Sgr001405 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr001405
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionPyruvate kinase family protein
Locationtig00000892: 95419 .. 98514 (-)
RNA-Seq ExpressionSgr001405
SyntenySgr001405
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGGATTACTCATTGAGAAGAAACCCAAGACTAAAATAGTCTGCACCTTGGGCCCTGCATCCAGGTCCGTCCCCATGATCGAAAAGCTTTTGAAGGCTGGGATGAATGTTGCTCGTTTCAACTTCTCCCACGGATCCCATGCCTATCACCAGGAGACCCTCGACAATCTCAGGTCCGCGATGGAGAACACCGGCATTCTCTGCGCGGTCATGCTTGACACCAAGGTTTCTCTCATCCTACTTTTCTTTCCTCACCATCTCTAGTTGACCAAACGCTCGATTTCTTGGTTTTGGTTTGTACATATCTACTTGCCTCTTTTGACTTATTTTTTGTTCTTATCGTCTTCTTTTGGTTTTATTTTCTTAGACTCACAGACATCTTGGCCTTTTGAGTTTTAGATGTTTCCAGAGAATCTAATTGGTTTCTTCTCCCTCTTGCATGATAAGTCGTGTTTCCTTGTGCACAGTTTCCCACAAAATGTACTCCTTTTGTCGGTTACTTCAGCTACCAGTGCTTATGCGTAGCCGCACATATTTTTAAGCATCTCTTTCTCTTGTGGAGAAGAATATAGACTCGGAAGAGAAGTAGATGAATAGACTTTTAATATACTTAGGAAAAGGAAGATGAATAATAGACTTTAGCATACTTGTAAAGTTTTCTTTTTCAAAAGAAGGACTAAGGTGAAGCAAATTACAAATTAACCCTTATCCATCTTGCAACACATTCACGACAAGCCGATTACATTCAACCAGAAAAGAGCGAGTTCCTGCAAGCTGCAACACATTCACATGGAGGTGCTTCCAGGTTCGAATGGGAGATAAAGGGGTTTTGTGCTTACAAGGTTGAATGGAAAAAAGTGGGGGAGGGGTTTTGTGCTTGCAGGAATCTGGGCGAGATGTGTTTTTTTAGTTTTATTTTCTACAAGGTGAAAGAAGAAGGCGATGTATTTTTTACTGAATACGAGTGATTGTAGGTTTTTTGATGTATTTTTTCAACCGTTTAGCCATTATGATGGTACATCTTCACTAACTGCTCGTTTGTTTTGTCTGGATATTTGTTACTTTCTAGTTGCCTTGTCTTTGAACATGACCAAGTTTCTTTTTTCACATTTGTATTCTGTTGAGGGTAAATCAGTATTCTTGAATAGTTCGAGGCAGCACTTGAACTGTGAGAAATAAATTTTGAGGTTAATTCCAGTTATATTCACCTCATTGCTGCTCCTGTTTAGTTTTATTAGCTTTCTTGAGAGCCAGGAACAATTGCCCTTTATGATTTCTGTTTCATATCTCTTATTGATGTTCAGCTTGTAGGAAATTCTTTTTGTGACGTACCATGCCATTCTTATTTTTCTTTCTATGTAAAACTACTACATTTGAAGTTTATAATATATTAGTTAATTATTGATGTCCATGGAATCTTGAGGATACACAAATGCCACATATATTACTTGGAGATGCCCCATCCATAACTTCACCTTTCCGTTTAAAGCAATGCATGAATGCAATTTGTTTTGCATTAATAAAATTTAATGATTCTTGACTTCAAAATGAAACAGGGCCCAGAGATTCGTACTGGATTTTTGAAAGATAGTAAACCCATCCAGCTAAAACAGGGGCAGGAGATAACTATATCTACCGACTACAGCTTGAAGGGTGATGAGAATATGATATGTATGAGCTACAAAAAGTTAGCTGAGGATGTAAAGCCAGGGAGCATAATACTTTGTGCAGATGGTACGATCTCATTTACAGTTCTATCTTGTGACAAACAATTGGGTTTGGTACATGTCGCTGTGAGAACTCTGCAGTTCTTGGTGAAAGAAAAAATGTTAACCTGCCTGGAGTTATAGTGGATCTCCCAACCCTGACAGACAAAGATAAGGAAGATATACTAGAATGGGGGATTCCTAATAAAATTGACATGATTGCTCTATCTTTTGTTCGGAAAGGTTCTGATCTTGTGGAGGTCCGAAAGTTGCTTGGAAAGCATGCCAAGAGTATTCTTCTCATGTCAAAGGTACATTCTGTTTCATGGAAATTTCCTGTGAAAATATGTTCTATTCATGAGGGTGATAACAGTAAGAAACTTTTTTTAATTTAATTTCTGGGAATTCAGCCTTTTCTGGTGATTTGTGGCACGGGCACTAGGAAATGTTAAAATGCACTTTAGAAGAAAATCATGCAGCACAAATGTTTTGAGATCGCTTTTTCTATGTATCCTAATATCTCTGTACTAAAAGCAATTTCGTACCTGTTATTTTTTTGGAATTTGATTCACAGGTTGAAAATCAAGAAGGTGTGGCAAATTTTGATGACATACTAGCCAATTCAGATGCATTCATGGTGGCACGTGGTGACCTGGGAATGGAAATTCCAATCGAGAAGATATTTCTGGCGCAGAAAGTGATGATATACAAGTGCAACATCCAGGGAAAACCTGTTGTCACAGCCACACAGATGCTGGAATCAATGATTAAATCTCCAAGACCAACTAGAGCCGAAGCCACTGACGTTGCTAATGCCGTTCTTGATGGGACAGATTGCGTAATGTTAAGTGGTGAAACAGCTGCTGGAGCGTATCCAGAACTTGCTGTTAGAACAATGGCAAAAATATGTGTTGAAGCTGAAAGCACCCTTGATTATGGAGATGTCTTTAAAAGGATTATGGAACATTCCCCAGTTCCCATGAGCCCATTAGAAAGCCTAGCTTCTTCTGCTGTTAGAACTGCAAACTCAGCCAAGGCAGCTCTTATACTTGTCCTAACCAGAGGAGGAAGCACTGCAAAACTGGTGGCCAAGTATAGGCCAGGAACGCCCATCTTGTCTGTGGTTGTCCCCGAGATTAAGACAGATTCATTTGATTGGTCATGCAGCGATGAAGCTCCGGCAAGGCATAGCTTAATTTTTCGTGGGTTGATACCGGTTCTTAGCACAGCATCGGCAAGGGCGTCTCATGCAGAGACAACAGAAGAAGCCATAGAGTTCGCTATTCAGCATGCGAAGTCGAAGGGGCTCTGCAAAAATGGAGATTCTGTCGTGGCTCTTCATCGCGTTGGAACAGCATCTGTTATCAAGATATTGACTGTGAAGTAG

