Sgr016966 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr016966
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionDNA-directed RNA polymerase II subunit RPB7
Locationtig00153016: 1090255 .. 1093706 (+)
RNA-Seq ExpressionSgr016966
SyntenySgr016966
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGCCTCTCAATAACCATTGTAGATCAAAATCTGGCATGTTTGATAATACCGGATATAAATTCAAAATATTTATTTTTTATATATCTAATGATATAAAACTACTAAAATTTCTCCAACTCCTACGTATTCCTTCCTCGGCACCCCACCCGCCCCCAAAAAAAAAAAAAAAATTTCTCCTACACGTTCCTCCTGCTCTCCCTCCCTCTTCGGCTTCTGCCGTCCCTCCCCGAAGCCCCTTCCATCGCGCTCAGTTCACCGTCTCCTACTTTCTTGCGTCGCCCCTACGATTTTTCTCAGGTACTCCTCTCAATCCCTTTCTTCTTCTCTGATACGCGTCTGAGAGATTATTATGTTATGCTCCCAAACTTATGGAACCGAATCGAAAAAATCGAACCAACTGGTTCGGTTGAAAATCGAGTTTGTTTAATATTGGACATTGATTCGGTTGTTCAATATCCCAGACCCAAAACATCGGTTCGGTCTCCCACGATCCCAAATTGAGTTTTTGCCTCCCCGTTTGGCCTCACCCAATCAGTCACACAAAACTTTACCAAAATTTGTATTTCATTTTGGCATATTTCAATTTTCTTTGCGCAAATTATACTAGAGGGCCAAAAGGGAATGGAACCTCGAGAAAACCCTACGCTGCAACGAGTGTCTGAACTCTGTCCGGCCAATTCCCTTCTGCTGGCTTACATATCAGGCTAATCATTTTTTTTTAATCTCAGACTCTTGATTCTCTCTTTCATTTTTCCCTGTTCTTCTGCGGTTTGTATCTTCTCTGATTTTTCGATTAAATGTTTCTTTGTTAATTTTCATTTCCTAGAATCTCAGTTGTTACCCTTTTTTCGGGTTGTTTATCCAAATTCTTTCGCCGTAGAAGTTGCATTCATCAATGAGCTCAAATGGGTCTTTCCGCAGGTTGAGAGAGCCACCATGTTCTTCCACATAGTGTTGGAAAGGAATATGCAGCTGCATCCACGGCACTTTGGTCGTAACCTCCGTGAGAATTTGGTTACCAAACTCATGAAAGATGTCGAGGGCACTTGCAGGTTTGTTAACTTGCCTCCCTTTTTATTTGCTGGAACATTACTTTTAACTTCCCAAAAAGCTTATTTTCCCAATGTCCAGATGGATCATGAATACCCGAAAGCCATTGAGAGAGAGTGTCGAAAATGCAAAACCAAATAGATGAAGAGAAGGATCATAAATACCTGCAAGGCATTGAGAGAGAGTGTCCAAAATGCAAAACCAAATGGATAAAGAGAAGGAACCATGGATCACTTTTATAGAGAGTCCTACGAAATAAAAATACCACTAGAAGAACCCTGTGCTTCTAACTTCTTCCACACCACCCCACATCTTCTCCAAATGCTTTTAATTAATTTAGCAGGGCAAGATGGTTGTGTAGTTTCTTGTGCAAAATCTCCTTAATAAGTCTCCTTTTGCTTACTCCCCTTGACATTCCAAGAGAGAATCTTTATTAAGAAACTGAGCACCCCTATCTTCAATAGAAGCCTATCTTATGTCATAATTAATGGTAGATTCTGAATTAAGTTCCCTAAATCTTTTAACCTTACTATACTGCCGCCTAATTCCCACCCTCGCCTTTCTAGTCAAAGACTTAGACCTAGAATTTAGGGGAACAAGACGAAGACCAATCTTAGCCAAAAGCACTCAAATTTGACCATTCCTTACTAGACTGACCAGAATGATCTACCTCTCTTGACAGACCTGAGAAAAGTAAACTTCCCCTTGCCTCCAAACCAGGGAATGATATTCTCCTCTAACACCATGCACAAAGGCTCAGAATAATTAATATTATCTCTTCTTATTGCCTTAATAGGATAGAGGGAGCAAAATTGATAGCGGTTTAAATTTTGTTCCTTTCCATTTTTCTTGTGCTGCATGGTAGCATAAAATTCTATTAGAAGAGACTAGACCAATCCTTTTGCTGCATATTAATGTCAGTCCATGAATTGACGAAAGCAAATAATTACAGTTTCATGGATGATATTTCTTTTGAAAGCTTGATACTTCTTTCAACAGTTGGAGAGATTTCATTATTGAGAAACTTTATACTTTGACAGTGGCCGACATGGTTTTGTTGTGGCAATTACGGGAATTGAGAATATTGGGAAAGGATTAATTCGTGATGGCACAGGTTTTGTCACTTTTCCTGTTAAATACCAATGTGTTGTGTTTCGACCATTCAAGGGAGAAATCTTAGAAGCAGTTGTTACTATGGTAAACAAGGTAGGTATTTGCCACATTTATTAGCTATCAGCAAGTAATTAATGAAGCTCTTTTTAATAATAAATATGAATATGACCGCAACTGCAACTATTGTAGATGGGCTTTTTTGCTGAAGCTGGACCTGTTCAGATTTTTGTTTCAAACCATGTAAGTATATATTTACAAACTATTCCAGTTATCTTCTTTTTTCTCTGTGATGTTTTAAACCACCAAATATCTCCTTTTTTTTTTTTTTTTTTGTAATTTCATGACATCTTGAAATCGTTCCTTACCAAATATATGTGTGTGTGTGGTGGGACAGCATGGCAGTGATGCTCAGAGAGCTTTTTATATGCCAATTGAACTTTTTTCTTGTTCATAGAGTCGTACATGTATGAGAGGAGTAAACTGCACTATATGTAAAGTGGATTGTAAAAATTTCGAGAGAGAGATTCAGAATTCCTTACGGATGCTGGTACTCTAGTTCTAGTAGTGTTTGATGTAAAGTGAAGTAGGAAAGCAAGATAACACTTTTCTTATTAGTTTCTAGTTTGTACTTTTGATGCTTCTCAGTGTTACTGTGTCATGTTGAAATAAATAGAGTAAAGGGGGCGTTTTGTATGCAGGCTCAACTGTGAGATATGTTTGCACGATCTTATCCCTGTGCAATGCAAACACATGCATCTCAATCTCAACAATCCTATTACAAATTTCTCATTTCCAAGCTTTTCATTTAAACGCAAATCATACCCATTCCTGACTATAAATACAAACATCTTATTCCTAACAATCTTCTTACCAAGCTTTCCATTCTGGGACTTACGGTTTTTGGGCTTCATAATTCATGCCAACATACCAAACGCCTCCTGAGTTAACTTTTCTTAACTGTATTTGCAACACCAACATCTTGGTTTTTATTCCTATTATAAAAAATTTCCCCTTAATGTGCTTAATCTTCAATGAATTTATCCCACATCTTGTGTTTCAGTTGATACCAGATGATATGGAGTTCCAGTCTGGAGACGTGCCCAACTATACCACTTCGGATGGATCAGTATTTTTCTGTTTCACATGCCTTCTCTAACATCAACTGTTAATCTCTGTCGTTTTGTTAAATTTATTGAATACTTCTGTTCTTTTGTAGGTTAAGATTCAGAAGGACAGTGAAGTTCGATTAAAGATAATTGGGACTCGAGTAGATGCAACTGAAATT

