDGETDWDRTSLGES 414
           CVHP T R+CVPI+ ER D F+P  VPT+  LL+EL+      +G      T+  E+
Sbjct: 373 CVHPGTSRVCVPIDIERMDSFNPLKVPTVNDLLQELDKNSQNDNGHGPTMETNTTEN 405

BLAST of Sgr020240 vs. ExPASy Swiss-Prot
Match: Q7KQM1 (DNA primase small subunit OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=PF14_0366 PE=3 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 6.0e-73
Identity = 154/424 (36.32%), Postives = 235/424 (55.42%), Query Frame = 0

Query: 33  NYLKIYYGKLFPYTDFFKWMSYGNDGNHPGT---DQSYFGRREFSFTL------DNDIYL 92
           N L  YY  L P  D + W++Y ND     T   D  +F +REFSFT         +IY+
Sbjct: 17  NDLIFYYRSLCPINDLYNWLNYKNDIKGKYTKLNDPHFFSKREFSFTCKKSDQGKEEIYI 76

Query: 93  RFQSFNNLSEMENS-IKEKCPFKIDIGPVYSVDPAKRHAYAGDNVFTPVERELVFDIDMT 152
           R+ SF+N  E +N  + +  P K DIG +Y+  P  +    GD +F PV++EL+FDIDM 
Sbjct: 77  RWLSFSNPEEFKNKLLSDLVPIKFDIGAIYNF-PVSQKDQKGD-IFLPVQKELIFDIDMN 136

Query: 153 DYDDVRYCCSGADVCLDCWPLMSAAIKVIDTTLRDDFGFNHLLWVFSGRRGVHCWVCDGK 212
           DYDD+R CC+   VC  CW  ++ AI ++DT LR+DF F H+LWV+SGRRG+HCWV D  
Sbjct: 137 DYDDIRTCCTDKKVCKLCWKFLTVAIVLLDTALREDFSFEHILWVYSGRRGIHCWVADES 196

Query: 213 ARRLSNEQRAAITEYFHLYQANENSNKKISLTGPVLHPFLVRSYTEVLKDSFESKLLSSQ 272
            R  + + RAA+ +Y ++   ++   KK+S+ G   +P   R++    K  +   L+  Q
Sbjct: 197 CRYYTTDARAALADYLNILSGSDTKKKKVSIWGKDKYPMFERAFDICYK--YFDVLMEEQ 256

Query: 273 KIFST-EERYEKILDMIPDES--ITSELRG-------RWQENRRPSKKDVNSVRWEQLKN 332
             F       +K++D +P  S  +T  L+         +  N   + +++   ++  + N
Sbjct: 257 DFFKKGSPHVQKLIDYLPYASGKVTDPLKAMKLNELKEYINNNNFNSREIFE-KFSSIYN 316

Query: 333 VLQSGKHKAH-------GIRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRI 392
            L    +           +   V+EIVF FTYPRLD+ V+K +NHLLK+PFC+H  TGR+
Sbjct: 317 FLTPSNYFKRKNVSGNINMPSFVKEIVFHFTYPRLDINVSKEINHLLKSPFCIHNSTGRV 376

Query: 393 CVPINPERCDEFDPTTVPTIAQLLEELNAADMKVDGETDWDRTSLGESIRFFRSSFLQPL 430
           CVP++ +  + F+P +VPT+  L E+ +  D K       +RTSL   I +FR  F++ +
Sbjct: 377 CVPLDIKNINNFNPQSVPTLKLLREQFD--DPKNSHIEAENRTSLKPYIDYFRRHFIENI 433

BLAST of Sgr020240 vs. ExPASy TrEMBL
Match: A0A6J1CKZ3 (DNA primase OS=Momordica charantia OX=3673 GN=LOC111012104 PE=3 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 1.0e-237
Identity = 415/536 (77.43%), Postives = 431/536 (80.41%), Query Frame = 0

Query: 1   MISEAVENGGSDMVIDGIEQKPKKIVPDRFDANYLKIYYGKLFPYTDFFKWMSYGNDGNH 60
           MI+EAVENGG DM IDG EQKP+K+VPD FD NYLKIYYGKLFPYTDFFKWMSYGNDG H
Sbjct: 1   MITEAVENGGGDMEIDGFEQKPEKVVPDGFDVNYLKIYYGKLFPYTDFFKWMSYGNDGKH 60