mRNA sequence

ATGGACGGATTACTCATTGAGAAGAAACCCAAGACTAAAATAGTCTGCACCTTGGGCCCTGCATCCAGGTCCGTCCCCATGATCGAAAAGCTTTTGAAGGCTGGGATGAATGTTGCTCGTTTCAACTTCTCCCACGGATCCCATGCCTATCACCAGGAGACCCTCGACAATCTCAGGTCCGCGATGGAGAACACCGGCATTCTCTGCGCGGTCATGCTTGACACCAAGGGCCCAGAGATTCGTACTGGATTTTTGAAAGATAGTAAACCCATCCAGCTAAAACAGGGGCAGGAGATAACTATATCTACCGACTACAGCTTGAAGGGTGATGAGAATATGATATGTATGAGCTACAAAAAGTTAGCTGAGGATGTAAAGCCAGGGAGCATAATACTTTGTGCAGATGTTCTTGGTGAAAGAAAAAATGTTAACCTGCCTGGAGTTATAGTGGATCTCCCAACCCTGACAGACAAAGATAAGGAAGATATACTAGAATGGGGGATTCCTAATAAAATTGACATGATTGCTCTATCTTTTGTTCGGAAAGGTTCTGATCTTGTGGAGGTCCGAAAGTTGCTTGGAAAGCATGCCAAGAGTATTCTTCTCATGTCAAAGGTTGAAAATCAAGAAGGTGTGGCAAATTTTGATGACATACTAGCCAATTCAGATGCATTCATGGTGGCACGTGGTGACCTGGGAATGGAAATTCCAATCGAGAAGATATTTCTGGCGCAGAAAGTGATGATATACAAGTGCAACATCCAGGGAAAACCTGTTGTCACAGCCACACAGATGCTGGAATCAATGATTAAATCTCCAAGACCAACTAGAGCCGAAGCCACTGACGTTGCTAATGCCGTTCTTGATGGGACAGATTGCGTAATGTTAAGTGGTGAAACAGCTGCTGGAGCGTATCCAGAACTTGCTGTTAGAACAATGGCAAAAATATGTGTTGAAGCTGAAAGCACCCTTGATTATGGAGATGTCTTTAAAAGGATTATGGAACATTCCCCAGTTCCCATGAGCCCATTAGAAAGCCTAGCTTCTTCTGCTGTTAGAACTGCAAACTCAGCCAAGGCAGCTCTTATACTTGTCCTAACCAGAGGAGGAAGCACTGCAAAACTGGTGGCCAAGTATAGGCCAGGAACGCCCATCTTGTCTGTGGTTGTCCCCGAGATTAAGACAGATTCATTTGATTGGTCATGCAGCGATGAAGCTCCGGCAAGGCATAGCTTAATTTTTCGTGGGTTGATACCGGTTCTTAGCACAGCATCGGCAAGGGCGTCTCATGCAGAGACAACAGAAGAAGCCATAGAGTTCGCTATTCAGCATGCGAAGTCGAAGGGGCTCTGCAAAAATGGAGATTCTGTCGTGGCTCTTCATCGCGTTGGAACAGCATCTGTTATCAAGATATTGACTGTGAAGTAG

Coding sequence (CDS)

ATGGACGGATTACTCATTGAGAAGAAACCCAAGACTAAAATAGTCTGCACCTTGGGCCCTGCATCCAGGTCCGTCCCCATGATCGAAAAGCTTTTGAAGGCTGGGATGAATGTTGCTCGTTTCAACTTCTCCCACGGATCCCATGCCTATCACCAGGAGACCCTCGACAATCTCAGGTCCGCGATGGAGAACACCGGCATTCTCTGCGCGGTCATGCTTGACACCAAGGGCCCAGAGATTCGTACTGGATTTTTGAAAGATAGTAAACCCATCCAGCTAAAACAGGGGCAGGAGATAACTATATCTACCGACTACAGCTTGAAGGGTGATGAGAATATGATATGTATGAGCTACAAAAAGTTAGCTGAGGATGTAAAGCCAGGGAGCATAATACTTTGTGCAGATGTTCTTGGTGAAAGAAAAAATGTTAACCTGCCTGGAGTTATAGTGGATCTCCCAACCCTGACAGACAAAGATAAGGAAGATATACTAGAATGGGGGATTCCTAATAAAATTGACATGATTGCTCTATCTTTTGTTCGGAAAGGTTCTGATCTTGTGGAGGTCCGAAAGTTGCTTGGAAAGCATGCCAAGAGTATTCTTCTCATGTCAAAGGTTGAAAATCAAGAAGGTGTGGCAAATTTTGATGACATACTAGCCAATTCAGATGCATTCATGGTGGCACGTGGTGACCTGGGAATGGAAATTCCAATCGAGAAGATATTTCTGGCGCAGAAAGTGATGATATACAAGTGCAACATCCAGGGAAAACCTGTTGTCACAGCCACACAGATGCTGGAATCAATGATTAAATCTCCAAGACCAACTAGAGCCGAAGCCACTGACGTTGCTAATGCCGTTCTTGATGGGACAGATTGCGTAATGTTAAGTGGTGAAACAGCTGCTGGAGCGTATCCAGAACTTGCTGTTAGAACAATGGCAAAAATATGTGTTGAAGCTGAAAGCACCCTTGATTATGGAGATGTCTTTAAAAGGATTATGGAACATTCCCCAGTTCCCATGAGCCCATTAGAAAGCCTAGCTTCTTCTGCTGTTAGAACTGCAAACTCAGCCAAGGCAGCTCTTATACTTGTCCTAACCAGAGGAGGAAGCACTGCAAAACTGGTGGCCAAGTATAGGCCAGGAACGCCCATCTTGTCTGTGGTTGTCCCCGAGATTAAGACAGATTCATTTGATTGGTCATGCAGCGATGAAGCTCCGGCAAGGCATAGCTTAATTTTTCGTGGGTTGATACCGGTTCTTAGCACAGCATCGGCAAGGGCGTCTCATGCAGAGACAACAGAAGAAGCCATAGAGTTCGCTATTCAGCATGCGAAGTCGAAGGGGCTCTGCAAAAATGGAGATTCTGTCGTGGCTCTTCATCGCGTTGGAACAGCATCTGTTATCAAGATATTGACTGTGAAGTAG

Protein sequence

MDGLLIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRSAMENTGILCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKKLAEDVKPGSIILCADVLGERKNVNLPGVIVDLPTLTDKDKEDILEWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSCSDEAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGDSVVALHRVGTASVIKILTVK
Homology
BLAST of Sgr001405 vs. NCBI nr
Match: XP_038901386.1 (pyruvate kinase, cytosolic isozyme [Benincasa hispida])

HSP 1 Score: 901.0 bits (2327), Expect = 4.5e-258
Identity = 466/500 (93.20%), Postives = 474/500 (94.80%), Query Frame = 0

Query: 1   MDGLLIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRS 60
           MDGL+IEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLR+
Sbjct: 1   MDGLVIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRA 60

Query: 61  AMENTGILCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKK 120
            MENTGILCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKK
Sbjct: 61  GMENTGILCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKK 120

Query: 121 LAEDVKPGSIILCAD-------------------------VLGERKNVNLPGVIVDLPTL 180
           LAEDVKPGS+ILCAD                         VLGERKNVNLPGVIVDLPTL
Sbjct: 121 LAEDVKPGSVILCADGTISFSVLSCDKKLGLVRCRCENSAVLGERKNVNLPGVIVDLPTL 180

Query: 181 TDKDKEDILEWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF 240
           TDKDKEDILEWG+PNKIDMIALSFVRKGSDLVEVRKLLGKHAK+ILLMSKVENQEGVANF
Sbjct: 181 TDKDKEDILEWGVPNKIDMIALSFVRKGSDLVEVRKLLGKHAKNILLMSKVENQEGVANF 240

Query: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300
           DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP
Sbjct: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300

Query: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME 360
           TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME
Sbjct: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME 360

Query: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420
           HSPVPMSPLESLASSAVRTANSAKA+LILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT
Sbjct: 361 HSPVPMSPLESLASSAVRTANSAKASLILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420

Query: 421 DSFDWSCSDEAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGD 476
           DSFDWSCSDEAPARHSLIFRGL+PVLSTASAR+SHAETTEEAIEFAIQHAKSKGLCKNGD
Sbjct: 421 DSFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKSKGLCKNGD 480

BLAST of Sgr001405 vs. NCBI nr
Match: XP_004149636.1 (pyruvate kinase, cytosolic isozyme [Cucumis sativus] >KGN54016.1 hypothetical protein Csa_021658 [Cucumis sativus])

HSP 1 Score: 899.0 bits (2322), Expect = 1.7e-257
Identity = 465/500 (93.00%), Postives = 473/500 (94.60%), Query Frame = 0

Query: 1   MDGLLIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRS 60
           MDGL+IEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLR+
Sbjct: 1   MDGLVIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRA 60

Query: 61  AMENTGILCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKK 120
            MENTGILCAVMLDTKGPEIRTGFLKD KPIQLKQGQEITISTDYSLKGDENMICMSYKK
Sbjct: 61  GMENTGILCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYSLKGDENMICMSYKK 120

Query: 121 LAEDVKPGSIILCAD-------------------------VLGERKNVNLPGVIVDLPTL 180
           LAEDVKPGS+ILC+D                         VLGERKNVNLPGVIVDLPTL
Sbjct: 121 LAEDVKPGSVILCSDGTISFSVLSCDKKLGLVQCRCENSAVLGERKNVNLPGVIVDLPTL 180

Query: 181 TDKDKEDILEWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF 240
           T+KDKEDILEWG+PNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF
Sbjct: 181 TEKDKEDILEWGVPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF 240

Query: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300
           DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP
Sbjct: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300

Query: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME 360
           TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME
Sbjct: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME 360

Query: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420
           HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT
Sbjct: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420

Query: 421 DSFDWSCSDEAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGD 476
           DSFDWSCSDEAPARHSLIFRGL+PVLSTASAR+SHAETTEEAIEFAIQHAKSKGLCKNGD
Sbjct: 421 DSFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKSKGLCKNGD 480

BLAST of Sgr001405 vs. NCBI nr
Match: XP_008449903.1 (PREDICTED: pyruvate kinase, cytosolic isozyme [Cucumis melo] >KAA0040121.1 pyruvate kinase, cytosolic isozyme [Cucumis melo var. makuwa] >TYK21743.1 pyruvate kinase, cytosolic isozyme [Cucumis melo var. makuwa])

HSP 1 Score: 896.3 bits (2315), Expect = 1.1e-256
Identity = 464/500 (92.80%), Postives = 472/500 (94.40%), Query Frame = 0

Query: 1   MDGLLIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRS 60
           MDGL+IEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLR+
Sbjct: 1   MDGLVIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRA 60

Query: 61  AMENTGILCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKK 120
            MENTGILCAVMLDTKGPEIRTGFLKD KPIQLKQGQEITISTDYSLKGDENMICMSYKK
Sbjct: 61  GMENTGILCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYSLKGDENMICMSYKK 120

Query: 121 LAEDVKPGSIILCAD-------------------------VLGERKNVNLPGVIVDLPTL 180
           LAEDVKPGS+ILC+D                         VLGERKNVNLPGVIVDLPTL
Sbjct: 121 LAEDVKPGSVILCSDGTISFSVLSCDKKLGLVRCRCENSAVLGERKNVNLPGVIVDLPTL 180

Query: 181 TDKDKEDILEWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF 240
           T+KDKEDILEWG+PNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF
Sbjct: 181 TEKDKEDILEWGVPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF 240

Query: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300
           DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP
Sbjct: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300

Query: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME 360
           TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTL YGDVFKRIME
Sbjct: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLHYGDVFKRIME 360

Query: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420
           HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT
Sbjct: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420

Query: 421 DSFDWSCSDEAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGD 476
           DSFDWSCSDEAPARHSLIFRGL+PVLSTASAR+SHAETTEEAIEFAIQHAKSKGLCKNGD
Sbjct: 421 DSFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKSKGLCKNGD 480

BLAST of Sgr001405 vs. NCBI nr
Match: XP_023512551.1 (pyruvate kinase, cytosolic isozyme-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 888.6 bits (2295), Expect = 2.3e-254
Identity = 459/500 (91.80%), Postives = 471/500 (94.20%), Query Frame = 0

Query: 1   MDGLLIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRS 60
           M+GL+IEKKPKTKIVCTLGPASRSVP+IEKLLKAGMNVARFNFSHGSHAYHQETLDNLR+
Sbjct: 1   MEGLVIEKKPKTKIVCTLGPASRSVPIIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRA 60

Query: 61  AMENTGILCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKK 120
           AME TGILCAVMLDTKGPEIRTGFLKD KPIQLKQ QEI ISTDYSLKGDENMICMSYKK
Sbjct: 61  AMEETGILCAVMLDTKGPEIRTGFLKDGKPIQLKQEQEIIISTDYSLKGDENMICMSYKK 120

Query: 121 LAEDVKPGSIILCAD-------------------------VLGERKNVNLPGVIVDLPTL 180
           LAEDVKPGS+ILC+D                         VLGERKNVNLPGVIVDLPTL
Sbjct: 121 LAEDVKPGSVILCSDGTISFKVLSCDKKLGLVRCRCENSAVLGERKNVNLPGVIVDLPTL 180

Query: 181 TDKDKEDILEWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF 240
           TDKDKEDILEWG+PNKIDMIALSFVRKGSDLV+VRKLLGKHAK+ILLMSKVENQEGVANF
Sbjct: 181 TDKDKEDILEWGVPNKIDMIALSFVRKGSDLVQVRKLLGKHAKTILLMSKVENQEGVANF 240

Query: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300
           DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP
Sbjct: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300

Query: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME 360
           TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME
Sbjct: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME 360

Query: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420
           HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT
Sbjct: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420

Query: 421 DSFDWSCSDEAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGD 476
           DSFDWSCSDEAPARHSLIFRGL+PVLSTASAR+SHAETTEEAIEFAIQHAKSKGLCKNGD
Sbjct: 421 DSFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKSKGLCKNGD 480

BLAST of Sgr001405 vs. NCBI nr
Match: XP_022944286.1 (pyruvate kinase, cytosolic isozyme-like [Cucurbita moschata] >KAG7010207.1 hypothetical protein SDJN02_26999 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 888.6 bits (2295), Expect = 2.3e-254
Identity = 459/500 (91.80%), Postives = 471/500 (94.20%), Query Frame = 0

Query: 1   MDGLLIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRS 60
           M+GL+IEKKPKTKIVCTLGPASRSVP+IEKLLKAGMNVARFNFSHGSHAYHQETLDNLR+
Sbjct: 1   MEGLVIEKKPKTKIVCTLGPASRSVPIIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRA 60

Query: 61  AMENTGILCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKK 120
           AME TGILCAVMLDTKGPEIRTGFLKD KPIQLKQ QEI ISTDYSLKGDENMICMSYKK
Sbjct: 61  AMEETGILCAVMLDTKGPEIRTGFLKDGKPIQLKQEQEIIISTDYSLKGDENMICMSYKK 120

Query: 121 LAEDVKPGSIILCAD-------------------------VLGERKNVNLPGVIVDLPTL 180
           LAEDVKPGS+ILC+D                         VLGERKNVNLPGVIVDLPTL
Sbjct: 121 LAEDVKPGSVILCSDGTISFTVLSCDKKLGLVRCRCENSAVLGERKNVNLPGVIVDLPTL 180

Query: 181 TDKDKEDILEWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF 240
           TDKDKEDILEWG+PNKIDMIALSFVRKGSDLV+VRKLLGKHAK+ILLMSKVENQEGVANF
Sbjct: 181 TDKDKEDILEWGVPNKIDMIALSFVRKGSDLVQVRKLLGKHAKTILLMSKVENQEGVANF 240

Query: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300
           DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP
Sbjct: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300

Query: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME 360
           TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME
Sbjct: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME 360

Query: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420
           HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT
Sbjct: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420

Query: 421 DSFDWSCSDEAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGD 476
           DSFDWSCSDEAPARHSLIFRGL+PVLSTASAR+SHAETTEEAIEFAIQHAKSKGLCKNGD
Sbjct: 421 DSFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKSKGLCKNGD 480

BLAST of Sgr001405 vs. ExPASy Swiss-Prot
Match: Q42954 (Pyruvate kinase, cytosolic isozyme OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 1.1e-240
Identity = 426/493 (86.41%), Postives = 453/493 (91.89%), Query Frame = 0

Query: 8   KKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRSAMENTGI 67
           K+PKTKIVCTLGPASRSVPMIEKLL+AGMNVARFNFSHGSH YHQET+DNLR AME+TGI
Sbjct: 16  KRPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGSHDYHQETIDNLRQAMESTGI 75

