Sgr014951 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr014951
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationtig00002486: 434835 .. 435938 (+)
RNA-Seq ExpressionSgr014951
SyntenySgr014951
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCTGTGGGAAACTCAAATCAATGGCAGCCATATGCTTACAAGGACTGTTCTTTGGAGATTTGCAGCATATATTGCCCTCAGTGGTGCTACATCATCTTCCCACCACCTCCACCTTTTGGCTATGGCAACAATGGCGATTCTGGCACTGATTTCTCCCCTCTCATCATCGCCATCATTGGAATCTTGGCCAGCGCCTTCATCTTGGTAAGCTACTACACCATCATCTCCAAGTACTGCAGGAACAGAGCTGTCACCACCGCCGCCATGGAAATGGATGATGAAGAAAATCTTGCCCACATCCGCCATGAAAACCAGCACCAAGCTCCTCCTGCAGGCTTGGACGAGGCTCTCATCAAGTCCATCACAGTCTGCAAGTACAAAAGAGGCGACGGGTTAGTTGAAGGCACCGACTGCTCCGTTTGCCTGAGTGATTTTCAAGAAAATGAGAGCCTGAGGCTGCTACCAAAGTGCAGCCATGCCTTTCATCTTCCCTGCATCGACACATGGCTCAAATCTCACTCAACTTGCCCTCTCTGCCGCTCCATCATTGCACCCACCAATCTTTTCCCACCCCCAACTCAAGAAATCCAAAGAACAGAACACTTAAATGCATCTGCTCTTCAATATCAGCACAGAAGCAATGACACAGTTGTTGTAGTGGTTCAAGATTTGCAGGGTTTAACAATGAGACAAGAAACTGCTGTTTTGGGGCTTGAAAATGATGATGCTTCTTTCAAGAATCAAAGGCAAAGTTTTGCATCAGATAGTCAAGATTCAGAGCCAAGAGACAGAATGATTCAAGTTACACAAGATGATGTTGTTCAGCCATTTAGAAGGTCAGTTTCCTTGGATTCTTTTTCATGGCAAGGGCAGGTTTCTGTTGCTGACATATTGAGAGCCAGTCAAGATAGTGAGGAAGAGGTAGAGGAAGATGAATTGCAAATGGGAATTGGATCTTCAAAAGTATTTGTTCAAGAGCATTGCCAATCCAATTACAAAACTGGGGTTTCCAACTTGGGAATGAACAGATCTATCTCAACCGGGAGACTGGGCTTTACAAACTATGGAAAAGGAAAGAGTTGCATCAATCCCAGTTGA

mRNA sequence

ATGGGTTCTGTGGGAAACTCAAATCAATGGCAGCCATATGCTTACAAGGACTGTTCTTTGGAGATTTGCAGCATATATTGCCCTCAGTGGTGCTACATCATCTTCCCACCACCTCCACCTTTTGGCTATGGCAACAATGGCGATTCTGGCACTGATTTCTCCCCTCTCATCATCGCCATCATTGGAATCTTGGCCAGCGCCTTCATCTTGGTAAGCTACTACACCATCATCTCCAAGTACTGCAGGAACAGAGCTGTCACCACCGCCGCCATGGAAATGGATGATGAAGAAAATCTTGCCCACATCCGCCATGAAAACCAGCACCAAGCTCCTCCTGCAGGCTTGGACGAGGCTCTCATCAAGTCCATCACAGTCTGCAAGTACAAAAGAGGCGACGGGTTAGTTGAAGGCACCGACTGCTCCGTTTGCCTGAGTGATTTTCAAGAAAATGAGAGCCTGAGGCTGCTACCAAAGTGCAGCCATGCCTTTCATCTTCCCTGCATCGACACATGGCTCAAATCTCACTCAACTTGCCCTCTCTGCCGCTCCATCATTGCACCCACCAATCTTTTCCCACCCCCAACTCAAGAAATCCAAAGAACAGAACACTTAAATGCATCTGCTCTTCAATATCAGCACAGAAGCAATGACACAGTTGTTGTAGTGGTTCAAGATTTGCAGGGTTTAACAATGAGACAAGAAACTGCTGTTTTGGGGCTTGAAAATGATGATGCTTCTTTCAAGAATCAAAGGCAAAGTTTTGCATCAGATAGTCAAGATTCAGAGCCAAGAGACAGAATGATTCAAGTTACACAAGATGATGTTGTTCAGCCATTTAGAAGGTCAGTTTCCTTGGATTCTTTTTCATGGCAAGGGCAGGTTTCTGTTGCTGACATATTGAGAGCCAGTCAAGATAGTGAGGAAGAGGTAGAGGAAGATGAATTGCAAATGGGAATTGGATCTTCAAAAGTATTTGTTCAAGAGCATTGCCAATCCAATTACAAAACTGGGGTTTCCAACTTGGGAATGAACAGATCTATCTCAACCGGGAGACTGGGCTTTACAAACTATGGAAAAGGAAAGAGTTGCATCAATCCCAGTTGA

Coding sequence (CDS)

ATGGGTTCTGTGGGAAACTCAAATCAATGGCAGCCATATGCTTACAAGGACTGTTCTTTGGAGATTTGCAGCATATATTGCCCTCAGTGGTGCTACATCATCTTCCCACCACCTCCACCTTTTGGCTATGGCAACAATGGCGATTCTGGCACTGATTTCTCCCCTCTCATCATCGCCATCATTGGAATCTTGGCCAGCGCCTTCATCTTGGTAAGCTACTACACCATCATCTCCAAGTACTGCAGGAACAGAGCTGTCACCACCGCCGCCATGGAAATGGATGATGAAGAAAATCTTGCCCACATCCGCCATGAAAACCAGCACCAAGCTCCTCCTGCAGGCTTGGACGAGGCTCTCATCAAGTCCATCACAGTCTGCAAGTACAAAAGAGGCGACGGGTTAGTTGAAGGCACCGACTGCTCCGTTTGCCTGAGTGATTTTCAAGAAAATGAGAGCCTGAGGCTGCTACCAAAGTGCAGCCATGCCTTTCATCTTCCCTGCATCGACACATGGCTCAAATCTCACTCAACTTGCCCTCTCTGCCGCTCCATCATTGCACCCACCAATCTTTTCCCACCCCCAACTCAAGAAATCCAAAGAACAGAACACTTAAATGCATCTGCTCTTCAATATCAGCACAGAAGCAATGACACAGTTGTTGTAGTGGTTCAAGATTTGCAGGGTTTAACAATGAGACAAGAAACTGCTGTTTTGGGGCTTGAAAATGATGATGCTTCTTTCAAGAATCAAAGGCAAAGTTTTGCATCAGATAGTCAAGATTCAGAGCCAAGAGACAGAATGATTCAAGTTACACAAGATGATGTTGTTCAGCCATTTAGAAGGTCAGTTTCCTTGGATTCTTTTTCATGGCAAGGGCAGGTTTCTGTTGCTGACATATTGAGAGCCAGTCAAGATAGTGAGGAAGAGGTAGAGGAAGATGAATTGCAAATGGGAATTGGATCTTCAAAAGTATTTGTTCAAGAGCATTGCCAATCCAATTACAAAACTGGGGTTTCCAACTTGGGAATGAACAGATCTATCTCAACCGGGAGACTGGGCTTTACAAACTATGGAAAAGGAAAGAGTTGCATCAATCCCAGTTGA

