Sgr017162 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr017162 vs. NCBI nr
Match: XP_038882989.1 (IST1-like protein [Benincasa hispida])

HSP 1 Score: 667.9 bits (1722), Expect = 1.1e-187
Identity = 372/458 (81.22%), Postives = 387/458 (84.50%), Query Frame = 0

Query: 357 MGAASEAAARSIKIVKLVVSLLRRGFNSSKWFCYFLFLLFLAALTNPFCYRLFSLVLDSK 416
           M AA+EAAARS+KIVK  ++LLRRGFNSSK                             K
Sbjct: 1   MSAATEAAARSVKIVKQFITLLRRGFNSSK----------------------------CK 60

Query: 417 TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEII 476
           TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEII
Sbjct: 61  TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEII 120

Query: 477 ELFCELVVARLSIIAKQRS-----KEGVASLIFAAPRCSEIPELSGLRKVFEKKYGKDFV 536
           ELFCELVVARLSIIAKQR      KEGVASLIFAAPRCSEIPELS LR VFEKKYGKDFV
Sbjct: 121 ELFCELVVARLSIIAKQRQCPADLKEGVASLIFAAPRCSEIPELSALRNVFEKKYGKDFV 180

Query: 537 SAATDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHQIEWDTTESEKELLKPPEE 596
           SAA DLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEH+IEWDTTESEKELLKPPEE
Sbjct: 181 SAAIDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHKIEWDTTESEKELLKPPEE 240

Query: 597 LIEGPRTFVSAASLPVKPVARQSAGDNAQFGRTANSRDDGSMHFQDTASAAEAAAKSAKQ 656
           LIEGPRTFVSAASLPVKPV   SAGDNAQ  RT NSR+D SMHFQD+ASAAEAAAK+AKQ
Sbjct: 241 LIEGPRTFVSAASLPVKPVVSHSAGDNAQNERTRNSREDESMHFQDSASAAEAAAKAAKQ 300

Query: 657 AIAAAQAAAYLANKDLNRDVRGSGFDLGFEQGPPANSTPTNSHNMDHQFRAGEERTTPPQ 716
           AIAAA+AAAYLANKDLNRD + SGF LGF  G PANSTPTNSHNMDH+F+ GEERTTP Q
Sbjct: 301 AIAAAEAAAYLANKDLNRDAQDSGFGLGF-GGSPANSTPTNSHNMDHRFKTGEERTTPTQ 360

Query: 717 SFGRCRSLKNDETRHVNTDYEMAYRRHSYNPTPSAHTDIKFDESDCEEEGTQTEEAGGGA 776
           SFGRC SLKN++T +VNTDYEMAYRRHSYNP     TDIKFDESDCEEE    E AG G 
Sbjct: 361 SFGRCSSLKNEDTGNVNTDYEMAYRRHSYNP-----TDIKFDESDCEEETQMDEGAGRGV 420

Query: 777 YRPPDRNPPPVPSSRVHPKLPDYDTLAARFEALKYRKT 810
            RPPDRNPPPVPSSRVHPKLPDYDTLAARFEALKYRKT
Sbjct: 421 SRPPDRNPPPVPSSRVHPKLPDYDTLAARFEALKYRKT 424

BLAST of Sgr017162 vs. NCBI nr
Match: XP_004134834.1 (IST1-like protein isoform X2 [Cucumis sativus] >KGN48972.1 hypothetical protein Csa_004187 [Cucumis sativus])

HSP 1 Score: 647.1 bits (1668), Expect = 2.0e-181
Identity = 360/457 (78.77%), Postives = 381/457 (83.37%), Query Frame = 0

Query: 357 MGAASEAAARSIKIVKLVVSLLRRGFNSSKWFCYFLFLLFLAALTNPFCYRLFSLVLDSK 416
           M AA+EAAARS+KIVK  +++LR GFNSSK                             K
Sbjct: 1   MSAATEAAARSLKIVKQFITILRCGFNSSK----------------------------CK 60

Query: 417 TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEII 476
           TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEII
Sbjct: 61  TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEII 120

Query: 477 ELFCELVVARLSIIAKQRS-----KEGVASLIFAAPRCSEIPELSGLRKVFEKKYGKDFV 536
           ELFCELVVARLSIIAKQR      KEGVASLIFAAPRCSEIPELS LR VFEKKYGKDFV
Sbjct: 121 ELFCELVVARLSIIAKQRQCPADLKEGVASLIFAAPRCSEIPELSALRNVFEKKYGKDFV 180

Query: 537 SAATDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHQIEWDTTESEKELLKPPEE 596
           SAA DLRPNCGVNRLLIDKLSVRTPTG+VKLKIMKEIAKEH+IEWDTTESEKELLKP EE
Sbjct: 181 SAAVDLRPNCGVNRLLIDKLSVRTPTGEVKLKIMKEIAKEHKIEWDTTESEKELLKPSEE 240

Query: 597 LIEGPRTFVSAASLPVKPVARQSAGDNAQFGRTANSRDDGSMHFQDTASAAEAAAKSAKQ 656
           LIEGPRTFVSAASLPVKP+   S  DNAQ  RT NSR++ SMHFQD+ASAAEAAAK+AKQ
Sbjct: 241 LIEGPRTFVSAASLPVKPIVSHS--DNAQIERTTNSRENESMHFQDSASAAEAAAKAAKQ 300

Query: 657 AIAAAQAAAYLANKDLNRDVRGSGFDLGFEQGPPANSTPTNSHNMDHQFRAGEERTTPPQ 716
           AIAAA+AAAYLANKDLNRD   SGF+LGF  GPPANSTPT S+NM+HQF+AGE+ T P Q
Sbjct: 301 AIAAAEAAAYLANKDLNRDAGDSGFNLGFGGGPPANSTPTRSYNMNHQFKAGEDGTAPTQ 360

Query: 717 SFGRCRSLKNDETRHVNTDYEMAYRRHSYNPTPSAHTDIKFDESDCEEEGTQTEEAGGGA 776
           SFGRC SLKN+ETR+VNTDYEMAYRRHSYNP     TDIKFDESDCEEE    +EA  G 
Sbjct: 361 SFGRCSSLKNEETRNVNTDYEMAYRRHSYNP-----TDIKFDESDCEEETEMEDEADRGV 420