Query: 68  LCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKKLAEDVKP 127
           LCAVMLDTKGPEIRTGFLKD+KP+QLKQGQEITISTDYS+KGDE+MICMSYKKLAEDVKP
Sbjct: 76  LCAVMLDTKGPEIRTGFLKDAKPVQLKQGQEITISTDYSIKGDESMICMSYKKLAEDVKP 135

Query: 128 GSIILCAD-------------------------VLGERKNVNLPGVIVDLPTLTDKDKED 187
            S+ILCAD                         VLGERKNVNLPGVIVDLPTLTDKDK+D
Sbjct: 136 QSVILCADGQITFTVLSCDKENGLDRCRCENTAVLGERKNVNLPGVIVDLPTLTDKDKDD 195

Query: 188 ILEWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDILANS 247
           IL WG+PN IDMIALSFVRKGSDLVEVRKLLG+HAK+ILLMSKVENQEGVANFDDIL NS
Sbjct: 196 ILNWGVPNHIDMIALSFVRKGSDLVEVRKLLGEHAKNILLMSKVENQEGVANFDDILLNS 255

Query: 248 DAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATD 307
           DAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATD
Sbjct: 256 DAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATD 315

Query: 308 VANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSPVPMS 367
           VANAVLDGTDCVMLSGETAAGAYP+LAV TMAKIC+EAEST+DY DVFKRIM ++PVPMS
Sbjct: 316 VANAVLDGTDCVMLSGETAAGAYPDLAVGTMAKICIEAESTIDYPDVFKRIMSNAPVPMS 375

Query: 368 PLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSC 427
           PLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPG PILSVVVPEIKTDSFDW+C
Sbjct: 376 PLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGMPILSVVVPEIKTDSFDWTC 435

Query: 428 SDEAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGDSVVALHR 476
           SDE+PARHSLIFRGL+PVL   SARASH E+TEEA++FA+QHAK+KGLCK GDSVVALHR
Sbjct: 436 SDESPARHSLIFRGLVPVLHAGSARASHEESTEEALDFALQHAKTKGLCKQGDSVVALHR 495

BLAST of Sgr001405 vs. ExPASy Swiss-Prot
Match: O65595 (Probable pyruvate kinase, cytosolic isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g26390 PE=3 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 2.7e-226
Identity = 401/497 (80.68%), Postives = 437/497 (87.93%), Query Frame = 0

Query: 4   LLIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRSAME 63
           + +E++PKTKIVCTLGPASRSVPM+EKLL AGM+VARFNFSHGS+ YHQETLDNLR AM 
Sbjct: 1   MAMEQRPKTKIVCTLGPASRSVPMVEKLLMAGMSVARFNFSHGSYEYHQETLDNLRQAML 60

Query: 64  NTGILCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKKLAE 123
           NTG+LCAVMLDTKGPEIRTGFLKD KPIQLKQGQEITISTDY LKGDE  ICMSYKKLA+
Sbjct: 61  NTGMLCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDEKTICMSYKKLAQ 120

Query: 124 DVKPGSIILCAD-------------------------VLGERKNVNLPGVIVDLPTLTDK 183
           DV PG +ILCAD                         +LGERKNVNLPGV+VDLPTLT+K
Sbjct: 121 DVNPGMVILCADGTISLKVLSCDKEKGTVRCRCENTSMLGERKNVNLPGVVVDLPTLTEK 180

Query: 184 DKEDILEWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDI 243
           DK+DILEWG+PN+IDMIALSFVRKGSDLV+VRKLLGKHAK+ILLMSKVENQEGVANFDDI
Sbjct: 181 DKQDILEWGVPNQIDMIALSFVRKGSDLVQVRKLLGKHAKTILLMSKVENQEGVANFDDI 240

Query: 244 LANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRA 303
           L NSDAFM+ARGDLGMEIPIEKIFLAQKVMIYKCN  GKPVVTATQMLESMIKSPRPTRA
Sbjct: 241 LINSDAFMIARGDLGMEIPIEKIFLAQKVMIYKCNFMGKPVVTATQMLESMIKSPRPTRA 300

Query: 304 EATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSP 363
           EATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGD+FKRIM H+ 
Sbjct: 301 EATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDIFKRIMLHAA 360

Query: 364 VPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSF 423
           VPMSP+ESLASSAVRTA S++A L++VLTRGGSTA+LVAKYRPG PILSVVVPEI +DSF
Sbjct: 361 VPMSPMESLASSAVRTATSSRATLMMVLTRGGSTARLVAKYRPGIPILSVVVPEITSDSF 420

Query: 424 DWSCSDEAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGDSVV 476
           DW+CS+EAPARHSLI+RGL+PVL   SARAS  E+TEE +EFA ++ K K LCK GDSVV
Sbjct: 421 DWACSNEAPARHSLIYRGLVPVLYAGSARASIDESTEETLEFASEYGKKKQLCKTGDSVV 480

BLAST of Sgr001405 vs. ExPASy Swiss-Prot
Match: Q42806 (Pyruvate kinase, cytosolic isozyme OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 6.1e-210
Identity = 371/491 (75.56%), Postives = 417/491 (84.93%), Query Frame = 0

Query: 10  PKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRSAMENTGILC 69
           PKTKIVCTLGPASRSV M EKLL+AGMNVARFNFSHG+H YHQETL+NL++AM NTGILC
Sbjct: 21  PKTKIVCTLGPASRSVEMTEKLLRAGMNVARFNFSHGTHDYHQETLNNLKTAMHNTGILC 80

Query: 70  AVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKKLAEDVKPGS 129
           AVMLDTKGPEIRTGFLKD KPIQLK+GQE+TI+TDY +KGD  MI MSYKKL   +KPG+
Sbjct: 81  AVMLDTKGPEIRTGFLKDGKPIQLKEGQEVTITTDYDIKGDPEMISMSYKKLPVHLKPGN 140

Query: 130 IILCAD-------------------------VLGERKNVNLPGVIVDLPTLTDKDKEDIL 189
            ILC+D                          LGERKNVNLPGV+VDLPTLT+KDKEDIL
Sbjct: 141 TILCSDGTITLTVLSCDPDAGTVRCRCENTATLGERKNVNLPGVVVDLPTLTEKDKEDIL 200

Query: 190 EWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDILANSDA 249
            WG+PNKIDMIALSFVRKGSDLV VRK+LG HAK+I LMSKVENQEGV NFD+IL  +DA
Sbjct: 201 GWGVPNKIDMIALSFVRKGSDLVNVRKVLGPHAKNIQLMSKVENQEGVLNFDEILRETDA 260

Query: 250 FMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVA 309
           FMVARGDLGMEIP+EKIFLAQK+MIYKCN+ GKPVVTATQMLESMIKSPRPTRAEATDVA
Sbjct: 261 FMVARGDLGMEIPVEKIFLAQKMMIYKCNLVGKPVVTATQMLESMIKSPRPTRAEATDVA 320

Query: 310 NAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSPVPMSPL 369
           NAVLDGTDCVMLSGE+AAGAYPELAV+ MA+IC+EAES+LDYG +FK ++  +P+PMSPL
Sbjct: 321 NAVLDGTDCVMLSGESAAGAYPELAVKIMARICIEAESSLDYGAIFKEMIRSTPLPMSPL 380

Query: 370 ESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSCSD 429
           ESLASSAVRTAN AKA LI+VLTRGGSTAKLVAKYRP  PILSVVVP + TDSFDW+CSD
Sbjct: 381 ESLASSAVRTANKAKAKLIVVLTRGGSTAKLVAKYRPAVPILSVVVPVLSTDSFDWTCSD 440

Query: 430 EAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGDSVVALHRVG 476
           E PARHSLI+RGLIP+L   SA+A+ AE+TE  +E A++ A  + LCK GD+VVALHR+G
Sbjct: 441 ETPARHSLIYRGLIPILGEGSAKATDAESTEVILEAALKSATERALCKPGDAVVALHRIG 500

BLAST of Sgr001405 vs. ExPASy Swiss-Prot
Match: P22200 (Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 7.0e-206
Identity = 364/491 (74.13%), Postives = 417/491 (84.93%), Query Frame = 0