mRNA sequence

ATGAGGCCTCTCAATAACCATTGTAGATCAAAATCTGGCATGTTTGATAATACCGGATATAAATTCAAAATATTTATTTTTTATATATCTAATGATATAAAACTACTAAAATTTCTCCAACTCCTACGTATTCCTTCCTCGGCACCCCACCCGCCCCCAAAAAAAAAAAAAAAATTTCTCCTACACGTTCCTCCTGCTCTCCCTCCCTCTTCGGCTTCTGCCGTCCCTCCCCGAAGCCCCTTCCATCGCGCTCAGTTCACCGTCTCCTACTTTCTTGCGTCGCCCCTACGATTTTTCTCAGAAGTTGCATTCATCAATGAGCTCAAATGGGTCTTTCCGCAGGTTGAGAGAGCCACCATGTTCTTCCACATAGTGTTGGAAAGGAATATGCAGCTGCATCCACGGCACTTTGGTCGTAACCTCCGTGAGAATTTGGTTACCAAACTCATGAAAGATGTCGAGGGCACTTGCAGTGGCCGACATGGTTTTGTTGTGGCAATTACGGGAATTGAGAATATTGGGAAAGGATTAATTCGTGATGGCACAGGTTTTGTCACTTTTCCTGTTAAATACCAATGTGTTGTGTTTCGACCATTCAAGGGAGAAATCTTAGAAGCAGTTGTTACTATGGTAAACAAGATGGGCTTTTTTGCTGAAGCTGGACCTGTTCAGATTTTTGTTTCAAACCATTTGATACCAGATGATATGGAGTTCCAGTCTGGAGACGTGCCCAACTATACCACTTCGGATGGATCAGTTAAGATTCAGAAGGACAGTGAAGTTCGATTAAAGATAATTGGGACTCGAGTAGATGCAACTGAAATT

Coding sequence (CDS)

ATGAGGCCTCTCAATAACCATTGTAGATCAAAATCTGGCATGTTTGATAATACCGGATATAAATTCAAAATATTTATTTTTTATATATCTAATGATATAAAACTACTAAAATTTCTCCAACTCCTACGTATTCCTTCCTCGGCACCCCACCCGCCCCCAAAAAAAAAAAAAAAATTTCTCCTACACGTTCCTCCTGCTCTCCCTCCCTCTTCGGCTTCTGCCGTCCCTCCCCGAAGCCCCTTCCATCGCGCTCAGTTCACCGTCTCCTACTTTCTTGCGTCGCCCCTACGATTTTTCTCAGAAGTTGCATTCATCAATGAGCTCAAATGGGTCTTTCCGCAGGTTGAGAGAGCCACCATGTTCTTCCACATAGTGTTGGAAAGGAATATGCAGCTGCATCCACGGCACTTTGGTCGTAACCTCCGTGAGAATTTGGTTACCAAACTCATGAAAGATGTCGAGGGCACTTGCAGTGGCCGACATGGTTTTGTTGTGGCAATTACGGGAATTGAGAATATTGGGAAAGGATTAATTCGTGATGGCACAGGTTTTGTCACTTTTCCTGTTAAATACCAATGTGTTGTGTTTCGACCATTCAAGGGAGAAATCTTAGAAGCAGTTGTTACTATGGTAAACAAGATGGGCTTTTTTGCTGAAGCTGGACCTGTTCAGATTTTTGTTTCAAACCATTTGATACCAGATGATATGGAGTTCCAGTCTGGAGACGTGCCCAACTATACCACTTCGGATGGATCAGTTAAGATTCAGAAGGACAGTGAAGTTCGATTAAAGATAATTGGGACTCGAGTAGATGCAACTGAAATT

Protein sequence

MRPLNNHCRSKSGMFDNTGYKFKIFIFYISNDIKLLKFLQLLRIPSSAPHPPPKKKKKFLLHVPPALPPSSASAVPPRSPFHRAQFTVSYFLASPLRFFSEVAFINELKWVFPQVERATMFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQSGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI
Homology
BLAST of Sgr016966 vs. NCBI nr
Match: KAG7032688.1 (hypothetical protein SDJN02_06738, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 322.8 bits (826), Expect = 2.9e-84
Identity = 159/161 (98.76%), Postives = 160/161 (99.38%), Query Frame = 0

Query: 115 VERATMFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIG 174
           V+ ATMFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIG
Sbjct: 1   VKGATMFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIG 60

Query: 175 KGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPD 234
           KGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPD
Sbjct: 61  KGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPD 120

Query: 235 DMEFQSGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 276
           DMEFQSGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI
Sbjct: 121 DMEFQSGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 161

BLAST of Sgr016966 vs. NCBI nr
Match: XP_022939214.1 (DNA-directed RNA polymerase II subunit RPB7 isoform X1 [Cucurbita moschata])

