Sgr019858 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr019858
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	rRNA N-glycosidase
Location	tig00153419: 1126716 .. 1127624 (-)
RNA-Seq Expression	Sgr019858
Synteny	Sgr019858

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCAAATTCTTAGTGCTTTCTTTACTAATTCTCACCATCATCCTTGGAGCTCCTACTGCTGAGGGCGATATCAGCTTCCAATTGTCAGGTGCTACTACCAAATCTTATGGAGATTTCATCACAAATTTTAGAAGCGCTCTTCCATTTAAGGAGGAAATATACCGTATACCTTTGTTGCTTCCTGCTGTTTCGAGCTCACAACGCTACACTCTAATCCATCTCACCAATTATGCGTATGAAACCATCACAGTGGCTATAGATGTAACAAACGTTTATGTTATGGGGTATCGCACTGGTGATGTATGCTACTTTTTTAATGAGCCTTCTGCTATTGAAGCTTCTAAATTTGTATTCAAAGATACTAAGCAGAAAATTATACTTCCATATTCTGGAAATTATGAAAGGCTTCAAACTGCTGCAGGCAAAATAAGAGAAAATATTTACCTTGGACTCCCAGCCTTGGACAGTGCAATTACCACGTTGTTTTACTACAACTCCAGATCTGCTGCAGCGGCATTTCTCGTACTCATTCAGACGACTGCTGAGGCTGCAAGATTCAAATTTATTGAGCAACAAATTGGCAAGCGTGTTGACAAAAACTTTCTACCAAGCCTAGCAATTATAAGTTTAGAAAATAGCTGGTCTGCTCTCTCCAAACAAATTCAGATAGCCAAGAATAATAAAGGACAATTTGAAACTCCCGTTGTGCTTATAAATGGTAAAGGTGAGCGAGTCGAAATAACCAATGTTAGTGCAGGAGTTGTAACCTCCAACATCGCATTACTGCTAAACAGAAACAATGTTGCAGCTGCTGACAATGGTATTCTTGCGACGTCGAGACTTGGAGCTACAAGAATGCCTATCGTCATTAACGTCAATGGATATGGAAGTTATGTTACGGTGTAG

mRNA sequence

ATGAGCAAATTCTTAGTGCTTTCTTTACTAATTCTCACCATCATCCTTGGAGCTCCTACTGCTGAGGGCGATATCAGCTTCCAATTGTCAGGTGCTACTACCAAATCTTATGGAGATTTCATCACAAATTTTAGAAGCGCTCTTCCATTTAAGGAGGAAATATACCGTATACCTTTGTTGCTTCCTGCTGTTTCGAGCTCACAACGCTACACTCTAATCCATCTCACCAATTATGCGTATGAAACCATCACAGTGGCTATAGATGTAACAAACGTTTATGTTATGGGGTATCGCACTGGTGATGTATGCTACTTTTTTAATGAGCCTTCTGCTATTGAAGCTTCTAAATTTGTATTCAAAGATACTAAGCAGAAAATTATACTTCCATATTCTGGAAATTATGAAAGGCTTCAAACTGCTGCAGGCAAAATAAGAGAAAATATTTACCTTGGACTCCCAGCCTTGGACAGTGCAATTACCACGTTGTTTTACTACAACTCCAGATCTGCTGCAGCGGCATTTCTCGTACTCATTCAGACGACTGCTGAGGCTGCAAGATTCAAATTTATTGAGCAACAAATTGGCAAGCGTGTTGACAAAAACTTTCTACCAAGCCTAGCAATTATAAGTTTAGAAAATAGCTGGTCTGCTCTCTCCAAACAAATTCAGATAGCCAAGAATAATAAAGGACAATTTGAAACTCCCGTTGTGCTTATAAATGGTAAAGGTGAGCGAGTCGAAATAACCAATGTTAGTGCAGGAGTTGTAACCTCCAACATCGCATTACTGCTAAACAGAAACAATGTTGCAGCTGCTGACAATGGTATTCTTGCGACGTCGAGACTTGGAGCTACAAGAATGCCTATCGTCATTAACGTCAATGGATATGGAAGTTATGTTACGGTGTAG

Coding sequence (CDS)