Query: 10  PKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRSAMENTGILC 69
           PKTKIVCTLGP+SR+VPM+EKLL+AGMNVARFNFSHG+H YHQETLDNL+ AM+NT ILC
Sbjct: 20  PKTKIVCTLGPSSRTVPMLEKLLRAGMNVARFNFSHGTHEYHQETLDNLKIAMQNTQILC 79

Query: 70  AVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKKLAEDVKPGS 129
           AVMLDTKGPEIRTGFL D KPIQLK+GQEIT+STDY++KG+E MI MSYKKL  D+KPG+
Sbjct: 80  AVMLDTKGPEIRTGFLTDGKPIQLKEGQEITVSTDYTIKGNEEMISMSYKKLVMDLKPGN 139

Query: 130 IILCAD-------------------------VLGERKNVNLPGVIVDLPTLTDKDKEDIL 189
            ILCAD                          LGERKNVNLPGV+VDLPTLT+KDKEDIL
Sbjct: 140 TILCADGTITLTVLSCDPPSGTVRCRCENTATLGERKNVNLPGVVVDLPTLTEKDKEDIL 199

Query: 190 EWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDILANSDA 249
           EWG+PN IDMIALSFVRKGSDLV VRK+LG HAK I LMSKVENQEGV NFD+IL  +D+
Sbjct: 200 EWGVPNNIDMIALSFVRKGSDLVNVRKVLGPHAKRIQLMSKVENQEGVINFDEILRETDS 259

Query: 250 FMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVA 309
           FMVARGDLGMEIP+EKIFLAQK+MIYKCN+ GK VVTATQMLESMIKSP PTRAEATDVA
Sbjct: 260 FMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKAVVTATQMLESMIKSPAPTRAEATDVA 319

Query: 310 NAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSPVPMSPL 369
           NAVLDGTDCVMLSGE+AAGAYPELAV+ M++IC+EAES+LD   +FK ++  +P+PMSPL
Sbjct: 320 NAVLDGTDCVMLSGESAAGAYPELAVKIMSRICIEAESSLDNEAIFKEMIRCTPLPMSPL 379

Query: 370 ESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSCSD 429
           ESLASSAVRTAN A+A LI+VLTRGGSTAKLVAKYRP  PILSVVVP + TDSFDWS SD
Sbjct: 380 ESLASSAVRTANKARAKLIVVLTRGGSTAKLVAKYRPAVPILSVVVPVLTTDSFDWSISD 439

Query: 430 EAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGDSVVALHRVG 476
           E PARHSL++RGLIP+L   SA+A+ +E+TE  +E A++ A ++GLCK GD+VVALHR+G
Sbjct: 440 ETPARHSLVYRGLIPLLGEGSAKATDSESTEVILEAALKSAVTRGLCKPGDAVVALHRIG 499

BLAST of Sgr001405 vs. ExPASy Swiss-Prot
Match: O44006 (Pyruvate kinase OS=Eimeria tenella OX=5802 GN=PYK PE=2 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 8.8e-116
Identity = 232/494 (46.96%), Postives = 313/494 (63.36%), Query Frame = 0

Query: 11  KTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRSAM-ENTGILC 70
           +TKIVCT+GP+   V  + +L+ AGMNV R NFSHG H  H   + NL+ A+ +  G   
Sbjct: 57  RTKIVCTMGPSCWDVDKMVQLIDAGMNVCRLNFSHGDHEAHGRVVKNLQEALKQRPGKRV 116

Query: 71  AVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKKLAEDVKPGS 130
           A++LDTKGPEIRTG L+  KPI+L  G  + I TDYS  G+++ I  SY+KL   VKPG+
Sbjct: 117 ALLLDTKGPEIRTGMLEGDKPIELHAGDMLKIVTDYSFVGNKSCIACSYEKLPSSVKPGN 176

Query: 131 IILCAD-----------------------VLGERKNVNLPGVIVDLPTLTDKDKEDILEW 190
            IL AD                       ++G +KN+NLPGV VDLP + +KDK DIL +
Sbjct: 177 TILIADGSLSVEVVECGKDYVMTRVMNPAIIGNKKNMNLPGVKVDLPVIGEKDKNDILNF 236

Query: 191 GIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDILANSDAFM 250
           GIP   + IA SFV+   D+  +R +LG   ++I ++ K+EN EG+ NFD+IL  +D  M
Sbjct: 237 GIPMGCNFIAASFVQSADDVRYIRSILGTKGRNIKIIPKIENVEGLLNFDEILQEADGIM 296

Query: 251 VARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANA 310
           +ARGDLGMEIP EK+FLAQK+MI KCN+ GKPV+TATQMLESM K+PRPTRAEA DVANA
Sbjct: 297 IARGDLGMEIPPEKVFLAQKMMISKCNVAGKPVITATQMLESMTKNPRPTRAEAADVANA 356

Query: 311 VLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSPVPMSPLES 370
           VLDGTDCVMLSGETA G++P  AV  M+++C EAE  +DY  VF+   + +  P+   E+
Sbjct: 357 VLDGTDCVMLSGETANGSFPVQAVTVMSRVCFEAEGCIDYQQVFRATCQATMTPIDTQEA 416

Query: 371 LASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSCSDEA 430
           +A +AV TA S  A+LIL LT  G TA+L+AKYRP  PIL++            S S+E 
Sbjct: 417 VARAAVETAQSINASLILALTETGRTARLIAKYRPMQPILAL------------SASEET 476

Query: 431 PARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGDSVVALHRV--- 476
             +   + RG+   L          + T++ I  A+  AK   L   GDS+VA+H +   
Sbjct: 477 -IKQLQVIRGVTTFL------VPTFQGTDQLIRNALSAAKELQLVSEGDSIVAVHGIKEE 531

BLAST of Sgr001405 vs. ExPASy TrEMBL
Match: A0A0A0L1N0 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G268000 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 8.2e-258
Identity = 465/500 (93.00%), Postives = 473/500 (94.60%), Query Frame = 0

Query: 1   MDGLLIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRS 60
           MDGL+IEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLR+
Sbjct: 1   MDGLVIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRA 60

Query: 61  AMENTGILCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKK 120
            MENTGILCAVMLDTKGPEIRTGFLKD KPIQLKQGQEITISTDYSLKGDENMICMSYKK
Sbjct: 61  GMENTGILCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYSLKGDENMICMSYKK 120

Query: 121 LAEDVKPGSIILCAD-------------------------VLGERKNVNLPGVIVDLPTL 180
           LAEDVKPGS+ILC+D                         VLGERKNVNLPGVIVDLPTL
Sbjct: 121 LAEDVKPGSVILCSDGTISFSVLSCDKKLGLVQCRCENSAVLGERKNVNLPGVIVDLPTL 180

Query: 181 TDKDKEDILEWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF 240
           T+KDKEDILEWG+PNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF
Sbjct: 181 TEKDKEDILEWGVPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF 240

Query: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300
           DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP
Sbjct: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300

Query: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME 360
           TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME
Sbjct: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME 360

Query: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420
           HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT
Sbjct: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420

Query: 421 DSFDWSCSDEAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGD 476
           DSFDWSCSDEAPARHSLIFRGL+PVLSTASAR+SHAETTEEAIEFAIQHAKSKGLCKNGD
Sbjct: 421 DSFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKSKGLCKNGD 480

BLAST of Sgr001405 vs. ExPASy TrEMBL
Match: A0A5D3DE01 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001150 PE=3 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 5.3e-257
Identity = 464/500 (92.80%), Postives = 472/500 (94.40%), Query Frame = 0

Query: 1   MDGLLIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRS 60
           MDGL+IEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLR+
Sbjct: 1   MDGLVIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRA 60

Query: 61  AMENTGILCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKK 120
            MENTGILCAVMLDTKGPEIRTGFLKD KPIQLKQGQEITISTDYSLKGDENMICMSYKK
Sbjct: 61  GMENTGILCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYSLKGDENMICMSYKK 120

Query: 121 LAEDVKPGSIILCAD-------------------------VLGERKNVNLPGVIVDLPTL 180
           LAEDVKPGS+ILC+D                         VLGERKNVNLPGVIVDLPTL
Sbjct: 121 LAEDVKPGSVILCSDGTISFSVLSCDKKLGLVRCRCENSAVLGERKNVNLPGVIVDLPTL 180

Query: 181 TDKDKEDILEWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF 240
           T+KDKEDILEWG+PNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF
Sbjct: 181 TEKDKEDILEWGVPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF 240

Query: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300
           DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP
Sbjct: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300

Query: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME 360
           TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTL YGDVFKRIME
Sbjct: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLHYGDVFKRIME 360

Query: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420
           HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT
Sbjct: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420

Query: 421 DSFDWSCSDEAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGD 476
           DSFDWSCSDEAPARHSLIFRGL+PVLSTASAR+SHAETTEEAIEFAIQHAKSKGLCKNGD
Sbjct: 421 DSFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKSKGLCKNGD 480

BLAST of Sgr001405 vs. ExPASy TrEMBL
Match: A0A1S3BN43 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103491640 PE=3 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 5.3e-257
Identity = 464/500 (92.80%), Postives = 472/500 (94.40%), Query Frame = 0

Query: 1   MDGLLIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRS 60
           MDGL+IEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLR+
Sbjct: 1   MDGLVIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRA 60

Query: 61  AMENTGILCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKK 120
            MENTGILCAVMLDTKGPEIRTGFLKD KPIQLKQGQEITISTDYSLKGDENMICMSYKK
Sbjct: 61  GMENTGILCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYSLKGDENMICMSYKK 120

Query: 121 LAEDVKPGSIILCAD-------------------------VLGERKNVNLPGVIVDLPTL 180
           LAEDVKPGS+ILC+D                         VLGERKNVNLPGVIVDLPTL
Sbjct: 121 LAEDVKPGSVILCSDGTISFSVLSCDKKLGLVRCRCENSAVLGERKNVNLPGVIVDLPTL 180

Query: 181 TDKDKEDILEWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF 240
           T+KDKEDILEWG+PNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF
Sbjct: 181 TEKDKEDILEWGVPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF 240

Query: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300
           DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP
Sbjct: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300

Query: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME 360
           TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTL YGDVFKRIME
Sbjct: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLHYGDVFKRIME 360

Query: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420
           HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT
Sbjct: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420

Query: 421 DSFDWSCSDEAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGD 476
           DSFDWSCSDEAPARHSLIFRGL+PVLSTASAR+SHAETTEEAIEFAIQHAKSKGLCKNGD
Sbjct: 421 DSFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKSKGLCKNGD 480

BLAST of Sgr001405 vs. ExPASy TrEMBL
Match: A0A6J1K6R4 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111491658 PE=3 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.1e-254
Identity = 459/500 (91.80%), Postives = 470/500 (94.00%), Query Frame = 0

Query: 1   MDGLLIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRS 60
           MDGL+IEKKPKTKIVCTLGPASRSVPM+EKLLKAGMNVARFNFSHGSH YHQETLDNLR+
Sbjct: 1   MDGLVIEKKPKTKIVCTLGPASRSVPMVEKLLKAGMNVARFNFSHGSHEYHQETLDNLRA 60

Query: 61  AMENTGILCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKK 120
           AMENTGILCAVMLDTKGPEIRTGFLKD KPIQL QGQEITISTDYSLKGDENMICMSYKK
Sbjct: 61  AMENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKK 120

Query: 121 LAEDVKPGSIILCAD-------------------------VLGERKNVNLPGVIVDLPTL 180
           LAEDVKPGS+ILC+D                         VLGERKNVNLPGVIVDLPTL
Sbjct: 121 LAEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTL 180

Query: 181 TDKDKEDILEWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF 240
           T+KDKEDILEWG+PN IDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF
Sbjct: 181 TEKDKEDILEWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF 240

Query: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300
           DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP
Sbjct: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300

Query: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME 360
           TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTL+YGDVFKRIME
Sbjct: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIME 360

Query: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420
           HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT
Sbjct: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420

Query: 421 DSFDWSCSDEAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGD 476
           DSFDWSCSDEAPARHSLIFRGL+PVLSTASA++SHAETTEEAIEFAIQHAK KGLCKNGD
Sbjct: 421 DSFDWSCSDEAPARHSLIFRGLVPVLSTASAKSSHAETTEEAIEFAIQHAKLKGLCKNGD 480

BLAST of Sgr001405 vs. ExPASy TrEMBL
Match: A0A6J1H3K9 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111459794 PE=3 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.1e-254
Identity = 459/500 (91.80%), Postives = 470/500 (94.00%), Query Frame = 0

Query: 1   MDGLLIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRS 60
           MDGL+IEKKPKTKIVCTLGPASRSVPM+EKLLKAGMNVARFNFSHGSH YHQETLDNLR+
Sbjct: 1   MDGLVIEKKPKTKIVCTLGPASRSVPMVEKLLKAGMNVARFNFSHGSHEYHQETLDNLRA 60

Query: 61  AMENTGILCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKK 120
           AMENTGILCAVMLDTKGPEIRTGFLKD KPIQL QGQEITISTDYSLKGDENMICMSYKK
Sbjct: 61  AMENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKK 120

Query: 121 LAEDVKPGSIILCAD-------------------------VLGERKNVNLPGVIVDLPTL 180
           LAEDVKPGS+ILC+D                         VLGERKNVNLPGVIVDLPTL
Sbjct: 121 LAEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTL 180

Query: 181 TDKDKEDILEWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF 240
           T+KDKEDIL+WG+PN IDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF
Sbjct: 181 TEKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANF 240

Query: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300
           DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP
Sbjct: 241 DDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRP 300

Query: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME 360
           TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTL+YGDVFKRIME
Sbjct: 301 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIME 360

Query: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420
           HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT
Sbjct: 361 HSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKT 420

Query: 421 DSFDWSCSDEAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGD 476
           DSFDWSCSDEAPARHSLIFRGL+PVLSTASAR+SHAETTEEAIEFAIQHAK KGLCKNGD
Sbjct: 421 DSFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGD 480

BLAST of Sgr001405 vs. TAIR 10
Match: AT5G56350.1 (Pyruvate kinase family protein )

HSP 1 Score: 819.7 bits (2116), Expect = 1.2e-237
Identity = 422/496 (85.08%), Postives = 445/496 (89.72%), Query Frame = 0

Query: 5   LIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRSAMEN 64
           +IE++PKTKIVCTLGPASRSVPM+EKLL+AGMNVARFNFSHGSH YHQETLDNL  AM N
Sbjct: 3   MIEQRPKTKIVCTLGPASRSVPMVEKLLRAGMNVARFNFSHGSHEYHQETLDNLHQAMLN 62

Query: 65  TGILCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKKLAED 124
           TGILCAVMLDTKGPEIRTGFLKD KPIQLKQGQEITISTDY LKGDEN ICMSYKKLA D
Sbjct: 63  TGILCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDENTICMSYKKLAVD 122

Query: 125 VKPGSIILCAD-------------------------VLGERKNVNLPGVIVDLPTLTDKD 184
           V PG +ILCAD                         +LGERKNVNLPGV+VDLPTLT+KD
Sbjct: 123 VNPGMVILCADGTISLLVLSCDKENGTVRCRCENSAMLGERKNVNLPGVVVDLPTLTEKD 182

Query: 185 KEDILEWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDIL 244
           KEDI++WG+PN+IDMIALSFVRKGSDLV+VRKLLGKHAK+ILLMSKVENQEGVANFDDIL
Sbjct: 183 KEDIMQWGVPNQIDMIALSFVRKGSDLVQVRKLLGKHAKNILLMSKVENQEGVANFDDIL 242

Query: 245 ANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAE 304
            NSDAFM+ARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAE
Sbjct: 243 VNSDAFMIARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAE 302

Query: 305 ATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSPV 364
           ATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIM +SPV
Sbjct: 303 ATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMLYSPV 362