Query: 61  PGTDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PG+DQSYFGRREFSFTL+NDIYLR+QSFNNLSEMENSIKEKCPFKIDIGPVYSVD AKRH
Sbjct: 61  PGSDQSYFGRREFSFTLENDIYLRYQSFNNLSEMENSIKEKCPFKIDIGPVYSVDLAKRH 120

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMSAAIKVIDTTLRDDFG 180
           AYAGDNVFTPVEREL+FDIDMTDYDDVRYCCSGADVCLDCWPLM+AAIKVIDTTLRDDFG
Sbjct: 121 AYAGDNVFTPVERELIFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180

Query: 181 FNHLLWVFSGRRGVHCWVCDGKARRLSNEQRAAITEYFHLYQANENSNKKISLTGPVLHP 240
           FNH+LWVFSGRRGVHCWVCDGKARRLSNEQRAAI E+FHLYQ NENS KKISLTGPVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEHFHLYQGNENSRKKISLTGPVLHP 240

Query: 241 FLVRSYTEVLKDSFESKLLSSQKIFSTEERYEKILDMIPDESITSELRGRWQENRRPSKK 300
           FL RSYTEVLKDSFES LL SQKIFSTEERYEKILDMIPDES+TSELRGRWQENR+PSKK
Sbjct: 241 FLARSYTEVLKDSFESTLLCSQKIFSTEERYEKILDMIPDESVTSELRGRWQENRQPSKK 300

Query: 301 DVNSVRWEQLKNVLQSGKHKAHGIRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVN VRWEQLK++LQS KHKA G+RRHVEEIVFSFTYPRLDLEVTKH NHLLKAPFCVHP
Sbjct: 301 DVNYVRWEQLKSMLQSVKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHTNHLLKAPFCVHP 360

Query: 361 KTGRICVPINPERCDEFDPTTVPTIAQLLEELNAADM-------------KVDGETDWDR 420
           KTGRICVPINPE CDEFDPTTVPT+AQLLEELNAADM             KVDGETDWDR
Sbjct: 361 KTGRICVPINPENCDEFDPTTVPTLAQLLEELNAADMNVDGEYSQEKKRQKVDGETDWDR 420

Query: 421 TSLGESIRFFRSSFLQPLLKSCKIFSILAIFYKRFILLLSGKDFCRQNVTKESQAKTIGH 480
           TSLGESIRFFRSSFLQPLLKSCK                                     
Sbjct: 421 TSLGESIRFFRSSFLQPLLKSCK------------------------------------- 463

Query: 481 SFYPLVLRLKHTLNARMLYIFREIEQVLSHDSCLLQEEIESSYNAKVQQSKNSLSW 524
                                               EEIESSYNAKVQQSKN LSW
Sbjct: 481 ------------------------------------EEIESSYNAKVQQSKNPLSW 463

BLAST of Sgr020240 vs. ExPASy TrEMBL
Match: A0A0A0KUP7 (DNA primase OS=Cucumis sativus OX=3659 GN=Csa_5G647410 PE=3 SV=1)

HSP 1 Score: 831.2 bits (2146), Expect = 2.3e-237
Identity = 412/523 (78.78%), Postives = 428/523 (81.84%), Query Frame = 0

Query: 1   MISEAVENGGSDMVIDGIEQKPKKIVPDRFDANYLKIYYGKLFPYTDFFKWMSYGNDGNH 60
           M++ AVENGGSDMVIDG EQKPK  V D FDANYLKIYYGKLFPY DFFKWMSYGNDG H
Sbjct: 1   MVTAAVENGGSDMVIDGNEQKPKNTVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 60

Query: 61  PGTDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PG+DQSYFGRREFSFTLDNDIYLRFQSFNNLSEME SIKEKCPFKIDIGPVYSVDPAKRH
Sbjct: 61  PGSDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMEISIKEKCPFKIDIGPVYSVDPAKRH 120

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMSAAIKVIDTTLRDDFG 180
           AYA DNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLM+AAIKVI TTLRDDFG
Sbjct: 121 AYADDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIHTTLRDDFG 180

Query: 181 FNHLLWVFSGRRGVHCWVCDGKARRLSNEQRAAITEYFHLYQANENSNKKISLTGPVLHP 240
           FNH+LWVFSGRRGVHCWVCDGKARRLSNEQRAAI EYFHLYQ NENS KKISLTGPVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 240