ATGAGCAAATTCTTAGTGCTTTCTTTACTAATTCTCACCATCATCCTTGGAGCTCCTACTGCTGAGGGCGATATCAGCTTCCAATTGTCAGGTGCTACTACCAAATCTTATGGAGATTTCATCACAAATTTTAGAAGCGCTCTTCCATTTAAGGAGGAAATATACCGTATACCTTTGTTGCTTCCTGCTGTTTCGAGCTCACAACGCTACACTCTAATCCATCTCACCAATTATGCGTATGAAACCATCACAGTGGCTATAGATGTAACAAACGTTTATGTTATGGGGTATCGCACTGGTGATGTATGCTACTTTTTTAATGAGCCTTCTGCTATTGAAGCTTCTAAATTTGTATTCAAAGATACTAAGCAGAAAATTATACTTCCATATTCTGGAAATTATGAAAGGCTTCAAACTGCTGCAGGCAAAATAAGAGAAAATATTTACCTTGGACTCCCAGCCTTGGACAGTGCAATTACCACGTTGTTTTACTACAACTCCAGATCTGCTGCAGCGGCATTTCTCGTACTCATTCAGACGACTGCTGAGGCTGCAAGATTCAAATTTATTGAGCAACAAATTGGCAAGCGTGTTGACAAAAACTTTCTACCAAGCCTAGCAATTATAAGTTTAGAAAATAGCTGGTCTGCTCTCTCCAAACAAATTCAGATAGCCAAGAATAATAAAGGACAATTTGAAACTCCCGTTGTGCTTATAAATGGTAAAGGTGAGCGAGTCGAAATAACCAATGTTAGTGCAGGAGTTGTAACCTCCAACATCGCATTACTGCTAAACAGAAACAATGTTGCAGCTGCTGACAATGGTATTCTTGCGACGTCGAGACTTGGAGCTACAAGAATGCCTATCGTCATTAACGTCAATGGATATGGAAGTTATGTTACGGTGTAG

Protein sequence

MSKFLVLSLLILTIILGAPTAEGDISFQLSGATTKSYGDFITNFRSALPFKEEIYRIPLLLPAVSSSQRYTLIHLTNYAYETITVAIDVTNVYVMGYRTGDVCYFFNEPSAIEASKFVFKDTKQKIILPYSGNYERLQTAAGKIRENIYLGLPALDSAITTLFYYNSRSAAAAFLVLIQTTAEAARFKFIEQQIGKRVDKNFLPSLAIISLENSWSALSKQIQIAKNNKGQFETPVVLINGKGERVEITNVSAGVVTSNIALLLNRNNVAAADNGILATSRLGATRMPIVINVNGYGSYVTV

Homology

[Close]

BLAST of Sgr019858 vs. NCBI nr
Match: P24478.2 (RecName: Full=Ribosome-inactivating protein karasurin-C; AltName: Full=rRNA N-glycosidase; Contains: RecName: Full=Ribosome-inactivating protein karasurin-A; Flags: Precursor [Trichosanthes kirilowii] >BAA21786.1 karasurin precursor [Trichosanthes kirilowii var. japonica])

HSP 1 Score: 403.3 bits (1035), Expect = 1.9e-108
Identity = 210/283 (74.20%), Postives = 237/283 (83.75%), Query Frame = 0


Query: 1   MSKFLVLSLLILTIILGAPTAEGDISFQLSGATTKSYGDFITNFRSALPFKEEIYRIPLL 60
           M +FLV SLLILT+ L AP  EGD+SF+LSGAT+ SYG FI+N R ALP++ ++Y IPLL
Sbjct: 1   MIRFLVFSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPYERKLYDIPLL 60

Query: 61  LPAVSSSQRYTLIHLTNYAYETITVAIDVTNVYVMGYRTGDVCYFFNEPSAIEASKFVFK 120
              +  SQRY LIHLTNYA ETI+VAIDVTNVYVMGYR GD  YFFNE SA EA+K+VFK
Sbjct: 61  RSTLPGSQRYALIHLTNYADETISVAIDVTNVYVMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 DTKQKIILPYSGNYERLQTAAGKIRENIYLGLPALDSAITTLFYYNSRSAAAAFLVLIQT 180
           D K+K+ LPYSGNYERLQ AAGKIRENI LGLPALDSAITTLFYYN+ SAA+A +VLIQ+
Sbjct: 121 DAKRKVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TAEAARFKFIEQQIGKRVDKNFLPSLAIISLENSWSALSKQIQIAKNNKGQFETPVVLIN 240
           T+EAAR+KFIEQQIGKRVDK FLPSLAIISLENSWSALSKQIQIA  N GQFETPVVLIN
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFETPVVLIN 240

Query: 241 GKGERVEITNVSAGVVTSNIALLLNRNNVAAADNGILATSRLG 284
            + +RV ITNV AGVVTSNIALLLNRNN+AA D+ +      G
Sbjct: 241 AQNQRVTITNVDAGVVTSNIALLLNRNNMAAIDDDVPMAQSFG 283

[Close]

BLAST of Sgr019858 vs. NCBI nr
Match: AAA34206.1 (trichosanthin [Trichosanthes kirilowii])

HSP 1 Score: 402.5 bits (1033), Expect = 3.2e-108
Identity = 210/283 (74.20%), Postives = 238/283 (84.10%), Query Frame = 0