Protein sequence

MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAIIGILASAFILVSYYTIISKYCRNRAVTTAAMEMDDEENLAHIRHENQHQAPPAGLDEALIKSITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCSHAFHLPCIDTWLKSHSTCPLCRSIIAPTNLFPPPTQEIQRTEHLNASALQYQHRSNDTVVVVVQDLQGLTMRQETAVLGLENDDASFKNQRQSFASDSQDSEPRDRMIQVTQDDVVQPFRRSVSLDSFSWQGQVSVADILRASQDSEEEVEEDELQMGIGSSKVFVQEHCQSNYKTGVSNLGMNRSISTGRLGFTNYGKGKSCINPS
Homology
BLAST of Sgr014951 vs. NCBI nr
Match: XP_022147036.1 (RING-H2 finger protein ATL52-like [Momordica charantia])

HSP 1 Score: 673.7 bits (1737), Expect = 9.0e-190
Identity = 340/373 (91.15%), Postives = 351/373 (94.10%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRAVTT-AAMEMDDEENLAHIRHENQHQAPPAGLDEAL 120
           IGILASAFILVSYYTIISKYCRNRA TT AAMEMDDEENLA IRHENQ QAPPAGLDEAL
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRAATTSAAMEMDDEENLAQIRHENQLQAPPAGLDEAL 120

Query: 121 IKSITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCSHAFHLPCIDTWLKSHSTCP 180
           IKSITVCKY RGDGLVEGTDCSVCLS+FQENESLRLLPKCSHAFHLPCIDTWLKSHSTCP
Sbjct: 121 IKSITVCKYIRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHSTCP 180

Query: 181 LCRSIIAPTNLFPPPTQEIQRTEHLNASALQYQHRSNDT-VVVVVQDLQGLTMRQETAVL 240
           LCRS I+PTNLFPPPTQEIQRTEH+NA+ALQYQHRSNDT VVVVVQDLQ LT+ Q+TAVL
Sbjct: 181 LCRSNISPTNLFPPPTQEIQRTEHINAAALQYQHRSNDTVVVVVVQDLQDLTVSQQTAVL 240

Query: 241 GLENDDASFKNQRQSFASDSQDSEPRDRMIQVTQD----DVVQPFRRSVSLDSFSWQGQV 300
           G+END AS KNQRQSFAS SQDSEPRDRMIQV +D    DVVQPFRRSVSLDSFSWQGQV
Sbjct: 241 GIENDGASSKNQRQSFASVSQDSEPRDRMIQVREDDVVGDVVQPFRRSVSLDSFSWQGQV 300

Query: 301 SVADILRASQDSEEEVEEDELQMGIGSSKVFVQEHCQSNYKTGVSNLGMNRSISTGRLGF 360
           SVADILR SQDSEEEVEEDE+QMGIGSSKVFVQEH QSN+K GVSNLGMNRS+STGRLGF
Sbjct: 301 SVADILRESQDSEEEVEEDEMQMGIGSSKVFVQEHFQSNHKNGVSNLGMNRSVSTGRLGF 360

Query: 361 TNYGKGKSCINPS 368
           TNY KGKSCI PS
Sbjct: 361 TNYEKGKSCIIPS 373

BLAST of Sgr014951 vs. NCBI nr
Match: XP_022980342.1 (RING-H2 finger protein ATL52-like [Cucurbita maxima])

HSP 1 Score: 624.0 bits (1608), Expect = 8.1e-175
Identity = 319/370 (86.22%), Postives = 333/370 (90.00%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAY DCSLEICSIYCPQWCYIIFPPPPPFGYG+NGDS TDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYNDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRAVTT-AAMEMDDEENLAHIRHENQHQA-PPAGLDEA 120
           IGILASAFI+VSYYTIISKYCRNRA TT AAMEMDDEENL HIRHENQ QA PPAGLD A
Sbjct: 61  IGILASAFIMVSYYTIISKYCRNRAATTNAAMEMDDEENLTHIRHENQLQAPPPAGLDHA 120

Query: 121 LIKSITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCSHAFHLPCIDTWLKSHSTC 180
           LIKSITVCKYKRGD LVEGTDCSVCLS+FQENESLRLLPKCSHAFHLPCIDTWLKSHSTC
Sbjct: 121 LIKSITVCKYKRGDDLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHSTC 180

Query: 181 PLCRSIIAPTNLFPPPTQEIQRTEHLNASALQYQHRSNDT-VVVVVQDLQGLTMRQETAV 240
           PLCRS I+PTN+ PPPTQEI RTEHLNASALQYQHR+NDT VVVVVQDLQ LT+RQETAV
Sbjct: 181 PLCRSNISPTNVSPPPTQEILRTEHLNASALQYQHRTNDTVVVVVVQDLQDLTVRQETAV 240

Query: 241 LGLENDDASFKNQRQSFASDSQDSEPRDRMIQVTQDDVVQPFRRSVSLDSFSWQGQVSVA 300
            GLENDDAS KN+RQSFASD QDSEPRDRM Q      VQP+RRSVSL+S SWQGQVSVA
Sbjct: 241 SGLENDDASSKNRRQSFASDCQDSEPRDRMNQ------VQPYRRSVSLNSLSWQGQVSVA 300

Query: 301 DILRASQDSEEEVEEDELQMGIGSSKVFVQEHCQSNYKTGVSNLGMNRSISTGRLGFTNY 360
           DILRASQDSE+EVEEDELQMGIGSSK+F QEHC         +LGMNRSISTG+ GFTNY
Sbjct: 301 DILRASQDSEDEVEEDELQMGIGSSKLFTQEHCH--------DLGMNRSISTGKQGFTNY 356

Query: 361 GKGKSCINPS 368
           GKGKSCI PS
Sbjct: 361 GKGKSCIIPS 356

BLAST of Sgr014951 vs. NCBI nr
Match: XP_023540121.1 (RING-H2 finger protein ATL51-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 617.5 bits (1591), Expect = 7.6e-173
Identity = 321/375 (85.60%), Postives = 335/375 (89.33%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAY+DCSLEICSIYCPQWCYIIFPPPPPFGYG N DSGTDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYRDCSLEICSIYCPQWCYIIFPPPPPFGYGTNSDSGTDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRAVTT-AAMEMDDEENLAHIRHENQHQA--PPAGLDE 120
           IGILASAFILVSYYTIISKYCRNRA T+ + MEMDDEENL+ IRHENQ QA  PPAGLDE
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRATTSNSNMEMDDEENLSQIRHENQLQAPPPPAGLDE 120

Query: 121 ALIKSITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCSHAFHLPCIDTWLKSHST 180
           ALIKSITVCKYKRGDGLVEGTDCSVCLS+F+ENESLRLLPKCSHAFHLPCIDTWLKSHST
Sbjct: 121 ALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHST 180