Query: 777 YRPPDRNPPPVPSSRVHPKLPDYDTLAARFEALKYRK 809
            RPPDRNPPP PSSRVHPKLPDYDTLAARFEALKYRK
Sbjct: 421 SRPPDRNPPPAPSSRVHPKLPDYDTLAARFEALKYRK 422

BLAST of Sgr017162 vs. NCBI nr
Match: XP_031743525.1 (IST1-like protein isoform X1 [Cucumis sativus])

HSP 1 Score: 643.3 bits (1658), Expect = 2.9e-180
Identity = 360/458 (78.60%), Postives = 381/458 (83.19%), Query Frame = 0

Query: 357 MGAASEAAARSIKIVKLVVSLLRRGFNSSKWFCYFLFLLFLAALTNPFCYRLFSLVLDSK 416
           M AA+EAAARS+KIVK  +++LR GFNSSK                             K
Sbjct: 1   MSAATEAAARSLKIVKQFITILRCGFNSSK----------------------------CK 60

Query: 417 TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEII 476
           TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEII
Sbjct: 61  TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEII 120

Query: 477 ELFCELVVARLSIIAKQRS-----KEGVASLIFAAPRCSEIPELSGLRKVFEKKYGKDFV 536
           ELFCELVVARLSIIAKQR      KEGVASLIFAAPRCSEIPELS LR VFEKKYGKDFV
Sbjct: 121 ELFCELVVARLSIIAKQRQCPADLKEGVASLIFAAPRCSEIPELSALRNVFEKKYGKDFV 180

Query: 537 SAATDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHQIEWDTTESEKELLKPPEE 596
           SAA DLRPNCGVNRLLIDKLSVRTPTG+VKLKIMKEIAKEH+IEWDTTESEKELLKP EE
Sbjct: 181 SAAVDLRPNCGVNRLLIDKLSVRTPTGEVKLKIMKEIAKEHKIEWDTTESEKELLKPSEE 240

Query: 597 LIEGPRTFVSAASLPVKPVARQSAGDNAQF-GRTANSRDDGSMHFQDTASAAEAAAKSAK 656
           LIEGPRTFVSAASLPVKP+   S  DNAQ   RT NSR++ SMHFQD+ASAAEAAAK+AK
Sbjct: 241 LIEGPRTFVSAASLPVKPIVSHS--DNAQIESRTTNSRENESMHFQDSASAAEAAAKAAK 300

Query: 657 QAIAAAQAAAYLANKDLNRDVRGSGFDLGFEQGPPANSTPTNSHNMDHQFRAGEERTTPP 716
           QAIAAA+AAAYLANKDLNRD   SGF+LGF  GPPANSTPT S+NM+HQF+AGE+ T P 
Sbjct: 301 QAIAAAEAAAYLANKDLNRDAGDSGFNLGFGGGPPANSTPTRSYNMNHQFKAGEDGTAPT 360

Query: 717 QSFGRCRSLKNDETRHVNTDYEMAYRRHSYNPTPSAHTDIKFDESDCEEEGTQTEEAGGG 776
           QSFGRC SLKN+ETR+VNTDYEMAYRRHSYNP     TDIKFDESDCEEE    +EA  G
Sbjct: 361 QSFGRCSSLKNEETRNVNTDYEMAYRRHSYNP-----TDIKFDESDCEEETEMEDEADRG 420

Query: 777 AYRPPDRNPPPVPSSRVHPKLPDYDTLAARFEALKYRK 809
             RPPDRNPPP PSSRVHPKLPDYDTLAARFEALKYRK
Sbjct: 421 VSRPPDRNPPPAPSSRVHPKLPDYDTLAARFEALKYRK 423

BLAST of Sgr017162 vs. NCBI nr
Match: XP_008440890.1 (PREDICTED: IST1-like protein [Cucumis melo] >XP_008440891.1 PREDICTED: IST1-like protein [Cucumis melo] >KAA0025625.1 IST1-like protein [Cucumis melo var. makuwa] >TYK12500.1 IST1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 638.6 bits (1646), Expect = 7.0e-179
Identity = 359/458 (78.38%), Postives = 380/458 (82.97%), Query Frame = 0

Query: 357 MGAASEAAARSIKIVKLVVSLLRRGFNSSKWFCYFLFLLFLAALTNPFCYRLFSLVLDSK 416
           M AA+EAAARS+KIVK  +++LR GFNSSK                             K
Sbjct: 1   MSAATEAAARSLKIVKQFITILRCGFNSSK----------------------------CK 60

Query: 417 TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEII 476
           TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANE+I
Sbjct: 61  TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEVI 120

Query: 477 ELFCELVVARLSIIAKQRS-----KEGVASLIFAAPRCSEIPELSGLRKVFEKKYGKDFV 536
           ELFCELVVARLSIIAKQR      KEGVASLIFAAPRCSEIPELS LR VFEKKYGKDFV
Sbjct: 121 ELFCELVVARLSIIAKQRQCPADLKEGVASLIFAAPRCSEIPELSALRNVFEKKYGKDFV 180

Query: 537 SAATDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHQIEWDTTESEKELLKPPEE 596
           SAA DLRPNCGVNRLLIDKLSVRTPTG+VKLKIMKEIAKEH+IEWDTTESEKELLKPPEE
Sbjct: 181 SAAVDLRPNCGVNRLLIDKLSVRTPTGEVKLKIMKEIAKEHKIEWDTTESEKELLKPPEE 240

Query: 597 LIEGPRTFVSAASLPVKPVARQSAGDNAQFGRTANSRDDGSMHFQDTASAAEAAAKSAKQ 656
           LIEGPRTFVSAASLPVKP+   S  DNAQ  RT N  ++GSMHFQDTASAAEAAAK+AKQ
Sbjct: 241 LIEGPRTFVSAASLPVKPIVSHS--DNAQIERTTNC-ENGSMHFQDTASAAEAAAKAAKQ 300

Query: 657 AIAAAQAAAYLANKDLNRDVRGSGFDLGFEQGPPANSTPTNSHNMDHQFRAGEERTTPPQ 716
           AIAAA+AAAYLANKD N+D R SGF+LGF  G PANST   S+NMDHQF+AGE+ T P Q
Sbjct: 301 AIAAAEAAAYLANKDRNQDARDSGFNLGF-GGSPANSTSNRSYNMDHQFKAGEDGTAPTQ 360