Query: 241 FLVRSYTEVLKDSFESKLLSSQKIFSTEERYEKILDMIPDESITSELRGRWQENRRPSKK 300
           FLVRSYTEVLKDSFES+LL SQKIFSTEERYEKILDMIPDESITS+LRGRWQ+NRR SK 
Sbjct: 241 FLVRSYTEVLKDSFESQLLCSQKIFSTEERYEKILDMIPDESITSDLRGRWQDNRRSSKN 300

Query: 301 DVNSVRWEQLKNVLQSGKHKAHGIRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVNS+RWEQLK++LQSGKHKA G+RRHVEEIVFSFTYPRLD EVTKHMNHLLKAPFCVHP
Sbjct: 301 DVNSLRWEQLKSLLQSGKHKAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHP 360

Query: 361 KTGRICVPINPERCDEFDPTTVPTIAQLLEELNAADMKVDGETDWDRTSLGESIRFFRSS 420
           KTGRICVPI+PE CDEFDPTTVPT+AQLLEELNAADM+VD E DWDRTSLGESIRFFRS+
Sbjct: 361 KTGRICVPIDPEHCDEFDPTTVPTVAQLLEELNAADMEVDSENDWDRTSLGESIRFFRSA 420

Query: 421 FLQPLLKSCKIFSILAIFYKRFILLLSGKDFCRQNVTKESQAKTIGHSFYPLVLRLKHTL 480
           FLQPLLKSCK                                                  
Sbjct: 421 FLQPLLKSCK-------------------------------------------------- 450

Query: 481 NARMLYIFREIEQVLSHDSCLLQEEIESSYNAKVQQSKNSLSW 524
                                  EEIESSYNAKVQQSKNSLSW
Sbjct: 481 -----------------------EEIESSYNAKVQQSKNSLSW 450

BLAST of Sgr020240 vs. ExPASy TrEMBL
Match: A0A6J1EEZ9 (DNA primase OS=Cucurbita moschata OX=3662 GN=LOC111432676 PE=3 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 2.8e-235
Identity = 408/523 (78.01%), Postives = 426/523 (81.45%), Query Frame = 0

Query: 1   MISEAVENGGSDMVIDGIEQKPKKIVPDRFDANYLKIYYGKLFPYTDFFKWMSYGNDGNH 60
           M+ EA ENGGSDMVIDG EQKPK IV + FDANYL+IYYGKLFP+ DFFKWMSYGNDG H
Sbjct: 1   MVHEAAENGGSDMVIDGNEQKPKNIVSNGFDANYLEIYYGKLFPFADFFKWMSYGNDGKH 60

Query: 61  PGTDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PG+D SY GRREFSFTLDNDIYLRFQSFN+LSEME+S KEKCPFKIDIGPVYSVDPAKRH
Sbjct: 61  PGSDHSYIGRREFSFTLDNDIYLRFQSFNSLSEMESSTKEKCPFKIDIGPVYSVDPAKRH 120

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMSAAIKVIDTTLRDDFG 180
           AYAG NVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLM+AAIKVIDTTLRDDFG
Sbjct: 121 AYAGANVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180

Query: 181 FNHLLWVFSGRRGVHCWVCDGKARRLSNEQRAAITEYFHLYQANENSNKKISLTGPVLHP 240
           FNH+LWVFSGRRGVHCWVCDGKARRLSNEQRAAI +YFHLYQ NENS KKISLTGPVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIADYFHLYQGNENSRKKISLTGPVLHP 240

Query: 241 FLVRSYTEVLKDSFESKLLSSQKIFSTEERYEKILDMIPDESITSELRGRWQENRRPSKK 300
           FL RSY EVLKDSFESKLL SQKIFSTEERYEKILDMI DESITSELRGRWQ+NRRPSK 
Sbjct: 241 FLARSYIEVLKDSFESKLLCSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKN 300

Query: 301 DVNSVRWEQLKNVLQSGKHKAHGIRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVN VRWEQLKN+LQSGKHK+ G+RRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP
Sbjct: 301 DVNLVRWEQLKNMLQSGKHKSPGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360

Query: 361 KTGRICVPINPERCDEFDPTTVPTIAQLLEELNAADMKVDGETDWDRTSLGESIRFFRSS 420
           KTGRICVPI+PERCDEFDPTTVPT+AQLLEELNAADM+VD ETDWDRTSLGESIRFFRS+
Sbjct: 361 KTGRICVPIDPERCDEFDPTTVPTVAQLLEELNAADMEVDSETDWDRTSLGESIRFFRSA 420