Query: 181 CPLCRSIIAPTNLFPPPTQEIQRTEHLNASALQYQHRSNDT-VVVVVQDLQGLTM-RQET 240
           CPLCRS I+P NLFPPPTQEIQRTEHLN+   QYQHRSNDT VVVVVQDL  LT+  QET
Sbjct: 181 CPLCRSNISPINLFPPPTQEIQRTEHLNSYNFQYQHRSNDTVVVVVVQDLPDLTVSSQET 240

Query: 241 AVLGLENDDASFKNQRQSFASDSQDSEPRDRMI-QVTQDDVVQPFRRSVSLDSFSWQGQV 300
           AVLGLEND  S KNQRQSF SD+QDSEPRD MI Q+ QDD VQPFRRSVSL+S SWQGQV
Sbjct: 241 AVLGLENDGVSSKNQRQSFVSDNQDSEPRDIMIHQIRQDDAVQPFRRSVSLNSLSWQGQV 300

Query: 301 SVADILRASQDSEEEVEEDELQMGIGSSKVFVQEHCQSNYKTGVSNLGMNRSISTGRLGF 360
           SVADILRASQDSEEEVEEDELQMGIGSSK FVQE C S     VSNL M+RSISTG+LGF
Sbjct: 301 SVADILRASQDSEEEVEEDELQMGIGSSKAFVQEDCHSR----VSNLEMHRSISTGKLGF 360

Query: 361 TNYG--KGKSCINPS 368
           TNYG  KGKSCI PS
Sbjct: 361 TNYGNWKGKSCIIPS 371

BLAST of Sgr014951 vs. NCBI nr
Match: KAG7028733.1 (RING-H2 finger protein ATL51, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 615.1 bits (1585), Expect = 3.8e-172
Identity = 320/374 (85.56%), Postives = 334/374 (89.30%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAY+DCSLEICSIYCPQWCYIIFPPPPPFGYG N DSGTDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYRDCSLEICSIYCPQWCYIIFPPPPPFGYGANSDSGTDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRAVTT-AAMEMDDEENLAHIRHENQHQA--PPAGLDE 120
           IGILASAFILVSYYTIISKYCRNRA T+ + MEMDDEENL+ IRHENQ QA  PPAGLDE
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRATTSNSNMEMDDEENLSQIRHENQLQAPPPPAGLDE 120

Query: 121 ALIKSITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCSHAFHLPCIDTWLKSHST 180
           ALIKSITVCKYKRGDGLVEGTDCSVCLS+F+ENESLRLLPKCSHAFHLPCIDTWLKSHST
Sbjct: 121 ALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHST 180

Query: 181 CPLCRSIIAPTNLFPPPTQEIQRTEHLNASALQYQHRSNDT-VVVVVQDLQGLTM-RQET 240
           CPLCRS I+P NLFPPPTQEIQRTEHLN+   QYQHRSNDT VVVVVQDL  LT+  QET
Sbjct: 181 CPLCRSNISPINLFPPPTQEIQRTEHLNSYNFQYQHRSNDTVVVVVVQDLPDLTVSSQET 240

Query: 241 AVLGLENDDASFKNQRQSFASDSQDSEPRDRMI-QVTQDDVVQPFRRSVSLDSFSWQGQV 300
           AVLGLEND  S KNQRQSF SD+QDSEPRD MI Q+ QDD VQPFRRSVSL+S SWQGQV
Sbjct: 241 AVLGLENDGVSSKNQRQSFVSDNQDSEPRDIMIHQIRQDDAVQPFRRSVSLNSLSWQGQV 300

Query: 301 SVADILRASQDSEEEVEEDELQMGIGSSKVFVQEHCQSNYKTGVSNLGMNRSISTGRLGF 360
           SVADILRASQDSEEEVEEDELQMGIGSSK FVQE C S     VSNL M+RSISTG+LGF
Sbjct: 301 SVADILRASQDSEEEVEEDELQMGIGSSKAFVQEDCHSR----VSNLEMHRSISTGKLGF 360

Query: 361 TNYG--KGKSCINP 367
           TNYG  KGKSCI P
Sbjct: 361 TNYGNWKGKSCIIP 370

BLAST of Sgr014951 vs. NCBI nr
Match: KAG6597262.1 (RING-H2 finger protein ATL51, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 614.8 bits (1584), Expect = 4.9e-172
Identity = 320/374 (85.56%), Postives = 334/374 (89.30%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAY+DCSLEICSIYCPQWCYIIFPPPPPFGYG N DSGTDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYRDCSLEICSIYCPQWCYIIFPPPPPFGYGANNDSGTDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRAVTT-AAMEMDDEENLAHIRHENQHQA--PPAGLDE 120
           IGILASAFILVSYYTIISKYCRNRA T+ + MEMDDEENL+ IRHENQ QA  PPAGLDE
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRATTSNSNMEMDDEENLSQIRHENQLQAPPPPAGLDE 120

Query: 121 ALIKSITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCSHAFHLPCIDTWLKSHST 180
           ALIKSITVCKYKRGDGLVEGTDCSVCLS+F+ENESLRLLPKCSHAFHLPCIDTWLKSHST
Sbjct: 121 ALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHST 180

Query: 181 CPLCRSIIAPTNLFPPPTQEIQRTEHLNASALQYQHRSNDT-VVVVVQDLQGLTM-RQET 240
           CPLCRS I+P NLFPPPTQEIQRTEHLN+   QYQHRSNDT VVVVVQDL  LT+  QET
Sbjct: 181 CPLCRSNISPINLFPPPTQEIQRTEHLNSYNFQYQHRSNDTVVVVVVQDLPDLTVSSQET 240

Query: 241 AVLGLENDDASFKNQRQSFASDSQDSEPRDRMI-QVTQDDVVQPFRRSVSLDSFSWQGQV 300
           AVLGLEND  S KNQRQSF SD+QDSEPRD MI Q+ QDD VQPFRRSVSL+S SWQGQV
Sbjct: 241 AVLGLENDGVSSKNQRQSFVSDNQDSEPRDIMIHQIRQDDAVQPFRRSVSLNSLSWQGQV 300

Query: 301 SVADILRASQDSEEEVEEDELQMGIGSSKVFVQEHCQSNYKTGVSNLGMNRSISTGRLGF 360
           SVADILRASQDSEEEVEEDELQMGIGSSK FVQE C S     VSNL M+RSISTG+LGF
Sbjct: 301 SVADILRASQDSEEEVEEDELQMGIGSSKAFVQEDCYSR----VSNLEMHRSISTGKLGF 360

Query: 361 TNYG--KGKSCINP 367
           TNYG  KGKSCI P
Sbjct: 361 TNYGNWKGKSCIIP 370

BLAST of Sgr014951 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.2e-67
Identity = 163/389 (41.90%), Postives = 222/389 (57.07%), Query Frame = 0

