Sgr020054 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr020054 vs. NCBI nr
Match: KAG7031391.1 (WD repeat-containing protein 25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 560.5 bits (1443), Expect = 1.1e-155
Identity = 281/325 (86.46%), Postives = 309/325 (95.08%), Query Frame = 0

Query: 35  RCSSLNFGAMESLFEAYSNASDDDDEEEEEPKPKRLALPSSSSKRPKPERSKFLPKFQSS 94
           +CSS NFGAME LFEAYSN SD+D+ +E+EP+PKRLALPSS+SKR  PE S+F P F+ S
Sbjct: 33  QCSSHNFGAMEHLFEAYSNTSDEDN-QEDEPQPKRLALPSSTSKR--PEHSQFQPMFRPS 92

Query: 95  HCPDLNSEPQKGIMVPGRYVSKRERALLSSAPVSRAPDSFLNPSVPSSTVGSLSDSDLPC 154
           +CPD  SEPQK IMVPGRY+SKRERAL+SSAPVSRAP+SFLNPSV SSTVGSLSDSDLP 
Sbjct: 93  NCPDSTSEPQKEIMVPGRYISKRERALMSSAPVSRAPESFLNPSVQSSTVGSLSDSDLPR 152

Query: 155 DIVSLLRSRAKDYLQQCLMPERMSVTLDCHTKAVNAVQWSPSHAHLLASAAMDHTICIWN 214
           DI+SLLRSRAK+YLQQ LMP+RMSV+LDCHTKAVNAVQWSPSHAHLLASAAMD+TICIWN
Sbjct: 153 DILSLLRSRAKNYLQQGLMPKRMSVSLDCHTKAVNAVQWSPSHAHLLASAAMDYTICIWN 212

Query: 215 VWSTEQKLAFKSSIHDAAVKNVQWSQQGLSILSCGYDCASRLIDVEKGTELGVFKEDQGV 274
           +WSTEQKLAFKSSIHDAAVK+V+WSQQGLSILSCGYDC+SRLIDVE+GTE+GVFKEDQGV
Sbjct: 213 IWSTEQKLAFKSSIHDAAVKDVEWSQQGLSILSCGYDCSSRLIDVEEGTEVGVFKEDQGV 272

Query: 275 AVVKFHPNNPNLFLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTPNGNQFISSSD 334
           AVVKFHPNNP+LFLSGGLKGSLRMWD+RIGKVVNKYNRRLGPILDVEFTPNGNQFISSSD
Sbjct: 273 AVVKFHPNNPSLFLSGGLKGSLRMWDVRIGKVVNKYNRRLGPILDVEFTPNGNQFISSSD 332

Query: 335 VSASNSSESSIIVWDVTREVPLSYQ 360
           VS+SNSSESS+IVWDVTREVPLSYQ
Sbjct: 333 VSSSNSSESSVIVWDVTREVPLSYQ 354

BLAST of Sgr020054 vs. NCBI nr
Match: XP_022142879.1 (WD repeat-containing protein 25 [Momordica charantia])

HSP 1 Score: 552.4 bits (1422), Expect = 2.9e-153
Identity = 280/316 (88.61%), Postives = 297/316 (93.99%), Query Frame = 0

Query: 44  MESLFEAYSNASDDDDEEEEEPKPKRLALPSSSSKRPKPERSKFLPKFQSSHCPDLNSEP 103
           MESLFEAYSNAS+DD   E+EP+PKRLALPSS SKRPKPERSKF PKFQ S  PD NSEP
Sbjct: 1   MESLFEAYSNASEDD---EDEPQPKRLALPSSPSKRPKPERSKFPPKFQPSPSPDFNSEP 60

Query: 104 QKGIMVPGRYVSKRERALLSSAPVSRAPDSFLNPSVPSSTVGSLSDSDLPCDIVSLLRSR 163
           QKGIMVPGRYVSKRERALL +APVS APDSFLNPSV SSTVG+LSDSDLP DI+SLLRSR
Sbjct: 61  QKGIMVPGRYVSKRERALLGAAPVSTAPDSFLNPSVLSSTVGNLSDSDLPRDILSLLRSR 120

Query: 164 AKDYLQQCLMPERMSVTLDCHTKAVNAVQWSPSHAHLLASAAMDHTICIWNVWSTEQKLA 223
           AKD LQQ +MPERMSVTLDCHTKAVNA+QWSPS+AHLLASAAMDHTIC+WNVW+TEQKLA
Sbjct: 121 AKDCLQQGMMPERMSVTLDCHTKAVNALQWSPSNAHLLASAAMDHTICVWNVWNTEQKLA 180

Query: 224 FKSSIHDAAVKNVQWSQQGLSILSCGYDCASRLIDVEKGTELGVFKEDQGVAVVKFHPNN 283
           FKS+IH+AAVKNVQWSQ+GLSILSCGYD ASRLIDVEKGTE+G FKEDQGVAVVKFHPNN
Sbjct: 181 FKSNIHNAAVKNVQWSQEGLSILSCGYDRASRLIDVEKGTEVGTFKEDQGVAVVKFHPNN 240

Query: 284 PNLFLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVSASNSSES 343
           PNLFLSGGLKGSLRMWD RIGKVVNKYNRRLGPILDVEFTP+ NQFISSSDVSASNSSES
Sbjct: 241 PNLFLSGGLKGSLRMWDTRIGKVVNKYNRRLGPILDVEFTPHSNQFISSSDVSASNSSES 300

Query: 344 SIIVWDVTREVPLSYQ 360
           SIIVWD+TREVPLSYQ
Sbjct: 301 SIIVWDITREVPLSYQ 313

BLAST of Sgr020054 vs. NCBI nr
Match: XP_022941667.1 (WD repeat-containing protein 25 [Cucurbita moschata])