HSP 1 Score: 318.2 bits (814), Expect = 7.2e-83
Identity = 155/162 (95.68%), Postives = 160/162 (98.77%), Query Frame = 0

Query: 114 QVERATMFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENI 173
           +V+ ATMFFHIVLERNMQLHPRHFGRNLRENLV+KLMKDVEGTCSGRHGFVVAITGIENI
Sbjct: 67  KVQGATMFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENI 126

Query: 174 GKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIP 233
           GKGLI DGTGFVTFPVKYQCVVFRPFKGEILEA+VTMVNKMGFFAEAGPVQIFVSNHLIP
Sbjct: 127 GKGLISDGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNKMGFFAEAGPVQIFVSNHLIP 186

Query: 234 DDMEFQSGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 276
           DDMEFQSGD+PNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI
Sbjct: 187 DDMEFQSGDMPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 228

BLAST of Sgr016966 vs. NCBI nr
Match: XP_022923594.1 (DNA-directed RNA polymerase II subunit RPB7-like [Cucurbita moschata] >XP_022923602.1 DNA-directed RNA polymerase II subunit RPB7-like [Cucurbita moschata] >XP_022990163.1 DNA-directed RNA polymerase II subunit RPB7-like [Cucurbita maxima] >XP_022990164.1 DNA-directed RNA polymerase II subunit RPB7-like [Cucurbita maxima] >XP_023525602.1 DNA-directed RNA polymerase II subunit RPB7-like [Cucurbita pepo subsp. pepo] >KAG6601991.1 hypothetical protein SDJN03_07224, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 317.8 bits (813), Expect = 9.4e-83
Identity = 156/156 (100.00%), Postives = 156/156 (100.00%), Query Frame = 0

Query: 120 MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 179
           MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60

Query: 180 DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 239
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 240 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 276
           SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI
Sbjct: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 156

BLAST of Sgr016966 vs. NCBI nr
Match: KAG6579070.1 (hypothetical protein SDJN03_23518, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 317.8 bits (813), Expect = 9.4e-83
Identity = 155/161 (96.27%), Postives = 159/161 (98.76%), Query Frame = 0

Query: 115 VERATMFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIG 174
           V+ ATMFFHIVLERNMQLHPRHFGRNLRENLV+KLMKDVEGTCSGRHGFVVAITGIENIG
Sbjct: 80  VQGATMFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIG 139

Query: 175 KGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPD 234
           KGLI DGTGFVTFPVKYQCVVFRPFKGEILEA+VTMVNKMGFFAEAGPVQIFVSNHLIPD
Sbjct: 140 KGLISDGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNKMGFFAEAGPVQIFVSNHLIPD 199

Query: 235 DMEFQSGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 276
           DMEFQSGD+PNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI
Sbjct: 200 DMEFQSGDMPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 240

BLAST of Sgr016966 vs. NCBI nr
Match: XP_021894560.1 (DNA-directed RNA polymerase II subunit RPB7 [Carica papaya] >XP_021894561.1 DNA-directed RNA polymerase II subunit RPB7 [Carica papaya])

HSP 1 Score: 316.6 bits (810), Expect = 2.1e-82
Identity = 155/156 (99.36%), Postives = 156/156 (100.00%), Query Frame = 0

Query: 120 MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 179
           MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60

Query: 180 DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 239
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 240 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 276
           SGD+PNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI
Sbjct: 121 SGDMPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 156

BLAST of Sgr016966 vs. ExPASy Swiss-Prot
Match: P46279 (DNA-directed RNA polymerase II subunit RPB7 OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 1.1e-83
Identity = 151/156 (96.79%), Postives = 156/156 (100.00%), Query Frame = 0

Query: 120 MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 179
           MFFHIVLERNMQLHPR+FGRNLR+NLV+KLMKDVEGTCSGRHGFVVA+TGIENIGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRYFGRNLRDNLVSKLMKDVEGTCSGRHGFVVAVTGIENIGKGLIR 60

Query: 180 DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 239
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 240 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 276
           SGD+PNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI
Sbjct: 121 SGDMPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 156