Query: 6   NSNQWQPY-AYKDCSLEICSIYCPQWCYIIFPPPPP--FGYGNNGDSGTDFSPLIIAIIG 65
           N N W PY +Y DCS  IC+IYCPQWCY+IFPPPPP  F   ++  S + FSPL+IA+IG
Sbjct: 4   NPNPWSPYDSYNDCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALIG 63

Query: 66  ILASAFILVSYYTIISKYCRNRAVTTAAMEMDDEENLAHIRHENQHQAPPA-GLDEALIK 125
           IL SA ILVSYYT+ISKYC     T+++  ++   N        Q  +    GL+E++IK
Sbjct: 64  ILTSALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFSSTQRISTNGDGLNESMIK 123

Query: 126 SITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCSHAFHLPCIDTWLKSHSTCPLC 185
           SITV KYK GDG V+G+DCSVCLS+F+ENESLRLLPKC+HAFHLPCIDTWLKSHS CPLC
Sbjct: 124 SITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLC 183

Query: 186 RSIIAPTNLFPPPTQEIQR------------------TEHLNASALQYQHRSND-TVVVV 245
           R+ +   N    PT  + +                     +N +   Y+ ++ D   VVV
Sbjct: 184 RAFVTGVN---NPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDFDSVVV 243

Query: 246 VQDLQGLTMRQETAVLGLENDDASFKNQRQSFASDSQDSEPRDRMIQVTQDDVVQPFRRS 305
           ++DL+          +G  N DA            S+   P +R  + T+D+   P RRS
Sbjct: 244 IEDLE----------IGSRNSDAR-----------SELQLPEER--RETKDEDSLPIRRS 303

Query: 306 VSLDSFSWQGQVSVADILRASQDSEEEVEEDELQMGIGSSKVFVQEH---------CQSN 363
           VSL+S      VS+AD+LR  +D E E        G+G+S+   +            ++N
Sbjct: 304 VSLNS---GVVVSIADVLREIEDEEGE------SGGVGTSQRREEGEDGDGKTIPPTEAN 357

BLAST of Sgr014951 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 255.4 bits (651), Expect = 1.0e-66
Identity = 163/377 (43.24%), Postives = 213/377 (56.50%), Query Frame = 0

Query: 1   MGSVGNSNQWQPY--AYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGD-SGTDFSPLI 60
           MGS GN N W     +Y+DCS  +CS+YCPQWCY+IFPPPP F   +  D S +DFSPL+
Sbjct: 1   MGSTGNPNPWGTTYDSYRDCSQGVCSVYCPQWCYVIFPPPPSFYLDDEDDSSSSDFSPLL 60

Query: 61  IAIIGILASAFILVSYYTIISKYCRNR-----AVTTAAMEMDDEENLAHIRHENQHQAPP 120
           IA+IGILASAFILVSYYT+ISKYC  R     + + AA+     +      + N +    
Sbjct: 61  IALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGAT 120

Query: 121 -----------AGLDEALIKSITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCSH 180
                       GLDE+LIKSITV KY++ DG VE +DCSVCLS+FQENESLRLLPKC+H
Sbjct: 121 NPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNH 180

Query: 181 AFHLPCIDTWLKSHSTCPLCRSIIAPTNLFPPPTQEIQRTEHLNASALQYQHRSNDTVVV 240
           AFH+PCIDTWLKSHS CPLCR+ I  ++      + +  T     +        +D+VVV
Sbjct: 181 AFHVPCIDTWLKSHSNCPLCRAFIVTSS----AVEIVDLTNQQIVTENNSISTGDDSVVV 240

Query: 241 VVQDLQGLTMRQETAVLGLENDDASFKNQRQSFASDSQDSEPRDRMIQVTQDDVVQPFRR 300
           V  DL+    R ET      N+ ++ K        DS+D E                 RR
Sbjct: 241 VNLDLENSRSRNETV-----NEGSTPKPPEM---QDSRDGEE----------------RR 300

Query: 301 SVSLDSFSWQGQVSVADILRASQDSEEEVEEDELQMGIGSSKVFVQE----------HCQ 349
           S SL+S    G VS+ADILR       E+E+DE   G+G+S+ +V+E             
Sbjct: 301 SASLNSGG--GVVSIADILR-------EIEDDEESAGVGTSR-WVEEGEGEKTPPPSGSA 339

BLAST of Sgr014951 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 224.6 bits (571), Expect = 1.9e-57
Identity = 139/337 (41.25%), Postives = 180/337 (53.41%), Query Frame = 0

Query: 10  WQPY-AYKDCSLEICSIYCPQWCYIIFPPPPPF---GYGNNGDSGTDFSPLIIAIIGILA 69
           W PY   +DCS  +CS+YCPQWCY IFPPPPPF   G   +  SG  FSPL+IAIIG+LA
Sbjct: 8   WVPYEPTRDCSQGLCSMYCPQWCYFIFPPPPPFDVAGTSADDSSGPVFSPLVIAIIGVLA 67

Query: 70  SAFILVSYYTIISKYCRNRAVTTAAMEMDDEENLAH---------------IRHENQHQA 129
           SAF+LVSYYT ISKYC   +     +        A+                 HE+ + +
Sbjct: 68  SAFLLVSYYTFISKYCGTVSSLRGRVFGSSSGGAAYGGGAGSGGRHGHGQSRSHESWNVS 127

Query: 130 PPAGLDEALIKSITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCSHAFHLPCIDT 189
           PP+GLDE LI  ITVCKY+RGDG V  TDCSVCL +F + ESLRLLP+CSHAFH  CIDT
Sbjct: 128 PPSGLDETLINKITVCKYRRGDGFVHTTDCSVCLGEFSDGESLRLLPRCSHAFHQQCIDT 187

Query: 190 WLKSHSTCPLCRSIIAPTNLFPPPTQEIQRTEHLNASALQYQHRSNDTVVVVVQDLQGLT 249
           WLKSHS CPLCR+ I         T  +   E    +  +    +   VVVV+  L+ L 
Sbjct: 188 WLKSHSNCPLCRANITFV------TVGLASPEPEGCAPGETGGDNTHEVVVVMDGLENLC 247

Query: 250 MRQETAV--LGLENDDASFKNQRQSFASDSQDSEPRDRMIQVTQDDVVQPFRRSVSLDSF 309
             Q+ AV      +DD   K+  +     +  +E R+            P +R  S    
Sbjct: 248 EEQQEAVSRASTADDDHDAKDVAEGMEEANGAAEIREE---------GSPPKRGASSFDL 307

Query: 310 SWQGQVSVADILRASQDSEEEV--EEDELQMGIGSSK 324
               ++ +AD+L+ S + E     E   L  G G+S+
Sbjct: 308 HRDNRMCIADVLQESMEDELTAARESGLLAGGAGTSR 329

BLAST of Sgr014951 vs. ExPASy Swiss-Prot
Match: P0C041 (Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 PE=3 SV=2)