Query: 421 FLQPLLKSCKIFSILAIFYKRFILLLSGKDFCRQNVTKESQAKTIGHSFYPLVLRLKHTL 480
           FLQPLLKSCK                                                  
Sbjct: 421 FLQPLLKSCK-------------------------------------------------- 450

Query: 481 NARMLYIFREIEQVLSHDSCLLQEEIESSYNAKVQQSKNSLSW 524
                                  EEIESSYNAKVQQSKN LSW
Sbjct: 481 -----------------------EEIESSYNAKVQQSKNPLSW 450

BLAST of Sgr020240 vs. ExPASy TrEMBL
Match: A0A6J1I8T4 (DNA primase OS=Cucurbita maxima OX=3661 GN=LOC111472037 PE=3 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 5.5e-231
Identity = 401/513 (78.17%), Postives = 418/513 (81.48%), Query Frame = 0

Query: 11  SDMVIDGIEQKPKKIVPDRFDANYLKIYYGKLFPYTDFFKWMSYGNDGNHPGTDQSYFGR 70
           +DMVIDG EQKPK IV + FDANYLKIYYGKLFP+ DFFKWMSYGNDG HPG+D SY GR
Sbjct: 6   ADMVIDGNEQKPKNIVSNGFDANYLKIYYGKLFPFADFFKWMSYGNDGKHPGSDHSYVGR 65

Query: 71  REFSFTLDNDIYLRFQSFNNLSEMENSIKEKCPFKIDIGPVYSVDPAKRHAYAGDNVFTP 130
           REFSFTLDNDIYLRFQSFN+LSEME+S KEKCPFKIDIGPVYSVDPAKRHAYAG NVFTP
Sbjct: 66  REFSFTLDNDIYLRFQSFNSLSEMESSTKEKCPFKIDIGPVYSVDPAKRHAYAGANVFTP 125

Query: 131 VERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMSAAIKVIDTTLRDDFGFNHLLWVFSG 190
           VERELVFDIDMTDYDDVRYCCSGADVCLDCWPLM+AAIKVIDTTLRDDFGFNH+LWVFSG
Sbjct: 126 VERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSG 185

Query: 191 RRGVHCWVCDGKARRLSNEQRAAITEYFHLYQANENSNKKISLTGPVLHPFLVRSYTEVL 250
           RRGVHCWVCDGKARRLSNEQRAAI +YFHLYQ NENS KKISLTGPVLHPFL RSY EVL
Sbjct: 186 RRGVHCWVCDGKARRLSNEQRAAIADYFHLYQGNENSRKKISLTGPVLHPFLARSYIEVL 245

Query: 251 KDSFESKLLSSQKIFSTEERYEKILDMIPDESITSELRGRWQENRRPSKKDVNSVRWEQL 310
           KDSFESKLL SQKIFSTEERYEKILDMI DESITSELRGRWQ+NRRPSK DVN VRWEQL
Sbjct: 246 KDSFESKLLCSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKNDVNLVRWEQL 305

Query: 311 KNVLQSGKHKAHGIRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPIN 370
           KN+LQSGKHK+ G+RRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPI+
Sbjct: 306 KNMLQSGKHKSQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPID 365

Query: 371 PERCDEFDPTTVPTIAQLLEELNAADMKVDGETDWDRTSLGESIRFFRSSFLQPLLKSCK 430
           PERCDEFDPTTVPT+AQLLEELNAADM+VD ETDWDRTSLGESIRFFRS+FLQPLLKSCK
Sbjct: 366 PERCDEFDPTTVPTVAQLLEELNAADMEVDSETDWDRTSLGESIRFFRSAFLQPLLKSCK 425

Query: 431 IFSILAIFYKRFILLLSGKDFCRQNVTKESQAKTIGHSFYPLVLRLKHTLNARMLYIFRE 490
                                                                       
Sbjct: 426 ------------------------------------------------------------ 445

Query: 491 IEQVLSHDSCLLQEEIESSYNAKVQQSKNSLSW 524
                        EEIESSYNAKVQQSKN LSW
Sbjct: 486 -------------EEIESSYNAKVQQSKNPLSW 445

BLAST of Sgr020240 vs. ExPASy TrEMBL
Match: A0A5A7T8Y3 (DNA primase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001310 PE=3 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 6.1e-222
Identity = 369/403 (91.56%), Postives = 384/403 (95.29%), Query Frame = 0