Query: 365 PMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFD 424
           PMSPLESLASSAVRTANSA+A LI+VLTRGGSTA+LVAKYRPG PILSVVVPEIKTD FD
Sbjct: 363 PMSPLESLASSAVRTANSARATLIMVLTRGGSTARLVAKYRPGMPILSVVVPEIKTDFFD 422

Query: 425 WSCSDEAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGDSVVA 476
           WSCSDE+PARHSLIFRGLIPVL   SARASH E+TEEAIEFA Q+ K K LCK GDSVVA
Sbjct: 423 WSCSDESPARHSLIFRGLIPVLYAGSARASHDESTEEAIEFATQYGKEKELCKTGDSVVA 482

BLAST of Sgr001405 vs. TAIR 10
Match: AT4G26390.1 (Pyruvate kinase family protein )

HSP 1 Score: 785.8 bits (2028), Expect = 2.0e-227
Identity = 401/497 (80.68%), Postives = 437/497 (87.93%), Query Frame = 0

Query: 4   LLIEKKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRSAME 63
           + +E++PKTKIVCTLGPASRSVPM+EKLL AGM+VARFNFSHGS+ YHQETLDNLR AM 
Sbjct: 1   MAMEQRPKTKIVCTLGPASRSVPMVEKLLMAGMSVARFNFSHGSYEYHQETLDNLRQAML 60

Query: 64  NTGILCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKKLAE 123
           NTG+LCAVMLDTKGPEIRTGFLKD KPIQLKQGQEITISTDY LKGDE  ICMSYKKLA+
Sbjct: 61  NTGMLCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDEKTICMSYKKLAQ 120

Query: 124 DVKPGSIILCAD-------------------------VLGERKNVNLPGVIVDLPTLTDK 183
           DV PG +ILCAD                         +LGERKNVNLPGV+VDLPTLT+K
Sbjct: 121 DVNPGMVILCADGTISLKVLSCDKEKGTVRCRCENTSMLGERKNVNLPGVVVDLPTLTEK 180

Query: 184 DKEDILEWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDI 243
           DK+DILEWG+PN+IDMIALSFVRKGSDLV+VRKLLGKHAK+ILLMSKVENQEGVANFDDI
Sbjct: 181 DKQDILEWGVPNQIDMIALSFVRKGSDLVQVRKLLGKHAKTILLMSKVENQEGVANFDDI 240

Query: 244 LANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRA 303
           L NSDAFM+ARGDLGMEIPIEKIFLAQKVMIYKCN  GKPVVTATQMLESMIKSPRPTRA
Sbjct: 241 LINSDAFMIARGDLGMEIPIEKIFLAQKVMIYKCNFMGKPVVTATQMLESMIKSPRPTRA 300

Query: 304 EATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSP 363
           EATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGD+FKRIM H+ 
Sbjct: 301 EATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDIFKRIMLHAA 360

Query: 364 VPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSF 423
           VPMSP+ESLASSAVRTA S++A L++VLTRGGSTA+LVAKYRPG PILSVVVPEI +DSF
Sbjct: 361 VPMSPMESLASSAVRTATSSRATLMMVLTRGGSTARLVAKYRPGIPILSVVVPEITSDSF 420

Query: 424 DWSCSDEAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGDSVV 476
           DW+CS+EAPARHSLI+RGL+PVL   SARAS  E+TEE +EFA ++ K K LCK GDSVV
Sbjct: 421 DWACSNEAPARHSLIYRGLVPVLYAGSARASIDESTEETLEFASEYGKKKQLCKTGDSVV 480

BLAST of Sgr001405 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 740.7 bits (1911), Expect = 7.2e-214
Identity = 380/505 (75.25%), Postives = 427/505 (84.55%), Query Frame = 0

Query: 1   MDGLLIE-----KKPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETL 60
           ++G+L E     + PKTKIVCTLGPASRSV MIEKLLKAGMNVARFNFSHGSH YHQETL
Sbjct: 6   IEGILKELPNDGRTPKTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSHEYHQETL 65

Query: 61  DNLRSAMENTGILCAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMIC 120
           DNLR+AM+NTGIL AVMLDTKGPEIRTGFLKD  PIQLK+GQEITI+TDY +KGDE  I 
Sbjct: 66  DNLRTAMQNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDIKGDEKTIS 125

Query: 121 MSYKKLAEDVKPGSIILCAD-------------------------VLGERKNVNLPGVIV 180
           MSYKKL  DVKPG+ ILCAD                         +LGERKNVNLPGV+V
Sbjct: 126 MSYKKLPVDVKPGNTILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKNVNLPGVVV 185

Query: 181 DLPTLTDKDKEDILEWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQE 240
           DLPTLTDKD EDIL+WG+PN IDMIALSFVRKGSDLV VRK+LG H+KSI+LMSKVENQE
Sbjct: 186 DLPTLTDKDVEDILKWGVPNNIDMIALSFVRKGSDLVNVRKVLGSHSKSIMLMSKVENQE 245

Query: 241 GVANFDDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMI 300
           GV NFD+IL  +DAFMVARGDLGMEIPIEKIFLAQK+MIYKCN+ GKPVVTATQMLESMI
Sbjct: 246 GVLNFDEILRETDAFMVARGDLGMEIPIEKIFLAQKMMIYKCNLAGKPVVTATQMLESMI 305

Query: 301 KSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVF 360
           KSPRPTRAEATDVANAVLDGTDCVMLSGE+AAGAYPE+AV+TMAKIC+EAES+LDY  +F
Sbjct: 306 KSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVKTMAKICIEAESSLDYNTIF 365

Query: 361 KRIMEHSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVV 420
           K ++  +P+PMS LESLASSAVRTAN AKA LI+VLTRGG+TAKLVAKYRP  PILSVVV
Sbjct: 366 KEMIRATPLPMSTLESLASSAVRTANKAKAKLIIVLTRGGTTAKLVAKYRPAVPILSVVV 425

Query: 421 PEIKTDSFDWSCSDEAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGL 476
           P   +D+F+WSCSDE+PARHSLI+RGLIPVL   SA+A+ +E+TEE IE A++ A  KGL
Sbjct: 426 PVFTSDTFNWSCSDESPARHSLIYRGLIPVLGEGSAKATDSESTEEIIESALKSATEKGL 485

BLAST of Sgr001405 vs. TAIR 10
Match: AT5G08570.1 (Pyruvate kinase family protein )

HSP 1 Score: 734.9 bits (1896), Expect = 3.9e-212
Identity = 375/491 (76.37%), Postives = 417/491 (84.93%), Query Frame = 0

Query: 10  PKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRSAMENTGILC 69
           PKTKIVCTLGPASR+V MIEKLLKAGMNVARFNFSHGSH YHQETLDNLRSAM NTGIL 
Sbjct: 20  PKTKIVCTLGPASRTVSMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLRSAMHNTGILA 79

Query: 70  AVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKKLAEDVKPGS 129
           AVMLDTKGPEIRTGFLKD  PIQLK+GQEITI+TDY ++GDE+ I MSYKKL  DVKPG+
Sbjct: 80  AVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDIQGDESTISMSYKKLPLDVKPGN 139

Query: 130 IILCAD-------------------------VLGERKNVNLPGVIVDLPTLTDKDKEDIL 189
            ILCAD                         +LGERKNVNLPGV+VDLPTLTDKD EDIL
Sbjct: 140 TILCADGSISLAVLSCDPESGTVRCRCENSAMLGERKNVNLPGVVVDLPTLTDKDIEDIL 199

Query: 190 EWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDILANSDA 249
            WG+PN IDMIALSFVRKGSDLV VRK+LG HAKSI+LMSKVENQEGV NFD+IL  +DA
Sbjct: 200 GWGVPNSIDMIALSFVRKGSDLVNVRKVLGSHAKSIMLMSKVENQEGVINFDEILRETDA 259

