Sgr019432 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr019432
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	protease Do-like 5, chloroplastic
Location	tig00153347: 568603 .. 572815 (-)
RNA-Seq Expression	Sgr019432
Synteny	Sgr019432

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTTGGGCTCACTGGGAATTCGTCTCCTTCCGATTGCAGCTCCCCTATGTTCTTCAGAAAACTCCATGCCCTTCACTTCGCGAAGAGCCGTAGTGTTTGCCTCGAGTGCTTTAATGGCTTCCCTGCTCGTTTTCCCTGTTCCCACTTACGCCGCTCTTCCCCAATTACAAGATGAGGTTCCACAAGAAGAAGATCGAGTCGTTGGCCTTTTTCAGGTCTCTCTCTTTCTCAGAGGCGATAAATGTAGAATTCTTCAGCTTCAATTGAAATTGAATCTTGCTTCTTTTTCTTCTGTCTTGTTCTGTTGGCTCCAATGTAGGAAACTTCGCCTTCCGTTGTTTACATTAAGGACCTTGAAATAGCTAAAAAACCCCAGAACTCTTCTGAAGAGGCCATGTTCGTCGAGGATGAAAATGCCAAGGTCAAAGGGACAGGTTCAGGCTTTATATGGGATAAATTTGGCCATATCGTAAGCTCTTCTCTCCTTTCATTGTTACATTCCTTGATTTTGTGTACTCTTATTTTTTTTTTCCGTGGAAAATTCCATAATTTTCTCCCCGGAAGAAGTTCTCAGAGTATAAAAGTTTACACAACAGAAATGAGCATCTTTTTTTTTTTTTTTATGTCTGTCATTTTGATTTTTTTTTTATATTGGTATCAGAAACATTCATTTTGGTAACATATTTTGTCCATTCTAAGGTGGGAATTTCATTCAAGATCAGGGCTTTGTTATAACTTGTTTCCTTTTTTGAACGATATAAAGTGGCTTGTGCCAACTGTAAGTCTATATTCCTGGCTGAAAAGTTTCTACTTGTGACAGGTAACTAATTACCATGTTGTTTCAACCTTGGCTACTGATATTAGTGGATTACAGCGTTGTAAGGTTTTGTTTATTAGTTACTGTCTTTCGGATTGTTATGTCTTTTTAATGGATGATATACTGGCAGTCTGTAGCCATGATTTCAAGATAACTACTCAATTTGGCTTGCTTTTGTAGGTAAATTTAGTCGATGCTAAAGGAAATGTAATCTATAGGGAAGCAAAAATTGTAGGTTTTGATCCAGAGTATGATCTAGCTGTTCTTAAGGTATTGAACCAAGCTTAGTCTTTTTTTCTTTTTTTTTTTTGGTAATTTTCTTGGAAGCTTCTTGTCTGCATTGTCATGTGCAAACTAGGTGCTGTGAACTTCGCATAGAAGTTGAAATGGCTTCACTCAATTTCGAGGTTTATAATATCCCTCTTGAAACGGTGCTCATATACATATTCTGTTTCATTATGTGCTAATTTAGCTCGAAAGTAATGTTTTAGGCAAATATAGAAGTCCTTGTCTTTACCTGTATATGGTATTCCTGTCTTTGTTCCCTTCTAAATGACTCAAACATGTAAAAATGATTATCTTATTCTTTTAACATTATTCACAATAAGGTCGAAAAGCTACTAATTAGTGGTGTTGGAAAACGACTGATGAATTTTGTGAGAGTAAGAGCACTGTTTGTGTGTCGTATCAGAATTACATAGTCTATCACAAGTAACCTTACAGAAATTATAGTATGAGGAAGGGACTATATTTTGCTTTGGGAAGAAAGGATCTTCAAATGGCTCTAGCAGTAACTTAACAGGGAAGAAAAGCTAGATTATTCCATTTCTTTCGTTTACCTCCGGCCATAGTTTCAAGGGGAATACCAGATTGAGTGGATCATGTTTGATGTTCTTCATCCTGTTTTCCATTCCTAATGTGACGTGCAAACACGGCAGATTGGGAGGAGAGGAAAATGAACCTGTGATCTATGGAGTGATGATGATAGTATATCATTTGCTACTTCTATTCTATAAAACAGACTTTGCTGTTTGGACAATCTATACTGTCAAACTCTTATACTATACCATGATTTGCTGAGTTTGAAGTTGCCATGTAACATCCAGGTGGACCTTGAAGGACGTGAACTAAAGCCCATCGTCCTTGGTACCTCTCAAATTTACGTGTTGGTCAGAGCTGCTATGCCATTGGCAACCCTTTTGGTTATGAGAAGACACTAACAGCAGGGGTAGGACATCAATAATGTTACTTTCTCCATTAAATTTAGGGAAGCCCACTCTGTCTTTCTATTGCAAAGCTAAAACTAAATTCCGAGGTGAAAATAATTCTCTCTTATAATAAACATATTACTCATAGAAGATATGATATCTCTCATCTCACGCATTTATGTTTTAACTTTTATTTATCAAAAAATGTGGATGAAGTAAGAGTTTTTCTAAGTGGCACTATCAAGCTTCATGCTCTTAGATTGATGGCGTTCTTTACCAAGAAATACCAGTTGAGAGTCATATAGCGGATTGACTAAATCATTGAGAACCGTAAATCATTCAGGGTTTGGATAGCAGGTGATCAGTGGATTGGGTAGAGAAATTCCATCGCCAAATGGAAGGGCCATTCGGGGGGCTATTCAGACAGATGCTGCTATTAGTGCAGGTTCATGGTTCATCTACCTGAAATAAGATATTGCCATTTCAAAGACTGTTACTTTTTCATGCTTTTCTTCTTTCATCATATATATTGCAAGTGTAAGCCTTATCAATTCCATTGATCTTGATCCCATTGGGCTCGGCCAGTTAAATTCCATGTACATATATGCCAGCTTATCTTGTTCTTTCTATTCTGTCTATGTTTCGTTACTGCTCGAGTCATCCGTTATAATAATTATATTTTAACTTTCCCACATTTTAATCCATAAAAGTTTTACAGGGAATTCAGGGGGACCATTAGTTGACTCATACGGTCATGTAATTGGAGTCAACACAGCAACTTTCACTCGCAAAGGTAAGGGATCTCAATTTCTTCATCTGTCACTTGGTTTATCAGAGAAATAATGAATGCAGAAGAATGATGGCCAGTTTTGCTTTATATGTGGCTGATTTGTGTGCCATAGCTTAGTAATTCTTAAAACTCTGTAAGTTTGCATGTTCTCAAAGTTGTAGTTCTATGTGCCTTCTCATTTCAAAGTTGTACTTTTTATGTTTGGTTATGCATTGCAGGAACTGGAATGTCTTCTGGTGTTAATTTTGCAATACCAATAGACACAGTTGTACGGACCGTGCCCTACCTTATTGTATATGGAACACCTTACAGTGAGAGATTTTGATCAAGTTTTACCATCTTAAAAGGGACCACAGCCATTCATGCAACTCCAACTACTCTCCAAGACCATGGCCCACACTGTAAATGACAATTTGCTAACATAAATGAATTTGTTATTCCTCTAAGTGTATGATCCATATGCATTCTTTCAAGATCTTGATATTGATTGCGAGTAGTTATTAATGGAGTATTGCCCTTCCTTCTCTCTGTTGGGTAATTGAATTATTGACGCTACTATTGATGAACTCATTAGTGTATGCAAAGAGTTTTTGATCAACTGTTAAAACTTTCTTACCTTACATTTATATCCAAACTTGTAGCTTGCCAAACTAGAAACAATATATTGTGTCTCTGTATCCAAACACTCAGAAACAATGAAGACAAAGTATCTGGAGTGTGTTTCGATAATTTGTAAAGATTAGTGTTATTGTACACGTTAGAATTATAAACTCACGATTTTAAAAATAGAATAAACTTATTTAACAAAATATCAAGTCGAGAACACATTAAAGAGATTCAAATAAAGTCTCAAGATTTCTTCCAGATTTTTTTCATTTTTTGAGATTCCACTTGCATGCACTTGATTCAAATTAAATAAAGAAAAGGATAATATGCTCTGGTTATCCACAACCTTGCAAGCGAAAACAAATTGTATTTCCTTATATCATATTTCCACTAAAAGAACATATTGTTAAACTATTTCAATGTCTCAATAGAAACAATCATAAACATCATCTTCAAACAAGCGCCCTATAGTTTTTCTTCTCCCTTCTTAATAACCTCAGGCTTGGTCTTAATTGCTAGACTGATAGCAGCGCCAATTGTTCCTCAATGGTACTATCTACATGACAAGATGTACGGCAAGAGTAAGGGATAAGAATAAGACCGAGGCACCGTTGTTCCATTGGAGATGGCGAGCTACGTCGAGTGCTACGATACTCTACTGCATCTTGAAGAATGATATTTCCTTGCTTGTCAATGCAGTGAAACGTGCCCAAGAAATATCTTCCATCCTTAATACCTATGAGCATTCGGCGGAACAGCAGCTTTCTCACCCTCACTATGCGATCTAA