Query: 1   MISEAVENGGSDMVIDGIEQKPKKIVPDRFDANYLKIYYGKLFPYTDFFKWMSYGNDGNH 60
           M++ AVENGGSDMVIDG EQKPKK V D FDANYLKIYYGKLFPY DFFKWMSYGNDG H
Sbjct: 16  MVTAAVENGGSDMVIDGNEQKPKKSVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 75

Query: 61  PGTDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PG+DQSYFGRREFSFTLDNDIYLRFQSFNNLSEME SIKE+CPFKIDIGPVYSVDPAKRH
Sbjct: 76  PGSDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMEISIKEQCPFKIDIGPVYSVDPAKRH 135

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMSAAIKVIDTTLRDDFG 180
           AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLM+AAIKVIDTTLRDDFG
Sbjct: 136 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 195

Query: 181 FNHLLWVFSGRRGVHCWVCDGKARRLSNEQRAAITEYFHLYQANENSNKKISLTGPVLHP 240
           FNH+LWVFSGRRGVHCWVCDGKARRLSNEQRAAI EYFHLYQ NENS KKISLTGPVLHP
Sbjct: 196 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 255

Query: 241 FLVRSYTEVLKDSFESKLLSSQKIFSTEERYEKILDMIPDESITSELRGRWQENRRPSKK 300
           FL RSYTEVLKDSFES+LL  QKIFSTEERYEKILDMIPDESITS+LRGRWQ+NRR SK 
Sbjct: 256 FLARSYTEVLKDSFESQLLCRQKIFSTEERYEKILDMIPDESITSDLRGRWQDNRRSSKN 315

Query: 301 DVNSVRWEQLKNVLQSGKHKAHGIRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVNS+RWEQLKN+LQSGKHKA G+RRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP
Sbjct: 316 DVNSLRWEQLKNLLQSGKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 375

Query: 361 KTGRICVPINPERCDEFDPTTVPTIAQLLEELNAADMKVDGET 404
           KTGRICVPI+PE CDEFDPTTVPT+AQLLEELNAADM+VD ET
Sbjct: 376 KTGRICVPIDPEHCDEFDPTTVPTVAQLLEELNAADMEVDSET 418

BLAST of Sgr020240 vs. TAIR 10
Match: AT5G41880.1 (DNA primases;DNA primases )

HSP 1 Score: 597.8 bits (1540), Expect = 8.3e-171
Identity = 281/435 (64.60%), Postives = 347/435 (79.77%), Query Frame = 0

Query: 1   MISEAVENGGSDMVIDGIEQKPKKIVPDRFDANYLKIYYGKLFPYTDFFKWMSYGNDGNH 60
           M  E  E  G DM+ID     PK  + + F+ +YL+IYYG LFPYTD  KW+SYG+DG H
Sbjct: 1   MTREETETSGEDMMID----DPKPKIENDFNVHYLRIYYGNLFPYTDIHKWLSYGHDGKH 60

Query: 61  PGTDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PG D+ YFGRREFSFTL+ND+YLR++SF N S ME +IK   P+KIDIG VYSVDP KRH
Sbjct: 61  PGCDEYYFGRREFSFTLENDVYLRYKSFKNASLMEAAIKSNFPYKIDIGAVYSVDPDKRH 120

Query: 121 AYA--GDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMSAAIKVIDTTLRDD 180
           AYA  G N+FTPVERELVFDID+TDYDDVRYCCSGADVC  CWPLM+ AIKVIDT+LR+D
Sbjct: 121 AYAQSGTNLFTPVERELVFDIDITDYDDVRYCCSGADVCSKCWPLMTVAIKVIDTSLRED 180

Query: 181 FGFNHLLWVFSGRRGVHCWVCDGKARRLSNEQRAAITEYFHLYQANENSNKKISLTGPVL 240
           FGF H+LWVFSGRRGVHCWVCD KARRL+NEQR+A+ EYF +Y+ NEN+ KK+ L G  L
Sbjct: 181 FGFKHILWVFSGRRGVHCWVCDAKARRLTNEQRSAVAEYFRVYKGNENNAKKVDLMGHSL 240

Query: 241 HPFLVRSYTEVLKDSFESKLLSSQKIFSTEERYEKILDMIPDESITSELRGRWQENRRP- 300
           HPFL RSY + +K  FE +L ++Q IFS++E+YEKIL MI DE I ++LRG+W+ + R  
Sbjct: 241 HPFLARSYVDFIKSFFEGELQATQSIFSSKEKYEKILGMITDEDIQADLRGKWENSARSS 300