BLAST of Sgr016966 vs. ExPASy Swiss-Prot
Match: P38421 (DNA-directed RNA polymerase II subunit 7 OS=Arabidopsis thaliana OX=3702 GN=NRPB7 PE=1 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 2.9e-79
Identity = 144/156 (92.31%), Postives = 151/156 (96.79%), Query Frame = 0

Query: 120 MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 179
           MFFHIVLERNMQLHPR FGRNL+ENLV+KLMKDVEGTCSGRHGFVVAITGI+ IGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRFFGRNLKENLVSKLMKDVEGTCSGRHGFVVAITGIDTIGKGLIR 60

Query: 180 DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 239
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVT+VNKMGFFAEAGPVQIFVS HLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTLVNKMGFFAEAGPVQIFVSKHLIPDDMEFQ 120

Query: 240 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 276
           +GD+PNYTTSDGSVKIQK+ EVRLKIIGTRVDAT I
Sbjct: 121 AGDMPNYTTSDGSVKIQKECEVRLKIIGTRVDATAI 156

BLAST of Sgr016966 vs. ExPASy Swiss-Prot
Match: Q5E9B8 (DNA-directed RNA polymerase II subunit RPB7 OS=Bos taurus OX=9913 GN=POLR2G PE=1 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 5.0e-47
Identity = 87/157 (55.41%), Postives = 117/157 (74.52%), Query Frame = 0

Query: 120 MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 179
           MF+HI LE  + LHPR+FG NL   +  KL  +VEGTC+G++GFV+A+T I+NIG G+I+
Sbjct: 1   MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQ 60

Query: 180 DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 239
            G GFV +PVKY+ +VFRPFKGE+++AVVT VNK+G F E GP+  F+S H IP +MEF 
Sbjct: 61  PGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFD 120

Query: 240 -SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 276
            + + P Y T D  + IQ+D E+RLKI+GTRVD  +I
Sbjct: 121 PNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDI 157

BLAST of Sgr016966 vs. ExPASy Swiss-Prot
Match: Q7ZW41 (DNA-directed RNA polymerase II subunit RPB7 OS=Danio rerio OX=7955 GN=polr2g PE=2 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 5.0e-47
Identity = 88/157 (56.05%), Postives = 117/157 (74.52%), Query Frame = 0

Query: 120 MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 179
           MF+HI LE  + LHPR+FG NL   +  KL  +VEGTC+G++GFV+A+T I+NIG G+I+
Sbjct: 1   MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQ 60

Query: 180 DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 239
            G GFV +PVKY+ +VFRPFKGE+++AVVT VNK+G F E GP+  F+S H IP +MEF 
Sbjct: 61  PGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFD 120

Query: 240 -SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 276
            + + P Y T D  V IQ+D E+RLKI+GTRVD  +I
Sbjct: 121 PNSNPPCYKTVDEDVVIQQDDEIRLKIVGTRVDKNDI 157

BLAST of Sgr016966 vs. ExPASy Swiss-Prot
Match: P62487 (DNA-directed RNA polymerase II subunit RPB7 OS=Homo sapiens OX=9606 GN=POLR2G PE=1 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 5.0e-47
Identity = 87/157 (55.41%), Postives = 117/157 (74.52%), Query Frame = 0

Query: 120 MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 179
           MF+HI LE  + LHPR+FG NL   +  KL  +VEGTC+G++GFV+A+T I+NIG G+I+
Sbjct: 1   MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQ 60

Query: 180 DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 239
            G GFV +PVKY+ +VFRPFKGE+++AVVT VNK+G F E GP+  F+S H IP +MEF 
Sbjct: 61  PGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFD 120

Query: 240 -SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 276
            + + P Y T D  + IQ+D E+RLKI+GTRVD  +I
Sbjct: 121 PNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDI 157

BLAST of Sgr016966 vs. ExPASy TrEMBL
Match: A0A6J1FGH6 (DNA-directed RNA polymerase II subunit RPB7 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445199 PE=3 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 3.5e-83
Identity = 155/162 (95.68%), Postives = 160/162 (98.77%), Query Frame = 0

Query: 114 QVERATMFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENI 173
           +V+ ATMFFHIVLERNMQLHPRHFGRNLRENLV+KLMKDVEGTCSGRHGFVVAITGIENI
Sbjct: 67  KVQGATMFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENI 126