mRNA sequence

ATGGCGTTGGGCTCACTGGGAATTCGTCTCCTTCCGATTGCAGCTCCCCTATGTTCTTCAGAAAACTCCATGCCCTTCACTTCGCGAAGAGCCGTAGTGTTTGCCTCGAGTGCTTTAATGGCTTCCCTGCTCGTTTTCCCTGTTCCCACTTACGCCGCTCTTCCCCAATTACAAGATGAGGTTCCACAAGAAGAAGATCGAGTCGTTGGCCTTTTTCAGGAAACTTCGCCTTCCGTTGTTTACATTAAGGACCTTGAAATAGCTAAAAAACCCCAGAACTCTTCTGAAGAGGCCATGTTCGTCGAGGATGAAAATGCCAAGGTCAAAGGGACAGGTTCAGGCTTTATATGGGATAAATTTGGCCATATCGTAACTAATTACCATGTTGTTTCAACCTTGGCTACTGATATTAGTGGATTACAGCGTTGTAAGGTAAATTTAGTCGATGCTAAAGGAAATGTAATCTATAGGGAAGCAAAAATTGTAGGTTTTGATCCAGAGTATGATCTAGCTGTTCTTAAGGTGGACCTTGAAGGACGTGAACTAAAGCCCATCGTCCTTGGTACCTCTCAAATTTACGTGTTGGTCAGAGCTGCTATGCCATTGGCAACCCTTTTGCAGGTGATCAGTGGATTGGGTAGAGAAATTCCATCGCCAAATGGAAGGGCCATTCGGGGGGCTATTCAGACAGATGCTGCTATTAGTGCAGGGAATTCAGGGGGACCATTAGTTGACTCATACGGTCATGTAATTGGAGTCAACACAGCAACTTTCACTCGCAAAGGAACTGGAATGTCTTCTGGTGTTAATTTTGCAATACCAATAGACACAGTTCAGCGCCAATTGTTCCTCAATGGTACTATCTACATGACAAGATGTACGGCAAGAGTAAGGGATAAGAATAAGACCGAGGCACCGTTGTTCCATTGGAGATGGCGAGCTACGTCGAGTGCTACGATACTCTACTGCATCTTGAAGAATGATATTTCCTTGCTTGTCAATGCAGTGAAACGTGCCCAAGAAATATCTTCCATCCTTAATACCTATGAGCATTCGGCGGAACAGCAGCTTTCTCACCCTCACTATGCGATCTAA

Coding sequence (CDS)

ATGGCGTTGGGCTCACTGGGAATTCGTCTCCTTCCGATTGCAGCTCCCCTATGTTCTTCAGAAAACTCCATGCCCTTCACTTCGCGAAGAGCCGTAGTGTTTGCCTCGAGTGCTTTAATGGCTTCCCTGCTCGTTTTCCCTGTTCCCACTTACGCCGCTCTTCCCCAATTACAAGATGAGGTTCCACAAGAAGAAGATCGAGTCGTTGGCCTTTTTCAGGAAACTTCGCCTTCCGTTGTTTACATTAAGGACCTTGAAATAGCTAAAAAACCCCAGAACTCTTCTGAAGAGGCCATGTTCGTCGAGGATGAAAATGCCAAGGTCAAAGGGACAGGTTCAGGCTTTATATGGGATAAATTTGGCCATATCGTAACTAATTACCATGTTGTTTCAACCTTGGCTACTGATATTAGTGGATTACAGCGTTGTAAGGTAAATTTAGTCGATGCTAAAGGAAATGTAATCTATAGGGAAGCAAAAATTGTAGGTTTTGATCCAGAGTATGATCTAGCTGTTCTTAAGGTGGACCTTGAAGGACGTGAACTAAAGCCCATCGTCCTTGGTACCTCTCAAATTTACGTGTTGGTCAGAGCTGCTATGCCATTGGCAACCCTTTTGCAGGTGATCAGTGGATTGGGTAGAGAAATTCCATCGCCAAATGGAAGGGCCATTCGGGGGGCTATTCAGACAGATGCTGCTATTAGTGCAGGGAATTCAGGGGGACCATTAGTTGACTCATACGGTCATGTAATTGGAGTCAACACAGCAACTTTCACTCGCAAAGGAACTGGAATGTCTTCTGGTGTTAATTTTGCAATACCAATAGACACAGTTCAGCGCCAATTGTTCCTCAATGGTACTATCTACATGACAAGATGTACGGCAAGAGTAAGGGATAAGAATAAGACCGAGGCACCGTTGTTCCATTGGAGATGGCGAGCTACGTCGAGTGCTACGATACTCTACTGCATCTTGAAGAATGATATTTCCTTGCTTGTCAATGCAGTGAAACGTGCCCAAGAAATATCTTCCATCCTTAATACCTATGAGCATTCGGCGGAACAGCAGCTTTCTCACCCTCACTATGCGATCTAA