Query: 717 SFGRCRSLKNDETRHVNTDYEMAYRRHSYNPTPSAHTDIKFDESDCEEEGTQTEEAGGGA 776
           SFGRC SLKN+ETR+VNTDYEMAYRRHSYNP     TDIKFDESDCEEE    +EAG G 
Sbjct: 361 SFGRCFSLKNEETRNVNTDYEMAYRRHSYNP-----TDIKFDESDCEEETEMDDEAGRGV 420

Query: 777 YRPPDRNPPPVPSSRVHPKLPDYDTLAARFEALKYRKT 810
            RPPDRNPPP PSSRVHPKLPDYDTLAARFEALKYRKT
Sbjct: 421 SRPPDRNPPPAPSSRVHPKLPDYDTLAARFEALKYRKT 421

BLAST of Sgr017162 vs. NCBI nr
Match: XP_022132738.1 (IST1-like protein [Momordica charantia])

HSP 1 Score: 635.6 bits (1638), Expect = 6.0e-178
Identity = 372/501 (74.25%), Postives = 398/501 (79.44%), Query Frame = 0

Query: 317 PFYAPHCPSAFLFQDRFTVPDAALLLPVPLFAASPSRPPAM-GAASEAAARSIKIVKLVV 376
           PF +P        +  F++P A      PLF   P   PAM  AA+EAAARS+KIVKL +
Sbjct: 13  PFISP------TVRSHFSLPRAVSHSLTPLFLFLPPHGPAMTAAATEAAARSLKIVKLFI 72

Query: 377 SLLRRGFNSSKWFCYFLFLLFLAALTNPFCYRLFSLVLDSKTAAKMAVARIKLLRNKREA 436
           +LLRR FNSSK                             KTAAKMAVARIKLLRNKREA
Sbjct: 73  ALLRRDFNSSK----------------------------CKTAAKMAVARIKLLRNKREA 132

Query: 437 VVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRS 496
           VV+QMRRDIALLLQSGQDATARIRVEHVIREQNVLA NEIIELFCELVVARLSIIAKQR 
Sbjct: 133 VVRQMRRDIALLLQSGQDATARIRVEHVIREQNVLAGNEIIELFCELVVARLSIIAKQRE 192

Query: 497 -----KEGVASLIFAAPRCSEIPELSGLRKVFEKKYGKDFVSAATDLRPNCGVNRLLIDK 556
                KEGVASLIFAAPRCSEIPELS LR VFEKKYGKDFVSAATDLRPNCGVNRLLIDK
Sbjct: 193 CPPDLKEGVASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAATDLRPNCGVNRLLIDK 252

Query: 557 LSVRTPTGQVKLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSAASLPVKPV 616
           LS+RTP+GQVKLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSAASLPVKP+
Sbjct: 253 LSIRTPSGQVKLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSAASLPVKPM 312

Query: 617 ARQSAGDNAQFGRTANSRDDGSMHFQDTASAAEAAAKSAKQAIAAAQAAAYLANKDLNRD 676
           A QSA D AQ GRT NSR+D + HFQDTASAAEAAAK+AKQAIAAAQAAAYLANKD NR 
Sbjct: 313 ANQSARDIAQIGRTTNSREDDT-HFQDTASAAEAAAKAAKQAIAAAQAAAYLANKDSNRG 372

Query: 677 VRGS-GFDLGFEQGPPANSTPTNSHNMD-HQFRAGEERTTPPQSFGRCRSLK-NDETRHV 736
            RG  GF L FE G P NS+PTNSHNMD HQF+AGEE T PPQS GRC SLK N+ETR+V
Sbjct: 373 GRGGLGFGLEFE-GLPTNSSPTNSHNMDNHQFKAGEETTIPPQSLGRCSSLKNNEETRNV 432

Query: 737 NTDYEMAYRRHSYNPTPSAHTDIKFDESDCEEEGTQTEEAGGGAYRPPDRNPPPVPSSRV 796
           NT+Y+ AYRR+SYNP     TDIKFDESDCEEE    +E+ G   +PPDRNPPPVPSSRV
Sbjct: 433 NTNYDEAYRRYSYNP-----TDIKFDESDCEEETQMDDESRGATNQPPDRNPPPVPSSRV 472

Query: 797 HPKLPDYDTLAARFEALKYRK 809
           HPKLPDYDTLAARFEALKY+K
Sbjct: 493 HPKLPDYDTLAARFEALKYKK 472

BLAST of Sgr017162 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 2.7e-16
Identity = 58/164 (35.37%), Postives = 96/164 (58.54%), Query Frame = 0

Query: 420 KMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEIIELF 479
           ++ + R+KLL  K+  + ++ R++IA  L +G+D  ARIRVEH+IRE  ++ A EI+EL+
Sbjct: 16  RLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELY 75

Query: 480 CELVVARLSIIAKQRS-----KEGVASLIFAAPRC-SEIPELSGLRKVFEKKYGKDFVSA 539
           C+L++AR  +I   +       E V++LI+AAPR  SE+ EL  +      KY K++   
Sbjct: 76  CDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKL 135

Query: 540 ATDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHQIEWD 578
               +    VN  L+ KLSV  P   +  + + EIAK + + ++
Sbjct: 136 CRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYE 178

BLAST of Sgr017162 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 2.7e-16
Identity = 58/164 (35.37%), Postives = 96/164 (58.54%), Query Frame = 0

Query: 420 KMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEIIELF 479
           ++ + R+KLL  K+  + ++ R++IA  L +G+D  ARIRVEH+IRE  ++ A EI+EL+
Sbjct: 16  RLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELY 75

Query: 480 CELVVARLSIIAKQRS-----KEGVASLIFAAPRC-SEIPELSGLRKVFEKKYGKDFVSA 539
           C+L++AR  +I   +       E V++LI+AAPR  SE+ EL  +      KY K++   
Sbjct: 76  CDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKL 135

Query: 540 ATDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHQIEWD 578
               +    VN  L+ KLSV  P   +  + + EIAK + + ++
Sbjct: 136 CRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYE 178

BLAST of Sgr017162 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 2.7e-16
Identity = 58/164 (35.37%), Postives = 96/164 (58.54%), Query Frame = 0

Query: 420 KMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEIIELF 479
           ++ + R+KLL  K+  + ++ R++IA  L +G+D  ARIRVEH+IRE  ++ A EI+EL+
Sbjct: 16  RLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELY 75