Query: 250 FMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVA 309
           FMVARGDLGMEIPIEKIFLAQK+MIYKCN+ GKPVVTATQMLESMIKSPRPTRAEATDVA
Sbjct: 260 FMVARGDLGMEIPIEKIFLAQKLMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVA 319

Query: 310 NAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSPVPMSPL 369
           NAVLDGTDCVMLSGE+AAGAYPE+AV+ MAKIC+EAES+LDY  +FK ++  +P+PMSPL
Sbjct: 320 NAVLDGTDCVMLSGESAAGAYPEIAVKVMAKICIEAESSLDYNTIFKEMIRATPLPMSPL 379

Query: 370 ESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSCSD 429
           ESLASSAVRTAN A+A LI+VLTRGGSTA LVAKYRP  PILSVVVP + TDSFDWSCSD
Sbjct: 380 ESLASSAVRTANKARAKLIIVLTRGGSTANLVAKYRPAVPILSVVVPVMTTDSFDWSCSD 439

Query: 430 EAPARHSLIFRGLIPVLSTASARASHAETTEEAIEFAIQHAKSKGLCKNGDSVVALHRVG 476
           E+PARHSLI+RGLIP+L+  SA+A+ +E TE  IE A++ A  +GLC  GD++VALHR+G
Sbjct: 440 ESPARHSLIYRGLIPMLAEGSAKATDSEATEVIIEAALKSATQRGLCNRGDAIVALHRIG 499

BLAST of Sgr001405 vs. TAIR 10
Match: AT3G04050.1 (Pyruvate kinase family protein )

HSP 1 Score: 680.6 bits (1755), Expect = 8.8e-196
Identity = 352/496 (70.97%), Postives = 413/496 (83.27%), Query Frame = 0

Query: 9   KPKTKIVCTLGPASRSVPMIEKLLKAGMNVARFNFSHGSHAYHQETLDNLRSAMENTGIL 68
           K KTKIVCTLGPASRSV M+EKLLKAGMNVARFNFSHGSH+YHQETLDNLR+AMENT I 
Sbjct: 15  KSKTKIVCTLGPASRSVEMVEKLLKAGMNVARFNFSHGSHSYHQETLDNLRTAMENTCIP 74

Query: 69  CAVMLDTKGPEIRTGFLKDSKPIQLKQGQEITISTDYSLKGDENMICMSYKKLAEDVKPG 128
           CAVMLDTKGPEIRTGFLK+ KP++L QGQEITISTDY+++GD N I MSYKKLAED+K G
Sbjct: 75  CAVMLDTKGPEIRTGFLKEGKPVELIQGQEITISTDYTMEGDSNTISMSYKKLAEDLKSG 134

Query: 129 SIILCAD-------------------------VLGERKNVNLPGVIVDLPTLTDKDKEDI 188
            +ILC+D                         VLGERKNVNLPG++VDLPTLT+KD+EDI
Sbjct: 135 DVILCSDGTISLTVLSCDKNLGLVRARCENSAVLGERKNVNLPGIVVDLPTLTEKDQEDI 194

Query: 189 LEWGIPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDILANSD 248
           L+WG+PNKID+IALSFVRKGSDLVEVRKLLG++AKSI+LMSKVENQEGV NFD IL  SD
Sbjct: 195 LQWGVPNKIDIIALSFVRKGSDLVEVRKLLGENAKSIMLMSKVENQEGVMNFDKILEYSD 254

Query: 249 AFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDV 308
           AFMVARGDLGMEIPIEK+FLAQK+MI K N  GKP+VTATQMLESM KSPRPTRAEATDV
Sbjct: 255 AFMVARGDLGMEIPIEKMFLAQKMMIQKANALGKPIVTATQMLESMTKSPRPTRAEATDV 314

Query: 309 ANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSPVPMSP 368
           ANAVLDGTDCVMLSGETAAGA+PE AV TM++IC EAE  +DY  + K+I +   +P+SP
Sbjct: 315 ANAVLDGTDCVMLSGETAAGAHPETAVLTMSRICKEAEDFIDYDTMHKKIQDIVSLPLSP 374

Query: 369 LESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEI-KTDSFDWSC 428
           +ESLA+SAV TA S  AA I+VLT+GG T +LVAKYRP  PILSV+VPEI +TD F+WSC
Sbjct: 375 IESLAASAVSTARSLCAAAIVVLTKGGYTVELVAKYRPSVPILSVIVPEITRTDDFEWSC 434

Query: 429 SDEAP--ARHSLIFRGLIPVLST-ASARASHAETTEEAIEFAIQHAKSKGLCKNGDSVVA 476
           S+ A   AR  LI+RG++PV++T ASAR+S+ ++TEE I+FAI+ AK KG+CK GDS+VA
Sbjct: 435 SESAAHVARRGLIYRGIVPVMATGASARSSNKDSTEETIQFAIEFAKKKGICKTGDSIVA 494

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038901386.14.5e-25893.20pyruvate kinase, cytosolic isozyme [Benincasa hispida][more]
XP_004149636.11.7e-25793.00pyruvate kinase, cytosolic isozyme [Cucumis sativus] >KGN54016.1 hypothetical pr... [more]
XP_008449903.11.1e-25692.80PREDICTED: pyruvate kinase, cytosolic isozyme [Cucumis melo] >KAA0040121.1 pyruv... [more]
XP_023512551.12.3e-25491.80pyruvate kinase, cytosolic isozyme-like [Cucurbita pepo subsp. pepo][more]
XP_022944286.12.3e-25491.80pyruvate kinase, cytosolic isozyme-like [Cucurbita moschata] >KAG7010207.1 hypot... [more]
Match NameE-valueIdentityDescription
Q429541.1e-24086.41Pyruvate kinase, cytosolic isozyme OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
O655952.7e-22680.68Probable pyruvate kinase, cytosolic isozyme OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q428066.1e-21075.56Pyruvate kinase, cytosolic isozyme OS=Glycine max OX=3847 PE=2 SV=1[more]
P222007.0e-20674.13Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
O440068.8e-11646.96Pyruvate kinase OS=Eimeria tenella OX=5802 GN=PYK PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L1N08.2e-25893.00Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G268000 PE=3 SV=1[more]
A0A5D3DE015.3e-25792.80Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G0011... [more]
A0A1S3BN435.3e-25792.80Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103491640 PE=3 SV=1[more]
A0A6J1K6R41.1e-25491.80Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111491658 PE=3 SV=1[more]
A0A6J1H3K91.1e-25491.80Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111459794 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G56350.11.2e-23785.08Pyruvate kinase family protein [more]
AT4G26390.12.0e-22780.68Pyruvate kinase family protein [more]
AT5G63680.17.2e-21475.25Pyruvate kinase family protein [more]
AT5G08570.13.9e-21276.37Pyruvate kinase family protein [more]
AT3G04050.18.8e-19670.97Pyruvate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 172..186
score: 51.79
coord: 254..278
score: 72.25
coord: 202..228
score: 51.68
coord: 279..297
score: 77.25
coord: 229..253
score: 63.51
coord: 298..314
score: 58.37
coord: 67..83
score: 62.9
IPR001697Pyruvate kinaseTIGRFAMTIGR01064TIGR01064coord: 136..464
e-value: 1.6E-102
score: 341.4
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 5..135
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 136..475
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 136..328
e-value: 1.4E-94
score: 316.9
coord: 11..135
e-value: 3.9E-46
score: 157.5
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 10..132
e-value: 4.3E-33
score: 116.7
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 133..320
e-value: 3.6E-85
score: 287.1
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 345..471
e-value: 1.8E-22
score: 79.7
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3D3.40.1380.20coord: 329..475
e-value: 1.6E-31
score: 110.5
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILY52935PK C-terminal domain-likecoord: 309..474
NoneNo IPR availablePANTHERPTHR11817:SF90PYRUVATE KINASEcoord: 5..135
NoneNo IPR availablePANTHERPTHR11817:SF90PYRUVATE KINASEcoord: 136..475
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 200..212
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 10..326
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILY50800PK beta-barrel domain-likecoord: 78..152

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr001405.1Sgr001405.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0003824 catalytic activity