Protein sequence

MALGSLGIRLLPIAAPLCSSENSMPFTSRRAVVFASSALMASLLVFPVPTYAALPQLQDEVPQEEDRVVGLFQETSPSVVYIKDLEIAKKPQNSSEEAMFVEDENAKVKGTGSGFIWDKFGHIVTNYHVVSTLATDISGLQRCKVNLVDAKGNVIYREAKIVGFDPEYDLAVLKVDLEGRELKPIVLGTSQIYVLVRAAMPLATLLQVISGLGREIPSPNGRAIRGAIQTDAAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVQRQLFLNGTIYMTRCTARVRDKNKTEAPLFHWRWRATSSATILYCILKNDISLLVNAVKRAQEISSILNTYEHSAEQQLSHPHYAI

Homology

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BLAST of Sgr019432 vs. NCBI nr
Match: XP_022927238.1 (protease Do-like 5, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 441.8 bits (1135), Expect = 5.7e-120
Identity = 239/304 (78.62%), Postives = 258/304 (84.87%), Query Frame = 0


Query: 1   MALGSLGIRLLPIAAPLCSSENSMPFTSRRAVVFASSALMASLLVFPVPTYAALPQLQDE 60
           MALGSLGIRLLP+++P  SSE S+PFTSRRA+VFA +ALMASLL FPVP++AALPQLQDE
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFTSRRAIVFAPTALMASLLAFPVPSFAALPQLQDE 60

Query: 61  VPQEEDRVVGLFQETSPSVVYIKDLEIAKKPQNSSEEAMFVE-DENAKVKGTGSGFIWDK 120
           VPQEEDR+VGLFQETSPSVVYIK+LEIAKKPQN SEEAM +E DENAKVKGTGSGFIWDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVYIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 121 FGHIVTNYHVVSTLATDISGLQRCKVNLVDAKGNVIYREAKIVGFDPEYDLAVLKVDLEG 180
           FGHIVTNYHVVS LATD SGLQRCKVNLVD KGN I R+AKIVGFDPEYDLAVLKV+LEG
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELEG 180

Query: 181 RELKPIVLGTSQIYVLVRAAMPLAT--------LLQVISGLGREIPSPNGRAIRGAIQTD 240
            ELKPIV GTS+   + ++   +             VISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVQR---QLFLNGTI 293
           AAISAGNSGGPLVD YGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV R    L + GT 
Sbjct: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

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BLAST of Sgr019432 vs. NCBI nr
Match: XP_022140016.1 (protease Do-like 5, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 438.0 bits (1125), Expect = 8.2e-119
Identity = 235/303 (77.56%), Postives = 254/303 (83.83%), Query Frame = 0


Query: 1   MALGSLGIRLLPIAAPLCSSENSMPFTSRRAVVFASSALMASLLVFPVPTYAALPQLQDE 60
           MAL SLGI LLPI AP  SS NS+PFTSRRA+VFA SALMASLL FP+PT+AALPQ+Q +
Sbjct: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQIQAQ 60

Query: 61  VPQEEDRVVGLFQETSPSVVYIKDLEIAKKPQNSSEEAMFVEDENAKVKGTGSGFIWDKF 120
           VPQEEDRVV LFQ+ SPSVVYIKDLE+AKKPQNSSEEA+ VEDEN KVKGTGSGF+WDKF
Sbjct: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF 120

Query: 121 GHIVTNYHVVSTLATDISGLQRCKVNLVDAKGNVIYREAKIVGFDPEYDLAVLKVDLEGR 180
           GHIVTNYHVVS LATD SGLQRCKVNLVDAKGN IYREAKIVGFDPEYDLAVLKV+L G 
Sbjct: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGC 180

Query: 181 ELKPIVLGTSQIYVLVRAAMPLAT--------LLQVISGLGREIPSPNGRAIRGAIQTDA 240
           ELKPIVLGTS+   + ++   +             VISGLGREIPSPNGRAIRG IQTDA
Sbjct: 181 ELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGGIQTDA 240

Query: 241 AISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVQR---QLFLNGTIY 293
           AIS+GNSGGPL+DSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV R    L + GT Y
Sbjct: 241 AISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPY 300

[Close]

BLAST of Sgr019432 vs. NCBI nr
Match: XP_022140015.1 (protease Do-like 5, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 437.2 bits (1123), Expect = 1.4e-118
Identity = 231/292 (79.11%), Postives = 250/292 (85.62%), Query Frame = 0


Query: 1   MALGSLGIRLLPIAAPLCSSENSMPFTSRRAVVFASSALMASLLVFPVPTYAALPQLQDE 60
           MAL SLGI LLPI AP  SS NS+PFTSRRA+VFA SALMASLL FP+PT+AALPQ+Q +
Sbjct: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQIQAQ 60

Query: 61  VPQEEDRVVGLFQETSPSVVYIKDLEIAKKPQNSSEEAMFVEDENAKVKGTGSGFIWDKF 120
           VPQEEDRVV LFQ+ SPSVVYIKDLE+AKKPQNSSEEA+ VEDEN KVKGTGSGF+WDKF
Sbjct: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF 120

Query: 121 GHIVTNYHVVSTLATDISGLQRCKVNLVDAKGNVIYREAKIVGFDPEYDLAVLKVDLEGR 180
           GHIVTNYHVVS LATD SGLQRCKVNLVDAKGN IYREAKIVGFDPEYDLAVLKV+L G 
Sbjct: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGC 180

Query: 181 ELKPIVLGTSQIYVLVRAAMPLAT--------LLQVISGLGREIPSPNGRAIRGAIQTDA 240
           ELKPIVLGTS+   + ++   +             VISGLGREIPSPNGRAIRG IQTDA
Sbjct: 181 ELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGGIQTDA 240

Query: 241 AISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVQRQLFL 285
           AIS+GNSGGPL+DSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV  Q F+
Sbjct: 241 AISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVGTQPFM 292

[Close]

BLAST of Sgr019432 vs. NCBI nr
Match: KAG6583889.1 (Protease Do-like 5, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 436.0 bits (1120), Expect = 3.1e-118
Identity = 231/289 (79.93%), Postives = 252/289 (87.20%), Query Frame = 0


Query: 1   MALGSLGIRLLPIAAPLCSSENSMPFTSRRAVVFASSALMASLLVFPVPTYAALPQLQDE 60
           MALGSLGIRLLP+++P  SSE S+PFTSRRA+VFA +ALMASLL FPVP++AALPQLQDE
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFTSRRAIVFAPTALMASLLAFPVPSFAALPQLQDE 60