Query: 480 CELVVARLSIIAKQRS-----KEGVASLIFAAPRC-SEIPELSGLRKVFEKKYGKDFVSA 539
           C+L++AR  +I   +       E V++LI+AAPR  SE+ EL  +      KY K++   
Sbjct: 76  CDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKL 135

Query: 540 ATDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHQIEWD 578
               +    VN  L+ KLSV  P   +  + + EIAK + + ++
Sbjct: 136 CRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYE 178

BLAST of Sgr017162 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 2.7e-16
Identity = 58/164 (35.37%), Postives = 96/164 (58.54%), Query Frame = 0

Query: 420 KMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEIIELF 479
           ++ + R+KLL  K+  + ++ R++IA  L +G+D  ARIRVEH+IRE  ++ A EI+EL+
Sbjct: 16  RLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELY 75

Query: 480 CELVVARLSIIAKQRS-----KEGVASLIFAAPRC-SEIPELSGLRKVFEKKYGKDFVSA 539
           C+L++AR  +I   +       E V++LI+AAPR  SE+ EL  +      KY K++   
Sbjct: 76  CDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKL 135

Query: 540 ATDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHQIEWD 578
               +    VN  L+ KLSV  P   +  + + EIAK + + ++
Sbjct: 136 CRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYE 178

BLAST of Sgr017162 vs. ExPASy Swiss-Prot
Match: Q5R6G8 (IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 6.1e-16
Identity = 58/164 (35.37%), Postives = 95/164 (57.93%), Query Frame = 0

Query: 420 KMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEIIELF 479
           ++ + R+KLL  K+  + ++ R++IA  L +G+D  ARIRVEH+IRE  ++ A EI+EL+
Sbjct: 16  RLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELY 75

Query: 480 CELVVARLSIIAKQRS-----KEGVASLIFAAPRC-SEIPELSGLRKVFEKKYGKDFVSA 539
           C+L++AR  +I   +       E V++LI+AAPR  SE+ EL  +      KY K +   
Sbjct: 76  CDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKGYGKL 135

Query: 540 ATDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHQIEWD 578
               +    VN  L+ KLSV  P   +  + + EIAK + + ++
Sbjct: 136 CRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYE 178

BLAST of Sgr017162 vs. ExPASy TrEMBL
Match: A0A0A0KKW3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G507450 PE=3 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 9.6e-182
Identity = 360/457 (78.77%), Postives = 381/457 (83.37%), Query Frame = 0

Query: 357 MGAASEAAARSIKIVKLVVSLLRRGFNSSKWFCYFLFLLFLAALTNPFCYRLFSLVLDSK 416
           M AA+EAAARS+KIVK  +++LR GFNSSK                             K
Sbjct: 1   MSAATEAAARSLKIVKQFITILRCGFNSSK----------------------------CK 60

Query: 417 TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEII 476
           TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEII
Sbjct: 61  TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEII 120

Query: 477 ELFCELVVARLSIIAKQRS-----KEGVASLIFAAPRCSEIPELSGLRKVFEKKYGKDFV 536
           ELFCELVVARLSIIAKQR      KEGVASLIFAAPRCSEIPELS LR VFEKKYGKDFV
Sbjct: 121 ELFCELVVARLSIIAKQRQCPADLKEGVASLIFAAPRCSEIPELSALRNVFEKKYGKDFV 180

Query: 537 SAATDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHQIEWDTTESEKELLKPPEE 596
           SAA DLRPNCGVNRLLIDKLSVRTPTG+VKLKIMKEIAKEH+IEWDTTESEKELLKP EE
Sbjct: 181 SAAVDLRPNCGVNRLLIDKLSVRTPTGEVKLKIMKEIAKEHKIEWDTTESEKELLKPSEE 240

Query: 597 LIEGPRTFVSAASLPVKPVARQSAGDNAQFGRTANSRDDGSMHFQDTASAAEAAAKSAKQ 656
           LIEGPRTFVSAASLPVKP+   S  DNAQ  RT NSR++ SMHFQD+ASAAEAAAK+AKQ
Sbjct: 241 LIEGPRTFVSAASLPVKPIVSHS--DNAQIERTTNSRENESMHFQDSASAAEAAAKAAKQ 300

Query: 657 AIAAAQAAAYLANKDLNRDVRGSGFDLGFEQGPPANSTPTNSHNMDHQFRAGEERTTPPQ 716
           AIAAA+AAAYLANKDLNRD   SGF+LGF  GPPANSTPT S+NM+HQF+AGE+ T P Q
Sbjct: 301 AIAAAEAAAYLANKDLNRDAGDSGFNLGFGGGPPANSTPTRSYNMNHQFKAGEDGTAPTQ 360

Query: 717 SFGRCRSLKNDETRHVNTDYEMAYRRHSYNPTPSAHTDIKFDESDCEEEGTQTEEAGGGA 776
           SFGRC SLKN+ETR+VNTDYEMAYRRHSYNP     TDIKFDESDCEEE    +EA  G 
Sbjct: 361 SFGRCSSLKNEETRNVNTDYEMAYRRHSYNP-----TDIKFDESDCEEETEMEDEADRGV 420

Query: 777 YRPPDRNPPPVPSSRVHPKLPDYDTLAARFEALKYRK 809
            RPPDRNPPP PSSRVHPKLPDYDTLAARFEALKYRK
Sbjct: 421 SRPPDRNPPPAPSSRVHPKLPDYDTLAARFEALKYRK 422

BLAST of Sgr017162 vs. ExPASy TrEMBL
Match: A0A2N9I1V1 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS45906 PE=3 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 6.2e-181
Identity = 432/852 (50.70%), Postives = 516/852 (60.56%), Query Frame = 0

Query: 1   MVAFSQTPWSRYRDRCISLPARSHPSTIRVEQELAKLRSSDSSSFSSIEAICGSLSGLVE 60
           + AFS    + Y  R ISLP RSHPSTIRVE+EL KL+S ++SS S +E IC  LSGL E
Sbjct: 7   IAAFSSKASNGYSVRSISLPVRSHPSTIRVEEELNKLKSWEASSSSKVETICIGLSGLSE 66

Query: 61  LYRCINEVLNLPLTQQALASHRRENWVQELVDGSVRFLDICDNTRDVVLVMKESIRQLQS 120
           LY                                          RD +L+M+E+IR+LQS
Sbjct: 67  LY------------------------------------------RDAILLMQETIRELQS 126