HSP 1 Score: 178.3 bits (451), Expect = 1.5e-43
Identity = 98/208 (47.12%), Postives = 123/208 (59.13%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAY-KDCSLEICSIYCPQWCYIIFPPPPPFGYG---NNG-DSGTDFSP 60
           M S  N + W  Y   +DC+  +CS +CPQWC  I   PPP  Y    N+G  S  + SP
Sbjct: 1   MESDPNPSAWNQYINPRDCTQGLCSTFCPQWCTYINFSPPPISYEQFLNDGVASNPNLSP 60

Query: 61  LIIAIIGILASAFILVSYYTIISKYCRNRAVTTAAMEMDDEENLAHIRH-ENQHQAPP-- 120
           L+IAI GI A+AF+L +YYT++SKYC N     AA E    + +  +   E   Q  P  
Sbjct: 61  LVIAIFGIFATAFLLAAYYTLVSKYCANDTTNEAASESGRSDIILDVNSPERGDQDDPFA 120

Query: 121 -----AGLDEALIKSITVCKYKRGDG--LVEGTDCSVCLSDFQENESLRLLPKCSHAFHL 180
                AGLD+ LIK I   K K+      + GTDCS+CL +F E+ESLRLLPKC+H FH+
Sbjct: 121 LESSTAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSICLGEFNEDESLRLLPKCNHTFHV 180

Query: 181 PCIDTWLKSHSTCPLCRS-IIAPTNLFP 193
            CID WLKSHS CPLCR+ II PT   P
Sbjct: 181 VCIDRWLKSHSNCPLCRAKIIVPTTQQP 208

BLAST of Sgr014951 vs. ExPASy Swiss-Prot
Match: Q8LFY8 (RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2)

HSP 1 Score: 128.6 bits (322), Expect = 1.4e-28
Identity = 118/384 (30.73%), Postives = 177/384 (46.09%), Query Frame = 0

Query: 17  DCSLEICSIYCPQWC------YIIFPPPPPFG----------------YGNNGDSGTDFS 76
           DCS  +C   CP  C      Y I P  PP+                 Y  + DS +   
Sbjct: 22  DCSNGVCDPICPYNCYPEPDYYTISPQLPPWSSSPQPSPCPSPSISAVYLPSQDSSSSLD 81

Query: 77  PLII-----AIIGILASAFILVSYYTIISKYCRNRAVTTAAMEMDDEENLAHIRHENQHQ 136
            + I     A++ IL + F LV+ +   S    NR V     + D+E+N   +  E Q +
Sbjct: 82  AISIITITGAVLAILLTGFFLVAKFFSDSV---NR-VNQGTYQSDNEDNDTVMEEEFQDR 141

Query: 137 AP---------PAGLDEALIKSITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCS 196
                        GL +++I SIT+C YKRGDGL+E TDC VCL++F+E+ESLRLLPKC+
Sbjct: 142 EQVDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKCN 201

Query: 197 HAFHLPCIDTWLKSHSTCPLCRSIIAPTNLFPPP-TQEIQRTEHLNASALQYQHRSNDTV 256
           HAFH+ CIDTWL SH+ CPLCR+ IA  ++  P  +  ++ T   + S     H  ND  
Sbjct: 202 HAFHISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVEVTPGGSGS-----HLENDG- 261

Query: 257 VVVVQDLQGLTMRQETAVLGLENDDASFKNQRQSFASDSQDSEPRDRMIQVTQDDVVQPF 316
            V  +D   +  R ++     E+DD+  + +   F SD   SE   +       + + P 
Sbjct: 262 -VDEEDHGEIENRVDSDF--KESDDSDIRIEIYRFDSDGDGSETETKERVRVLKECMDP- 321

Query: 317 RRSVSLDSFSWQGQVSVADILRASQDSEEEVEEDELQMGIGSSKVFVQEHCQSNYKTGVS 363
               S++S S   +  V  +    +  E + EE     G        ++    + + G  
Sbjct: 322 NGGDSVNSLS-HTKTHVESVDFPGKSCENQSEEFTRHNG--------EDEASCSEENGGG 381

BLAST of Sgr014951 vs. ExPASy TrEMBL
Match: A0A6J1D151 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111016068 PE=3 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 4.3e-190
Identity = 340/373 (91.15%), Postives = 351/373 (94.10%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRAVTT-AAMEMDDEENLAHIRHENQHQAPPAGLDEAL 120
           IGILASAFILVSYYTIISKYCRNRA TT AAMEMDDEENLA IRHENQ QAPPAGLDEAL
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRAATTSAAMEMDDEENLAQIRHENQLQAPPAGLDEAL 120

Query: 121 IKSITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCSHAFHLPCIDTWLKSHSTCP 180
           IKSITVCKY RGDGLVEGTDCSVCLS+FQENESLRLLPKCSHAFHLPCIDTWLKSHSTCP
Sbjct: 121 IKSITVCKYIRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHSTCP 180

Query: 181 LCRSIIAPTNLFPPPTQEIQRTEHLNASALQYQHRSNDT-VVVVVQDLQGLTMRQETAVL 240
           LCRS I+PTNLFPPPTQEIQRTEH+NA+ALQYQHRSNDT VVVVVQDLQ LT+ Q+TAVL
Sbjct: 181 LCRSNISPTNLFPPPTQEIQRTEHINAAALQYQHRSNDTVVVVVVQDLQDLTVSQQTAVL 240

Query: 241 GLENDDASFKNQRQSFASDSQDSEPRDRMIQVTQD----DVVQPFRRSVSLDSFSWQGQV 300
           G+END AS KNQRQSFAS SQDSEPRDRMIQV +D    DVVQPFRRSVSLDSFSWQGQV
Sbjct: 241 GIENDGASSKNQRQSFASVSQDSEPRDRMIQVREDDVVGDVVQPFRRSVSLDSFSWQGQV 300

Query: 301 SVADILRASQDSEEEVEEDELQMGIGSSKVFVQEHCQSNYKTGVSNLGMNRSISTGRLGF 360
           SVADILR SQDSEEEVEEDE+QMGIGSSKVFVQEH QSN+K GVSNLGMNRS+STGRLGF
Sbjct: 301 SVADILRESQDSEEEVEEDEMQMGIGSSKVFVQEHFQSNHKNGVSNLGMNRSVSTGRLGF 360

Query: 361 TNYGKGKSCINPS 368
           TNY KGKSCI PS
Sbjct: 361 TNYEKGKSCIIPS 373

BLAST of Sgr014951 vs. ExPASy TrEMBL
Match: A0A6J1IR45 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111479735 PE=3 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 3.9e-175
Identity = 319/370 (86.22%), Postives = 333/370 (90.00%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAY DCSLEICSIYCPQWCYIIFPPPPPFGYG+NGDS TDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYNDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRAVTT-AAMEMDDEENLAHIRHENQHQA-PPAGLDEA 120
           IGILASAFI+VSYYTIISKYCRNRA TT AAMEMDDEENL HIRHENQ QA PPAGLD A
Sbjct: 61  IGILASAFIMVSYYTIISKYCRNRAATTNAAMEMDDEENLTHIRHENQLQAPPPAGLDHA 120