Query: 174 GKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIP 233
           GKGLI DGTGFVTFPVKYQCVVFRPFKGEILEA+VTMVNKMGFFAEAGPVQIFVSNHLIP
Sbjct: 127 GKGLISDGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNKMGFFAEAGPVQIFVSNHLIP 186

Query: 234 DDMEFQSGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 276
           DDMEFQSGD+PNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI
Sbjct: 187 DDMEFQSGDMPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 228

BLAST of Sgr016966 vs. ExPASy TrEMBL
Match: A0A6J1JSG6 (DNA-directed RNA polymerase II subunit RPB7-like OS=Cucurbita maxima OX=3661 GN=LOC111487136 PE=3 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 4.5e-83
Identity = 156/156 (100.00%), Postives = 156/156 (100.00%), Query Frame = 0

Query: 120 MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 179
           MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60

Query: 180 DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 239
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 240 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 276
           SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI
Sbjct: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 156

BLAST of Sgr016966 vs. ExPASy TrEMBL
Match: A0A6J1E6V3 (DNA-directed RNA polymerase II subunit RPB7-like OS=Cucurbita moschata OX=3662 GN=LOC111431229 PE=3 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 4.5e-83
Identity = 156/156 (100.00%), Postives = 156/156 (100.00%), Query Frame = 0

Query: 120 MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 179
           MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60

Query: 180 DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 239
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 240 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 276
           SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI
Sbjct: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 156

BLAST of Sgr016966 vs. ExPASy TrEMBL
Match: A0A0A0KPS4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G312340 PE=3 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 1.3e-82
Identity = 155/156 (99.36%), Postives = 156/156 (100.00%), Query Frame = 0

Query: 120 MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 179
           MFFHIVLERNMQLHPRHFGRNLRENLV+KLMKDVEGTCSGRHGFVVAITGIENIGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60

Query: 180 DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 239
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 240 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 276
           SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI
Sbjct: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 156

BLAST of Sgr016966 vs. ExPASy TrEMBL
Match: A0A6J0ZV58 (DNA-directed RNA polymerase II subunit RPB7 OS=Herrania umbratica OX=108875 GN=LOC110412415 PE=3 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 3.8e-82
Identity = 153/156 (98.08%), Postives = 156/156 (100.00%), Query Frame = 0

Query: 120 MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 179
           MFFHIVLERNMQLHPRHFGRNLRENLV+KLMKDVEGTCSGRHGFVVAITGIEN+GKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENVGKGLIR 60

Query: 180 DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 239
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 240 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 276
           SGD+PNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI
Sbjct: 121 SGDMPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 156

BLAST of Sgr016966 vs. TAIR 10
Match: AT5G59180.1 (DNA-directed RNA polymerase II )

HSP 1 Score: 296.6 bits (758), Expect = 2.1e-80
Identity = 144/156 (92.31%), Postives = 151/156 (96.79%), Query Frame = 0

Query: 120 MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 179
           MFFHIVLERNMQLHPR FGRNL+ENLV+KLMKDVEGTCSGRHGFVVAITGI+ IGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRFFGRNLKENLVSKLMKDVEGTCSGRHGFVVAITGIDTIGKGLIR 60

Query: 180 DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 239
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVT+VNKMGFFAEAGPVQIFVS HLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTLVNKMGFFAEAGPVQIFVSKHLIPDDMEFQ 120

Query: 240 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI 276
           +GD+PNYTTSDGSVKIQK+ EVRLKIIGTRVDAT I
Sbjct: 121 AGDMPNYTTSDGSVKIQKECEVRLKIIGTRVDATAI 156

BLAST of Sgr016966 vs. TAIR 10
Match: AT3G22900.1 (RNA polymerase Rpb7-like, N-terminal domain )

HSP 1 Score: 73.6 bits (179), Expect = 2.9e-13
Identity = 39/107 (36.45%), Postives = 63/107 (58.88%), Query Frame = 0

Query: 162 GFVVAITGIENIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAG 221
           G+++  T +ENIG+G I++ TG + FPV +  + F+ FKGEI+  VV  V+K G F ++G
Sbjct: 46  GYLITPTILENIGEGKIKEQTGEIQFPVVFNGICFKMFKGEIVHGVVHKVHKTGVFLKSG 105

Query: 222 PVQIFVSNHLIPDDMEFQSGDVPNYTTSDGSVKIQKDSEVRLKIIGT 269
           P +I   +H+     EF  G+ P +     S +IQ  + VR  ++ T
Sbjct: 106 PYEIIYLSHMKMPGYEFIPGENPFFMNQYMS-RIQIGARVRFVVLDT 151

BLAST of Sgr016966 vs. TAIR 10
Match: AT4G14660.1 (RNA polymerase Rpb7-like, N-terminal domain )

HSP 1 Score: 73.6 bits (179), Expect = 2.9e-13
Identity = 46/154 (29.87%), Postives = 82/154 (53.25%), Query Frame = 0

Query: 120 MFFHIVLERNMQLHPRHF---GRNLRENLVTKLMKDVEGTCSGRH-GFVVAITGIENIGK 179
           MF  + L  N+ +   +    G  L+  ++ +L++      + +  G+ VA+T ++ IG+
Sbjct: 1   MFLKVQLPWNVMIPAENMDAKGLMLKRAILVELLEAFASKKATKELGYYVAVTTLDKIGE 60

Query: 180 GLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDD 239
           G IR+ TG V FPV +  + F+ FKGEI+  VV  V K G F   GP++    ++    D
Sbjct: 61  GKIREHTGEVLFPVMFSGMTFKIFKGEIIHGVVHKVLKHGVFMRCGPIENVYLSYTKMPD 120

Query: 240 MEFQSGDVPNYTTSDGSVKIQKDSEVRLKIIGTR 270
            ++  G+ P +     S +IQ ++ VR+ +IG +
Sbjct: 121 YKYIPGENPIFMNEKTS-RIQVETTVRVVVIGIK 153

BLAST of Sgr016966 vs. TAIR 10
Match: AT1G06790.2 (RNA polymerase Rpb7 N-terminal domain-containing protein )

HSP 1 Score: 43.1 bits (100), Expect = 4.1e-04
Identity = 25/87 (28.74%), Postives = 42/87 (48.28%), Query Frame = 0

Query: 120 MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 179
           MF+   LE ++++ P      L + + + L            G  V+I  I+++  G + 
Sbjct: 1   MFYLSELEHSLRVPPHLLNLPLEDAIKSVLQNVFLDKVLADLGLCVSIYDIKSVEGGFVL 60

Query: 180 DGTGFVTFPVKYQCVVFRPFKGEILEA 207
            G G  T+ V  + VVFRPF GE++ A
Sbjct: 61  PGDGAATYKVGLRIVVFRPFVGEVIAA 87

BLAST of Sgr016966 vs. TAIR 10
Match: AT1G06790.1 (RNA polymerase Rpb7 N-terminal domain-containing protein )

HSP 1 Score: 43.1 bits (100), Expect = 4.1e-04
Identity = 25/87 (28.74%), Postives = 42/87 (48.28%), Query Frame = 0

Query: 120 MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 179
           MF+   LE ++++ P      L + + + L            G  V+I  I+++  G + 
Sbjct: 1   MFYLSELEHSLRVPPHLLNLPLEDAIKSVLQNVFLDKVLADLGLCVSIYDIKSVEGGFVL 60

Query: 180 DGTGFVTFPVKYQCVVFRPFKGEILEA 207
            G G  T+ V  + VVFRPF GE++ A
Sbjct: 61  PGDGAATYKVGLRIVVFRPFVGEVIAA 87

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7032688.12.9e-8498.76hypothetical protein SDJN02_06738, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022939214.17.2e-8395.68DNA-directed RNA polymerase II subunit RPB7 isoform X1 [Cucurbita moschata][more]
XP_022923594.19.4e-83100.00DNA-directed RNA polymerase II subunit RPB7-like [Cucurbita moschata] >XP_022923... [more]
KAG6579070.19.4e-8396.27hypothetical protein SDJN03_23518, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_021894560.12.1e-8299.36DNA-directed RNA polymerase II subunit RPB7 [Carica papaya] >XP_021894561.1 DNA-... [more]
Match NameE-valueIdentityDescription
P462791.1e-8396.79DNA-directed RNA polymerase II subunit RPB7 OS=Glycine max OX=3847 PE=2 SV=1[more]
P384212.9e-7992.31DNA-directed RNA polymerase II subunit 7 OS=Arabidopsis thaliana OX=3702 GN=NRPB... [more]
Q5E9B85.0e-4755.41DNA-directed RNA polymerase II subunit RPB7 OS=Bos taurus OX=9913 GN=POLR2G PE=1... [more]
Q7ZW415.0e-4756.05DNA-directed RNA polymerase II subunit RPB7 OS=Danio rerio OX=7955 GN=polr2g PE=... [more]
P624875.0e-4755.41DNA-directed RNA polymerase II subunit RPB7 OS=Homo sapiens OX=9606 GN=POLR2G PE... [more]
Match NameE-valueIdentityDescription
A0A6J1FGH63.5e-8395.68DNA-directed RNA polymerase II subunit RPB7 isoform X1 OS=Cucurbita moschata OX=... [more]
A0A6J1JSG64.5e-83100.00DNA-directed RNA polymerase II subunit RPB7-like OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1E6V34.5e-83100.00DNA-directed RNA polymerase II subunit RPB7-like OS=Cucurbita moschata OX=3662 G... [more]
A0A0A0KPS41.3e-8299.36Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G312340 PE=3 SV=1[more]
A0A6J0ZV583.8e-8298.08DNA-directed RNA polymerase II subunit RPB7 OS=Herrania umbratica OX=108875 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G59180.12.1e-8092.31DNA-directed RNA polymerase II [more]
AT3G22900.12.9e-1336.45RNA polymerase Rpb7-like, N-terminal domain [more]
AT4G14660.12.9e-1329.87RNA polymerase Rpb7-like, N-terminal domain [more]
AT1G06790.24.1e-0428.74RNA polymerase Rpb7 N-terminal domain-containing protein [more]
AT1G06790.14.1e-0428.74RNA polymerase Rpb7 N-terminal domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003029S1 domainPFAMPF00575S1coord: 197..275
e-value: 8.7E-16
score: 58.0
IPR036898RNA polymerase Rpb7-like, N-terminal domain superfamilyGENE3D3.30.1490.120coord: 119..199
e-value: 6.8E-28
score: 98.8
IPR036898RNA polymerase Rpb7-like, N-terminal domain superfamilySUPERFAMILY88798N-terminal, heterodimerisation domain of RBP7 (RpoE)coord: 120..197
NoneNo IPR availableGENE3D2.40.50.140coord: 200..275
e-value: 1.4E-27
score: 97.5
NoneNo IPR availablePANTHERPTHR12709:SF8BNAA10G12180D PROTEINcoord: 108..275
NoneNo IPR availableCDDcd04329RNAP_II_Rpb7_Ncoord: 121..200
e-value: 1.28659E-39
score: 131.15
NoneNo IPR availableCDDcd04462S1_RNAPII_Rpb7coord: 200..275
e-value: 2.21376E-36
score: 122.755
IPR005576RNA polymerase Rpb7-like , N-terminalPFAMPF03876SHS2_Rpb7-Ncoord: 127..183
e-value: 1.4E-12
score: 47.7
IPR045113RNA polymerase Rpb7-likePANTHERPTHR12709DNA-DIRECTED RNA POLYMERASE II, IIIcoord: 108..275
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 197..274

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr016966.1Sgr016966.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000291 nuclear-transcribed mRNA catabolic process, exonucleolytic
biological_process GO:0060213 positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
biological_process GO:0045948 positive regulation of translational initiation
biological_process GO:0006367 transcription initiation from RNA polymerase II promoter
biological_process GO:0006352 DNA-templated transcription, initiation
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0000932 P-body
cellular_component GO:0005665 RNA polymerase II, core complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0003697 single-stranded DNA binding
molecular_function GO:0003727 single-stranded RNA binding
molecular_function GO:0031369 translation initiation factor binding
molecular_function GO:0003676 nucleic acid binding