Query: 121 AIRRSKYEDSGVENSIAAYT-------------------MDNTAGAFPPLQLENEVTAAV 180
           ++RR K  D  +E+ + AY                    MDN  GAFP L L++ + +AV
Sbjct: 127 SLRRIKVGDLSIESIVTAYNCSRRKMRKEIARSLASLKPMDNEIGAFPMLNLDHHL-SAV 186

Query: 181 IRVLREASAVTTSIFNSLLSFLTVPVHWRPKPSRWTLVSRLVQKGAITRNNQAERINEFE 240
           +RVL E S +T+SIF SLL FL+VPV  +PKPSRW+LVS+LVQKGA+ R +Q E +NE E
Sbjct: 187 VRVLNETSLMTSSIFQSLLLFLSVPV-LKPKPSRWSLVSKLVQKGALARKDQPENMNELE 246

Query: 241 NLDTALDGLQFGSLSRDAEVKEKIQSVVRERLQLPLPRVTLSPPLQYGRIGFFLSHPTPQ 300
            +D AL  L   + S+D E ++KIQS      Q+ L        L  G +G        +
Sbjct: 247 GVDIALSSLLMHNSSKDIE-EQKIQSA-----QIKL------DALDVGMVGL-------E 306

Query: 301 NLLQRLYQFFLQFNGRRGSGAASVIVLMIYSSSRSPFYAPHCPSAFLFQDRFTVPDAALL 360
             L+ L++  +Q           V  L I S         H      +  R T       
Sbjct: 307 KCLECLFRHLIQ---------TRVFYLNIISHFAYTNTIEHS-----YTHRLT------- 366

Query: 361 LPVPLFAASPSRPPAMGAASEAAARSIKIVKLVVSLLRRGFNSSKWFCYFLFLLFLAALT 420
                     +RP  M AAS A + +  I+K+ +SL RRGFNSSK               
Sbjct: 367 ----------TRP--MTAASVATSHAKNIMKIGLSLFRRGFNSSK--------------- 426

Query: 421 NPFCYRLFSLVLDSKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVE 480
                         KTAAKMAVARIKLLRNKRE VVKQMRRDIALLLQSGQDATA+IRVE
Sbjct: 427 -------------CKTAAKMAVARIKLLRNKREVVVKQMRRDIALLLQSGQDATAQIRVE 486

Query: 481 HVIREQNVLAANEIIELFCELVVARLSIIAKQRS-----KEGVASLIFAAPRCSEIPELS 540
           HVIREQNVLAANE IELFCELVVARLSII++QR      KEG+AS+IFA+PRCSEIPEL 
Sbjct: 487 HVIREQNVLAANEFIELFCELVVARLSIISQQRECPADLKEGIASIIFASPRCSEIPELV 546

Query: 541 GLRKVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHQIEW 600
            LR +FEKKYGKDFVSAATDLRPNCGVNR+LIDKLSVRTP G+VKLKIMKEIAKEHQI+W
Sbjct: 547 ALRNIFEKKYGKDFVSAATDLRPNCGVNRMLIDKLSVRTPAGEVKLKIMKEIAKEHQIQW 606

Query: 601 DTTESEKELLKPPEELIEGPRTFVSAASLPVKPVARQSAGDNAQFGRTANSRDDGSMHFQ 660
           DTTESE ELLKPPEE IEGPR+FVSA SLP+KP   QSA  N    R+ ++R++G+MHF+
Sbjct: 607 DTTESEMELLKPPEERIEGPRSFVSATSLPMKPQPTQSAEPNKPTTRSTSNRENGNMHFE 666

Query: 661 DTASAAEAAAKSAKQAIAAAQAAAYLANKDLNRDVRGSGFD--LGFEQGPPANSTPTNSH 720
           DTASAAEAAA+SAKQAIAAAQAAAYLANK+ N+    S  D  L      P + +P NS 
Sbjct: 667 DTASAAEAAAESAKQAIAAAQAAAYLANKNFNQTTEASSLDDKLNDSWVMPNHHSPVNSQ 726

Query: 721 NMDHQFRAGEERTTPPQSFGRCRSLKNDE---TRHVNT-------DYEMAYRRHSYNPTP 780
           NM HQ +A   RT   QSF       ++E   T +VN+        Y     RHSYN  P
Sbjct: 727 NMGHQQKA-HGRTYASQSFRTTPFGSSEEETPTSNVNSGKVYRRHSYNAPPSRHSYNAPP 732

Query: 781 SAHTDIKFDESDCEEEGTQTEEAGGGAYRPPDRNPPPVPSSR--------VHPKLPDYDT 809
           S H+DIKFDESDC+EE  + EE   G + PP R PPP PS +        VHPKLPDYD 
Sbjct: 787 SEHSDIKFDESDCDEE-IEMEEPPSGIHPPPKRPPPPAPSFQDKQDSNLHVHPKLPDYDA 732

BLAST of Sgr017162 vs. ExPASy TrEMBL
Match: A0A1S3B2X8 (IST1-like protein OS=Cucumis melo OX=3656 GN=LOC103485176 PE=3 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 3.4e-179
Identity = 359/458 (78.38%), Postives = 380/458 (82.97%), Query Frame = 0

Query: 357 MGAASEAAARSIKIVKLVVSLLRRGFNSSKWFCYFLFLLFLAALTNPFCYRLFSLVLDSK 416
           M AA+EAAARS+KIVK  +++LR GFNSSK                             K
Sbjct: 1   MSAATEAAARSLKIVKQFITILRCGFNSSK----------------------------CK 60

Query: 417 TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEII 476
           TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANE+I
Sbjct: 61  TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEVI 120

Query: 477 ELFCELVVARLSIIAKQRS-----KEGVASLIFAAPRCSEIPELSGLRKVFEKKYGKDFV 536
           ELFCELVVARLSIIAKQR      KEGVASLIFAAPRCSEIPELS LR VFEKKYGKDFV
Sbjct: 121 ELFCELVVARLSIIAKQRQCPADLKEGVASLIFAAPRCSEIPELSALRNVFEKKYGKDFV 180