Query: 121 LIKSITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCSHAFHLPCIDTWLKSHSTC 180
           LIKSITVCKYKRGD LVEGTDCSVCLS+FQENESLRLLPKCSHAFHLPCIDTWLKSHSTC
Sbjct: 121 LIKSITVCKYKRGDDLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHSTC 180

Query: 181 PLCRSIIAPTNLFPPPTQEIQRTEHLNASALQYQHRSNDT-VVVVVQDLQGLTMRQETAV 240
           PLCRS I+PTN+ PPPTQEI RTEHLNASALQYQHR+NDT VVVVVQDLQ LT+RQETAV
Sbjct: 181 PLCRSNISPTNVSPPPTQEILRTEHLNASALQYQHRTNDTVVVVVVQDLQDLTVRQETAV 240

Query: 241 LGLENDDASFKNQRQSFASDSQDSEPRDRMIQVTQDDVVQPFRRSVSLDSFSWQGQVSVA 300
            GLENDDAS KN+RQSFASD QDSEPRDRM Q      VQP+RRSVSL+S SWQGQVSVA
Sbjct: 241 SGLENDDASSKNRRQSFASDCQDSEPRDRMNQ------VQPYRRSVSLNSLSWQGQVSVA 300

Query: 301 DILRASQDSEEEVEEDELQMGIGSSKVFVQEHCQSNYKTGVSNLGMNRSISTGRLGFTNY 360
           DILRASQDSE+EVEEDELQMGIGSSK+F QEHC         +LGMNRSISTG+ GFTNY
Sbjct: 301 DILRASQDSEDEVEEDELQMGIGSSKLFTQEHCH--------DLGMNRSISTGKQGFTNY 356

Query: 361 GKGKSCINPS 368
           GKGKSCI PS
Sbjct: 361 GKGKSCIIPS 356

BLAST of Sgr014951 vs. ExPASy TrEMBL
Match: A0A6J1IC58 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111473464 PE=3 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 1.6e-171
Identity = 319/374 (85.29%), Postives = 333/374 (89.04%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAY+DCSLEICSIYCPQWCYIIFPPPPPFGYG N DSGTDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYRDCSLEICSIYCPQWCYIIFPPPPPFGYGTNSDSGTDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRAVTT-AAMEMDDEENLAHIRHENQHQA--PPAGLDE 120
           IGILASAFILVSYYTIISKYCRNRA T+ + MEMDDEENL+ IRHENQ QA  PPAGLDE
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRAATSNSNMEMDDEENLSQIRHENQLQAPLPPAGLDE 120

Query: 121 ALIKSITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCSHAFHLPCIDTWLKSHST 180
           ALIKSITVCKYKRGDGLVEGTDCSVCLS+F+ENESLRLLPKCSHAFHLPCIDTWLKSHST
Sbjct: 121 ALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHST 180

Query: 181 CPLCRSIIAPTNLFPPPTQEIQRTEHLNASALQYQHRSNDT-VVVVVQDLQGLTM-RQET 240
           CPLCRS I+P NLFPPPTQEIQRTEHLN+   QYQHRSNDT VVVVVQDL  L +  QET
Sbjct: 181 CPLCRSNISPINLFPPPTQEIQRTEHLNSYNFQYQHRSNDTVVVVVVQDLPDLPVSSQET 240

Query: 241 AVLGLENDDASFKNQRQSFASDSQDSEPRDRMI-QVTQDDVVQPFRRSVSLDSFSWQGQV 300
           AVLGLEND  S KNQRQSF SD+QDSEPR+ MI QV QDD VQPFRRSVSL+S SWQGQV
Sbjct: 241 AVLGLENDGVSSKNQRQSFVSDNQDSEPRNIMIHQVRQDDAVQPFRRSVSLNSLSWQGQV 300

Query: 301 SVADILRASQDSEEEVEEDELQMGIGSSKVFVQEHCQSNYKTGVSNLGMNRSISTGRLGF 360
           SVADILRASQDSEEEVEEDELQMGIGSSK FVQE C S     VSNL M+RSISTG+LGF
Sbjct: 301 SVADILRASQDSEEEVEEDELQMGIGSSKAFVQEDCHSR----VSNLEMHRSISTGKLGF 360

Query: 361 TNYG--KGKSCINP 367
           TNYG  KGKSCI P
Sbjct: 361 TNYGNWKGKSCIIP 370

BLAST of Sgr014951 vs. ExPASy TrEMBL
Match: A0A6J1G2W9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111450329 PE=3 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 1.6e-171
Identity = 319/374 (85.29%), Postives = 332/374 (88.77%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAY+DCSLEICSIYCPQWCYIIFPPPPPFGYG N DSGTDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYRDCSLEICSIYCPQWCYIIFPPPPPFGYGANSDSGTDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRAVTT-AAMEMDDEENLAHIRHENQHQA--PPAGLDE 120
           IGILASAFILVSYYTIISKYCRNRA T+ + MEMDDEENL+ IRHENQ QA  PPAGLDE
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRATTSNSNMEMDDEENLSQIRHENQLQAPPPPAGLDE 120

Query: 121 ALIKSITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCSHAFHLPCIDTWLKSHST 180
           ALI SITVCKYKRGDGLVEGTDCSVCLS+F+ENESLRLLPKCSHAFHLPCIDTWLKSHST
Sbjct: 121 ALINSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHST 180

Query: 181 CPLCRSIIAPTNLFPPPTQEIQRTEHLNASALQYQHRSNDT-VVVVVQDLQGLTM-RQET 240
           CPLCRS I+P NLFPPPTQEIQRTEHLN+   QYQHRSNDT VVVVVQDL  LT+  QET
Sbjct: 181 CPLCRSNISPINLFPPPTQEIQRTEHLNSYNFQYQHRSNDTVVVVVVQDLPDLTVSSQET 240

Query: 241 AVLGLENDDASFKNQRQSFASDSQDSEPRDRMI-QVTQDDVVQPFRRSVSLDSFSWQGQV 300
           AVLGLEND  S KNQRQSF SD+QDSEPRD MI Q  QDD VQPFRRSVSL+S SWQGQV
Sbjct: 241 AVLGLENDGVSSKNQRQSFVSDNQDSEPRDIMIHQTRQDDAVQPFRRSVSLNSLSWQGQV 300

Query: 301 SVADILRASQDSEEEVEEDELQMGIGSSKVFVQEHCQSNYKTGVSNLGMNRSISTGRLGF 360
           SVADILRASQDSEEEVEEDELQMGIGSSK FVQE C S     VSNL M+RSISTG+LGF
Sbjct: 301 SVADILRASQDSEEEVEEDELQMGIGSSKAFVQEDCHSR----VSNLEMHRSISTGKLGF 360

Query: 361 TNYG--KGKSCINP 367
           TNYG  KGKSCI P
Sbjct: 361 TNYGNWKGKSCIIP 370

BLAST of Sgr014951 vs. ExPASy TrEMBL
Match: A0A5D3D2D1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003080 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 4.4e-166
Identity = 314/381 (82.41%), Postives = 334/381 (87.66%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYG+NGDS TDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRAVTT-AAMEMDDEENLAHIRHENQHQA---PPAGLD 120
           IGILASAFILVSYYTIISKYCRNRA T+  AMEM+DEENL+ IRHENQ QA   PP GLD
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENLSQIRHENQLQAPPLPPPGLD 120