Query: 1   MSKFLVLSLLILTIILGAPTAEGDISFQLSGATTKSYGDFITNFRSALPFKEEIYRIPLL 60
           M +FLVLSLLILT+ L  P  EGD+SF+LSGAT+ SYG FI+N R ALP + ++Y IPLL
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  LPAVSSSQRYTLIHLTNYAYETITVAIDVTNVYVMGYRTGDVCYFFNEPSAIEASKFVFK 120
             ++  SQRY LIHLTNYA ETI+VAIDVTNVY+MGYR GD  YFFNE SA EA+K+VFK
Sbjct: 61  RSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 DTKQKIILPYSGNYERLQTAAGKIRENIYLGLPALDSAITTLFYYNSRSAAAAFLVLIQT 180
           D  +K+ LPYSGNYERLQTAAGKIRENI LGLPALDSAITTLFYYN+ SAA+A +VLIQ+
Sbjct: 121 DAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TAEAARFKFIEQQIGKRVDKNFLPSLAIISLENSWSALSKQIQIAKNNKGQFETPVVLIN 240
           T+EAAR+KFIEQQIGKRVDK FLPSLAIISLENSWSALSKQIQIA  N GQFETPVVLIN
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFETPVVLIN 240

Query: 241 GKGERVEITNVSAGVVTSNIALLLNRNNVAAADNGILATSRLG 284
            + +RV ITNV AGVVTSNIALLLNRNN+AA D+ +  T   G
Sbjct: 241 AQNQRVMITNVDAGVVTSNIALLLNRNNMAAMDDDVPMTQSFG 283

[Close]

BLAST of Sgr019858 vs. NCBI nr
Match: AAK52960.1 (trichosanthin precursor [Trichosanthes kirilowii])

HSP 1 Score: 402.1 bits (1032), Expect = 4.1e-108
Identity = 210/283 (74.20%), Postives = 239/283 (84.45%), Query Frame = 0


Query: 1   MSKFLVLSLLILTIILGAPTAEGDISFQLSGATTKSYGDFITNFRSALPFKEEIYRIPLL 60
           M +FLVLSLLILT+ L  P  EGD+SF+LSGAT+ SYG FI+N R ALP + ++Y IPLL
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  LPAVSSSQRYTLIHLTNYAYETITVAIDVTNVYVMGYRTGDVCYFFNEPSAIEASKFVFK 120
             ++  SQRY LIHLTNYA ETI+VAIDVTNVY+MGYR GD  YFFNE SA EA+K+VFK
Sbjct: 61  RSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 DTKQKIILPYSGNYERLQTAAGKIRENIYLGLPALDSAITTLFYYNSRSAAAAFLVLIQT 180
           D+ +KI LPYSGNYERLQTAAGKIRENI LGLPALDSAITTLFYYN+ SAA+A +VLIQ+
Sbjct: 121 DSMRKITLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TAEAARFKFIEQQIGKRVDKNFLPSLAIISLENSWSALSKQIQIAKNNKGQFETPVVLIN 240
           T+EAAR+KFIEQQIGKRVDK FLPSLAIISLENSWSALSKQIQIA  N GQFE+PVVLIN
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLIN 240

Query: 241 GKGERVEITNVSAGVVTSNIALLLNRNNVAAADNGILATSRLG 284
            + +RV ITNV AGVVTSNIALLLNRNN+AA D+ +  T   G
Sbjct: 241 AQNQRVTITNVDAGVVTSNIALLLNRNNMAAMDDDVPMTQSFG 283

[Close]

BLAST of Sgr019858 vs. NCBI nr
Match: P09989.2 (RecName: Full=Ribosome-inactivating protein alpha-trichosanthin; Short=Alpha-TCS; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Trichosanthes kirilowii] >AAA34207.1 trichosanthin precursor [Trichosanthes kirilowii])

HSP 1 Score: 401.4 bits (1030), Expect = 7.1e-108
Identity = 209/283 (73.85%), Postives = 238/283 (84.10%), Query Frame = 0


Query: 1   MSKFLVLSLLILTIILGAPTAEGDISFQLSGATTKSYGDFITNFRSALPFKEEIYRIPLL 60
           M +FLVLSLLILT+ L  P  EGD+SF+LSGAT+ SYG FI+N R ALP + ++Y IPLL
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  LPAVSSSQRYTLIHLTNYAYETITVAIDVTNVYVMGYRTGDVCYFFNEPSAIEASKFVFK 120
             ++  SQRY LIHLTNYA ETI+VAIDVTNVY+MGYR GD  YFFNE SA EA+K+VFK
Sbjct: 61  RSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 DTKQKIILPYSGNYERLQTAAGKIRENIYLGLPALDSAITTLFYYNSRSAAAAFLVLIQT 180
           D  +K+ LPYSGNYERLQTAAGKIRENI LGLPALDSAITTLFYYN+ SAA+A +VLIQ+
Sbjct: 121 DAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TAEAARFKFIEQQIGKRVDKNFLPSLAIISLENSWSALSKQIQIAKNNKGQFETPVVLIN 240
           T+EAAR+KFIEQQIGKRVDK FLPSLAIISLENSWSALSKQIQIA  N GQFE+PVVLIN
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLIN 240

Query: 241 GKGERVEITNVSAGVVTSNIALLLNRNNVAAADNGILATSRLG 284
            + +RV ITNV AGVVTSNIALLLNRNN+AA D+ +  T   G
Sbjct: 241 AQNQRVTITNVDAGVVTSNIALLLNRNNMAAMDDDVPMTQSFG 283

[Close]

BLAST of Sgr019858 vs. NCBI nr
Match: AAO72728.1 (trichosanthin precursor [Trichosanthes kirilowii])

HSP 1 Score: 401.0 bits (1029), Expect = 9.2e-108
Identity = 209/283 (73.85%), Postives = 237/283 (83.75%), Query Frame = 0


Query: 1   MSKFLVLSLLILTIILGAPTAEGDISFQLSGATTKSYGDFITNFRSALPFKEEIYRIPLL 60
           M +FLVLSLLILT+ L  P  EGD+SF+LSGAT+ SYG FI+N R ALP + ++Y IPLL
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  LPAVSSSQRYTLIHLTNYAYETITVAIDVTNVYVMGYRTGDVCYFFNEPSAIEASKFVFK 120
              +  SQRY LIHLTNYA ETI+VAIDVTNVY+MGYR GD  YFFNE SA EA+K+VFK
Sbjct: 61  RSTLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 DTKQKIILPYSGNYERLQTAAGKIRENIYLGLPALDSAITTLFYYNSRSAAAAFLVLIQT 180
           D  +K+ LPYSGNYERLQTAAGKIRENI LGLPALDSAITTLFYYN+ SAA+A +VLIQ+
Sbjct: 121 DAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TAEAARFKFIEQQIGKRVDKNFLPSLAIISLENSWSALSKQIQIAKNNKGQFETPVVLIN 240
           T+EAAR+KFIEQQIGKRVDK FLPSLAIISLENSWSALSKQIQIA  N GQFE+PVVLIN
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLIN 240

Query: 241 GKGERVEITNVSAGVVTSNIALLLNRNNVAAADNGILATSRLG 284
            + +RV ITNV AGVVTSNIALLLNRNN+AA D+ +  T   G
Sbjct: 241 AQNQRVTITNVDAGVVTSNIALLLNRNNMAAMDDDVPMTQSFG 283

[Close]

BLAST of Sgr019858 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 403.3 bits (1035), Expect = 2.4e-111
Identity = 210/283 (74.20%), Postives = 237/283 (83.75%), Query Frame = 0


Query: 1   MSKFLVLSLLILTIILGAPTAEGDISFQLSGATTKSYGDFITNFRSALPFKEEIYRIPLL 60
           M +FLV SLLILT+ L AP  EGD+SF+LSGAT+ SYG FI+N R ALP++ ++Y IPLL
Sbjct: 1   MIRFLVFSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPYERKLYDIPLL 60

Query: 61  LPAVSSSQRYTLIHLTNYAYETITVAIDVTNVYVMGYRTGDVCYFFNEPSAIEASKFVFK 120
              +  SQRY LIHLTNYA ETI+VAIDVTNVYVMGYR GD  YFFNE SA EA+K+VFK
Sbjct: 61  RSTLPGSQRYALIHLTNYADETISVAIDVTNVYVMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 DTKQKIILPYSGNYERLQTAAGKIRENIYLGLPALDSAITTLFYYNSRSAAAAFLVLIQT 180
           D K+K+ LPYSGNYERLQ AAGKIRENI LGLPALDSAITTLFYYN+ SAA+A +VLIQ+
Sbjct: 121 DAKRKVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TAEAARFKFIEQQIGKRVDKNFLPSLAIISLENSWSALSKQIQIAKNNKGQFETPVVLIN 240
           T+EAAR+KFIEQQIGKRVDK FLPSLAIISLENSWSALSKQIQIA  N GQFETPVVLIN
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFETPVVLIN 240

Query: 241 GKGERVEITNVSAGVVTSNIALLLNRNNVAAADNGILATSRLG 284
            + +RV ITNV AGVVTSNIALLLNRNN+AA D+ +      G
Sbjct: 241 AQNQRVTITNVDAGVVTSNIALLLNRNNMAAIDDDVPMAQSFG 283

[Close]

BLAST of Sgr019858 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 401.4 bits (1030), Expect = 9.3e-111
Identity = 209/283 (73.85%), Postives = 238/283 (84.10%), Query Frame = 0