Query: 537 SAATDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHQIEWDTTESEKELLKPPEE 596
           SAA DLRPNCGVNRLLIDKLSVRTPTG+VKLKIMKEIAKEH+IEWDTTESEKELLKPPEE
Sbjct: 181 SAAVDLRPNCGVNRLLIDKLSVRTPTGEVKLKIMKEIAKEHKIEWDTTESEKELLKPPEE 240

Query: 597 LIEGPRTFVSAASLPVKPVARQSAGDNAQFGRTANSRDDGSMHFQDTASAAEAAAKSAKQ 656
           LIEGPRTFVSAASLPVKP+   S  DNAQ  RT N  ++GSMHFQDTASAAEAAAK+AKQ
Sbjct: 241 LIEGPRTFVSAASLPVKPIVSHS--DNAQIERTTNC-ENGSMHFQDTASAAEAAAKAAKQ 300

Query: 657 AIAAAQAAAYLANKDLNRDVRGSGFDLGFEQGPPANSTPTNSHNMDHQFRAGEERTTPPQ 716
           AIAAA+AAAYLANKD N+D R SGF+LGF  G PANST   S+NMDHQF+AGE+ T P Q
Sbjct: 301 AIAAAEAAAYLANKDRNQDARDSGFNLGF-GGSPANSTSNRSYNMDHQFKAGEDGTAPTQ 360

Query: 717 SFGRCRSLKNDETRHVNTDYEMAYRRHSYNPTPSAHTDIKFDESDCEEEGTQTEEAGGGA 776
           SFGRC SLKN+ETR+VNTDYEMAYRRHSYNP     TDIKFDESDCEEE    +EAG G 
Sbjct: 361 SFGRCFSLKNEETRNVNTDYEMAYRRHSYNP-----TDIKFDESDCEEETEMDDEAGRGV 420

Query: 777 YRPPDRNPPPVPSSRVHPKLPDYDTLAARFEALKYRKT 810
            RPPDRNPPP PSSRVHPKLPDYDTLAARFEALKYRKT
Sbjct: 421 SRPPDRNPPPAPSSRVHPKLPDYDTLAARFEALKYRKT 421

BLAST of Sgr017162 vs. ExPASy TrEMBL
Match: A0A5A7SMF5 (IST1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00780 PE=3 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 3.4e-179
Identity = 359/458 (78.38%), Postives = 380/458 (82.97%), Query Frame = 0

Query: 357 MGAASEAAARSIKIVKLVVSLLRRGFNSSKWFCYFLFLLFLAALTNPFCYRLFSLVLDSK 416
           M AA+EAAARS+KIVK  +++LR GFNSSK                             K
Sbjct: 1   MSAATEAAARSLKIVKQFITILRCGFNSSK----------------------------CK 60

Query: 417 TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEII 476
           TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANE+I
Sbjct: 61  TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEVI 120

Query: 477 ELFCELVVARLSIIAKQRS-----KEGVASLIFAAPRCSEIPELSGLRKVFEKKYGKDFV 536
           ELFCELVVARLSIIAKQR      KEGVASLIFAAPRCSEIPELS LR VFEKKYGKDFV
Sbjct: 121 ELFCELVVARLSIIAKQRQCPADLKEGVASLIFAAPRCSEIPELSALRNVFEKKYGKDFV 180

Query: 537 SAATDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHQIEWDTTESEKELLKPPEE 596
           SAA DLRPNCGVNRLLIDKLSVRTPTG+VKLKIMKEIAKEH+IEWDTTESEKELLKPPEE
Sbjct: 181 SAAVDLRPNCGVNRLLIDKLSVRTPTGEVKLKIMKEIAKEHKIEWDTTESEKELLKPPEE 240

Query: 597 LIEGPRTFVSAASLPVKPVARQSAGDNAQFGRTANSRDDGSMHFQDTASAAEAAAKSAKQ 656
           LIEGPRTFVSAASLPVKP+   S  DNAQ  RT N  ++GSMHFQDTASAAEAAAK+AKQ
Sbjct: 241 LIEGPRTFVSAASLPVKPIVSHS--DNAQIERTTNC-ENGSMHFQDTASAAEAAAKAAKQ 300

Query: 657 AIAAAQAAAYLANKDLNRDVRGSGFDLGFEQGPPANSTPTNSHNMDHQFRAGEERTTPPQ 716
           AIAAA+AAAYLANKD N+D R SGF+LGF  G PANST   S+NMDHQF+AGE+ T P Q
Sbjct: 301 AIAAAEAAAYLANKDRNQDARDSGFNLGF-GGSPANSTSNRSYNMDHQFKAGEDGTAPTQ 360

Query: 717 SFGRCRSLKNDETRHVNTDYEMAYRRHSYNPTPSAHTDIKFDESDCEEEGTQTEEAGGGA 776
           SFGRC SLKN+ETR+VNTDYEMAYRRHSYNP     TDIKFDESDCEEE    +EAG G 
Sbjct: 361 SFGRCFSLKNEETRNVNTDYEMAYRRHSYNP-----TDIKFDESDCEEETEMDDEAGRGV 420

Query: 777 YRPPDRNPPPVPSSRVHPKLPDYDTLAARFEALKYRKT 810
            RPPDRNPPP PSSRVHPKLPDYDTLAARFEALKYRKT
Sbjct: 421 SRPPDRNPPPAPSSRVHPKLPDYDTLAARFEALKYRKT 421

BLAST of Sgr017162 vs. ExPASy TrEMBL
Match: A0A6J1BX61 (IST1-like protein OS=Momordica charantia OX=3673 GN=LOC111005530 PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 2.9e-178
Identity = 372/501 (74.25%), Postives = 398/501 (79.44%), Query Frame = 0

Query: 317 PFYAPHCPSAFLFQDRFTVPDAALLLPVPLFAASPSRPPAM-GAASEAAARSIKIVKLVV 376
           PF +P        +  F++P A      PLF   P   PAM  AA+EAAARS+KIVKL +
Sbjct: 13  PFISP------TVRSHFSLPRAVSHSLTPLFLFLPPHGPAMTAAATEAAARSLKIVKLFI 72

Query: 377 SLLRRGFNSSKWFCYFLFLLFLAALTNPFCYRLFSLVLDSKTAAKMAVARIKLLRNKREA 436
           +LLRR FNSSK                             KTAAKMAVARIKLLRNKREA
Sbjct: 73  ALLRRDFNSSK----------------------------CKTAAKMAVARIKLLRNKREA 132

Query: 437 VVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRS 496
           VV+QMRRDIALLLQSGQDATARIRVEHVIREQNVLA NEIIELFCELVVARLSIIAKQR 
Sbjct: 133 VVRQMRRDIALLLQSGQDATARIRVEHVIREQNVLAGNEIIELFCELVVARLSIIAKQRE 192

Query: 497 -----KEGVASLIFAAPRCSEIPELSGLRKVFEKKYGKDFVSAATDLRPNCGVNRLLIDK 556
                KEGVASLIFAAPRCSEIPELS LR VFEKKYGKDFVSAATDLRPNCGVNRLLIDK
Sbjct: 193 CPPDLKEGVASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAATDLRPNCGVNRLLIDK 252

Query: 557 LSVRTPTGQVKLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSAASLPVKPV 616
           LS+RTP+GQVKLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSAASLPVKP+
Sbjct: 253 LSIRTPSGQVKLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSAASLPVKPM 312

Query: 617 ARQSAGDNAQFGRTANSRDDGSMHFQDTASAAEAAAKSAKQAIAAAQAAAYLANKDLNRD 676
           A QSA D AQ GRT NSR+D + HFQDTASAAEAAAK+AKQAIAAAQAAAYLANKD NR 
Sbjct: 313 ANQSARDIAQIGRTTNSREDDT-HFQDTASAAEAAAKAAKQAIAAAQAAAYLANKDSNRG 372

Query: 677 VRGS-GFDLGFEQGPPANSTPTNSHNMD-HQFRAGEERTTPPQSFGRCRSLK-NDETRHV 736
            RG  GF L FE G P NS+PTNSHNMD HQF+AGEE T PPQS GRC SLK N+ETR+V
Sbjct: 373 GRGGLGFGLEFE-GLPTNSSPTNSHNMDNHQFKAGEETTIPPQSLGRCSSLKNNEETRNV 432

Query: 737 NTDYEMAYRRHSYNPTPSAHTDIKFDESDCEEEGTQTEEAGGGAYRPPDRNPPPVPSSRV 796
           NT+Y+ AYRR+SYNP     TDIKFDESDCEEE    +E+ G   +PPDRNPPPVPSSRV
Sbjct: 433 NTNYDEAYRRYSYNP-----TDIKFDESDCEEETQMDDESRGATNQPPDRNPPPVPSSRV 472

Query: 797 HPKLPDYDTLAARFEALKYRK 809
           HPKLPDYDTLAARFEALKY+K
Sbjct: 493 HPKLPDYDTLAARFEALKYKK 472

BLAST of Sgr017162 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 398.3 bits (1022), Expect = 1.5e-110
Identity = 258/493 (52.33%), Postives = 309/493 (62.68%), Query Frame = 0

Query: 357 MGAASEAAARSIKIVKLVVSLLRRGFNSSKWFCYFLFLLFLAALTNPFCYRLFSLVLDSK 416
           M AA  A+A++ K++K  +SL RRGFNSSK                             K
Sbjct: 1   MTAAEVASAQTKKLMKFSLSLFRRGFNSSK----------------------------CK 60

Query: 417 TAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEII 476
           TAAKMAVARIKL+RNKR  VVKQMRRDIA+LLQSGQDATARIRVEHVIREQN+ AANEII
Sbjct: 61  TAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQAANEII 120

Query: 477 ELFCELVVARLSIIAKQRS-----KEGVASLIFAAPRCSEIPELSGLRKVFEKKYGKDFV 536
           ELFCEL+V+RL+II KQ+      KEG+ASLIFAAPRCSEIPEL  LR +F KKYGKDFV
Sbjct: 121 ELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKYGKDFV 180

Query: 537 SAATDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHQIEWDTTESEKELLKPPEE 596
           SAATDLRP+CGVNR+LIDKLSVR P G+ KLKIMKEIAKE Q++WDTTE+E+ELLKP EE
Sbjct: 181 SAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETEQELLKPQEE 240

Query: 597 LIEGPRTFVSAASLPVKPVARQSAGDNAQFGRTANSRDDGSMHFQDTASAAEAAAKSAKQ 656
            I+GPR FVSA+SLPV   A     D  +    + S    + H+ DT SAAEAA + AKQ
Sbjct: 241 SIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTHYHDTESAAEAATELAKQ 300

Query: 657 AIAAAQAAAYLANKDLNRDVRGSGFDLGFEQGPPANSTPTNSHNMDHQFRAGEERTTPPQ 716
           A+AAAQ A+ LA +   RD     F +  +       +    H+  H     + R +   
Sbjct: 301 AVAAAQVASLLATR---RDSSNKEFSVSSDHSTHQKDSQYMDHHHHHPGSRRQSRDSETS 360

Query: 717 SF-----------GRCRSLKND-------ETRHVNTD---YEMAYRRHSYNP---TPSAH 776
           S+           GR  S  N        E  + NT+    E   RRHSYNP    P A 
Sbjct: 361 SYYAKPGAENRGMGRRHSYNNPGINESDYEEEYTNTEAEAKETMRRRHSYNPRSVPPPAT 420

Query: 777 TDIKFDESDCEEEGTQTEEAGGG--AYRPPDRNPPPVPSS----------RVHPKLPDYD 809
           ++IKFDESD  EE T+ +E   G  +  PP+R PP  P S          +VHPKLPDYD
Sbjct: 421 SEIKFDESDYYEEETEPDEPSQGRVSSLPPNRAPPQAPQSGESRQDSSGHQVHPKLPDYD 462

BLAST of Sgr017162 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 204.1 bits (518), Expect = 4.1e-52
Identity = 117/273 (42.86%), Postives = 174/273 (63.74%), Query Frame = 0

Query: 416 KTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEI 475
           KT  K+ + RIKL+RN+REA +KQMRR+IA LL++GQ+ATARIRVEH+IRE+ ++AA EI
Sbjct: 18  KTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIREEKMMAAQEI 77

Query: 476 IELFCELVVARLSIIAKQRS-----KEGVASLIFAAPRCSEIPELSGLRKVFEKKYGKDF 535
           +ELFCEL+  RL II  QR      KE ++S+ FAAPRCS++ EL  ++ +F  KYGK+F
Sbjct: 78  LELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQILFVSKYGKEF 137