Query: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCSHAFHLPCIDTWLKSHS 180
           EALIKSITVCKYKRGDGLVEGTDCSVCLS+F+ENESLRLLPKCSHAFHLPCIDTWLKSHS
Sbjct: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS 180

Query: 181 TCPLCRSIIAPTNLFPPPTQEIQRTEHLNASALQY--QHRSNDTV-VVVVQDLQGLT-MR 240
           TCPLCRS I+PTNLF   TQEIQ T+H  +SA QY  QHR+NDTV VVVVQDL  LT +R
Sbjct: 181 TCPLCRSNISPTNLFSTATQEIQ-TQHFVSSAFQYQHQHRTNDTVLVVVVQDLDDLTVVR 240

Query: 241 QETAVLGLENDDASFKNQRQSFASDSQDSEPRDRMIQVTQ-----DDVVQPFRRSVSLDS 300
           QET V  LENDDAS KNQR+ +AS+SQ+S  R+RM QV Q     D VVQPFRRSVSL+S
Sbjct: 241 QETVVSRLENDDASSKNQREGYASESQNSGERERMNQVRQEYDVVDGVVQPFRRSVSLNS 300

Query: 301 FSWQGQVSVADILRASQDSEEEVEEDEL-QMGIGSSKVFVQEHCQSNYKTGVSNLGMNRS 360
            SWQG VSVADILR SQDSEEEVEEDEL QMGIGSSKVFVQE   SN++TGVSNLGMNRS
Sbjct: 301 LSWQGPVSVADILRVSQDSEEEVEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNRS 360

Query: 361 ISTGRLGFTNYGKGKSCINPS 368
           ISTG+LGFTNYGKGKSCI PS
Sbjct: 361 ISTGKLGFTNYGKGKSCIIPS 380

BLAST of Sgr014951 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 258.5 bits (659), Expect = 8.4e-69
Identity = 163/389 (41.90%), Postives = 222/389 (57.07%), Query Frame = 0

Query: 6   NSNQWQPY-AYKDCSLEICSIYCPQWCYIIFPPPPP--FGYGNNGDSGTDFSPLIIAIIG 65
           N N W PY +Y DCS  IC+IYCPQWCY+IFPPPPP  F   ++  S + FSPL+IA+IG
Sbjct: 4   NPNPWSPYDSYNDCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALIG 63

Query: 66  ILASAFILVSYYTIISKYCRNRAVTTAAMEMDDEENLAHIRHENQHQAPPA-GLDEALIK 125
           IL SA ILVSYYT+ISKYC     T+++  ++   N        Q  +    GL+E++IK
Sbjct: 64  ILTSALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFSSTQRISTNGDGLNESMIK 123

Query: 126 SITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCSHAFHLPCIDTWLKSHSTCPLC 185
           SITV KYK GDG V+G+DCSVCLS+F+ENESLRLLPKC+HAFHLPCIDTWLKSHS CPLC
Sbjct: 124 SITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLC 183

Query: 186 RSIIAPTNLFPPPTQEIQR------------------TEHLNASALQYQHRSND-TVVVV 245
           R+ +   N    PT  + +                     +N +   Y+ ++ D   VVV
Sbjct: 184 RAFVTGVN---NPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDFDSVVV 243

Query: 246 VQDLQGLTMRQETAVLGLENDDASFKNQRQSFASDSQDSEPRDRMIQVTQDDVVQPFRRS 305
           ++DL+          +G  N DA            S+   P +R  + T+D+   P RRS
Sbjct: 244 IEDLE----------IGSRNSDAR-----------SELQLPEER--RETKDEDSLPIRRS 303

Query: 306 VSLDSFSWQGQVSVADILRASQDSEEEVEEDELQMGIGSSKVFVQEH---------CQSN 363
           VSL+S      VS+AD+LR  +D E E        G+G+S+   +            ++N
Sbjct: 304 VSLNS---GVVVSIADVLREIEDEEGE------SGGVGTSQRREEGEDGDGKTIPPTEAN 357

BLAST of Sgr014951 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 255.4 bits (651), Expect = 7.1e-68
Identity = 163/377 (43.24%), Postives = 213/377 (56.50%), Query Frame = 0

Query: 1   MGSVGNSNQWQPY--AYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGD-SGTDFSPLI 60
           MGS GN N W     +Y+DCS  +CS+YCPQWCY+IFPPPP F   +  D S +DFSPL+
Sbjct: 1   MGSTGNPNPWGTTYDSYRDCSQGVCSVYCPQWCYVIFPPPPSFYLDDEDDSSSSDFSPLL 60

Query: 61  IAIIGILASAFILVSYYTIISKYCRNR-----AVTTAAMEMDDEENLAHIRHENQHQAPP 120
           IA+IGILASAFILVSYYT+ISKYC  R     + + AA+     +      + N +    
Sbjct: 61  IALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGAT 120

Query: 121 -----------AGLDEALIKSITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCSH 180
                       GLDE+LIKSITV KY++ DG VE +DCSVCLS+FQENESLRLLPKC+H
Sbjct: 121 NPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNH 180

Query: 181 AFHLPCIDTWLKSHSTCPLCRSIIAPTNLFPPPTQEIQRTEHLNASALQYQHRSNDTVVV 240
           AFH+PCIDTWLKSHS CPLCR+ I  ++      + +  T     +        +D+VVV
Sbjct: 181 AFHVPCIDTWLKSHSNCPLCRAFIVTSS----AVEIVDLTNQQIVTENNSISTGDDSVVV 240

Query: 241 VVQDLQGLTMRQETAVLGLENDDASFKNQRQSFASDSQDSEPRDRMIQVTQDDVVQPFRR 300
           V  DL+    R ET      N+ ++ K        DS+D E                 RR
Sbjct: 241 VNLDLENSRSRNETV-----NEGSTPKPPEM---QDSRDGEE----------------RR 300

Query: 301 SVSLDSFSWQGQVSVADILRASQDSEEEVEEDELQMGIGSSKVFVQE----------HCQ 349
           S SL+S    G VS+ADILR       E+E+DE   G+G+S+ +V+E             
Sbjct: 301 SASLNSGG--GVVSIADILR-------EIEDDEESAGVGTSR-WVEEGEGEKTPPPSGSA 339

BLAST of Sgr014951 vs. TAIR 10
Match: AT4G17905.1 (RING/U-box superfamily protein )

HSP 1 Score: 178.3 bits (451), Expect = 1.1e-44
Identity = 98/208 (47.12%), Postives = 123/208 (59.13%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAY-KDCSLEICSIYCPQWCYIIFPPPPPFGYG---NNG-DSGTDFSP 60
           M S  N + W  Y   +DC+  +CS +CPQWC  I   PPP  Y    N+G  S  + SP
Sbjct: 1   MESDPNPSAWNQYINPRDCTQGLCSTFCPQWCTYINFSPPPISYEQFLNDGVASNPNLSP 60