Query: 301 -SKKDVNSVRWEQLKNVLQSGKHKAHGIRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPF 360
            S++  + +RWEQLK  LQS ++KA  +R  VEEIVF+FTYPR+DLEV+K MNHLLKAPF
Sbjct: 301 LSEEATSLLRWEQLKKALQSKRNKALSLRTCVEEIVFTFTYPRIDLEVSKQMNHLLKAPF 360

Query: 361 CVHPKTGRICVPINPERCDEFDPTTVPTIAQLLEELNAADMKVD-GETDWDRTSLGESIR 420
           CVHPKTGR+CVPI+P  CDEFDP  VPT++QL+EE+N+  +++D  + D D + LG+SI+
Sbjct: 361 CVHPKTGRVCVPIDPNNCDEFDPLAVPTLSQLIEEINSGGLRMDVDDDDSDSSLLGKSIK 420

Query: 421 FFRSSFLQPLLKSCK 431
           FFRSSFL+PLLKSCK
Sbjct: 421 FFRSSFLEPLLKSCK 431

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141835.12.1e-23777.43DNA primase small subunit-like [Momordica charantia] >XP_022141836.1 DNA primase... [more]
XP_004141619.14.8e-23778.78DNA primase small subunit [Cucumis sativus] >XP_031742330.1 DNA primase small su... [more]
XP_023535939.11.5e-23578.20DNA primase small subunit [Cucurbita pepo subsp. pepo][more]
KAG6592333.14.5e-23577.82DNA primase small subunit, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022925368.15.9e-23578.01DNA primase small subunit [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
P496423.4e-9245.28DNA primase small subunit OS=Homo sapiens OX=9606 GN=PRIM1 PE=1 SV=1[more]
P206645.7e-9245.48DNA primase small subunit OS=Mus musculus OX=10090 GN=Prim1 PE=1 SV=1[more]
Q243176.8e-8541.01DNA primase small subunit OS=Drosophila melanogaster OX=7227 GN=Prim1 PE=1 SV=2[more]
O142151.4e-8238.85DNA primase small subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843)... [more]
Q7KQM16.0e-7336.32DNA primase small subunit OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=PF1... [more]
Match NameE-valueIdentityDescription
A0A6J1CKZ31.0e-23777.43DNA primase OS=Momordica charantia OX=3673 GN=LOC111012104 PE=3 SV=1[more]
A0A0A0KUP72.3e-23778.78DNA primase OS=Cucumis sativus OX=3659 GN=Csa_5G647410 PE=3 SV=1[more]
A0A6J1EEZ92.8e-23578.01DNA primase OS=Cucurbita moschata OX=3662 GN=LOC111432676 PE=3 SV=1[more]
A0A6J1I8T45.5e-23178.17DNA primase OS=Cucurbita maxima OX=3661 GN=LOC111472037 PE=3 SV=1[more]
A0A5A7T8Y36.1e-22291.56DNA primase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001310 P... [more]
Match NameE-valueIdentityDescription
AT5G41880.18.3e-17164.60DNA primases;DNA primases [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.920.10DNA primase, PRIM domaincoord: 23..431
e-value: 1.8E-152
score: 509.9
NoneNo IPR availablePANTHERPTHR10536:SF1DNA PRIMASEcoord: 41..430
NoneNo IPR availableSUPERFAMILY56747Prim-pol domaincoord: 31..397
IPR014052DNA primase, small subunit, eukaryotic/archaealTIGRFAMTIGR00335TIGR00335coord: 36..388
e-value: 1.6E-64
score: 216.3
IPR014052DNA primase, small subunit, eukaryotic/archaealPANTHERPTHR10536DNA PRIMASE SMALL SUBUNITcoord: 41..430
IPR014052DNA primase, small subunit, eukaryotic/archaealCDDcd04860AE_Prim_Scoord: 44..379
e-value: 3.74599E-92
score: 279.113
IPR002755DNA primase, small subunitPFAMPF01896DNA_primase_Scoord: 137..370
e-value: 7.7E-62
score: 208.5

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr020240.1Sgr020240.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006269 DNA replication, synthesis of RNA primer
cellular_component GO:0005658 alpha DNA polymerase:primase complex
molecular_function GO:0003896 DNA primase activity
molecular_function GO:0046872 metal ion binding