Query: 536 VSAATDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHQIEWDTTESEKELLKPPE 595
           V+AA++L+P+ GVNR L++ LSVR P+ + KLK++KEIA+EH+++WD   +E +L K  E
Sbjct: 138 VAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEEHELDWDPASTETDLFKSHE 197

Query: 596 ELIEGPRTFVSAASLPVKPVARQSAGDNAQFGRTANSRDDGSMHFQDTASAAEAAAKSAK 655
           +L++GP+ F   + LP+     +     +       S  D      D         +   
Sbjct: 198 DLLDGPKQFGGGSKLPLPEEQNEKTNLTSLSAAKEKSDSDSEYDILDFPEVPNVLLRPTP 257

Query: 656 QAIA-----AAQAAAY-LANKDLNRDVRGSGFD 678
            A +     AA++A+Y   + DL  D   +G +
Sbjct: 258 GATSVNAPDAAKSASYEHTSHDLPFDSENAGVE 290

BLAST of Sgr017162 vs. TAIR 10
Match: AT1G34220.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 188.3 bits (477), Expect = 2.3e-47
Identity = 117/303 (38.61%), Postives = 174/303 (57.43%), Query Frame = 0

Query: 416 KTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEI 475
           KT  K+ + RIKL+RN+REA +KQMRR+IA LL++GQ+ATARIRVEH+IRE+ ++AA EI
Sbjct: 18  KTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIREEKMMAAQEI 77

Query: 476 IELFCELVVARLSIIAKQRS-----KEGVASLIFAAPRCSEIPELSGLRKVFEKKYGKDF 535
           +ELFCEL+  RL II  QR      KE ++S+ FAAPRCS++ EL  ++ +F  KYGK+F
Sbjct: 78  LELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQILFVSKYGKEF 137

Query: 536 VSAATDLRPNCGVNR------------------------------LLIDKLSVRTPTGQV 595
           V+AA++L+P+ GVNR                               L++ LSVR P+ + 
Sbjct: 138 VAAASELKPDSGVNRKTESLIFIAWFSLVETRDLFMFLYFSNSILQLVELLSVRAPSPET 197

Query: 596 KLKIMKEIAKEHQIEWDTTESEKELLKPPEELIEGPRTFVSAASLPVKPVARQSAGDNAQ 655
           KLK++KEIA+EH+++WD   +E +L K  E+L++GP+ F   + LP+     +     + 
Sbjct: 198 KLKLLKEIAEEHELDWDPASTETDLFKSHEDLLDGPKQFGGGSKLPLPEEQNEKTNLTSL 257

Query: 656 FGRTANSRDDGSMHFQDTASAAEAAAKSAKQAIA-----AAQAAAY-LANKDLNRDVRGS 678
                 S  D      D         +    A +     AA++A+Y   + DL  D   +
Sbjct: 258 SAAKEKSDSDSEYDILDFPEVPNVLLRPTPGATSVNAPDAAKSASYEHTSHDLPFDSENA 317

BLAST of Sgr017162 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 186.4 bits (472), Expect = 8.9e-47
Identity = 111/286 (38.81%), Postives = 171/286 (59.79%), Query Frame = 0

Query: 416 KTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEI 475
           KT+  +A+AR+KLL+NKR+  +K M+++IA  LQ+GQ+  ARIRVEHVIRE N+ AA EI
Sbjct: 18  KTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVEHVIREMNLWAAYEI 77

Query: 476 IELFCELVVARLSIIAKQRS-----KEGVASLIFAAPRCSEIPELSGLRKVFEKKYGKDF 535
           +ELFCE ++AR+ I+  ++      +E +AS+IFAAPRCSE+P+L  ++ +F  KYGK+F
Sbjct: 78  LELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLLQIKNLFGTKYGKEF 137

Query: 536 VSAATDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHQIEWDTTESEKELLKPPE 595
           +  A++LRP+ GVNR +I+KLS  +P+G  +LK++KEIA+E+ + WD++ +E E +K  E
Sbjct: 138 IMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSLNWDSSATEAEFMKSHE 197

Query: 596 ELIEGPRTFVSAASLPVKPVARQSAGDNAQFGRTANSRDDGSMHFQ-------------- 655
           +L+ G +       +     ++Q  G ++      +   + +  FQ              
Sbjct: 198 DLLGGAKQIHRQDGISESRPSQQGYGQSSVSREVESLPAEATQRFQKLQAQNPVSKSMPS 257

Query: 656 ------------------DTASAAEAAAKSAKQAIAAAQAAAYLAN 665
                             D    A AA  SA +A AAA+AAA L N
Sbjct: 258 SKLTSAFQAPPDTRRNQTDVMEIARAALASADRATAAARAAAQLVN 303

BLAST of Sgr017162 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 166.8 bits (421), Expect = 7.3e-41
Identity = 104/259 (40.15%), Postives = 160/259 (61.78%), Query Frame = 0

Query: 416 KTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEI 475
           KTA +MA +R+K+L+NK+E  +KQ+RR++A LL+SGQ  TARIRVEHV+RE+  +AA E+
Sbjct: 15  KTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVVREEKTVAAYEL 74

Query: 476 IELFCELVVARLSIIAKQRS-----KEGVASLIFAAPRCSEIPELSGLRKVFEKKYGKDF 535
           I ++CEL+V RL +I  Q++     KE V S++FA+ R S++PELS + K F  KYGKDF
Sbjct: 75  IGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIFKQFTTKYGKDF 134

Query: 536 VSAATDLRPNCGVNRLLIDKLSVRTPTGQVKLKIMKEIAKEHQIEWDTTESEKELLKPPE 595
            ++A +LRP+ GV+RLL++KLS + P G  K+KI+  IA+EH + W+  +S  E      
Sbjct: 135 STSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVVWE-AQSFVESDPKDT 194

Query: 596 ELIEGPRTFVSAASLPVKPV--ARQSAGDNAQFGRTANSRDDGS--MHFQDTASAAEAAA 655
           EL+ G  +F  A+S+ +     + +    N     T N+    S   H  + + A    +
Sbjct: 195 ELLNGANSFQPASSMNMDSSINSNKEQPPNIHAPATVNAHHGSSERHHSPENSYANGGRS 254

Query: 656 KSAKQAIAAAQAAAYLANK 666
            S    + + +A  Y  +K
Sbjct: 255 SSRSNNVTSGKADDYYHSK 272