Query: 61  VPQEEDRVVGLFQETSPSVVYIKDLEIAKKPQNSSEEAMFVE-DENAKVKGTGSGFIWDK 120
           VPQEEDR+VGLFQETSPSVVYIK+LEIAKKPQN SEEAM +E DENAKVKGTGSGFIWDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVYIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 121 FGHIVTNYHVVSTLATDISGLQRCKVNLVDAKGNVIYREAKIVGFDPEYDLAVLKVDLEG 180
           FGHIVTNYHVVS LATD SGLQRCKVNLVD +GN I R+AK+VGFDPEYDLAVLKV+L+G
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVEGNGISRDAKVVGFDPEYDLAVLKVELQG 180

Query: 181 RELKPIVLGTSQIYVLVRAAMPLAT--------LLQVISGLGREIPSPNGRAIRGAIQTD 240
            ELKPIV GTS+   + ++   +             VISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVQR 281
           AAISAGNSGGPLVD YGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV R
Sbjct: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVR 289

[Close]

BLAST of Sgr019432 vs. NCBI nr
Match: XP_038893976.1 (protease Do-like 5, chloroplastic [Benincasa hispida])

HSP 1 Score: 432.6 bits (1111), Expect = 3.4e-117
Identity = 233/303 (76.90%), Postives = 252/303 (83.17%), Query Frame = 0


Query: 1   MALGSLGIRLLPIAAPLCSSENSMPFTSRRAVVFASSALMASLLVFPVPTYAALPQLQDE 60
           MALGSLGIRLLPI++P  S+EN +P TSRRA+VFA +ALMASLL FPVPTYAALPQLQD+
Sbjct: 1   MALGSLGIRLLPISSPPNSAENPLPITSRRAIVFAPTALMASLLAFPVPTYAALPQLQDD 60

Query: 61  VPQEEDRVVGLFQETSPSVVYIKDLEIAKKPQNSSEEAMFVEDENAKVKGTGSGFIWDKF 120
           +PQEEDR+V LFQETSPSVVYIKDLE+AK PQN S      EDENAKVKGTGSGF+WDKF
Sbjct: 61  IPQEEDRIVALFQETSPSVVYIKDLEVAKNPQNPSG-----EDENAKVKGTGSGFVWDKF 120

Query: 121 GHIVTNYHVVSTLATDISGLQRCKVNLVDAKGNVIYREAKIVGFDPEYDLAVLKVDLEGR 180
           GHIVTNYHVVS LATD SGLQRCKVNLVD KGN IY+EAKIVGFDPEYDLAVLKV+LEG 
Sbjct: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEGH 180

Query: 181 ELKPIVLGTSQIYVLVRAAMPLAT--------LLQVISGLGREIPSPNGRAIRGAIQTDA 240
           ELKPIV GTS+   + ++   +             VISGLGREIPSPNGRAIRGAIQTDA
Sbjct: 181 ELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDA 240

Query: 241 AISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVQR---QLFLNGTIY 293
           AISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV R    L + GT Y
Sbjct: 241 AISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVIRTVPYLIVYGTPY 298

[Close]

BLAST of Sgr019432 vs. ExPASy Swiss-Prot
Match: Q9SEL7 (Protease Do-like 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP5 PE=1 SV=3)

HSP 1 Score: 287.7 bits (735), Expect = 1.8e-76
Identity = 161/297 (54.21%), Postives = 205/297 (69.02%), Query Frame = 0


Query: 18  CSSENSMPFTS--RRAVVFASSALMASLLV------FPVPTYAALPQL---QDEVPQEED 77
           CS  N +      RR ++F SS  + S L+       P+ +  AL Q    ++E+ +EE+
Sbjct: 30  CSGSNHVDVIDRRRRIMIFGSSLALTSSLLGSNQQRLPMESAIALEQFKEKEEELEEEEE 89

Query: 78  RVVGLFQETSPSVVYIKDLEIAKKPQNSSEEAMFVEDENAKVKGTGSGFIWDKFGHIVTN 137
           R V LFQ+TSPSVVYI+ +E+ K    +S   +  ++EN K++GTGSGF+WDK GHIVTN
Sbjct: 90  RNVNLFQKTSPSVVYIEAIELPK----TSSGDILTDEENGKIEGTGSGFVWDKLGHIVTN 149

Query: 138 YHVVSTLATDISGLQRCKVNLVDAKGNVIYREAKIVGFDPEYDLAVLKVDLEGRELKPIV 197
           YHV++ LATD  GLQRCKV+LVDAKG    +E KIVG DP+ DLAVLK++ EGREL P+V
Sbjct: 150 YHVIAKLATDQFGLQRCKVSLVDAKGTRFSKEGKIVGLDPDNDLAVLKIETEGRELNPVV 209

Query: 198 LGTSQIYVLVRAAMPLAT--------LLQVISGLGREIPSPNGRAIRGAIQTDAAISAGN 257
           LGTS    + ++   +           + V+SGLGREIPSPNG++I  AIQTDA I++GN
Sbjct: 210 LGTSNDLRVGQSCFAIGNPYGYENTLTIGVVSGLGREIPSPNGKSISEAIQTDADINSGN 269

Query: 258 SGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVQR---QLFLNGTIYMTR 293
           SGGPL+DSYGH IGVNTATFTRKG+GMSSGVNFAIPIDTV R    L + GT Y  R
Sbjct: 270 SGGPLLDSYGHTIGVNTATFTRKGSGMSSGVNFAIPIDTVVRTVPYLIVYGTAYRDR 322

[Close]

BLAST of Sgr019432 vs. ExPASy Swiss-Prot
Match: O22609 (Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 SV=2)

HSP 1 Score: 149.8 bits (377), Expect = 5.9e-35
Identity = 105/255 (41.18%), Postives = 142/255 (55.69%), Query Frame = 0


Query: 33  VFASSALMASLLVFPVPTYAALPQLQDEVPQEEDRVVGLFQETSPSVVYIKDLEIAKKPQ 92
           +FA+S  + S   F V T         ++  +E   V LFQE +PSVVYI +L +     
Sbjct: 93  LFAASPAVESASAFVVST-------PKKLQTDELATVRLFQENTPSVVYITNLAV----- 152

Query: 93  NSSEEAMFVEDENAKVKGTGSGFIWDKFGHIVTNYHVVSTLATDISGLQRCKVNLVDAKG 152
               +  F  D     +G+GSGF+WDK GHIVTNYHV       I G    +V L D   
Sbjct: 153 ---RQDAFTLDVLEVPQGSGSGFVWDKQGHIVTNYHV-------IRGASDLRVTLADQ-- 212

Query: 153 NVIYREAKIVGFDPEYDLAVLKVDLEGRELKPIVLGTSQIYVLVRAAMPLAT-------- 212
                +AK+VGFD + D+AVL++D    +L+PI +G S   ++ +    +          
Sbjct: 213 --TTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLLVGQKVFAIGNPFGLDHTL 272

Query: 213 LLQVISGLGREIPS-PNGRAIRGAIQTDAAISAGNSGGPLVDSYGHVIGVNTATFTRKGT 272
              VISGL REI S   GR I+  IQTDAAI+ GNSGGPL+DS G +IG+NTA ++   +
Sbjct: 273 TTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGTLIGINTAIYS--PS 319

Query: 273 GMSSGVNFAIPIDTV 279
           G SSGV F+IP+DTV
Sbjct: 333 GASSGVGFSIPVDTV 319

[Close]

BLAST of Sgr019432 vs. ExPASy Swiss-Prot
Match: Q9LU10 (Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.0e-31
Identity = 96/228 (42.11%), Postives = 130/228 (57.02%), Query Frame = 0


Query: 65  EDRVVGLFQETSPSVVYIKDLEIAKKPQNSSEEAMFVEDENAKVKGTGSGFIWDKFGHIV 124
           E R+V LF++ + SVV I D+ +  +PQ      + + +      G GSG +WD  G+IV
Sbjct: 116 EGRIVQLFEKNTYSVVNIFDVTL--RPQLKMTGVVEIPE------GNGSGVVWDGQGYIV 175

Query: 125 TNYHVVSTLAT------DISGLQRCKVNLVDAKGNVIYREAKIVGFDPEYDLAVLKVDLE 184
           TNYHV+    +      D+ G    +VN++ + G     E K+VG D   DLAVLKVD  
Sbjct: 176 TNYHVIGNALSRNPSPGDVVG----RVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAP 235

Query: 185 GRELKPIVLGTSQIYVLVRAAMPLAT--------LLQVISGLGREIPSPNGRAIRGAIQT 244
              LKPI +G S    + +  + +           + VISGL R+I S  G  I G IQT
Sbjct: 236 ETLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQT 295

Query: 245 DAAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV 279
           DAAI+ GNSGGPL+DS G++IG+NTA FT+  TG S+GV FAIP  TV
Sbjct: 296 DAAINPGNSGGPLLDSKGNLIGINTAIFTQ--TGTSAGVGFAIPSSTV 329

[Close]

BLAST of Sgr019432 vs. ExPASy Swiss-Prot
Match: Q2SL36 (Probable periplasmic serine endoprotease DegP-like OS=Hahella chejuensis (strain KCTC 2396) OX=349521 GN=mucD PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 5.9e-19
Identity = 91/271 (33.58%), Postives = 131/271 (48.34%), Query Frame = 0


Query: 70  GLFQETSPSVVYIKDLEIAKKPQNSSEEAMFVEDENAKV--------------------K 129
           GL + TSP+VV   ++   +K  + S +  F   E  ++                    +
Sbjct: 33  GLVERTSPAVV---NISTVRKVGDDSAQYYFGGPEQDQIPEFFRHFFGDPYRRRGPQEAQ 92

Query: 130 GTGSGFIWDKFGHIVTNYHVVSTLATDISGLQRCKVNLVDAKGNVIYREAKIVGFDPEYD 189
            TGSGFI  K G+I+TN HVV       +G     V L+D +       AK++G D + D
Sbjct: 93  STGSGFIVSKDGYILTNNHVV-------AGADEIFVRLMDRR----ELTAKLIGSDEKSD 152

Query: 190 LAVLKVDLEGRELKPIVLG-TSQIYV---LVRAAMPLATLLQVISGL----GREIPSPNG 249
           LAVLKV  E  +L  + LG +S++ V   +V    P      V +G+    GR +P+ N 
Sbjct: 153 LAVLKV--EADDLPVLNLGKSSELKVGEWVVAIGSPFGFEYTVTAGIVSAKGRSLPNENY 212

Query: 250 RAIRGAIQTDAAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDT---V 309
                 IQTD AI+ GNSGGPL +  G V+G+N+  +TR G  M  GV+FAIPID    V
Sbjct: 213 VPF---IQTDVAINPGNSGGPLFNLEGEVVGINSQIYTRSGGFM--GVSFAIPIDVALDV 272

[Close]

BLAST of Sgr019432 vs. ExPASy Swiss-Prot
Match: A5W8F5 (Probable periplasmic serine endoprotease DegP-like OS=Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1) OX=351746 GN=Pput_4291 PE=3 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.9e-18
Identity = 74/214 (34.58%), Postives = 106/214 (49.53%), Query Frame = 0


Query: 103 DENAKVKGTGSGFIWDKFGHIVTNYHVVSTLATDISGLQRCKVNLVDAKGNVIYREAKIV 162
           D   + +  GSGFI    G+++TN HVV+     I       V L D        +AK+V
Sbjct: 92  DRQREAQSLGSGFIISSDGYVLTNNHVVADADEII-------VRLSDRS----ELQAKLV 151

Query: 163 GFDPEYDLAVLKVDLEGRELKPIVLGTSQIYVLVRAAMPLATLLQVISGLGREIPSPNGR 222
           G DP  D+A+LKVD  G+ L  + LG S+   +    + + +       + + I S  GR
Sbjct: 152 GTDPRTDVALLKVD--GKNLPTVKLGDSEKLKVGEWVLAIGSPFGFDHSVTKGIVSAKGR 211

Query: 223 AIRG-----AIQTDAAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDT 282
            +        IQTD AI+ GNSGGPL +  G V+G+N+  FTR G  M  G++FAIPID 
Sbjct: 212 TLPNDTYVPFIQTDVAINPGNSGGPLFNMKGEVVGINSQIFTRSGGFM--GLSFAIPIDV 271

Query: 283 ---VQRQLFLNGTIYMTRCTARVRDKNKTEAPLF 309
              V  QL  +G +        +++ NK  A  F
Sbjct: 272 AIDVSNQLKKDGKVSRGWLGVVIQEVNKDLAESF 290

[Close]

BLAST of Sgr019432 vs. ExPASy TrEMBL
Match: A0A6J1EKF8 (protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434145 PE=3 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 2.8e-120
Identity = 239/304 (78.62%), Postives = 258/304 (84.87%), Query Frame = 0


Query: 1   MALGSLGIRLLPIAAPLCSSENSMPFTSRRAVVFASSALMASLLVFPVPTYAALPQLQDE 60
           MALGSLGIRLLP+++P  SSE S+PFTSRRA+VFA +ALMASLL FPVP++AALPQLQDE
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFTSRRAIVFAPTALMASLLAFPVPSFAALPQLQDE 60

Query: 61  VPQEEDRVVGLFQETSPSVVYIKDLEIAKKPQNSSEEAMFVE-DENAKVKGTGSGFIWDK 120
           VPQEEDR+VGLFQETSPSVVYIK+LEIAKKPQN SEEAM +E DENAKVKGTGSGFIWDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVYIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 121 FGHIVTNYHVVSTLATDISGLQRCKVNLVDAKGNVIYREAKIVGFDPEYDLAVLKVDLEG 180
           FGHIVTNYHVVS LATD SGLQRCKVNLVD KGN I R+AKIVGFDPEYDLAVLKV+LEG
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELEG 180

Query: 181 RELKPIVLGTSQIYVLVRAAMPLAT--------LLQVISGLGREIPSPNGRAIRGAIQTD 240
            ELKPIV GTS+   + ++   +             VISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVQR---QLFLNGTI 293
           AAISAGNSGGPLVD YGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV R    L + GT 
Sbjct: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

[Close]

BLAST of Sgr019432 vs. ExPASy TrEMBL
Match: A0A6J1CEE6 (protease Do-like 5, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010776 PE=3 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 4.0e-119
Identity = 235/303 (77.56%), Postives = 254/303 (83.83%), Query Frame = 0


Query: 1   MALGSLGIRLLPIAAPLCSSENSMPFTSRRAVVFASSALMASLLVFPVPTYAALPQLQDE 60
           MAL SLGI LLPI AP  SS NS+PFTSRRA+VFA SALMASLL FP+PT+AALPQ+Q +
Sbjct: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQIQAQ 60

Query: 61  VPQEEDRVVGLFQETSPSVVYIKDLEIAKKPQNSSEEAMFVEDENAKVKGTGSGFIWDKF 120
           VPQEEDRVV LFQ+ SPSVVYIKDLE+AKKPQNSSEEA+ VEDEN KVKGTGSGF+WDKF
Sbjct: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF 120

Query: 121 GHIVTNYHVVSTLATDISGLQRCKVNLVDAKGNVIYREAKIVGFDPEYDLAVLKVDLEGR 180
           GHIVTNYHVVS LATD SGLQRCKVNLVDAKGN IYREAKIVGFDPEYDLAVLKV+L G 
Sbjct: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGC 180

Query: 181 ELKPIVLGTSQIYVLVRAAMPLAT--------LLQVISGLGREIPSPNGRAIRGAIQTDA 240
           ELKPIVLGTS+   + ++   +             VISGLGREIPSPNGRAIRG IQTDA
Sbjct: 181 ELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGGIQTDA 240

Query: 241 AISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVQR---QLFLNGTIY 293
           AIS+GNSGGPL+DSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV R    L + GT Y
Sbjct: 241 AISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPY 300

[Close]

BLAST of Sgr019432 vs. ExPASy TrEMBL
Match: A0A6J1CDW0 (protease Do-like 5, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010776 PE=3 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 6.8e-119
Identity = 231/292 (79.11%), Postives = 250/292 (85.62%), Query Frame = 0


Query: 1   MALGSLGIRLLPIAAPLCSSENSMPFTSRRAVVFASSALMASLLVFPVPTYAALPQLQDE 60
           MAL SLGI LLPI AP  SS NS+PFTSRRA+VFA SALMASLL FP+PT+AALPQ+Q +
Sbjct: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQIQAQ 60

Query: 61  VPQEEDRVVGLFQETSPSVVYIKDLEIAKKPQNSSEEAMFVEDENAKVKGTGSGFIWDKF 120
           VPQEEDRVV LFQ+ SPSVVYIKDLE+AKKPQNSSEEA+ VEDEN KVKGTGSGF+WDKF
Sbjct: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF 120

Query: 121 GHIVTNYHVVSTLATDISGLQRCKVNLVDAKGNVIYREAKIVGFDPEYDLAVLKVDLEGR 180
           GHIVTNYHVVS LATD SGLQRCKVNLVDAKGN IYREAKIVGFDPEYDLAVLKV+L G 
Sbjct: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGC 180

Query: 181 ELKPIVLGTSQIYVLVRAAMPLAT--------LLQVISGLGREIPSPNGRAIRGAIQTDA 240
           ELKPIVLGTS+   + ++   +             VISGLGREIPSPNGRAIRG IQTDA
Sbjct: 181 ELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGGIQTDA 240

Query: 241 AISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVQRQLFL 285
           AIS+GNSGGPL+DSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV  Q F+
Sbjct: 241 AISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVGTQPFM 292

[Close]

BLAST of Sgr019432 vs. ExPASy TrEMBL
Match: A0A6J1KMS0 (protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495580 PE=3 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 8.3e-117
Identity = 234/304 (76.97%), Postives = 255/304 (83.88%), Query Frame = 0


Query: 1   MALGSLGIRLLPIAAPLCSSENSMPFTSRRAVVFASSALMASLLVFPVPTYAALPQLQDE 60
           MALGSLGIRLLP+++P  SSE S+PF+SRRA+VFA + LMASLL FPVP++AAL QLQDE
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE 60

Query: 61  VPQEEDRVVGLFQETSPSVVYIKDLEIAKKPQNSSEEAMFVE-DENAKVKGTGSGFIWDK 120
           VPQEEDR+VGLFQETSPSVV IK+LEIAKKPQN SEEAM +E DENAKVKGTGSGFIWDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 121 FGHIVTNYHVVSTLATDISGLQRCKVNLVDAKGNVIYREAKIVGFDPEYDLAVLKVDLEG 180
           FGHIVTNYHVVS LATD SGLQRCKVNLVD KGN I R+AKIVGFDPEYDLAVLKV+L+G
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQG 180

Query: 181 RELKPIVLGTSQIYVLVRAAMPLAT--------LLQVISGLGREIPSPNGRAIRGAIQTD 240
            ELKPIV GTS+   + ++   +             VISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVQR---QLFLNGTI 293
           AAISAGNSGGPLVD YGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV R    L + GT 
Sbjct: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

[Close]

BLAST of Sgr019432 vs. ExPASy TrEMBL
Match: A0A5D3DAW0 (Protease Do-like 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G00660 PE=3 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 4.3e-113
Identity = 227/304 (74.67%), Postives = 247/304 (81.25%), Query Frame = 0


Query: 1   MALGSLGIRLLPIAAPLCSSENSMPFTSRRAVVFASSALMASLLVFPVPTYAALPQLQDE 60
           MALG LGI  LPI AP  SSEN +PFTSRRA++F+ +ALMASLL FP+PT AALPQLQD 
Sbjct: 1   MALGLLGIPPLPIPAPPNSSENPLPFTSRRAILFSPTALMASLLAFPLPTPAALPQLQDP 60

Query: 61  VPQEEDRVVGLFQETSPSVVYIKDLEIAKKPQNSSEE-AMFVEDENAKVKGTGSGFIWDK 120
           + QEEDR+V LFQETSPSVVYIKDLE+AK PQN SEE  M +ED+N KVKGTGSGF+WDK
Sbjct: 61  LLQEEDRIVSLFQETSPSVVYIKDLELAKNPQNRSEEPPMLIEDDNVKVKGTGSGFVWDK 120

Query: 121 FGHIVTNYHVVSTLATDISGLQRCKVNLVDAKGNVIYREAKIVGFDPEYDLAVLKVDLEG 180
           FGHIVTNYHVVS LATD SG QRCKVNLVD KGN IY+EA IVGFDPEYDLAVLKV+LEG
Sbjct: 121 FGHIVTNYHVVSALATDNSGSQRCKVNLVDVKGNGIYKEANIVGFDPEYDLAVLKVELEG 180

Query: 181 RELKPIVLGTSQIYVLVRAAMPLAT--------LLQVISGLGREIPSPNGRAIRGAIQTD 240
            ELKPIV GTS+   + ++   +             VISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVQR---QLFLNGTI 293
           AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV R    L + GT 
Sbjct: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

[Close]

BLAST of Sgr019432 vs. TAIR 10
Match: AT4G18370.1 (DEGP protease 5 )

HSP 1 Score: 287.7 bits (735), Expect = 1.3e-77
Identity = 161/297 (54.21%), Postives = 205/297 (69.02%), Query Frame = 0


Query: 18  CSSENSMPFTS--RRAVVFASSALMASLLV------FPVPTYAALPQL---QDEVPQEED 77
           CS  N +      RR ++F SS  + S L+       P+ +  AL Q    ++E+ +EE+
Sbjct: 30  CSGSNHVDVIDRRRRIMIFGSSLALTSSLLGSNQQRLPMESAIALEQFKEKEEELEEEEE 89

Query: 78  RVVGLFQETSPSVVYIKDLEIAKKPQNSSEEAMFVEDENAKVKGTGSGFIWDKFGHIVTN 137
           R V LFQ+TSPSVVYI+ +E+ K    +S   +  ++EN K++GTGSGF+WDK GHIVTN
Sbjct: 90  RNVNLFQKTSPSVVYIEAIELPK----TSSGDILTDEENGKIEGTGSGFVWDKLGHIVTN 149

Query: 138 YHVVSTLATDISGLQRCKVNLVDAKGNVIYREAKIVGFDPEYDLAVLKVDLEGRELKPIV 197
           YHV++ LATD  GLQRCKV+LVDAKG    +E KIVG DP+ DLAVLK++ EGREL P+V
Sbjct: 150 YHVIAKLATDQFGLQRCKVSLVDAKGTRFSKEGKIVGLDPDNDLAVLKIETEGRELNPVV 209

Query: 198 LGTSQIYVLVRAAMPLAT--------LLQVISGLGREIPSPNGRAIRGAIQTDAAISAGN 257
           LGTS    + ++   +           + V+SGLGREIPSPNG++I  AIQTDA I++GN
Sbjct: 210 LGTSNDLRVGQSCFAIGNPYGYENTLTIGVVSGLGREIPSPNGKSISEAIQTDADINSGN 269

Query: 258 SGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVQR---QLFLNGTIYMTR 293
           SGGPL+DSYGH IGVNTATFTRKG+GMSSGVNFAIPIDTV R    L + GT Y  R
Sbjct: 270 SGGPLLDSYGHTIGVNTATFTRKGSGMSSGVNFAIPIDTVVRTVPYLIVYGTAYRDR 322

[Close]

BLAST of Sgr019432 vs. TAIR 10
Match: AT3G27925.1 (DegP protease 1 )

HSP 1 Score: 149.8 bits (377), Expect = 4.2e-36
Identity = 105/255 (41.18%), Postives = 142/255 (55.69%), Query Frame = 0


Query: 33  VFASSALMASLLVFPVPTYAALPQLQDEVPQEEDRVVGLFQETSPSVVYIKDLEIAKKPQ 92
           +FA+S  + S   F V T         ++  +E   V LFQE +PSVVYI +L +     
Sbjct: 93  LFAASPAVESASAFVVST-------PKKLQTDELATVRLFQENTPSVVYITNLAV----- 152

Query: 93  NSSEEAMFVEDENAKVKGTGSGFIWDKFGHIVTNYHVVSTLATDISGLQRCKVNLVDAKG 152
               +  F  D     +G+GSGF+WDK GHIVTNYHV       I G    +V L D   
Sbjct: 153 ---RQDAFTLDVLEVPQGSGSGFVWDKQGHIVTNYHV-------IRGASDLRVTLADQ-- 212

Query: 153 NVIYREAKIVGFDPEYDLAVLKVDLEGRELKPIVLGTSQIYVLVRAAMPLAT-------- 212
                +AK+VGFD + D+AVL++D    +L+PI +G S   ++ +    +          
Sbjct: 213 --TTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLLVGQKVFAIGNPFGLDHTL 272

Query: 213 LLQVISGLGREIPS-PNGRAIRGAIQTDAAISAGNSGGPLVDSYGHVIGVNTATFTRKGT 272
              VISGL REI S   GR I+  IQTDAAI+ GNSGGPL+DS G +IG+NTA ++   +
Sbjct: 273 TTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGTLIGINTAIYS--PS 319

Query: 273 GMSSGVNFAIPIDTV 279
           G SSGV F+IP+DTV
Sbjct: 333 GASSGVGFSIPVDTV 319

[Close]

BLAST of Sgr019432 vs. TAIR 10
Match: AT5G39830.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 139.0 bits (349), Expect = 7.3e-33
Identity = 96/228 (42.11%), Postives = 130/228 (57.02%), Query Frame = 0


Query: 65  EDRVVGLFQETSPSVVYIKDLEIAKKPQNSSEEAMFVEDENAKVKGTGSGFIWDKFGHIV 124
           E R+V LF++ + SVV I D+ +  +PQ      + + +      G GSG +WD  G+IV
Sbjct: 116 EGRIVQLFEKNTYSVVNIFDVTL--RPQLKMTGVVEIPE------GNGSGVVWDGQGYIV 175

Query: 125 TNYHVVSTLAT------DISGLQRCKVNLVDAKGNVIYREAKIVGFDPEYDLAVLKVDLE 184
           TNYHV+    +      D+ G    +VN++ + G     E K+VG D   DLAVLKVD  
Sbjct: 176 TNYHVIGNALSRNPSPGDVVG----RVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAP 235

Query: 185 GRELKPIVLGTSQIYVLVRAAMPLAT--------LLQVISGLGREIPSPNGRAIRGAIQT 244
              LKPI +G S    + +  + +           + VISGL R+I S  G  I G IQT
Sbjct: 236 ETLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQT 295

Query: 245 DAAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV 279
           DAAI+ GNSGGPL+DS G++IG+NTA FT+  TG S+GV FAIP  TV
Sbjct: 296 DAAINPGNSGGPLLDSKGNLIGINTAIFTQ--TGTSAGVGFAIPSSTV 329

[Close]

BLAST of Sgr019432 vs. TAIR 10
Match: AT5G39830.2 (Trypsin family protein with PDZ domain )

HSP 1 Score: 128.6 bits (322), Expect = 9.9e-30
Identity = 106/311 (34.08%), Postives = 156/311 (50.16%), Query Frame = 0


Query: 65  EDRVVGLFQETSPSVVYIKDLEIAKKPQNSSEEAMFVEDENAKVKGTGSGFIWDKFGHIV 124
           E R+V LF++ + SVV I D+ +  +PQ      + + +      G GSG +WD  G+IV
Sbjct: 116 EGRIVQLFEKNTYSVVNIFDVTL--RPQLKMTGVVEIPE------GNGSGVVWDGQGYIV 175

Query: 125 TNYHVVSTLAT------DISGLQRCKVNLVDAKGNVIYREAKIVGFDPEYDLAVLKVDLE 184
           TNYHV+    +      D+ G    +VN++ + G     E K+VG D   DLAVLKVD  
Sbjct: 176 TNYHVIGNALSRNPSPGDVVG----RVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAP 235

Query: 185 GRELKPIVLGTSQIYVLVRAAMPLAT--------LLQVISGLGREIPSPNGRAIRGAIQT 244
              LKPI +G S    + +  + +           + VISGL R+I S  G  I G IQT
Sbjct: 236 ETLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQT 295

Query: 245 DAAISAGNSGGPLVDSYGHVIGVNTATFTRK-----------GTGMSSGVNFAIPIDTVQ 304
           DAAI+ GNSGGPL+DS G++IG+NTA FT+               + +G+N  +  D V 
Sbjct: 296 DAAINPGNSGGPLLDSKGNLIGINTAIFTQTVLKIVPQLIQFSKVLRAGINIELAPDPVA 355

Query: 305 RQLFL-NGTIYMTRCTARVRDKNKTEAPLFHWRWRATSSATILYCILKNDISLLVNAVKR 350
            QL + NG + +     +V  K+  E    H   R  +   +L  I+   +++    VK 
Sbjct: 356 NQLNVRNGALVL-----QVPGKSLAEKAGLHPTSRGFAGNIVLGDII---VAVDDKPVKN 406

[Close]

BLAST of Sgr019432 vs. TAIR 10
Match: AT5G27660.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 58.2 bits (139), Expect = 1.6e-08
Identity = 56/181 (30.94%), Postives = 86/181 (47.51%), Query Frame = 0


Query: 109 KGTGSGFIWDKFGHIVTNYHVVSTLATDISGLQRCKVNLVDAKGNVIYREAKIVGFDPEY 168
           K  GSG I D  G I+T  HVV     +I    + +V++    G     E  +V  D + 
Sbjct: 146 KSIGSGTIIDADGTILTCAHVVVDF-QNIRHSSKGRVDVTLQDGRTF--EGVVVNADLQS 205

Query: 169 DLAVLKVDLEGRELKPIVLGTSQIY----VLVRAAMPLATLLQVISGLGREIPSPN---- 228
           D+A++K+  +   L    LG S        ++    PL+    V +G+   +   +    
Sbjct: 206 DIALVKIKSK-TPLPTAKLGFSSKLRPGDWVIAVGCPLSLQNTVTAGIVSCVDRKSSDLG 265

Query: 229 -GRAIRGAIQTDAAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVQ 281
            G   R  +QTD +I+AGNSGGPLV+  G VIGVN           + G+ F++PID+V 
Sbjct: 266 LGGKHREYLQTDCSINAGNSGGPLVNLDGEVIGVNIMKVL-----AADGLGFSVPIDSVS 317

The following BLAST results are available for this feature:

BLAST of Sgr019432 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022927238.1	5.7e-120	78.62	protease Do-like 5, chloroplastic isoform X1 [Cucurbita moschata]	[more]
XP_022140016.1	8.2e-119	77.56	protease Do-like 5, chloroplastic isoform X2 [Momordica charantia]	[more]
XP_022140015.1	1.4e-118	79.11	protease Do-like 5, chloroplastic isoform X1 [Momordica charantia]	[more]
KAG6583889.1	3.1e-118	79.93	Protease Do-like 5, chloroplastic, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038893976.1	3.4e-117	76.90	protease Do-like 5, chloroplastic [Benincasa hispida]	[more]

BLAST of Sgr019432 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SEL7	1.8e-76	54.21	Protease Do-like 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP5 PE=1 ...	[more]
O22609	5.9e-35	41.18	Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 ...	[more]
Q9LU10	1.0e-31	42.11	Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 ...	[more]
Q2SL36	5.9e-19	33.58	Probable periplasmic serine endoprotease DegP-like OS=Hahella chejuensis (strain...	[more]
A5W8F5	2.9e-18	34.58	Probable periplasmic serine endoprotease DegP-like OS=Pseudomonas putida (strain...	[more]

BLAST of Sgr019432 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EKF8	2.8e-120	78.62	protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1CEE6	4.0e-119	77.56	protease Do-like 5, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1CDW0	6.8e-119	79.11	protease Do-like 5, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1KMS0	8.3e-117	76.97	protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A5D3DAW0	4.3e-113	74.67	Protease Do-like 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G0...	[more]

BLAST of Sgr019432 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G18370.1	1.3e-77	54.21	DEGP protease 5	[more]
AT3G27925.1	4.2e-36	41.18	DegP protease 1	[more]
AT5G39830.1	7.3e-33	42.11	Trypsin family protein with PDZ domain	[more]
AT5G39830.2	9.9e-30	34.08	Trypsin family protein with PDZ domain	[more]
AT5G27660.1	1.6e-08	30.94	Trypsin family protein with PDZ domain	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001940	Peptidase S1C	PRINTS	PR00834	PROTEASES2C	coord: 121..133 score: 50.47 coord: 224..241 score: 62.74 coord: 246..263 score: 37.67
IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	GENE3D	2.40.10.10		coord: 196..287 e-value: 1.4E-25 score: 91.7
IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	GENE3D	2.40.10.10		coord: 59..185 e-value: 1.1E-31 score: 111.0
None	No IPR available	PFAM	PF13365	Trypsin_2	coord: 112..253 e-value: 4.5E-21 score: 76.4
None	No IPR available	PANTHER	PTHR43343	PEPTIDASE S12	coord: 19..289
None	No IPR available	PANTHER	PTHR43343:SF6	PROTEASE DO-LIKE 5, CHLOROPLASTIC ISOFORM X1	coord: 19..289
IPR009003	Peptidase S1, PA clan	SUPERFAMILY	50494	Trypsin-like serine proteases	coord: 70..280

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr019432.1	Sgr019432.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0004252	serine-type endopeptidase activity