HSP 1 Score: 542.7 bits (1397), Expect = 2.3e-150
Identity = 272/316 (86.08%), Postives = 300/316 (94.94%), Query Frame = 0

Query: 44  MESLFEAYSNASDDDDEEEEEPKPKRLALPSSSSKRPKPERSKFLPKFQSSHCPDLNSEP 103
           ME LFEAYSN SD+D+ +E+EP+PKRLALPSS+SKR  PE S+F P F+ SH PD  SEP
Sbjct: 1   MEHLFEAYSNTSDEDN-QEDEPQPKRLALPSSTSKR--PEHSQFQPMFRPSHSPDSTSEP 60

Query: 104 QKGIMVPGRYVSKRERALLSSAPVSRAPDSFLNPSVPSSTVGSLSDSDLPCDIVSLLRSR 163
           QK IMVPGRY+SKRERAL+SSAPVSRAP+SFLNPSV SSTVGSLSDSDLP DI+SLLRSR
Sbjct: 61  QKEIMVPGRYISKRERALMSSAPVSRAPESFLNPSVQSSTVGSLSDSDLPRDILSLLRSR 120

Query: 164 AKDYLQQCLMPERMSVTLDCHTKAVNAVQWSPSHAHLLASAAMDHTICIWNVWSTEQKLA 223
           AK+YLQQ LMP+RMSV+LDCHTKAVNAVQWSPSHAHLLASAAMD+TICIWN+WSTEQKLA
Sbjct: 121 AKNYLQQGLMPKRMSVSLDCHTKAVNAVQWSPSHAHLLASAAMDYTICIWNIWSTEQKLA 180

Query: 224 FKSSIHDAAVKNVQWSQQGLSILSCGYDCASRLIDVEKGTELGVFKEDQGVAVVKFHPNN 283
           FKSSIH+AAVK+V+WSQQGLSILSCGYDC+SRLIDVEKGTE+G+FKEDQGVAVVKFHPNN
Sbjct: 181 FKSSIHNAAVKDVEWSQQGLSILSCGYDCSSRLIDVEKGTEVGLFKEDQGVAVVKFHPNN 240

Query: 284 PNLFLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVSASNSSES 343
           P++FLSGGLKGSLRMWD+RIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVS+SNSSES
Sbjct: 241 PSVFLSGGLKGSLRMWDVRIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVSSSNSSES 300

Query: 344 SIIVWDVTREVPLSYQ 360
           S+IVWDVTREVPLSYQ
Sbjct: 301 SVIVWDVTREVPLSYQ 313

BLAST of Sgr020054 vs. NCBI nr
Match: KAG6600751.1 (WD repeat-containing protein 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 542.3 bits (1396), Expect = 3.0e-150
Identity = 272/316 (86.08%), Postives = 299/316 (94.62%), Query Frame = 0

Query: 44  MESLFEAYSNASDDDDEEEEEPKPKRLALPSSSSKRPKPERSKFLPKFQSSHCPDLNSEP 103
           ME LFEAYSN SD+D+ +E+EP+PKRLALPSS+SKR  PE S+F P F+ S+CPD  SEP
Sbjct: 1   MEHLFEAYSNTSDEDN-QEDEPQPKRLALPSSTSKR--PEHSQFQPMFRPSNCPDSTSEP 60

Query: 104 QKGIMVPGRYVSKRERALLSSAPVSRAPDSFLNPSVPSSTVGSLSDSDLPCDIVSLLRSR 163
           QK IMVPGRY+SKRERAL+SSAPVSRAP+SFLNPSV SSTVGSLSDSDLP DI+SLLRSR
Sbjct: 61  QKEIMVPGRYISKRERALMSSAPVSRAPESFLNPSVQSSTVGSLSDSDLPRDILSLLRSR 120

Query: 164 AKDYLQQCLMPERMSVTLDCHTKAVNAVQWSPSHAHLLASAAMDHTICIWNVWSTEQKLA 223
           AK+YLQQ LMP+RMSV+LDCHTKAVNAVQWSPSHAHLLAS AMD+TICIWN+WSTEQKLA
Sbjct: 121 AKNYLQQGLMPKRMSVSLDCHTKAVNAVQWSPSHAHLLASVAMDYTICIWNIWSTEQKLA 180

Query: 224 FKSSIHDAAVKNVQWSQQGLSILSCGYDCASRLIDVEKGTELGVFKEDQGVAVVKFHPNN 283
           FKSSIH+AAVK+V+WSQQGLSILSCGYDC+SRLIDVE GTE+GVFKEDQGVAVVKFHPNN
Sbjct: 181 FKSSIHNAAVKDVEWSQQGLSILSCGYDCSSRLIDVEDGTEVGVFKEDQGVAVVKFHPNN 240

Query: 284 PNLFLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVSASNSSES 343
           P+LFLSGGLKGSLRMWD+RIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVS+SNSSES
Sbjct: 241 PSLFLSGGLKGSLRMWDVRIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVSSSNSSES 300

Query: 344 SIIVWDVTREVPLSYQ 360
           S+IVWDVTREVPLSYQ
Sbjct: 301 SVIVWDVTREVPLSYQ 313

BLAST of Sgr020054 vs. NCBI nr
Match: XP_022992622.1 (WD repeat-containing protein 25 [Cucurbita maxima])

HSP 1 Score: 541.6 bits (1394), Expect = 5.2e-150
Identity = 272/316 (86.08%), Postives = 299/316 (94.62%), Query Frame = 0

Query: 44  MESLFEAYSNASDDDDEEEEEPKPKRLALPSSSSKRPKPERSKFLPKFQSSHCPDLNSEP 103
           ME LFEAYSN SD+D+ EE+EP+PKRLALPSS+SKR  PE S+F P F+ SHCPD  SEP
Sbjct: 1   MEHLFEAYSNTSDEDN-EEDEPQPKRLALPSSTSKR--PEHSQFQPMFRPSHCPDSTSEP 60

Query: 104 QKGIMVPGRYVSKRERALLSSAPVSRAPDSFLNPSVPSSTVGSLSDSDLPCDIVSLLRSR 163
           QK IMVPGRY+SKRERAL+SSAPVSRAP+SFLNPSV  STVGSLSDSDLP DI+SLLRSR
Sbjct: 61  QKEIMVPGRYISKRERALMSSAPVSRAPESFLNPSVQFSTVGSLSDSDLPRDILSLLRSR 120

Query: 164 AKDYLQQCLMPERMSVTLDCHTKAVNAVQWSPSHAHLLASAAMDHTICIWNVWSTEQKLA 223
           AK+YLQQ LMP+RMSV+LDCHTKAVNAVQWSPSHAHLLASAAMD+TICIWN+WSTEQKLA
Sbjct: 121 AKNYLQQGLMPKRMSVSLDCHTKAVNAVQWSPSHAHLLASAAMDYTICIWNIWSTEQKLA 180

Query: 224 FKSSIHDAAVKNVQWSQQGLSILSCGYDCASRLIDVEKGTELGVFKEDQGVAVVKFHPNN 283
           FKSSIH+AAVK+V+WSQQGLSILSCGYDC+SRLIDVEKGTE+GVFKEDQGVAVVKFHPN+
Sbjct: 181 FKSSIHNAAVKDVEWSQQGLSILSCGYDCSSRLIDVEKGTEIGVFKEDQGVAVVKFHPND 240

Query: 284 PNLFLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVSASNSSES 343
           P+LFLSGG+KGSLRMWD+RIGKVVNKYNRRLGPILDVEFTPN NQFISSSDVS+SNSSES
Sbjct: 241 PSLFLSGGVKGSLRMWDVRIGKVVNKYNRRLGPILDVEFTPNVNQFISSSDVSSSNSSES 300

Query: 344 SIIVWDVTREVPLSYQ 360
           S+IVWDVTREVPLSYQ
Sbjct: 301 SVIVWDVTREVPLSYQ 313

BLAST of Sgr020054 vs. ExPASy Swiss-Prot
Match: E9Q349 (WD repeat-containing protein 25 OS=Mus musculus OX=10090 GN=Wdr25 PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.0e-31
Identity = 87/275 (31.64%), Postives = 138/275 (50.18%), Query Frame = 0

Query: 91  FQSSHCPDLNSEPQKGIMVPGRYVSKRERALLSSAPVSRAPDSFLNPSVPSSTVGSLSDS 150
           F+S+     +S+ ++G      Y+ KR R L +  P +        P  P+        +
Sbjct: 145 FESTAGNASSSQRKRGEDCVLPYIPKRLRQLQALNPEAGGGKDGEPPGPPAGC------A 204

Query: 151 DLPCDIVSLLRSRAKDYL----QQCLMPERMSVTLDCHTKAVNAVQWSP--SHAHLLASA 210
             P  +   +    + YL    ++  +P+++   L  H   VN++QW P    +H+L SA
Sbjct: 205 PAPLCVAPTVSEFIQPYLNSQYRETTVPKKVLFHLRGHRGPVNSIQWCPVFCKSHMLLSA 264

Query: 211 AMDHTICIWNVWSTEQKLAFKSSIHDAAVKNVQWSQQGLSILSCGYDCASRLIDVEKGTE 270
           +MD T  +WN   +   L    S+H  AV+  +WS  G  ILS G+D A  L D+E GT+
Sbjct: 265 SMDKTFKVWNAVDSGHCLQ-TYSVHSEAVRAARWSPCGRRILSGGFDFALHLTDLETGTQ 324

Query: 271 LGVFKEDQGVAVVKFHPNNPNLFLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTP 330
           +   + D  V  +KFHP + N+FL GG    ++ WDMR GKVV  Y   +   LD+ F  
Sbjct: 325 VFSGQSDFRVTTLKFHPKDHNVFLCGGFSSEIKAWDMRTGKVVKGYKATIQQTLDILFLQ 384

Query: 331 NGNQFISSSDVSASNSSESSIIVWDVTREVPLSYQ 360
            G++F+SS+D S  +S++ +II WD      +S Q
Sbjct: 385 EGSEFLSSTDASTRDSADRTIIAWDFRTAAKISNQ 412

BLAST of Sgr020054 vs. ExPASy Swiss-Prot
Match: Q64LD2 (WD repeat-containing protein 25 OS=Homo sapiens OX=9606 GN=WDR25 PE=1 SV=3)

HSP 1 Score: 137.5 bits (345), Expect = 3.0e-31
Identity = 69/195 (35.38%), Postives = 108/195 (55.38%), Query Frame = 0

Query: 167 YLQQCLMPERMSVTLDCHTKAVNAVQWSP--SHAHLLASAAMDHTICIWNVWSTEQKLAF 226
           + ++  +P ++   L  H   VN +QW P  S +H+L S +MD T  +WN   +   L  
Sbjct: 228 HYKETTVPRKVLFHLRGHRGPVNTIQWCPVLSKSHMLLSTSMDKTFKVWNAVDSGHCLQ- 287

Query: 227 KSSIHDAAVKNVQWSQQGLSILSCGYDCASRLIDVEKGTELGVFKEDQGVAVVKFHPNNP 286
             S+H  AV+  +W+  G  ILS G+D A  L D+E GT+L   + D  +  +KFHP + 
Sbjct: 288 TYSLHTEAVRAARWAPCGRRILSGGFDFALHLTDLETGTQLFSGRSDFRITTLKFHPKDH 347

Query: 287 NLFLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVSASNSSESS 346
           N+FL GG    ++ WD+R GKV+  Y   +   LD+ F   G++F+SS+D S  +S++ +
Sbjct: 348 NIFLCGGFSSEMKAWDIRTGKVMRSYKATIQQTLDILFLREGSEFLSSTDASTRDSADRT 407

Query: 347 IIVWDVTREVPLSYQ 360
           II WD      +S Q
Sbjct: 408 IIAWDFRTSAKISNQ 421

BLAST of Sgr020054 vs. ExPASy Swiss-Prot
Match: O43071 (Pre-mRNA-processing factor 17 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=prp17 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 5.6e-22
Identity = 61/192 (31.77%), Postives = 99/192 (51.56%), Query Frame = 0

Query: 169 QQCLMPERMSVTLDCHTKAVNAVQWSPSHAHLLASAAMDHTICIWNVWSTEQKLAFKSSI 228
           Q C +P++   T   HTK ++ +++ P   HLL S +MD+ I IW V+  ++ L      
Sbjct: 250 QTCYIPKKHIFTWKGHTKGISCLRFFPISGHLLLSGSMDNQIKIWEVYH-DRSLLRTFQG 309

Query: 229 HDAAVKNVQWSQQGLSILSCGYDCASRLIDVEKGTELGVFKEDQGVAVVKFH--PNNPNL 288
           H   ++++ +SQ G S LS  +D   +L D E G  L  F  D+    VKF   P+ PN 
Sbjct: 310 HARPIRDLSFSQDGRSFLSTSFDKTIKLWDTELGKCLNCFNSDRLTNCVKFQVDPDKPNE 369

Query: 289 FLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVSASNSSESSII 348
           FL+G     +  +D+R   +V  Y+  LG I  + F  NG +F+++SD       +SS+ 
Sbjct: 370 FLAGTADKRILQFDIRSPDIVQAYDHHLGGINSITFLENGKRFVTTSD-------DSSMR 429

Query: 349 VWDVTREVPLSY 359
            W+    VP+ +
Sbjct: 430 FWEYGTPVPIKF 433

BLAST of Sgr020054 vs. ExPASy Swiss-Prot
Match: O60508 (Pre-mRNA-processing factor 17 OS=Homo sapiens OX=9606 GN=CDC40 PE=1 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 2.4e-20
Identity = 64/209 (30.62%), Postives = 104/209 (49.76%), Query Frame = 0

Query: 152 LPCDIVSLLRSRAKDYLQQCLMPERMSVTLDCHTKAVNAVQWSPSHAHLLASAAMDHTIC 211
           +P D+   LRS      ++C +P++       HTK V+AV+  P   HLL S +MD  I 
Sbjct: 257 IPQDVGVNLRSTMPP--EKCYLPKKQIHVWSGHTKGVSAVRLFPLSGHLLLSCSMDCKIK 316

Query: 212 IWNVWSTEQKLAFKSSIHDAAVKNVQWSQQGLSILSCGYDCASRLIDVEKGTELGVFKED 271
           +W V+  E++       H  AV+++ ++  G   LS  YD   +L D E G  +  F   
Sbjct: 317 LWEVYG-ERRCLRTFIGHSKAVRDICFNTAGTQFLSAAYDRYLKLWDTETGQCISRFTNR 376

Query: 272 QGVAVVKFHP--NNPNLFLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTPNGNQF 331
           +    VKF+P  +  NLF++G     +  WD+R G++V +Y+R LG +  + F     +F
Sbjct: 377 KVPYCVKFNPDEDKQNLFVAGMSDKKIVQWDIRSGEIVQEYDRHLGAVNTIVFVDENRRF 436

Query: 332 ISSSDVSASNSSESSIIVWDVTREVPLSY 359
           +S+SD       + S+ VW+    V   Y
Sbjct: 437 VSTSD-------DKSLRVWEWDIPVDFKY 455

BLAST of Sgr020054 vs. ExPASy Swiss-Prot
Match: Q9DC48 (Pre-mRNA-processing factor 17 OS=Mus musculus OX=10090 GN=Cdc40 PE=2 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 2.4e-20
Identity = 64/209 (30.62%), Postives = 103/209 (49.28%), Query Frame = 0

Query: 152 LPCDIVSLLRSRAKDYLQQCLMPERMSVTLDCHTKAVNAVQWSPSHAHLLASAAMDHTIC 211
           +P D+   LRS      ++C +P++       HTK V+AV+  P   HLL S +MD  I 
Sbjct: 257 IPQDVGVNLRSSVPP--EKCYLPKKQIHVWSGHTKGVSAVRLFPLSGHLLLSCSMDCKIK 316

Query: 212 IWNVWSTEQKLAFKSSIHDAAVKNVQWSQQGLSILSCGYDCASRLIDVEKGTELGVFKED 271
           +W V+   + L      H  AV+++ ++  G   LS  YD   +L D E G  +  F   
Sbjct: 317 LWEVYGDRRCLRTFIG-HSKAVRDICFNTAGTQFLSAAYDRYLKLWDTETGQCISRFTNR 376

Query: 272 QGVAVVKFHP--NNPNLFLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTPNGNQF 331
           +    VKF+P  +  NLF++G     +  WD+R G++V +Y+R LG +  + F     +F
Sbjct: 377 KVPYCVKFNPDEDKQNLFVAGMSDKKIVQWDIRSGEIVQEYDRHLGAVNTIVFVDENRRF 436

Query: 332 ISSSDVSASNSSESSIIVWDVTREVPLSY 359
           +S+SD       + S+ VW+    V   Y
Sbjct: 437 VSTSD-------DKSLRVWEWDIPVDFKY 455

BLAST of Sgr020054 vs. ExPASy TrEMBL
Match: A0A6J1CMQ5 (WD repeat-containing protein 25 OS=Momordica charantia OX=3673 GN=LOC111012887 PE=4 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.4e-153
Identity = 280/316 (88.61%), Postives = 297/316 (93.99%), Query Frame = 0

Query: 44  MESLFEAYSNASDDDDEEEEEPKPKRLALPSSSSKRPKPERSKFLPKFQSSHCPDLNSEP 103
           MESLFEAYSNAS+DD   E+EP+PKRLALPSS SKRPKPERSKF PKFQ S  PD NSEP
Sbjct: 1   MESLFEAYSNASEDD---EDEPQPKRLALPSSPSKRPKPERSKFPPKFQPSPSPDFNSEP 60

Query: 104 QKGIMVPGRYVSKRERALLSSAPVSRAPDSFLNPSVPSSTVGSLSDSDLPCDIVSLLRSR 163
           QKGIMVPGRYVSKRERALL +APVS APDSFLNPSV SSTVG+LSDSDLP DI+SLLRSR
Sbjct: 61  QKGIMVPGRYVSKRERALLGAAPVSTAPDSFLNPSVLSSTVGNLSDSDLPRDILSLLRSR 120

Query: 164 AKDYLQQCLMPERMSVTLDCHTKAVNAVQWSPSHAHLLASAAMDHTICIWNVWSTEQKLA 223
           AKD LQQ +MPERMSVTLDCHTKAVNA+QWSPS+AHLLASAAMDHTIC+WNVW+TEQKLA
Sbjct: 121 AKDCLQQGMMPERMSVTLDCHTKAVNALQWSPSNAHLLASAAMDHTICVWNVWNTEQKLA 180

Query: 224 FKSSIHDAAVKNVQWSQQGLSILSCGYDCASRLIDVEKGTELGVFKEDQGVAVVKFHPNN 283
           FKS+IH+AAVKNVQWSQ+GLSILSCGYD ASRLIDVEKGTE+G FKEDQGVAVVKFHPNN
Sbjct: 181 FKSNIHNAAVKNVQWSQEGLSILSCGYDRASRLIDVEKGTEVGTFKEDQGVAVVKFHPNN 240

Query: 284 PNLFLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVSASNSSES 343
           PNLFLSGGLKGSLRMWD RIGKVVNKYNRRLGPILDVEFTP+ NQFISSSDVSASNSSES
Sbjct: 241 PNLFLSGGLKGSLRMWDTRIGKVVNKYNRRLGPILDVEFTPHSNQFISSSDVSASNSSES 300

Query: 344 SIIVWDVTREVPLSYQ 360
           SIIVWD+TREVPLSYQ
Sbjct: 301 SIIVWDITREVPLSYQ 313

BLAST of Sgr020054 vs. ExPASy TrEMBL
Match: A0A6J1FN47 (WD repeat-containing protein 25 OS=Cucurbita moschata OX=3662 GN=LOC111446958 PE=4 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 1.1e-150
Identity = 272/316 (86.08%), Postives = 300/316 (94.94%), Query Frame = 0

Query: 44  MESLFEAYSNASDDDDEEEEEPKPKRLALPSSSSKRPKPERSKFLPKFQSSHCPDLNSEP 103
           ME LFEAYSN SD+D+ +E+EP+PKRLALPSS+SKR  PE S+F P F+ SH PD  SEP
Sbjct: 1   MEHLFEAYSNTSDEDN-QEDEPQPKRLALPSSTSKR--PEHSQFQPMFRPSHSPDSTSEP 60

Query: 104 QKGIMVPGRYVSKRERALLSSAPVSRAPDSFLNPSVPSSTVGSLSDSDLPCDIVSLLRSR 163
           QK IMVPGRY+SKRERAL+SSAPVSRAP+SFLNPSV SSTVGSLSDSDLP DI+SLLRSR
Sbjct: 61  QKEIMVPGRYISKRERALMSSAPVSRAPESFLNPSVQSSTVGSLSDSDLPRDILSLLRSR 120

Query: 164 AKDYLQQCLMPERMSVTLDCHTKAVNAVQWSPSHAHLLASAAMDHTICIWNVWSTEQKLA 223
           AK+YLQQ LMP+RMSV+LDCHTKAVNAVQWSPSHAHLLASAAMD+TICIWN+WSTEQKLA
Sbjct: 121 AKNYLQQGLMPKRMSVSLDCHTKAVNAVQWSPSHAHLLASAAMDYTICIWNIWSTEQKLA 180

Query: 224 FKSSIHDAAVKNVQWSQQGLSILSCGYDCASRLIDVEKGTELGVFKEDQGVAVVKFHPNN 283
           FKSSIH+AAVK+V+WSQQGLSILSCGYDC+SRLIDVEKGTE+G+FKEDQGVAVVKFHPNN
Sbjct: 181 FKSSIHNAAVKDVEWSQQGLSILSCGYDCSSRLIDVEKGTEVGLFKEDQGVAVVKFHPNN 240

Query: 284 PNLFLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVSASNSSES 343
           P++FLSGGLKGSLRMWD+RIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVS+SNSSES
Sbjct: 241 PSVFLSGGLKGSLRMWDVRIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVSSSNSSES 300

Query: 344 SIIVWDVTREVPLSYQ 360
           S+IVWDVTREVPLSYQ
Sbjct: 301 SVIVWDVTREVPLSYQ 313

BLAST of Sgr020054 vs. ExPASy TrEMBL
Match: A0A6J1JQD9 (WD repeat-containing protein 25 OS=Cucurbita maxima OX=3661 GN=LOC111488910 PE=4 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.5e-150
Identity = 272/316 (86.08%), Postives = 299/316 (94.62%), Query Frame = 0

Query: 44  MESLFEAYSNASDDDDEEEEEPKPKRLALPSSSSKRPKPERSKFLPKFQSSHCPDLNSEP 103
           ME LFEAYSN SD+D+ EE+EP+PKRLALPSS+SKR  PE S+F P F+ SHCPD  SEP
Sbjct: 1   MEHLFEAYSNTSDEDN-EEDEPQPKRLALPSSTSKR--PEHSQFQPMFRPSHCPDSTSEP 60

Query: 104 QKGIMVPGRYVSKRERALLSSAPVSRAPDSFLNPSVPSSTVGSLSDSDLPCDIVSLLRSR 163
           QK IMVPGRY+SKRERAL+SSAPVSRAP+SFLNPSV  STVGSLSDSDLP DI+SLLRSR
Sbjct: 61  QKEIMVPGRYISKRERALMSSAPVSRAPESFLNPSVQFSTVGSLSDSDLPRDILSLLRSR 120

Query: 164 AKDYLQQCLMPERMSVTLDCHTKAVNAVQWSPSHAHLLASAAMDHTICIWNVWSTEQKLA 223
           AK+YLQQ LMP+RMSV+LDCHTKAVNAVQWSPSHAHLLASAAMD+TICIWN+WSTEQKLA
Sbjct: 121 AKNYLQQGLMPKRMSVSLDCHTKAVNAVQWSPSHAHLLASAAMDYTICIWNIWSTEQKLA 180

Query: 224 FKSSIHDAAVKNVQWSQQGLSILSCGYDCASRLIDVEKGTELGVFKEDQGVAVVKFHPNN 283
           FKSSIH+AAVK+V+WSQQGLSILSCGYDC+SRLIDVEKGTE+GVFKEDQGVAVVKFHPN+
Sbjct: 181 FKSSIHNAAVKDVEWSQQGLSILSCGYDCSSRLIDVEKGTEIGVFKEDQGVAVVKFHPND 240

Query: 284 PNLFLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVSASNSSES 343
           P+LFLSGG+KGSLRMWD+RIGKVVNKYNRRLGPILDVEFTPN NQFISSSDVS+SNSSES
Sbjct: 241 PSLFLSGGVKGSLRMWDVRIGKVVNKYNRRLGPILDVEFTPNVNQFISSSDVSSSNSSES 300

Query: 344 SIIVWDVTREVPLSYQ 360
           S+IVWDVTREVPLSYQ
Sbjct: 301 SVIVWDVTREVPLSYQ 313

BLAST of Sgr020054 vs. ExPASy TrEMBL
Match: A0A1S4DZ03 (WD repeat-containing protein 25 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493399 PE=4 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 2.1e-144
Identity = 265/316 (83.86%), Postives = 284/316 (89.87%), Query Frame = 0

Query: 44  MESLFEAYSNASDDDDEEEEEPKPKRLALPSSSSKRPKPERSKFLPKFQSSHCPDLNSEP 103
           MESLF+AYSN+SDD D EE+EPKPKRLAL SS SKRP+ E  KF P  + SHC   N +P
Sbjct: 1   MESLFKAYSNSSDDYD-EEDEPKPKRLALSSSPSKRPELEPFKFKPNIRPSHCSHSNPQP 60

Query: 104 QKGIMVPGRYVSKRERALLSSAPVSRAPDSFLNPSVPSSTVGSLSDSDLPCDIVSLLRSR 163
            + IMVPGRY+SKRERALLSSAP SRAP+SF NPS+ S  VGSLSDSDLP DI+SLLRSR
Sbjct: 61  HERIMVPGRYISKRERALLSSAPASRAPESFPNPSLQSPPVGSLSDSDLPRDILSLLRSR 120

Query: 164 AKDYLQQCLMPERMSVTLDCHTKAVNAVQWSPSHAHLLASAAMDHTICIWNVWSTEQKLA 223
            KDYLQQ LMP+RMSVTLDCH KAVN+VQWSPSHAHLLASAAMDHTICIWNVWST QKLA
Sbjct: 121 GKDYLQQGLMPKRMSVTLDCHRKAVNSVQWSPSHAHLLASAAMDHTICIWNVWSTGQKLA 180

Query: 224 FKSSIHDAAVKNVQWSQQGLSILSCGYDCASRLIDVEKGTELGVFKEDQGVAVVKFHPNN 283
            KSS H+AAVK+VQWSQQGLS+LSCGYDCASRLIDVEKGTE+GVFKEDQGVAVVKFHPNN
Sbjct: 181 LKSSFHNAAVKDVQWSQQGLSVLSCGYDCASRLIDVEKGTEVGVFKEDQGVAVVKFHPNN 240

Query: 284 PNLFLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVSASNSSES 343
           PNLFLSGGLKGSLRMWDMRIGK+VNKYNRRLG ILD+EFTPN NQFISSSDVS SNSSES
Sbjct: 241 PNLFLSGGLKGSLRMWDMRIGKMVNKYNRRLGSILDLEFTPNSNQFISSSDVSTSNSSES 300

Query: 344 SIIVWDVTREVPLSYQ 360
           SIIVWDVTREVPLSYQ
Sbjct: 301 SIIVWDVTREVPLSYQ 315

BLAST of Sgr020054 vs. ExPASy TrEMBL
Match: A0A1S4DZQ2 (WD repeat-containing protein 25 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493399 PE=4 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 2.1e-144
Identity = 265/316 (83.86%), Postives = 284/316 (89.87%), Query Frame = 0

Query: 44  MESLFEAYSNASDDDDEEEEEPKPKRLALPSSSSKRPKPERSKFLPKFQSSHCPDLNSEP 103
           MESLF+AYSN+SDD D EE+EPKPKRLAL SS SKRP+ E  KF P  + SHC   N +P
Sbjct: 1   MESLFKAYSNSSDDYD-EEDEPKPKRLALSSSPSKRPELEPFKFKPNIRPSHCSHSNPQP 60

Query: 104 QKGIMVPGRYVSKRERALLSSAPVSRAPDSFLNPSVPSSTVGSLSDSDLPCDIVSLLRSR 163
            + IMVPGRY+SKRERALLSSAP SRAP+SF NPS+ S  VGSLSDSDLP DI+SLLRSR
Sbjct: 61  HERIMVPGRYISKRERALLSSAPASRAPESFPNPSLQSPPVGSLSDSDLPRDILSLLRSR 120

Query: 164 AKDYLQQCLMPERMSVTLDCHTKAVNAVQWSPSHAHLLASAAMDHTICIWNVWSTEQKLA 223
            KDYLQQ LMP+RMSVTLDCH KAVN+VQWSPSHAHLLASAAMDHTICIWNVWST QKLA
Sbjct: 121 GKDYLQQGLMPKRMSVTLDCHRKAVNSVQWSPSHAHLLASAAMDHTICIWNVWSTGQKLA 180

Query: 224 FKSSIHDAAVKNVQWSQQGLSILSCGYDCASRLIDVEKGTELGVFKEDQGVAVVKFHPNN 283
            KSS H+AAVK+VQWSQQGLS+LSCGYDCASRLIDVEKGTE+GVFKEDQGVAVVKFHPNN
Sbjct: 181 LKSSFHNAAVKDVQWSQQGLSVLSCGYDCASRLIDVEKGTEVGVFKEDQGVAVVKFHPNN 240

Query: 284 PNLFLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVSASNSSES 343
           PNLFLSGGLKGSLRMWDMRIGK+VNKYNRRLG ILD+EFTPN NQFISSSDVS SNSSES
Sbjct: 241 PNLFLSGGLKGSLRMWDMRIGKMVNKYNRRLGSILDLEFTPNSNQFISSSDVSTSNSSES 300

Query: 344 SIIVWDVTREVPLSYQ 360
           SIIVWDVTREVPLSYQ
Sbjct: 301 SIIVWDVTREVPLSYQ 315

BLAST of Sgr020054 vs. TAIR 10
Match: AT5G54520.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 298.9 bits (764), Expect = 5.5e-81
Identity = 170/334 (50.90%), Postives = 214/334 (64.07%), Query Frame = 0

Query: 44  MESLFEAYSNASDDDDE--EEEEPKPKRLALPSSSSKRP---------KPERSKFLP--K 103
           M+ +  +Y+N SDD+ E    E    K  A PS  SKRP         KP R  + P   
Sbjct: 1   MDLICNSYANDSDDEPEPVTNERLTSKIAAAPSIPSKRPYPVPEERQYKPPRRPYPPHGS 60

Query: 104 FQSSHCPDLNSEP-QKGIMVPGRYVSKRERALLSSAPVSRAPDSFLN----PSVPSSTVG 163
           +  S      S P    + VPGRYVSKRER+LL+S      PD   +    P    + +G
Sbjct: 61  YSESQTSSSFSIPVPVPVPVPGRYVSKRERSLLASLSTIPTPDQSSDLSQKPYSSPTVLG 120

Query: 164 SLSDSDLPCDIVSLLRSRAKDYLQQCLMPERMSVTLDCHTKAVNAVQWSPSHAHLLASAA 223
           S+SDSD+P  ++S +R R K    Q  MP RMS++L  HTKAV A+ WS SH HLLASA 
Sbjct: 121 SISDSDVPRHVLSSVRHRPKGSSLQTEMPSRMSISLTGHTKAVTAIDWSTSHVHLLASAG 180

Query: 224 MDHTICIWNVWSTEQKLAFKSSIHDAAVKNVQWSQQGLSILSCGYDCASRLIDVEKGTEL 283
           +D  + +WNVWS ++K       H+A VK+V+WS+QGLS+LSCGYDC SRL DVE+G E 
Sbjct: 181 LDGAVYVWNVWSNDKKKVRAFLHHNAPVKDVKWSKQGLSLLSCGYDCTSRLFDVERGVET 240

Query: 284 GVFKEDQGVAVVKFHPNNPNLFLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTPN 343
             FKED+ V VVKFHP+N N+FLSGG KGSLR+WD+R  K V++Y R LGPILDVEF   
Sbjct: 241 QSFKEDEVVGVVKFHPDNCNVFLSGGSKGSLRLWDIRANKFVHEYVRDLGPILDVEFIAG 300

Query: 344 GNQFISSSDVSASNSSESSIIVWDVTREVPLSYQ 360
           G QFISSSDVS  N SE+++IVWD++REVPLS Q
Sbjct: 301 GKQFISSSDVSGRNISENAVIVWDISREVPLSNQ 334

BLAST of Sgr020054 vs. TAIR 10
Match: AT1G10580.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 106.7 bits (265), Expect = 4.0e-23
Identity = 60/197 (30.46%), Postives = 102/197 (51.78%), Query Frame = 0

Query: 164 AKDYLQQCLMPERMSVTLDCHTKAVNAVQWSPSHAHLLASAAMDHTICIWNVWSTEQKLA 223
           AK     C +P+R+  T   HTK V+A+++ P   HLL SA MD  + IW+V+++ + + 
Sbjct: 261 AKANNDHCYIPKRLVHTWSGHTKGVSAIRFFPKQGHLLLSAGMDCKVKIWDVYNSGKCMR 320

Query: 224 FKSSIHDAAVKNVQWSQQGLSILSCGYDCASRLIDVEKGTELGVFKEDQGVAVVKFHPNN 283
                H  AV+++ +S  G   L+ GYD   +  D E G  +  F   +   VVK +P++
Sbjct: 321 TYMG-HAKAVRDICFSNDGSKFLTAGYDKNIKYWDTETGQVISTFSTGKIPYVVKLNPDD 380

Query: 284 --PNLFLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVSASNSS 343
              N+ L+G     +  WD+  G+V  +Y++ LG +  + F  N  +F++SSD       
Sbjct: 381 DKQNILLAGMSDKKIVQWDINTGEVTQEYDQHLGAVNTITFVDNNRRFVTSSD------- 440

Query: 344 ESSIIVWDVTREVPLSY 359
           + S+ VW+    V + Y
Sbjct: 441 DKSLRVWEFGIPVVIKY 449

BLAST of Sgr020054 vs. TAIR 10
Match: AT5G67320.1 (WD-40 repeat family protein )

HSP 1 Score: 67.8 bits (164), Expect = 2.1e-11
Identity = 53/183 (28.96%), Postives = 78/183 (42.62%), Query Frame = 0

Query: 157 VSLLRSRAKDYLQQCLMPE-RMSVTLDCHTKAVNAVQWSPSHAHLLASAAMDHTICIWNV 216
           VS   S     +  C + E R + T   H   VN V+W P+   LLAS + D T  IWN+
Sbjct: 419 VSFATSSTDSMIYLCKIGETRPAKTFTGHQGEVNCVKWDPT-GSLLASCSDDSTAKIWNI 478

Query: 217 WSTEQKLAFKSSIHDAAVKNVQWSQQG---------LSILSCGYDCASRLIDVEKGTELG 276
              +         H   +  ++WS  G         L++ S  +D   +L D E G  L 
Sbjct: 479 --KQSTFVHDLREHTKEIYTIRWSPTGPGTNNPNKQLTLASASFDSTVKLWDAELGKMLC 538

Query: 277 VFK-EDQGVAVVKFHPNNPNLFLSGGLKGSLRMWDMRIGKVVNKYNRRLGPILDVEFTPN 329
            F    + V  + F PN      SG L  S+ +W ++ GK+V  Y    G I +V +   
Sbjct: 539 SFNGHREPVYSLAFSPNG-EYIASGSLDKSIHIWSIKEGKIVKTYTGN-GGIFEVCWNKE 596

BLAST of Sgr020054 vs. TAIR 10
Match: AT1G61210.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 1.1e-09
Identity = 41/168 (24.40%), Postives = 84/168 (50.00%), Query Frame = 0

Query: 184 HTKAVNAVQWSPSHAHLLASAAMDHTICIWNVWSTEQKLAFKSSIHDAAVKNVQWSQQGL 243
           HT AV++V +  +   +LA A+    I +W+V   +   AF    H +    V++   G 
Sbjct: 57  HTSAVDSVAFDSAEVLVLAGAS-SGVIKLWDVEEAKMVRAFTG--HRSNCSAVEFHPFGE 116

Query: 244 SILSCGYDCASRLIDVEKGTELGVFK-EDQGVAVVKFHPNNPNLFLSGGLKGSLRMWDMR 303
            + S   D   ++ D+ K   +  +K   +G++ ++F P+     +SGGL   +++WD+ 
Sbjct: 117 FLASGSSDANLKIWDIRKKGCIQTYKGHSRGISTIRFTPDG-RWVVSGGLDNVVKVWDLT 176

Query: 304 IGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVSASNSSESSIIVWDV 351
            GK+++++    GPI  ++F P          + A+ S++ ++  WD+
Sbjct: 177 AGKLLHEFKFHEGPIRSLDFHP-------LEFLLATGSADRTVKFWDL 213

BLAST of Sgr020054 vs. TAIR 10
Match: AT1G61210.2 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 1.1e-09
Identity = 41/168 (24.40%), Postives = 84/168 (50.00%), Query Frame = 0

Query: 184 HTKAVNAVQWSPSHAHLLASAAMDHTICIWNVWSTEQKLAFKSSIHDAAVKNVQWSQQGL 243
           HT AV++V +  +   +LA A+    I +W+V   +   AF    H +    V++   G 
Sbjct: 57  HTSAVDSVAFDSAEVLVLAGAS-SGVIKLWDVEEAKMVRAFTG--HRSNCSAVEFHPFGE 116

Query: 244 SILSCGYDCASRLIDVEKGTELGVFK-EDQGVAVVKFHPNNPNLFLSGGLKGSLRMWDMR 303
            + S   D   ++ D+ K   +  +K   +G++ ++F P+     +SGGL   +++WD+ 
Sbjct: 117 FLASGSSDANLKIWDIRKKGCIQTYKGHSRGISTIRFTPDG-RWVVSGGLDNVVKVWDLT 176

Query: 304 IGKVVNKYNRRLGPILDVEFTPNGNQFISSSDVSASNSSESSIIVWDV 351
            GK+++++    GPI  ++F P          + A+ S++ ++  WD+
Sbjct: 177 AGKLLHEFKFHEGPIRSLDFHP-------LEFLLATGSADRTVKFWDL 213