Query: 1   MSKFLVLSLLILTIILGAPTAEGDISFQLSGATTKSYGDFITNFRSALPFKEEIYRIPLL 60
           M +FLVLSLLILT+ L  P  EGD+SF+LSGAT+ SYG FI+N R ALP + ++Y IPLL
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  LPAVSSSQRYTLIHLTNYAYETITVAIDVTNVYVMGYRTGDVCYFFNEPSAIEASKFVFK 120
             ++  SQRY LIHLTNYA ETI+VAIDVTNVY+MGYR GD  YFFNE SA EA+K+VFK
Sbjct: 61  RSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 DTKQKIILPYSGNYERLQTAAGKIRENIYLGLPALDSAITTLFYYNSRSAAAAFLVLIQT 180
           D  +K+ LPYSGNYERLQTAAGKIRENI LGLPALDSAITTLFYYN+ SAA+A +VLIQ+
Sbjct: 121 DAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TAEAARFKFIEQQIGKRVDKNFLPSLAIISLENSWSALSKQIQIAKNNKGQFETPVVLIN 240
           T+EAAR+KFIEQQIGKRVDK FLPSLAIISLENSWSALSKQIQIA  N GQFE+PVVLIN
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLIN 240

Query: 241 GKGERVEITNVSAGVVTSNIALLLNRNNVAAADNGILATSRLG 284
            + +RV ITNV AGVVTSNIALLLNRNN+AA D+ +  T   G
Sbjct: 241 AQNQRVTITNVDAGVVTSNIALLLNRNNMAAMDDDVPMTQSFG 283

[Close]

BLAST of Sgr019858 vs. ExPASy Swiss-Prot
Match: P33185 (Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3)

HSP 1 Score: 395.2 bits (1014), Expect = 6.6e-109
Identity = 207/279 (74.19%), Postives = 234/279 (83.87%), Query Frame = 0


Query: 1   MSKFLVLSLLILTIILGAPTAEGDISFQLSGATTKSYGDFITNFRSALPFKEEIYRIPLL 60
           M K LVL LLILTI L +PT EGD+SF+LSGATT SYG FI N R ALP++ ++Y IPLL
Sbjct: 1   MIKLLVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPYERKVYNIPLL 60

Query: 61  LPAVSSSQRYTLIHLTNYAYETITVAIDVTNVYVMGYRTGDVCYFFNEPSAIEASKFVFK 120
             ++S S RYTL+HLTNYA ETI+VA+DVTNVY+MGY  GDV YFFNE SA EA+KFVFK
Sbjct: 61  RSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGDVSYFFNEASATEAAKFVFK 120

Query: 121 DTKQKIILPYSGNYERLQTAAGKIRENIYLGLPALDSAITTLFYYNSRSAAAAFLVLIQT 180
           D K+K+ LPYSGNYERLQTAAGKIRENI LGLPALDSAITTL+YY + SAA+A LVLIQ+
Sbjct: 121 DAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYYTASSAASALLVLIQS 180

Query: 181 TAEAARFKFIEQQIGKRVDKNFLPSLAIISLENSWSALSKQIQIAKNNKGQFETPVVLIN 240
           TAE+AR+KFIEQQIGKRVDK FLPSLA ISLEN+WSALSKQIQIA  N GQFE+PVVLI+
Sbjct: 181 TAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIASTNNGQFESPVVLID 240

Query: 241 GKGERVEITNVSAGVVTSNIALLLNRNNVAAADNGILAT 280
           G  +RV ITN SA VVTSNIALLLNRNN+AA    I  T
Sbjct: 241 GNNQRVSITNASARVVTSNIALLLNRNNIAAIGEDISMT 279

[Close]

BLAST of Sgr019858 vs. ExPASy Swiss-Prot
Match: P16094 (Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 344.7 bits (883), Expect = 1.0e-93
Identity = 179/280 (63.93%), Postives = 223/280 (79.64%), Query Frame = 0


Query: 1   MSKFLVLSLLILTIILGAPTAEGDISFQLSGATTKSYGDFITNFRSALPFKEEIYRIPLL 60
           MS+F VLS LIL I LG    +GD+SF+LSGA  +SYG FI + R+ALPF+E++Y IPLL
Sbjct: 1   MSRFSVLSFLILAIFLGGSIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPLL 60

Query: 61  LPAVSSSQRYTLIHLTNYAYETITVAIDVTNVYVMGYRTGDVCYFFNEPSAIEASKFVFK 120
           LP+VS + RY L+HL NY  +TITVA+DVTNVY+MGY      YFFNEP+A  AS++VF+
Sbjct: 61  LPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVFR 120

Query: 121 DTKQKIILPYSGNYERLQTAAGKIRENIYLGLPALDSAITTLFYYNSRSAAAAFLVLIQT 180
           D ++KI LPYSGNYERLQ AAGK RE I +GLPALDSAI+TL +Y+S +AA A LVLIQT
Sbjct: 121 DARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQT 180

Query: 181 TAEAARFKFIEQQIGKRVDKNFLPSLAIISLENSWSALSKQIQIAKNNKGQFETPVVLIN 240
           TAEAARFK+IEQQI +R  ++ +PSLA ISLENSWS LSKQIQ+A+ N G F TP+VL++
Sbjct: 181 TAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLVD 240

Query: 241 GKGERVEITNVSAGVVTSNIALLLNRNNVAAADNGILATS 281
            KG RV+ITNV++ VVTSNI LLLN  N+A  DNG ++T+
Sbjct: 241 NKGNRVQITNVTSKVVTSNIQLLLNTRNIAEGDNGDVSTT 280

[Close]

BLAST of Sgr019858 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 313.9 bits (803), Expect = 1.9e-84
Identity = 173/283 (61.13%), Postives = 210/283 (74.20%), Query Frame = 0


Query: 1   MSKFLVLSLLILTIILGAPTAEGDISFQLSGATTKSYGDFITNFRSALPFKEEIYRIPLL 60
           M K L+LS LI+ I +G PTA+GD++F LS AT K+Y  FI +FR+ LPF  ++Y IPLL
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  LPAVSSSQRYTLIHLTNYAYETITVAIDVTNVYVMGYRTGDVCYFFNEPSAIEASKFVFK 120
              +S S+R+ L++LT+YAYETI+VAIDVTNVYV+ YRT DV YFF E S  EA   +FK
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKE-SPPEAYNILFK 120

Query: 121 DTKQKIILPYSGNYERLQTAAGKIRENIYLGLPALDSAITTLFYYNSRSAAAAFLVLIQT 180
            T+ KI LPY+GNYE LQTAA KIRENI LGLPAL SAITTLFYYN++SA +A LVLIQT
Sbjct: 121 GTR-KITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQT 180

Query: 181 TAEAARFKFIEQQIGKRVDKNFLPSLAIISLENSWSALSKQIQIAKNNKGQFETPVVLIN 240
           TAEAARFK+IE+ + K V  NF P+LAIISLEN WSALSKQI +A+N  G+F  PV LI 
Sbjct: 181 TAEAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIK 240

Query: 241 GKGERVEITNVSAGVVTSNIALLLNRNNVAAADNGILATSRLG 284
             GER ++TNV + VV  NI LLLN     A +N I   + LG
Sbjct: 241 PTGERFQVTNVDSDVVKGNIKLLLNSRASTADENFITTMTLLG 281

[Close]

BLAST of Sgr019858 vs. ExPASy TrEMBL
Match: Q94KE4 (rRNA N-glycosidase OS=Trichosanthes kirilowii OX=3677 GN=TCS PE=3 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 2.0e-108
Identity = 210/283 (74.20%), Postives = 239/283 (84.45%), Query Frame = 0


Query: 1   MSKFLVLSLLILTIILGAPTAEGDISFQLSGATTKSYGDFITNFRSALPFKEEIYRIPLL 60
           M +FLVLSLLILT+ L  P  EGD+SF+LSGAT+ SYG FI+N R ALP + ++Y IPLL
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  LPAVSSSQRYTLIHLTNYAYETITVAIDVTNVYVMGYRTGDVCYFFNEPSAIEASKFVFK 120
             ++  SQRY LIHLTNYA ETI+VAIDVTNVY+MGYR GD  YFFNE SA EA+K+VFK
Sbjct: 61  RSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 DTKQKIILPYSGNYERLQTAAGKIRENIYLGLPALDSAITTLFYYNSRSAAAAFLVLIQT 180
           D+ +KI LPYSGNYERLQTAAGKIRENI LGLPALDSAITTLFYYN+ SAA+A +VLIQ+
Sbjct: 121 DSMRKITLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TAEAARFKFIEQQIGKRVDKNFLPSLAIISLENSWSALSKQIQIAKNNKGQFETPVVLIN 240
           T+EAAR+KFIEQQIGKRVDK FLPSLAIISLENSWSALSKQIQIA  N GQFE+PVVLIN
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLIN 240

Query: 241 GKGERVEITNVSAGVVTSNIALLLNRNNVAAADNGILATSRLG 284
            + +RV ITNV AGVVTSNIALLLNRNN+AA D+ +  T   G
Sbjct: 241 AQNQRVTITNVDAGVVTSNIALLLNRNNMAAMDDDVPMTQSFG 283

[Close]

BLAST of Sgr019858 vs. ExPASy TrEMBL
Match: Q84SV8 (rRNA N-glycosidase OS=Trichosanthes kirilowii OX=3677 GN=TCS PE=3 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 4.5e-108
Identity = 209/283 (73.85%), Postives = 237/283 (83.75%), Query Frame = 0


Query: 1   MSKFLVLSLLILTIILGAPTAEGDISFQLSGATTKSYGDFITNFRSALPFKEEIYRIPLL 60
           M +FLVLSLLILT+ L  P  EGD+SF+LSGAT+ SYG FI+N R ALP + ++Y IPLL
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  LPAVSSSQRYTLIHLTNYAYETITVAIDVTNVYVMGYRTGDVCYFFNEPSAIEASKFVFK 120
              +  SQRY LIHLTNYA ETI+VAIDVTNVY+MGYR GD  YFFNE SA EA+K+VFK
Sbjct: 61  RSTLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 DTKQKIILPYSGNYERLQTAAGKIRENIYLGLPALDSAITTLFYYNSRSAAAAFLVLIQT 180
           D  +K+ LPYSGNYERLQTAAGKIRENI LGLPALDSAITTLFYYN+ SAA+A +VLIQ+
Sbjct: 121 DAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TAEAARFKFIEQQIGKRVDKNFLPSLAIISLENSWSALSKQIQIAKNNKGQFETPVVLIN 240
           T+EAAR+KFIEQQIGKRVDK FLPSLAIISLENSWSALSKQIQIA  N GQFE+PVVLIN
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLIN 240

Query: 241 GKGERVEITNVSAGVVTSNIALLLNRNNVAAADNGILATSRLG 284
            + +RV ITNV AGVVTSNIALLLNRNN+AA D+ +  T   G
Sbjct: 241 AQNQRVTITNVDAGVVTSNIALLLNRNNMAAMDDDVPMTQSFG 283

[Close]

BLAST of Sgr019858 vs. ExPASy TrEMBL
Match: Q6PRG5 (rRNA N-glycosidase (Fragment) OS=Trichosanthes kirilowii OX=3677 GN=tcm PE=3 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 7.6e-108
Identity = 207/270 (76.67%), Postives = 237/270 (87.78%), Query Frame = 0


Query: 1   MSKFLVLSLLILTIILGAPTAEGDISFQLSGATTKSYGDFITNFRSALPFKEEIYRIPLL 60
           M +FLVLSLLILT+ L AP  EGD+SF+LSGAT+ SYG FI+N R ALP+++++Y IPLL
Sbjct: 1   MIRFLVLSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPYEKKLYDIPLL 60

Query: 61  LPAVSSSQRYTLIHLTNYAYETITVAIDVTNVYVMGYRTGDVCYFFNEPSAIEASKFVFK 120
             ++S SQRY LI+LTNYA ETI+VAIDVTNVY+MGYR GD+ YFFNE SA EA+K+VFK
Sbjct: 61  RSSLSGSQRYALIYLTNYADETISVAIDVTNVYIMGYRAGDISYFFNEASATEAAKYVFK 120

Query: 121 DTKQKIILPYSGNYERLQTAAGKIRENIYLGLPALDSAITTLFYYNSRSAAAAFLVLIQT 180
           D K+K+ LPYSGNYERLQ AAGKIRENI LGLPALDSAITTLFYYN+ SAA+A LVLIQ+
Sbjct: 121 DAKRKVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQS 180

Query: 181 TAEAARFKFIEQQIGKRVDKNFLPSLAIISLENSWSALSKQIQIAKNNKGQFETPVVLIN 240
           TAEAAR+KFIEQQIGKRVDK FLPSLAIISLENSWSALSKQIQIA  N GQFE+PVVLIN
Sbjct: 181 TAEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLIN 240

Query: 241 GKGERVEITNVSAGVVTSNIALLLNRNNVA 271
            + +RV I NV+AGVVTSNIALLLNRNN+A
Sbjct: 241 AQNQRVTIANVNAGVVTSNIALLLNRNNMA 270

[Close]

BLAST of Sgr019858 vs. ExPASy TrEMBL
Match: Q41216 (rRNA N-glycosidase OS=Trichosanthes kirilowii OX=3677 GN=trichosanthin: TCS PE=3 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 2.2e-107
Identity = 207/283 (73.14%), Postives = 238/283 (84.10%), Query Frame = 0


Query: 1   MSKFLVLSLLILTIILGAPTAEGDISFQLSGATTKSYGDFITNFRSALPFKEEIYRIPLL 60
           M +FLVLSLLILT+ L  P  EGD+SF+LSGAT+ SYG FI+N R ALP + ++Y IPLL
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  LPAVSSSQRYTLIHLTNYAYETITVAIDVTNVYVMGYRTGDVCYFFNEPSAIEASKFVFK 120
             ++  SQRY L+HLTNYA ETI+VAIDVT+VY+MGYR GD  YFFNE SA EA+K+VFK
Sbjct: 61  RSSLPGSQRYALVHLTNYADETISVAIDVTSVYIMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 DTKQKIILPYSGNYERLQTAAGKIRENIYLGLPALDSAITTLFYYNSRSAAAAFLVLIQT 180
           D  +K+ LPYSGNYERLQTAAGKIRENI LGLPALDSAITTLFYYN+ SAA+A +VLIQ+
Sbjct: 121 DAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TAEAARFKFIEQQIGKRVDKNFLPSLAIISLENSWSALSKQIQIAKNNKGQFETPVVLIN 240
           T+EAAR+KFIEQQIGKRVDK FLPSLAIISLENSWSALSKQIQIA  N GQFETPVVLIN
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFETPVVLIN 240

Query: 241 GKGERVEITNVSAGVVTSNIALLLNRNNVAAADNGILATSRLG 284
            + +RV ITNV AGVVTSNIALLLNRN++AA D+ +  T   G
Sbjct: 241 AQNQRVTITNVDAGVVTSNIALLLNRNDMAAMDDDVPMTQSFG 283

[Close]

BLAST of Sgr019858 vs. ExPASy TrEMBL
Match: Q8LPV7 (rRNA N-glycosidase (Fragment) OS=Trichosanthes kirilowii OX=3677 GN=TCS PE=3 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 2.2e-107
Identity = 206/270 (76.30%), Postives = 236/270 (87.41%), Query Frame = 0


Query: 1   MSKFLVLSLLILTIILGAPTAEGDISFQLSGATTKSYGDFITNFRSALPFKEEIYRIPLL 60
           M +FLVLSLLILT+ L AP  EGD+SF+LSGAT+ SYG FI+N R ALP+++++Y IPLL
Sbjct: 1   MIRFLVLSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPYEKKLYDIPLL 60

Query: 61  LPAVSSSQRYTLIHLTNYAYETITVAIDVTNVYVMGYRTGDVCYFFNEPSAIEASKFVFK 120
             ++S SQRY LI+LTNYA ETI+VAIDVTNVY+MGYR GD+ YFFNE SA EA+K+VFK
Sbjct: 61  RSSLSGSQRYALIYLTNYADETISVAIDVTNVYIMGYRAGDISYFFNEASATEAAKYVFK 120

Query: 121 DTKQKIILPYSGNYERLQTAAGKIRENIYLGLPALDSAITTLFYYNSRSAAAAFLVLIQT 180
           D K+K+ LPYSGNYERLQ AAGKIRENI LGLPALDSAITTLFYYN+ SAA+A LVLIQ+
Sbjct: 121 DAKRKVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQS 180

Query: 181 TAEAARFKFIEQQIGKRVDKNFLPSLAIISLENSWSALSKQIQIAKNNKGQFETPVVLIN 240
           TAEAAR+KFIEQQIGKRVDK FLPSLAIISLENSWSALSKQIQIA  N GQFE+PVVLIN
Sbjct: 181 TAEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLIN 240

Query: 241 GKGERVEITNVSAGVVTSNIALLLNRNNVA 271
            + +RV I NV+AGVVTSNIA LLNRNN+A
Sbjct: 241 AQNQRVTIANVNAGVVTSNIAFLLNRNNMA 270

The following BLAST results are available for this feature:

BLAST of Sgr019858 vs. NCBI nr


Match Name	E-value	Identity	Description
P24478.2	1.9e-108	74.20	RecName: Full=Ribosome-inactivating protein karasurin-C; AltName: Full=rRNA N-gl...	[more]
AAA34206.1	3.2e-108	74.20	trichosanthin [Trichosanthes kirilowii]	[more]
AAK52960.1	4.1e-108	74.20	trichosanthin precursor [Trichosanthes kirilowii]	[more]
P09989.2	7.1e-108	73.85	RecName: Full=Ribosome-inactivating protein alpha-trichosanthin; Short=Alpha-TCS...	[more]
AAO72728.1	9.2e-108	73.85	trichosanthin precursor [Trichosanthes kirilowii]	[more]

BLAST of Sgr019858 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24478	2.4e-111	74.20	Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=...	[more]
P09989	9.3e-111	73.85	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]
P33185	6.6e-109	74.19	Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3	[more]
P16094	1.0e-93	63.93	Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=...	[more]
P24817	1.9e-84	61.13	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]

BLAST of Sgr019858 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q94KE4	2.0e-108	74.20	rRNA N-glycosidase OS=Trichosanthes kirilowii OX=3677 GN=TCS PE=3 SV=1	[more]
Q84SV8	4.5e-108	73.85	rRNA N-glycosidase OS=Trichosanthes kirilowii OX=3677 GN=TCS PE=3 SV=1	[more]
Q6PRG5	7.6e-108	76.67	rRNA N-glycosidase (Fragment) OS=Trichosanthes kirilowii OX=3677 GN=tcm PE=3 SV=...	[more]
Q41216	2.2e-107	73.14	rRNA N-glycosidase OS=Trichosanthes kirilowii OX=3677 GN=trichosanthin: TCS PE=3...	[more]
Q8LPV7	2.2e-107	76.30	rRNA N-glycosidase (Fragment) OS=Trichosanthes kirilowii OX=3677 GN=TCS PE=3 SV=...	[more]

BLAST of Sgr019858 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 82..97 score: 58.55 coord: 35..48 score: 39.66 coord: 174..194 score: 60.53 coord: 206..223 score: 53.65 coord: 145..159 score: 55.96
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 24..185 e-value: 6.3E-44 score: 151.5
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 33..223 e-value: 3.6E-31 score: 108.3
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 27..268
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 186..270 e-value: 6.7E-30 score: 104.9
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 27..268
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 178..194
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 24..268

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr019858.1	Sgr019858.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
biological_process	GO:0050688	regulation of defense response to virus
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity