Sgr018086 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr018086 vs. NCBI nr
Match: XP_022155823.1 (nuclear intron maturase 3, mitochondrial [Momordica charantia] >XP_022155825.1 nuclear intron maturase 3, mitochondrial [Momordica charantia])

HSP 1 Score: 1321.6 bits (3419), Expect = 0.0e+00
Identity = 673/756 (89.02%), Postives = 706/756 (93.39%), Query Frame = 0

Query: 1   MLSTSFNKLYSDLPLNPS-PLLKFPFQHSAETLTSSQLKALVLSRFTQGKFFDLLQNVVA 60
           +LSTSFNKLYSDLPL  S PL  FPFQHS ETLT  +LKALVLSRFT GKF DLLQNVVA
Sbjct: 15  ILSTSFNKLYSDLPLKHSPPLHNFPFQHSTETLTWPELKALVLSRFTHGKFLDLLQNVVA 74

Query: 61  SPSVLLTASQNLVT-PPPSNGLNAPDSLPIVDLVSKCFSVEEMARELYENRFDVGACCVR 120
           SPSVLLTASQNL+T PPPSNGLNAPD LPI+DLVSKCFSVEEMARELYE+RFDVGACCVR
Sbjct: 75  SPSVLLTASQNLITPPPPSNGLNAPDPLPILDLVSKCFSVEEMARELYEDRFDVGACCVR 134

Query: 121 MVPSDEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKHS 180
           M   D+KG+SLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLK+S
Sbjct: 135 M---DQKGQSLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNS 194

Query: 181 VQNPSWWFTIAFHRNKFDSVHVNKLCLLVEEKIKDDILIYMLKKLFELEAIQIELGACYL 240
           VQNPSWWFT+AF R KFDSVHV+KLCLLVEEKIKD +LI MLKKLFELEAIQIELGACYL
Sbjct: 195 VQNPSWWFTVAFRRKKFDSVHVHKLCLLVEEKIKDHLLICMLKKLFELEAIQIELGACYL 254

Query: 241 GRGFPQESGLCSILFNIYFNGFDKEIQQIRLQKNKENPKFNPVEMVSFHNPVKIYAVRYL 300
           GRGFPQESGLCSIL NI+FNGFDK+IQQIRLQKN+ENPKF+  E+VSFH+PVKIYAVRYL
Sbjct: 255 GRGFPQESGLCSILCNIFFNGFDKDIQQIRLQKNEENPKFSLDEIVSFHSPVKIYAVRYL 314

Query: 301 DEILVITSGSKMLTMDLKGQVLKYLERNLELEVDRMNSAIHSAVSEKIRFLGMELHAVPP 360
           DEILVITSGSKMLTMDLK QVLKYLE NLELEVDRMN+AIHSAVSEKI FLGMEL AVPP
Sbjct: 315 DEILVITSGSKMLTMDLKSQVLKYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPP 374

Query: 361 SVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTNGFKF 420
           SVLHPPMSEKAIRARKKY+RQKEVR IELRNARERNRKKLGLKILSHVFKKLKQT+GFKF
Sbjct: 375 SVLHPPMSEKAIRARKKYIRQKEVRVIELRNARERNRKKLGLKILSHVFKKLKQTDGFKF 434

Query: 421 KFQIEEEVREIFSNWADEVVQDFFEYLEDYAEWHRVLLRGDFLHLKHIRNQLPEDLVNAY 480
           +FQIE+EVREIF NWADEV Q FFE LE++AEWH  L  GDFL LKHIRNQLPEDLVNAY
Sbjct: 435 EFQIEKEVREIFRNWADEVAQHFFESLENHAEWHHALSAGDFLSLKHIRNQLPEDLVNAY 494

Query: 481 DRFQDQVDKHLNPVKVKNEKAREDEEKRVEEEQQYAKRTVEDLRRLCIKVDAPIELVRNA 540
           DRFQDQVDKHLNPVKVK  KAREDEEKRVEEEQ+YA+RTVEDL RLCIKVDAPIELVR A
Sbjct: 495 DRFQDQVDKHLNPVKVKYVKAREDEEKRVEEEQKYARRTVEDLTRLCIKVDAPIELVRKA 554

Query: 541 VKMVGFTNKMGRPQPISSLVALEDSDIIKWYAGVGRRWLDFFCCCDNYKMVKTVVTYHLR 600
           VKMVGFTNKMGRPQPIS LVALED DIIKWYAGVGRRWLDFFCCC NYKMVKTVVTYHLR
Sbjct: 555 VKMVGFTNKMGRPQPISLLVALEDIDIIKWYAGVGRRWLDFFCCCHNYKMVKTVVTYHLR 614

Query: 601 FSCILTLAEKHESTKREAMKHYSKDLKVFDLKGNEEMHFPTEREVKMLGDRNLADPYPVD 660
           FSCILTLAEKHESTKREAMKH+SKDLKVFDL G+EE+HFPTEREVKMLGDR LADPYPVD
Sbjct: 615 FSCILTLAEKHESTKREAMKHFSKDLKVFDLNGDEEIHFPTEREVKMLGDRMLADPYPVD 674

Query: 661 GALSLFLIRLATDEPSYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGEKWIRGMGVMHE 720
           G LSLFLIRLA DEPS PCIAHFCNRTDSILYRVRLLQRTLNVN  +GEKW+RGMGV+HE
Sbjct: 675 GTLSLFLIRLAIDEPSCPCIAHFCNRTDSILYRVRLLQRTLNVNSCDGEKWVRGMGVIHE 734

Query: 721 SLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 755
           SLNQRCLPLCADHISDLYMG+INLQDLDCTLSLDMD
Sbjct: 735 SLNQRCLPLCADHISDLYMGRINLQDLDCTLSLDMD 767

BLAST of Sgr018086 vs. NCBI nr
Match: KAG6600779.1 (Nuclear intron maturase 3, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1313.5 bits (3398), Expect = 0.0e+00
Identity = 657/753 (87.25%), Postives = 700/753 (92.96%), Query Frame = 0

Query: 2   LSTSFNKLYSDLPLNPSPLLKFPFQHSAETLTSSQLKALVLSRFTQGKFFDLLQNVVASP 61
           LS+SFNKLYSD PL  SPLLK PFQHS +TLT  QL+ALVLSRF+QGKFFDLLQNVVASP
Sbjct: 16  LSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFSQGKFFDLLQNVVASP 75

Query: 62  SVLLTASQNLVTPPPSNGLNAPDSLPIVDLVSKCFSVEEMARELYENRFDVGACCVRMVP 121
           SVL TASQNL+TP PSN LNAP+SL  +D+VS CFSVE+MARELYENRFDVGACCVR+ P
Sbjct: 76  SVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELYENRFDVGACCVRLEP 135

Query: 122 SDEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKHSVQN 181
           S+EKGE LVLPNLKLKVL+EAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLK+SVQN
Sbjct: 136 SEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQN 195

Query: 182 PSWWFTIAFHRNKFDSVHVNKLCLLVEEKIKDDILIYMLKKLFELEAIQIELGACYLGRG 241
           PSWWFT+AF R KFDSVHVNKLCLLV+EKIKDDILI MLKKLFELEA+QIELG CYLGRG
Sbjct: 196 PSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFELEAVQIELGGCYLGRG 255

Query: 242 FPQESGLCSILFNIYFNGFDKEIQQIRLQKNKENPKFNPVEMVSFHNPVKIYAVRYLDEI 301
           FPQESGLCSIL NIYFNGFDKEIQQIRLQKN+ENPKF+    VSFHNPVKIYAVRYLDEI
Sbjct: 256 FPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSMDGTVSFHNPVKIYAVRYLDEI 315

Query: 302 LVITSGSKMLTMDLKGQVLKYLERNLELEVDRMNSAIHSAVSEKIRFLGMELHAVPPSVL 361
           LVITSGSKML M+LK QVL+YLE NLELEVDRMN+AIHSAVSEKI FLGMEL AVPPSVL
Sbjct: 316 LVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVL 375

Query: 362 HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTNGFKFKFQ 421
           HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLK+T+G K +FQ
Sbjct: 376 HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKRTDGLKSEFQ 435

Query: 422 IEEEVREIFSNWADEVVQDFFEYLEDYAEWHRVLLRGDFLHLKHIRNQLPEDLVNAYDRF 481
           IE+EV EIF NWADEVV+DFFE LED  EWHR L  GDFL LKHIRNQLP DLVNAYDRF
Sbjct: 436 IEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHIRNQLPVDLVNAYDRF 495

Query: 482 QDQVDKHLNPVKVKNEKAREDEEKRVEEEQQYAKRTVEDLRRLCIKVDAPIELVRNAVKM 541
           QDQV++HLNP+K K EKAREDEEKR+ EE++YAKRTVEDL RLCIKV+APIELVR AVKM
Sbjct: 496 QDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCIKVEAPIELVRKAVKM 555

Query: 542 VGFTNKMGRPQPISSLVALEDSDIIKWYAGVGRRWLDFFCCCDNYKMVKTVVTYHLRFSC 601
           +GFTNKMGRP+PISSL+ALED+DIIKWYAGVGRRWLDFFCCC NYKMVKT+VTYHLRFSC
Sbjct: 556 IGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTIVTYHLRFSC 615

Query: 602 ILTLAEKHESTKREAMKHYSKDLKVFDLKGNEEMHFPTEREVKMLGDRNLADPYPVDGAL 661
           ILTLAEKHESTKREAMKHYSKDLKVFDL G EEMHFPTEREVKMLG+RNLADPYPVDGA 
Sbjct: 616 ILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKMLGERNLADPYPVDGAF 675

Query: 662 SLFLIRLATDEPSYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGEKWIRGMGVMHESLN 721
           SLFLIRL TDE SYPCIAHFCNRTDSILYRVRLLQ+TLNVNPSNG +W+RGMGV+HESLN
Sbjct: 676 SLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNGVEWVRGMGVIHESLN 735

Query: 722 QRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 755
           QRCLPLCADHISDLYMGKINLQDLDCTLSLDMD
Sbjct: 736 QRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Sgr018086 vs. NCBI nr
Match: KAG7031416.1 (Nuclear intron maturase 3, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1312.0 bits (3394), Expect = 0.0e+00
Identity = 656/753 (87.12%), Postives = 700/753 (92.96%), Query Frame = 0

Query: 2   LSTSFNKLYSDLPLNPSPLLKFPFQHSAETLTSSQLKALVLSRFTQGKFFDLLQNVVASP 61
           LS+SFNKLYSD PL  SPLLK PFQHS +TLT  QL+ALVLSRF+QGKFFDLLQNVVASP
Sbjct: 16  LSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFSQGKFFDLLQNVVASP 75

Query: 62  SVLLTASQNLVTPPPSNGLNAPDSLPIVDLVSKCFSVEEMARELYENRFDVGACCVRMVP 121
           SVL TASQNL+TP PSN LNAP+SL  +D+VS CFSVE+MARELYENRFDVGACCVR+ P
Sbjct: 76  SVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELYENRFDVGACCVRLEP 135

Query: 122 SDEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKHSVQN 181
           S+EKGE LVLPNLKLKVL+EAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLK+SVQN
Sbjct: 136 SEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQN 195

Query: 182 PSWWFTIAFHRNKFDSVHVNKLCLLVEEKIKDDILIYMLKKLFELEAIQIELGACYLGRG 241
           PSWWFT+AF R KFDSVHVNKLCLLV+EKIKDDILI MLKKLFELEA+QIELG CYLGRG
Sbjct: 196 PSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFELEAVQIELGGCYLGRG 255

Query: 242 FPQESGLCSILFNIYFNGFDKEIQQIRLQKNKENPKFNPVEMVSFHNPVKIYAVRYLDEI 301
           FPQESGLCSIL NIYFNGFDKEIQQIRLQKN+ENPKF+    VSFHNPVKIYAVRYLDEI
Sbjct: 256 FPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVSFHNPVKIYAVRYLDEI 315

Query: 302 LVITSGSKMLTMDLKGQVLKYLERNLELEVDRMNSAIHSAVSEKIRFLGMELHAVPPSVL 361
           LVITSGSKML M+LK QVL+YLE NLELEVDRMN+AIHSAVSEKI FLGMEL AVPPSVL
Sbjct: 316 LVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVL 375

Query: 362 HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTNGFKFKFQ 421
           HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLK+T+G K +FQ
Sbjct: 376 HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKRTDGLKSEFQ 435

Query: 422 IEEEVREIFSNWADEVVQDFFEYLEDYAEWHRVLLRGDFLHLKHIRNQLPEDLVNAYDRF 481
           IE+EV EIF NWADEVV+DFFE LED  EWHR L  GDFL LKHIRNQLP DLVNAYDRF
Sbjct: 436 IEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHIRNQLPVDLVNAYDRF 495

Query: 482 QDQVDKHLNPVKVKNEKAREDEEKRVEEEQQYAKRTVEDLRRLCIKVDAPIELVRNAVKM 541
           QDQV++HLNP+K K EKAREDEEKR+ EE++YAKRTVEDL RLCIKV+APIELVR AVKM
Sbjct: 496 QDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCIKVEAPIELVRKAVKM 555

Query: 542 VGFTNKMGRPQPISSLVALEDSDIIKWYAGVGRRWLDFFCCCDNYKMVKTVVTYHLRFSC 601
           +GFTNKMGRP+PISSL+ALED+DIIKWYAGVGRRWLDFFCCC NYKMVKT+VTYHLRFSC
Sbjct: 556 IGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTIVTYHLRFSC 615

Query: 602 ILTLAEKHESTKREAMKHYSKDLKVFDLKGNEEMHFPTEREVKMLGDRNLADPYPVDGAL 661
           ILTLAEKHESTKREAMKHYSKDLKVFDL G EEMHFPTEREVKMLG+RNLADPYPVDGA 
Sbjct: 616 ILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKMLGERNLADPYPVDGAF 675

Query: 662 SLFLIRLATDEPSYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGEKWIRGMGVMHESLN 721
           SLFLIRL TDE SYPCIAHFCNRTDSILYRVRLLQ+TLNVNPSNG +W+RGMGV+HESLN
Sbjct: 676 SLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNGVEWVRGMGVIHESLN 735

Query: 722 QRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 755
           QRCLPLCADHI+DLYMGKINLQDLDCTLSLDMD
Sbjct: 736 QRCLPLCADHINDLYMGKINLQDLDCTLSLDMD 768

BLAST of Sgr018086 vs. NCBI nr
Match: XP_022976163.1 (nuclear intron maturase 3, mitochondrial [Cucurbita maxima])

HSP 1 Score: 1306.2 bits (3379), Expect = 0.0e+00
Identity = 654/753 (86.85%), Postives = 697/753 (92.56%), Query Frame = 0

Query: 2   LSTSFNKLYSDLPLNPSPLLKFPFQHSAETLTSSQLKALVLSRFTQGKFFDLLQNVVASP 61
           LS+S NKLYSD PL  SPLLK PFQHS +TLT  QL+ALVLSRF+QGKFFDLLQNVVASP
Sbjct: 16  LSSSLNKLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFSQGKFFDLLQNVVASP 75

Query: 62  SVLLTASQNLVTPPPSNGLNAPDSLPIVDLVSKCFSVEEMARELYENRFDVGACCVRMVP 121
           SVL TASQNL+TP PSN LNAP+SL  +D+VS CFSVE+MARELYENRFDVGACCVR+  
Sbjct: 76  SVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELYENRFDVGACCVRLES 135

Query: 122 SDEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKHSVQN 181
           S+EKGE LVLPNLKLKVL+EA++MVLEIVYDERFVTFSYGGRVGMGRHTAIRYLK+SVQN
Sbjct: 136 SEEKGEFLVLPNLKLKVLLEAMKMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQN 195

Query: 182 PSWWFTIAFHRNKFDSVHVNKLCLLVEEKIKDDILIYMLKKLFELEAIQIELGACYLGRG 241
           PSWWFT+AF R KFDSVHVNKLCLLV+EKIKDDILI MLKKLFELEA+QIELG CYLGRG
Sbjct: 196 PSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFELEAVQIELGGCYLGRG 255

Query: 242 FPQESGLCSILFNIYFNGFDKEIQQIRLQKNKENPKFNPVEMVSFHNPVKIYAVRYLDEI 301
           FPQESGLCSIL NIYFNGFDKEIQQIRLQKN+ENPKF+    VSFHNPVKIYAVRYLDEI
Sbjct: 256 FPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLNGTVSFHNPVKIYAVRYLDEI 315

Query: 302 LVITSGSKMLTMDLKGQVLKYLERNLELEVDRMNSAIHSAVSEKIRFLGMELHAVPPSVL 361
           LVITSGSKM  M+LK QVL+YLE NLELEVDRMN+AIHSAVSEKI FLGMEL AVPPSVL
Sbjct: 316 LVITSGSKMQIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVL 375

Query: 362 HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTNGFKFKFQ 421
           HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLK+T+G K +FQ
Sbjct: 376 HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKRTDGLKSEFQ 435

Query: 422 IEEEVREIFSNWADEVVQDFFEYLEDYAEWHRVLLRGDFLHLKHIRNQLPEDLVNAYDRF 481
           IE+EV EIF NWADEVV+DFFE LED  EWHR L  GDFL LKHIRNQLP DLVNAYDRF
Sbjct: 436 IEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHIRNQLPVDLVNAYDRF 495

Query: 482 QDQVDKHLNPVKVKNEKAREDEEKRVEEEQQYAKRTVEDLRRLCIKVDAPIELVRNAVKM 541
           QDQV+KHLNP+K K EKAREDEEKR+ EE++YAKRTVEDL RLCIKV+APIELVR AVKM
Sbjct: 496 QDQVNKHLNPLKAKKEKAREDEEKRLVEEERYAKRTVEDLTRLCIKVEAPIELVRKAVKM 555

Query: 542 VGFTNKMGRPQPISSLVALEDSDIIKWYAGVGRRWLDFFCCCDNYKMVKTVVTYHLRFSC 601
           +GFTNKMGRPQPISSL+ALED+DIIKWYAGVGRRWLDFFCCC NYKMVKT+VTYHLRFSC
Sbjct: 556 IGFTNKMGRPQPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTIVTYHLRFSC 615

Query: 602 ILTLAEKHESTKREAMKHYSKDLKVFDLKGNEEMHFPTEREVKMLGDRNLADPYPVDGAL 661
           ILTLAEKHESTKREAMKHYSKDLKVFDL G EEMHFPTEREVKMLG+RNLADPYPVDGA 
Sbjct: 616 ILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKMLGERNLADPYPVDGAF 675

Query: 662 SLFLIRLATDEPSYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGEKWIRGMGVMHESLN 721
           SLFLIRL TDE SYPCIAHFCNRTDSILYRVRLLQ+TLNVNPSNG +W+RGMGV+HESLN
Sbjct: 676 SLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNGVEWVRGMGVIHESLN 735

Query: 722 QRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 755
           QRCLPLCADHISDLYMGKINLQDLDCTLSLDMD
Sbjct: 736 QRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Sgr018086 vs. NCBI nr
Match: XP_022942578.1 (nuclear intron maturase 3, mitochondrial [Cucurbita moschata])

HSP 1 Score: 1302.7 bits (3370), Expect = 0.0e+00
Identity = 653/753 (86.72%), Postives = 697/753 (92.56%), Query Frame = 0

Query: 2   LSTSFNKLYSDLPLNPSPLLKFPFQHSAETLTSSQLKALVLSRFTQGKFFDLLQNVVASP 61
           LS+SFNKLYSD PL  SPLLK PFQHS +TLT  QL+ALVLSRF+QGKFFDLLQNVVASP
Sbjct: 16  LSSSFNKLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFSQGKFFDLLQNVVASP 75

Query: 62  SVLLTASQNLVTPPPSNGLNAPDSLPIVDLVSKCFSVEEMARELYENRFDVGACCVRMVP 121
           SVL TASQNL+TP PSN LNAPDSL  +D+VS CFSVE+MARELYENRFDVGACCVR+  
Sbjct: 76  SVLFTASQNLITPLPSNRLNAPDSLLSLDMVSSCFSVEDMARELYENRFDVGACCVRLES 135

Query: 122 SDEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKHSVQN 181
           S+EKGE LVLPNLKLKVL+EAIR+VLEIVYDERFVTFSYGGRVGMGRHTAIRYLK+SVQN
Sbjct: 136 SEEKGEFLVLPNLKLKVLLEAIRIVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQN 195

Query: 182 PSWWFTIAFHRNKFDSVHVNKLCLLVEEKIKDDILIYMLKKLFELEAIQIELGACYLGRG 241
           PSWWFT+AF R KFDSVHVNKLCLLV+EKIKDDILI MLKKLFELEA+QIELG CYLGRG
Sbjct: 196 PSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFELEAVQIELGGCYLGRG 255

Query: 242 FPQESGLCSILFNIYFNGFDKEIQQIRLQKNKENPKFNPVEMVSFHNPVKIYAVRYLDEI 301
            PQESGLCSIL NIYFNGFDKEIQQIRL+KN+ENPKF+    VSFHNPVKIYAVRYLDEI
Sbjct: 256 LPQESGLCSILSNIYFNGFDKEIQQIRLEKNEENPKFSLDGTVSFHNPVKIYAVRYLDEI 315

Query: 302 LVITSGSKMLTMDLKGQVLKYLERNLELEVDRMNSAIHSAVSEKIRFLGMELHAVPPSVL 361
           LVITSGSKML M+LK QVL+YLE NLELEVDRMN+AIHSAVSEKI FLGMEL AV PSVL
Sbjct: 316 LVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVLPSVL 375

Query: 362 HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTNGFKFKFQ 421
           HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLK+T+G K +FQ
Sbjct: 376 HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKRTDGLKSEFQ 435

Query: 422 IEEEVREIFSNWADEVVQDFFEYLEDYAEWHRVLLRGDFLHLKHIRNQLPEDLVNAYDRF 481
           IE+EV EIF NWADEVV+DFFE LED  EWHR L  GDFL LKHIRNQLP DLVNAYDRF
Sbjct: 436 IEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHIRNQLPVDLVNAYDRF 495

Query: 482 QDQVDKHLNPVKVKNEKAREDEEKRVEEEQQYAKRTVEDLRRLCIKVDAPIELVRNAVKM 541
           QDQV++HLNP+K K EKAREDEEKR+ EE++YAKRTVEDL RLCIKV+APIELVR AVKM
Sbjct: 496 QDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCIKVEAPIELVRKAVKM 555

Query: 542 VGFTNKMGRPQPISSLVALEDSDIIKWYAGVGRRWLDFFCCCDNYKMVKTVVTYHLRFSC 601
           +GFTNKMGRP+PISSL+ALED+DIIKWYAGVGRRWLDFFCCC NYKMVKT+VTYHLRFSC
Sbjct: 556 IGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTIVTYHLRFSC 615

Query: 602 ILTLAEKHESTKREAMKHYSKDLKVFDLKGNEEMHFPTEREVKMLGDRNLADPYPVDGAL 661
           ILTLAEKHESTKREAMKHYSKDLKVFDL G EEMHFPTEREVKMLG+RNLADPYPVDGA 
Sbjct: 616 ILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKMLGERNLADPYPVDGAF 675

Query: 662 SLFLIRLATDEPSYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGEKWIRGMGVMHESLN 721
           SLFLIRL TDE SYPCIAHFCNRTDSILYRVRLLQ+TLNVNPSNG +W+RGMGV+HESLN
Sbjct: 676 SLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNGVEWVRGMGVIHESLN 735

Query: 722 QRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 755
           QRCLPLCADHISDLYMGKINLQDLDCTLSLDMD
Sbjct: 736 QRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Sgr018086 vs. ExPASy Swiss-Prot
Match: Q9LZA5 (Nuclear intron maturase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT3 PE=3 SV=2)

HSP 1 Score: 832.0 bits (2148), Expect = 5.3e-240
Identity = 438/734 (59.67%), Postives = 540/734 (73.57%), Query Frame = 0

Query: 26  QHSAETLTSSQLKALVLSRFTQGKFFDLLQNVVASPSVLLTASQNLVTPPPSNGLNAPDS 85
           Q   E L  S+L+ALVL +++ GKF+ L++N V+ P VLL A QNL        L+A  S
Sbjct: 38  QTITEPLVKSELEALVLKQYSHGKFYSLVKNAVSLPCVLLAACQNL-------SLSANSS 97

Query: 86  LPIVDLVSKCFSVEEMARELYENRFDVGACCVRMVPSDEKGESLVLPNLKLKVLIEAIRM 145
             + D VS+ FS+EEM RE+ E RFD+ +CCV  + S     SLVLPNLKLKVLIEAIRM
Sbjct: 98  GDLADRVSRRFSIEEMGREIREGRFDIRSCCVEFISS-----SLVLPNLKLKVLIEAIRM 157

Query: 146 VLEIVYDERFVTFSYGGRVGMGRHTAIRYLKHSVQNPSWWFTIAFHRNKFDSVHVNKLCL 205
           VLEIVYD+RF TFSYGGRVGMGRHTAIRYLK+SV+NP WWF ++F R  F+  +V+ LC 
Sbjct: 158 VLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFEERNVDILCG 217

Query: 206 LVEEKIKDDILIYMLKKLFELEAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEIQ 265
            V EKI D +LI M+KKLFE   ++IELG C  GRGFPQE GLCSIL N+YF+G DKEIQ
Sbjct: 218 FVGEKINDVMLIEMIKKLFEFGILKIELGGCNSGRGFPQECGLCSILINVYFDGLDKEIQ 277

Query: 266 QIRLQKNKENPKFNPVEMVS----FHNPVKIYAVRYLDEILVITSGSKMLTMDLKGQVLK 325
            +RL+   +NP+    +  S    F  PV IYAVRYLDEILVITSGSKMLTMDLK +++ 
Sbjct: 278 DLRLKMKVKNPRVGTGDEESTGNVFFKPVNIYAVRYLDEILVITSGSKMLTMDLKKRIVD 337

Query: 326 YLERNLELEVDRMNSAIHSAVSEKIRFLGMELHAVPPSVLHPPMSEKAIRARKKYLRQKE 385
            LE+ LEL VDR+N++IHSAVSEKI FLGM L AVPPSVL PP SEKA+RA KKY RQK+
Sbjct: 338 ILEQRLELRVDRLNTSIHSAVSEKINFLGMYLQAVPPSVLRPPKSEKAVRAMKKYQRQKD 397

Query: 386 VRAIELRNARERNRKKLGLKILSHVFKKLKQTNGFKFKFQIEEEVREIFSNWADEVVQDF 445
           VR +ELRNARERNRK LGLKI  HV KK+KQ+NGFKF+ +IE EVR+IF +W +EV+QDF
Sbjct: 398 VRKLELRNARERNRKTLGLKIFRHVLKKIKQSNGFKFEGEIENEVRDIFQSWGEEVMQDF 457

Query: 446 FEYLEDYAEWHRVLLRGDFLHLKHIRNQLPEDLVNAYDRFQDQVDKHLNPVKVKNEKARE 505
              LE+  +WH +L RGDFL L+HIR +LP+DL++AYD FQ+QVDKHL P + K  K  E
Sbjct: 458 MGSLEERWKWHWLLTRGDFLSLRHIREKLPQDLIDAYDEFQEQVDKHLAPTQAK--KVLE 517

Query: 506 DEEKRV--EEEQQYAKRTVEDLRRLCIKVDAPIELVRNAVKMVGFTNKMGRPQPISSLVA 565
           DEE+RV  EEEQ+YA+RTVEDL +LC+KV AP ELVR A+K+VGFTN MGRP+PI  LV 
Sbjct: 518 DEERRVEEEEEQRYAERTVEDLTKLCMKVSAPEELVRKAIKLVGFTNSMGRPRPIIHLVT 577

Query: 566 LEDSDIIKWYAGVGRRWLDFFCCCDNYKMVKTVVTYHLRFSCILTLAEKHESTKREAMKH 625
           LEDSDIIKWYA                                    EKH STK+  ++H
Sbjct: 578 LEDSDIIKWYA----------------------------------RHEKHGSTKK-LIRH 637

Query: 626 YSKDLKVFDLKGNEEMHFPTEREVKMLGDRNLADPYPVDGALSLFLIRLATDEPSYPCIA 685
           Y+KDL+V DL G EE HFP+EREVKM+GD+NL+DP PVDG LSL LIRLA+DEP + C A
Sbjct: 638 YTKDLRVSDLDGREEAHFPSEREVKMMGDKNLSDPKPVDGTLSLLLIRLASDEPLHHCAA 697

Query: 686 HFCNRTDSILYRVRLLQRTLNVNPSNGEKWIRGMGVMHESLNQRCLPLCADHISDLYMGK 745
            FC R+D+I++RV LLQ  L++NP + EKW+ GMG +H +LN++CLPLC+ HISD+Y+GK
Sbjct: 698 SFCERSDTIMHRVHLLQNRLHINPLDEEKWVPGMGTIHSALNRKCLPLCSTHISDVYLGK 722

Query: 746 INLQDLDCTLSLDM 754
           I LQD+D +  +D+
Sbjct: 758 ITLQDVDSSSFIDL 722

BLAST of Sgr018086 vs. ExPASy Swiss-Prot
Match: Q9CA78 (Nuclear intron maturase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT4 PE=3 SV=2)

HSP 1 Score: 211.8 bits (538), Expect = 2.6e-53
Identity = 200/761 (26.28%), Postives = 331/761 (43.50%), Query Frame = 0

Query: 36  QLKALVLSRFTQGKFFDLLQNVVASPSVLLTASQNL-----VTPPPSNGLNAPDSLPIVD 95
           +LK  V  +   GKF DLL+ V+A P  L  A   +     V+    NG  A DS+    
Sbjct: 90  RLKKRVKEQCINGKFSDLLKKVIARPETLRDAYDCIRLNSNVSITERNGSVAFDSI---- 149

Query: 96  LVSKCFSVEEMARELYENRFDVGACCVRMVPSDEKGESLVLPNLKLKVLIEAIRMVLEIV 155
                      A EL    FDV +    +V  D+  E LVLP++ LKV+ EAIR+VLE+V
Sbjct: 150 -----------AEELSSGVFDVASNTFSIVARDKTKEVLVLPSVALKVVQEAIRIVLEVV 209

Query: 156 YDERFVTFSYGGRVGMGRHTAIRYLKHSVQNPSWWFTIAFHRNKFDSVHVNKLCLLVEEK 215
           +   F   S+  R G GR +A++Y+ +++    W FT++ ++    SV  N L ++ EEK
Sbjct: 210 FSPHFSKISHSCRSGRGRASALKYINNNISRSDWCFTLSLNKKLDVSVFENLLSVM-EEK 269

Query: 216 IKDDILIYMLKKLFELEAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEIQQI--- 275
           ++D  L  +L+ +FE   + +E G    G G PQE  L  +L NIY + FD E  +I   
Sbjct: 270 VEDSSLSILLRSMFEARVLNLEFGGFPKGHGLPQEGVLSRVLMNIYLDRFDHEFYRISMR 329

Query: 276 -----------------------RLQKNKENPKFNPVEMVSFHNPVKIYAVRYLDEILVI 335
                                  R Q  ++  K    + V+    +++Y  R++DEI   
Sbjct: 330 HEALGLDSKTDEDSPGSKLRSWFRRQAGEQGLKSTTEQDVA----LRVYCCRFMDEIYFS 389

Query: 336 TSGSKMLTMDLKGQVLKYLERNLELEVDRMNSAIHSAVSEKIRFLGMELHAVPPSVLHPP 395
            SG K +  D++ + + +L  +L L++           +  +R LG     V  +V   P
Sbjct: 390 VSGPKKVASDIRSEAIGFLRNSLHLDITDETDPSPCEATSGLRVLGT---LVRKNVRESP 449

Query: 396 MSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTNGFKFKFQIEE 455
            + KA+   K+ +R   + A++   A      ++G K L H  KK+K           E 
Sbjct: 450 -TVKAVHKLKEKVR---LFALQKEEAWTLGTVRIGKKWLGHGLKKVK-----------ES 509

Query: 456 EVREIF-SNWADEVVQDFFEYLEDYAEWHRVLLR--------------GDFLHLKH-IRN 515
           E++ +  SN     +    +   +   W+++LLR               +F+  KH +  
Sbjct: 510 EIKGLADSNSTLSQISCHRKAGMETDHWYKILLRIWMEDVLRTSADRSEEFVLSKHVVEP 569

Query: 516 QLPEDLVNAYDRFQDQVDKHLNPVKVKNEKAREDEEKRVEEEQQYAKRTVEDLRRLCIKV 575
            +P++L +A+ +FQ+    +     V +E A  +      +          D       V
Sbjct: 570 TVPQELRDAFYKFQNAAAAY-----VSSETANLEALLPCPQS--------HDRPVFFGDV 629

Query: 576 DAPIELVRNAVKMVGFTNKMGRPQPISSLVALEDSDIIKWYAGVGRRWLDFFCCCDNYKM 635
            AP   +   +   G     G  +  S L+ L+ + II WY+G+ RRW+ ++  C N+  
Sbjct: 630 VAPTNAIGRRLYRYGLITAKGYARSNSMLILLDTAQIIDWYSGLVRRWVIWYEGCSNFDE 689

Query: 636 VKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLKGNEEMHFPTEREVKMLGD 695
           +K ++   +R SCI TLA K+   + E  K    +L       + E     E+      D
Sbjct: 690 IKALIDNQIRMSCIRTLAAKYRIHENEIEKRLDLELSTIPSAEDIEQEIQHEKLDSPAFD 749

Query: 696 RNLADPYPV--DGALSLFLIRLATDEPSYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNG 747
           R+    Y +   G   L L RL ++     C    C+     +Y +  ++R         
Sbjct: 750 RDEHLTYGLSNSGLCLLSLARLVSESRPCNCFVIGCSMAAPAVYTLHAMER------QKF 793

BLAST of Sgr018086 vs. ExPASy Swiss-Prot
Match: P03876 (Putative COX1/OXI3 intron 2 protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=AI2 PE=4 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 1.3e-12
Identity = 68/294 (23.13%), Postives = 128/294 (43.54%), Query Frame = 0

Query: 98  VEEMARELYENRFDVGACCVRMVPSDEKG-ESLVLPNLKLKVLIEAIRMVLEIVYDERFV 157
           + ++++++  N F         +P    G   L + N + K++ E++RM+LEI+Y+  F 
Sbjct: 326 LNKLSKDINTNMFKFSPVRRVEIPKTSGGFRPLSVGNPREKIVQESMRMMLEIIYNNSFS 385

Query: 158 TFSYGGRVGMGRHTAIRYLKHSVQNPSWWFTIAFHRNKFDSVHVNKLCLLVEEKIKDDIL 217
            +S+G R  +   TAI   K+ +Q  +W+  +  ++  FD++  N L  ++ E+IKD   
Sbjct: 386 YYSHGFRPNLSCLTAIIQCKNYMQYCNWFIKVDLNK-CFDTIPHNMLINVLNERIKDKGF 445

Query: 218 IYMLKKLFELEAIQIELGACYLGRGFPQESGLCSILFNIYF--------NGFDKEIQQIR 277
           + +L KL     +           G PQ S +  IL NI+         N F+ E     
Sbjct: 446 MDLLYKLLRAGYVDKNNNYHNTTLGIPQGSVVSPILCNIFLDKLDKYLENKFENEFNTGN 505

Query: 278 LQKNKENPKFNPV-------------------------EMVSFHNPVKIYAVRYLDEILV 337
           +     NP +N +                          M S  +  + Y VRY D+I++
Sbjct: 506 MSNRGRNPIYNSLSSKIYRCKLLSEKLKLIRLRDHYQRNMGSDKSFKRAYFVRYADDIII 565

Query: 338 ITSGSKMLTMDLKGQVLKYLERNLELEVDRMNSAIHSAVSEKIRFLGMELHAVP 358
              GS     ++   +  +L+ NL + ++ M+ ++     E + FLG ++   P
Sbjct: 566 GVMGSHNDCKNILNDINNFLKENLGMSIN-MDKSVIKHSKEGVSFLGYDVKVTP 617

BLAST of Sgr018086 vs. ExPASy Swiss-Prot
Match: P0A3U0 (Group II intron-encoded protein LtrA OS=Lactococcus lactis subsp. cremoris OX=1359 GN=ltrA PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 3.2e-11
Identity = 71/276 (25.72%), Postives = 117/276 (42.39%), Query Frame = 0

Query: 122 SDEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKHSVQN 181
           + +K   L +P    K++ EA+R++LE +Y+  F   S+G R     HTA++ +K     
Sbjct: 93  NSKKMRPLGIPTFTDKLIQEAVRIILESIYEPVFEDVSHGFRPQRSCHTALKTIKREF-G 152

Query: 182 PSWWFTIAFHRNKFDSVHVNKLCLLVEEKIKD---DILIYMLKKLFELEAIQIELGACYL 241
            + WF     +  FD++    L  L+  KIKD     LIY   K   LE  Q        
Sbjct: 153 GARWFVEGDIKGCFDNIDHVTLIGLINLKIKDMKMSQLIYKFLKAGYLENWQYH----KT 212

Query: 242 GRGFPQESGLCSILFNIYFNGFDKEIQQIRLQKNKENPKFNPVEMVSFHNPVK------- 301
             G PQ   L  +L NIY +  DK + Q++++ ++E+P+    E    HN +K       
Sbjct: 213 YSGTPQGGILSPLLANIYLHELDKFVLQLKMKFDRESPERITPEYRELHNEIKRISHRLK 272

Query: 302 ---------------------------------IYAVRYLDEILVITSGSKMLTMDLKGQ 354
                                            +  VRY D+ ++   GSK     +K Q
Sbjct: 273 KLEGEEKAKVLLEYQEKRKRLPTLPCTSQTNKVLKYVRYADDFIISVKGSKEDCQWIKEQ 332

BLAST of Sgr018086 vs. ExPASy Swiss-Prot
Match: P0A3U1 (Group II intron-encoded protein LtrA OS=Lactococcus lactis subsp. cremoris (strain MG1363) OX=416870 GN=ltrA PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 3.2e-11
Identity = 71/276 (25.72%), Postives = 117/276 (42.39%), Query Frame = 0

Query: 122 SDEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKHSVQN 181
           + +K   L +P    K++ EA+R++LE +Y+  F   S+G R     HTA++ +K     
Sbjct: 93  NSKKMRPLGIPTFTDKLIQEAVRIILESIYEPVFEDVSHGFRPQRSCHTALKTIKREF-G 152

Query: 182 PSWWFTIAFHRNKFDSVHVNKLCLLVEEKIKD---DILIYMLKKLFELEAIQIELGACYL 241
            + WF     +  FD++    L  L+  KIKD     LIY   K   LE  Q        
Sbjct: 153 GARWFVEGDIKGCFDNIDHVTLIGLINLKIKDMKMSQLIYKFLKAGYLENWQYH----KT 212

Query: 242 GRGFPQESGLCSILFNIYFNGFDKEIQQIRLQKNKENPKFNPVEMVSFHNPVK------- 301
             G PQ   L  +L NIY +  DK + Q++++ ++E+P+    E    HN +K       
Sbjct: 213 YSGTPQGGILSPLLANIYLHELDKFVLQLKMKFDRESPERITPEYRELHNEIKRISHRLK 272

Query: 302 ---------------------------------IYAVRYLDEILVITSGSKMLTMDLKGQ 354
                                            +  VRY D+ ++   GSK     +K Q
Sbjct: 273 KLEGEEKAKVLLEYQEKRKRLPTLPCTSQTNKVLKYVRYADDFIISVKGSKEDCQWIKEQ 332

BLAST of Sgr018086 vs. ExPASy TrEMBL
Match: A0A6J1DNH6 (nuclear intron maturase 3, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111022853 PE=4 SV=1)

HSP 1 Score: 1321.6 bits (3419), Expect = 0.0e+00
Identity = 673/756 (89.02%), Postives = 706/756 (93.39%), Query Frame = 0

Query: 1   MLSTSFNKLYSDLPLNPS-PLLKFPFQHSAETLTSSQLKALVLSRFTQGKFFDLLQNVVA 60
           +LSTSFNKLYSDLPL  S PL  FPFQHS ETLT  +LKALVLSRFT GKF DLLQNVVA
Sbjct: 15  ILSTSFNKLYSDLPLKHSPPLHNFPFQHSTETLTWPELKALVLSRFTHGKFLDLLQNVVA 74

Query: 61  SPSVLLTASQNLVT-PPPSNGLNAPDSLPIVDLVSKCFSVEEMARELYENRFDVGACCVR 120
           SPSVLLTASQNL+T PPPSNGLNAPD LPI+DLVSKCFSVEEMARELYE+RFDVGACCVR
Sbjct: 75  SPSVLLTASQNLITPPPPSNGLNAPDPLPILDLVSKCFSVEEMARELYEDRFDVGACCVR 134

Query: 121 MVPSDEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKHS 180
           M   D+KG+SLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLK+S
Sbjct: 135 M---DQKGQSLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNS 194

Query: 181 VQNPSWWFTIAFHRNKFDSVHVNKLCLLVEEKIKDDILIYMLKKLFELEAIQIELGACYL 240
           VQNPSWWFT+AF R KFDSVHV+KLCLLVEEKIKD +LI MLKKLFELEAIQIELGACYL
Sbjct: 195 VQNPSWWFTVAFRRKKFDSVHVHKLCLLVEEKIKDHLLICMLKKLFELEAIQIELGACYL 254

Query: 241 GRGFPQESGLCSILFNIYFNGFDKEIQQIRLQKNKENPKFNPVEMVSFHNPVKIYAVRYL 300
           GRGFPQESGLCSIL NI+FNGFDK+IQQIRLQKN+ENPKF+  E+VSFH+PVKIYAVRYL
Sbjct: 255 GRGFPQESGLCSILCNIFFNGFDKDIQQIRLQKNEENPKFSLDEIVSFHSPVKIYAVRYL 314

Query: 301 DEILVITSGSKMLTMDLKGQVLKYLERNLELEVDRMNSAIHSAVSEKIRFLGMELHAVPP 360
           DEILVITSGSKMLTMDLK QVLKYLE NLELEVDRMN+AIHSAVSEKI FLGMEL AVPP
Sbjct: 315 DEILVITSGSKMLTMDLKSQVLKYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPP 374

Query: 361 SVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTNGFKF 420
           SVLHPPMSEKAIRARKKY+RQKEVR IELRNARERNRKKLGLKILSHVFKKLKQT+GFKF
Sbjct: 375 SVLHPPMSEKAIRARKKYIRQKEVRVIELRNARERNRKKLGLKILSHVFKKLKQTDGFKF 434

Query: 421 KFQIEEEVREIFSNWADEVVQDFFEYLEDYAEWHRVLLRGDFLHLKHIRNQLPEDLVNAY 480
           +FQIE+EVREIF NWADEV Q FFE LE++AEWH  L  GDFL LKHIRNQLPEDLVNAY
Sbjct: 435 EFQIEKEVREIFRNWADEVAQHFFESLENHAEWHHALSAGDFLSLKHIRNQLPEDLVNAY 494

Query: 481 DRFQDQVDKHLNPVKVKNEKAREDEEKRVEEEQQYAKRTVEDLRRLCIKVDAPIELVRNA 540
           DRFQDQVDKHLNPVKVK  KAREDEEKRVEEEQ+YA+RTVEDL RLCIKVDAPIELVR A
Sbjct: 495 DRFQDQVDKHLNPVKVKYVKAREDEEKRVEEEQKYARRTVEDLTRLCIKVDAPIELVRKA 554

Query: 541 VKMVGFTNKMGRPQPISSLVALEDSDIIKWYAGVGRRWLDFFCCCDNYKMVKTVVTYHLR 600
           VKMVGFTNKMGRPQPIS LVALED DIIKWYAGVGRRWLDFFCCC NYKMVKTVVTYHLR
Sbjct: 555 VKMVGFTNKMGRPQPISLLVALEDIDIIKWYAGVGRRWLDFFCCCHNYKMVKTVVTYHLR 614

Query: 601 FSCILTLAEKHESTKREAMKHYSKDLKVFDLKGNEEMHFPTEREVKMLGDRNLADPYPVD 660
           FSCILTLAEKHESTKREAMKH+SKDLKVFDL G+EE+HFPTEREVKMLGDR LADPYPVD
Sbjct: 615 FSCILTLAEKHESTKREAMKHFSKDLKVFDLNGDEEIHFPTEREVKMLGDRMLADPYPVD 674

Query: 661 GALSLFLIRLATDEPSYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGEKWIRGMGVMHE 720
           G LSLFLIRLA DEPS PCIAHFCNRTDSILYRVRLLQRTLNVN  +GEKW+RGMGV+HE
Sbjct: 675 GTLSLFLIRLAIDEPSCPCIAHFCNRTDSILYRVRLLQRTLNVNSCDGEKWVRGMGVIHE 734

Query: 721 SLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 755
           SLNQRCLPLCADHISDLYMG+INLQDLDCTLSLDMD
Sbjct: 735 SLNQRCLPLCADHISDLYMGRINLQDLDCTLSLDMD 767

BLAST of Sgr018086 vs. ExPASy TrEMBL
Match: A0A6J1ILC2 (nuclear intron maturase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111476629 PE=4 SV=1)

HSP 1 Score: 1306.2 bits (3379), Expect = 0.0e+00
Identity = 654/753 (86.85%), Postives = 697/753 (92.56%), Query Frame = 0

Query: 2   LSTSFNKLYSDLPLNPSPLLKFPFQHSAETLTSSQLKALVLSRFTQGKFFDLLQNVVASP 61
           LS+S NKLYSD PL  SPLLK PFQHS +TLT  QL+ALVLSRF+QGKFFDLLQNVVASP
Sbjct: 16  LSSSLNKLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFSQGKFFDLLQNVVASP 75

Query: 62  SVLLTASQNLVTPPPSNGLNAPDSLPIVDLVSKCFSVEEMARELYENRFDVGACCVRMVP 121
           SVL TASQNL+TP PSN LNAP+SL  +D+VS CFSVE+MARELYENRFDVGACCVR+  
Sbjct: 76  SVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELYENRFDVGACCVRLES 135

Query: 122 SDEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKHSVQN 181
           S+EKGE LVLPNLKLKVL+EA++MVLEIVYDERFVTFSYGGRVGMGRHTAIRYLK+SVQN
Sbjct: 136 SEEKGEFLVLPNLKLKVLLEAMKMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQN 195

Query: 182 PSWWFTIAFHRNKFDSVHVNKLCLLVEEKIKDDILIYMLKKLFELEAIQIELGACYLGRG 241
           PSWWFT+AF R KFDSVHVNKLCLLV+EKIKDDILI MLKKLFELEA+QIELG CYLGRG
Sbjct: 196 PSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFELEAVQIELGGCYLGRG 255

Query: 242 FPQESGLCSILFNIYFNGFDKEIQQIRLQKNKENPKFNPVEMVSFHNPVKIYAVRYLDEI 301
           FPQESGLCSIL NIYFNGFDKEIQQIRLQKN+ENPKF+    VSFHNPVKIYAVRYLDEI
Sbjct: 256 FPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLNGTVSFHNPVKIYAVRYLDEI 315

Query: 302 LVITSGSKMLTMDLKGQVLKYLERNLELEVDRMNSAIHSAVSEKIRFLGMELHAVPPSVL 361
           LVITSGSKM  M+LK QVL+YLE NLELEVDRMN+AIHSAVSEKI FLGMEL AVPPSVL
Sbjct: 316 LVITSGSKMQIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVL 375

Query: 362 HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTNGFKFKFQ 421
           HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLK+T+G K +FQ
Sbjct: 376 HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKRTDGLKSEFQ 435

Query: 422 IEEEVREIFSNWADEVVQDFFEYLEDYAEWHRVLLRGDFLHLKHIRNQLPEDLVNAYDRF 481
           IE+EV EIF NWADEVV+DFFE LED  EWHR L  GDFL LKHIRNQLP DLVNAYDRF
Sbjct: 436 IEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHIRNQLPVDLVNAYDRF 495

Query: 482 QDQVDKHLNPVKVKNEKAREDEEKRVEEEQQYAKRTVEDLRRLCIKVDAPIELVRNAVKM 541
           QDQV+KHLNP+K K EKAREDEEKR+ EE++YAKRTVEDL RLCIKV+APIELVR AVKM
Sbjct: 496 QDQVNKHLNPLKAKKEKAREDEEKRLVEEERYAKRTVEDLTRLCIKVEAPIELVRKAVKM 555

Query: 542 VGFTNKMGRPQPISSLVALEDSDIIKWYAGVGRRWLDFFCCCDNYKMVKTVVTYHLRFSC 601
           +GFTNKMGRPQPISSL+ALED+DIIKWYAGVGRRWLDFFCCC NYKMVKT+VTYHLRFSC
Sbjct: 556 IGFTNKMGRPQPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTIVTYHLRFSC 615

Query: 602 ILTLAEKHESTKREAMKHYSKDLKVFDLKGNEEMHFPTEREVKMLGDRNLADPYPVDGAL 661
           ILTLAEKHESTKREAMKHYSKDLKVFDL G EEMHFPTEREVKMLG+RNLADPYPVDGA 
Sbjct: 616 ILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKMLGERNLADPYPVDGAF 675

Query: 662 SLFLIRLATDEPSYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGEKWIRGMGVMHESLN 721
           SLFLIRL TDE SYPCIAHFCNRTDSILYRVRLLQ+TLNVNPSNG +W+RGMGV+HESLN
Sbjct: 676 SLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNGVEWVRGMGVIHESLN 735

Query: 722 QRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 755
           QRCLPLCADHISDLYMGKINLQDLDCTLSLDMD
Sbjct: 736 QRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Sgr018086 vs. ExPASy TrEMBL
Match: A0A6J1FV64 (nuclear intron maturase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111447574 PE=4 SV=1)

HSP 1 Score: 1302.7 bits (3370), Expect = 0.0e+00
Identity = 653/753 (86.72%), Postives = 697/753 (92.56%), Query Frame = 0

Query: 2   LSTSFNKLYSDLPLNPSPLLKFPFQHSAETLTSSQLKALVLSRFTQGKFFDLLQNVVASP 61
           LS+SFNKLYSD PL  SPLLK PFQHS +TLT  QL+ALVLSRF+QGKFFDLLQNVVASP
Sbjct: 16  LSSSFNKLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFSQGKFFDLLQNVVASP 75

Query: 62  SVLLTASQNLVTPPPSNGLNAPDSLPIVDLVSKCFSVEEMARELYENRFDVGACCVRMVP 121
           SVL TASQNL+TP PSN LNAPDSL  +D+VS CFSVE+MARELYENRFDVGACCVR+  
Sbjct: 76  SVLFTASQNLITPLPSNRLNAPDSLLSLDMVSSCFSVEDMARELYENRFDVGACCVRLES 135

Query: 122 SDEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKHSVQN 181
           S+EKGE LVLPNLKLKVL+EAIR+VLEIVYDERFVTFSYGGRVGMGRHTAIRYLK+SVQN
Sbjct: 136 SEEKGEFLVLPNLKLKVLLEAIRIVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQN 195

Query: 182 PSWWFTIAFHRNKFDSVHVNKLCLLVEEKIKDDILIYMLKKLFELEAIQIELGACYLGRG 241
           PSWWFT+AF R KFDSVHVNKLCLLV+EKIKDDILI MLKKLFELEA+QIELG CYLGRG
Sbjct: 196 PSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFELEAVQIELGGCYLGRG 255

Query: 242 FPQESGLCSILFNIYFNGFDKEIQQIRLQKNKENPKFNPVEMVSFHNPVKIYAVRYLDEI 301
            PQESGLCSIL NIYFNGFDKEIQQIRL+KN+ENPKF+    VSFHNPVKIYAVRYLDEI
Sbjct: 256 LPQESGLCSILSNIYFNGFDKEIQQIRLEKNEENPKFSLDGTVSFHNPVKIYAVRYLDEI 315

Query: 302 LVITSGSKMLTMDLKGQVLKYLERNLELEVDRMNSAIHSAVSEKIRFLGMELHAVPPSVL 361
           LVITSGSKML M+LK QVL+YLE NLELEVDRMN+AIHSAVSEKI FLGMEL AV PSVL
Sbjct: 316 LVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVLPSVL 375

Query: 362 HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTNGFKFKFQ 421
           HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLK+T+G K +FQ
Sbjct: 376 HPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKRTDGLKSEFQ 435

Query: 422 IEEEVREIFSNWADEVVQDFFEYLEDYAEWHRVLLRGDFLHLKHIRNQLPEDLVNAYDRF 481
           IE+EV EIF NWADEVV+DFFE LED  EWHR L  GDFL LKHIRNQLP DLVNAYDRF
Sbjct: 436 IEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHIRNQLPVDLVNAYDRF 495

Query: 482 QDQVDKHLNPVKVKNEKAREDEEKRVEEEQQYAKRTVEDLRRLCIKVDAPIELVRNAVKM 541
           QDQV++HLNP+K K EKAREDEEKR+ EE++YAKRTVEDL RLCIKV+APIELVR AVKM
Sbjct: 496 QDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCIKVEAPIELVRKAVKM 555

Query: 542 VGFTNKMGRPQPISSLVALEDSDIIKWYAGVGRRWLDFFCCCDNYKMVKTVVTYHLRFSC 601
           +GFTNKMGRP+PISSL+ALED+DIIKWYAGVGRRWLDFFCCC NYKMVKT+VTYHLRFSC
Sbjct: 556 IGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTIVTYHLRFSC 615

Query: 602 ILTLAEKHESTKREAMKHYSKDLKVFDLKGNEEMHFPTEREVKMLGDRNLADPYPVDGAL 661
           ILTLAEKHESTKREAMKHYSKDLKVFDL G EEMHFPTEREVKMLG+RNLADPYPVDGA 
Sbjct: 616 ILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKMLGERNLADPYPVDGAF 675

Query: 662 SLFLIRLATDEPSYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGEKWIRGMGVMHESLN 721
           SLFLIRL TDE SYPCIAHFCNRTDSILYRVRLLQ+TLNVNPSNG +W+RGMGV+HESLN
Sbjct: 676 SLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNGVEWVRGMGVIHESLN 735

Query: 722 QRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 755
           QRCLPLCADHISDLYMGKINLQDLDCTLSLDMD
Sbjct: 736 QRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Sgr018086 vs. ExPASy TrEMBL
Match: A0A1S4E4Y1 (uncharacterized protein LOC103501588 OS=Cucumis melo OX=3656 GN=LOC103501588 PE=4 SV=1)

HSP 1 Score: 1287.7 bits (3331), Expect = 0.0e+00
Identity = 653/752 (86.84%), Postives = 693/752 (92.15%), Query Frame = 0

Query: 3   STSFNKLYSDLPLNPSPLLKFPFQHSAETLTSSQLKALVLSRFTQGKFFDLLQNVVASPS 62
           S S NKLYS LPL    +L  P QHS E+LTSSQLKALVLSRF+ GKF DL QNVVASPS
Sbjct: 17  SNSLNKLYSHLPLR-KKVLNLPSQHSPESLTSSQLKALVLSRFSHGKFVDLFQNVVASPS 76

Query: 63  VLLTASQNLVTPPPSNGLNAPDSLPIVDLVSKCFSVEEMARELYENRFDVGACCVRMVPS 122
           VLLTAS+NL+TPP S   NAPDSLP+ DLVSKCFSVE MAREL ENRFDVGACCV M P 
Sbjct: 77  VLLTASRNLITPPFS---NAPDSLPLSDLVSKCFSVEVMARELSENRFDVGACCVPMAPL 136

Query: 123 DEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKHSVQNP 182
           +EKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLK+SVQNP
Sbjct: 137 EEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNP 196

Query: 183 SWWFTIAFHRNKFDSVHVNKLCLLVEEKIKDDILIYMLKKLFELEAIQIELGACYLGRGF 242
           SWWFT+AF R KF+SVHVN LCLL++EKIKDDILIYML+KLFE+EAIQIELG CYLGRGF
Sbjct: 197 SWWFTVAFRRKKFESVHVNTLCLLMQEKIKDDILIYMLRKLFEVEAIQIELGGCYLGRGF 256

Query: 243 PQESGLCSILFNIYFNGFDKEIQQIRLQKNKENPKFNPVEMVSFHNPVKIYAVRYLDEIL 302
           PQESGLCSIL NIYFNGFDKEIQ+IRLQKN+ENPKFN  E+VSFHNPVKIYAVRYLDEIL
Sbjct: 257 PQESGLCSILLNIYFNGFDKEIQRIRLQKNEENPKFNLDEIVSFHNPVKIYAVRYLDEIL 316

Query: 303 VITSGSKMLTMDLKGQVLKYLERNLELEVDRMNSAIHSAVSEKIRFLGMELHAVPPSVLH 362
           VITSGSKMLTM+LK QVLKYLE NLELEVDRMN+AIHSAVSEKI FLGMEL AV PSVLH
Sbjct: 317 VITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIGFLGMELQAVTPSVLH 376

Query: 363 PPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTNGFKFKFQI 422
           PPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH+FKK K+T GFK +FQI
Sbjct: 377 PPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHMFKKFKRTGGFKSEFQI 436

Query: 423 EEEVREIFSNWADEVVQDFFEYLEDYAEWHRVLLRGDFLHLKHIRNQLPEDLVNAYDRFQ 482
           E EVR IF NWADEVVQDFFE  ED+AEWHRVL  GDFL LKHIRNQLPEDLVNAYDRFQ
Sbjct: 437 ETEVRSIFRNWADEVVQDFFESSEDHAEWHRVLSAGDFLSLKHIRNQLPEDLVNAYDRFQ 496

Query: 483 DQVDKHLNPVKVKNEKAREDEEKRVEEEQQYAKRTVEDLRRLCIKVDAPIELVRNAVKMV 542
            QV+KHLNPVKVK EKAREDEEKR+EEE+ YAKRTVEDL RLCIKVDAPIELVR AV+MV
Sbjct: 497 YQVNKHLNPVKVKKEKAREDEEKRLEEEELYAKRTVEDLTRLCIKVDAPIELVRKAVRMV 556

Query: 543 GFTNKMGRPQPISSLVALEDSDIIKWYAGVGRRWLDFFCCCDNYKMVKTVVTYHLRFSCI 602
           GFTNKMGRP+PISSL+ALED+DIIKWY+GVGRRWLDFFCCC NYKMVKTVVTYHLRFSCI
Sbjct: 557 GFTNKMGRPRPISSLIALEDADIIKWYSGVGRRWLDFFCCCHNYKMVKTVVTYHLRFSCI 616

Query: 603 LTLAEKHESTKREAMKHYSKDLKVFDLKGNEEMHFPTEREVKMLGDRNLADPYPVDGALS 662
           LTLAEKHESTKREAMKHY KDLKVFDL GNEEMHFPTE+ VKMLG+RNLADPYPVDGALS
Sbjct: 617 LTLAEKHESTKREAMKHYGKDLKVFDLNGNEEMHFPTEKAVKMLGERNLADPYPVDGALS 676

Query: 663 LFLIRLATDEPSYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGEKWIRGMGVMHESLNQ 722
           L LIRLATDE SYPCIA+FCNRT+SILYRVRLLQRTLNVNPS+G +W++GMGV+HESLNQ
Sbjct: 677 LLLIRLATDEASYPCIANFCNRTNSILYRVRLLQRTLNVNPSDGVEWVKGMGVIHESLNQ 736

Query: 723 RCLPLCADHISDLYMGKINLQDLDCTLSLDMD 755
           RCLPLCADHISDLYMGKINLQDLDCTLSLDMD
Sbjct: 737 RCLPLCADHISDLYMGKINLQDLDCTLSLDMD 764

BLAST of Sgr018086 vs. ExPASy TrEMBL
Match: A0A5A7SWY5 (RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold400G00320 PE=4 SV=1)

HSP 1 Score: 1286.9 bits (3329), Expect = 0.0e+00
Identity = 654/752 (86.97%), Postives = 692/752 (92.02%), Query Frame = 0

Query: 3   STSFNKLYSDLPLNPSPLLKFPFQHSAETLTSSQLKALVLSRFTQGKFFDLLQNVVASPS 62
           S S NKLYS LPL    +L  P QHS E+LTSSQLKALVLSRF+ GKF DL QNVVASPS
Sbjct: 17  SNSLNKLYSHLPLR-KKVLNLPSQHSPESLTSSQLKALVLSRFSHGKFVDLFQNVVASPS 76

Query: 63  VLLTASQNLVTPPPSNGLNAPDSLPIVDLVSKCFSVEEMARELYENRFDVGACCVRMVPS 122
           VLLTAS+NL+TPP S   NAPDSLP+ DLVSKCFSVE MAREL ENRFDVGACCV M P 
Sbjct: 77  VLLTASRNLITPPFS---NAPDSLPLSDLVSKCFSVEVMARELSENRFDVGACCVPMAPL 136

Query: 123 DEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKHSVQNP 182
           +EKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLK+SVQNP
Sbjct: 137 EEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNP 196

Query: 183 SWWFTIAFHRNKFDSVHVNKLCLLVEEKIKDDILIYMLKKLFELEAIQIELGACYLGRGF 242
           SWWFT+AF R KF+SVHVN LCLL++EKIKDDILIYML+KLFE+EAIQIELG CYLGRGF
Sbjct: 197 SWWFTVAFRRKKFESVHVNTLCLLMQEKIKDDILIYMLRKLFEVEAIQIELGGCYLGRGF 256

Query: 243 PQESGLCSILFNIYFNGFDKEIQQIRLQKNKENPKFNPVEMVSFHNPVKIYAVRYLDEIL 302
           PQESGLCSIL NIYFNGFDKEIQ+IRLQKN+ENPKFN  E+VSFHNPVKIYAVRYLDEIL
Sbjct: 257 PQESGLCSILLNIYFNGFDKEIQRIRLQKNEENPKFNLDEIVSFHNPVKIYAVRYLDEIL 316

Query: 303 VITSGSKMLTMDLKGQVLKYLERNLELEVDRMNSAIHSAVSEKIRFLGMELHAVPPSVLH 362
           VITSGSKMLTM+LK QVLKYLE NLELEVDRMN+AIHSAVSEKI FLGMEL AV PSVLH
Sbjct: 317 VITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIGFLGMELQAVTPSVLH 376

Query: 363 PPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTNGFKFKFQI 422
           PPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKK K+T GFK +FQI
Sbjct: 377 PPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKFKRTGGFKSEFQI 436

Query: 423 EEEVREIFSNWADEVVQDFFEYLEDYAEWHRVLLRGDFLHLKHIRNQLPEDLVNAYDRFQ 482
           E EVR IF NWADEVVQDFFE  ED AEWHRVL  GDFL LKHIRNQLPEDLVNAYDRFQ
Sbjct: 437 ETEVRSIFRNWADEVVQDFFESSEDPAEWHRVLSAGDFLSLKHIRNQLPEDLVNAYDRFQ 496

Query: 483 DQVDKHLNPVKVKNEKAREDEEKRVEEEQQYAKRTVEDLRRLCIKVDAPIELVRNAVKMV 542
            QV+KHLNPVKVK EKAREDEEKR+EEE+ YAKRTVEDL RLCIKVDAPIELVR AV+MV
Sbjct: 497 YQVNKHLNPVKVKKEKAREDEEKRLEEEELYAKRTVEDLTRLCIKVDAPIELVRKAVRMV 556

Query: 543 GFTNKMGRPQPISSLVALEDSDIIKWYAGVGRRWLDFFCCCDNYKMVKTVVTYHLRFSCI 602
           GFTNKMGRP+PISSL+ALED+DIIKWY+GVGRRWLDFFCCC NYKMVKTVVTYHLRFSCI
Sbjct: 557 GFTNKMGRPRPISSLIALEDADIIKWYSGVGRRWLDFFCCCHNYKMVKTVVTYHLRFSCI 616

Query: 603 LTLAEKHESTKREAMKHYSKDLKVFDLKGNEEMHFPTEREVKMLGDRNLADPYPVDGALS 662
           LTLAEKHESTKREAMKHY KDLKVFDL GNEEMHFPTE+ VKMLG+RNLADPYPVDGALS
Sbjct: 617 LTLAEKHESTKREAMKHYGKDLKVFDLNGNEEMHFPTEKAVKMLGERNLADPYPVDGALS 676

Query: 663 LFLIRLATDEPSYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGEKWIRGMGVMHESLNQ 722
           L LIRLATDE SYPCIA+FCNRT+SILYRVRLLQRTLNVNPS+G +W++GMGV+HESLNQ
Sbjct: 677 LLLIRLATDEASYPCIANFCNRTNSILYRVRLLQRTLNVNPSDGVEWVKGMGVIHESLNQ 736

Query: 723 RCLPLCADHISDLYMGKINLQDLDCTLSLDMD 755
           RCLPLCADHISDLYMGKINLQDLDCTLSLDMD
Sbjct: 737 RCLPLCADHISDLYMGKINLQDLDCTLSLDMD 764

BLAST of Sgr018086 vs. TAIR 10
Match: AT5G04050.2 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 741.5 bits (1913), Expect = 6.7e-214
Identity = 403/734 (54.90%), Postives = 498/734 (67.85%), Query Frame = 0

Query: 26  QHSAETLTSSQLKALVLSRFTQGKFFDLLQNVVASPSVLLTASQNLVTPPPSNGLNAPDS 85
           Q   E L  S+L+ALVL +++ GKF+ L++N V+ P VLL A QNL        L+A  S
Sbjct: 38  QTITEPLVKSELEALVLKQYSHGKFYSLVKNAVSLPCVLLAACQNL-------SLSANSS 97

Query: 86  LPIVDLVSKCFSVEEMARELYENRFDVGACCVRMVPSDEKGESLVLPNLKLKVLIEAIRM 145
             + D VS+ FS+EEM RE+ E RFD+ +CCV  + S     SLVLPNLKLKVLIEAIRM
Sbjct: 98  GDLADRVSRRFSIEEMGREIREGRFDIRSCCVEFISS-----SLVLPNLKLKVLIEAIRM 157

Query: 146 VLEIVYDERFVTFSYGGRVGMGRHTAIRYLKHSVQNPSWWFTIAFHRNKFDSVHVNKLCL 205
           VLEIVYD+RF TFSYGGRVGMGRHTAIRYLK+SV+NP WWF ++F R  F+  +V+ LC 
Sbjct: 158 VLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFEERNVDILCG 217

Query: 206 LVEEKIKDDILIYMLKKLFELEAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEIQ 265
            V EKI D +LI M+KKLFE   ++IELG C  GRGFPQE GLCSIL N+YF+G DKEIQ
Sbjct: 218 FVGEKINDVMLIEMIKKLFEFGILKIELGGCNSGRGFPQECGLCSILINVYFDGLDKEIQ 277

Query: 266 QIRLQKNKENPKFNPVEMVS----FHNPVKIYAVRYLDEILVITSGSKMLTMDLKGQVLK 325
            +RL+   +NP+    +  S    F  PV IYAVRYLDEILVITSGSKMLTMDLK +++ 
Sbjct: 278 DLRLKMKVKNPRVGTGDEESTGNVFFKPVNIYAVRYLDEILVITSGSKMLTMDLKKRIVD 337

Query: 326 YLERNLELEVDRMNSAIHSAVSEKIRFLGMELHAVPPSVLHPPMSEKAIRARKKYLRQKE 385
            LE+ LEL VDR+N++IHSAVSEKI FLGM L AVPPSVL PP SEKA+RA KKY RQK+
Sbjct: 338 ILEQRLELRVDRLNTSIHSAVSEKINFLGMYLQAVPPSVLRPPKSEKAVRAMKKYQRQKD 397

Query: 386 VRAIELRNARERNRKKLGLKILSHVFKKLKQTNGFKFKFQIEEEVREIFSNWADEVVQDF 445
           VR +ELRNARERNRK LGLKI  HV KK+KQ+NGFKF+ +IE EVR+IF +W +EV+QDF
Sbjct: 398 VRKLELRNARERNRKTLGLKIFRHVLKKIKQSNGFKFEGEIENEVRDIFQSWGEEVMQDF 457

Query: 446 FEYLEDYAEWHRVLLRGDFLHLKHIRNQLPEDLVNAYDRFQDQVDKHLNPVKVKNEKARE 505
              LE+  +WH +L RGDFL L+HIR +LP+DL++AYD FQ+QVDKHL P + K  K  E
Sbjct: 458 MGSLEERWKWHWLLTRGDFLSLRHIREKLPQDLIDAYDEFQEQVDKHLAPTQAK--KVLE 517

Query: 506 DEEKRV--EEEQQYAKRTVEDLRRLCIKVDAPIELVRNAVKMVGFTNKMGRPQPISSLVA 565
           DEE+RV  EEEQ+YA+RTVEDL +LC+KV AP ELVR A+                    
Sbjct: 518 DEERRVEEEEEQRYAERTVEDLTKLCMKVSAPEELVRKAI-------------------- 577

Query: 566 LEDSDIIKWYAGVGRRWLDFFCCCDNYKMVKTVVTYHLRFSCILTLAEKHESTKREAMKH 625
                                                                       
Sbjct: 578 ------------------------------------------------------------ 637

Query: 626 YSKDLKVFDLKGNEEMHFPTEREVKMLGDRNLADPYPVDGALSLFLIRLATDEPSYPCIA 685
                KV DL G EE HFP+EREVKM+GD+NL+DP PVDG LSL LIRLA+DEP + C A
Sbjct: 638 -----KVSDLDGREEAHFPSEREVKMMGDKNLSDPKPVDGTLSLLLIRLASDEPLHHCAA 672

Query: 686 HFCNRTDSILYRVRLLQRTLNVNPSNGEKWIRGMGVMHESLNQRCLPLCADHISDLYMGK 745
            FC R+D+I++RV LLQ  L++NP + EKW+ GMG +H +LN++CLPLC+ HISD+Y+GK
Sbjct: 698 SFCERSDTIMHRVHLLQNRLHINPLDEEKWVPGMGTIHSALNRKCLPLCSTHISDVYLGK 672

Query: 746 INLQDLDCTLSLDM 754
           I LQD+D +  +D+
Sbjct: 758 ITLQDVDSSSFIDL 672

BLAST of Sgr018086 vs. TAIR 10
Match: AT5G04050.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 702.6 bits (1812), Expect = 3.4e-202
Identity = 368/574 (64.11%), Postives = 443/574 (77.18%), Query Frame = 0

Query: 26  QHSAETLTSSQLKALVLSRFTQGKFFDLLQNVVASPSVLLTASQNLVTPPPSNGLNAPDS 85
           Q   E L  S+L+ALVL +++ GKF+ L++N V+ P VLL A QNL        L+A  S
Sbjct: 38  QTITEPLVKSELEALVLKQYSHGKFYSLVKNAVSLPCVLLAACQNL-------SLSANSS 97

Query: 86  LPIVDLVSKCFSVEEMARELYENRFDVGACCVRMVPSDEKGESLVLPNLKLKVLIEAIRM 145
             + D VS+ FS+EEM RE+ E RFD+ +CCV  + S     SLVLPNLKLKVLIEAIRM
Sbjct: 98  GDLADRVSRRFSIEEMGREIREGRFDIRSCCVEFISS-----SLVLPNLKLKVLIEAIRM 157

Query: 146 VLEIVYDERFVTFSYGGRVGMGRHTAIRYLKHSVQNPSWWFTIAFHRNKFDSVHVNKLCL 205
           VLEIVYD+RF TFSYGGRVGMGRHTAIRYLK+SV+NP WWF ++F R  F+  +V+ LC 
Sbjct: 158 VLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFEERNVDILCG 217

Query: 206 LVEEKIKDDILIYMLKKLFELEAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEIQ 265
            V EKI D +LI M+KKLFE   ++IELG C  GRGFPQE GLCSIL N+YF+G DKEIQ
Sbjct: 218 FVGEKINDVMLIEMIKKLFEFGILKIELGGCNSGRGFPQECGLCSILINVYFDGLDKEIQ 277

Query: 266 QIRLQKNKENPKFNPVEMVS----FHNPVKIYAVRYLDEILVITSGSKMLTMDLKGQVLK 325
            +RL+   +NP+    +  S    F  PV IYAVRYLDEILVITSGSKMLTMDLK +++ 
Sbjct: 278 DLRLKMKVKNPRVGTGDEESTGNVFFKPVNIYAVRYLDEILVITSGSKMLTMDLKKRIVD 337

Query: 326 YLERNLELEVDRMNSAIHSAVSEKIRFLGMELHAVPPSVLHPPMSEKAIRARKKYLRQKE 385
            LE+ LEL VDR+N++IHSAVSEKI FLGM L AVPPSVL PP SEKA+RA KKY RQK+
Sbjct: 338 ILEQRLELRVDRLNTSIHSAVSEKINFLGMYLQAVPPSVLRPPKSEKAVRAMKKYQRQKD 397

Query: 386 VRAIELRNARERNRKKLGLKILSHVFKKLKQTNGFKFKFQIEEEVREIFSNWADEVVQDF 445
           VR +ELRNARERNRK LGLKI  HV KK+KQ+NGFKF+ +IE EVR+IF +W +EV+QDF
Sbjct: 398 VRKLELRNARERNRKTLGLKIFRHVLKKIKQSNGFKFEGEIENEVRDIFQSWGEEVMQDF 457

Query: 446 FEYLEDYAEWHRVLLRGDFLHLKHIRNQLPEDLVNAYDRFQDQVDKHLNPVKVKNEKARE 505
              LE+  +WH +L RGDFL L+HIR +LP+DL++AYD FQ+QVDKHL P + K  K  E
Sbjct: 458 MGSLEERWKWHWLLTRGDFLSLRHIREKLPQDLIDAYDEFQEQVDKHLAPTQAK--KVLE 517

Query: 506 DEEKRV--EEEQQYAKRTVEDLRRLCIKVDAPIELVRNAVKMVGFTNKMGRPQPISSLVA 565
           DEE+RV  EEEQ+YA+RTVEDL +LC+KV AP ELVR A+K+VGFTN MGRP+PI  LV 
Sbjct: 518 DEERRVEEEEEQRYAERTVEDLTKLCMKVSAPEELVRKAIKLVGFTNSMGRPRPIIHLVT 577

Query: 566 LEDSDIIKWYAGVGRRWLDFFCCCDNYKMVKTVV 594
           LEDSDIIKWYAGVGR+WLDFFCCC NYKMVK++V
Sbjct: 578 LEDSDIIKWYAGVGRKWLDFFCCCHNYKMVKSLV 597

BLAST of Sgr018086 vs. TAIR 10
Match: AT1G74350.1 (Intron maturase, type II family protein )

HSP 1 Score: 211.8 bits (538), Expect = 1.8e-54
Identity = 200/761 (26.28%), Postives = 331/761 (43.50%), Query Frame = 0

Query: 36  QLKALVLSRFTQGKFFDLLQNVVASPSVLLTASQNL-----VTPPPSNGLNAPDSLPIVD 95
           +LK  V  +   GKF DLL+ V+A P  L  A   +     V+    NG  A DS+    
Sbjct: 45  RLKKRVKEQCINGKFSDLLKKVIARPETLRDAYDCIRLNSNVSITERNGSVAFDSI---- 104

Query: 96  LVSKCFSVEEMARELYENRFDVGACCVRMVPSDEKGESLVLPNLKLKVLIEAIRMVLEIV 155
                      A EL    FDV +    +V  D+  E LVLP++ LKV+ EAIR+VLE+V
Sbjct: 105 -----------AEELSSGVFDVASNTFSIVARDKTKEVLVLPSVALKVVQEAIRIVLEVV 164

Query: 156 YDERFVTFSYGGRVGMGRHTAIRYLKHSVQNPSWWFTIAFHRNKFDSVHVNKLCLLVEEK 215
           +   F   S+  R G GR +A++Y+ +++    W FT++ ++    SV  N L ++ EEK
Sbjct: 165 FSPHFSKISHSCRSGRGRASALKYINNNISRSDWCFTLSLNKKLDVSVFENLLSVM-EEK 224

Query: 216 IKDDILIYMLKKLFELEAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEIQQI--- 275
           ++D  L  +L+ +FE   + +E G    G G PQE  L  +L NIY + FD E  +I   
Sbjct: 225 VEDSSLSILLRSMFEARVLNLEFGGFPKGHGLPQEGVLSRVLMNIYLDRFDHEFYRISMR 284

Query: 276 -----------------------RLQKNKENPKFNPVEMVSFHNPVKIYAVRYLDEILVI 335
                                  R Q  ++  K    + V+    +++Y  R++DEI   
Sbjct: 285 HEALGLDSKTDEDSPGSKLRSWFRRQAGEQGLKSTTEQDVA----LRVYCCRFMDEIYFS 344

Query: 336 TSGSKMLTMDLKGQVLKYLERNLELEVDRMNSAIHSAVSEKIRFLGMELHAVPPSVLHPP 395
            SG K +  D++ + + +L  +L L++           +  +R LG     V  +V   P
Sbjct: 345 VSGPKKVASDIRSEAIGFLRNSLHLDITDETDPSPCEATSGLRVLGT---LVRKNVRESP 404

Query: 396 MSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTNGFKFKFQIEE 455
            + KA+   K+ +R   + A++   A      ++G K L H  KK+K           E 
Sbjct: 405 -TVKAVHKLKEKVR---LFALQKEEAWTLGTVRIGKKWLGHGLKKVK-----------ES 464

Query: 456 EVREIF-SNWADEVVQDFFEYLEDYAEWHRVLLR--------------GDFLHLKH-IRN 515
           E++ +  SN     +    +   +   W+++LLR               +F+  KH +  
Sbjct: 465 EIKGLADSNSTLSQISCHRKAGMETDHWYKILLRIWMEDVLRTSADRSEEFVLSKHVVEP 524

Query: 516 QLPEDLVNAYDRFQDQVDKHLNPVKVKNEKAREDEEKRVEEEQQYAKRTVEDLRRLCIKV 575
            +P++L +A+ +FQ+    +     V +E A  +      +          D       V
Sbjct: 525 TVPQELRDAFYKFQNAAAAY-----VSSETANLEALLPCPQS--------HDRPVFFGDV 584

Query: 576 DAPIELVRNAVKMVGFTNKMGRPQPISSLVALEDSDIIKWYAGVGRRWLDFFCCCDNYKM 635
            AP   +   +   G     G  +  S L+ L+ + II WY+G+ RRW+ ++  C N+  
Sbjct: 585 VAPTNAIGRRLYRYGLITAKGYARSNSMLILLDTAQIIDWYSGLVRRWVIWYEGCSNFDE 644

Query: 636 VKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLKGNEEMHFPTEREVKMLGD 695
           +K ++   +R SCI TLA K+   + E  K    +L       + E     E+      D
Sbjct: 645 IKALIDNQIRMSCIRTLAAKYRIHENEIEKRLDLELSTIPSAEDIEQEIQHEKLDSPAFD 704

Query: 696 RNLADPYPV--DGALSLFLIRLATDEPSYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNG 747
           R+    Y +   G   L L RL ++     C    C+     +Y +  ++R         
Sbjct: 705 RDEHLTYGLSNSGLCLLSLARLVSESRPCNCFVIGCSMAAPAVYTLHAMER------QKF 748

BLAST of Sgr018086 vs. TAIR 10
Match: ATMG00520.1 (Intron maturase, type II family protein )

HSP 1 Score: 76.3 bits (186), Expect = 1.2e-13
Identity = 89/365 (24.38%), Postives = 161/365 (44.11%), Query Frame = 0

Query: 287 HNPVKIYAVRYLDEILVITSGSKMLTMDLKGQVLKYLERNLELEVDRMNSAIHSAVSEKI 346
           H  ++I   RY D++L+   G+  L ++++ ++  +L+  L L V    S   +A S  +
Sbjct: 311 HYLIRICYARYADDLLLGIVGAVELLIEIQKRIAHFLQSGLNLWVGSAGSTTIAARS-TV 370

Query: 347 RFLGMELHAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKI-LSH 406
            FLG  +  VPP    P    + +  R +   +  + A  LR+A     + LG  I +  
Sbjct: 371 EFLGTVIREVPPRTT-PIQFLRELEKRLRVKHRIHITACHLRSAIHSKFRNLGDSIPIKQ 430

Query: 407 VFKKLKQTNGFKFKFQIEEEVREIFSNWADEVVQDFFEYLEDYAEWHRVLLRGDFLHLKH 466
           + K + +T   +   Q+ E +    +      V   +  ++   +  R +    FLH   
Sbjct: 431 LTKGMSKTGSLQDGVQLAETLGT--AGVRSPQVSVLWGTVKHIRQGSRGI---SFLHSSG 490

Query: 467 IRNQLPEDLVNAYDRFQDQVDKHLNPVKVKNEKAREDEEKRVEEEQQYAKRTVEDLRRLC 526
            R+    D+     R       H   + +     R    K   E   +   ++       
Sbjct: 491 -RSNASSDVQQVVSR----SGTHARKLSLYTPPGR----KAAGEGGGHWAGSISS--EFP 550

Query: 527 IKVDAPIELVRNAVKMVGFTNKMGRPQPI--SSLVALEDSDIIKWYAGVGRRWLDFFCCC 586
           IK++API+ +   ++  G  ++  RP PI  + L  + D DI+ W AG+    L ++ C 
Sbjct: 551 IKIEAPIKKILRRLRDRGIISRR-RPWPIHVACLTNVSDEDIVNWSAGIAISPLSYYRCR 610

Query: 587 DNYKMVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLKGNEEM-HFPTERE 646
           DN   V+T+V + +R+S I TLA KH+S+    +  YSKD  + + +G + +  FP   E
Sbjct: 611 DNLYQVRTIVDHQIRWSAIFTLAHKHKSSAPNIILKYSKDSNIVNQEGGKILAEFPNSIE 656

Query: 647 VKMLG 648
           +  LG
Sbjct: 671 LGKLG 656

BLAST of Sgr018086 vs. TAIR 10
Match: ATCG00040.1 (maturase K )

HSP 1 Score: 43.9 bits (102), Expect = 6.7e-04
Identity = 31/112 (27.68%), Postives = 52/112 (46.43%), Query Frame = 0

Query: 526 IKVDA--PIELVRNAVKMVGFTNKMGRPQPISSLVALEDSDIIKWYAGVGRRWLDFFCCC 585
           IK+D+  PI  +  ++    F N +G P   ++     DSDI+  +  + R    ++   
Sbjct: 381 IKLDSKIPISSIIGSLAKDKFCNVLGHPISKATWTDSSDSDILNRFVRICRNISHYYSGS 440

Query: 586 DNYKMVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLKGNEEM 636
              K +   + Y LR  C+ TLA KH+ST R  +K     L    L G +++
Sbjct: 441 SKKKNLYR-IKYILRLCCVKTLARKHKSTVRTFLKRLGSGLLEEFLTGEDQV 491