Query: 61  LIIAIIGILASAFILVSYYTIISKYCRNRAVTTAAMEMDDEENLAHIRH-ENQHQAPP-- 120
           L+IAI GI A+AF+L +YYT++SKYC N     AA E    + +  +   E   Q  P  
Sbjct: 61  LVIAIFGIFATAFLLAAYYTLVSKYCANDTTNEAASESGRSDIILDVNSPERGDQDDPFA 120

Query: 121 -----AGLDEALIKSITVCKYKRGDG--LVEGTDCSVCLSDFQENESLRLLPKCSHAFHL 180
                AGLD+ LIK I   K K+      + GTDCS+CL +F E+ESLRLLPKC+H FH+
Sbjct: 121 LESSTAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSICLGEFNEDESLRLLPKCNHTFHV 180

Query: 181 PCIDTWLKSHSTCPLCRS-IIAPTNLFP 193
            CID WLKSHS CPLCR+ II PT   P
Sbjct: 181 VCIDRWLKSHSNCPLCRAKIIVPTTQQP 208

BLAST of Sgr014951 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 129.0 bits (323), Expect = 7.7e-30
Identity = 74/185 (40.00%), Postives = 104/185 (56.22%), Query Frame = 0

Query: 29  QWCYIIFPPPPPFGYGNNGDSGTDFS---PLIIAIIG-ILASAFILVSYYTIISKYCRNR 88
           ++C+  +PPPPP           + S   P  I ++G I+   F++   Y  I++  R  
Sbjct: 19  RFCFRQYPPPPP-----PPPPPRELSLLLPTSICVVGSIILFLFLVFFLYLHITQQRRIS 78

Query: 89  AVTTA---AMEMDDEENLAHIRHENQH---QAPPAGLDEALIKSITVCKYKRGDGLVEGT 148
           A +       + +DE+        + H   Q P  GL  + I SITV  +K+G+G+++GT
Sbjct: 79  AASVTPGDTNQQEDEDETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGEGIIDGT 138

Query: 149 DCSVCLSDFQENESLRLLPKCSHAFHLPCIDTWLKSHSTCPLCRSIIAPTNLFPPPTQEI 204
           +CSVCL++F+E+ESLRLLPKCSHAFHL CIDTWL SH  CPLCR   AP  L   P  + 
Sbjct: 139 ECSVCLNEFEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCR---APVLLITEPPHQE 195

BLAST of Sgr014951 vs. TAIR 10
Match: AT1G72220.1 (RING/U-box superfamily protein )

HSP 1 Score: 128.6 bits (322), Expect = 1.0e-29
Identity = 118/384 (30.73%), Postives = 177/384 (46.09%), Query Frame = 0

Query: 17  DCSLEICSIYCPQWC------YIIFPPPPPFG----------------YGNNGDSGTDFS 76
           DCS  +C   CP  C      Y I P  PP+                 Y  + DS +   
Sbjct: 22  DCSNGVCDPICPYNCYPEPDYYTISPQLPPWSSSPQPSPCPSPSISAVYLPSQDSSSSLD 81

Query: 77  PLII-----AIIGILASAFILVSYYTIISKYCRNRAVTTAAMEMDDEENLAHIRHENQHQ 136
            + I     A++ IL + F LV+ +   S    NR V     + D+E+N   +  E Q +
Sbjct: 82  AISIITITGAVLAILLTGFFLVAKFFSDSV---NR-VNQGTYQSDNEDNDTVMEEEFQDR 141

Query: 137 AP---------PAGLDEALIKSITVCKYKRGDGLVEGTDCSVCLSDFQENESLRLLPKCS 196
                        GL +++I SIT+C YKRGDGL+E TDC VCL++F+E+ESLRLLPKC+
Sbjct: 142 EQVDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKCN 201

Query: 197 HAFHLPCIDTWLKSHSTCPLCRSIIAPTNLFPPP-TQEIQRTEHLNASALQYQHRSNDTV 256
           HAFH+ CIDTWL SH+ CPLCR+ IA  ++  P  +  ++ T   + S     H  ND  
Sbjct: 202 HAFHISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVEVTPGGSGS-----HLENDG- 261

Query: 257 VVVVQDLQGLTMRQETAVLGLENDDASFKNQRQSFASDSQDSEPRDRMIQVTQDDVVQPF 316
            V  +D   +  R ++     E+DD+  + +   F SD   SE   +       + + P 
Sbjct: 262 -VDEEDHGEIENRVDSDF--KESDDSDIRIEIYRFDSDGDGSETETKERVRVLKECMDP- 321

Query: 317 RRSVSLDSFSWQGQVSVADILRASQDSEEEVEEDELQMGIGSSKVFVQEHCQSNYKTGVS 363
               S++S S   +  V  +    +  E + EE     G        ++    + + G  
Sbjct: 322 NGGDSVNSLS-HTKTHVESVDFPGKSCENQSEEFTRHNG--------EDEASCSEENGGG 381

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147036.19.0e-19091.15RING-H2 finger protein ATL52-like [Momordica charantia][more]
XP_022980342.18.1e-17586.22RING-H2 finger protein ATL52-like [Cucurbita maxima][more]
XP_023540121.17.6e-17385.60RING-H2 finger protein ATL51-like [Cucurbita pepo subsp. pepo][more]
KAG7028733.13.8e-17285.56RING-H2 finger protein ATL51, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
KAG6597262.14.9e-17285.56RING-H2 finger protein ATL51, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9LF641.2e-6741.90RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1[more]
Q9SRQ81.0e-6643.24RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2[more]
Q7XLY81.9e-5741.25E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994... [more]
P0C0411.5e-4347.12Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 P... [more]
Q8LFY81.4e-2830.73RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1D1514.3e-19091.15RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101606... [more]
A0A6J1IR453.9e-17586.22RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111479735 P... [more]
A0A6J1IC581.6e-17185.29RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111473464 P... [more]
A0A6J1G2W91.6e-17185.29RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111450329... [more]
A0A5D3D2D14.4e-16682.41RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
AT5G17600.18.4e-6941.90RING/U-box superfamily protein [more]
AT3G03550.17.1e-6843.24RING/U-box superfamily protein [more]
AT4G17905.11.1e-4447.12RING/U-box superfamily protein [more]
AT5G10380.17.7e-3040.00RING/U-box superfamily protein [more]
AT1G72220.11.0e-2930.73RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 140..181
e-value: 1.8E-7
score: 40.8
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 139..182
e-value: 1.6E-13
score: 50.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 140..182
score: 12.887609
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 99..187
e-value: 2.3E-21
score: 77.2
IPR044675E3 ubiquitin-protein ligase RING1-likePANTHERPTHR45676RING-H2 FINGER PROTEIN ATL51-RELATEDcoord: 3..357
NoneNo IPR availablePANTHERPTHR45676:SF95RING-H2 FINGER PROTEIN ATL53-RELATEDcoord: 3..357
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 139..182
e-value: 2.26879E-18
score: 75.8013
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 128..189

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr014951.1Sgr014951.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane